FG529301

EST Overview
NameFG529301
Unique NameFG529301
TypeEST
OrganismPisum sativum (pea)
Sequence length608
Libraries
Library NameType
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of FG529301 vs. TrEMBL
Match: B9GGF9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_753357 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.588e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:    5 QSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            QS  +G L  EDGTPD  AI+RLFE+++ D D+ +S+ EL++++K I IG    D +EA  K+++ LD + D  I E EF +G   W+
Sbjct:  432 QSVALGNLIKEDGTPDLLAIRRLFEDIDRDEDNCISKDELKELMKKIEIGKISWDVDEAAEKIMEALDTSGDQMIDEKEFAEGIVSWL 519

HSP 2 Score: 72.7886 bits (177), Expect = 2.191e-11
Identity = 36/88 (40.91%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:    5 QSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDI-HIGPGVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            Q   +G +   DG P+  AI+RLFEE++ DGDD +S +E+ K++ DI   G  ++ + A  +L++ LD+N D +I++ EFV  FTKW+
Sbjct:  295 QERALGIILTGDGAPNINAIQRLFEEIDEDGDDCISPSEVRKLLLDIKSTGMNINKDSASEELIKVLDLNDDKKITKEEFVHTFTKWL 382

HSP 3 Score: 67.0106 bits (162), Expect = 1.202e-9
Identity = 34/64 (53.12%), Postives = 48/64 (75.00%), Query Frame = 1 
Query:  427 TWEDVEKVMEENQSKGVS----ACFEAIAYLVLGITMLSLLAEPLIASVQKFSEAAGISSFFIS 606
            TWE+V+K++++ ++  V     A F+AI  +VLG+ +LS+LAEPLI SVQ FSE AGI SFF+S
Sbjct:  541 TWEEVDKLLKDEKTNAVDKSSWAWFKAIMSMVLGVAILSVLAEPLIHSVQNFSEDAGIPSFFVS 604
[Close]
BLAST of FG529301 vs. TrEMBL
Match: Q5QNI2_ORYSJ (Os01g0212400 protein OS=Oryza sativa subsp. japonica GN=P0031E09.40 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.420e-10
Identity = 32/89 (35.96%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:    2 AQSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            AQ + +G+L NEDGTP+++ IK+LF ++++D    +SR EL  ++  I+      D  +AV K++ D D + ++ + E EFV G  +W+
Sbjct:  290 AQMEALGRLLNEDGTPNEDVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEEAEFVSGMKRWL 378
[Close]
BLAST of FG529301 vs. TrEMBL
Match: B8AAM8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00872 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.420e-10
Identity = 32/89 (35.96%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:    2 AQSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            AQ + +G+L NEDGTP+++ IK+LF ++++D    +SR EL  ++  I+      D  +AV K++ D D + ++ + E EFV G  +W+
Sbjct:  290 AQMEALGRLLNEDGTPNEDVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEEAEFVSGMKRWL 378
[Close]
BLAST of FG529301 vs. TrEMBL
Match: C0P2K9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.855e-10
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:    2 AQSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            AQ   +G+L N+DGTP+++ I++LF ++++D    +SR EL  ++  I+      D  +AV K++ D D ++++ + E EFV G  KW+
Sbjct:  193 AQMQSLGRLLNDDGTPNEQVIRKLFRKIDMDESRSLSRAELHALIVGINFEEVDFDRTDAVDKVMDDFDTSRNDVVEEEEFVQGMRKWL 281
[Close]
BLAST of FG529301 vs. TrEMBL
Match: B6U1M5_MAIZE (Calcium ion binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.855e-10
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:    2 AQSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            AQ   +G+L N+DGTP+++ I++LF ++++D    +SR EL  ++  I+      D  +AV K++ D D ++++ + E EFV G  KW+
Sbjct:  274 AQMQSLGRLLNDDGTPNEQVIRKLFRKIDMDESRSLSRAELHALIVGINFEEVDFDRTDAVDKVMDDFDTSRNDVVEEEEFVQGMRKWL 362
[Close]
BLAST of FG529301 vs. TrEMBL
Match: D0VLU2_9POAL (P20Sh148J07 OS=Saccharum hybrid cultivar R570 GN=Sh148J07_20 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.132e-10
Identity = 30/84 (35.71%), Postives = 55/84 (65.48%), Query Frame = 2 
Query:   17 VGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            +G+L N+DGTP+++ I++LF ++++D    +SR EL  ++  I+      DS +AV K++ D D ++++ + E EFV G  KW+
Sbjct:  277 LGRLLNDDGTPNEDVIRKLFHKIDMDESRSLSRAELHALIVGINFNEVQFDSTDAVDKVMADFDTSRNDIVEEEEFVQGMKKWL 360
[Close]
BLAST of FG529301 vs. TrEMBL
Match: B9NCY8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_790156 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.132e-10
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 2 
Query:    5 QSDMVGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            Q   +G +   +G P+  AI+RLFEE++ D D+ +S+ EL++++K I IG    D +EA  K+++ LD + D  I E EF +G   W+
Sbjct:  229 QERALGSILTGEGAPNINAIQRLFEEIDRDADNCISKDELKELMKKIEIGKISWDVDEAAEKIMEALDTSGDQMIDEKEFAEGIVSWL 316

HSP 2 Score: 67.0106 bits (162), Expect = 1.202e-9
Identity = 34/64 (53.12%), Postives = 48/64 (75.00%), Query Frame = 1 
Query:  427 TWEDVEKVMEENQSKGVS----ACFEAIAYLVLGITMLSLLAEPLIASVQKFSEAAGISSFFIS 606
            TWE+V+K++++ ++  V     A F+AI  +VLG+ +LS+LAEPLI SVQ FSE AGI SFF+S
Sbjct:  338 TWEEVDKLLKDEKTNAVDKSSWAWFKAIMSMVLGVAILSVLAEPLIHSVQNFSEDAGIPSFFVS 401
[Close]
BLAST of FG529301 vs. TrEMBL
Match: D7KKF3_ARALY (Sodium/calcium exchanger family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474446 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 9.206e-10
Identity = 32/84 (38.10%), Postives = 51/84 (60.71%), Query Frame = 2 
Query:   17 VGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            +G+L +++G PD+  I+RLFE ++ + D H+S  EL+ ++  I       D ++AV KLLQD D   D ++ E EFV G   W+
Sbjct:  290 LGRLLDDEGQPDEHVIRRLFETIDANKDGHLSAAELKALIIGISFEEIDFDKDDAVGKLLQDFDKTLDEQVDEEEFVRGIKHWL 373
[Close]
BLAST of FG529301 vs. TrEMBL
Match: D0VLU8_9POAL (P20Sh095F0 OS=Saccharum hybrid cultivar R570 GN=Sh095F04g_20 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.570e-9
Identity = 29/84 (34.52%), Postives = 54/84 (64.29%), Query Frame = 2 
Query:   17 VGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            +G+L N+DGTP+++ I++LF ++++D    +SR EL  ++  I+      D  +AV K++ D D ++++ + E EFV G  KW+
Sbjct:  285 LGRLLNDDGTPNEDVIRKLFHKIDMDESRSLSRAELHALIVGINFNEVEFDRTDAVDKVMADFDTSRNDIVEEEEFVQGMKKWL 368
[Close]
BLAST of FG529301 vs. TrEMBL
Match: A5B4U4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033127 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 4.569e-9
Identity = 31/82 (37.80%), Postives = 49/82 (59.76%), Query Frame = 2 
Query:   17 VGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGPGVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKW 262
            +G+L   +G PD+E IK+LFE +  D D  +S +EL  ++  I I    D +EAV K+  D D   D++I+ + F+ G +KW
Sbjct:  221 LGRLLTANGEPDREVIKKLFEMIGEDSDQQLSASELRALITGIQIKEDSDIDEAVGKVXTDFDACGDSKINLDAFIIGISKW 302
[Close]
BLAST of FG529301 vs. TAIR peptide
Match: AT1G53210.1 (| Symbols: | sodium/calcium exchanger family protein / calcium-binding EF hand family protein | chr1:19844790-19847533 FORWARD LENGTH=585)

HSP 1 Score: 62.3882 bits (150), Expect = 2.191e-10
Identity = 28/84 (33.33%), Postives = 51/84 (60.71%), Query Frame = 2 
Query:   17 VGKLRNEDGTPDKEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGP-GVDSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            +G+L +++G PD+  I++LF  ++ + D H+S  EL+ ++  I       D ++AV K+LQD D   D ++ + EFV G  +W+
Sbjct:  289 LGRLLDDEGQPDEHVIRKLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGIKQWL 372
[Close]
BLAST of FG529301 vs. TAIR peptide
Match: AT1G29025.1 (| Symbols: | Calcium-binding EF-hand family protein | chr1:10124524-10126875 REVERSE LENGTH=578)

HSP 1 Score: 50.447 bits (119), Expect = 8.615e-7
Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:   53 KEAIKRLFEEVNIDGDDHVSRTELEKIVKDIHIGPGV--DSEEAVTKLLQDLDVNKDNEISENEFVDGFTKWI 265
            +E++K LF++ +++ D  +  +EL+ +  D  +   V  D  E  T LL D D N+D EI E EF  G  KW+
Sbjct:  307 QESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDINELATTLLADFDSNRDGEIDETEFAIGIEKWL 379
The following BLAST results are available for this feature:
BLAST of FG529301 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9GGF9_POPTR2.588e-1243.18Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q5QNI2_ORYSJ1.420e-1035.96Os01g0212400 protein OS=Oryza sativa subsp. japoni... [more]
B8AAM8_ORYSI1.420e-1035.96Putative uncharacterized protein OS=Oryza sativa s... [more]
C0P2K9_MAIZE1.855e-1034.83Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
B6U1M5_MAIZE1.855e-1034.83Calcium ion binding protein OS=Zea mays PE=2 SV=1[more]
D0VLU2_9POAL4.132e-1035.71P20Sh148J07 OS=Saccharum hybrid cultivar R570 GN=S... [more]
B9NCY8_POPTR4.132e-1037.50Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7KKF3_ARALY9.206e-1038.10Sodium/calcium exchanger family protein OS=Arabido... [more]
D0VLU8_9POAL1.570e-934.52P20Sh095F0 OS=Saccharum hybrid cultivar R570 GN=Sh... [more]
A5B4U4_VITVI4.569e-937.80Putative uncharacterized protein OS=Vitis vinifera... [more]
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BLAST of FG529301 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT1G53210.12.191e-1033.33| Symbols: | sodium/calcium exchanger family prot... [more]
AT1G29025.18.615e-735.62| Symbols: | Calcium-binding EF-hand family prote... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048Calcium-binding EF-handSMARTSM00054no descriptioncoord: 20..48
score: 3.8coord: 59..87
score:
IPR011992EF-hand-like domainGENE3D1.10.238.10no descriptioncoord: 15..93
score: 5
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 68..80
score
IPR018249EF-HAND 2PROFILEPS50222EF_HAND_2coord: 16..51
score: 10.134coord: 55..90
score: 10
IPR019577SPARC/Testican, calcium-binding domainPFAMPF10591SPARC_Ca_bdgcoord: 7..81
score: 5.6
NoneNo IPR availablePANTHERPTHR10891CALMODULINcoord: 17..89
score: 6.8
NoneNo IPR availableSEGsegsegcoord: 89..101
score
NoneNo IPR availableSUPERFAMILY47473EF-handcoord: 15..89
score: 9.9

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG529301 ID=FG529301; Name=FG529301; organism=Pisum sativum; type=EST; length=608bp
TGCTCAGAGTGATATGGTTGGTAAGCTGCGCAATGAAGACGGTACACCAG
ATAAAGAAGCTATCAAAAGGTTGTTTGAGGAAGTTAATATCGATGGCGAT
GACCATGTTTCGAGAACCGAGTTAGAAAAAATAGTGAAGGACATTCATAT
TGGTCCGGGTGTAGATTCAGAAGAAGCAGTCACAAAACTACTTCAAGATC
TTGATGTCAACAAAGACAATGAAATCAGTGAGAATGAATTTGTGGATGGT
TTCACCAAATGGATTAGCTCAAATTCCAACAAAACTTCTACCTCAAAATC
TTCTCAACATCATCATGAAACTCATCAAGTATGTCCTTATTTTTTCCTTT
ACTCAAATAAGAACTTTTCATATTAAAGTTTAGTCTCTATGAAACTCTTG
ATCAATGAAATGATTGTGGATTATAGACATGGGAAGATGTTGAGAAGGTA
ATGGAAGAGAATCAATCCAAAGGAGTTTCTGCATGTTTTGAAGCTATAGC
ATATTTGGTGTTGGGAATTACTATGCTGTCACTTCTTGCAGAGCCTCTAA
TAGCAAGTGTTCAAAAATTCTCCGAAGCCGCGGGAATTTCATCGTTTTTC
ATCTCATT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0005578proteinaceous extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0007165signal transduction
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR018249EF-HAND 2
IPR019577SPARC/Testican_Ca-bd-dom