AM161974

EST Overview


Name	AM161974
Unique Name	AM161974
Type	EST
Organism	Pisum sativum (pea)
Sequence length	329

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM161974 vs. TrEMBL
Match: C6TGA9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.775e-53
Identity = 102/109 (93.58%), Postives = 107/109 (98.17%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PEDDYMLPLSEAEVIR GSDITLVGWGAQLSIMEQAC+DAEKEGISCELIDLKTLIPWDKETVEASV KTGRLL+SHEAP+TGGFGAEISASI+ERCFSRLEAPVAR+C
Sbjct:  217 PEDDYMLPLSEAEVIRQGSDITLVGWGAQLSIMEQACLDAEKEGISCELIDLKTLIPWDKETVEASVNKTGRLLVSHEAPITGGFGAEISASIVERCFSRLEAPVARIC 325

[Close]

BLAST of AM161974 vs. TrEMBL
Match: B5LAT4_CAPAN (Putative branched-chain alpha-keto acid dehydrogenase E1 beta subunit OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 4.371e-52
Identity = 100/109 (91.74%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PEDDYMLPLSEAEV+R G+DITLVGWGAQLSIMEQAC++A KEGISCELIDLKTLIPWDKETVEASVKKTGRLL+SHEAPVTGGFGAEISASI ERCF+RLEAPVARVC
Sbjct:  222 PEDDYMLPLSEAEVLREGTDITLVGWGAQLSIMEQACVEAAKEGISCELIDLKTLIPWDKETVEASVKKTGRLLVSHEAPVTGGFGAEISASIAERCFTRLEAPVARVC 330

[Close]

BLAST of AM161974 vs. TrEMBL
Match: B9GXX6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1074695 PE=4 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 7.456e-52
Identity = 101/109 (92.66%), Postives = 105/109 (96.33%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYMLPLSEAEVIR GSDITLVGWGAQLSIMEQAC DAEKEGISCELIDLKTLIPWDKETVEASV+KTG+LLISHEAPVTGGFGAEISASI+ERCF RLEAPVAR+C
Sbjct:  229 PEHDYMLPLSEAEVIREGSDITLVGWGAQLSIMEQACFDAEKEGISCELIDLKTLIPWDKETVEASVRKTGKLLISHEAPVTGGFGAEISASIVERCFLRLEAPVARIC 337

[Close]

BLAST of AM161974 vs. TrEMBL
Match: B9SBN1_RICCO (2-oxoisovalerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_0342870 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 3.701e-51
Identity = 99/109 (90.83%), Postives = 105/109 (96.33%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYMLPLSEAEVI+ GSDITLVGWGAQLS+MEQAC DAEK+GISCELIDLKTLIPWDKETVEASV+KTGRLLISHEAP+TGGFGAEISASI+ERCF RLEAPVARVC
Sbjct:  226 PEHDYMLPLSEAEVIQEGSDITLVGWGAQLSVMEQACTDAEKDGISCELIDLKTLIPWDKETVEASVRKTGRLLISHEAPITGGFGAEISASIVERCFLRLEAPVARVC 334

[Close]

BLAST of AM161974 vs. TrEMBL
Match: D7KMA1_ARALY (BCDH BETA1 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_892547 PE=4 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 4.833e-51
Identity = 98/109 (89.91%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYM+PLSEAEVIR G+DITLVGWGAQL+IMEQAC+DAEKEGISCELIDLKTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+ILERCF +LEAPV+RVC
Sbjct:  213 PEHDYMIPLSEAEVIREGNDITLVGWGAQLTIMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISATILERCFLKLEAPVSRVC 321

[Close]

BLAST of AM161974 vs. TrEMBL
Match: Q9SAV3_ARATH (At1g55510 OS=Arabidopsis thaliana GN=At1g55510 PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.312e-51
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYM+PLSEAEVIR G+DITLVGWGAQL++MEQAC+DAEKEGISCELIDLKTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+ILERCF +LEAPV+RVC
Sbjct:  213 PEHDYMIPLSEAEVIREGNDITLVGWGAQLTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISATILERCFLKLEAPVSRVC 321

[Close]

BLAST of AM161974 vs. TrEMBL
Match: O82450_ARATH (Branched-chain alpha-keto acid decarboxylase E1 beta subunit OS=Arabidopsis thaliana GN=BCDH beta1 PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.312e-51
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYM+PLSEAEVIR G+DITLVGWGAQL++MEQAC+DAEKEGISCELIDLKTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+ILERCF +LEAPV+RVC
Sbjct:  213 PEHDYMIPLSEAEVIREGNDITLVGWGAQLTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISATILERCFLKLEAPVSRVC 321

[Close]

BLAST of AM161974 vs. TrEMBL
Match: D7SLN4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025496001 PE=4 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.312e-51
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYMLPLSEAEVIR G+DITLVGWGAQL++MEQACIDAEKEGISCELIDL+TL+PWDKETVEASV+KTGRLL+SHEAPVTGGFGAEISAS++ERCF RLEAPVARVC
Sbjct:  219 PEHDYMLPLSEAEVIRQGTDITLVGWGAQLAVMEQACIDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLVSHEAPVTGGFGAEISASMVERCFLRLEAPVARVC 327

[Close]

BLAST of AM161974 vs. TrEMBL
Match: C6TKP7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.312e-51
Identity = 97/107 (90.65%), Postives = 105/107 (98.13%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVAR 322
            PEDDYMLPLSEAEVIR GSD+TLVGWGAQL+IMEQAC+DAEKEGISCELIDLKTLIPWDKETVE+SV KTGRLL+SHEAP+TGGFGAEISASI+ERCFSRLEAPVA+
Sbjct:   66 PEDDYMLPLSEAEVIRQGSDVTLVGWGAQLAIMEQACLDAEKEGISCELIDLKTLIPWDKETVESSVNKTGRLLVSHEAPITGGFGAEISASIVERCFSRLEAPVAK 172

[Close]

BLAST of AM161974 vs. TrEMBL
Match: A5B765_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_037525 PE=4 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.312e-51
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYMLPLSEAEVIR G+DITLVGWGAQL++MEQACIDAEKEGISCELIDL+TL+PWDKETVEASV+KTGRLL+SHEAPVTGGFGAEISAS++ERCF RLEAPVARVC
Sbjct:  182 PEHDYMLPLSEAEVIRQGTDITLVGWGAQLAVMEQACIDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLVSHEAPVTGGFGAEISASMVERCFLRLEAPVARVC 290

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_DICDI (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Dictyostelium discoideum GN=bkdB PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 8.382e-34
Identity = 68/110 (61.82%), Postives = 87/110 (79.09%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKE-GISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            P  DY +PL +A +++ G DIT++GWGAQ+ ++ QA   AE++ GISCELIDL+T+ PWD ETV  SVKKTGR++ISHEAP TGG+ AEISA+I ERCF  LEAP+ RVC
Sbjct:  230 PIGDYEIPLGKARIVKEGKDITIIGWGAQMRVLLQAVNMAEEKLGISCELIDLRTIQPWDVETVVESVKKTGRVVISHEAPKTGGWAAEISATIQERCFLHLEAPIQRVC 339

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_RAT (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus GN=Bckdhb PE=1 SV=3)

HSP 1 Score: 140.969 bits (354), Expect = 1.867e-33
Identity = 66/110 (60.00%), Postives = 88/110 (80.00%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIM-EQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            P + Y +PLS+AEVI+ GSD+TLV WG Q+ ++ E A +  EK G+SCE+IDL+T++PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CF  LEAP++RVC
Sbjct:  250 PVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVC 359

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_MOUSE (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus GN=Bckdhb PE=2 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 1.867e-33
Identity = 66/110 (60.00%), Postives = 88/110 (80.00%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIM-EQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            P + Y +PLS+AEVI+ GSD+TLV WG Q+ ++ E A +  EK G+SCE+IDL+T++PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CF  LEAP++RVC
Sbjct:  250 PVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVC 359

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_HUMAN (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Homo sapiens GN=BCKDHB PE=1 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 3.185e-33
Identity = 66/110 (60.00%), Postives = 88/110 (80.00%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIM-EQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            P + Y +PLS+AEVI+ GSD+TLV WG Q+ ++ E A +  EK G+SCE+IDL+T+IPWD +T+  SV KTGRLLISHEAP+TGGF +EIS+++ E CF  LEAP++RVC
Sbjct:  252 PIEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAKEKLGVSCEVIDLRTIIPWDVDTICKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVC 361

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_BOVIN (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus GN=BCKDHB PE=1 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 3.185e-33
Identity = 65/110 (59.09%), Postives = 88/110 (80.00%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIM-EQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            P + Y +PLS+AEVI+ GSD+TLV WG Q+ ++ E A +  EK G+SCE+IDL+T++PWD +TV  SV KTGRLL+SHEAP+TGGF +EIS+++ E CF  LEAP++RVC
Sbjct:  252 PVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVAAMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLVSHEAPLTGGFASEISSTVQEECFLNLEAPISRVC 361

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_PSEAE (2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas aeruginosa GN=bkdA2 PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.256e-21
Identity = 49/108 (45.37%), Postives = 75/108 (69.44%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            P+  Y +PL +A ++RPG+ +T++ +G  + + + A   A++ G+  E+IDL++L P D ET+ ASVKKTGR +I+HEA  T GFGAE+ + + E CF  LEAP+ RV
Sbjct:  214 PDGYYKVPLDKAAIVRPGAALTVLTYGTMVYVAQAA---ADETGLDAEIIDLRSLWPLDLETIVASVKKTGRCVIAHEATRTCGFGAELMSLVQEHCFHHLEAPIERV 318

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_PSEPU (2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.813e-20
Identity = 47/108 (43.52%), Postives = 73/108 (67.59%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            P+  Y +PL +A + RPG+D++++ +G  + + + A   AE+ G+  E+IDL++L P D +T+  SVKKTGR ++ HEA  T GFGAE+ + + E CF  LEAP+ RV
Sbjct:  203 PDGYYTVPLDKAAITRPGNDVSVLTYGTTVYVAQVA---AEESGVDAEVIDLRSLWPLDLDTIVESVKKTGRCVVVHEATRTCGFGAELVSLVQEHCFHHLEAPIERV 307

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODPB_BACST (Pyruvate dehydrogenase E1 component subunit beta OS=Bacillus stearothermophilus GN=pdhB PE=1 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 4.039e-20
Identity = 48/108 (44.44%), Postives = 69/108 (63.89%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            PE +Y +P+ +A++ R G DIT++ +GA +    +A  + EKEGIS E++DL+T+ P D ET+  SV+KTGR ++  EA    G  A + A I ER    LEAPV RV
Sbjct:  185 PEGEYTIPIGKADIKREGKDITIIAYGAMVHESLKAAAELEKEGISAEVVDLRTVQPLDIETIIGSVEKTGRAIVVQEAQRQAGIAANVVAEINERAILSLEAPVLRV 292

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODBB_THET8 (2-oxoisovalerate dehydrogenase subunit beta OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=TTHA0230 PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 5.275e-20
Identity = 47/108 (43.52%), Postives = 70/108 (64.81%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            PE+DY LP+ +A + R G D+TL+G+G  +  + QA  +  K G+S E++DL+TL+PWD E V  SV KTGR+++  +AP    F +E++A+I E     L AP  RV
Sbjct:  185 PEEDYTLPIGKAALRREGKDLTLIGYGTVMPEVLQAAAELAKAGVSAEVLDLRTLMPWDYEAVMNSVAKTGRVVLVSDAPRHASFVSEVAATIAEDLLDMLLAPPIRV 292

[Close]

BLAST of AM161974 vs. SwissProt
Match: ODPB_STAES (Pyruvate dehydrogenase E1 component subunit beta OS=Staphylococcus epidermidis (strain ATCC 12228) GN=pdhB PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.998e-20
Identity = 47/108 (43.52%), Postives = 73/108 (67.59%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            PE++Y + + +A V + G+DITL+ +GA +    +A  + EK+G S E+IDL+T+ P D +T+ ASV+KTGR ++  EA    G GA+++A + ER    LEAP+ARV
Sbjct:  185 PEEEYKIDIGKANVKKEGNDITLISYGAMVQESLKAAEELEKDGYSVEVIDLRTVQPIDIDTLVASVEKTGRAVVVQEAQRQAGVGAQVAAELAERAILSLEAPIARV 292

[Close]

BLAST of AM161974 vs. TAIR peptide
Match: AT1G55510.1 (| Symbols: BCDH BETA1 | branched-chain alpha-keto acid decarboxylase E1 beta subunit | chr1:20723482-20725505 FORWARD LENGTH=352)

HSP 1 Score: 203.371 bits (516), Expect = 2.428e-53
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PE DYM+PLSEAEVIR G+DITLVGWGAQL++MEQAC+DAEKEGISCELIDLKTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+ILERCF +LEAPV+RVC
Sbjct:  213 PEHDYMIPLSEAEVIREGNDITLVGWGAQLTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISATILERCFLKLEAPVSRVC 321

[Close]

BLAST of AM161974 vs. TAIR peptide
Match: AT3G13450.1 (| Symbols: DIN4 | Transketolase family protein | chr3:4382340-4384295 REVERSE LENGTH=358)

HSP 1 Score: 199.134 bits (505), Expect = 4.580e-52
Identity = 95/109 (87.16%), Postives = 104/109 (95.41%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARVC 328
            PEDDYM+PLSEAEV+R GSDITLVGWGAQL+IMEQAC+DAE EGISCELIDLKTLIPWDKE VE SV+KTGRLLISHEAPVTGGFGAEI+A+I+ERCF RLEAPV+RVC
Sbjct:  219 PEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVC 327

[Close]

BLAST of AM161974 vs. TAIR peptide
Match: AT5G50850.1 (| Symbols: MAB1 | Transketolase family protein | chr5:20689671-20692976 FORWARD LENGTH=363)

HSP 1 Score: 80.4925 bits (197), Expect = 2.374e-16
Identity = 41/107 (38.32%), Postives = 66/107 (61.68%), Query Frame = 2

Query:    5 EDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPVARV 325
            +  + LP+ +A++ R G D+T+V +   +    +A     +EGIS E+I+L+++ P D+ T+ ASV+KT RL+   E     G  AEI AS++E  FS L+APV R+
Sbjct:  221 DSSFCLPIGKAKIEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVCAEICASVVEESFSYLDAPVERI 327

[Close]

BLAST of AM161974 vs. TAIR peptide
Match: AT1G30120.1 (| Symbols: PDH-E1 BETA | pyruvate dehydrogenase E1 beta | chr1:10584350-10586477 REVERSE LENGTH=406)

HSP 1 Score: 80.4925 bits (197), Expect = 2.374e-16
Identity = 43/105 (40.95%), Postives = 64/105 (60.95%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPV 316
            P++DY+  L EAE++RPG  IT++ +      + QA      +G   E+ID+++L P+D  T+  SVKKT R+LI  E   TGG GA ++A+I E     L+APV
Sbjct:  266 PDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAPV 370

[Close]

BLAST of AM161974 vs. TAIR peptide
Match: AT2G34590.1 (| Symbols: | Transketolase family protein | chr2:14568956-14570844 REVERSE LENGTH=406)

HSP 1 Score: 78.1814 bits (191), Expect = 1.178e-15
Identity = 42/105 (40.00%), Postives = 64/105 (60.95%), Query Frame = 2

Query:    2 PEDDYMLPLSEAEVIRPGSDITLVGWGAQLSIMEQACIDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASILERCFSRLEAPV 316
            P+++Y+  L EAE++RPG  IT++ +      + QA      +G   E+ID+++L P+D  T+  SVKKT R+LI  E   TGG GA ++A+I E     L+APV
Sbjct:  266 PDEEYICNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAPV 370

The following BLAST results are available for this feature:

BLAST of AM161974 vs. TrEMBL
BLAST of AM161974 vs. SwissProt
BLAST of AM161974 vs. TAIR peptide

BLAST of AM161974 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TGA9_SOYBN	1.775e-53	93.58	Putative uncharacterized protein OS=Glycine max PE...	[more]
B5LAT4_CAPAN	4.371e-52	91.74	Putative branched-chain alpha-keto acid dehydrogen...	[more]
B9GXX6_POPTR	7.456e-52	92.66	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SBN1_RICCO	3.701e-51	90.83	2-oxoisovalerate dehydrogenase, putative OS=Ricinu...	[more]
D7KMA1_ARALY	4.833e-51	89.91	BCDH BETA1 OS=Arabidopsis lyrata subsp. lyrata GN=...	[more]
Q9SAV3_ARATH	6.312e-51	88.99	At1g55510 OS=Arabidopsis thaliana GN=At1g55510 PE=...	[more]
O82450_ARATH	6.312e-51	88.99	Branched-chain alpha-keto acid decarboxylase E1 be...	[more]
D7SLN4_VITVI	6.312e-51	88.99	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6TKP7_SOYBN	6.312e-51	90.65	Putative uncharacterized protein OS=Glycine max PE...	[more]
A5B765_VITVI	6.312e-51	88.99	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of AM161974 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ODBB_DICDI	8.382e-34	61.82	2-oxoisovalerate dehydrogenase subunit beta, mitoc...	[more]
ODBB_RAT	1.867e-33	60.00	2-oxoisovalerate dehydrogenase subunit beta, mitoc...	[more]
ODBB_MOUSE	1.867e-33	60.00	2-oxoisovalerate dehydrogenase subunit beta, mitoc...	[more]
ODBB_HUMAN	3.185e-33	60.00	2-oxoisovalerate dehydrogenase subunit beta, mitoc...	[more]
ODBB_BOVIN	3.185e-33	59.09	2-oxoisovalerate dehydrogenase subunit beta, mitoc...	[more]
ODBB_PSEAE	1.256e-21	45.37	2-oxoisovalerate dehydrogenase subunit beta OS=Pse...	[more]
ODBB_PSEPU	1.813e-20	43.52	2-oxoisovalerate dehydrogenase subunit beta OS=Pse...	[more]
ODPB_BACST	4.039e-20	44.44	Pyruvate dehydrogenase E1 component subunit beta O...	[more]
ODBB_THET8	5.275e-20	43.52	2-oxoisovalerate dehydrogenase subunit beta OS=The...	[more]
ODPB_STAES	8.998e-20	43.52	Pyruvate dehydrogenase E1 component subunit beta O...	[more]

BLAST of AM161974 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G55510.1	2.428e-53	88.99	\| Symbols: BCDH BETA1 \| branched-chain alpha-keto ...	[more]
AT3G13450.1	4.580e-52	87.16	\| Symbols: DIN4 \| Transketolase family protein \| c...	[more]
AT5G50850.1	2.374e-16	38.32	\| Symbols: MAB1 \| Transketolase family protein \| c...	[more]
AT1G30120.1	2.374e-16	40.95	\| Symbols: PDH-E1 BETA \| pyruvate dehydrogenase E1...	[more]
AT2G34590.1	1.178e-15	40.00	\| Symbols: \| Transketolase family protein \| chr2:...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005476	Transketolase, C-terminal	PFAM	PF02780	Transketolase_C	coord: 11..109 score: 5.9
IPR009014	Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II	SUPERFAMILY	52922	TK C-terminal domain-like	coord: 4..109 score: 2.2
IPR015941	Transketolase-like, C-terminal	GENE3D	3.40.50.920	no description	coord: 13..109 score: 6.2
None	No IPR available	PANTHER	PTHR11624	DEHYDROGENASE RELATED	coord: 1..109 score: 1
None	No IPR available	PANTHER	PTHR11624:SF21	BRANCHED CHAIN ALPHA KETO ACID DEHYDROGENASE E1 BETA SUBUNIT	coord: 1..109 score: 1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161974 ID=AM161974; Name=AM161974; organism=Pisum sativum; type=EST; length=329bp
ACCGGAAGATGATTACATGTTGCCATTATCTGAAGCCGAGGTGATTCGGC
CAGGAAGCGATATTACACTTGTTGGTTGGGGAGCTCAATTATCTATCATG
GAACAAGCTTGTATTGATGCAGAAAAGGAGGGAATTTCTTGTGAATTGAT
AGATCTGAAGACACTAATTCCTTGGGATAAAGAAACAGTGGAGGCATCAG
TAAAGAAGACAGGAAGACTTCTTATTAGTCATGAAGCTCCTGTGACTGGT
GGTTTTGGCGCTGAAATCTCGGCTTCAATTCTTGAACGCTGCTTCTCAAG
GTTAGAGGCTCCTGTAGCAAGAGTTTGTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR005476	Transketolase, C-terminal
IPR009014	Transketo_C/PFOR_II
IPR015941	Transketolase-like, C-terminal

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