GH720114

EST Overview


Name	GH720114
Unique Name	GH720114
Type	EST
Organism	Pisum sativum (pea)
Sequence length	529

Libraries


Library Name	Type
LIBEST_014004 TNE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720114 vs. TrEMBL
Match: Q66PF9_PEA (Monodehydroascorbate reductase I OS=Pisum sativum GN=MDHAR1 PE=4 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 3.577e-66
Identity = 125/127 (98.43%), Postives = 125/127 (98.43%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            DVRRVEHVDHARKSAEQAAK IFAADV KSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI
Sbjct:  307 DVRRVEHVDHARKSAEQAAKVIFAADVRKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 433

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BLAST of GH720114 vs. TrEMBL
Match: D7TJ46_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033815001 PE=4 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.275e-55
Identity = 104/127 (81.89%), Postives = 118/127 (92.91%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI A++ GKSVEEYDYLPYFYSR+FDLSWQFYGDNVGETVLFGDN+PAS K KFGTYWIK+GKVVGAFLEGGTP+EN AIAKVAR +PAVE+++QL +EGL+FA KI
Sbjct:  308 EIRRVEHVDHARKSAEQAVKAIKASEEGKSVEEYDYLPYFYSRAFDLSWQFYGDNVGETVLFGDNNPASPKAKFGTYWIKDGKVVGAFLEGGTPEENTAIAKVARLQPAVENLDQLTKEGLTFACKI 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: A5JPK7_VITVI (Monodehydroascorbate reductase OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 1.665e-55
Identity = 104/127 (81.89%), Postives = 117/127 (92.13%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI A++ GKSVEEYDYLPYFYSR+FDLSWQFYGDNVGETVLFGDN+PAS K KFGTYWIK+GKVVGAFLEGGTP+EN AIAKVAR +PAVE+++QL  EGL+FA KI
Sbjct:  308 EIRRVEHVDHARKSAEQAVKAIKASEEGKSVEEYDYLPYFYSRAFDLSWQFYGDNVGETVLFGDNNPASPKAKFGTYWIKDGKVVGAFLEGGTPEENTAIAKVARLQPAVENLDQLTNEGLTFACKI 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: D7LWS2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487142 PE=4 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.405e-54
Identity = 101/127 (79.53%), Postives = 116/127 (91.34%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI AA+ G S+ EYDYLPYFYSR+FDLSWQFYGDNVGE+VLFGDNDP S KPKFG+YWIKEGKVVGAFLEGGTP+EN AIAK+ARA+P+VE +  L++EGLSFA+KI
Sbjct:  309 EMRRVEHVDHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPKSPKPKFGSYWIKEGKVVGAFLEGGTPEENNAIAKLARAQPSVESLEVLSKEGLSFATKI 435

[Close]

BLAST of GH720114 vs. TrEMBL
Match: B8XF12_ONCHC (Monodehydroascorbate reductase OS=Oncidium Gower Ramsey PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.141e-54
Identity = 100/127 (78.74%), Postives = 116/127 (91.34%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQAAKAI AA+ G S+EEYDYLPYFYSR+FDLSWQFYGDNVG+TVLFGD+DP+S+KPKFGTYWIK+G V+GAFLE GTP+ENKAIAKVAR +P+  D+ QL  EGLSFASK+
Sbjct:  308 ELRRVEHVDHARKSAEQAAKAIKAAEEGISIEEYDYLPYFYSRAFDLSWQFYGDNVGDTVLFGDSDPSSAKPKFGTYWIKDGNVIGAFLESGTPEENKAIAKVARLQPSAVDLEQLKNEGLSFASKL 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: C0LQ98_MALDO (Monodehydroascorbate reductase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 4.102e-54
Identity = 101/127 (79.53%), Postives = 118/127 (92.91%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQ+ KAI A++ GK+VEEYDYLP+FYSRSFDLSWQFYGDNVGETVLFGD++PA+ K KFG+YWIK+GKVVGAFLEGGTP+ENKAIAKVA+A+P V  ++QLA EGLSFASKI
Sbjct:  308 EIRRVEHVDHARKSAEQSVKAIKASEEGKTVEEYDYLPFFYSRSFDLSWQFYGDNVGETVLFGDSNPATPKAKFGSYWIKDGKVVGAFLEGGTPEENKAIAKVAKAQPPVASLDQLATEGLSFASKI 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: A9P7V5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_836268 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.559e-53
Identity = 99/127 (77.95%), Postives = 114/127 (89.76%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            D+RRVEHVDHARKSAEQA KAI + + GK+++ YDYLP+FYSRSFDLSWQFYGDNVG+ VLFGDNDPAS KPKFG+YWIK+GKVVG FLEGGTPDENKAIAKVAR +P VE+++ L +EGLSFA KI
Sbjct:  308 DIRRVEHVDHARKSAEQAVKAIKSNEEGKTIDVYDYLPFFYSRSFDLSWQFYGDNVGDAVLFGDNDPASPKPKFGSYWIKDGKVVGVFLEGGTPDENKAIAKVARVQPPVENLDVLTKEGLSFACKI 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: Q93YG1_MESCR (Monodehydroascorbate reductase OS=Mesembryanthemum crystallinum GN=mdhar1 PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 3.472e-53
Identity = 99/127 (77.95%), Postives = 115/127 (90.55%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI A++ GK+VEEYDYLPYFYSR+FDLSWQFYGDNVG+ VLFGDNDPASS  KFG+YWIK+GKVVGAFLE GTP+ENKAIAKVAR +P  + + QL++EGL+FASKI
Sbjct:  351 ELRRVEHVDHARKSAEQAVKAIKASEEGKAVEEYDYLPYFYSRAFDLSWQFYGDNVGDAVLFGDNDPASSPHKFGSYWIKDGKVVGAFLESGTPEENKAIAKVARVQPPADSLEQLSKEGLTFASKI 477

[Close]

BLAST of GH720114 vs. TrEMBL
Match: B5TV62_CAMSI (Monodehydroascorbate reductase OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.723e-52
Identity = 97/127 (76.38%), Postives = 113/127 (88.98%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAE A KAIFA+  GKS+EEYDYLP+FYSRSF+LSWQFYGDNVG+TVLFGDN P S  PKFG+YWIK+GKVVGAFLE GTP+ENKAIAKVAR +P VE ++ LA++GL+FA KI
Sbjct:  308 EMRRVEHVDHARKSAEHAVKAIFASTEGKSIEEYDYLPFFYSRSFNLSWQFYGDNVGDTVLFGDNSPTSENPKFGSYWIKDGKVVGAFLESGTPEENKAIAKVARVQPPVESLDLLAKDGLTFACKI 434

[Close]

BLAST of GH720114 vs. TrEMBL
Match: Q0WUJ1_ARATH (Monodehydroascorbate reductase (NADH)-like protein OS=Arabidopsis thaliana GN=At5g03630 PE=2 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 2.251e-52
Identity = 98/127 (77.17%), Postives = 114/127 (89.76%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI AA+ G S+ EYDYLPYFYSR+FDLSWQFYGDNVGE+VLFGDNDP S KPKFG+YWIKE KVVGAFLEGG+P+EN AIAK+ARA+P+VE +  L++EGLSFA+ I
Sbjct:  309 EMRRVEHVDHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPESPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR_PEA (Monodehydroascorbate reductase OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 1.137e-68
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI
Sbjct:  307 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 433

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR4_ARATH (Probable monodehydroascorbate reductase, cytoplasmic isoform 4 OS=Arabidopsis thaliana GN=At5g03630 PE=1 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 1.763e-53
Identity = 98/127 (77.17%), Postives = 114/127 (89.76%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI AA+ G S+ EYDYLPYFYSR+FDLSWQFYGDNVGE+VLFGDNDP S KPKFG+YWIKE KVVGAFLEGG+P+EN AIAK+ARA+P+VE +  L++EGLSFA+ I
Sbjct:  309 EMRRVEHVDHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPESPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR_SOLLC (Monodehydroascorbate reductase OS=Solanum lycopersicum GN=AFRR PE=1 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 5.671e-52
Identity = 96/127 (75.59%), Postives = 114/127 (89.76%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDH+RKSAEQA KAIFA++ GKSV+EYDYLPYFYSR+FDLSWQFYGDNVGETVLFGD DP S+  KFG YWIK+GK+VGAFLE G+P+ENKAIAKVA+ +P    ++QLA+EG+SFASKI
Sbjct:  308 EIRRVEHVDHSRKSAEQAVKAIFASEQGKSVDEYDYLPYFYSRAFDLSWQFYGDNVGETVLFGDADPNSATHKFGQYWIKDGKIVGAFLESGSPEENKAIAKVAKVQPPA-TLDQLAQEGISFASKI 433

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDARS_CUCSA (Monodehydroascorbate reductase, seedling isozyme OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 1.263e-51
Identity = 94/127 (74.02%), Postives = 113/127 (88.98%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDH+RKSAEQA KAI A++ GK++EEYDYLPYFYSRSFDLSWQFYGDNVG+ VLFGDN P S+  KFG+YWIK+GKVVGAFLE G+P+ENKAIAKVAR +P+VE  + L +EG+SFASK+
Sbjct:  308 ELRRVEHVDHSRKSAEQAVKAIKASEEGKAIEEYDYLPYFYSRSFDLSWQFYGDNVGDAVLFGDNSPDSATHKFGSYWIKDGKVVGAFLESGSPEENKAIAKVARIQPSVESSDLLLKEGISFASKV 434

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR3_ARATH (Probable monodehydroascorbate reductase, cytoplasmic isoform 3 OS=Arabidopsis thaliana GN=At3g52880 PE=1 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 3.676e-51
Identity = 90/127 (70.87%), Postives = 116/127 (91.34%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            DVRRVEHVDH+RKSAEQA KAI AA+ G +VEEYDYLP+FYSRSFDLSWQFYGDNVG++VLFGD++P++ KP+FG YW++ GKVVGAF+EGG+ DENKA+AKVA+A+P+ E +++L ++G+SFA+KI
Sbjct:  308 DVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 434

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR1_ARATH (Probable monodehydroascorbate reductase, cytoplasmic isoform 1 OS=Arabidopsis thaliana GN=At3g09940 PE=2 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.182e-49
Identity = 91/124 (73.39%), Postives = 109/124 (87.90%), Query Frame = 2

Query:    8 RRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASK 379
            RRVEH D+ARKSA QA KAI A + GK++ +YDYLPYFYSR F LSW+FYG+NVGE+VLFGDNDP S KPKFGTYW+K+GKVVG FLEGGT +E+KAIAKVARA+P+VE ++ L+EEGLSFA+K
Sbjct:  312 RRVEHADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 435

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDARF_CUCSA (Monodehydroascorbate reductase, fruit isozyme (Fragments) OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 6.746e-29
Identity = 70/118 (59.32%), Postives = 80/118 (67.80%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAE 355
            ++RRVEHVDHAR S                +EEYDYLPYFYSR+F+L+WQFYGDNVGETVLF DN        FGTYWI   KVVG FLEGGTPDE     KVAR +P VE ++QLA+
Sbjct:   80 ELRRVEHVDHARLS----------------IEEYDYLPYFYSRTFNLAWQFYGDNVGETVLFPDN--------FGTYWI---KVVGVFLEGGTPDE----YKVARVQPPVESLDQLAK 166

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDAR2_ARATH (Probable monodehydroascorbate reductase, cytoplasmic isoform 2 OS=Arabidopsis thaliana GN=At3g27820 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 7.458e-28
Identity = 65/128 (50.78%), Postives = 86/128 (67.19%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVE-DVNQLAEEGLSFASKI 382
            ++RR+EHVD ARKSA  A  AI   D  K+  ++DYLP+FYSR F  SWQFYGD  G+ V FG+ +   S   FG YW+K+G +VG+FLEGGT +E + I+K  + KPAV  D+ +L  EGL FA  +
Sbjct:  307 EMRRLEHVDSARKSARHAVSAIM--DPIKT-GDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDGKS---FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFAHTV 428

[Close]

BLAST of GH720114 vs. SwissProt
Match: MDARP_ARATH (Monodehydroascorbate reductase, chloroplastic OS=Arabidopsis thaliana GN=At1g63940 PE=2 SV=3)

HSP 1 Score: 107.071 bits (266), Expect = 5.531e-23
Identity = 55/131 (41.98%), Postives = 79/131 (60.31%), Query Frame = 2

Query:   11 RVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLS-------WQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            RVEHVDHAR+SA+   K++  A      + YDYLPYFYSR F+         WQF+GDNVGETV  G+ DP     K  T+WI+ G++ G  +E G+P+E + + K+AR++P V+     +   +  A +I
Sbjct:  365 RVEHVDHARRSAQHCVKSLLTAHT----DTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVEVGNFDP-----KIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEI 486

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT5G03630.1 (| Symbols: ATMDAR2 | Pyridine nucleotide-disulphide oxidoreductase family protein | chr5:922378-924616 REVERSE LENGTH=435)

HSP 1 Score: 208.379 bits (529), Expect = 1.900e-54
Identity = 98/127 (77.17%), Postives = 114/127 (89.76%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            ++RRVEHVDHARKSAEQA KAI AA+ G S+ EYDYLPYFYSR+FDLSWQFYGDNVGE+VLFGDNDP S KPKFG+YWIKE KVVGAFLEGG+P+EN AIAK+ARA+P+VE +  L++EGLSFA+ I
Sbjct:  309 EMRRVEHVDHARKSAEQAVKAIKAAEEGNSIPEYDYLPYFYSRAFDLSWQFYGDNVGESVLFGDNDPESPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKEGLSFATNI 435

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT3G52880.2 (| Symbols: ATMDAR1, MDAR1 | monodehydroascorbate reductase 1 | chr3:19601477-19604366 REVERSE LENGTH=466)

HSP 1 Score: 200.675 bits (509), Expect = 3.962e-52
Identity = 90/127 (70.87%), Postives = 116/127 (91.34%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            DVRRVEHVDH+RKSAEQA KAI AA+ G +VEEYDYLP+FYSRSFDLSWQFYGDNVG++VLFGD++P++ KP+FG YW++ GKVVGAF+EGG+ DENKA+AKVA+A+P+ E +++L ++G+SFA+KI
Sbjct:  340 DVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 466

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT3G52880.1 (| Symbols: ATMDAR1, MDAR1 | monodehydroascorbate reductase 1 | chr3:19601477-19604366 REVERSE LENGTH=434)

HSP 1 Score: 200.675 bits (509), Expect = 3.962e-52
Identity = 90/127 (70.87%), Postives = 116/127 (91.34%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            DVRRVEHVDH+RKSAEQA KAI AA+ G +VEEYDYLP+FYSRSFDLSWQFYGDNVG++VLFGD++P++ KP+FG YW++ GKVVGAF+EGG+ DENKA+AKVA+A+P+ E +++L ++G+SFA+KI
Sbjct:  308 DVRRVEHVDHSRKSAEQAVKAIKAAEGGAAVEEYDYLPFFYSRSFDLSWQFYGDNVGDSVLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQGISFAAKI 434

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT3G09940.2 (| Symbols: MDHAR, ATMDAR3, MDAR3, MDAR2 | monodehydroascorbate reductase | chr3:3056501-3059103 REVERSE LENGTH=433)

HSP 1 Score: 195.667 bits (496), Expect = 1.275e-50
Identity = 91/124 (73.39%), Postives = 109/124 (87.90%), Query Frame = 2

Query:    8 RRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASK 379
            RRVEH D+ARKSA QA KAI A + GK++ +YDYLPYFYSR F LSW+FYG+NVGE+VLFGDNDP S KPKFGTYW+K+GKVVG FLEGGT +E+KAIAKVARA+P+VE ++ L+EEGLSFA+K
Sbjct:  304 RRVEHADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 427

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT3G09940.1 (| Symbols: MDHAR, ATMDAR3, MDAR3, MDAR2 | monodehydroascorbate reductase | chr3:3056501-3059103 REVERSE LENGTH=441)

HSP 1 Score: 195.667 bits (496), Expect = 1.275e-50
Identity = 91/124 (73.39%), Postives = 109/124 (87.90%), Query Frame = 2

Query:    8 RRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASK 379
            RRVEH D+ARKSA QA KAI A + GK++ +YDYLPYFYSR F LSW+FYG+NVGE+VLFGDNDP S KPKFGTYW+K+GKVVG FLEGGT +E+KAIAKVARA+P+VE ++ L+EEGLSFA+K
Sbjct:  312 RRVEHADNARKSAAQAVKAIKAGEEGKTIPDYDYLPYFYSRFFKLSWEFYGENVGESVLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEEGLSFATK 435

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT3G27820.1 (| Symbols: ATMDAR4, MDAR4 | monodehydroascorbate reductase 4 | chr3:10315249-10317881 FORWARD LENGTH=488)

HSP 1 Score: 123.25 bits (308), Expect = 8.040e-29
Identity = 65/128 (50.78%), Postives = 86/128 (67.19%), Query Frame = 2

Query:    2 DVRRVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLSWQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVE-DVNQLAEEGLSFASKI 382
            ++RR+EHVD ARKSA  A  AI   D  K+  ++DYLP+FYSR F  SWQFYGD  G+ V FG+ +   S   FG YW+K+G +VG+FLEGGT +E + I+K  + KPAV  D+ +L  EGL FA  +
Sbjct:  307 EMRRLEHVDSARKSARHAVSAIM--DPIKT-GDFDYLPFFYSRVFAFSWQFYGDPTGDVVHFGEYEDGKS---FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFAHTV 428

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT1G63940.2 (| Symbols: MDAR6 | monodehydroascorbate reductase 6 | chr1:23730095-23733534 FORWARD LENGTH=493)

HSP 1 Score: 107.071 bits (266), Expect = 5.962e-24
Identity = 55/131 (41.98%), Postives = 79/131 (60.31%), Query Frame = 2

Query:   11 RVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLS-------WQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            RVEHVDHAR+SA+   K++  A      + YDYLPYFYSR F+         WQF+GDNVGETV  G+ DP     K  T+WI+ G++ G  +E G+P+E + + K+AR++P V+     +   +  A +I
Sbjct:  365 RVEHVDHARRSAQHCVKSLLTAHT----DTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVEVGNFDP-----KIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEI 486

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT1G63940.1 (| Symbols: MDAR6 | monodehydroascorbate reductase 6 | chr1:23730206-23733534 FORWARD LENGTH=486)

HSP 1 Score: 107.071 bits (266), Expect = 5.962e-24
Identity = 55/131 (41.98%), Postives = 79/131 (60.31%), Query Frame = 2

Query:   11 RVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLS-------WQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            RVEHVDHAR+SA+   K++  A      + YDYLPYFYSR F+         WQF+GDNVGETV  G+ DP     K  T+WI+ G++ G  +E G+P+E + + K+AR++P V+     +   +  A +I
Sbjct:  358 RVEHVDHARRSAQHCVKSLLTAHT----DTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETVEVGNFDP-----KIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEI 479

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT1G63940.4 (| Symbols: MDAR6 | monodehydroascorbate reductase 6 | chr1:23730206-23733534 FORWARD LENGTH=482)

HSP 1 Score: 94.7449 bits (234), Expect = 3.062e-20
Identity = 49/131 (37.40%), Postives = 74/131 (56.49%), Query Frame = 2

Query:   11 RVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLS-------WQFYGDNVGETVLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFASKI 382
            RVEHVDHAR+SA+   K++  A      + YDYLPYFYSR F+         WQF+GDNV         +  +  PK  T+WI+ G++ G  +E G+P+E + + K+AR++P V+     +   +  A +I
Sbjct:  358 RVEHVDHARRSAQHCVKSLLTAHT----DTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNV---------EVGNFDPKIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEI 475

[Close]

BLAST of GH720114 vs. TAIR peptide
Match: AT1G63940.3 (| Symbols: MDAR6 | monodehydroascorbate reductase 6 | chr1:23730206-23732917 FORWARD LENGTH=416)

HSP 1 Score: 61.6178 bits (148), Expect = 2.873e-10
Identity = 30/59 (50.85%), Postives = 37/59 (62.71%), Query Frame = 2

Query:   11 RVEHVDHARKSAEQAAKAIFAADVGKSVEEYDYLPYFYSRSFDLS-------WQFYGDN 166
            RVEHVDHAR+SA+   K++  A      + YDYLPYFYSR F+         WQF+GDN
Sbjct:  358 RVEHVDHARRSAQHCVKSLLTA----HTDTYDYLPYFYSRVFEYEGSPRKVWWQFFGDN 412

The following BLAST results are available for this feature:

BLAST of GH720114 vs. TrEMBL
BLAST of GH720114 vs. SwissProt
BLAST of GH720114 vs. TAIR peptide

BLAST of GH720114 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q66PF9_PEA	3.577e-66	98.43	Monodehydroascorbate reductase I OS=Pisum sativum ...	[more]
D7TJ46_VITVI	1.275e-55	81.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5JPK7_VITVI	1.665e-55	81.89	Monodehydroascorbate reductase OS=Vitis vinifera P...	[more]
D7LWS2_ARALY	2.405e-54	79.53	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B8XF12_ONCHC	3.141e-54	78.74	Monodehydroascorbate reductase OS=Oncidium Gower R...	[more]
C0LQ98_MALDO	4.102e-54	79.53	Monodehydroascorbate reductase OS=Malus domestica ...	[more]
A9P7V5_POPTR	1.559e-53	77.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q93YG1_MESCR	3.472e-53	77.95	Monodehydroascorbate reductase OS=Mesembryanthemum...	[more]
B5TV62_CAMSI	1.723e-52	76.38	Monodehydroascorbate reductase OS=Camellia sinensi...	[more]
Q0WUJ1_ARATH	2.251e-52	77.17	Monodehydroascorbate reductase (NADH)-like protein...	[more]

BLAST of GH720114 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
MDAR_PEA	1.137e-68	100.00	Monodehydroascorbate reductase OS=Pisum sativum PE...	[more]
MDAR4_ARATH	1.763e-53	77.17	Probable monodehydroascorbate reductase, cytoplasm...	[more]
MDAR_SOLLC	5.671e-52	75.59	Monodehydroascorbate reductase OS=Solanum lycopers...	[more]
MDARS_CUCSA	1.263e-51	74.02	Monodehydroascorbate reductase, seedling isozyme O...	[more]
MDAR3_ARATH	3.676e-51	70.87	Probable monodehydroascorbate reductase, cytoplasm...	[more]
MDAR1_ARATH	1.182e-49	73.39	Probable monodehydroascorbate reductase, cytoplasm...	[more]
MDARF_CUCSA	6.746e-29	59.32	Monodehydroascorbate reductase, fruit isozyme (Fra...	[more]
MDAR2_ARATH	7.458e-28	50.78	Probable monodehydroascorbate reductase, cytoplasm...	[more]
MDARP_ARATH	5.531e-23	41.98	Monodehydroascorbate reductase, chloroplastic OS=A...	[more]

BLAST of GH720114 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G03630.1	1.900e-54	77.17	\| Symbols: ATMDAR2 \| Pyridine nucleotide-disulphid...	[more]
AT3G52880.2	3.962e-52	70.87	\| Symbols: ATMDAR1, MDAR1 \| monodehydroascorbate r...	[more]
AT3G52880.1	3.962e-52	70.87	\| Symbols: ATMDAR1, MDAR1 \| monodehydroascorbate r...	[more]
AT3G09940.2	1.275e-50	73.39	\| Symbols: MDHAR, ATMDAR3, MDAR3, MDAR2 \| monodehy...	[more]
AT3G09940.1	1.275e-50	73.39	\| Symbols: MDHAR, ATMDAR3, MDAR3, MDAR2 \| monodehy...	[more]
AT3G27820.1	8.040e-29	50.78	\| Symbols: ATMDAR4, MDAR4 \| monodehydroascorbate r...	[more]
AT1G63940.2	5.962e-24	41.98	\| Symbols: MDAR6 \| monodehydroascorbate reductase ...	[more]
AT1G63940.1	5.962e-24	41.98	\| Symbols: MDAR6 \| monodehydroascorbate reductase ...	[more]
AT1G63940.4	3.062e-20	37.40	\| Symbols: MDAR6 \| monodehydroascorbate reductase ...	[more]
AT1G63940.3	2.873e-10	50.85	\| Symbols: MDAR6 \| monodehydroascorbate reductase ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004099	Pyridine nucleotide-disulphide oxidoreductase, dimerisation	GENE3D	3.30.390.30	no description	coord: 39..118 score: 0.0
IPR016156	FAD/NAD-linked reductase, dimerisation	SUPERFAMILY	55424	FAD/NAD-linked reductases, dimerisation (C-terminal) domain	coord: 30..127 score: 3.4
None	No IPR available	PANTHER	PTHR22912	DISULFIDE OXIDOREDUCTASE	coord: 7..123 score: 4.7
None	No IPR available	PANTHER	PTHR22912:SF31	MONODEHYDROASCORBATE REDUCTASE (NADH)	coord: 7..123 score: 4.7
None	No IPR available	SEG	seg	seg	coord: 51..59 score
None	No IPR available	SEG	seg	seg	coord: 2..20 score
None	No IPR available	SEG	seg	seg	coord: 70..78 score
None	No IPR available	SEG	seg	seg	coord: 42..51 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720114 ID=GH720114; Name=GH720114; organism=Pisum sativum; type=EST; length=529bp
TGACGTGAGAAGAGTTGAACACGTTGATCATGCTCGCAAATCAGCTGAGC
AGGCTGCAAAGGCCATCTTTGCAGCAGATGTAGGAAAATCAGTTGAAGAG
TATGATTACCTTCCATACTTCTATTCCCGTTCGTTTGATCTGTCCTGGCA
ATTCTATGGCGACAATGTTGGTGAGACAGTGCTATTTGGAGACAACGATC
CTGCATCATCAAAGCCTAAATTTGGGACATACTGGATTAAAGAAGGGAAA
GTTGTTGGGGCCTTTTTGGAGGGTGGAACTCCTGATGAGAATAAAGCTAT
TGCCAAAGTTGCAAGAGCCAAGCCTGCAGTGGAGGATGTGAATCAACTTG
CAGAGGAAGGCCTTTCTTTTGCAAGTAAAATTTAATGATATTCTTGGGAA
GGATAAATTGTTTGGTGATTCCACAGAACATATTCGTATTGGTTTCTTTT
TGCTATAGGTTATATCTTAGGGAACTTGTGTTTCTTTGTTTTAAATATGA
TGGTGTGGGATATGCTTTACGCTGTGTAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding

Vocabulary: **Biological Process**
Term	Definition
GO:0045454	cell redox homeostasis
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR004099	Pyr_nucl-diS_OxRdtase_dimer
IPR016156	FAD/NAD-linked_Rdtase_dimer_sf

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