FG537255

EST Overview


Name	FG537255
Unique Name	FG537255
Type	EST
Organism	Pisum sativum (pea)
Sequence length	416

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG537255 vs. TrEMBL
Match: A9RSV5_PHYPA (DNA-directed RNA polymerase OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_177840 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.902e-13
Identity = 39/68 (57.35%), Postives = 52/68 (76.47%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLEP-PVLRRGAVPILS 209
            S LMLASFE +TD LFDAA+ G+ D I+GVS+CIIMG P+ IGTG+ +++Q++E  P L  G  P+LS
Sbjct: 1330 SVLMLASFEKTTDHLFDAAIHGRVDKIEGVSECIIMGIPMPIGTGLFKIRQKVEKLPKLSYGPTPLLS 1397

[Close]

BLAST of FG537255 vs. TrEMBL
Match: B9T0Q9_RICCO (DNA-directed RNA polymerase OS=Ricinus communis GN=RCOM_1440440 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.096e-13
Identity = 39/69 (56.52%), Postives = 54/69 (78.26%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLE-PPVLRRGAVPILS 209
            +S LMLASFE + D L+ AA+ G+ D I+GVS+CIIMG P+ IGTG+++VQQR++ PPVL  G+  I+S
Sbjct: 1315 KSVLMLASFEKTADHLYHAAVFGRDDKIEGVSECIIMGIPMQIGTGILKVQQRVDPPPVLNYGSDSIIS 1383

[Close]

BLAST of FG537255 vs. TrEMBL
Match: D7SKP8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025079001 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.314e-12
Identity = 37/69 (53.62%), Postives = 53/69 (76.81%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLEP-PVLRRGAVPILS 209
            +S LMLASFE + D LF+A++ G+ D I+GVS+CIIMG P+ +GTG+++V+QRL+  P L  G  PI+S
Sbjct: 1751 KSVLMLASFEKTADHLFNASVSGRDDKIEGVSECIIMGIPMQLGTGILKVRQRLQQVPELSYGLDPIIS 1819

[Close]

BLAST of FG537255 vs. TrEMBL
Match: C1MN08_MICPS (DNA-directed RNA polymerase OS=Micromonas pusilla CCMP1545 GN=MICPUCDRAFT_46863 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.612e-12
Identity = 35/52 (67.31%), Postives = 43/52 (82.69%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            S LMLASFE +TD LFDAALRG+ D IDGVS+CIIMG P+ +GTGM +++ R
Sbjct: 1488 SVLMLASFEKTTDHLFDAALRGRVDTIDGVSECIIMGIPMPVGTGMFKLRHR 1539

[Close]

BLAST of FG537255 vs. TrEMBL
Match: C1E5P6_9CHLO (DNA-directed RNA polymerase OS=Micromonas sp. RCC299 GN=MICPUN_82079 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.612e-12
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            S LMLASFE +TD LFDAAL G+ D IDGVS+CIIMG P+ +GTGM ++Q R
Sbjct: 1334 SVLMLASFEKTTDHLFDAALHGRTDTIDGVSECIIMGIPMPVGTGMFKLQHR 1385

[Close]

BLAST of FG537255 vs. TrEMBL
Match: Q01DV6_OSTTA (DNA-directed RNA polymerase OS=Ostreococcus tauri GN=Ot02g06220 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.639e-11
Identity = 36/52 (69.23%), Postives = 41/52 (78.85%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            S LMLASFE +TD LFDAAL G+ D IDGVS+CIIMG P+ IGTGM  +Q R
Sbjct: 1322 SVLMLASFEKTTDHLFDAALHGRTDYIDGVSECIIMGIPMPIGTGMFRLQHR 1373

[Close]

BLAST of FG537255 vs. TrEMBL
Match: A4RTE2_OSTLU (DNA-directed RNA polymerase OS=Ostreococcus lucimarinus (strain CCE9901) GN=OSTLU_24022 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.639e-11
Identity = 36/52 (69.23%), Postives = 41/52 (78.85%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            S LMLASFE +TD LFDAAL G+ D IDGVS+CIIMG P+ IGTGM  +Q R
Sbjct: 1323 SVLMLASFEKTTDHLFDAALHGRTDYIDGVSECIIMGIPMPIGTGMFRLQHR 1374

[Close]

BLAST of FG537255 vs. TrEMBL
Match: B9GIK9_POPTR (DNA-directed RNA polymerase OS=Populus trichocarpa GN=POPTRDRAFT_1068975 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.141e-11
Identity = 32/53 (60.38%), Postives = 46/53 (86.79%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            +S LMLASFE ++D LF+A+++GK D I+GVS+CIIMG P+ IGTG++++QQR
Sbjct: 1342 KSVLMLASFEKTSDHLFNASVKGKDDKIEGVSECIIMGIPVAIGTGVLKIQQR 1394

[Close]

BLAST of FG537255 vs. TrEMBL
Match: A7SJ34_NEMVE (DNA-directed RNA polymerase OS=Nematostella vectensis GN=v1g190300 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.230e-11
Identity = 33/49 (67.35%), Postives = 41/49 (83.67%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEV 155
            S LMLASFE + D LF+AAL G+ DPIDGVS+CIIMG P++IGTGM ++
Sbjct: 1317 SVLMLASFERTADHLFEAALHGRKDPIDGVSECIIMGMPMNIGTGMFKL 1365

[Close]

BLAST of FG537255 vs. TrEMBL
Match: D7MT56_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496142 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.137e-11
Identity = 36/69 (52.17%), Postives = 51/69 (73.91%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLEP-PVLRRGAVPILS 209
            +S LM ASFE + D LF AA+ GK D I+GV++C+IMG P+ +GTG+++V QR +  P L+ GA PI+S
Sbjct: 1308 KSVLMQASFERTGDHLFSAAISGKVDNIEGVTECVIMGIPMKLGTGILKVLQRTDDLPKLKYGADPIIS 1376

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_HUMAN (DNA-directed RNA polymerase III subunit RPC1 OS=Homo sapiens GN=POLR3A PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 2.433e-9
Identity = 29/54 (53.70%), Postives = 40/54 (74.07%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLE 170
            S LMLASFE + D LFDAA  G+ D + GVS+CIIMG P++IGTG+ ++  + +
Sbjct: 1314 SVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKAD 1367

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_CHICK (DNA-directed RNA polymerase III subunit RPC1 OS=Gallus gallus GN=POLR3A PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.433e-9
Identity = 29/54 (53.70%), Postives = 40/54 (74.07%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLE 170
            S LMLASFE + D LFDAA  G+ D + GVS+CIIMG P++IGTG+ ++  + +
Sbjct: 1314 SVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKAD 1367

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_BOVIN (DNA-directed RNA polymerase III subunit RPC1 OS=Bos taurus GN=POLR3A PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.178e-9
Identity = 29/49 (59.18%), Postives = 38/49 (77.55%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEV 155
            S LMLASFE + D LFDAA  G+ D + GVS+CIIMG P++IGTG+ ++
Sbjct: 1314 SVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKL 1362

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_DICDI (DNA-directed RNA polymerase III subunit rpc1 OS=Dictyostelium discoideum GN=polr3a PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.208e-8
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQR 164
            S LMLASFE +TD LFDAA+  + D I GVS+CIIMG  I +GTG+ ++ ++
Sbjct: 1384 SVLMLASFEKTTDHLFDAAVHHRQDDIVGVSECIIMGVVIPLGTGLFKLLRK 1435

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_YEAST (DNA-directed RNA polymerase III subunit RPC1 OS=Saccharomyces cerevisiae GN=RPO31 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.577e-8
Identity = 28/49 (57.14%), Postives = 36/49 (73.47%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEV 155
            S L LASFE +TD LFDAA   K D ++GVS+CII+G+ + IGTG  +V
Sbjct: 1382 SVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQTMSIGTGSFKV 1430

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_DEBHA (DNA-directed RNA polymerase III subunit RPC1 OS=Debaryomyces hansenii GN=RPC1 PE=3 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 2.060e-8
Identity = 27/49 (55.10%), Postives = 37/49 (75.51%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEV 155
            S L LASFE +TD LFDA+   K D I+GVS+CII+G+ ++IGTG  ++
Sbjct: 1381 SVLQLASFEKTTDHLFDASFYMKNDKIEGVSECIILGQTMNIGTGAFKL 1429

[Close]

BLAST of FG537255 vs. SwissProt
Match: RPC1_SCHPO (DNA-directed RNA polymerase III subunit rpc1 OS=Schizosaccharomyces pombe GN=rpc1 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 3.884e-7
Identity = 26/54 (48.15%), Postives = 38/54 (70.37%), Query Frame = 3

Query:    9 SALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLE 170
            S L LASFE +TD LF+AA R   D I+G+S+CI++G+   IGT + ++ +R E
Sbjct: 1328 SVLALASFEKTTDHLFNAAARFAKDSIEGISECIVLGKLAPIGTNVFQLIRRTE 1381

[Close]

BLAST of FG537255 vs. TAIR peptide
Match: AT5G60040.2 (| Symbols: NRPC1 | nuclear RNA polymerase C1 | chr5:24173590-24183269 FORWARD LENGTH=1391)

HSP 1 Score: 67.0106 bits (162), Expect = 4.053e-12
Identity = 35/69 (50.72%), Postives = 49/69 (71.01%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLEP-PVLRRGAVPILS 209
            +S LM ASFE + D LF AA  GK D I+GV++C+IMG P+ +GTG+++V QR +  P L+ G  PI+S
Sbjct: 1323 KSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS 1391

[Close]

BLAST of FG537255 vs. TAIR peptide
Match: AT5G60040.1 (| Symbols: NRPC1 | nuclear RNA polymerase C1 | chr5:24173590-24183269 FORWARD LENGTH=1376)

HSP 1 Score: 67.0106 bits (162), Expect = 4.053e-12
Identity = 35/69 (50.72%), Postives = 49/69 (71.01%), Query Frame = 3

Query:    6 RSALMLASFESSTDCLFDAALRGKGDPIDGVSDCIIMGEPIHIGTGMIEVQQRLEP-PVLRRGAVPILS 209
            +S LM ASFE + D LF AA  GK D I+GV++C+IMG P+ +GTG+++V QR +  P L+ G  PI+S
Sbjct: 1308 KSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS 1376

The following BLAST results are available for this feature:

BLAST of FG537255 vs. TrEMBL
BLAST of FG537255 vs. SwissProt
BLAST of FG537255 vs. TAIR peptide

BLAST of FG537255 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A9RSV5_PHYPA	3.902e-13	57.35	DNA-directed RNA polymerase OS=Physcomitrella pate...	[more]
B9T0Q9_RICCO	5.096e-13	56.52	DNA-directed RNA polymerase OS=Ricinus communis GN...	[more]
D7SKP8_VITVI	4.314e-12	53.62	Whole genome shotgun sequence of line PN40024, sca...	[more]
C1MN08_MICPS	9.612e-12	67.31	DNA-directed RNA polymerase OS=Micromonas pusilla ...	[more]
C1E5P6_9CHLO	9.612e-12	67.31	DNA-directed RNA polymerase OS=Micromonas sp. RCC2...	[more]
Q01DV6_OSTTA	1.639e-11	69.23	DNA-directed RNA polymerase OS=Ostreococcus tauri ...	[more]
A4RTE2_OSTLU	1.639e-11	69.23	DNA-directed RNA polymerase OS=Ostreococcus lucima...	[more]
B9GIK9_POPTR	2.141e-11	60.38	DNA-directed RNA polymerase OS=Populus trichocarpa...	[more]
A7SJ34_NEMVE	6.230e-11	67.35	DNA-directed RNA polymerase OS=Nematostella vecten...	[more]
D7MT56_ARALY	8.137e-11	52.17	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of FG537255 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
RPC1_HUMAN	2.433e-9	53.70	DNA-directed RNA polymerase III subunit RPC1 OS=Ho...	[more]
RPC1_CHICK	2.433e-9	53.70	DNA-directed RNA polymerase III subunit RPC1 OS=Ga...	[more]
RPC1_BOVIN	3.178e-9	59.18	DNA-directed RNA polymerase III subunit RPC1 OS=Bo...	[more]
RPC1_DICDI	1.208e-8	55.77	DNA-directed RNA polymerase III subunit rpc1 OS=Di...	[more]
RPC1_YEAST	1.577e-8	57.14	DNA-directed RNA polymerase III subunit RPC1 OS=Sa...	[more]
RPC1_DEBHA	2.060e-8	55.10	DNA-directed RNA polymerase III subunit RPC1 OS=De...	[more]
RPC1_SCHPO	3.884e-7	48.15	DNA-directed RNA polymerase III subunit rpc1 OS=Sc...	[more]

BLAST of FG537255 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G60040.2	4.053e-12	50.72	\| Symbols: NRPC1 \| nuclear RNA polymerase C1 \| chr...	[more]
AT5G60040.1	4.053e-12	50.72	\| Symbols: NRPC1 \| nuclear RNA polymerase C1 \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 50..62 score
None	No IPR available	PANTHER	PTHR19376	DNA-DIRECTED RNA POLYMERASE	coord: 3..56 score: 1.4
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 2..58 score: 8
IPR015700	DNA-directed RNA polymerase III largest subunit	PANTHER	PTHR19376:SF15	DNA-DIRECTED RNA POLYMERASE III LARGEST SUBUNIT	coord: 3..56 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG537255 ID=FG537255; Name=FG537255; organism=Pisum sativum; type=EST; length=416bp
TGGGCAGAAGTGCACTGATGCTTGCATCATTTGAGAGTTCAACGGACTGT
CTTTTCGACGCAGCCTTACGTGGGAAGGGTGACCCAATTGATGGAGTGAG
TGATTGCATAATCATGGGTGAACCGATTCATATAGGCACCGGAATGATCG
AGGTCCAACAAAGGTTGGAACCTCCTGTGTTGCGTCGGGGAGCTGTTCCT
ATTCTATCTGGAGTTTGAAGTTGGATGGTATTAGCAGCAAGAGTGTGATT
CATACAGCAAGGATTGCATTGGATGTTTTGGATTTGAGATTGTTAAATTT
ACTTAGATCTGACTTTATTTGCCACCTATGCCGTATTCGGTTTTTGTTTC
AAAAGAATTTGATCATTGTTTTTCACCTCGAGTACGGTTTCATACATGTC
ACTCTACATTTATTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0008270	zinc ion binding
GO:0032549	ribonucleoside binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription

Vocabulary: **INTERPRO**
Term	Definition
IPR015700	RPC1

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