CD860371

EST Overview


Name	CD860371
Unique Name	CD860371
Type	EST
Organism	Pisum sativum (pea)
Sequence length	255

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03

Homology

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q4ZGM9_MEDTR (13-hydroperoxide lyase OS=Medicago truncatula GN=HPL3 PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 2.550e-36
Identity = 69/84 (82.14%), Postives = 81/84 (96.43%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            +PPTFPFFT+VNPN+IA+LDCK+FSHL DM+LVDKKDVLVGDFVPSVEFTGNIRVGVY+DVS+PQH+K+K FSM +LK+SSSIW
Sbjct:   72 VPPTFPFFTNVNPNIIAVLDCKSFSHLFDMDLVDKKDVLVGDFVPSVEFTGNIRVGVYQDVSEPQHAKAKSFSMNILKQSSSIW 155

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q9STA2_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v2 PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 4.349e-36
Identity = 69/84 (82.14%), Postives = 81/84 (96.43%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            IPPTFPFFT+VNPN+IA+LDCK+FSHL DM+LVDK+DVLVGDFVPSVEFTGNIRVGVY+DVS+PQH+K+K FSM +LK+SSSIW
Sbjct:   72 IPPTFPFFTNVNPNIIAVLDCKSFSHLFDMDLVDKRDVLVGDFVPSVEFTGNIRVGVYQDVSEPQHAKAKNFSMNILKQSSSIW 155

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q9STA3_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v1 PE=2 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 5.681e-36
Identity = 68/84 (80.95%), Postives = 81/84 (96.43%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            +PPTFPFFT+VNPN+IA+LDCK+FSHL DM+LVDK+DVLVGDFVPSVEFTGNIRVGVY+DVS+PQH+K+K FSM +LK+SSSIW
Sbjct:   72 VPPTFPFFTNVNPNIIAVLDCKSFSHLFDMDLVDKRDVLVGDFVPSVEFTGNIRVGVYQDVSEPQHAKAKNFSMNILKQSSSIW 155

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q9STA1_MEDSA (Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v3 PE=2 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 5.681e-36
Identity = 68/84 (80.95%), Postives = 81/84 (96.43%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            +PPTFPFFT+VNPN+IA+LDCK+FSHL DM+LVDK+DVLVGDFVPSVEFTGNIRVGVY+DVS+PQH+K+K FSM +LK+SSSIW
Sbjct:   72 VPPTFPFFTNVNPNIIAVLDCKSFSHLFDMDLVDKRDVLVGDFVPSVEFTGNIRVGVYQDVSEPQHAKAKNFSMNILKQSSSIW 155

[Close]

BLAST of CD860371 vs. TrEMBL
Match: D7MAH9_ARALY (Hydroperoxide lyase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_355235 PE=4 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 4.360e-28
Identity = 54/84 (64.29%), Postives = 71/84 (84.52%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            IPPTFPFF  VNPN++A+LD K+FSHL DM+LVDK+DVL+GDF PS+EF G +RVGVY D ++P+H+K K F+M++LK SS +W
Sbjct:   75 IPPTFPFFGDVNPNIVAVLDVKSFSHLFDMDLVDKRDVLIGDFRPSLEFYGGVRVGVYLDTTEPKHAKIKSFAMEILKRSSKVW 158

[Close]

BLAST of CD860371 vs. TrEMBL
Match: B9STL5_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1016730 PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.268e-27
Identity = 53/84 (63.10%), Postives = 69/84 (82.14%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            +PPTFP FT VNPNVIA+LDCK+F+HL DM++V+KK++LVGDFVP ++FTG+IRV VY D S+P H K K F+M +LK  S +W
Sbjct:   90 VPPTFPLFTGVNPNVIAVLDCKSFAHLFDMDIVEKKNILVGDFVPDIKFTGSIRVSVYLDPSEPLHGKVKNFAMDILKRGSKVW 173

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q9AUD8_PSIGU (Fatty acid hydroperoxide lyase OS=Psidium guajava GN=HPL PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.295e-27
Identity = 51/84 (60.71%), Postives = 71/84 (84.52%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            +PP FPFF++VNPNV+ +LDC++F+HL DME+V+K +VLVGDF+PSV++TGNIRV  Y D S+PQH++ K F+M +LK SS +W
Sbjct:   81 VPPCFPFFSNVNPNVVVVLDCESFAHLFDMEIVEKSNVLVGDFMPSVKYTGNIRVCAYLDTSEPQHAQVKNFAMDILKRSSKVW 164

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q84V86_CITSI (Fatty acid hydroperoxide lyase OS=Citrus sinensis GN=HPL PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.392e-26
Identity = 52/84 (61.90%), Postives = 70/84 (83.33%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            IPPT+P F +VNPNVIA+LDCK+F+HL DME+V+KK++LVGDF+PSV+FTG +RV  Y D S+P+H++ K F+M  LK SS +W
Sbjct:   89 IPPTWPLFLNVNPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKGFAMDTLKRSSKLW 172

[Close]

BLAST of CD860371 vs. TrEMBL
Match: Q76LM3_CITJA (Hydroperoxide lyase OS=Citrus jambhiri GN=RlemHPL PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.392e-26
Identity = 52/84 (61.90%), Postives = 70/84 (83.33%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            IPPT+P F +VNPNVIA+LDCK+F+HL DME+V+KK++LVGDF+PSV+FTG +RV  Y D S+P+H++ K F+M  LK SS +W
Sbjct:   89 IPPTWPLFLNVNPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKGFAMDTLKRSSKLW 172

[Close]

BLAST of CD860371 vs. TrEMBL
Match: D6Q1N0_9ROSI (Hydroperoxide lyase OS=Fortunella margarita GN=HPL PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.392e-26
Identity = 52/84 (61.90%), Postives = 70/84 (83.33%), Query Frame = 3

Query:    3 IPPTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            IPPT+P F +VNPNVIA+LDCK+F+HL DME+V+KK++LVGDF+PSV+FTG +RV  Y D S+P+H++ K F+M  LK SS +W
Sbjct:   89 IPPTWPLFLNVNPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKGFAMDTLKRSSKLW 172

[Close]

BLAST of CD860371 vs. SwissProt
Match: CP74_LINUS (Allene oxide synthase, chloroplastic OS=Linum usitatissimum GN=CYP74A PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.961e-12
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 3

Query:    9 PTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLK 236
            P  PF  S NP VI LLD K+F  L DM  V+KKD+  G ++PS E TG  R+  Y D S+P H+K K+    ++K
Sbjct:  120 PPGPFIAS-NPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIK 194

[Close]

BLAST of CD860371 vs. SwissProt
Match: C74A2_PARAR (Allene oxide synthase OS=Parthenium argentatum GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 8.825e-12
Identity = 34/75 (45.33%), Postives = 47/75 (62.67%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSS 248
            F S NP VI LLD K+F  L D+  V+KKD+  G ++PS + TG  RV  Y D S+P+H++ K     +LK SS+
Sbjct:   61 FVSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLLFFMLKNSSN 135

[Close]

BLAST of CD860371 vs. SwissProt
Match: CP74A_ARATH (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana GN=CYP74A PE=1 SV=3)

HSP 1 Score: 68.5514 bits (166), Expect = 1.153e-11
Identity = 33/73 (45.21%), Postives = 46/73 (63.01%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKES 242
            F + NP V+ALLD K+F  L D++ V+KKD+  G ++PS E TG  R+  Y D S+P+H K K     +LK S
Sbjct:  106 FIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSS 178

[Close]

BLAST of CD860371 vs. SwissProt
Match: C74A2_ORYSJ (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 7.471e-11
Identity = 31/70 (44.29%), Postives = 44/70 (62.86%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVL 233
            F + +P V+ALLD K+F  L D+  V+K+DV  G F+PS   TG  RV  Y D S+P H+K K+  + +L
Sbjct:   61 FMARDPRVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLLLSLL 130

[Close]

BLAST of CD860371 vs. SwissProt
Match: C74A1_ORYSJ (Allene oxide synthase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=CYP74A1 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.139e-9
Identity = 30/70 (42.86%), Postives = 40/70 (57.14%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVL 233
            F + +P V+ALLD  +F  L D  LVDK D+  G F+PS + TG  RV  Y D S+P H+  K     +L
Sbjct:  100 FVARDPRVVALLDAASFPVLFDTSLVDKTDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHAPLKTLLFYLL 169

[Close]

BLAST of CD860371 vs. SwissProt
Match: C74A4_ORYSJ (Allene oxide synthase 4 OS=Oryza sativa subsp. japonica GN=CYP74A4 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.354e-9
Identity = 31/74 (41.89%), Postives = 44/74 (59.46%), Query Frame = 3

Query:    9 PTFPFFTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQV 230
            P  PF    +P V+ALLD ++F  LLD  +VDK D L G F+PS+   G  R   + D +DP+H+K K+  M +
Sbjct:   78 PPGPFLAG-DPRVVALLDARSFRVLLDDSMVDKADTLDGTFMPSLALFGGHRPLAFLDAADPRHAKIKRVVMSL 150

[Close]

BLAST of CD860371 vs. SwissProt
Match: C74A3_ORYSJ (Allene oxide synthase 3 OS=Oryza sativa subsp. japonica GN=CYP74A3 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.354e-9
Identity = 30/69 (43.48%), Postives = 42/69 (60.87%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQV 230
            F S NP V+ALLD ++F  LLD  +VDK D L G ++PS    G  R   + D +DP+H+K K+  M +
Sbjct:   82 FISGNPRVVALLDARSFRVLLDDSMVDKADTLDGTYMPSRALFGGHRPLAFLDAADPRHAKIKRVVMSL 150

[Close]

BLAST of CD860371 vs. TAIR peptide
Match: AT4G15440.1 (| Symbols: HPL1, CYP74B2 | hydroperoxide lyase 1 | chr4:8835869-8838462 FORWARD LENGTH=384)

HSP 1 Score: 75.485 bits (184), Expect = 7.640e-15
Identity = 32/55 (58.18%), Postives = 44/55 (80.00%), Query Frame = 3

Query:   90 MELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKESSSIW 254
            M+LVDK+DVL+GDF PS+ F G +RVGVY D ++P+H+K K F+M+ LK SS +W
Sbjct:    1 MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVW 55

[Close]

BLAST of CD860371 vs. TAIR peptide
Match: AT5G42650.1 (| Symbols: AOS, CYP74A, DDE2 | allene oxide synthase | chr5:17097803-17099359 REVERSE LENGTH=518)

HSP 1 Score: 68.5514 bits (166), Expect = 9.339e-13
Identity = 33/73 (45.21%), Postives = 46/73 (63.01%), Query Frame = 3

Query:   24 FTSVNPNVIALLDCKAFSHLLDMELVDKKDVLVGDFVPSVEFTGNIRVGVYRDVSDPQHSKSKKFSMQVLKES 242
            F + NP V+ALLD K+F  L D++ V+KKD+  G ++PS E TG  R+  Y D S+P+H K K     +LK S
Sbjct:  106 FIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSS 178

The following BLAST results are available for this feature:

BLAST of CD860371 vs. TrEMBL
BLAST of CD860371 vs. SwissProt
BLAST of CD860371 vs. TAIR peptide

BLAST of CD860371 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q4ZGM9_MEDTR	2.550e-36	82.14	13-hydroperoxide lyase OS=Medicago truncatula GN=H...	[more]
Q9STA2_MEDSA	4.349e-36	82.14	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
Q9STA3_MEDSA	5.681e-36	80.95	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
Q9STA1_MEDSA	5.681e-36	80.95	Hydroperoxide lyase OS=Medicago sativa GN=cyp74B4v...	[more]
D7MAH9_ARALY	4.360e-28	64.29	Hydroperoxide lyase OS=Arabidopsis lyrata subsp. l...	[more]
B9STL5_RICCO	1.268e-27	63.10	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
Q9AUD8_PSIGU	6.295e-27	60.71	Fatty acid hydroperoxide lyase OS=Psidium guajava ...	[more]
Q84V86_CITSI	2.392e-26	61.90	Fatty acid hydroperoxide lyase OS=Citrus sinensis ...	[more]
Q76LM3_CITJA	2.392e-26	61.90	Hydroperoxide lyase OS=Citrus jambhiri GN=RlemHPL ...	[more]
D6Q1N0_9ROSI	2.392e-26	61.90	Hydroperoxide lyase OS=Fortunella margarita GN=HPL...	[more]

BLAST of CD860371 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
CP74_LINUS	3.961e-12	46.05	Allene oxide synthase, chloroplastic OS=Linum usit...	[more]
C74A2_PARAR	8.825e-12	45.33	Allene oxide synthase OS=Parthenium argentatum GN=...	[more]
CP74A_ARATH	1.153e-11	45.21	Allene oxide synthase, chloroplastic OS=Arabidopsi...	[more]
C74A2_ORYSJ	7.471e-11	44.29	Allene oxide synthase 2 OS=Oryza sativa subsp. jap...	[more]
C74A1_ORYSJ	3.139e-9	42.86	Allene oxide synthase 1, chloroplastic OS=Oryza sa...	[more]
C74A4_ORYSJ	5.354e-9	41.89	Allene oxide synthase 4 OS=Oryza sativa subsp. jap...	[more]
C74A3_ORYSJ	5.354e-9	43.48	Allene oxide synthase 3 OS=Oryza sativa subsp. jap...	[more]

BLAST of CD860371 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT4G15440.1	7.640e-15	58.18	\| Symbols: HPL1, CYP74B2 \| hydroperoxide lyase 1 \|...	[more]
AT5G42650.1	9.339e-13	45.21	\| Symbols: AOS, CYP74A, DDE2 \| allene oxide syntha...	[more]

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860371 ID=CD860371; Name=CD860371; organism=Pisum sativum; type=EST; length=255bp
ATATTCCTCCAACGTTTCCGTTTTTCACCAGCGTAAATCCCAACGTTATT
GCACTCCTCGATTGCAAGGCTTTCTCTCACCTCTTGGACATGGAGTTAGT
TGACAAAAAAGATGTTCTGGTTGGAGATTTTGTCCCTAGCGTTGAATTCA
CTGGGAATATTAGGGTTGGTGTTTACAGAGATGTTTCTGATCCTCAACAT
TCTAAGTCAAAGAAGTTCTCCATGCAAGTATTGAAAGAAAGCTCAAGCAT
ATGGG

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