FG535330

EST Overview
NameFG535330
Unique NameFG535330
TypeEST
OrganismPisum sativum (pea)
Sequence length450
Libraries
Library NameType
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Pisum sativum unigene v22011-03-01
Homology Analysis: Pisum sativum unigene v2 vs Trembl2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins2011-04-21
Homology
[Close]
BLAST of FG535330 vs. TrEMBL
Match: D7SVC2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032922001 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.090e-15
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 2 
Query:   11 KFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPE 181
            KF  L+LPK DE+WSIA EKPKEGD LIEHV E++ IE+KRKALE AL +K+   ++ PE
Sbjct:  509 KFISLDLPKPDEVWSIATEKPKEGDTLIEHVIEKEIIEKKRKALERALHRKTIQWQQTPE 568

HSP 2 Score: 28.8758 bits (63), Expect = 4.090e-15
Identity = 12/15 (80.00%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  285 DANAEPGTGTGREIV 329
            D   EPGTGTGREIV
Sbjct:  569 DTQLEPGTGTGREIV 583
[Close]
BLAST of FG535330 vs. TrEMBL
Match: B9SJF1_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_0526780 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.908e-15
Identity = 37/61 (60.66%), Postives = 49/61 (80.33%), Query Frame = 2 
Query:    8 RKFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPE 181
            + F+ L+LPK DE+WSIA+EKPK GD LIEHV E++TIE+KRKALE  L++K+   +R PE
Sbjct:  505 KNFRHLDLPKPDEVWSIAQEKPKVGDTLIEHVMEKETIEKKRKALERVLQRKTIQWQRTPE 565

HSP 2 Score: 26.9498 bits (58), Expect = 6.908e-15
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  297 EPGTGTGREIV 329
            EPGTGTGREIV
Sbjct:  570 EPGTGTGREIV 580
[Close]
BLAST of FG535330 vs. TrEMBL
Match: E5GCI0_CUCME (Alpha-amylase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.428e-16
Identity = 38/61 (62.30%), Postives = 51/61 (83.61%), Query Frame = 2 
Query:   11 KFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPEQ 184
            KF+ L+LPK +E+WSIA+EKPK GD LIEHV E++TIE+KRKALE AL++K+   +R P+Q
Sbjct:  269 KFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEKETIEKKRKALERALQRKTKQWQRTPDQ 329

HSP 2 Score: 28.4906 bits (62), Expect = 6.428e-16
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  297 EPGTGTGREIVVSIY 341
            EPGTGTG EIVVS +
Sbjct:  333 EPGTGTGHEIVVSYH 347
[Close]
BLAST of FG535330 vs. TrEMBL
Match: D7SVC2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032922001 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 5.115e-15
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 2 
Query:   11 KFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPE 181
            KF  L+LPK DE+WSIA EKPKEGD LIEHV E++ IE+KRKALE AL +K+   ++ PE
Sbjct:  509 KFISLDLPKPDEVWSIATEKPKEGDTLIEHVIEKEIIEKKRKALERALHRKTIQWQQTPE 568

HSP 2 Score: 28.8758 bits (63), Expect = 5.115e-15
Identity = 12/15 (80.00%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  285 DANAEPGTGTGREIV 329
            D   EPGTGTGREIV
Sbjct:  569 DTQLEPGTGTGREIV 583
[Close]
BLAST of FG535330 vs. TrEMBL
Match: B9SJF1_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_0526780 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 8.638e-15
Identity = 37/61 (60.66%), Postives = 49/61 (80.33%), Query Frame = 2 
Query:    8 RKFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPE 181
            + F+ L+LPK DE+WSIA+EKPK GD LIEHV E++TIE+KRKALE  L++K+   +R PE
Sbjct:  505 KNFRHLDLPKPDEVWSIAQEKPKVGDTLIEHVMEKETIEKKRKALERVLQRKTIQWQRTPE 565

HSP 2 Score: 26.9498 bits (58), Expect = 8.638e-15
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  297 EPGTGTGREIV 329
            EPGTGTGREIV
Sbjct:  570 EPGTGTGREIV 580
[Close]
BLAST of FG535330 vs. Medicago proteins
Match: IMGA|Medtr7g013300.1 (Alpha-amylase isozyme 3D (AHRD V1 *-*- P27933); contains Interpro domain(s) IPR006589 Glycosyl hydrolase, family 13, subfamily, catalytic region chr07_pseudomolecule_IMGAG_V3.5 3414269-3399741 H EGN_Mt100125 20100825)

HSP 1 Score: 99.3673 bits (246), Expect = 7.896e-24
Identity = 50/64 (78.12%), Postives = 58/64 (90.62%), Query Frame = 2 
Query:   11 KFKPLELPKADELWSIAREKPKEGDHLIEHVFERQTIERKRKALECALRKKS---ERAPEQKIL 193
            KF+ LELPKADE+WSIAREKPKEGD LIEHVFER+TIE+KRKALE AL++K+   E+APEQKIL
Sbjct:  509 KFRSLELPKADEVWSIAREKPKEGDALIEHVFERETIEKKRKALERALQRKTIKWEKAPEQKIL 572

HSP 2 Score: 27.335 bits (59), Expect = 7.896e-24
Identity = 11/15 (73.33%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  297 EPGTGTGREIVVSIY 341
            EPGTGTGREIV   +
Sbjct:  573 EPGTGTGREIVFQAF 587
The following BLAST results are available for this feature:
BLAST of FG535330 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 2
Match NameE-valueIdentityDescription
D7SVC2_VITVI4.090e-1563.33Whole genome shotgun sequence of line PN40024, sca... [more]
B9SJF1_RICCO6.908e-1560.66Alpha-amylase, putative OS=Ricinus communis GN=RCO... [more]
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BLAST of FG535330 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 3
Match NameE-valueIdentityDescription
E5GCI0_CUCME6.428e-1662.30Alpha-amylase OS=Cucumis melo subsp. melo PE=4 SV=... [more]
D7SVC2_VITVI5.115e-1563.33Whole genome shotgun sequence of line PN40024, sca... [more]
B9SJF1_RICCO8.638e-1560.66Alpha-amylase, putative OS=Ricinus communis GN=RCO... [more]
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BLAST of FG535330 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 1
Match NameE-valueIdentityDescription
IMGA|Medtr7g013300.17.896e-2478.13Alpha-amylase isozyme 3D (AHRD V1 *-*- P27933); co... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG535330 ID=FG535330; Name=FG535330; organism=Pisum sativum; type=EST; length=450bp
TTGGCATCGAAAGTTCAAACCCTTGGAGTTGCCTAAAGCCGATGAATTAT
GGTCTATTGCTCGAGAAAAGCCAAAGGAAGGGGATCATTTAATTGAACAT
GTTTTTGAAAGACAAACAATTGAGAGAAAGAGGAAGGCATTGGAATGTGC
TCTTCGAAAGAAGTCAGAGAGAGCCCCTGAACAAAAAATACTAGGTCACG
TGTCTGCATTTTTGAAATGAAATTTTAAAATTTCTTTTGGAAGATTTATT
TTGTTAATGTGGTTCACCTCTCCCATGTAAACATGACGCAAATGCAGAGC
CAGGAACAGGAACAGGCCGAGAGATTGTGGTTAGTATTTACCAACCCAAT
AATTTGACCGAGCATGTGTTTTTACATTGTATAAGCTCCAAATGCATGTT
GAGTTGCTTAAATCTGGTGATTCAATAGAATGTAGTTTTCTCCCCCTTTT
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