FG529477

EST Overview
NameFG529477
Unique NameFG529477
TypeEST
OrganismPisum sativum (pea)
Sequence length486
Libraries
Library NameType
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of FG529477 vs. TrEMBL
Match: D7TQ95_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_40.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025537001 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.757e-13
Identity = 37/41 (90.24%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   MKEKLLYAITEGQGSFHLS
Sbjct: 1447 DLPSVMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFHLS 1487
[Close]
BLAST of FG529477 vs. TrEMBL
Match: B9SGR6_RICCO (Ubiquitin protein ligase E3a, putative OS=Ricinus communis GN=RCOM_0819650 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.757e-13
Identity = 37/41 (90.24%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   MKEKLLYAITEGQGSFHLS
Sbjct: 1521 DLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 1561
[Close]
BLAST of FG529477 vs. TrEMBL
Match: B9MT83_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_739328 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.757e-13
Identity = 37/41 (90.24%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   MKEKLLYAITEGQGSFHLS
Sbjct:  619 DLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 659
[Close]
BLAST of FG529477 vs. TrEMBL
Match: B9IIZ8_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_256737 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.757e-13
Identity = 37/41 (90.24%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   MKEKLLYAITEGQGSFHLS
Sbjct:  610 DLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 650
[Close]
BLAST of FG529477 vs. TrEMBL
Match: Q60EQ3_ORYSJ (Putative uncharacterized protein OJ1280_A04.10 OS=Oryza sativa subsp. japonica GN=OJ1280_A04.10 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.505e-12
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   M+EKLLYAITEGQGSFHLS
Sbjct:  483 DLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 523
[Close]
BLAST of FG529477 vs. TrEMBL
Match: Q0DHI3_ORYSJ (Os05g0463900 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os05g0463900 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.505e-12
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   M+EKLLYAITEGQGSFHLS
Sbjct:  501 DLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 541
[Close]
BLAST of FG529477 vs. TrEMBL
Match: C5YZC1_SORBI (Putative uncharacterized protein Sb09g022820 OS=Sorghum bicolor GN=Sb09g022820 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.505e-12
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   M+EKLLYAITEGQGSFHLS
Sbjct: 1474 DLPSVMTCANYLKLPPYSSKEKMREKLLYAITEGQGSFHLS 1514
[Close]
BLAST of FG529477 vs. TrEMBL
Match: B9FKM6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_18837 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.505e-12
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   M+EKLLYAITEGQGSFHLS
Sbjct: 1022 DLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 1062
[Close]
BLAST of FG529477 vs. TrEMBL
Match: B8AZ12_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_20254 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.505e-12
Identity = 36/41 (87.80%), Postives = 38/41 (92.68%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYSS   M+EKLLYAITEGQGSFHLS
Sbjct: 1311 DLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 1351
[Close]
BLAST of FG529477 vs. TrEMBL
Match: D7M7X1_ARALY (Ubiquitin-protein ligase 4 OS=Arabidopsis lyrata subsp. lyrata GN=UPL4 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.568e-12
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 2 
Query:    2 TDLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            TDLP+VMTCANYLKLP YSS   MKEKL+YAITEGQGSFHLS
Sbjct: 1468 TDLPSVMTCANYLKLPAYSSKEKMKEKLIYAITEGQGSFHLS 1509
[Close]
BLAST of FG529477 vs. SwissProt
Match: UPL4_ARATH (E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis thaliana GN=UPL4 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.185e-14
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 2 
Query:    2 TDLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            TDLP+VMTCANYLKLPPYSS   MKEKL+YAITEGQGSFHLS
Sbjct: 1461 TDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502
[Close]
BLAST of FG529477 vs. SwissProt
Match: UPL3_ARATH (E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis thaliana GN=UPL3 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.915e-10
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYS+   M +KLLYAI EGQGSF LS
Sbjct: 1848 DLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888
[Close]
BLAST of FG529477 vs. SwissProt
Match: TRIPC_HUMAN (Probable E3 ubiquitin-protein ligase TRIP12 OS=Homo sapiens GN=TRIP12 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.992e-8
Identity = 29/40 (72.50%), Postives = 31/40 (77.50%), Query Frame = 2 
Query:    8 LPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            LP+VMTC NYLKLP YSS   M+EKLL A  EGQ SFHLS
Sbjct: 1953 LPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS 1992
[Close]
BLAST of FG529477 vs. SwissProt
Match: YDE1_SCHPO (Probable ubiquitin fusion degradation protein C12B10.01c OS=Schizosaccharomyces pombe GN=SPAC12B10.01c PE=2 SV=2)

HSP 1 Score: 53.9138 bits (128), Expect = 4.414e-7
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 2 
Query:    8 LPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            LP+VMTC NYLKLP YSS   +  +L  AI EGQGSFHLS
Sbjct: 1608 LPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS 1647
[Close]
BLAST of FG529477 vs. TAIR peptide
Match: AT5G02880.1 (| Symbols: UPL4 | ubiquitin-protein ligase 4 | chr5:662643-668847 FORWARD LENGTH=1502)

HSP 1 Score: 78.1814 bits (191), Expect = 2.512e-15
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 2 
Query:    2 TDLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            TDLP+VMTCANYLKLPPYSS   MKEKL+YAITEGQGSFHLS
Sbjct: 1461 TDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502
[Close]
BLAST of FG529477 vs. TAIR peptide
Match: AT4G38600.1 (| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (ubiquitin-transferase) | chr4:18041503-18049292 REVERSE LENGTH=1888)

HSP 1 Score: 65.0846 bits (157), Expect = 2.200e-11
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYS+   M +KLLYAI EGQGSF LS
Sbjct: 1848 DLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888
[Close]
BLAST of FG529477 vs. TAIR peptide
Match: AT4G38600.2 (| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (ubiquitin-transferase) | chr4:18041503-18049073 REVERSE LENGTH=1794)

HSP 1 Score: 65.0846 bits (157), Expect = 2.200e-11
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 2 
Query:    5 DLPNVMTCANYLKLPPYSS**NMKEKLLYAITEGQGSFHLS 127
            DLP+VMTCANYLKLPPYS+   M +KLLYAI EGQGSF LS
Sbjct: 1754 DLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1794
The following BLAST results are available for this feature:
BLAST of FG529477 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7TQ95_VITVI6.757e-1390.24Whole genome shotgun sequence of line PN40024, sca... [more]
B9SGR6_RICCO6.757e-1390.24Ubiquitin protein ligase E3a, putative OS=Ricinus ... [more]
B9MT83_POPTR6.757e-1390.24Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9IIZ8_POPTR6.757e-1390.24Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q60EQ3_ORYSJ1.505e-1287.80Putative uncharacterized protein OJ1280_A04.10 OS=... [more]
Q0DHI3_ORYSJ1.505e-1287.80Os05g0463900 protein (Fragment) OS=Oryza sativa su... [more]
C5YZC1_SORBI1.505e-1287.80Putative uncharacterized protein Sb09g022820 OS=So... [more]
B9FKM6_ORYSJ1.505e-1287.80Putative uncharacterized protein OS=Oryza sativa s... [more]
B8AZ12_ORYSI1.505e-1287.80Putative uncharacterized protein OS=Oryza sativa s... [more]
D7M7X1_ARALY2.568e-1285.71Ubiquitin-protein ligase 4 OS=Arabidopsis lyrata s... [more]
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BLAST of FG529477 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4
Match NameE-valueIdentityDescription
UPL4_ARATH2.185e-1488.10E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis th... [more]
UPL3_ARATH1.915e-1078.05E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis th... [more]
TRIPC_HUMAN3.992e-872.50Probable E3 ubiquitin-protein ligase TRIP12 OS=Hom... [more]
YDE1_SCHPO4.414e-767.50Probable ubiquitin fusion degradation protein C12B... [more]
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BLAST of FG529477 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT5G02880.12.512e-1588.10| Symbols: UPL4 | ubiquitin-protein ligase 4 | chr... [more]
AT4G38600.12.200e-1178.05| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (u... [more]
AT4G38600.22.200e-1178.05| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (u... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG529477 ID=FG529477; Name=FG529477; organism=Pisum sativum; type=EST; length=486bp
CACAGACCTACCTAATGTGATGACCTGCGCCAATTACCTTAAGCTACCTC
CTTATTCGTCATAATAAAACATGAAAGAGAAGCTCTTGTATGCCATTACA
GAGGGTCAAGGATCTTTCCACCTTTCATGACTTCAATCTCTTGACTGGAT
TATTGGATTTTGAAGATGTAATTATTAGGGTAATTGATAGTAATGTAGAT
AAGGCTGTTAACTAGTTGCTGGCAATGATCTCATTGAGTTACAGGAAGTG
CTGGTCACATCAAGTTACTTACTTGGGAACAAAGCTTATTATGTCTTCAC
TGACTACAGTTGTTGGGGGGTGTATATAGCTTGCTGATAGTTAGCTGTTT
CTTTTTTTTAGGGCTGTGTCCTCTGTATAGAAACAGAGGGCAATTTAAGT
GACCATACTTTGATATTTTTTGTACAGTTATTTTGTATTGGGATATCTGC
TGTTACTTTATTATATCATATTTTCCTTGAAATTTC
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