FG537581

EST Overview


Name	FG537581
Unique Name	FG537581
Type	EST
Organism	Pisum sativum (pea)
Sequence length	578

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B7FMB1_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.426e-14
Identity = 46/87 (52.87%), Postives = 46/87 (52.87%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPTP 263
            NIDDVDKTMDEINEQTENMKQIQEALS PIG                           Q           VPAGRQP RP PAKPTP
Sbjct:  120 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPTFTAPAPSMQVPAGRQPARPAPAKPTP 206

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B9RKD5_RICCO (Charged multivesicular body protein 4b, putative OS=Ricinus communis GN=RCOM_1048510 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.651e-12
Identity = 43/86 (50.00%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQEALSTPIG                           Q           VPAGRQP RP   K T
Sbjct:  118 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELEGAELEEQLLQPATTAPAAPVQVPAGRQPNRPAAQKQT 203

[Close]

BLAST of FG537581 vs. TrEMBL
Match: A5C1M4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007873 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.476e-11
Identity = 42/86 (48.84%), Postives = 44/86 (51.16%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVD+TMDEINEQTENMKQIQEALSTPIG                           Q          +VPAGRQP R  P K T
Sbjct:  118 NIDDVDQTMDEINEQTENMKQIQEALSTPIGSAADFDEDELEAELEELEGFELEEQLLQPATTAPAAPVHVPAGRQPARAAPQKRT 203

[Close]

BLAST of FG537581 vs. TrEMBL
Match: A5B271_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021984 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.927e-11
Identity = 41/86 (47.67%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDD+DKTMDEINEQTENMKQIQEALSTPIG                           Q           +PA RQP RP P K T
Sbjct:  120 NIDDLDKTMDEINEQTENMKQIQEALSTPIGSAADFDEDELEAELEELEGAELEEQLLQPTTTAPAAPVSIPAARQPARPAPQKST 205

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B6T0C3_MAIZE (Charged multivesicular body protein 4b OS=Zea mays PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.249e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALSTP+G                           +          +VP  +QP RP P K T
Sbjct:  122 NIDDVDKTMDEINEQTENMKQIQDALSTPLGASADFDEDELEAELEELEGAELESQLLEPVAALPVHPVHVPGNKQPARPAPQKAT 207

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B4F8L9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.249e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALSTP+G                           +          +VP  +QP RP P K T
Sbjct:  122 NIDDVDKTMDEINEQTENMKQIQDALSTPLGASADFDEDELEAELEELEGAELESQLLEPVAALPVHPVHVPGNKQPARPAPQKAT 207

[Close]

BLAST of FG537581 vs. TrEMBL
Match: C5XR17_SORBI (Putative uncharacterized protein Sb03g027900 OS=Sorghum bicolor GN=Sb03g027900 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.631e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALS P+G                           +          +VPA +QP RP P K T
Sbjct:  122 NIDDVDKTMDEINEQTENMKQIQDALSAPLGASADFDEDELEAELEELEGAELESQLLEPVAAPPVHPVHVPANKQPARPAPQKAT 207

[Close]

BLAST of FG537581 vs. TrEMBL
Match: C5WW60_SORBI (Putative uncharacterized protein Sb01g004370 OS=Sorghum bicolor GN=Sb01g004370 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.631e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALS P+G                           +          +VPA +QP RP P K T
Sbjct:   91 NIDDVDKTMDEINEQTENMKQIQDALSAPLGAAADFDEDELEAELEELEGAELESQLLEPVAAPPMHPVHVPANKQPARPAPQKAT 176

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B4FNG0_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.131e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALS P+G                           +          +VPA +QP RP P K T
Sbjct:  151 NIDDVDKTMDEINEQTENMKQIQDALSAPLGAFADFDEDELEAELEELEGAELESQLLEPVAAPPVHPVHVPANKQPARPAPQKVT 236

[Close]

BLAST of FG537581 vs. TrEMBL
Match: B4FGC0_MAIZE (Charged multivesicular body protein 4b OS=Zea mays PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.131e-10
Identity = 38/86 (44.19%), Postives = 43/86 (50.00%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSYVPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQ+ALS P+G                           +          +VPA +QP RP P K T
Sbjct:  122 NIDDVDKTMDEINEQTENMKQIQDALSAPLGAFADFDEDELEAELEELEGAELESQLLEPVAAPPVHPVHVPANKQPARPAPQKVT 207

[Close]

BLAST of FG537581 vs. SwissProt
Match: VP322_ARATH (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana GN=VPS32.2 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 8.126e-13
Identity = 44/87 (50.57%), Postives = 46/87 (52.87%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSY-VPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQEAL+TP+G                           Q         S  VPAGRQP RPVP K T
Sbjct:  118 NIDDVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRT 204

[Close]

BLAST of FG537581 vs. SwissProt
Match: VP321_ARATH (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana GN=VPS32.1 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.195e-9
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIG 95
            NIDDVDKTMDEINEQTENMKQIQEALS P G
Sbjct:  119 NIDDVDKTMDEINEQTENMKQIQEALSAPFG 149

[Close]

BLAST of FG537581 vs. TAIR peptide
Match: AT4G29160.3 (| Symbols: SNF7.1 | SNF7 family protein | chr4:14381019-14382342 FORWARD LENGTH=219)

HSP 1 Score: 73.559 bits (179), Expect = 8.614e-14
Identity = 44/87 (50.57%), Postives = 46/87 (52.87%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSY-VPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQEAL+TP+G                           Q         S  VPAGRQP RPVP K T
Sbjct:  118 NIDDVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRT 204

[Close]

BLAST of FG537581 vs. TAIR peptide
Match: AT4G29160.2 (| Symbols: SNF7.1 | SNF7 family protein | chr4:14381350-14382342 FORWARD LENGTH=192)

HSP 1 Score: 73.559 bits (179), Expect = 8.614e-14
Identity = 44/87 (50.57%), Postives = 46/87 (52.87%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSY-VPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQEAL+TP+G                           Q         S  VPAGRQP RPVP K T
Sbjct:   91 NIDDVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRT 177

[Close]

BLAST of FG537581 vs. TAIR peptide
Match: AT4G29160.1 (| Symbols: SNF7.1 | SNF7 family protein | chr4:14381019-14382342 FORWARD LENGTH=219)

HSP 1 Score: 73.559 bits (179), Expect = 8.614e-14
Identity = 44/87 (50.57%), Postives = 46/87 (52.87%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIGXXXXXXXXXXXXXXXXXXXXXXXXXXXQXXXXXXXXXSY-VPAGRQPTRPVPAKPT 260
            NIDDVDKTMDEINEQTENMKQIQEAL+TP+G                           Q         S  VPAGRQP RPVP K T
Sbjct:  118 NIDDVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRT 204

[Close]

BLAST of FG537581 vs. TAIR peptide
Match: AT2G19830.1 (| Symbols: SNF7.2, VPS32 | SNF7 family protein | chr2:8558101-8559389 REVERSE LENGTH=213)

HSP 1 Score: 61.6178 bits (148), Expect = 3.388e-10
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 3

Query:    3 NIDDVDKTMDEINEQTENMKQIQEALSTPIG 95
            NIDDVDKTMDEINEQTENMKQIQEALS P G
Sbjct:  119 NIDDVDKTMDEINEQTENMKQIQEALSAPFG 149

The following BLAST results are available for this feature:

BLAST of FG537581 vs. TrEMBL
BLAST of FG537581 vs. SwissProt
BLAST of FG537581 vs. TAIR peptide

BLAST of FG537581 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FMB1_MEDTR	1.426e-14	52.87	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9RKD5_RICCO	8.651e-12	50.00	Charged multivesicular body protein 4b, putative O...	[more]
A5C1M4_VITVI	1.476e-11	48.84	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A5B271_VITVI	1.927e-11	47.67	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B6T0C3_MAIZE	1.249e-10	44.19	Charged multivesicular body protein 4b OS=Zea mays...	[more]
B4F8L9_MAIZE	1.249e-10	44.19	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5XR17_SORBI	1.631e-10	44.19	Putative uncharacterized protein Sb03g027900 OS=So...	[more]
C5WW60_SORBI	1.631e-10	44.19	Putative uncharacterized protein Sb01g004370 OS=So...	[more]
B4FNG0_MAIZE	2.131e-10	44.19	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FGC0_MAIZE	2.131e-10	44.19	Charged multivesicular body protein 4b OS=Zea mays...	[more]

BLAST of FG537581 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
VP322_ARATH	8.126e-13	50.57	Vacuolar protein sorting-associated protein 32 hom...	[more]
VP321_ARATH	3.195e-9	93.55	Vacuolar protein sorting-associated protein 32 hom...	[more]

BLAST of FG537581 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G29160.3	8.614e-14	50.57	\| Symbols: SNF7.1 \| SNF7 family protein \| chr4:143...	[more]
AT4G29160.2	8.614e-14	50.57	\| Symbols: SNF7.1 \| SNF7 family protein \| chr4:143...	[more]
AT4G29160.1	8.614e-14	50.57	\| Symbols: SNF7.1 \| SNF7 family protein \| chr4:143...	[more]
AT2G19830.1	3.388e-10	93.55	\| Symbols: SNF7.2, VPS32 \| SNF7 family protein \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 7..13 score
None	No IPR available	SEG	seg	seg	coord: 32..58 score: NAcoord: 60..68 score
None	No IPR available	SEG	seg	seg	coord: 28..40 score
IPR005024	Snf7	PFAM	PF03357	Snf7	coord: 1..69 score: 4.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG537581 ID=FG537581; Name=FG537581; organism=Pisum sativum; type=EST; length=578bp
TGAATATTGACGATGTTGACAAAACCATGGATGAGATAAATGAACAGACT
GAGAACATGAAGCAGATTCAAGAAGCATTGTCAACTCCAATTGGTGCAGC
AGCTGATTTTGATGAGGATGAACTAGAAGCAGAACTTGAAGAATTGGAGA
GTGCTGAGTTAGAAGAACAGCTTCTTCAGCCAGCAACTACAGCTCCTGCT
GCCCCATCGTACGTCCCAGCTGGGCGGCAACCTACACGCCCTGTTCCTGC
AAAGCCAACTCCCGAGGAAGATGAATTGGCAGCTTTGCAGGCTGAGATGG
CACTTTGATCGGGTACCTTTTCTCGTCTGTGTCACTACCGATGGATTTGT
GTTGTTATCTATGCATTAGTTTCTTTTATCTGAATTCTGAACTGGTGTTG
CATTTAGTATGCAAGTTAAATGATATGGTTATGTCACATTTTCATGTTTT
GCCTATGCCAGAAATATTTTAGGTAGTTGTTCTATTTGGTTTTGCCACGG
CTGGCTTAGTTTAAGCCAGAATTTCGCTGGCTTTGTTAACCATGACGATA
AGTGTCTTCCACGATATATTTGTAATAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR005024	Snf7_fam

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