GH720327

EST Overview
NameGH720327
Unique NameGH720327
TypeEST
OrganismPisum sativum (pea)
Sequence length454
Libraries
Library NameType
LIBEST_024270 552Icdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of GH720327 vs. TrEMBL
Match: C6TNI4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.305e-13
Identity = 40/59 (67.80%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  264 MENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATSXXXXXXXXXXXYSVST 440
            MENN S+ISREKIDQ A W+GS VSSAFFSSLERFSCVNVATS           YS +T
Sbjct:    1 MENNDSIISREKIDQAAVWLGSAVSSAFFSSLERFSCVNVATSDPDNDDDDDDYYSTTT 59
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9GL15_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_640301 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.447e-7
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 3 
Query:  255 AATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVAT 389
            A T   N S++S E ++ +A WV +TV SAFFSSLERFSCVNVAT
Sbjct:   11 AITTTTNSSIVSVENLNNVANWVSATVISAFFSSLERFSCVNVAT 55
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9SBE6_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0716650 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.211e-7
Identity = 26/41 (63.41%), Postives = 36/41 (87.80%), Query Frame = 3 
Query:  270 NNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            NN SLIS +K+D++A+WVGS V+SAFF+SLER SC+N+ T+
Sbjct:    7 NNCSLISCDKLDRVASWVGSNVASAFFASLERCSCINLNTA 47
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9RYQ4_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1312560 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.211e-7
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 3 
Query:  273 NGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            N S++S E ++ +A WV +TV SAFFSSLERFSCVN+AT+
Sbjct:   10 NNSIVSMENLNNVANWVSATVISAFFSSLERFSCVNIATN 49
[Close]
BLAST of GH720327 vs. TrEMBL
Match: C6T5G4_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.335e-7
Identity = 29/44 (65.91%), Postives = 30/44 (68.18%), Query Frame = 1 
Query:  247 LIGRRRWRTTAALYRERRSIRWRPGWDPPSHPLSSLPWNAFPAS 378
            LIGRRRWRTT   YR RRS +   G  PPS PLS   WNAFPAS
Sbjct:   14 LIGRRRWRTTTVSYRGRRSTKRPCGSAPPSLPLSFPLWNAFPAS 57
[Close]
BLAST of GH720327 vs. TAIR peptide
Match: AT4G10810.1 (| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G24026.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). | chr4:6645986-6646231 REVERSE LENGTH=81)

HSP 1 Score: 55.0694 bits (131), Expect = 1.953e-8
Identity = 27/45 (60.00%), Postives = 35/45 (77.78%), Query Frame = 3 
Query:  258 ATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            A+  +N S  S EK+DQ A+WV +TV SAFF+SLER SCVN++TS
Sbjct:   11 ASTASNLSTASLEKLDQAASWVSTTVISAFFASLERCSCVNLSTS 55
[Close]
BLAST of GH720327 vs. TAIR peptide
Match: AT4G24026.1 (| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G10810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr4:12486415-12486651 FORWARD LENGTH=78)

HSP 1 Score: 50.447 bits (119), Expect = 4.811e-7
Identity = 23/41 (56.10%), Postives = 31/41 (75.61%), Query Frame = 3 
Query:  270 NNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            N  +  S EKID  A+W+ +TV SAFF+SLER +CVN++TS
Sbjct:   10 NVSTTTSMEKIDHAASWISATVISAFFTSLERCACVNLSTS 50
[Close]
BLAST of GH720327 vs. TAIR peptide
Match: AT4G10810.1 (| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G24026.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). | chr4:6645986-6646231 REVERSE LENGTH=81)

HSP 1 Score: 55.0694 bits (131), Expect = 1.953e-8
Identity = 27/45 (60.00%), Postives = 35/45 (77.78%), Query Frame = 3 
Query:  258 ATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            A+  +N S  S EK+DQ A+WV +TV SAFF+SLER SCVN++TS
Sbjct:   11 ASTASNLSTASLEKLDQAASWVSTTVISAFFASLERCSCVNLSTS 55
[Close]
BLAST of GH720327 vs. TAIR peptide
Match: AT4G24026.1 (| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G10810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr4:12486415-12486651 FORWARD LENGTH=78)

HSP 1 Score: 50.447 bits (119), Expect = 4.811e-7
Identity = 23/41 (56.10%), Postives = 31/41 (75.61%), Query Frame = 3 
Query:  270 NNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            N  +  S EKID  A+W+ +TV SAFF+SLER +CVN++TS
Sbjct:   10 NVSTTTSMEKIDHAASWISATVISAFFTSLERCACVNLSTS 50
[Close]
BLAST of GH720327 vs. TrEMBL
Match: C6TNI4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.882e-13
Identity = 40/59 (67.80%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  264 MENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATSXXXXXXXXXXXYSVST 440
            MENN S+ISREKIDQ A W+GS VSSAFFSSLERFSCVNVATS           YS +T
Sbjct:    1 MENNDSIISREKIDQAAVWLGSAVSSAFFSSLERFSCVNVATSDPDNDDDDDDYYSTTT 59
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9GL15_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_640301 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.810e-7
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 3 
Query:  255 AATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVAT 389
            A T   N S++S E ++ +A WV +TV SAFFSSLERFSCVNVAT
Sbjct:   11 AITTTTNSSIVSVENLNNVANWVSATVISAFFSSLERFSCVNVAT 55
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9SBE6_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0716650 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.265e-7
Identity = 26/41 (63.41%), Postives = 36/41 (87.80%), Query Frame = 3 
Query:  270 NNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            NN SLIS +K+D++A+WVGS V+SAFF+SLER SC+N+ T+
Sbjct:    7 NNCSLISCDKLDRVASWVGSNVASAFFASLERCSCINLNTA 47
[Close]
BLAST of GH720327 vs. TrEMBL
Match: B9RYQ4_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1312560 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.265e-7
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 3 
Query:  273 NGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATS 392
            N S++S E ++ +A WV +TV SAFFSSLERFSCVN+AT+
Sbjct:   10 NNSIVSMENLNNVANWVSATVISAFFSSLERFSCVNIATN 49
[Close]
BLAST of GH720327 vs. Lotus protein
Match: LjSGA_026270.1 (+ phase: 0 )

HSP 1 Score: 84.3445 bits (207), Expect = 1.807e-17
Identity = 45/59 (76.27%), Postives = 47/59 (79.66%), Query Frame = 3 
Query:  264 MENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATSXXXXXXXXXXXYSVST 440
            MENNGS+ISREKIDQMATW+GSTVSSAFFSSLERFSCVNV TS           YSVST
Sbjct:    1 MENNGSIISREKIDQMATWLGSTVSSAFFSSLERFSCVNVTTS--DPDDEDDDDYSVST 57
[Close]
BLAST of GH720327 vs. Soybean peptides
Match: Glyma08g02970.1|PACid:16269760 ()

HSP 1 Score: 86.6557 bits (213), Expect = 8.916e-18
Identity = 45/65 (69.23%), Postives = 48/65 (73.85%), Query Frame = 3 
Query:  246 SDWAATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATSXXXXXXXXXXXYSVST 440
            SD AATMENN S+ISREKIDQ A W+GS VSSAFFSSLERFSCVNVATS           YS +T
Sbjct:   52 SDCAATMENNDSIISREKIDQAAVWLGSAVSSAFFSSLERFSCVNVATSDPDNDDDDDDYYSTTT 116
[Close]
BLAST of GH720327 vs. Soybean peptides
Match: Glyma05g36590.1|PACid:16260925 ()

HSP 1 Score: 78.5666 bits (192), Expect = 2.428e-15
Identity = 40/59 (67.80%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  264 MENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVATSXXXXXXXXXXXYSVST 440
            MENN S+ISREKIDQ A W+GS VSSAFFSSLERFSCVNVATS           YS +T
Sbjct:    1 MENNDSIISREKIDQAAVWLGSAVSSAFFSSLERFSCVNVATSDPDNDEDDDDYYSTTT 59
[Close]
BLAST of GH720327 vs. Soybean peptides
Match: Glyma07g06010.1|PACid:16266356 ()

HSP 1 Score: 52.373 bits (124), Expect = 1.863e-7
Identity = 23/51 (45.10%), Postives = 36/51 (70.59%), Query Frame = 3 
Query:  234 NKFGSDWAATMENNGSLISREKIDQMATWVGSTVSSAFFSSLERFSCVNVA 386
            N   S+  +   NN   +S +K+D++A WVG+ V+SAFF+SLER SC+N++
Sbjct:    8 NNSNSNSNSNSNNNCGFMSCDKLDRVANWVGANVASAFFASLERCSCINLS 58
The following BLAST results are available for this feature:
BLAST of GH720327 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
C6TNI4_SOYBN2.305e-1367.80Putative uncharacterized protein OS=Glycine max PE... [more]
B9GL15_POPTR1.447e-762.22Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SBE6_RICCO4.211e-763.41Putative uncharacterized protein OS=Ricinus commun... [more]
B9RYQ4_RICCO4.211e-762.50Putative uncharacterized protein OS=Ricinus commun... [more]
C6T5G4_SOYBN9.335e-765.91Putative uncharacterized protein (Fragment) OS=Gly... [more]
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BLAST of GH720327 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G10810.11.953e-860.00| Symbols: | unknown protein; FUNCTIONS IN: molec... [more]
AT4G24026.14.811e-756.10| Symbols: | unknown protein; FUNCTIONS IN: molec... [more]
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BLAST of GH720327 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G10810.11.953e-860.00| Symbols: | unknown protein; FUNCTIONS IN: molec... [more]
AT4G24026.14.811e-756.10| Symbols: | unknown protein; FUNCTIONS IN: molec... [more]
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BLAST of GH720327 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 4
Match NameE-valueIdentityDescription
C6TNI4_SOYBN2.882e-1367.80Putative uncharacterized protein OS=Glycine max PE... [more]
B9GL15_POPTR1.810e-762.22Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SBE6_RICCO5.265e-763.41Putative uncharacterized protein OS=Ricinus commun... [more]
B9RYQ4_RICCO5.265e-762.50Putative uncharacterized protein OS=Ricinus commun... [more]
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BLAST of GH720327 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
LjSGA_026270.11.807e-1776.27+ phase: 0 [more]
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BLAST of GH720327 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 3
Match NameE-valueIdentityDescription
Glyma08g02970.1|PACid:162697608.916e-1869.23[more]
Glyma05g36590.1|PACid:162609252.428e-1567.80[more]
Glyma07g06010.1|PACid:162663561.863e-745.10[more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 1..19
score
NoneNo IPR availableSEGsegsegcoord: 49..65
score
NoneNo IPR availableSEGsegsegcoord: 99..109
score
NoneNo IPR availableSEGsegsegcoord: 47..65
score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 1..19
score
NoneNo IPR availableSEGsegsegcoord: 49..65
score
NoneNo IPR availableSEGsegsegcoord: 99..109
score
NoneNo IPR availableSEGsegsegcoord: 47..65
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH720327 ID=GH720327; Name=GH720327; organism=Pisum sativum; type=EST; length=454bp
TAGCGTGGTCGCGGCCGAGGTACAACGCCACCACCACCGTCGACAATCCT
AATTCCTCCGTTCAGAACACAAACGACGTCGCCAACCTCCCTGTTTGAAT
CATCGATACGACATCGTCGTTTCCGGTTCGCTGTATATACCTTTTTTTTT
CTTTTAATACTTTTTATTGTTTCATGTATAAGTATGAGATTTTGATTTCA
AATTTATTAACATGTTTGTGGAACTGCGATGTGAATAAATTTGGATCTGA
TTGGGCGGCGACGATGGAGAACAACGGCAGCCTTATATCGAGAGAGAAGA
TCGATCAGATGGCGACCTGGGTGGGATCCACCGTCTCATCCGCTTTCTTC
TCTTCCTTGGAACGCTTTTCCTGCGTCAACGTCGCTACTTCTGACCCTGA
CAACGACGAAGACGACGACGACGATTACTCCGTCAGTACCTGCCCGGGCG
GCCG
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