FG529936

EST Overview


Name	FG529936
Unique Name	FG529936
Type	EST
Organism	Pisum sativum (pea)
Sequence length	486

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG529936 vs. TrEMBL
Match: C6T5M3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 2.067e-70
Identity = 136/161 (84.47%), Postives = 145/161 (90.06%), Query Frame = 2

Query:    2 RQQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++QKGEWL+GSFK ENFIPGLVIGFI G L+DLSK  RNHLSKKI SS    Q Q+SVSSNADQELKMVLVVRQDLKM+SGKIASQCAHAATGMYAEL QS+R LLR WEQCGQPKIVVTC+NQQEMNKL E AES+GLPTFVVADAGRTQVSAGSKTVLA
Sbjct:   17 QKQKGEWLAGSFKAENFIPGLVIGFIFGLLLDLSKSGRNHLSKKICSSARPQQQQISVSSNADQELKMVLVVRQDLKMKSGKIASQCAHAATGMYAELMQSDRYLLRRWEQCGQPKIVVTCRNQQEMNKLAEAAESIGLPTFVVADAGRTQVSAGSKTVLA 177

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BLAST of FG529936 vs. TrEMBL
Match: D7SU93_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035616001 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.622e-60
Identity = 118/161 (73.29%), Postives = 132/161 (81.99%), Query Frame = 2

Query:    2 RQQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++Q+ EW + +FKPENFIPGL+IGFI GF +DLSK  +N           + Q Q  VSSN+D+ELKMVLVVRQDLKM +GKIASQCAHAATGMYAEL QS RSLLR WEQCGQ KIVVTCKNQQEMNKLKE AES+GLPTFVVADAGRTQV +GSKTVLA
Sbjct:   14 QKQEREWFAVNFKPENFIPGLIIGFIFGFFLDLSKVPKNQPRHNNSLLGKRQQQQQLVSSNSDEELKMVLVVRQDLKMGTGKIASQCAHAATGMYAELVQSQRSLLRQWEQCGQAKIVVTCKNQQEMNKLKEAAESIGLPTFVVADAGRTQVLSGSKTVLA 174

[Close]

BLAST of FG529936 vs. TrEMBL
Match: B9RRZ0_RICCO (Peptidyl-tRNA hydrolase, putative OS=Ricinus communis GN=RCOM_0799890 PE=4 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.101e-55
Identity = 114/161 (70.81%), Postives = 129/161 (80.12%), Query Frame = 2

Query:    2 RQQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            +Q++   L+ SF+ ENFIPGLVIGFI G  +DL+ P +N   KK FS   K Q Q  VS N D ELKMVLVVRQDLKM +GKIASQCAHAATGMY+EL QS + LL  WEQCGQPKIVVTC+NQQEMNKL+E A+S+GLPTFVVADAGRTQV AGSKTVLA
Sbjct:   18 KQEREGGLTESFRLENFIPGLVIGFIFGLFLDLTNPIKNSFKKKNFSG--KSQQQSLVSYNGDPELKMVLVVRQDLKMGAGKIASQCAHAATGMYSELMQSQQLLLSRWEQCGQPKIVVTCRNQQEMNKLQEAADSIGLPTFVVADAGRTQVLAGSKTVLA 176

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BLAST of FG529936 vs. TrEMBL
Match: A9PDF5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_561744 PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 5.464e-55
Identity = 112/161 (69.57%), Postives = 128/161 (79.50%), Query Frame = 2

Query:    2 RQQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            +Q+KG  L+ SF+PENFIPGL+IGFI G  +DLS P++N   KK F      Q  L    + D+ELKMV VVRQDLKM +GKIASQCAHAATGMYAEL  S+R LLR WEQ GQPKIVVTCKNQQEMNK++E AE++GLPTF VADAGRTQVSAGSKTVLA
Sbjct:   15 KQEKGG-LAESFRPENFIPGLIIGFIFGLFLDLSNPSKNQTKKKNFLPGKSQQQSLE---DGDKELKMVFVVRQDLKMGAGKIASQCAHAATGMYAELIYSHRILLRQWEQSGQPKIVVTCKNQQEMNKIREAAENIGLPTFAVADAGRTQVSAGSKTVLA 171

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BLAST of FG529936 vs. TrEMBL
Match: Q3E6Z5_ARATH (Putative uncharacterized protein At4g32900.2 OS=Arabidopsis thaliana GN=At4g32900 PE=4 SV=2)

HSP 1 Score: 209.149 bits (531), Expect = 1.139e-52
Identity = 111/161 (68.94%), Postives = 132/161 (81.99%), Query Frame = 2

Query:    5 QQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNA-DQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            +Q+ + +S SF+ EN IPG+VIGFI G ++DLS+   + + +    S+ K+Q Q SV  N  DQELKMVLVVRQDLKMR+GKIASQCAHAATGMYAEL QS+R LLR WE+ GQPKIVVTCKNQQEMNK+ E AESVGLPTFVVADAGRT+V+AGS+TVLA
Sbjct:   15 RQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLLSS-KVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQQEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLA 174

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BLAST of FG529936 vs. TrEMBL
Match: Q6F2Z0_ORYSJ (Os05g0520200 protein OS=Oryza sativa subsp. japonica GN=P0483D07.6 PE=4 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 8.449e-48
Identity = 105/169 (62.13%), Postives = 126/169 (74.56%), Query Frame = 2

Query:    5 QQKGE--WLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNN--KLQHQLSVSSNAD-----QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++KGE  WL+ S +P NF+PGL IGF+ G L+DL+   R   S  +  +    +   +   SS+A      +ELKMVLVVR+DLKMR+GKIASQCAHAATG+YAEL  SNR LLR WEQ GQ KIV+TCKNQQEMN++KETAE  G+PTFVVADAGRTQV AGSKTVLA
Sbjct:   17 RRKGEDPWLAASLRPGNFLPGLAIGFLLGLLVDLTSSWRPQSSPPLAPAAPAPRGSKRTGGSSSAGGGIGGEELKMVLVVRKDLKMRAGKIASQCAHAATGLYAELMASNRGLLRQWEQFGQAKIVLTCKNQQEMNRIKETAEHRGIPTFVVADAGRTQVQAGSKTVLA 185

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BLAST of FG529936 vs. TrEMBL
Match: A2Y6L9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_20660 PE=4 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 8.449e-48
Identity = 105/169 (62.13%), Postives = 126/169 (74.56%), Query Frame = 2

Query:    5 QQKGE--WLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNN--KLQHQLSVSSNAD-----QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++KGE  WL+ S +P NF+PGL IGF+ G L+DL+   R   S  +  +    +   +   SS+A      +ELKMVLVVR+DLKMR+GKIASQCAHAATG+YAEL  SNR LLR WEQ GQ KIV+TCKNQQEMN++KETAE  G+PTFVVADAGRTQV AGSKTVLA
Sbjct:   17 RRKGEDPWLAASLRPGNFLPGLAIGFLLGLLVDLTSSWRPQSSPPLAPAAPAPRGSKRTGGSSSAGGGIGGEELKMVLVVRKDLKMRAGKIASQCAHAATGLYAELMASNRGLLRQWEQFGQAKIVLTCKNQQEMNRIKETAEHRGIPTFVVADAGRTQVQAGSKTVLA 185

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BLAST of FG529936 vs. TrEMBL
Match: C5Z1B8_SORBI (Putative uncharacterized protein Sb09g025940 OS=Sorghum bicolor GN=Sb09g025940 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.441e-47
Identity = 105/168 (62.50%), Postives = 124/168 (73.81%), Query Frame = 2

Query:    5 QQKGE--WLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKK----IFSSNNKLQHQLSVSSNAD--QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++KGE  WL+ S +P NF+PGL IGF+ G L+DLS   R   S        S+      + S SS A   +ELKMVLVVRQDLKM +GKIASQCAHAATG+Y+EL  SNR LL+ WEQ GQ KIV+TCKNQQEMN+LKETA+  G+PTF+VADAGRTQV AGSKTVLA
Sbjct:   15 RRKGEEPWLAASLRPANFLPGLAIGFLLGVLLDLSSSWRPKSSPAPAPAAASARGSSSKRASGSSFASGGEELKMVLVVRQDLKMGAGKIASQCAHAATGLYSELLSSNRGLLKQWEQLGQAKIVLTCKNQQEMNRLKETAQHRGIPTFIVADAGRTQVLAGSKTVLA 182

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BLAST of FG529936 vs. TrEMBL
Match: A9P0R8_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.342e-47
Identity = 96/160 (60.00%), Postives = 118/160 (73.75%), Query Frame = 2

Query:    5 QQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++ G W+SGS KPE+F+PGL IGF+ G L+ L    R   +    ++       +    N+D E+KMV VVRQDLKM +GKIASQCAHAATG+Y  L +S ++LL  WEQCGQPKIVVTCKNQQEMNKL+  AE +GL TF+VADAGRTQVSAGS+TVLA
Sbjct:   16 RKPGAWISGSIKPESFLPGLAIGFLLGLLVKLPNNFRRITNTGAKTARVNSTRPVRSDGNSD-EVKMVFVVRQDLKMGAGKIASQCAHAATGIYGALMRSQQALLNKWEQCGQPKIVVTCKNQQEMNKLQAVAEKIGLLTFIVADAGRTQVSAGSRTVLA 174

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BLAST of FG529936 vs. TrEMBL
Match: A9TAP2_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_220405 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.000e-40
Identity = 87/163 (53.37%), Postives = 113/163 (69.33%), Query Frame = 2

Query:    2 RQQKGE-WLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNAD--QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            R++K + WL+ +  P + +PG+V+GF+ G  ++L K       +        +    SVS   D  +ELKMV VVRQDLKM  GKIA+QCAHAA G+Y EL   NR+LL+ WE CGQPKIVVTC+NQ+EMN+L+  A+  GLPT+ VADAGRTQV+AGSKTVL
Sbjct:   16 REKKTQPWLAKALNPASLLPGMVVGFLLGLWVELPKRGFGSSRQNPVPKKKGIVGNQSVSQIGDDGEELKMVFVVRQDLKMGGGKIAAQCAHAAVGIYNELLTRNRALLKRWEDCGQPKIVVTCRNQKEMNELRGKADGAGLPTYTVADAGRTQVAAGSKTVL 178

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BLAST of FG529936 vs. SwissProt
Match: PTH2_HUMAN (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 3.364e-23
Identity = 56/142 (39.44%), Postives = 82/142 (57.75%), Query Frame = 2

Query:   59 GLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNADQ-ELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            GL +G  CG  +  S      +  K  +S      +   S   D  E KM+LVVR DLKM  GK+A+QC+HAA   Y ++ + N  +L+ WE CGQPK+VV   +++ +  L   A+ +GL   ++ DAGRTQ++ GS+TVL
Sbjct:   18 GLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVL 159

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BLAST of FG529936 vs. SwissProt
Match: PTH2_BOVIN (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 3.719e-22
Identity = 55/142 (38.73%), Postives = 84/142 (59.15%), Query Frame = 2

Query:   62 LVIGFICGFLIDLSKPTRNHLSKK--IFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            L  G  CG  +      R  +  K  +  +N   + + S+   +  E KM+LVVR DLKM  GK+A+QC+HAA   Y ++ + N  LL+ WE CGQPK+VV   +++ + +L   A+ +GL   ++ DAGRTQ++ GS+TVL
Sbjct:   19 LAAGVACGVCLGWGLRMRFGMLPKSSVRETNPDTETEASILGESG-EYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEYCGQPKVVVKAPDEETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVL 159

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BLAST of FG529936 vs. SwissProt
Match: PTH2_MOUSE (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus GN=Ptrh2 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.148e-21
Identity = 54/145 (37.24%), Postives = 81/145 (55.86%), Query Frame = 2

Query:   62 LVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNAD-----QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            L  G  CG  +      R+HL     +S ++        + A       E KM+LVVR DLKM  GK+A+QC+HAA   Y +  + +  +L+ WE CGQPK+VV   ++  + +L   A+++GL   ++ DAGRTQ+  GS+TVL
Sbjct:   19 LAAGVACGMCLGWG--LRSHLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVL 161

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BLAST of FG529936 vs. SwissProt
Match: PTH2_DROME (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster GN=CG1307 PE=1 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 4.546e-20
Identity = 50/99 (50.51%), Postives = 61/99 (61.62%), Query Frame = 2

Query:  188 DQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            +   KMVLVVR DLKM  GKIA+QC H A G Y         LLR WE CG  KI V  +++ E+  +K+ AE   L T ++ DAGRTQ+ A SKTVLA
Sbjct:   69 NDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVESEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLA 167

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BLAST of FG529936 vs. SwissProt
Match: PTH2_SCHPO (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 5.938e-20
Identity = 62/172 (36.05%), Postives = 90/172 (52.33%), Query Frame = 2

Query:   41 PENFIPGLVIGFICGFLIDLSKPTRNHLSKKIF-SSNNKLQHQLSVSSNADQE------------------------LKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            P   + G V+GF+ G    ++  +R + SK    +SN + +  +SVS++ D E                         KMVLVVR DL M  GKIA+QCAHAA   Y   +  +  L+R WE  GQ KI +  + ++ +  L+  A S+GL   V+ DAGRTQ+++GS TVL
Sbjct:   14 PAAVLVGAVVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESETELLDMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLVRIWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVL 185

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BLAST of FG529936 vs. SwissProt
Match: PTH_SULTO (Peptidyl-tRNA hydrolase OS=Sulfolobus tokodaii GN=pth PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.159e-15
Identity = 45/98 (45.92%), Postives = 61/98 (62.24%), Query Frame = 2

Query:  200 KMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSL----LRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            KMV+VVR DL M  GKIA+Q AHAA  +  ++  S+ SL    L  W + GQPKIVV   +  E+NK  E A  + LPT ++ DAG+TQ+  G+ T +
Sbjct:    4 KMVIVVRTDLDMGKGKIAAQVAHAAVSLVLDILNSSNSLWKEWLYKWLEEGQPKIVVKVPSLDELNKRYEKAIELNLPTTIIQDAGKTQIEPGTITCI 101

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BLAST of FG529936 vs. SwissProt
Match: PTH_PYRFU (Peptidyl-tRNA hydrolase OS=Pyrococcus furiosus GN=pth PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.159e-15
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 2

Query:  200 KMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            K V+V R DLK+  GK+A Q AH A     E  +  R     W + GQ K+VV  +N++E+ KLK  AE +GLPT ++ DAG T++  G+ TVLA
Sbjct:    6 KQVIVARSDLKLSKGKLAVQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKAQNEEELFKLKSEAEKLGLPTALIRDAGLTEIPPGTITVLA 100

[Close]

BLAST of FG529936 vs. SwissProt
Match: PTH2_DICDI (Probable peptidyl-tRNA hydrolase 2 OS=Dictyostelium discoideum GN=pth2 PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.513e-15
Identity = 38/97 (39.18%), Postives = 62/97 (63.92%), Query Frame = 2

Query:  194 ELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            + K VLV+R DL M  GKI +QC HA+ G   +  + ++ +LR WE  G  KI +   +++E+  L+  A+ +G+ +++V D+G TQ+ +GS TVLA
Sbjct:   95 DCKQVLVIRTDLGMTKGKIIAQCCHASLGARQKALKDSKKVLRQWENFGCAKITLKANSKEELVDLRNKAKELGVVSYLVLDSGHTQIPSGSATVLA 191

[Close]

BLAST of FG529936 vs. SwissProt
Match: PTH2_YEAST (Peptidyl-tRNA hydrolase 2 OS=Saccharomyces cerevisiae GN=PTH2 PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.977e-15
Identity = 43/102 (42.16%), Postives = 62/102 (60.78%), Query Frame = 2

Query:  194 ELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQS------NRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVL 481
            E++M LV+RQDL M  GKIA+QC HAA   +  +  +      N  + + W   GQ KI + C ++  M++L   A S+G+   V+ DAGRTQ++AGS TVL
Sbjct:   93 EVRMALVIRQDLGMTKGKIAAQCCHAALSCFRHIATNPARASYNPIMTQRWLNAGQAKITLKCPDKFTMDELYAKAISLGVNAAVIHDAGRTQIAAGSATVL 194

[Close]

BLAST of FG529936 vs. SwissProt
Match: PTH_PYRHO (Peptidyl-tRNA hydrolase OS=Pyrococcus horikoshii GN=pth PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.281e-14
Identity = 40/95 (42.11%), Postives = 58/95 (61.05%), Query Frame = 2

Query:  200 KMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            K V+V R DLK+  GK+A+Q AH A     E  +  R     W + GQ K+VV  ++++E+ KLK  AE +GLP  ++ DAG T++  G+ TVLA
Sbjct:    8 KQVIVARADLKLSKGKLAAQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKVESEEELFKLKAEAEKLGLPNALIRDAGLTEIPPGTVTVLA 102

[Close]

BLAST of FG529936 vs. TAIR peptide
Match: AT4G32900.2 (| Symbols: | Peptidyl-tRNA hydrolase II (PTH2) family protein | chr4:15878944-15880979 REVERSE LENGTH=193)

HSP 1 Score: 209.149 bits (531), Expect = 9.434e-55
Identity = 111/161 (68.94%), Postives = 132/161 (81.99%), Query Frame = 2

Query:    5 QQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNA-DQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            +Q+ + +S SF+ EN IPG+VIGFI G ++DLS+   + + +    S+ K+Q Q SV  N  DQELKMVLVVRQDLKMR+GKIASQCAHAATGMYAEL QS+R LLR WE+ GQPKIVVTCKNQQEMNK+ E AESVGLPTFVVADAGRT+V+AGS+TVLA
Sbjct:   15 RQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLLSS-KVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQQEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLA 174

[Close]

BLAST of FG529936 vs. TAIR peptide
Match: AT4G32900.1 (| Symbols: | Peptidyl-tRNA hydrolase II (PTH2) family protein | chr4:15879542-15880979 REVERSE LENGTH=140)

HSP 1 Score: 155.221 bits (391), Expect = 1.617e-38
Identity = 83/127 (65.35%), Postives = 100/127 (78.74%), Query Frame = 2

Query:    5 QQKGEWLSGSFKPENFIPGLVIGFICGFLIDLSKPTRNHLSKKIFSSNNKLQHQLSVSSNA-DQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEM 382
            +Q+ + +S SF+ EN IPG+VIGFI G ++DLS+   + + +    S+ K+Q Q SV  N  DQELKMVLVVRQDLKMR+GKIASQCAHAATGMYAEL QS+R LLR WE+ GQPKIVVTCKNQQEM
Sbjct:   15 RQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLLSS-KVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQQEM 140

[Close]

BLAST of FG529936 vs. TAIR peptide
Match: AT5G16870.1 (| Symbols: | Peptidyl-tRNA hydrolase II (PTH2) family protein | chr5:5546975-5548542 REVERSE LENGTH=169)

HSP 1 Score: 115.931 bits (289), Expect = 1.087e-26
Identity = 60/147 (40.82%), Postives = 93/147 (63.27%), Query Frame = 2

Query:   50 FIPGLVIGFICGFLIDL--SKPTRNHLSKKIFSSNNKLQHQLSVSSNADQELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            ++  L++G   G+ I    SKP    + K I + ++K   ++   ++   + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L +     L  WE+C QPK+VV  +++ EM +L+E A+S+ LPT +  DAGRTQ++  S+TV+A
Sbjct:    5 WLSALIVGAALGYCIGTRRSKPVVAAVDKDIKTQSSKSPLEIEKLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDEDEMLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMA 151

[Close]

BLAST of FG529936 vs. TAIR peptide
Match: AT3G03010.2 (| Symbols: | Peptidyl-tRNA hydrolase II (PTH2) family protein | chr3:678982-680137 REVERSE LENGTH=179)

HSP 1 Score: 110.923 bits (276), Expect = 3.497e-25
Identity = 65/152 (42.76%), Postives = 91/152 (59.87%), Query Frame = 2

Query:   62 LVIGFICGFLIDLSKPTRN-HLSKKIF-----SSNNKL---QHQLSVSSNAD--QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            L++G   G+ I   + TR   LS K       SS NK    +  L +   AD  +  KMVLVVR DLKM  GKIA+QC+HA  G+Y +L Q     L  WE C QPK+VV  ++++EM  L+E A+++ LPT +  DAG+TQ++  S+TV+A
Sbjct:   10 LLVGAALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMA 161

[Close]

BLAST of FG529936 vs. TAIR peptide
Match: AT3G03010.1 (| Symbols: | Peptidyl-tRNA hydrolase II (PTH2) family protein | chr3:678982-680137 REVERSE LENGTH=179)

HSP 1 Score: 110.923 bits (276), Expect = 3.497e-25
Identity = 65/152 (42.76%), Postives = 91/152 (59.87%), Query Frame = 2

Query:   62 LVIGFICGFLIDLSKPTRN-HLSKKIF-----SSNNKL---QHQLSVSSNAD--QELKMVLVVRQDLKMRSGKIASQCAHAATGMYAELTQSNRSLLRHWEQCGQPKIVVTCKNQQEMNKLKETAESVGLPTFVVADAGRTQVSAGSKTVLA 484
            L++G   G+ I   + TR   LS K       SS NK    +  L +   AD  +  KMVLVVR DLKM  GKIA+QC+HA  G+Y +L Q     L  WE C QPK+VV  ++++EM  L+E A+++ LPT +  DAG+TQ++  S+TV+A
Sbjct:   10 LLVGAALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMA 161

The following BLAST results are available for this feature:

BLAST of FG529936 vs. TrEMBL
BLAST of FG529936 vs. SwissProt
BLAST of FG529936 vs. TAIR peptide

BLAST of FG529936 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T5M3_SOYBN	2.067e-70	84.47	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7SU93_VITVI	9.622e-60	73.29	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RRZ0_RICCO	1.101e-55	70.81	Peptidyl-tRNA hydrolase, putative OS=Ricinus commu...	[more]
A9PDF5_POPTR	5.464e-55	69.57	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q3E6Z5_ARATH	1.139e-52	68.94	Putative uncharacterized protein At4g32900.2 OS=Ar...	[more]
Q6F2Z0_ORYSJ	8.449e-48	62.13	Os05g0520200 protein OS=Oryza sativa subsp. japoni...	[more]
A2Y6L9_ORYSI	8.449e-48	62.13	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5Z1B8_SORBI	1.441e-47	62.50	Putative uncharacterized protein Sb09g025940 OS=So...	[more]
A9P0R8_PICSI	9.342e-47	60.00	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9TAP2_PHYPA	1.000e-40	53.37	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of FG529936 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PTH2_HUMAN	3.364e-23	39.44	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo s...	[more]
PTH2_BOVIN	3.719e-22	38.73	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos ta...	[more]
PTH2_MOUSE	3.148e-21	37.24	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus mu...	[more]
PTH2_DROME	4.546e-20	50.51	Probable peptidyl-tRNA hydrolase 2 OS=Drosophila m...	[more]
PTH2_SCHPO	5.938e-20	36.05	Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccha...	[more]
PTH_SULTO	1.159e-15	45.92	Peptidyl-tRNA hydrolase OS=Sulfolobus tokodaii GN=...	[more]
PTH_PYRFU	1.159e-15	44.21	Peptidyl-tRNA hydrolase OS=Pyrococcus furiosus GN=...	[more]
PTH2_DICDI	1.513e-15	39.18	Probable peptidyl-tRNA hydrolase 2 OS=Dictyosteliu...	[more]
PTH2_YEAST	1.977e-15	42.16	Peptidyl-tRNA hydrolase 2 OS=Saccharomyces cerevis...	[more]
PTH_PYRHO	1.281e-14	42.11	Peptidyl-tRNA hydrolase OS=Pyrococcus horikoshii G...	[more]

BLAST of FG529936 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G32900.2	9.434e-55	68.94	\| Symbols: \| Peptidyl-tRNA hydrolase II (PTH2) fa...	[more]
AT4G32900.1	1.617e-38	65.35	\| Symbols: \| Peptidyl-tRNA hydrolase II (PTH2) fa...	[more]
AT5G16870.1	1.087e-26	40.82	\| Symbols: \| Peptidyl-tRNA hydrolase II (PTH2) fa...	[more]
AT3G03010.2	3.497e-25	42.76	\| Symbols: \| Peptidyl-tRNA hydrolase II (PTH2) fa...	[more]
AT3G03010.1	3.497e-25	42.76	\| Symbols: \| Peptidyl-tRNA hydrolase II (PTH2) fa...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 65..161 score: 9.6
IPR002833	Peptidyl-tRNA hydrolase, PTH2	TIGRFAMs	TIGR00283	arch_pth2: peptidyl-tRNA hydrolase	coord: 66..161 score: 1.2
None	No IPR available	GENE3D	3.40.1490.10	no description	coord: 65..161 score: 4.1
None	No IPR available	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 7..161 score: 1.4
None	No IPR available	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 64..161 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529936 ID=FG529936; Name=FG529936; organism=Pisum sativum; type=EST; length=486bp
ACGACAGCAAAAAGGAGAATGGTTATCAGGAAGTTTCAAACCAGAGAATT
TCATTCCAGGTCTTGTTATAGGTTTTATTTGTGGCTTCTTGATTGATTTA
TCAAAGCCAACTAGAAATCACTTATCCAAGAAAATTTTCTCATCTAACAA
CAAGCTTCAGCACCAACTCTCAGTCTCAAGTAATGCTGATCAAGAACTTA
AGATGGTTCTGGTTGTTAGGCAAGACCTAAAGATGAGATCCGGAAAGATT
GCGTCTCAATGTGCTCATGCTGCGACTGGCATGTATGCTGAGTTAACGCA
AAGCAACCGGTCTCTTTTAAGACATTGGGAACAGTGCGGCCAGCCCAAAA
TTGTTGTGACTTGCAAGAACCAACAAGAAATGAATAAGCTAAAGGAAACA
GCTGAAAGTGTTGGCCTCCCTACTTTTGTTGTCGCCGATGCTGGACGAAC
ACAGGTTTCGGCAGGATCAAAGACTGTTCTTGCTGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004045	aminoacyl-tRNA hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR002833	PTH2

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