EX569276

EST Overview


Name	EX569276
Unique Name	EX569276
Type	EST
Organism	Pisum sativum (pea)
Sequence length	823

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX569276 vs. TrEMBL
Match: Q9SUS3_ARATH (At4g11380 OS=Arabidopsis thaliana GN=AT4g11380 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: Q9M650_ARATH (Beta-adaptin-like protein B OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: Q8VZF2_ARATH (AT4g23460/F16G20_160 OS=Arabidopsis thaliana GN=At4g23460 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: Q10LA0_ORYSJ (Adapter-related protein complex 1 beta 1 subunit, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os03g23950 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: O81742_ARATH (Beta adaptin-like protein OS=Arabidopsis thaliana GN=F16G20.160 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: D7TV41_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_25.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019391001 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: D7M945_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492527 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: D7LZJ5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489969 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: C5WZQ9_SORBI (Putative uncharacterized protein Sb01g035130 OS=Sorghum bicolor GN=Sb01g035130 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TrEMBL
Match: B9SB76_RICCO (AP-2 complex subunit beta-1, putative OS=Ricinus communis GN=RCOM_0786070 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.981e-14
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP1B_DICDI (AP-1 complex subunit beta OS=Dictyostelium discoideum GN=ap1b1 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.243e-9
Identity = 32/40 (80.00%), Postives = 36/40 (90.00%), Query Frame = -1

Query:    2 NDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 121
            +DSKYF TTKKGEI ELKEEL SQ +DK+K+AVKKVIAAM
Sbjct:    2 SDSKYFQTTKKGEIHELKEELLSQREDKKKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP2B1_RAT (AP-2 complex subunit beta OS=Rattus norvegicus GN=Ap2b1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+T KKGEI ELK ELN++ K+KRK+AVKKVIAAM
Sbjct:    3 DSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP2B1_MOUSE (AP-2 complex subunit beta OS=Mus musculus GN=Ap2b1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+T KKGEI ELK ELN++ K+KRK+AVKKVIAAM
Sbjct:    3 DSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP2B1_HUMAN (AP-2 complex subunit beta OS=Homo sapiens GN=AP2B1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+T KKGEI ELK ELN++ K+KRK+AVKKVIAAM
Sbjct:    3 DSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP2B1_BOVIN (AP-2 complex subunit beta OS=Bos taurus GN=AP2B1 PE=1 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+T KKGEI ELK ELN++ K+KRK+AVKKVIAAM
Sbjct:    3 DSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP1B1_RAT (AP-1 complex subunit beta-1 OS=Rattus norvegicus GN=Ap1b1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+TTKKGEI ELK ELNS  K+K+K+AVKKVIA+M
Sbjct:    3 DSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP1B1_MOUSE (AP-1 complex subunit beta-1 OS=Mus musculus GN=Ap1b1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+TTKKGEI ELK ELNS  K+K+K+AVKKVIA+M
Sbjct:    3 DSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP1B1_HUMAN (AP-1 complex subunit beta-1 OS=Homo sapiens GN=AP1B1 PE=1 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+TTKKGEI ELK ELNS  K+K+K+AVKKVIA+M
Sbjct:    3 DSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASM 41

[Close]

BLAST of EX569276 vs. SwissProt
Match: AP1B1_BOVIN (AP-1 complex subunit beta-1 OS=Bos taurus GN=AP2B1 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.057e-9
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = -1

Query:    2 DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 118
            DSKYF+T KKGEI ELK ELN++ K+KRK+AVKKVIAAM
Sbjct:    3 DSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAM 41

[Close]

BLAST of EX569276 vs. TAIR peptide
Match: AT4G11380.1 (| Symbols: | Adaptin family protein | chr4:6920608-6925444 FORWARD LENGTH=894)

HSP 1 Score: 82.8037 bits (203), Expect = 2.529e-16
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TAIR peptide
Match: AT4G23460.1 (| Symbols: | Adaptin family protein | chr4:12243899-12248898 REVERSE LENGTH=893)

HSP 1 Score: 82.8037 bits (203), Expect = 2.529e-16
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAM 43

[Close]

BLAST of EX569276 vs. TAIR peptide
Match: AT4G11380.2 (| Symbols: | Adaptin family protein | chr4:6920608-6925444 FORWARD LENGTH=916)

HSP 1 Score: 70.0922 bits (170), Expect = 1.696e-12
Identity = 41/65 (63.08%), Postives = 42/65 (64.62%), Query Frame = -1

Query:    2 MSRNDSKYFSTTKKGEIPELKEELNSQYK----------------------DKRKDAVKKVIAAM 130
            MS +DSKYFSTTKKGEIPELKEELNSQYK                      DKRKDAVKKVIAAM
Sbjct:    1 MSGHDSKYFSTTKKGEIPELKEELNSQYKCDDSVHFSVTDWDIVTLVKLRLDKRKDAVKKVIAAM 65

The following BLAST results are available for this feature:

BLAST of EX569276 vs. TrEMBL
BLAST of EX569276 vs. SwissProt
BLAST of EX569276 vs. TAIR peptide

BLAST of EX569276 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SUS3_ARATH	3.981e-14	95.35	At4g11380 OS=Arabidopsis thaliana GN=AT4g11380 PE=...	[more]
Q9M650_ARATH	3.981e-14	95.35	Beta-adaptin-like protein B OS=Arabidopsis thalian...	[more]
Q8VZF2_ARATH	3.981e-14	95.35	AT4g23460/F16G20_160 OS=Arabidopsis thaliana GN=At...	[more]
Q10LA0_ORYSJ	3.981e-14	95.35	Adapter-related protein complex 1 beta 1 subunit, ...	[more]
O81742_ARATH	3.981e-14	95.35	Beta adaptin-like protein OS=Arabidopsis thaliana ...	[more]
D7TV41_VITVI	3.981e-14	95.35	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7M945_ARALY	3.981e-14	95.35	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7LZJ5_ARALY	3.981e-14	95.35	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C5WZQ9_SORBI	3.981e-14	95.35	Putative uncharacterized protein Sb01g035130 OS=So...	[more]
B9SB76_RICCO	3.981e-14	95.35	AP-2 complex subunit beta-1, putative OS=Ricinus c...	[more]

BLAST of EX569276 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
AP1B_DICDI	1.243e-9	80.00	AP-1 complex subunit beta OS=Dictyostelium discoid...	[more]
AP2B1_RAT	8.057e-9	76.92	AP-2 complex subunit beta OS=Rattus norvegicus GN=...	[more]
AP2B1_MOUSE	8.057e-9	76.92	AP-2 complex subunit beta OS=Mus musculus GN=Ap2b1...	[more]
AP2B1_HUMAN	8.057e-9	76.92	AP-2 complex subunit beta OS=Homo sapiens GN=AP2B1...	[more]
AP2B1_BOVIN	8.057e-9	76.92	AP-2 complex subunit beta OS=Bos taurus GN=AP2B1 P...	[more]
AP1B1_RAT	8.057e-9	76.92	AP-1 complex subunit beta-1 OS=Rattus norvegicus G...	[more]
AP1B1_MOUSE	8.057e-9	76.92	AP-1 complex subunit beta-1 OS=Mus musculus GN=Ap1...	[more]
AP1B1_HUMAN	8.057e-9	76.92	AP-1 complex subunit beta-1 OS=Homo sapiens GN=AP1...	[more]
AP1B1_BOVIN	8.057e-9	76.92	AP-1 complex subunit beta-1 OS=Bos taurus GN=AP2B1...	[more]

BLAST of EX569276 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G11380.1	2.529e-16	95.35	\| Symbols: \| Adaptin family protein \| chr4:692060...	[more]
AT4G23460.1	2.529e-16	95.35	\| Symbols: \| Adaptin family protein \| chr4:122438...	[more]
AT4G11380.2	1.696e-12	63.08	\| Symbols: \| Adaptin family protein \| chr4:692060...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 14..25 score
None	No IPR available	SEG	seg	seg	coord: 48..55 score
None	No IPR available	SEG	seg	seg	coord: 2..8 score
None	No IPR available	PANTHER	PTHR11134	ADAPTER-RELATED PROTEIN COMPLEX, BETA SUBUNIT	coord: 21..56 score: 7.2
None	No IPR available	PANTHER	PTHR11134:SF2	ADAPTER-RELATED PROTEIN COMPLEX, BETA SUBUNIT	coord: 21..56 score: 7.2
None	No IPR available	SEG	seg	seg	coord: 1..12 score
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10	no description	coord: 17..56 score: 4.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX569276 ID=EX569276; Name=EX569276; organism=Pisum sativum; type=EST; length=823bp
TCATAGCAGCAATCACTTTTTTCACCGCATCTTTTCTCTTATCCTTGTAC
TGAGAATTAAGCTCTTCTTTGAGCTCAGGGATTTCACCCTTCTTTGTTGT
GGAAAAGTACTTGGAGTCGTTTCTACTCATCTTTCTTGCGCCCTTCTTCT
GCTTCTGGTTTTGCTCGATTCAAATGTCAATGTCGATGGGCGATGCGGAG
AAGGAGAACATCGGTTCTGGATTTGACGATTGTAGAATCAAACAACTTTG
AATTGCTAACAGTTGAATTAGATGGCTTGATAATTGAATGTTGAATAAAT
TCTTGTTTGACAGCAACACCTTCCAATGAATCTACAGGAACAACATTAAC
TACTTTCAAATTCTTTTTTCAGTTATCAACGATTGGCTCTGGCTTTACAA
AACTAAAACTAGCAGTATTTGCGTCACCGGCAGTACCTGTCAGACTTGGC
AAACTAACAAGGGGAGTTGCATTCCCTGAATTTAGTTTAACAGATAATCT
AGAGGGTTCCTCAACATGCTTCTGAATGTTAGATGAAGAAAGACAAAGAG
CAGGTGAATTCACATATTTATACTGCTTCCCATGCAACAGACCTGGAGAA
GTAATGAAATCATTGTTCTGACTCTCTTTGCATGCTTGGGTTATAGATGA
CTCTCTTTGCATGCTTGCGTTATAGATATACCACTTGTTGATGGTGTCAT
CCTAGTGGGGCTCATTAACTGTTTCTCATCATGTGCACTGTGAGTAGTGT
TCAGCCCATCAAAAATTTTAATGGAACCGGCATCACTCCCAGTCTGCGGT
ATGTTCCACATGTCATATATGTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011989	ARM-like

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