FG535499

EST Overview


Name	FG535499
Unique Name	FG535499
Type	EST
Organism	Pisum sativum (pea)
Sequence length	567

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q9STA3_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v1 PE=2 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 5.155e-75
Identity = 140/155 (90.32%), Postives = 146/155 (94.19%), Query Frame = 2

Query:    2 AREKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            AREK GSTLGFDSLK LELINSVVYETLRMNPPVPLQFGRARKDFQLSS DS FNVKKGELLCG+QKLVMRDP VFDEPE FKP+RFTKEKGAELLNYLYWSNGPQ+GSP+V+NKQCAGKDIVTFT+ALIVAHLLRRYD IKGDGSSIT L KAK
Sbjct:  326 AREKGGSTLGFDSLKELELINSVVYETLRMNPPVPLQFGRARKDFQLSSYDSAFNVKKGELLCGFQKLVMRDPVVFDEPEQFKPERFTKEKGAELLNYLYWSNGPQTGSPTVSNKQCAGKDIVTFTAALIVAHLLRRYDLIKGDGSSITALQKAK 480

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q9STA2_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v2 PE=2 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 6.732e-75
Identity = 139/155 (89.68%), Postives = 146/155 (94.19%), Query Frame = 2

Query:    2 AREKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            AREK GSTLGFDSLK LELINSVVYETLRMNPPVPLQFGRARKDFQLSS DS FNVKKGELLCG+QKL+MRDP VFDEPE FKP+RFTKEKGAELLNYLYWSNGPQ+GSP+V+NKQCAGKDIVTFT+ALIVAHLLRRYD IKGDGSSIT L KAK
Sbjct:  326 AREKGGSTLGFDSLKELELINSVVYETLRMNPPVPLQFGRARKDFQLSSYDSAFNVKKGELLCGFQKLIMRDPVVFDEPEQFKPERFTKEKGAELLNYLYWSNGPQTGSPTVSNKQCAGKDIVTFTAALIVAHLLRRYDLIKGDGSSITALRKAK 480

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q4ZGM9_MEDTR (13-hydroperoxide lyase OS=Medicago truncatula GN=HPL3 PE=2 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 1.959e-74
Identity = 139/155 (89.68%), Postives = 145/155 (93.55%), Query Frame = 2

Query:    2 AREKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            AREK GSTLGFDSLK LELINSVVYETLRMNPPVPLQFGRARKDFQLSS DS FNVKKGELLCG+QKL+MRDP VFDEPE FKP+RFTKEKGAELLNYLYWSNGPQ+GSPS +NKQCAGKDIVTFT+ALIVAHLLRRYD IKGDGSSIT L KAK
Sbjct:  326 AREKGGSTLGFDSLKELELINSVVYETLRMNPPVPLQFGRARKDFQLSSYDSAFNVKKGELLCGFQKLIMRDPVVFDEPEQFKPERFTKEKGAELLNYLYWSNGPQTGSPSGSNKQCAGKDIVTFTAALIVAHLLRRYDLIKGDGSSITALRKAK 480

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q9STA1_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v3 PE=2 SV=1)

HSP 1 Score: 281.567 bits (719), Expect = 2.558e-74
Identity = 139/155 (89.68%), Postives = 145/155 (93.55%), Query Frame = 2

Query:    2 AREKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            AREK GSTLGFDSLK LELINSVVYETLRMNPPVPLQFGRARKDFQLSS D  FNVKKGELLCG+QKLVMRDP VFDEPE FKP+RFTKEKGAELLNYLYWSNGPQ+GSP+V+NKQCAGKDIVTFT+ALIVAHLLRRYD IKGDGSSIT L KAK
Sbjct:  326 AREKGGSTLGFDSLKELELINSVVYETLRMNPPVPLQFGRARKDFQLSSYDFAFNVKKGELLCGFQKLVMRDPVVFDEPEQFKPERFTKEKGAELLNYLYWSNGPQTGSPTVSNKQCAGKDIVTFTAALIVAHLLRRYDLIKGDGSSITALRKAK 480

[Close]

BLAST of FG535499 vs. TrEMBL
Match: D7TEB4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_59.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030474001 PE=4 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 3.711e-57
Identity = 103/151 (68.21%), Postives = 127/151 (84.11%), Query Frame = 2

Query:   11 KCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKA 463
            K G+ L F+S+K+LEL++SVVYETLR+NPPVPLQ+ RARKDFQLSS+DS+F +KKG+LLCG+QK+ M DP +FD+PE+F PDRFTKEKG ELLNYL+WSNGPQ+GSPS  NKQCA KD VT T+ L V H+ +RYDS+   GSSIT + KA
Sbjct:  387 KPGTNLTFESVKDLELVHSVVYETLRLNPPVPLQYARARKDFQLSSHDSVFEIKKGDLLCGFQKVAMTDPKIFDDPETFVPDRFTKEKGRELLNYLFWSNGPQTGSPSDRNKQCAAKDYVTMTAVLFVTHMFQRYDSVTASGSSITAVEKA 537

[Close]

BLAST of FG535499 vs. TrEMBL
Match: D5FV11_VITVI (Fatty acid hydroperoxide lyase OS=Vitis vinifera GN=HPLA PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 3.711e-57
Identity = 103/151 (68.21%), Postives = 127/151 (84.11%), Query Frame = 2

Query:   11 KCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKA 463
            K G+ L F+S+K+LEL++SVVYETLR+NPPVPLQ+ RARKDFQLSS+DS+F +KKG+LLCG+QK+ M DP +FD+PE+F PDRFTKEKG ELLNYL+WSNGPQ+GSPS  NKQCA KD VT T+ L V H+ +RYDS+   GSSIT + KA
Sbjct:  336 KPGTNLTFESVKDLELVHSVVYETLRLNPPVPLQYARARKDFQLSSHDSVFEIKKGDLLCGFQKVAMTDPKIFDDPETFVPDRFTKEKGRELLNYLFWSNGPQTGSPSDRNKQCAAKDYVTMTAVLFVTHMFQRYDSVTASGSSITAVEKA 486

[Close]

BLAST of FG535499 vs. TrEMBL
Match: B9STL5_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1016730 PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 2.406e-56
Identity = 106/154 (68.83%), Postives = 124/154 (80.52%), Query Frame = 2

Query:    5 REKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            REK G +L FDS+K + L+ SVVYETLR+NPPVPLQF RARKDFQLSS+DS+F +KKGE+LCGYQ LVMRD  +FD+PE+FKPDRF    G ELLNYLYWSNGPQSG+P+ +NKQC  KD  T ++ LIVAHL RRYDS  G  SSIT + KAK
Sbjct:  344 REKGGPSLNFDSVKEMPLVQSVVYETLRLNPPVPLQFARARKDFQLSSHDSVFEIKKGEILCGYQPLVMRDSEIFDDPETFKPDRF-MGSGQELLNYLYWSNGPQSGTPTASNKQCPAKDYATLSACLIVAHLFRRYDSFTGSSSSITAVEKAK 496

[Close]

BLAST of FG535499 vs. TrEMBL
Match: B9IAH7_POPTR (Cytochrome P450 fatty acid hydroperoxide lyase OS=Populus trichocarpa GN=CYP74B7 PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 2.406e-56
Identity = 104/153 (67.97%), Postives = 128/153 (83.66%), Query Frame = 2

Query:    5 REKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKA 463
            R+  G +L F+S+  + L+ SVVYETLR++PPVPLQF RARKDFQLSS+DS+F++KKGELLCGYQ LVMRD  VFD+PESF+ DRF  E+G ELLNYLYWSNGPQ+GSPS +NKQCA KD VT T +++VA+LL+RYDSI GD +SIT + KA
Sbjct:  337 RQNAGPSLSFESVMKMPLVQSVVYETLRLSPPVPLQFARARKDFQLSSHDSVFDIKKGELLCGYQPLVMRDAEVFDDPESFRADRFMGEEGRELLNYLYWSNGPQTGSPSESNKQCAAKDYVTLTGSMMVAYLLKRYDSITGDSASITAVEKA 489

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q9ZSY9_ARATH (Hydroperoxide lyase OS=Arabidopsis thaliana GN=HPL1 PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 9.141e-56
Identity = 104/150 (69.33%), Postives = 122/150 (81.33%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            GS L F ++  +EL+ SVVYETLR NPPVPLQF RARKDFQ+SS+D++F VKKGELLCGYQ LVMRD  VFDEPE FKPDR+  E G+ELLNYLYWSNGPQ+G+PS +NKQCA KDIVT T++L+VA L  RYD+I GD  SI  + KAK
Sbjct:  343 GSDLNFKTVNEMELVKSVVYETLRFNPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVADLFLRYDTITGDSGSIKAVVKAK 492

[Close]

BLAST of FG535499 vs. TrEMBL
Match: Q84V86_CITSI (Fatty acid hydroperoxide lyase OS=Citrus sinensis GN=HPL PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.559e-55
Identity = 106/155 (68.39%), Postives = 130/155 (83.87%), Query Frame = 2

Query:    5 REKCG-STLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            +EKCG S L F+S+K+LEL+ SVVYETLR+NPPVPLQF RARKDFQLSS DS++++KKGELLCGYQ LVMRD  VFD+ ESFK +RF  EKG+ELL+YLYWSNGPQ+G+P+  NKQCAGKD VT  + L VA++ +RY+SI G+ SSIT + KAK
Sbjct:  345 KEKCGTSALTFESVKSLELVQSVVYETLRLNPPVPLQFARARKDFQLSSYDSVYDIKKGELLCGYQPLVMRDSKVFDDAESFKAERFMGEKGSELLSYLYWSNGPQTGTPNDMNKQCAGKDYVTLVACLTVAYVFQRYESITGNSSSITAVEKAK 499

[Close]

BLAST of FG535499 vs. SwissProt
Match: CP74_LINUS (Allene oxide synthase, chloroplastic OS=Linum usitatissimum GN=CYP74A PE=1 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.872e-36
Identity = 74/151 (49.01%), Postives = 102/151 (67.55%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGD------GSSITL 451
            G  +   +++ + L+ SVVYETLR+ PPV LQ+G+A+KDF L S+++ + VK+GE+L GYQ    +DP +FD PE F  DRF  E G +L+ Y+ WSNGP++ +PSVANKQCAGKD V   + L V  L +RYDS   +      G+SITL
Sbjct:  379 GGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGE-GVKLMEYVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITL 528

[Close]

BLAST of FG535499 vs. SwissProt
Match: CP74A_ARATH (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana GN=CYP74A PE=1 SV=3)

HSP 1 Score: 149.058 bits (375), Expect = 1.472e-35
Identity = 68/135 (50.37%), Postives = 91/135 (67.41%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDS 421
            G  L   +++ +EL  SVVYE LR  PPV  Q+GRA+KD  + S+D+ F VK GE+L GYQ L  RDP +FD  + F P+RF  E+G +LL ++ WSNGP++ +P+V NKQCAGKD V   + L V  + RRYDS
Sbjct:  360 GGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDS 494

[Close]

BLAST of FG535499 vs. SwissProt
Match: C74A2_PARAR (Allene oxide synthase OS=Parthenium argentatum GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 149.058 bits (375), Expect = 1.472e-35
Identity = 71/145 (48.97%), Postives = 100/145 (68.97%), Query Frame = 2

Query:   35 DSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGD------GSSITL 451
            ++++ + L  SVVYE+LR+ PPVP Q+G+A+ +F + S+D+ F VKKGE+L GYQ    +DP VFD PE + PDRF  + G  LL Y++WSNGP++ SP+V NKQCAGKD V   + L V  L RRYDS + +      G+++TL
Sbjct:  322 EAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHDATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGD-GEALLKYVWWSNGPETESPTVENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTL 465

[Close]

BLAST of FG535499 vs. SwissProt
Match: C74A4_ORYSJ (Allene oxide synthase 4 OS=Oryza sativa subsp. japonica GN=CYP74A4 PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.799e-33
Identity = 70/139 (50.36%), Postives = 90/139 (64.75%), Query Frame = 2

Query:   44 KNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGA---ELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITL 451
            K + L+ SVV+E LRMNPPV  Q+GRAR+D  + S+D+ + V+KGELL GYQ L  RD  VFD    F PDRF    G+    LL ++ WSNGP++G+PS  NKQC GKD+V     L+VA L RRYD+   D   + L
Sbjct:  349 KEMPLVKSVVWEALRMNPPVEFQYGRARRDMVVESHDAAYEVRKGELLFGYQPLATRDEKVFDRAGEFVPDRFVSGAGSAARPLLEHVVWSNGPETGTPSEGNKQCPGKDMVVAVGRLMVAGLFRRYDTFAADVEELPL 487

[Close]

BLAST of FG535499 vs. SwissProt
Match: C74A1_ORYSJ (Allene oxide synthase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=CYP74A1 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 8.928e-33
Identity = 66/156 (42.31%), Postives = 99/156 (63.46%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGD------GSSITLLTKAK 466
            G  +   +L  + L+ S VYE LR+ PPV +Q+GRA++D  + S+D  + V++GE+L GYQ +  +DP VF  PE + PDRF  E GA LL ++ WSNGP++ +P++ +KQCAGKD V   + L++  L  RYDS   +      GSS+T+ +  K
Sbjct:  354 GGEVTMKALAEMPLVKSAVYEALRIEPPVAMQYGRAKRDMVVESHDYGYEVREGEMLFGYQPMATKDPRVFARPEEYVPDRFLGEDGARLLRHVVWSNGPETAAPTLHDKQCAGKDFVVLVARLLLVELFLRYDSFDVEVGTSTLGSSVTVTSLKK 509

[Close]

BLAST of FG535499 vs. SwissProt
Match: C74A2_ORYSJ (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 134.42 bits (337), Expect = 3.751e-31
Identity = 68/153 (44.44%), Postives = 96/153 (62.75%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFD-EPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSS---ITLLTKA 463
            G  +   +L+ +EL  SVV+E LR++PPV  Q+GRA+ D ++ S+D+ F +KKGE+L GYQ    RDP VF      F  DRF  E+G +LL Y+YWSNG ++ +PSV NKQC GK++V     L++  L  RYD+   +      IT +TKA
Sbjct:  317 GGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDASFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPSVDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEAGKKVVITGVTKA 469

[Close]

BLAST of FG535499 vs. SwissProt
Match: C74A3_ORYSJ (Allene oxide synthase 3 OS=Oryza sativa subsp. japonica GN=CYP74A3 PE=2 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 4.147e-30
Identity = 68/145 (46.90%), Postives = 90/145 (62.07%), Query Frame = 2

Query:    8 EKCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGA---ELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGD 433
            E  G TL   +++ + L+ SVV+E LRMNPPV  Q+G AR+D  + S+D+ + V+KGE+L GYQ L  RD  VFD    F  DRF     A    LL ++ WSNGP++ +PS  NKQC GKD+V     L+VA L RRYD+   D
Sbjct:  337 EDDGITLA--AVERMPLVKSVVWEALRMNPPVEFQYGHARRDMVVESHDAAYEVRKGEMLFGYQPLATRDEKVFDRAGEFVADRFVAGGAAGDRPLLEHVVWSNGPETRAPSEGNKQCPGKDMVVAVGRLMVAELFRRYDTFAAD 479

[Close]

BLAST of FG535499 vs. SwissProt
Match: C75AK_ARATH (Cytochrome P450 705A20 OS=Arabidopsis thaliana GN=CYP705A20 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.533e-8
Identity = 40/133 (30.08%), Postives = 66/133 (49.62%), Query Frame = 2

Query:   41 LKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTK-EKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD-SIKGD 433
            L  L  + SVV E LR++PP+PL      + FQ S     F + +   L      VMRDP  +++P+ FKP+RF + E+    L ++ + +G          + C G ++ T      +  +++ +D SIKGD
Sbjct:  353 LPKLPYLQSVVKEGLRLHPPLPLMV----RTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPERFLRQEEERRALKHIAFGSG---------RRGCPGSNLATIFIGTAIGTMVQCFDLSIKGD 472

[Close]

BLAST of FG535499 vs. SwissProt
Match: CP4G1_DROME (Cytochrome P450 4g1 OS=Drosophila melanogaster GN=Cyp4g1 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.003e-8
Identity = 38/128 (29.69%), Postives = 60/128 (46.88%), Query Frame = 2

Query:   32 FDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRY 415
            F     ++ +  V+ ETLR+ PPVPL   R   D +L+S    + V KG  +   Q  V R P ++  P  F PD F  E+   + N  Y+S  P S  P    + C G+        ++++ ++R Y
Sbjct:  400 FADTMEMKYLERVILETLRLYPPVPLIARRLDYDLKLASGP--YTVPKGTTVIVLQYCVHRRPDIYPNPTKFDPDNFLPER---MANRHYYSFIPFSAGP----RSCVGRKYAMLKLKVLLSTIVRNY 518

[Close]

BLAST of FG535499 vs. SwissProt
Match: C6A14_DROME (Probable cytochrome P450 6a14 OS=Drosophila melanogaster GN=Cyp6a14 PE=1 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 2.003e-8
Identity = 41/152 (26.97%), Postives = 69/152 (45.39%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKE--KGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            G  L +D L  +  ++ V+ ETLR +P +P       KD+Q+ ++D + +  KG L       +  DP ++ EPE F P RF  E  K    + YL + +GP         + C G       + + +  LLRR+     + + + L+   K
Sbjct:  352 GGELNYDVLAQMTYLDQVLSETLRKHPLLPHLIRETTKDYQIPNSDIVLD--KGILALIPVHNIHHDPEIYPEPEKFDPSRFDPEEVKNRHPMAYLPFGDGP---------RNCIGLRFGKIQAKIGLVSLLRRFKFSVSNRTDVPLIFSKK 492

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT4G15440.1 (| Symbols: HPL1, CYP74B2 | hydroperoxide lyase 1 | chr4:8835869-8838462 FORWARD LENGTH=384)

HSP 1 Score: 218.009 bits (554), Expect = 2.749e-57
Identity = 103/150 (68.67%), Postives = 122/150 (81.33%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDSIKGDGSSITLLTKAK 466
            GS L F ++  +EL+ SVVYETLR +PPVPLQF RARKDFQ+SS+D++F VKKGELLCGYQ LVMRD  VFDEPE FKPDR+  E G+ELLNYLYWSNGPQ+G+PS +NKQCA KDIVT T++L+VA L  RYD+I GD  SI  + KAK
Sbjct:  235 GSDLNFKTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVADLFLRYDTITGDSGSIKAVVKAK 384

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT5G42650.1 (| Symbols: AOS, CYP74A, DDE2 | allene oxide synthase | chr5:17097803-17099359 REVERSE LENGTH=518)

HSP 1 Score: 149.058 bits (375), Expect = 1.568e-36
Identity = 68/135 (50.37%), Postives = 91/135 (67.41%), Query Frame = 2

Query:   17 GSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYDS 421
            G  L   +++ +EL  SVVYE LR  PPV  Q+GRA+KD  + S+D+ F VK GE+L GYQ L  RDP +FD  + F P+RF  E+G +LL ++ WSNGP++ +P+V NKQCAGKD V   + L V  + RRYDS
Sbjct:  360 GGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDS 494

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT3G20110.1 (| Symbols: CYP705A20 | cytochrome P450, family 705, subfamily A, polypeptide 20 | chr3:7021495-7023113 FORWARD LENGTH=510)

HSP 1 Score: 59.3066 bits (142), Expect = 1.634e-9
Identity = 40/133 (30.08%), Postives = 66/133 (49.62%), Query Frame = 2

Query:   41 LKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTK-EKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD-SIKGD 433
            L  L  + SVV E LR++PP+PL      + FQ S     F + +   L      VMRDP  +++P+ FKP+RF + E+    L ++ + +G          + C G ++ T      +  +++ +D SIKGD
Sbjct:  353 LPKLPYLQSVVKEGLRLHPPLPLMV----RTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPERFLRQEEERRALKHIAFGSG---------RRGCPGSNLATIFIGTAIGTMVQCFDLSIKGD 472

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT3G20120.2 (| Symbols: CYP705A21 | cytochrome P450, family 705, subfamily A, polypeptide 21 | chr3:7024576-7025789 FORWARD LENGTH=378)

HSP 1 Score: 57.3806 bits (137), Expect = 6.211e-9
Identity = 42/140 (30.00%), Postives = 68/140 (48.57%), Query Frame = 2

Query:   41 LKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRF--------TKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD-SIKGD 433
            L  L  + +VV E LR++PP+PL      + FQ       F V +   L G   ++MRDP+V+++PE FKP+RF         +E+  + L Y+ + +G          + C G  +        V  +++ +D SIKGD
Sbjct:  219 LPKLPYLQAVVKEGLRLHPPLPLFV----RTFQEGCKIGGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSRSTQEEERREQALKYIPFGSG---------RRGCPGSSLGYIFVGTAVGMMVQCFDWSIKGD 345

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT3G20120.1 (| Symbols: CYP705A21 | cytochrome P450, family 705, subfamily A, polypeptide 21 | chr3:7024576-7025789 FORWARD LENGTH=378)

HSP 1 Score: 57.3806 bits (137), Expect = 6.211e-9
Identity = 42/140 (30.00%), Postives = 68/140 (48.57%), Query Frame = 2

Query:   41 LKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRF--------TKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD-SIKGD 433
            L  L  + +VV E LR++PP+PL      + FQ       F V +   L G   ++MRDP+V+++PE FKP+RF         +E+  + L Y+ + +G          + C G  +        V  +++ +D SIKGD
Sbjct:  219 LPKLPYLQAVVKEGLRLHPPLPLFV----RTFQEGCKIGGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSRSTQEEERREQALKYIPFGSG---------RRGCPGSSLGYIFVGTAVGMMVQCFDWSIKGD 345

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT1G11680.1 (| Symbols: CYP51G1, EMB1738, CYP51A2, CYP51 | CYTOCHROME P450 51G1 | chr1:3938925-3940585 FORWARD LENGTH=488)

HSP 1 Score: 57.3806 bits (137), Expect = 6.211e-9
Identity = 32/141 (22.70%), Postives = 67/141 (47.52%), Query Frame = 2

Query:   11 KCGSTLGFDSLKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSND-SLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFT----KEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD 418
            K G  +  D L  ++++   + E LR++PP+ +    +  DF +++ D   +++ KG ++        R P +F +P+++ P+RF+    ++K A   +Y+ +  G            C G+         I +HLLR ++
Sbjct:  324 KHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGRHG---------CLGEPFAYLQIKAIWSHLLRNFE 455

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 55.4546 bits (132), Expect = 2.360e-8
Identity = 35/103 (33.98%), Postives = 56/103 (54.37%), Query Frame = 2

Query:    2 AREKCGSTLGFD------SLKNLELINSVVYETLRMNPPVPLQFGR-ARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELL 289
            AR++  + +G D       L  L  + ++V ETLR++P  PL     A +D ++ S D    + +G  L      + RDP  +D+P+SFKP+RF KE+ A+ L
Sbjct:  332 ARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYD----MPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEEAQKL 430

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 55.4546 bits (132), Expect = 2.360e-8
Identity = 39/105 (37.14%), Postives = 53/105 (50.48%), Query Frame = 2

Query:    2 AREKCGSTLGFDSLK------NLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSL---FNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEKGAELL 289
            AR +    +G D L       NL  + S+V ETLRM P VPL          LSS D     +++  G ++      + RDP V+++PE FKP+RF KE  AE L
Sbjct:  323 ARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAVPLLLPH------LSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEAEKL 421

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT2G23220.1 (| Symbols: CYP81D6 | cytochrome P450, family 81, subfamily D, polypeptide 6 | chr2:9884550-9886752 FORWARD LENGTH=515)

HSP 1 Score: 54.299 bits (129), Expect = 5.258e-8
Identity = 31/97 (31.96%), Postives = 53/97 (54.64%), Query Frame = 2

Query:    2 AREKCGSTLGFDSL------KNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRFTKEK 274
            AR +  + +GFD L      KNL  +  +V ETLR++P  P     +  +  + +    ++V +G +L      + RDP+++++PE FKP+RF  EK
Sbjct:  341 ARTEIDTKIGFDRLMDEADTKNLPYLQWIVLETLRLHPAAPTNVPHSTSEDCMLAG---YDVPRGSMLLVNIWSMHRDPSIWEDPEMFKPERFKNEK 434

[Close]

BLAST of FG535499 vs. TAIR peptide
Match: AT4G15330.1 (| Symbols: CYP705A1 | cytochrome P450, family 705, subfamily A, polypeptide 1 | chr4:8751523-8753134 REVERSE LENGTH=513)

HSP 1 Score: 53.9138 bits (128), Expect = 6.867e-8
Identity = 39/138 (28.26%), Postives = 65/138 (47.10%), Query Frame = 2

Query:   41 LKNLELINSVVYETLRMNPPVPLQFGRARKDFQLSSNDSLFNVKKGELLCGYQKLVMRDPAVFDEPESFKPDRF------TKEKGAELLNYLYWSNGPQSGSPSVANKQCAGKDIVTFTSALIVAHLLRRYD-SIKGD 433
            L NL  + +VV E LR++PP PL      ++FQ       F V K   L      +MRDP  + +P+ FKP+RF       ++K  ++LN+L + +G          + C G ++        +  +++ +D  I GD
Sbjct:  352 LPNLPYLQAVVKEGLRLHPPTPLVV----REFQEGCEIGGFFVPKNTTLIVNSYAMMRDPDSWQDPDEFKPERFLASLSREEDKKEKILNFLPFGSG---------RRMCPGSNLGYIFVGTAIGMMVQCFDWEINGD 476

The following BLAST results are available for this feature:

BLAST of FG535499 vs. TrEMBL
BLAST of FG535499 vs. SwissProt
BLAST of FG535499 vs. TAIR peptide

BLAST of FG535499 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9STA3_MEDSA	5.155e-75	90.32	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
Q9STA2_MEDSA	6.732e-75	89.68	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
Q4ZGM9_MEDTR	1.959e-74	89.68	13-hydroperoxide lyase OS=Medicago truncatula GN=H...	[more]
Q9STA1_MEDSA	2.558e-74	89.68	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
D7TEB4_VITVI	3.711e-57	68.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
D5FV11_VITVI	3.711e-57	68.21	Fatty acid hydroperoxide lyase OS=Vitis vinifera G...	[more]
B9STL5_RICCO	2.406e-56	68.83	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
B9IAH7_POPTR	2.406e-56	67.97	Cytochrome P450 fatty acid hydroperoxide lyase OS=...	[more]
Q9ZSY9_ARATH	9.141e-56	69.33	Hydroperoxide lyase OS=Arabidopsis thaliana GN=HPL...	[more]
Q84V86_CITSI	1.559e-55	68.39	Fatty acid hydroperoxide lyase OS=Citrus sinensis ...	[more]

BLAST of FG535499 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CP74_LINUS	3.872e-36	49.01	Allene oxide synthase, chloroplastic OS=Linum usit...	[more]
CP74A_ARATH	1.472e-35	50.37	Allene oxide synthase, chloroplastic OS=Arabidopsi...	[more]
C74A2_PARAR	1.472e-35	48.97	Allene oxide synthase OS=Parthenium argentatum GN=...	[more]
C74A4_ORYSJ	1.799e-33	50.36	Allene oxide synthase 4 OS=Oryza sativa subsp. jap...	[more]
C74A1_ORYSJ	8.928e-33	42.31	Allene oxide synthase 1, chloroplastic OS=Oryza sa...	[more]
C74A2_ORYSJ	3.751e-31	44.44	Allene oxide synthase 2 OS=Oryza sativa subsp. jap...	[more]
C74A3_ORYSJ	4.147e-30	46.90	Allene oxide synthase 3 OS=Oryza sativa subsp. jap...	[more]
C75AK_ARATH	1.533e-8	30.08	Cytochrome P450 705A20 OS=Arabidopsis thaliana GN=...	[more]
CP4G1_DROME	2.003e-8	29.69	Cytochrome P450 4g1 OS=Drosophila melanogaster GN=...	[more]
C6A14_DROME	2.003e-8	26.97	Probable cytochrome P450 6a14 OS=Drosophila melano...	[more]

BLAST of FG535499 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G15440.1	2.749e-57	68.67	\| Symbols: HPL1, CYP74B2 \| hydroperoxide lyase 1 \|...	[more]
AT5G42650.1	1.568e-36	50.37	\| Symbols: AOS, CYP74A, DDE2 \| allene oxide syntha...	[more]
AT3G20110.1	1.634e-9	30.08	\| Symbols: CYP705A20 \| cytochrome P450, family 705...	[more]
AT3G20120.2	6.211e-9	30.00	\| Symbols: CYP705A21 \| cytochrome P450, family 705...	[more]
AT3G20120.1	6.211e-9	30.00	\| Symbols: CYP705A21 \| cytochrome P450, family 705...	[more]
AT1G11680.1	6.211e-9	22.70	\| Symbols: CYP51G1, EMB1738, CYP51A2, CYP51 \| CYTO...	[more]
AT5G36220.1	2.360e-8	33.98	\| Symbols: CYP81D1, CYP91A1 \| cytochrome p450 81d1...	[more]
AT4G37330.1	2.360e-8	37.14	\| Symbols: CYP81D4 \| cytochrome P450, family 81, s...	[more]
AT2G23220.1	5.258e-8	31.96	\| Symbols: CYP81D6 \| cytochrome P450, family 81, s...	[more]
AT4G15330.1	6.867e-8	28.26	\| Symbols: CYP705A1 \| cytochrome P450, family 705,...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 7..140 score: 3.9
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 11..138 score: 3.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 11..139 score: 2.1
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 6..146 score: 3.2
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 17..33 score: 1.6e-12coord: 54..68 score: 1.6e-12coord: 70..88 score: 1.6e-12coord: 117..135 score: 1.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG535499 ID=FG535499; Name=FG535499; organism=Pisum sativum; type=EST; length=567bp
AGCAAGAGAGAAATGCGGTTCAACTCTCGGGTTTGACTCGTTGAAAAACT
TGGAGCTAATTAATTCAGTAGTTTACGAAACCCTTCGGATGAACCCCCCG
GTTCCTCTTCAGTTCGGTCGAGCCAGAAAGGATTTTCAGCTCAGTTCAAA
TGACTCACTATTCAATGTGAAAAAGGGCGAGTTATTATGTGGTTATCAGA
AGCTTGTAATGAGAGACCCGGCTGTGTTTGACGAACCAGAGAGTTTTAAA
CCGGATCGGTTCACTAAAGAGAAAGGAGCCGAGTTGTTGAACTATTTGTA
TTGGTCTAATGGACCGCAATCCGGTTCGCCAAGCGTGGCCAATAAACAGT
GTGCAGGTAAGGACATTGTGACCTTCACTTCTGCCTTAATTGTGGCACAC
TTGCTTCGTAGGTATGATTCAATTAAAGGGGATGGTAGTTCCATAACTCT
CTTAACAAAGGCCAAATGATGAGTTAGAGTTTGGTTTTCAATGAACATGA
ATATTTGTTTATGATTTTATCAGTGACAGATTATGTATATTTTTCTCTAT
AAAAGAGGACTTGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002403	Cyt_P450_E_grp-IV

Search form