GH720158

EST Overview


Name	GH720158
Unique Name	GH720158
Type	EST
Organism	Pisum sativum (pea)
Sequence length	508

Libraries


Library Name	Type
LIBEST_014004 TNE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720158 vs. TrEMBL
Match: O49931_PEA (Rieske iron-sulfur protein Tic55 OS=Pisum sativum GN=tic55 PE=2 SV=1)

HSP 1 Score: 362.844 bits (930), Expect = 6.280e-99
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 1

Query:    1 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKRE+AQALGFEVT
Sbjct:  114 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREDAQALGFEVT 282

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BLAST of GH720158 vs. TrEMBL
Match: B9RNS2_RICCO (Chlorophyll a oxygenase, putative OS=Ricinus communis GN=RCOM_0920350 PE=4 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 5.514e-87
Identity = 144/168 (85.71%), Postives = 160/168 (95.24%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL V+DK IVL++DG  + +CYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP +AKIP+SACVKTYE+R+SQGV+WVWMSRKTPPN++K+PWFENFARPGFQD STTHELPYDHSILLENLMDPAH+PISHDRTDWSAKRE+AQAL F+VT
Sbjct:  114 DAPLGLTVFDKQIVLYKDGEGELRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPTNAKIPQSACVKTYEIRESQGVVWVWMSRKTPPNLNKLPWFENFARPGFQDTSTTHELPYDHSILLENLMDPAHIPISHDRTDWSAKREDAQALRFKVT 281

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BLAST of GH720158 vs. TrEMBL
Match: D7SV72_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036007001 PE=4 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 7.963e-86
Identity = 143/168 (85.12%), Postives = 156/168 (92.86%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAP+GL V+DK +VL+RDG D  QCYEDRCPHRLAKLSEGQL+DGRLECLYHGWQFEGEGKCVKIPQLPADAKIP+SACVKTYEV+DSQGV+WVWMS +TPPN  K+PWFENFARPGF+D ST HELPYDHSILLENLMDPAHVPISHDRTDWSAKRE+AQ L F+VT
Sbjct:  106 DAPMGLTVFDKQLVLYRDGKDLLQCYEDRCPHRLAKLSEGQLVDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPRSACVKTYEVKDSQGVVWVWMSHRTPPNPDKLPWFENFARPGFRDTSTIHELPYDHSILLENLMDPAHVPISHDRTDWSAKREDAQPLCFKVT 273

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BLAST of GH720158 vs. TrEMBL
Match: B9IL92_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_910872 PE=4 SV=1)

HSP 1 Score: 315.079 bits (806), Expect = 1.502e-84
Identity = 140/168 (83.33%), Postives = 157/168 (93.45%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL V+DK +VL++DG  + +C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPA+AKIP+SACVKTYEV++SQGV+WVWMS KTPPN  K+PWFENFARPGFQD ST HELPYDHSILLENLMDPAH+PISHDRTDW++KRE+AQAL FEVT
Sbjct:  111 DAPLGLNVFDKQLVLYKDGQGELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKIPQSACVKTYEVKESQGVVWVWMSLKTPPNFKKVPWFENFARPGFQDTSTIHELPYDHSILLENLMDPAHIPISHDRTDWTSKREDAQALRFEVT 278

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BLAST of GH720158 vs. TrEMBL
Match: Q9SK50_ARATH (Putative Rieske iron-sulfur protein OS=Arabidopsis thaliana GN=At2g24820 PE=2 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 2.562e-84
Identity = 144/168 (85.71%), Postives = 155/168 (92.26%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL VYD+ IVL++DG    +CYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPA AKIPK+ACVKTYEV+DSQGV+WVWMS KTPPN  K+PWFENFARPGF DISTTHELPYDHSILLENLMDPAHVPISHDRTD++AKRE+AQ L FEVT
Sbjct:  100 DAPLGLTVYDRQIVLYKDGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIPKAACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEVT 267

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BLAST of GH720158 vs. TrEMBL
Match: D7LJF8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_901077 PE=4 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 2.562e-84
Identity = 144/168 (85.71%), Postives = 155/168 (92.26%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL VYD+ IVL++DG    +CYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPA AKIPK+ACVKTYEV+DSQGV+WVWMS KTPPN  K+PWFENFARPGF DISTTHELPYDHSILLENLMDPAHVPISHDRTD++AKRE+AQ L FEVT
Sbjct:  100 DAPLGLTVYDRQIVLYKDGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIPKAACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEVT 267

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BLAST of GH720158 vs. TrEMBL
Match: B9HB88_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1081700 PE=4 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 5.905e-81
Identity = 136/168 (80.95%), Postives = 153/168 (91.07%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL V+DK +VL++DG  + +C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEG CVKIPQLPA+AKIP+SACVKTYEVR+SQGV+WVWMS K+ PN++K+PWFENFARPGF+D S  HELPYDHSILLENLMDPAH+PISHDRTD SAKRE AQ L FEVT
Sbjct:  118 DAPLGLTVFDKQVVLYKDGQGELRCFEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGNCVKIPQLPANAKIPQSACVKTYEVRESQGVVWVWMSSKSQPNINKLPWFENFARPGFKDSSAVHELPYDHSILLENLMDPAHIPISHDRTDLSAKRENAQPLRFEVT 285

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BLAST of GH720158 vs. TrEMBL
Match: C5XUD5_SORBI (Putative uncharacterized protein Sb04g035660 OS=Sorghum bicolor GN=Sb04g035660 PE=4 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 1.079e-74
Identity = 125/168 (74.40%), Postives = 146/168 (86.90%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DA L L V+D+ +VL+RDGN   +C+EDRCPHRLAKLSEGQ++DG+LECLYHGWQF+GEGKCVKIPQLP  AKIP+SAC + YEVRDSQGV+WVW+S   PP+  K+PWFE +AR GF D+ST HELPYDHSILLENLMDPAHVPISHDRTDW+AKRE+AQ L F+VT
Sbjct:  100 DAALPLTVFDRQLVLWRDGNGVLRCHEDRCPHRLAKLSEGQIVDGKLECLYHGWQFDGEGKCVKIPQLPEGAKIPRSACARNYEVRDSQGVVWVWVSDANPPDEGKLPWFEPYARAGFTDLSTVHELPYDHSILLENLMDPAHVPISHDRTDWTAKREDAQPLFFDVT 267

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BLAST of GH720158 vs. TrEMBL
Match: Q6K689_ORYSJ (Os02g0792800 protein OS=Oryza sativa subsp. japonica GN=P0700F06.13 PE=2 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 2.403e-74
Identity = 125/168 (74.40%), Postives = 145/168 (86.31%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DA L L V+D+ +VL+RD     +C+EDRCPHRLAKLSEGQL+DG+LECLYHGWQF+GEGKCVKIPQLP  AKIP++AC ++YEVRDSQGV+W WMS   PP+  K+PWFE +ARPGF D+ST HELPYDHSILLENLMDPAHVPISHDRTDW+AKRE+AQ L FEVT
Sbjct:  101 DAALPLTVFDRQLVLYRDAAGVLRCHEDRCPHRLAKLSEGQLVDGKLECLYHGWQFDGEGKCVKIPQLPDGAKIPRNACARSYEVRDSQGVVWAWMSGTNPPDERKLPWFEPYARPGFTDLSTVHELPYDHSILLENLMDPAHVPISHDRTDWTAKREDAQPLFFEVT 268

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BLAST of GH720158 vs. TrEMBL
Match: A2XAH0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09250 PE=4 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 2.403e-74
Identity = 125/168 (74.40%), Postives = 145/168 (86.31%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DA L L V+D+ +VL+RD     +C+EDRCPHRLAKLSEGQL+DG+LECLYHGWQF+GEGKCVKIPQLP  AKIP++AC ++YEVRDSQGV+W WMS   PP+  K+PWFE +ARPGF D+ST HELPYDHSILLENLMDPAHVPISHDRTDW+AKRE+AQ L FEVT
Sbjct:  101 DAALPLTVFDRQLVLYRDAAGVLRCHEDRCPHRLAKLSEGQLVDGKLECLYHGWQFDGEGKCVKIPQLPDGAKIPRNACARSYEVRDSQGVVWAWMSGTNPPDERKLPWFEPYARPGFTDLSTVHELPYDHSILLENLMDPAHVPISHDRTDWTAKREDAQPLFFEVT 268

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BLAST of GH720158 vs. SwissProt
Match: PAO_ARATH (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 9.980e-24
Identity = 63/179 (35.20%), Postives = 102/179 (56.98%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQ-FQCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPA---DAKIPKS--ACVKTYEVRDSQGVLWVW-----MSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEV 504
            + P   ++  +++VL+ D NDQ +  ++D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ      +A+  KS  AC   +    SQG+L+VW       R       ++P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   + +R+ A+ L F+V
Sbjct:  100 NVPTPFQLLGRDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLP--DDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKV 274

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BLAST of GH720158 vs. SwissProt
Match: CAO_CHLRE (Chlorophyllide a oxygenase, chloroplastic OS=Chlamydomonas reinhardtii GN=CAO PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 1.142e-19
Identity = 50/154 (32.47%), Postives = 78/154 (50.65%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARP--GFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT 456
            D  +  +++ +  V+FRD   Q  C  D C HR   LS G++++G++ C YHGW+F G+G C K+P  P      ++  V      +  G +WVW     P          +FA+P  GF        ++P +H +L+ENL+D AH P +H  T
Sbjct:  317 DTLVPFELFGEPWVMFRDEKGQPSCIRDECAHRGCPLSLGKVVEGQVMCPYHGWEFNGDGACTKMPSTP----FCRNVGVAALPCAEKDGFIWVWPGDGLPAET-----LPDFAQPPEGFLIHAEIMVDVPVEHGLLIENLLDLAHAPFTHTST 461

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BLAST of GH720158 vs. SwissProt
Match: CAO_DUNSA (Chlorophyllide a oxygenase, chloroplastic OS=Dunaliella salina GN=CAO PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.948e-19
Identity = 50/140 (35.71%), Postives = 74/140 (52.86%), Query Frame = 1

Query:   43 VLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDI--STTHELPYDHSILLENLMDPAHVPISHDRT 456
            VLFRD +    C +D C HR   LS G++I+G ++C YHGW+F+G G C K+P      ++     V      +  G +WVW     PP++   P F   A P   D+      ++P +H +L+ENL+D AH P +H  T
Sbjct:  159 VLFRDEHSAPTCIKDSCAHRACPLSLGKVINGHVQCPYHGWEFDGSGACTKMP----STRMCHGVGVAALPCVEKDGFVWVWPGDGPPPDLP--PDFT--APPAGYDVHAEIMVDVPVEHGLLMENLLDLAHAPFTHTTT 290

[Close]

BLAST of GH720158 vs. SwissProt
Match: CAO_ARATH (Chlorophyllide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=CAO PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 2.381e-17
Identity = 46/154 (29.87%), Postives = 81/154 (52.60%), Query Frame = 1

Query:    1 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARP-GFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT 456
            HD  + ++ +++  V+FR  + +  C  + C HR   L  G + +GR++C YHGW++  +G+C K+P      K+ K   +K+    + +G++W+W   + P      P   +   P GF        +LP +H +LL+NL+D AH P +H  T
Sbjct:  232 HDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQCPYHGWEYSTDGECKKMP----STKLLK-VKIKSLPCLEQEGMIWIWPGDEPP-----APILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTHTST 375

[Close]

BLAST of GH720158 vs. SwissProt
Match: CAO_ORYSJ (Chlorophyllide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica GN=CAO PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.543e-16
Identity = 47/153 (30.72%), Postives = 76/153 (49.67%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP-ADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT 456
            D  + +  +++  V+FR  + +  C  + C HR   L  G + +GR++C YHGW++  +GKC K+P     + +I    C       + +G++W+W     P   S IP        GF        ELP +H +LL+NL+D AH P +H  T
Sbjct:  232 DTMVPIDCFEEQWVIFRGKDGRPGCVMNTCAHRACPLHLGSVNEGRIQCPYHGWEYSTDGKCEKMPSTKMLNVRIRSLPCF------EQEGMVWIWPGNDPPK--STIP--SLLPPSGFTIHAEIVMELPVEHGLLLDNLLDLAHAPFTHTST 374

[Close]

BLAST of GH720158 vs. SwissProt
Match: VANA_PSEUH (Vanillate O-demethylase oxygenase subunit OS=Pseudomonas sp. (strain HR199 / DSM 7063) GN=vanA PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.016e-16
Identity = 49/163 (30.06%), Postives = 81/163 (49.69%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRK---TPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEA 483
            D PLG ++ ++ IV +R    +    ED CPHR A LS G + DG+L C YHG +   EGK + +P      ++    C+K+Y V +  G +WVW   +    P  +  + W +N   P +      + +  D+ ++++NLMD  H    H  +    + +EA
Sbjct:   18 DKPLGRQICNEKIVFYRGPEGRVAAVEDFCPHRGAPLSLGFVRDGKLICGYHGLEMGCEGKTLAMP----GQRVQGFPCIKSYAVEERYGFIWVWPGDRELADPALIHHLEWADN---PEWAYGGGLYHIACDYRLMIDNLMDLTHETYVHASSIGQKEIDEA 173

[Close]

BLAST of GH720158 vs. SwissProt
Match: CBAA_COMTE (3-chlorobenzoate-3,4-dioxygenase oxygenase subunit OS=Comamonas testosteroni GN=cbaA PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 7.659e-16
Identity = 38/107 (35.51%), Postives = 63/107 (58.88%), Query Frame = 1

Query:    7 APLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWM-SRKTPPNVSKI 324
            +P+ L +  + +V FR+ +      + RCPHR   L  G++ +G L C+YHGW+F  EGKCV +P +  + +   S  V  Y V++  GV+WV+M +RK  P + ++
Sbjct:   41 SPVRLLLLGEKLVAFREPSGAVGVMDSRCPHRGVSLFMGRVEEGGLRCVYHGWKFSAEGKCVDMPSVRPEDEFKNSVRVARYPVKEMAGVVWVYMGTRKVLPELPRL 147

[Close]

BLAST of GH720158 vs. SwissProt
Match: VANA_PSES9 (Vanillate O-demethylase oxygenase subunit OS=Pseudomonas sp. (strain ATCC 19151) GN=vanA PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.169e-14
Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1

Query:   25 VYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEA 483
            + ++ +V++R    +    ED CPHR A LS G + DG+L C YHG   + +G+   +P      ++    C++ +  ++  G +WVW       + + IP  E    P +      + +  D+ ++++NLMD  H    H  +    + +EA
Sbjct:    2 ICNERMVIYRGAGQRVAALEDFCPHRGAPLSLGSIQDGKLVCGYHGLVMDCDGRTASMPA----QRVQAFPCIRAFPAQERHGFIWVWPGDAALADPALIPHLEWAENPAWAYGGGLYHIACDYRLMIDNLMDLTHETYVHASSIGQKEIDEA 150

[Close]

BLAST of GH720158 vs. SwissProt
Match: PHT3_PSEPU (Phthalate 4,5-dioxygenase oxygenase subunit OS=Pseudomonas putida GN=pht3 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.819e-10
Identity = 38/149 (25.50%), Postives = 73/149 (48.99%), Query Frame = 1

Query:   10 PLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHE---LPYDHSILLENLMDPAHVPISH 447
            P+  +++ +++V+FRD + +    ++ CPHR   L  G+  +  L CLYHGW+ + +G  V++   PA + + +      Y+ R+  G +W +M  +       IP F   A    + +  +     +P + + +LE  +D AH    H
Sbjct:   43 PVRARLFGEDLVVFRDTDGRVGVMDEYCPHRRVSLIYGRNENSGLRCLYHGWKMDVDGNVVEMVSEPAASNMCQKVKHTAYKTREWGGFVWAYMGPQ-----DAIPEFVPPAWAPHEHVRVSIAKAIIPCNWAQILEGAIDSAHSSSLH 186

[Close]

BLAST of GH720158 vs. SwissProt
Match: POBA_PSEPS (Phenoxybenzoate dioxygenase subunit alpha OS=Pseudomonas pseudoalcaligenes GN=pobA PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.387e-9
Identity = 42/160 (26.25%), Postives = 70/160 (43.75%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMS-RKTPPNVSKIPWFENFARPGFQDIST-----------THELPYDHSILLENLMDPAHVPISH 447
            D P  +++  +++VLFRD   +      RC HR   L  G + +  + C YHGW F  +G C+  P  P      ++A    Y V +  G+++ +M   +  P + +    E+     F ++ +              +PY      EN+MDP HV I H
Sbjct:   56 DRPQMVRILGEDLVLFRDKAGRPGLLYPRCMHRGTSLYYGHVEEAGIRCCYHGWLFAVDGTCLNQPCEPEGGLRREAARQPWYPVEERYGLVFAYMGPPEKKPVLPRYDILEDLEEGEFIEVISGGFVSYADHVEDPNVPYHWLQNWENIMDPYHVYILH 215

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT2G24820.1 (| Symbols: TIC55-II | translocon at the inner envelope membrane of chloroplasts 55-II | chr2:10575038-10576829 FORWARD LENGTH=539)

HSP 1 Score: 314.309 bits (804), Expect = 2.286e-86
Identity = 144/168 (85.71%), Postives = 155/168 (92.26%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEVT 507
            DAPLGL VYD+ IVL++DG    +CYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPA AKIPK+ACVKTYEV+DSQGV+WVWMS KTPPN  K+PWFENFARPGF DISTTHELPYDHSILLENLMDPAHVPISHDRTD++AKRE+AQ L FEVT
Sbjct:  100 DAPLGLTVYDRQIVLYKDGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIPKAACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEVT 267

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT4G25650.2 (| Symbols: ACD1-LIKE, PTC52, TIC55-IV | ACD1-like | chr4:13081021-13083153 REVERSE LENGTH=559)

HSP 1 Score: 116.701 bits (291), Expect = 6.997e-27
Identity = 61/155 (39.35%), Postives = 86/155 (55.48%), Query Frame = 1

Query:   10 PLGLKVYDKNIVLFRDGND-QFQCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPADAKIP---KSACVKTYEVRDSQGVLWVWMSR----KTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISH 447
            P G KV   ++V++ D N+ Q++  +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ P D       K ACV  Y       ++W W +     K     +K P+      P F  +    ++PY + +L+ENLMDPAHVP +H
Sbjct:   99 PHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVLVENLMDPAHVPYAH 253

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT4G25650.1 (| Symbols: ACD1-LIKE, PTC52, TIC55-IV | ACD1-like | chr4:13081021-13083153 REVERSE LENGTH=536)

HSP 1 Score: 116.701 bits (291), Expect = 6.997e-27
Identity = 61/155 (39.35%), Postives = 86/155 (55.48%), Query Frame = 1

Query:   10 PLGLKVYDKNIVLFRDGND-QFQCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPADAKIP---KSACVKTYEVRDSQGVLWVWMSR----KTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISH 447
            P G KV   ++V++ D N+ Q++  +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ P D       K ACV  Y       ++W W +     K     +K P+      P F  +    ++PY + +L+ENLMDPAHVP +H
Sbjct:   99 PHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVLVENLMDPAHVPYAH 253

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT3G44880.1 (| Symbols: ACD1, LLS1, PAO | Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain | chr3:16383858-16386204 FORWARD LENGTH=537)

HSP 1 Score: 109.383 bits (272), Expect = 1.117e-24
Identity = 63/179 (35.20%), Postives = 102/179 (56.98%), Query Frame = 1

Query:    4 DAPLGLKVYDKNIVLFRDGNDQ-FQCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPA---DAKIPKS--ACVKTYEVRDSQGVLWVW-----MSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREEAQALGFEV 504
            + P   ++  +++VL+ D NDQ +  ++D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ      +A+  KS  AC   +    SQG+L+VW       R       ++P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   + +R+ A+ L F+V
Sbjct:  100 NVPTPFQLLGRDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLP--DDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKV 274

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT1G44446.3 (| Symbols: CH1, ATCAO, CAO | Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain | chr1:16849132-16851152 REVERSE LENGTH=433)

HSP 1 Score: 89.7373 bits (221), Expect = 9.159e-19
Identity = 49/163 (30.06%), Postives = 84/163 (51.53%), Query Frame = 1

Query:    1 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARP-GFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT---DWSAKR 474
            HD  + ++ +++  V+FR  + +  C  + C HR   L  G + +GR++C YHGW++  +G+C K+P      K+ K   +K+    + +G++W+W   + P      P   +   P GF        +LP +H +LL+NL+D AH P +H  T    WS  R
Sbjct:  232 HDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQCPYHGWEYSTDGECKKMP----STKLLK-VKIKSLPCLEQEGMIWIWPGDEPP-----APILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTHTSTFAKGWSVPR 384

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT1G44446.2 (| Symbols: CH1, ATCAO, CAO | Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain | chr1:16848822-16851152 REVERSE LENGTH=511)

HSP 1 Score: 88.1965 bits (217), Expect = 2.665e-18
Identity = 46/154 (29.87%), Postives = 81/154 (52.60%), Query Frame = 1

Query:    1 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARP-GFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT 456
            HD  + ++ +++  V+FR  + +  C  + C HR   L  G + +GR++C YHGW++  +G+C K+P      K+ K   +K+    + +G++W+W   + P      P   +   P GF        +LP +H +LL+NL+D AH P +H  T
Sbjct:  232 HDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQCPYHGWEYSTDGECKKMP----STKLLK-VKIKSLPCLEQEGMIWIWPGDEPP-----APILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTHTST 375

[Close]

BLAST of GH720158 vs. TAIR peptide
Match: AT1G44446.1 (| Symbols: CH1, ATCAO, CAO | Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain | chr1:16848664-16851152 REVERSE LENGTH=536)

HSP 1 Score: 88.1965 bits (217), Expect = 2.665e-18
Identity = 46/154 (29.87%), Postives = 81/154 (52.60%), Query Frame = 1

Query:    1 HDAPLGLKVYDKNIVLFRDGNDQFQCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQGVLWVWMSRKTPPNVSKIPWFENFARP-GFQ-DISTTHELPYDHSILLENLMDPAHVPISHDRT 456
            HD  + ++ +++  V+FR  + +  C  + C HR   L  G + +GR++C YHGW++  +G+C K+P      K+ K   +K+    + +G++W+W   + P      P   +   P GF        +LP +H +LL+NL+D AH P +H  T
Sbjct:  232 HDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQCPYHGWEYSTDGECKKMP----STKLLK-VKIKSLPCLEQEGMIWIWPGDEPP-----APILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTHTST 375

The following BLAST results are available for this feature:

BLAST of GH720158 vs. TrEMBL
BLAST of GH720158 vs. SwissProt
BLAST of GH720158 vs. TAIR peptide

BLAST of GH720158 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O49931_PEA	6.280e-99	99.41	Rieske iron-sulfur protein Tic55 OS=Pisum sativum ...	[more]
B9RNS2_RICCO	5.514e-87	85.71	Chlorophyll a oxygenase, putative OS=Ricinus commu...	[more]
D7SV72_VITVI	7.963e-86	85.12	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9IL92_POPTR	1.502e-84	83.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SK50_ARATH	2.562e-84	85.71	Putative Rieske iron-sulfur protein OS=Arabidopsis...	[more]
D7LJF8_ARALY	2.562e-84	85.71	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9HB88_POPTR	5.905e-81	80.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5XUD5_SORBI	1.079e-74	74.40	Putative uncharacterized protein Sb04g035660 OS=So...	[more]
Q6K689_ORYSJ	2.403e-74	74.40	Os02g0792800 protein OS=Oryza sativa subsp. japoni...	[more]
A2XAH0_ORYSI	2.403e-74	74.40	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GH720158 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PAO_ARATH	9.980e-24	35.20	Pheophorbide a oxygenase, chloroplastic OS=Arabido...	[more]
CAO_CHLRE	1.142e-19	32.47	Chlorophyllide a oxygenase, chloroplastic OS=Chlam...	[more]
CAO_DUNSA	1.948e-19	35.71	Chlorophyllide a oxygenase, chloroplastic OS=Dunal...	[more]
CAO_ARATH	2.381e-17	29.87	Chlorophyllide a oxygenase, chloroplastic OS=Arabi...	[more]
CAO_ORYSJ	1.543e-16	30.72	Chlorophyllide a oxygenase, chloroplastic OS=Oryza...	[more]
VANA_PSEUH	2.016e-16	30.06	Vanillate O-demethylase oxygenase subunit OS=Pseud...	[more]
CBAA_COMTE	7.659e-16	35.51	3-chlorobenzoate-3,4-dioxygenase oxygenase subunit...	[more]
VANA_PSES9	7.169e-14	25.49	Vanillate O-demethylase oxygenase subunit OS=Pseud...	[more]
PHT3_PSEPU	2.819e-10	25.50	Phthalate 4,5-dioxygenase oxygenase subunit OS=Pse...	[more]
POBA_PSEPS	2.387e-9	26.25	Phenoxybenzoate dioxygenase subunit alpha OS=Pseud...	[more]

BLAST of GH720158 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT2G24820.1	2.286e-86	85.71	\| Symbols: TIC55-II \| translocon at the inner enve...	[more]
AT4G25650.2	6.997e-27	39.35	\| Symbols: ACD1-LIKE, PTC52, TIC55-IV \| ACD1-like ...	[more]
AT4G25650.1	6.997e-27	39.35	\| Symbols: ACD1-LIKE, PTC52, TIC55-IV \| ACD1-like ...	[more]
AT3G44880.1	1.117e-24	35.20	\| Symbols: ACD1, LLS1, PAO \| Pheophorbide a oxygen...	[more]
AT1G44446.3	9.159e-19	30.06	\| Symbols: CH1, ATCAO, CAO \| Pheophorbide a oxygen...	[more]
AT1G44446.2	2.665e-18	29.87	\| Symbols: CH1, ATCAO, CAO \| Pheophorbide a oxygen...	[more]
AT1G44446.1	2.665e-18	29.87	\| Symbols: CH1, ATCAO, CAO \| Pheophorbide a oxygen...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	GENE3D	2.102.10.10	no description	coord: 3..104 score: 8.6
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	PFAM	PF00355	Rieske	coord: 11..80 score: 3.9
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	PROFILE	PS51296	RIESKE	coord: 1..95 score: 29
IPR017941	Rieske [2Fe-2S] iron-sulphur domain	SUPERFAMILY	50022	ISP domain	coord: 2..108 score: 3.3
None	No IPR available	PANTHER	PTHR21266	IRON-SULFUR DOMAIN CONTAINING PROTEIN	coord: 1..169 score: 5
None	No IPR available	PANTHER	PTHR21266:SF19	OXIDASE/CHLOROPHYLL SYNTHASE	coord: 1..169 score: 5
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 90..169 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720158 ID=GH720158; Name=GH720158; organism=Pisum sativum; type=EST; length=508bp
CATGATGCACCATTGGGTCTCAAAGTATATGATAAGAACATTGTGCTGTT
TAGAGATGGTAATGATCAGTTTCAATGCTATGAAGATCGATGTCCTCACA
GGCTCGCAAAACTATCTGAAGGTCAGTTGATTGATGGAAGGCTTGAATGC
CTATATCATGGATGGCAATTCGAAGGAGAGGGAAAATGTGTGAAGATACC
TCAGCTTCCAGCTGATGCCAAAATTCCCAAGTCAGCTTGTGTTAAAACAT
ACGAGGTAAGGGACTCGCAAGGTGTTCTTTGGGTATGGATGTCTCGAAAG
ACACCTCCAAATGTAAGTAAAATACCTTGGTTTGAGAACTTTGCGAGGCC
AGGGTTTCAAGATATTTCAACAACTCATGAACTCCCGTATGATCATTCTA
TTCTGCTGGAAAACTTGATGGATCCTGCTCATGTTCCAATCTCGCACGAT
AGAACGGATTGGTCTGCAAAAAGAGAAGAAGCGCAGGCACTAGGTTTTGA
GGTGACTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR017941	Rieske_2Fe-2S

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