CD860421

EST Overview


Name	CD860421
Unique Name	CD860421
Type	EST
Organism	Pisum sativum (pea)
Sequence length	520

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of CD860421 vs. TrEMBL
Match: B7ZEP5_PEA (Small heat-shock protein OS=Pisum sativum GN=hsp16 PE=3 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 1.492e-61
Identity = 123/145 (84.83%), Postives = 123/145 (84.83%), Query Frame = 3

Query:   51 RTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            RTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI                      IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD
Sbjct:   59 RTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIKGEEGAKESEEKEKSGRRFSSRIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 203

HSP 2 Score: 26.9498 bits (58), Expect = 1.492e-61
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 1

Query:   13 MTVTLVFIVTPSL 51
            MTVTL+FIVTPSL
Sbjct:   46 MTVTLMFIVTPSL 58

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BLAST of CD860421 vs. TrEMBL
Match: B9GVE8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_817274 PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 5.419e-40
Identity = 91/146 (62.33%), Postives = 104/146 (71.23%), Query Frame = 3

Query:   60 RDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS----RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            RDD L  +VFDPFSP RSLSQVLNM+D   +NP++SA      RRGWDARET+DAL LR+DMPGLGKEDVK+SVEQN+L I                      IDLPEK+YK D IKAEMKNGVLKV VPK+KEEER NV +VKV+
Sbjct:   70 RDDFLSGNVFDPFSPTRSLSQVLNMMDQFMENPLISAPRAGGLRRGWDARETDDALNLRIDMPGLGKEDVKVSVEQNSLVI--KGEGAKESDDEENARRYSSRIDLPEKMYKTDEIKAEMKNGVLKVVVPKVKEEERANVFHVKVE 213

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BLAST of CD860421 vs. TrEMBL
Match: D3TIC0_MANIN (Small heat shock protein (Fragment) OS=Mangifera indica PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 1.927e-37
Identity = 92/161 (57.14%), Postives = 108/161 (67.08%), Query Frame = 3

Query:    9 SDDRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS---RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKV 482
            SD R++ V R S P  RRD    SDVFDPFSP RSLSQVLN++D +T+NP  +      RRGWDA E E+AL LR+DMPGLGKEDV +SVEQ+TL I                      IDLPEK+YK D IKAEMKNGVLKV VPK+KEEER++V  VK+
Sbjct:   19 SDARDLDVDRRSVPH-RRD--FFSDVFDPFSPTRSLSQVLNLMDQMTENPFFAGTRGGLRRGWDAIEDENALKLRIDMPGLGKEDVNVSVEQSTLVIKGEGAKEADDEESIRRYTSR--IDLPEKMYKTDGIKAEMKNGVLKVVVPKVKEEERSDVFQVKI 174

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BLAST of CD860421 vs. TrEMBL
Match: A5AFX4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022536 PE=3 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.632e-36
Identity = 93/168 (55.36%), Postives = 107/168 (63.69%), Query Frame = 3

Query:   12 DDRNVGVYRHSFPR-TRRDDLL---LSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS------RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            D+R + V R S    +RR D      SDVFDPFSP RSLSQVLN++D   DNP LS +       RR WD +ET+DAL LR+DMPGL KEDVK+SVEQNTLTI                      IDLPEKLYK   IKAEM  GVLK+ VPK+KEEER +VINVKV+
Sbjct:   43 DERRLDVDRLSDRSFSRRGDFAPSSFSDVFDPFSPTRSLSQVLNLMDHFMDNPFLSTSRGMGTGIRRSWDVKETDDALHLRVDMPGLSKEDVKVSVEQNTLTI--QGEEKNETEDEESRRRYSSRIDLPEKLYKTGEIKAEMNKGVLKIVVPKLKEEERTDVINVKVE 208

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BLAST of CD860421 vs. TrEMBL
Match: A8WCV4_GOSHI (Low molecular weight heat shock protein OS=Gossypium hirsutum GN=LMWHSP5 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.635e-36
Identity = 87/168 (51.79%), Postives = 108/168 (64.29%), Query Frame = 3

Query:    6 HSDDRNVGVYRHS---FPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSA---ASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI--XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            + +DR+V V R S     R R      SDVFDPFSP RSLSQVLN++D  TD+P+LS    +SR+GWD RE  +AL++R++MPGL KEDVKISVEQNTL I                        +DLP  +YK+D IKAEMKNGVLKV VPK+KE+ER +V  V V+
Sbjct:   49 NDEDRSVNVQRQSDRSVSRRRDSPRFFSDVFDPFSPTRSLSQVLNLMDQFTDHPLLSTPSLSSRKGWDMREGNNALYIRIEMPGLSKEDVKISVEQNTLIIRGEGGKDWEGEEEEEGGGRRYSSRLDLPPTMYKVDEIKAEMKNGVLKVVVPKVKEDERKDVYQVTVE 216

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BLAST of CD860421 vs. TrEMBL
Match: D7MG30_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492313 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.058e-35
Identity = 83/160 (51.88%), Postives = 106/160 (66.25%), Query Frame = 3

Query:   36 RHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAA-------SRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXX---IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            R S PR R D    SDVFDPFSP RS+SQVLN++D   +NP+LSA        +RRGWD +E +DAL+LR+DMPGL +EDVK+++EQ+TL I                         I LPEK+YKID IKAEMKNGVLKV +PKMKE+ERN+V  ++++
Sbjct:   54 RRSVPRRRGD--FFSDVFDPFSPTRSVSQVLNLMDQFMENPLLSATRGMGASGARRGWDIKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKYEDDDGEEEDQGGNRRFTSRIGLPEKIYKIDEIKAEMKNGVLKVVIPKMKEQERNDVRQIEIN 211

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BLAST of CD860421 vs. TrEMBL
Match: B9R9S7_RICCO (Heat-shock protein, putative OS=Ricinus communis GN=RCOM_1500410 PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 7.579e-34
Identity = 82/161 (50.93%), Postives = 105/161 (65.22%), Query Frame = 3

Query:    9 SDDRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPV--LSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            +++R + V R      RRDD   SDV+DP  P R+LSQVLNM+D + ++P   +     RGWDARETE+AL LR++MPGL KEDVK++VEQNTL I                      IDLPEK+Y+ D IKAEMKNGVLKV VPK+KE ERN+ + +KV+
Sbjct:   46 ANERGIDVDRRRTLPRRRDDFF-SDVWDPIWPGRNLSQVLNMMDRMMESPFRGIGGGLGRGWDARETEEALNLRVEMPGLDKEDVKVTVEQNTLIIKGEGGKESEDEESGRRYAGR--IDLPEKIYRTDQIKAEMKNGVLKVVVPKVKENERNDTVQIKVE 203

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BLAST of CD860421 vs. TrEMBL
Match: D7TN47_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_154.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001315001 PE=4 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 9.898e-34
Identity = 84/164 (51.22%), Postives = 103/164 (62.80%), Query Frame = 3

Query:   15 DRNVGVYRHSFPRTRRDDL-LLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAA------SRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            D N G  R +  R R     L SDVFDPFS  RSLSQVLN++D   +NP+++A+      SRRGWD +E +DALF+R+DMPGLGKEDVK+SVEQNTL I                      IDLP  LYK D IKAEMKNGVLKV VPK+KE+ + +   V ++
Sbjct:   40 DYNDGEDRRTVSRPRYSPSNLFSDVFDPFSRTRSLSQVLNLMDQFMENPLVAASRGMGAVSRRGWDVKEEKDALFVRMDMPGLGKEDVKVSVEQNTLII--KGEGGKELENDETGRKYTSRIDLPANLYKFDEIKAEMKNGVLKVVVPKVKEDGKKDAFQVNIN 201

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BLAST of CD860421 vs. TrEMBL
Match: D7MR07_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495215 PE=4 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.688e-33
Identity = 82/166 (49.40%), Postives = 105/166 (63.25%), Query Frame = 3

Query:   15 DRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS-------RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXX--IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            DRN    RH    +RR     SDVFDPF+P RSLSQ+LN +D +++ P+++A         RRGWD +E ++AL LR+DMPGL +EDVK+++EQNTL I                        I LPEK+YK D IKAEMKNGVLKV +PK+KEEERNNV ++ VD
Sbjct:   50 DRNHNSNRHV---SRRGGDFFSDVFDPFTPTRSLSQMLNFMDQVSEIPLVAATRGMGASGIRRGWDVKEKDEALHLRIDMPGLSREDVKLALEQNTLVIKGEGKTEEGEEGDVSGDGRRFTSRIGLPEKVYKTDEIKAEMKNGVLKVVIPKVKEEERNNVRHINVD 212

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BLAST of CD860421 vs. TrEMBL
Match: O80432_SOLLC (Mitochondrial small heat shock protein OS=Solanum lycopersicum GN=LEMTSHP PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.880e-33
Identity = 80/170 (47.06%), Postives = 111/170 (65.29%), Query Frame = 3

Query:   12 DDRNVGVYRHSFPRTRRDDL---LLSDVFDPFSPP-RSLSQVLNMVDLLTDNPVLSA--------ASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            DDR V V R S     R D    L SDVFDPFSPP RS+SQ+LNM+D + D+P ++A         +RRGWD +E ++AL++++DMPGL KE+VK++VE+NTL I                      +++P+ +YK+D IKAEMKNGVLKV VPK+K+EER +V +VK++
Sbjct:   43 DDRGVDVDRRSDRSVSRRDAFPSLFSDVFDPFSPPIRSVSQLLNMMDQMMDSPFVAAPRAMGAGVGARRGWDVKEDDNALYIKMDMPGLDKENVKVAVEENTLIIKGEGEKESENEEYRRRYSTR--LEIPQNIYKLDGIKAEMKNGVLKVAVPKVKQEERKDVFDVKIE 210

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BLAST of CD860421 vs. SwissProt
Match: HS22M_PEA (Heat shock 22 kDa protein, mitochondrial OS=Pisum sativum GN=HSP22 PE=2 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 6.699e-71
Identity = 138/161 (85.71%), Postives = 138/161 (85.71%), Query Frame = 3

Query:    3 QHSDDRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            QHSDDRNV VYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI                      IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD
Sbjct:   42 QHSDDRNVDVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIKGEEGAKESEEKEKSGRRFSSRIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 202

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BLAST of CD860421 vs. SwissProt
Match: HS23M_ARATH (23.6 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana GN=HSP23.6 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 7.475e-38
Identity = 84/163 (51.53%), Postives = 109/163 (66.87%), Query Frame = 3

Query:   24 VGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAA-------SRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXX--IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            V +YR S PR R D    SDVFDPFSP RS+SQVLN++D   +NP+LSA        +RRGWD +E +DAL+LR+DMPGL +EDVK+++EQ+TL I                        I LP+K+YKID IKAEMKNGVLKV +PKMKE+ERN+V  ++++
Sbjct:   50 VDLYRRSVPRRRGD--FFSDVFDPFSPTRSVSQVLNLMDQFMENPLLSATRGMGASGARRGWDIKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESGNRRFTSRIGLPDKIYKIDEIKAEMKNGVLKVVIPKMKEQERNDVRQIEIN 210

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BLAST of CD860421 vs. SwissProt
Match: HS235_ARATH (23.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana GN=HSP23.5 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 8.553e-34
Identity = 77/164 (46.95%), Postives = 103/164 (62.80%), Query Frame = 3

Query:   15 DRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS-------RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            DRN    RH    +R      S + DPF+P RSLSQ+LN +D +++ P++SA         RRGW+ +E +DAL LR+DMPGL +EDVK+++EQNTL I                      I+LPEK+YK D IKAEMKNGVLKV +PK+KE+ERNN+ ++ VD
Sbjct:   50 DRNHHSNRHV---SRHGGDFFSHILDPFTPTRSLSQMLNFMDQVSEIPLVSATRGMGASGVRRGWNVKEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGEGETEEGEDVSGDGRRFTSRIELPEKVYKTDEIKAEMKNGVLKVVIPKIKEDERNNIRHINVD 210

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BLAST of CD860421 vs. SwissProt
Match: HS23C_CHERU (Small heat shock protein, chloroplastic OS=Chenopodium rubrum GN=HSP23 PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.613e-32
Identity = 80/155 (51.61%), Postives = 97/155 (62.58%), Query Frame = 3

Query:   48 PRTRRDDL---LLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSA------ASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            P +RR D      SDVFDPF   RS+ Q++N++D L +NP ++A      A RRGWD RE E+AL L++DMPGL KEDVK+SVE NTL I                      I+L   LYKID IKAEMKNGVLKVTVPK+KEEE+ +V  V VD
Sbjct:   52 PISRRGDFPASFFSDVFDPFRATRSVGQLMNLMDQLMENPFMAASRGSGRAMRRGWDVREDEEALELKVDMPGLAKEDVKVSVEDNTLII--KSEAEKETEEEEQRRRYSSRIELTPNLYKIDGIKAEMKNGVLKVTVPKIKEEEKKDVFQVMVD 204

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BLAST of CD860421 vs. SwissProt
Match: HS22M_SOYBN (Heat shock 22 kDa protein, mitochondrial OS=Glycine max GN=HSP23.9 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 5.737e-30
Identity = 88/171 (51.46%), Postives = 100/171 (58.48%), Query Frame = 3

Query:    9 SDDRNVGVYRHS---FPRT-RRDDLLLSDV--------FDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            +DD +  + RHS   FP T RRDD+ L  V        F+P S         + V L          S RGWDARETEDAL LR+DMPGL KEDVKISVEQNTL I                      IDLP+KLYKID I+AEMKNGVLKV VPKMKEEER +VI+VKV+
Sbjct:   44 ADDHSTDIDRHSERSFPSTARRDDIFLRCVGSIFSDSEFEPGSEHDGPGHGQS-VPLRVARDRSWRWSGRGWDARETEDALHLRVDMPGLAKEDVKISVEQNTLIIKGEGAKEGDEEESARRYTSR--IDLPDKLYKIDQIRAEMKNGVLKVVVPKMKEEERKDVISVKVE 211

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BLAST of CD860421 vs. SwissProt
Match: HS24M_ORYSJ (24.1 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica GN=HSP24.1 PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 4.546e-27
Identity = 71/164 (43.29%), Postives = 96/164 (58.54%), Query Frame = 3

Query:    3 QHSDDRNVGVYRHSFPRTRRDDLLLSDVF-DPFSPPRSLSQVLNMVDLLTDNPVLSAAS--RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            +  DD   G       R        SDVF DPFS P+SL ++L+++D L      + A+  RRGW+A+E+E+AL LR+DMPGLGKE VK+  EQN+L I                      I+L  ++Y++D IKAEMKNGVLKV VPK+KEE+R +V  V V+
Sbjct:   57 ESDDDSGRGYDTRRPTRDATMPAFFSDVFRDPFSAPQSLGRLLSLMDDLATPAGRAGAATLRRGWNAKESEEALHLRVDMPGLGKEHVKVWAEQNSLVIKGEGEKEAGEDEGAAPARYSGRIELAPEVYRMDQIKAEMKNGVLKVVVPKVKEEQRRDVFQVNVE 220

[Close]

BLAST of CD860421 vs. SwissProt
Match: HS26M_ORYSJ (26.2 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica GN=HSP26.2 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.048e-23
Identity = 63/146 (43.15%), Postives = 85/146 (58.22%), Query Frame = 3

Query:   78 SDVFDPFSPPRSLSQVLNMVDLLT--------DNPVLSAASRRG--WDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            SDV DPF  P SL ++L +++            N + +AA+RRG  W A+E +DA+ L++ MPGLGKE VK+  EQN+L I                      I+LP   +K+D IKAEMKNGVL+V VPK+KEEER +V  V V+
Sbjct:  103 SDVLDPFGAPTSLGRLLALMEDAAVATAAAPGTNGLATAAARRGGWWVAKEDDDAVHLKVSMPGLGKEHVKVWAEQNSLVIKGEGEKDPEDDADAAPPRYTRRIELPADAFKMDKIKAEMKNGVLRVAVPKLKEEERKDVFQVNVE 248

[Close]

BLAST of CD860421 vs. SwissProt
Match: HS25P_ARATH (25.3 kDa heat shock protein, chloroplastic OS=Arabidopsis thaliana GN=HSP25.3 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.340e-15
Identity = 53/145 (36.55%), Postives = 76/145 (52.41%), Query Frame = 3

Query:   84 VFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRG----------WDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI--XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKV 482
            + DP SP R++ Q+L+ +D + ++ +  +   RG          WD +E E  + +R DMPGL KEDVKISVE N L I                        + LP+   K D IKAE+KNGVL +T+PK K E +  VI+V++
Sbjct:   85 LLDPLSPMRTMRQMLDTMDRMFEDTMPVSGRNRGGSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEK-DKIKAELKNGVLFITIPKTKVERK--VIDVQI 226

[Close]

BLAST of CD860421 vs. SwissProt
Match: HS22C_SOYBN (Small heat shock protein, chloroplastic (Fragment) OS=Glycine max GN=HSP22 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.340e-15
Identity = 56/172 (32.56%), Postives = 83/172 (48.26%), Query Frame = 3

Query:    3 QHSDDRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRG-------WDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI-----XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKV 482
            QH    + G      PR    D+    + DP+SP RS+ Q+L+ +D + ++ +       G       WD ++ E  + +R DMPGL KEDVK+SVE + L I                           + LP+   K D +KAE+KNGVL +T+PK K E +  VI+V+V
Sbjct:   12 QHVSKGDQGTAVEKKPRRTAMDISPFGILDPWSPMRSMRQILDTMDRVFEDTMTFPGRNIGGGEIRAPWDIKDEEHEIRMRFDMPGLAKEDVKVSVEDDMLVIKGGHKSEQEHGGDDSWSSRTYSSYDTRLKLPDNCEK-DKVKAELKNGVLYITIPKTKVERK--VIDVQV 180

[Close]

BLAST of CD860421 vs. SwissProt
Match: HS22C_PETHY (Small heat shock protein, chloroplastic OS=Petunia hybrida GN=HSP22 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.981e-14
Identity = 50/155 (32.26%), Postives = 81/155 (52.26%), Query Frame = 3

Query:   48 PRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASR-RG-------WDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI---XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVK 479
            PR    D+    + DP SP R++ Q+++ +D L ++ +    SR RG       WD ++ E+ + +R DMPGL KE+VK+SVE + L I                         + LP+ + K D +KAE+KNGVL +++PK K E++   + +K
Sbjct:   88 PRRMALDVSPFGLLDPMSPMRTMRQMMDTMDRLFEDTMTFPGSRNRGTGEIRAPWDIKDDENEIKMRFDMPGLSKEEVKVSVEDDVLVIKGEHKKEESGKDDSWGRNYSSYDTRLSLPDNVDK-DKVKAELKNGVLLISIPKTKVEKKVTDVEIK 241

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT4G25200.1 (| Symbols: ATHSP23.6-MITO, HSP23.6-MITO | mitochondrion-localized small heat shock protein 23.6 | chr4:12917089-12917858 FORWARD LENGTH=210)

HSP 1 Score: 156.377 bits (394), Expect = 8.283e-39
Identity = 84/163 (51.53%), Postives = 109/163 (66.87%), Query Frame = 3

Query:   24 VGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAA-------SRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXX--IDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            V +YR S PR R D    SDVFDPFSP RS+SQVLN++D   +NP+LSA        +RRGWD +E +DAL+LR+DMPGL +EDVK+++EQ+TL I                        I LP+K+YKID IKAEMKNGVLKV +PKMKE+ERN+V  ++++
Sbjct:   50 VDLYRRSVPRRRGD--FFSDVFDPFSPTRSVSQVLNLMDQFMENPLLSATRGMGASGARRGWDIKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESGNRRFTSRIGLPDKIYKIDEIKAEMKNGVLKVVIPKMKEQERNDVRQIEIN 210

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT5G51440.1 (| Symbols: | HSP20-like chaperones superfamily protein | chr5:20891242-20892013 FORWARD LENGTH=210)

HSP 1 Score: 142.895 bits (359), Expect = 9.476e-35
Identity = 77/164 (46.95%), Postives = 103/164 (62.80%), Query Frame = 3

Query:   15 DRNVGVYRHSFPRTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAAS-------RRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            DRN    RH    +R      S + DPF+P RSLSQ+LN +D +++ P++SA         RRGW+ +E +DAL LR+DMPGL +EDVK+++EQNTL I                      I+LPEK+YK D IKAEMKNGVLKV +PK+KE+ERNN+ ++ VD
Sbjct:   50 DRNHHSNRHV---SRHGGDFFSHILDPFTPTRSLSQMLNFMDQVSEIPLVSATRGMGASGVRRGWNVKEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGEGETEEGEDVSGDGRRFTSRIELPEKVYKTDEIKAEMKNGVLKVVIPKIKEDERNNIRHINVD 210

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT4G27670.1 (| Symbols: HSP21 | heat shock protein 21 | chr4:13819048-13819895 REVERSE LENGTH=227)

HSP 1 Score: 81.6481 bits (200), Expect = 2.593e-16
Identity = 53/145 (36.55%), Postives = 76/145 (52.41%), Query Frame = 3

Query:   84 VFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRG----------WDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI--XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKV 482
            + DP SP R++ Q+L+ +D + ++ +  +   RG          WD +E E  + +R DMPGL KEDVKISVE N L I                        + LP+   K D IKAE+KNGVL +T+PK K E +  VI+V++
Sbjct:   85 LLDPLSPMRTMRQMLDTMDRMFEDTMPVSGRNRGGSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEK-DKIKAELKNGVLFITIPKTKVERK--VIDVQI 226

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT1G52560.2 (| Symbols: | HSP20-like chaperones superfamily protein | chr1:19574783-19575766 REVERSE LENGTH=225)

HSP 1 Score: 53.9138 bits (128), Expect = 5.789e-8
Identity = 47/163 (28.83%), Postives = 72/163 (44.17%), Query Frame = 3

Query:   36 RHSFPRTRRDDLLLSDV-----FDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI--------XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            R +FPR R    L  +      F P     +L Q    ++ + DN  ++     G   +E +D   LR ++PGL KEDVKI+V    LTI                              + LP+   K++ IKAE+KNGVL + +P+  E+ + NV  + V+
Sbjct:   66 RQNFPRRRGRKSLWRNTDDHGYFTPTLNGNTLIQATENMNRIFDNFNVNPFQLMG-QVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDA-KVEDIKAELKNGVLNLVIPR-TEKPKKNVQEISVE 225

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT1G53540.1 (| Symbols: | HSP20-like chaperones superfamily protein | chr1:19980510-19980983 FORWARD LENGTH=157)

HSP 1 Score: 53.9138 bits (128), Expect = 5.789e-8
Identity = 48/142 (33.80%), Postives = 65/142 (45.77%), Query Frame = 3

Query:   51 RTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQ-NTLTI------XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEE 455
            RT   D    DVFDPF    + S + N   +      ++A +    D RET +A   + D+PGL KE+VK+ VE  N L I                              LPE   K++ IKA M+NGVL VTVPK+ E++
Sbjct:   12 RTNVFDPFSLDVFDPFEGFLTPSGLANAPAM-----DVAAFTNAKVDWRETPEAHVFKADLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENA-KMEEIKASMENGVLSVTVPKVPEKK 147

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT1G52560.1 (| Symbols: | HSP20-like chaperones superfamily protein | chr1:19574783-19575766 REVERSE LENGTH=232)

HSP 1 Score: 53.5286 bits (127), Expect = 7.561e-8
Identity = 46/161 (28.57%), Postives = 71/161 (44.10%), Query Frame = 3

Query:   36 RHSFPRTRRDDLLLSDVFDPFSPP---RSLSQVLNMVDLLTDNPVLSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTLTI--------XXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEERNNVINVKVD 485
            R S  R   D    +   + F PP    +L Q    ++ + DN  ++     G   +E +D   LR ++PGL KEDVKI+V    LTI                              + LP+   K++ IKAE+KNGVL + +P+  E+ + NV  + V+
Sbjct:   75 RKSLWRNTDDHGYFTPTLNEFFPPTIGNTLIQATENMNRIFDNFNVNPFQLMG-QVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDA-KVEDIKAELKNGVLNLVIPR-TEKPKKNVQEISVE 232

[Close]

BLAST of CD860421 vs. TAIR peptide
Match: AT3G46230.1 (| Symbols: ATHSP17.4, HSP17.4 | heat shock protein 17.4 | chr3:16984263-16984733 REVERSE LENGTH=156)

HSP 1 Score: 51.9878 bits (123), Expect = 2.200e-7
Identity = 44/144 (30.56%), Postives = 64/144 (44.44%), Query Frame = 3

Query:   51 RTRRDDLLLSDVFDPFSPPRSLSQVLNMVDLLTDNPV--LSAASRRGWDARETEDALFLRLDMPGLGKEDVKISVEQNTL-------TIXXXXXXXXXXXXXXXXXXXXXXIDLPEKLYKIDVIKAEMKNGVLKVTVPKMKEEE 455
            RT   D    DV+DPF           +   LT+ P   ++A +    D RET +A   + D+PGL KE+VK+ VE   +       +                         LPE   K++ +KA M+NGVL VTVPK++E +
Sbjct:   12 RTNVFDPFSLDVWDPFEG--------FLTPGLTNAPAKDVAAFTNAKVDWRETPEAHVFKADVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENA-KVEEVKASMENGVLSVTVPKVQESK 146

The following BLAST results are available for this feature:

BLAST of CD860421 vs. TrEMBL
BLAST of CD860421 vs. SwissProt
BLAST of CD860421 vs. TAIR peptide

BLAST of CD860421 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7ZEP5_PEA	1.492e-61	84.83	Small heat-shock protein OS=Pisum sativum GN=hsp16...	[more]
B9GVE8_POPTR	5.419e-40	62.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D3TIC0_MANIN	1.927e-37	57.14	Small heat shock protein (Fragment) OS=Mangifera i...	[more]
A5AFX4_VITVI	1.632e-36	55.36	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A8WCV4_GOSHI	3.635e-36	51.79	Low molecular weight heat shock protein OS=Gossypi...	[more]
D7MG30_ARALY	1.058e-35	51.88	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9R9S7_RICCO	7.579e-34	50.93	Heat-shock protein, putative OS=Ricinus communis G...	[more]
D7TN47_VITVI	9.898e-34	51.22	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7MR07_ARALY	1.688e-33	49.40	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
O80432_SOLLC	2.880e-33	47.06	Mitochondrial small heat shock protein OS=Solanum ...	[more]

BLAST of CD860421 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
HS22M_PEA	6.699e-71	85.71	Heat shock 22 kDa protein, mitochondrial OS=Pisum ...	[more]
HS23M_ARATH	7.475e-38	51.53	23.6 kDa heat shock protein, mitochondrial OS=Arab...	[more]
HS235_ARATH	8.553e-34	46.95	23.5 kDa heat shock protein, mitochondrial OS=Arab...	[more]
HS23C_CHERU	1.613e-32	51.61	Small heat shock protein, chloroplastic OS=Chenopo...	[more]
HS22M_SOYBN	5.737e-30	51.46	Heat shock 22 kDa protein, mitochondrial OS=Glycin...	[more]
HS24M_ORYSJ	4.546e-27	43.29	24.1 kDa heat shock protein, mitochondrial OS=Oryz...	[more]
HS26M_ORYSJ	1.048e-23	43.15	26.2 kDa heat shock protein, mitochondrial OS=Oryz...	[more]
HS25P_ARATH	2.340e-15	36.55	25.3 kDa heat shock protein, chloroplastic OS=Arab...	[more]
HS22C_SOYBN	2.340e-15	32.56	Small heat shock protein, chloroplastic (Fragment)...	[more]
HS22C_PETHY	1.981e-14	32.26	Small heat shock protein, chloroplastic OS=Petunia...	[more]

BLAST of CD860421 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT4G25200.1	8.283e-39	51.53	\| Symbols: ATHSP23.6-MITO, HSP23.6-MITO \| mitochon...	[more]
AT5G51440.1	9.476e-35	46.95	\| Symbols: \| HSP20-like chaperones superfamily pr...	[more]
AT4G27670.1	2.593e-16	36.55	\| Symbols: HSP21 \| heat shock protein 21 \| chr4:13...	[more]
AT1G52560.2	5.789e-8	28.83	\| Symbols: \| HSP20-like chaperones superfamily pr...	[more]
AT1G53540.1	5.789e-8	33.80	\| Symbols: \| HSP20-like chaperones superfamily pr...	[more]
AT1G52560.1	7.561e-8	28.57	\| Symbols: \| HSP20-like chaperones superfamily pr...	[more]
AT3G46230.1	2.200e-7	30.56	\| Symbols: ATHSP17.4, HSP17.4 \| heat shock protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Heat shock protein Hsp20	PFAM	PF00011	HSP20	coord: 63..160 score: 4.7
IPR002068	Heat shock protein Hsp20	PROFILE	PS01031	HSP20	coord: 63..146 score: 20
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 18..151 score: 1.8
None	No IPR available	GENE3D	2.60.40.790	no description	coord: 31..160 score: 6.4
None	No IPR available	PANTHER	PTHR11527	SMALL HEAT-SHOCK PROTEIN (HSP20) FAMILY	coord: 23..161 score: 1
None	No IPR available	PANTHER	PTHR11527:SF17	HEAT-SHOCK PROTEIN 22	coord: 23..161 score: 1
None	No IPR available	SEG	seg	seg	coord: 97..118 score
None	No IPR available	SEG	seg	seg	coord: 14..29 score
None	No IPR available	SEG	seg	seg	coord: 7..24 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860421 ID=CD860421; Name=CD860421; organism=Pisum sativum; type=EST; length=520bp
ATCAACACTCCGATGACCGTAACGTTGGTGTTTATCGTCACTCCTTCCCT
CGCACTCGCCGCGATGATCTTCTTCTCTCAGATGTGTTCGATCCGTTTTC
TCCCCCACGGAGCTTAAGTCAAGTCCTAAACATGGTGGATTTACTGACGG
ACAATCCGGTCCTTTCTGCTGCTTCGCGACGCGGATGGGATGCGAGAGAG
ACAGAGGATGCTCTGTTTCTCCGTTTGGATATGCCTGGACTCGGTAAAGA
AGACGTGAAGATCTCTGTGGAACAGAACACTCTTACCATTAAAGGTGAAG
AAGGTGCTAAAGAAAGCGAGGAAAAGGAGAAAAGTGGTCGTCGATTCTCG
AGCAGAATTGATTTACCTGAAAAGCTTTACAAGATTGATGTGATTAAAGC
TGAGATGAAAAACGGCGTGCTCAAGGTTACTGTGCCTAAAATGAAGGAAG
AAGAAAGGAACAATGTCATTAACGTCAAGGTTGATTAGAATAGTATCACA
TTTTGCTTCTTTTTCTTTTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR002068	A-crystallin/Hsp20_dom
IPR008978	HSP20-like_chaperone

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