FG537574

EST Overview


Name	FG537574
Unique Name	FG537574
Type	EST
Organism	Pisum sativum (pea)
Sequence length	563

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG537574 vs. TrEMBL
Match: C6TMB0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.105e-42
Identity = 67/85 (78.82%), Postives = 73/85 (85.88%), Query Frame = 2

Query:   77 ESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIGAIACPKT 331
            ESKCVGIC NTCKFPTQ+FFKDH+GVPLLMEPNF DYS QF+FGVLPPL  DD +VK+PCLEACP ASQRR  A+NI   ACPKT
Sbjct:  182 ESKCVGICTNTCKFPTQSFFKDHMGVPLLMEPNFGDYSCQFKFGVLPPL--DDTIVKDPCLEACPNASQRRTVARNIDITACPKT 264

HSP 2 Score: 52.373 bits (124), Expect = 1.105e-42
Identity = 22/27 (81.48%), Postives = 26/27 (96.30%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            +NSVDLPDG SC+SGVYVERCKYLE++
Sbjct:  157 LNSVDLPDGISCSSGVYVERCKYLEES 183

[Close]

BLAST of FG537574 vs. TrEMBL
Match: B9RNC7_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1346590 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 2.381e-39
Identity = 60/84 (71.43%), Postives = 71/84 (84.52%), Query Frame = 2

Query:   77 ESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIGAIACPK 328
            ESKCVGIC+NTCK PTQ FFKD++GVPLLMEPNF DYS QF+FGVLPP PEDD+ +KEPCLEACPIAS+R++   ++    CPK
Sbjct:  201 ESKCVGICVNTCKLPTQAFFKDYMGVPLLMEPNFTDYSCQFKFGVLPPQPEDDSTLKEPCLEACPIASRRQV---SLNIAHCPK 281

HSP 2 Score: 51.9878 bits (123), Expect = 2.381e-39
Identity = 21/27 (77.78%), Postives = 26/27 (96.30%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VNS+DLPDG+SC SGV+VERCKYLE++
Sbjct:  176 VNSIDLPDGSSCESGVFVERCKYLEES 202

[Close]

BLAST of FG537574 vs. TrEMBL
Match: B9GSU3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_409549 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.380e-38
Identity = 58/71 (81.69%), Postives = 65/71 (91.55%), Query Frame = 2

Query:   77 ESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRR 289
            ESKCVGIC+NTCK PTQTFFKD++G+PLLMEPNF DYS QF+FGVLPPLPEDD  +KEPCLE CPIAS+RR
Sbjct:  130 ESKCVGICVNTCKLPTQTFFKDYMGIPLLMEPNFNDYSCQFKFGVLPPLPEDDGTLKEPCLEVCPIASKRR 200

HSP 2 Score: 48.1358 bits (113), Expect = 3.380e-38
Identity = 21/27 (77.78%), Postives = 25/27 (92.59%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VNSVDLPDG+S  SGV+VERCKYLE++
Sbjct:  105 VNSVDLPDGSSWESGVFVERCKYLEES 131

[Close]

BLAST of FG537574 vs. TrEMBL
Match: D7U2J7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028138001 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 5.116e-36
Identity = 56/87 (64.37%), Postives = 69/87 (79.31%), Query Frame = 2

Query:   77 ESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIG---AIACPK 328
            ESKCVGICINTCK PTQTFFKD++GVPL MEP+F +YS QF FGVLPP PE+D+ +KEPCLE CP A++R+   +N+     + CPK
Sbjct:  174 ESKCVGICINTCKLPTQTFFKDYMGVPLAMEPDFTNYSCQFSFGVLPPRPEEDSTLKEPCLEICPNATRRKEINRNMDNKELVQCPK 260

HSP 2 Score: 50.8322 bits (120), Expect = 5.116e-36
Identity = 21/27 (77.78%), Postives = 27/27 (100.00%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VNSV+LPDG+SC+SGV+VERCKYLE++
Sbjct:  149 VNSVNLPDGSSCSSGVFVERCKYLEES 175

[Close]

BLAST of FG537574 vs. TrEMBL
Match: C6SY57_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.515e-34
Identity = 55/85 (64.71%), Postives = 61/85 (71.76%), Query Frame = 2

Query:   77 ESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIGAIACPKT 331
            ESKCVGIC +TCKFPTQTFFKDH+GVPLLMEPNFADYS QF+FGVLP  P DD +VK   L +           +NI   ACPKT
Sbjct:  183 ESKCVGICTHTCKFPTQTFFKDHMGVPLLMEPNFADYSCQFKFGVLP--PRDDTIVKGTLLGSMSKCKTTTNGCQNIDITACPKT 265

HSP 2 Score: 55.8398 bits (133), Expect = 3.515e-34
Identity = 24/27 (88.89%), Postives = 27/27 (100.00%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VNSVDLPDGTSC+SGVYVERCKYLE++
Sbjct:  158 VNSVDLPDGTSCSSGVYVERCKYLEES 184

[Close]

BLAST of FG537574 vs. TrEMBL
Match: D7M463_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_912030 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.268e-30
Identity = 53/78 (67.95%), Postives = 61/78 (78.21%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRM 292
            K Q   ESKCVG+CINTCK PTQTFFKD++GVPL+MEPNF DYS QF+FGV P  PEDD  V EPC E C IA +R++
Sbjct:  180 KCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAP--PEDDGNVNEPCFETCSIAGRRKL 255

HSP 2 Score: 40.4318 bits (93), Expect = 1.268e-30
Identity = 16/27 (59.26%), Postives = 24/27 (88.89%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VN +DLP+G S +SGV+VE+C+YLE++
Sbjct:  161 VNIIDLPNGESWDSGVFVEKCQYLEES 187

[Close]

BLAST of FG537574 vs. TrEMBL
Match: Q8W4C4_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana GN=At4g01995 PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.269e-30
Identity = 53/78 (67.95%), Postives = 61/78 (78.21%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRM 292
            K Q   ESKCVG+CINTCK PTQTFFKD++GVPL+MEPNF DYS QF+FGV P  PEDD  V EPC E C IA +R++
Sbjct:  175 KCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAP--PEDDGNVNEPCFETCSIAGRRKL 250

HSP 2 Score: 40.4318 bits (93), Expect = 1.269e-30
Identity = 16/27 (59.26%), Postives = 24/27 (88.89%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VN +DLP+G S +SGV+VE+C+YLE++
Sbjct:  156 VNIIDLPNGESWDSGVFVEKCQYLEES 182

[Close]

BLAST of FG537574 vs. TrEMBL
Match: A9NQE7_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 9.998e-27
Identity = 46/90 (51.11%), Postives = 57/90 (63.33%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIGAIACPK 328
            K Q    SKC GICI+TCK PTQTF K+++G+PLLMEPNF D+S QF+FGV      DD  +  PCLE CP   +R+     +    CPK
Sbjct:  186 KCQYLEASKCAGICIHTCKLPTQTFIKEYMGIPLLMEPNFNDFSCQFKFGVEALPTCDDKSLHVPCLEICPNDVKRKGYQNRLDVQQCPK 275

HSP 2 Score: 47.3654 bits (111), Expect = 9.998e-27
Identity = 19/25 (76.00%), Postives = 23/25 (92.00%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLE 77
            VN +DLPDG+SCNSGV VE+C+YLE
Sbjct:  167 VNCIDLPDGSSCNSGVLVEKCQYLE 191

[Close]

BLAST of FG537574 vs. TrEMBL
Match: C5YZG8_SORBI (Putative uncharacterized protein Sb09g002570 OS=Sorghum bicolor GN=Sb09g002570 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.547e-23
Identity = 53/92 (57.61%), Postives = 64/92 (69.57%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIG--AIACPK 328
            K +   ESKC+GICINTCK PTQTFFKDH+GV L MEPNF DYS QF FGV PP  + D  +KEPCL+ C  A +RR   +N     ++CP+
Sbjct:  185 KCKYLEESKCLGICINTCKLPTQTFFKDHMGVDLYMEPNFEDYSCQFNFGVPPPPLDTDKALKEPCLDICTNARRRRELGRNSSPDELSCPQ 276

[Close]

BLAST of FG537574 vs. TrEMBL
Match: C0PPG3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.547e-23
Identity = 53/92 (57.61%), Postives = 64/92 (69.57%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRMAAKNIG--AIACPK 328
            K +   ESKC+GICINTCK PTQTFFKDH+GV L MEPNF DYS QF FGV PP  + D  +KEPCL+ C  A +RR   +N     ++CP+
Sbjct:  185 KCKYLEESKCLGICINTCKLPTQTFFKDHMGVDLYMEPNFEDYSCQFNFGVPPPPLDTDKALKEPCLDICTNARRRRELGRNSSPDELSCPQ 276

[Close]

BLAST of FG537574 vs. TAIR peptide
Match: AT4G01995.1 (| Symbols: | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr4:873075-874619 FORWARD LENGTH=258)

HSP 1 Score: 117.087 bits (292), Expect = 1.003e-32
Identity = 53/78 (67.95%), Postives = 61/78 (78.21%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEACPIASQRRM 292
            K Q   ESKCVG+CINTCK PTQTFFKD++GVPL+MEPNF DYS QF+FGV P  PEDD  V EPC E C IA +R++
Sbjct:  175 KCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAP--PEDDGNVNEPCFETCSIAGRRKL 250

HSP 2 Score: 40.4318 bits (93), Expect = 1.003e-32
Identity = 16/27 (59.26%), Postives = 24/27 (88.89%), Query Frame = 3

Query:    3 VNSVDLPDGTSCNSGVYVERCKYLEKA 83
            VN +DLP+G S +SGV+VE+C+YLE++
Sbjct:  156 VNIIDLPNGESWDSGVFVEKCQYLEES 182

[Close]

BLAST of FG537574 vs. TAIR peptide
Match: AT1G03055.1 (| Symbols: | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). | chr1:710102-711763 REVERSE LENGTH=264)

HSP 1 Score: 75.485 bits (184), Expect = 1.804e-15
Identity = 32/70 (45.71%), Postives = 45/70 (64.29%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCLEAC 268
            K +   +S CVG+C + CK P+Q F K+ +G+P+ MEP+F D S +  FG  PP  EDD  +K+PC E C
Sbjct:  185 KCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFEFC 254

HSP 2 Score: 23.8682 bits (50), Expect = 1.804e-15
Identity = 8/21 (38.10%), Postives = 16/21 (76.19%), Query Frame = 3

Query:   24 DGTSCNSGVYVERCKYLEKAS 86
            +G    S VY+E+C++LE+++
Sbjct:  173 NGRKEKSVVYIEKCRFLEQSN 193

[Close]

BLAST of FG537574 vs. TAIR peptide
Match: AT1G64680.1 (| Symbols: | unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). | chr1:24036071-24037062 FORWARD LENGTH=250)

HSP 1 Score: 74.3294 bits (181), Expect = 3.963e-15
Identity = 36/91 (39.56%), Postives = 50/91 (54.95%), Query Frame = 2

Query:   59 KMQVSRESKCVGICINTCKFPTQTFFKDHVGVPLLMEPNFADYSFQFRFGVLPPLPEDDAVVKEPCL-EACPIASQRRMAAKNIGAIACPK 328
            K +    S CVG+C+N CK PTQ FF +  G+PL M PN+ D S +  +G  PP  E+D   K+PCL + C ++        N  +  CPK
Sbjct:  165 KCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPCLADICSMS--------NPSSPICPK 247

HSP 2 Score: 23.8682 bits (50), Expect = 3.963e-15
Identity = 8/20 (40.00%), Postives = 14/20 (70.00%), Query Frame = 3

Query:   24 DGTSCNSGVYVERCKYLEKA 83
            +G    SGV +++C+YLE +
Sbjct:  153 NGVKQRSGVRIKKCRYLENS 172

The following BLAST results are available for this feature:

BLAST of FG537574 vs. TrEMBL
BLAST of FG537574 vs. TAIR peptide

BLAST of FG537574 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TMB0_SOYBN	1.105e-42	78.82	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RNC7_RICCO	2.381e-39	71.43	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9GSU3_POPTR	3.380e-38	81.69	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7U2J7_VITVI	5.116e-36	64.37	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6SY57_SOYBN	3.515e-34	64.71	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7M463_ARALY	1.268e-30	67.95	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q8W4C4_ARATH	1.269e-30	67.95	Putative uncharacterized protein OS=Arabidopsis th...	[more]
A9NQE7_PICSI	9.998e-27	51.11	Putative uncharacterized protein OS=Picea sitchens...	[more]
C5YZG8_SORBI	1.547e-23	57.61	Putative uncharacterized protein Sb09g002570 OS=So...	[more]
C0PPG3_MAIZE	1.547e-23	57.61	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of FG537574 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G01995.1	1.003e-32	67.95	\| Symbols: \| unknown protein; BEST Arabidopsis th...	[more]
AT1G03055.1	1.804e-15	45.71	\| Symbols: \| unknown protein; BEST Arabidopsis th...	[more]
AT1G64680.1	3.963e-15	39.56	\| Symbols: \| unknown protein; BEST Arabidopsis th...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 32..46 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG537574 ID=FG537574; Name=FG537574; organism=Pisum sativum; type=EST; length=563bp
AAGTCAATTCTGTAGACCTACCAGATGGAACCTCCTGCAATAGCGGGGTG
TATGTTGAAAGATGCAAGTATCTAGAGAAAGCAAGTGTGTTGGTATATGC
ATTAACACTTGTAAGTTTCCGACACAGACCTTCTTCAAGGATCATGTGGG
AGTTCCTTTACTAATGGAGCCCAACTTTGCTGATTACAGCTTTCAGTTCA
GATTTGGAGTGCTTCCCCCACTACCTGAAGATGACGCGGTCGTGAAGGAG
CCTTGCTTGGAAGCATGCCCAATTGCTTCCCAACGAAGAATGGCTGCCAA
AAACATAGGTGCCATTGCTTGCCCAAAGACATGAACATATTTTTCTTAGG
CATAGAGCTTTGAGAGTATTGATATACACTTTCCATTACATCCTGCACAT
TTTTAGCCCCAACTTGTGGTTGGAGATGGAGCTGAATGCACGAGCTTTCA
TCATAAAGATAATTCTGTTTTGGCTAAGGTCATATTTGAAATTATCAATG
TAACTCATCTGTGTAATTAGATAACTAAATTAACCTTGGACTTATATATT
TTTATGAATTATG

Search form