FG530325

EST Overview


Name	FG530325
Unique Name	FG530325
Type	EST
Organism	Pisum sativum (pea)
Sequence length	621

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG530325 vs. TrEMBL
Match: C1L3B4_PEA (Plastidic 2-oxoglutarate/malate translocator (Fragment) OS=Pisum sativum GN=omt PE=2 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 9.148e-85
Identity = 155/168 (92.26%), Postives = 155/168 (92.26%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ITGVVT KECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVN            KAIGLW
Sbjct:  313 ITGVVTCKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNIIIWLGLGSIWWKAIGLW 480

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BLAST of FG530325 vs. TrEMBL
Match: B9GF08_POPTR (2-oxoglutarate/malate translocator OS=Populus trichocarpa GN=POPTRDRAFT_706278 PE=4 SV=1)

HSP 1 Score: 312.383 bits (799), Expect = 1.725e-83
Identity = 148/168 (88.10%), Postives = 155/168 (92.26%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ITGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPP+FGAMVL+FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+IS+VN            KAIGLW
Sbjct:  299 ITGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPYFGAMVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISVVNIIIWLGVGGVWWKAIGLW 466

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BLAST of FG530325 vs. TrEMBL
Match: B9RID8_RICCO (2-oxoglutarate/malate translocator, chloroplast, putative OS=Ricinus communis GN=RCOM_1578230 PE=4 SV=1)

HSP 1 Score: 308.916 bits (790), Expect = 1.907e-82
Identity = 147/168 (87.50%), Postives = 154/168 (91.67%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ITGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPP+FGAMVL+FLSNLMGGLTHYGIGSAPVF+GANYVPLA+WWGYGF+IS+VN            K IGLW
Sbjct:  394 ITGVVTWKECLAEAVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPYFGAMVLAFLSNLMGGLTHYGIGSAPVFYGANYVPLAQWWGYGFLISVVNIIIWLGIGGVWWKFIGLW 561

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BLAST of FG530325 vs. TrEMBL
Match: B9HNW4_POPTR (2-oxoglutarate/malate translocator OS=Populus trichocarpa GN=POPTRDRAFT_821445 PE=4 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 5.550e-82
Identity = 146/168 (86.90%), Postives = 152/168 (90.48%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPP FGAMVL+F SNLMGGLTHYGIGSAPVF+GANYVPLAKWWGYGF+IS+VN            K IGLW
Sbjct:  299 VTGVVTWKECLAEAVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPLFGAMVLAFFSNLMGGLTHYGIGSAPVFYGANYVPLAKWWGYGFLISVVNIIIWLGVGGVWWKFIGLW 466

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BLAST of FG530325 vs. TrEMBL
Match: D7SL49_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025268001 PE=4 SV=1)

HSP 1 Score: 305.834 bits (782), Expect = 1.615e-81
Identity = 145/168 (86.31%), Postives = 153/168 (91.07%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSF IL+LLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPP+FGAMVL+FLSNLMGGLTHYGIGSAP+F+GANYV LAKWWGYGF+ISIVN            KAIGLW
Sbjct:   45 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFSILLLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPYFGAMVLAFLSNLMGGLTHYGIGSAPIFYGANYVSLAKWWGYGFLISIVNIVIWLGVGGVWWKAIGLW 212

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BLAST of FG530325 vs. TrEMBL
Match: A9NVU0_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 3.597e-81
Identity = 145/167 (86.83%), Postives = 151/167 (90.42%), Query Frame = 1

Query:    4 TGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVK VGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPP FGAMVLSFLSNLMGGLTHYGIGSAPVF+GANYVPLA+WWGYGF+IS+VN            K +GLW
Sbjct:  428 TGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKVVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPLFGAMVLSFLSNLMGGLTHYGIGSAPVFYGANYVPLAQWWGYGFLISVVNIVIWLGLGGVWWKVLGLW 594

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BLAST of FG530325 vs. TrEMBL
Match: Q9LXV3_ARATH (2-oxoglutarate/malate translocator-like protein OS=Arabidopsis thaliana GN=T24H18_30 PE=4 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 4.698e-81
Identity = 143/168 (85.12%), Postives = 152/168 (90.48%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI WFSQTVVKFVGGLGLSWQLSFGILVLLYFY+HYFFASGAAHIGAMFTAFLSV+TALGTPP+F A+VL+FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+ISIVN            K IGLW
Sbjct:  390 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGTPPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVGGAWWKFIGLW 557

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BLAST of FG530325 vs. TrEMBL
Match: Q59IV4_MESCR (Putative plastidic 2-oxoglutarate/malate translocator (Fragment) OS=Mesembryanthemum crystallinum GN=DCT1 PE=2 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 4.698e-81
Identity = 144/168 (85.71%), Postives = 153/168 (91.07%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ITGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI+WFS+TVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALG PP+F A+VL+FLSNLMGGLTHYGIGSAPVF+GANYVPLA+WWGYGFVIS+VN            KAIGLW
Sbjct:  259 ITGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIAWFSETVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGAPPYFAAVVLAFLSNLMGGLTHYGIGSAPVFYGANYVPLAQWWGYGFVISVVNILIWLGVGGFWWKAIGLW 426

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BLAST of FG530325 vs. TrEMBL
Match: D7M536_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_909232 PE=4 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 4.698e-81
Identity = 143/168 (85.12%), Postives = 152/168 (90.48%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI WFSQTVVKFVGGLGLSWQLSFGILVLLYFY+HYFFASGAAHIGAMFTAFLSV+TALGTPP+F A+VL+FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+ISIVN            K IGLW
Sbjct:  387 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGTPPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVGGAWWKFIGLW 554

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BLAST of FG530325 vs. TrEMBL
Match: Q93Y83_ARATH (2-oxoglutarate/malate translocator-like protein OS=Arabidopsis thaliana GN=T24H18.30 PE=2 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 3.045e-80
Identity = 142/168 (84.52%), Postives = 151/168 (89.88%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI WFSQTVVKFVGGLGLSWQLSFGILVLLYFY+HYFFASGAAHIGAMFTAFLSV+TALGTPP+F A+V +FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+ISIVN            K IGLW
Sbjct:  246 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGTPPYFAALVHAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVGGAWWKFIGLW 413

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BLAST of FG530325 vs. SwissProt
Match: SOT1_SPIOL (2-oxoglutarate/malate translocator, chloroplastic OS=Spinacia oleracea GN=SODIT1 PE=1 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 3.094e-80
Identity = 141/168 (83.93%), Postives = 150/168 (89.29%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ITGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI+WFS+ VVK VGGLGLSWQ+SFG+LVLLYFYSHYFFASGAAHIGAMFTAFLSVA+ALGTPPF  A+VLSFLSNLMGGLTHYGIGSAPVF+GANYVPL +WWGYGF+ISIVN            KAIGLW
Sbjct:  402 ITGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLITWFSENVVKVVGGLGLSWQMSFGVLVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPFLAAIVLSFLSNLMGGLTHYGIGSAPVFYGANYVPLPQWWGYGFLISIVNLIIWLGVGGLWWKAIGLW 569

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BLAST of FG530325 vs. SwissProt
Match: YFLS_BACSU (Putative malate transporter yflS OS=Bacillus subtilis GN=yflS PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.550e-47
Identity = 84/168 (50.00%), Postives = 114/168 (67.86%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            ++ V+TW +   E  AWDTLTWFAAL+ +A +LN+ G++SWFS  +   V G   SW ++F IL+++Y+YSHYFFAS  AHI AM++AFL+V  A G PP   A+ L+F+SNL G  THYG G+APVFFGA Y+P  KWW  GF++SIV+            K +G+W
Sbjct:  313 LSQVLTWDDIKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKSSVSGF--SWIVAFIILIVVYYYSHYFFASATAHISAMYSAFLAVVVAAGAPPLLAALSLAFISNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW 478

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BLAST of FG530325 vs. SwissProt
Match: Y020_HAEIN (Uncharacterized transporter HI_0020 OS=Haemophilus influenzae GN=HI_0020 PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.615e-20
Identity = 50/150 (33.33%), Postives = 77/150 (51.33%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQT---VVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVIS 441
            +  +VTW + L    AW+TL W+  +I M+G L K G   W + T   +++F G       ++  +++ L     Y FASG A++ AM   F +V    G P    A+ L F ++  G +THYG G  P+ FGA Y  +  WW  G +I+
Sbjct:  310 VLSIVTWDDILKSKGAWNTLVWYGGIIGMSGLLEKSGFFKWLANTLSTILQFEG----HGMMALIVILTLSVSVRYLFASGGAYVAAMVPVFATVGHVTGAPTELLALGLVFANSYGGSVTHYGGGPGPIAFGAGYNDIKSWWITGAIIA 455

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BLAST of FG530325 vs. SwissProt
Match: YBHI_ECOLI (Inner membrane protein ybhI OS=Escherichia coli (strain K12) GN=ybhI PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 4.699e-20
Identity = 47/149 (31.54%), Postives = 78/149 (52.35%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            + G+VTW++ +     W+TL W+  +I ++  L+K     W ++     +   G    ++F +++ L     YFFASG+A+I AM   F  +A   G P    A+ L F ++  G +THYG  + PV FG  Y  +  WW  G V++I+
Sbjct:  308 LLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKNNLAFDGHG-NVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVLTIL 455

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BLAST of FG530325 vs. SwissProt
Match: CITT_ECOLI (Citrate carrier OS=Escherichia coli (strain K12) GN=citT PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.083e-16
Identity = 48/147 (32.65%), Postives = 73/147 (49.66%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLS-NLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            VV WK+      AW+TL   A L+ MA  L + G I WF+ T+   +   G S   +  +LVL+++++HY FAS +AH   M    L+V   +   P     +L  LS  +MG LT Y  G   + +G  YV    +W  G +  ++
Sbjct:  326 VVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLE--GFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVI 470

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BLAST of FG530325 vs. SwissProt
Match: CITT_ECO57 (Citrate carrier OS=Escherichia coli O157:H7 GN=citT PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.083e-16
Identity = 48/147 (32.65%), Postives = 73/147 (49.66%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLS-NLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            VV WK+      AW+TL   A L+ MA  L + G I WF+ T+   +   G S   +  +LVL+++++HY FAS +AH   M    L+V   +   P     +L  LS  +MG LT Y  G   + +G  YV    +W  G +  ++
Sbjct:  326 VVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLE--GFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVI 470

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BLAST of FG530325 vs. SwissProt
Match: TTDT_SHISS (L-tartrate/succinate antiporter OS=Shigella sonnei (strain Ss046) GN=ttdT PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.171e-16
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTP----PFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            +++W + ++   AW+   W A+LI +A  LN  G ISWF + +   +   G S  +    L+++++   YFFAS  A+  A+  A + +A AL  P    P F  MV + +  L   LT Y  G +P+++G+ Y+P A +W  G +  ++
Sbjct:  325 IISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLS--GYSPTMVMVALIVVFYLLRYFFASATAYTSAL--APMMIAAALAMPEIPLPVFCLMVGAAI-GLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLI 469

[Close]

BLAST of FG530325 vs. SwissProt
Match: TTDT_SHIDS (L-tartrate/succinate antiporter OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=ttdT PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.171e-16
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTP----PFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            +++W + ++   AW+   W A+LI +A  LN  G ISWF + +   +   G S  +    L+++++   YFFAS  A+  A+  A + +A AL  P    P F  MV + +  L   LT Y  G +P+++G+ Y+P A +W  G +  ++
Sbjct:  325 IISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLS--GYSPTMVMVALIVVFYLLRYFFASATAYTSAL--APMMIAAALAMPEIPLPVFCLMVGAAI-GLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLI 469

[Close]

BLAST of FG530325 vs. SwissProt
Match: TTDT_SHIBS (L-tartrate/succinate antiporter OS=Shigella boydii serotype 4 (strain Sb227) GN=ttdT PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.171e-16
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTP----PFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            +++W + ++   AW+   W A+LI +A  LN  G ISWF + +   +   G S  +    L+++++   YFFAS  A+  A+  A + +A AL  P    P F  MV + +  L   LT Y  G +P+++G+ Y+P A +W  G +  ++
Sbjct:  325 IISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLS--GYSPTMVMVALIVVFYLLRYFFASATAYTSAL--APMMIAAALAMPEIPLPVFCLMVGAAI-GLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLI 469

[Close]

BLAST of FG530325 vs. SwissProt
Match: TTDT_ECOLI (L-tartrate/succinate antiporter OS=Escherichia coli (strain K12) GN=ttdT PE=1 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 9.171e-16
Identity = 46/150 (30.67%), Postives = 80/150 (53.33%), Query Frame = 1

Query:   10 VVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTP----PFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIV 447
            +++W + ++   AW+   W A+LI +A  LN  G ISWF + +   +   G S  +    L+++++   YFFAS  A+  A+  A + +A AL  P    P F  MV + +  L   LT Y  G +P+++G+ Y+P A +W  G +  ++
Sbjct:  325 IISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLS--GYSPTMVMVALIVVFYLLRYFFASATAYTSAL--APMMIAAALAMPEIPLPVFCLMVGAAI-GLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLI 469

[Close]

BLAST of FG530325 vs. TAIR peptide
Match: AT5G12860.1 (| Symbols: DiT1 | dicarboxylate transporter 1 | chr5:4059927-4061919 REVERSE LENGTH=557)

HSP 1 Score: 304.294 bits (778), Expect = 3.423e-83
Identity = 143/168 (85.12%), Postives = 152/168 (90.48%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI WFSQTVVKFVGGLGLSWQLSFGILVLLYFY+HYFFASGAAHIGAMFTAFLSV+TALGTPP+F A+VL+FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+ISIVN            K IGLW
Sbjct:  390 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGTPPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVGGAWWKFIGLW 557

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BLAST of FG530325 vs. TAIR peptide
Match: AT5G12860.2 (| Symbols: DiT1 | dicarboxylate transporter 1 | chr5:4059850-4061919 REVERSE LENGTH=556)

HSP 1 Score: 300.056 bits (767), Expect = 6.455e-82
Identity = 142/167 (85.03%), Postives = 151/167 (90.42%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGL 501
            +TGVVTWKECLAE VAWDTLTWFAALIAMAGYLNKYGLI WFSQTVVKFVGGLGLSWQLSFGILVLLYFY+HYFFASGAAHIGAMFTAFLSV+TALGTPP+F A+VL+FLSNLMGGLTHYGIGSAP+F+GANYVPLAKWWGYGF+ISIVN            K IGL
Sbjct:  390 VTGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLIEWFSQTVVKFVGGLGLSWQLSFGILVLLYFYTHYFFASGAAHIGAMFTAFLSVSTALGTPPYFAALVLAFLSNLMGGLTHYGIGSAPIFYGANYVPLAKWWGYGFLISIVNILIWLGVGGAWWKFIGL 556

[Close]

BLAST of FG530325 vs. TAIR peptide
Match: AT5G64290.1 (| Symbols: DCT, DIT2.1 | dicarboxylate transport 2.1 | chr5:25714495-25716642 REVERSE LENGTH=563)

HSP 1 Score: 157.918 bits (398), Expect = 3.962e-39
Identity = 72/168 (42.86%), Postives = 101/168 (60.12%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            + GV+ W +CL+E  AWDTL WFA L+ MAG L   G+++W S  V K +  L LSW  +FG+L   YF+ HY FAS   H+GA+F+AFL++  A G P    A+ L++ +NL G LTHY  G A V++GA YV L   +  GFV++ +N            K +GL+
Sbjct:  396 VLGVLNWDDCLSEKSAWDTLAWFAVLVGMAGQLTNLGVVTWMSDCVAKVLQSLSLSWPAAFGLLQAAYFFIHYLFASQTGHVGALFSAFLAMHIAAGVPGILAALALAYNTNLFGALTHYSSGQAAVYYGAGYVDLPDVFKIGFVMATINAIIWGVVGTFWWKFLGLY 563

[Close]

BLAST of FG530325 vs. TAIR peptide
Match: AT5G64280.1 (| Symbols: DiT2.2 | dicarboxylate transporter 2.2 | chr5:25711330-25713411 REVERSE LENGTH=549)

HSP 1 Score: 142.124 bits (357), Expect = 2.250e-34
Identity = 68/168 (40.48%), Postives = 95/168 (56.55%), Query Frame = 1

Query:    1 ITGVVTWKECLAEGVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLSWQLSFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVATALGTPPFFGAMVLSFLSNLMGGLTHYGIGSAPVFFGANYVPLAKWWGYGFVISIVNXXXXXXXXXXXXKAIGLW 504
            + GV+ W +CL++  AWD+LTWFA LI MAG L   G+++W S  V K +  L L+W  SF IL   Y   HY FAS   H GA++  FL++  A G P    A+ L+F +NL G L HY  G A +++GA YV L   +  GFV+++V             K +GL+
Sbjct:  382 LLGVINWDDCLSDKSAWDSLTWFAVLIGMAGQLTNLGVVAWMSDCVAKLLQSLSLTWPASFIILQACYLLIHYLFASQTGHAGALYPPFLAMQIAAGVPGVLAALCLAFNNNLSGALAHYSGGPAALYYGAGYVDLRDMFRVGFVMALVQAIIWGGVGSFWWKFLGLY 549

The following BLAST results are available for this feature:

BLAST of FG530325 vs. TrEMBL
BLAST of FG530325 vs. SwissProt
BLAST of FG530325 vs. TAIR peptide

BLAST of FG530325 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C1L3B4_PEA	9.148e-85	92.26	Plastidic 2-oxoglutarate/malate translocator (Frag...	[more]
B9GF08_POPTR	1.725e-83	88.10	2-oxoglutarate/malate translocator OS=Populus tric...	[more]
B9RID8_RICCO	1.907e-82	87.50	2-oxoglutarate/malate translocator, chloroplast, p...	[more]
B9HNW4_POPTR	5.550e-82	86.90	2-oxoglutarate/malate translocator OS=Populus tric...	[more]
D7SL49_VITVI	1.615e-81	86.31	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9NVU0_PICSI	3.597e-81	86.83	Putative uncharacterized protein OS=Picea sitchens...	[more]
Q9LXV3_ARATH	4.698e-81	85.12	2-oxoglutarate/malate translocator-like protein OS...	[more]
Q59IV4_MESCR	4.698e-81	85.71	Putative plastidic 2-oxoglutarate/malate transloca...	[more]
D7M536_ARALY	4.698e-81	85.12	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q93Y83_ARATH	3.045e-80	84.52	2-oxoglutarate/malate translocator-like protein OS...	[more]

BLAST of FG530325 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SOT1_SPIOL	3.094e-80	83.93	2-oxoglutarate/malate translocator, chloroplastic ...	[more]
YFLS_BACSU	1.550e-47	50.00	Putative malate transporter yflS OS=Bacillus subti...	[more]
Y020_HAEIN	1.615e-20	33.33	Uncharacterized transporter HI_0020 OS=Haemophilus...	[more]
YBHI_ECOLI	4.699e-20	31.54	Inner membrane protein ybhI OS=Escherichia coli (s...	[more]
CITT_ECOLI	1.083e-16	32.65	Citrate carrier OS=Escherichia coli (strain K12) G...	[more]
CITT_ECO57	1.083e-16	32.65	Citrate carrier OS=Escherichia coli O157:H7 GN=cit...	[more]
TTDT_SHISS	9.171e-16	30.67	L-tartrate/succinate antiporter OS=Shigella sonnei...	[more]
TTDT_SHIDS	9.171e-16	30.67	L-tartrate/succinate antiporter OS=Shigella dysent...	[more]
TTDT_SHIBS	9.171e-16	30.67	L-tartrate/succinate antiporter OS=Shigella boydii...	[more]
TTDT_ECOLI	9.171e-16	30.67	L-tartrate/succinate antiporter OS=Escherichia col...	[more]

BLAST of FG530325 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G12860.1	3.423e-83	85.12	\| Symbols: DiT1 \| dicarboxylate transporter 1 \| ch...	[more]
AT5G12860.2	6.455e-82	85.03	\| Symbols: DiT1 \| dicarboxylate transporter 1 \| ch...	[more]
AT5G64290.1	3.962e-39	42.86	\| Symbols: DCT, DIT2.1 \| dicarboxylate transport 2...	[more]
AT5G64280.1	2.250e-34	40.48	\| Symbols: DiT2.2 \| dicarboxylate transporter 2.2 ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001898	Sodium/sulphate symporter	PFAM	PF00939	Na_sulph_symp	coord: 1..168 score: 2.8
None	No IPR available	PANTHER	PTHR10283	CATION TRANSPORTER RELATED	coord: 3..168 score: 2.9
None	No IPR available	PANTHER	PTHR10283:SF13	SODIUM/CARBOXYLATE COTRANSPORTER RELATED	coord: 3..168 score: 2.9
None	No IPR available	SEG	seg	seg	coord: 151..162 score
None	No IPR available	SEG	seg	seg	coord: 33..52 score: NAcoord: 95..105 score
None	No IPR available	SEG	seg	seg	coord: 56..62 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG530325 ID=FG530325; Name=FG530325; organism=Pisum sativum; type=EST; length=621bp
ATCACAGGGGTTGTAACATGGAAGGAGTGCTTAGCTGAGGGTGTTGCCTG
GGATACTCTCACGTGGTTTGCTGCCCTCATTGCAATGGCCGGGTATTTGA
ACAAATACGGTCTTATCTCTTGGTTCAGTCAAACTGTAGTCAAGTTTGTT
GGTGGATTGGGCCTTTCATGGCAACTATCTTTTGGTATTTTGGTTCTCCT
ATACTTCTACTCCCATTACTTCTTTGCCAGTGGAGCTGCTCATATTGGCG
CCATGTTTACTGCATTTTTGTCTGTTGCTACTGCTCTGGGGACTCCACCA
TTCTTCGGAGCCATGGTGCTGTCTTTCCTCTCCAACCTTATGGGCGGCCT
TACTCATTATGGGATTGGGTCAGCTCCAGTGTTTTTCGGTGCCAACTATG
TCCCGCTTGCCAAATGGTGGGGTTACGGCTTTGTTATTAGTATTGTTAAC
ATTATAATCTGGCTTGGACTTGGGTCTATTTGGTGGAAAGCCATTGGCTT
GTGGTAAGGGGTCTGACATAGAATTTTGCCAAATACAGTTTAAGATTTTT
GCATACATCCATGTACGCATTCTTGTTGTTATCAGCAGGTGAAGTAAATT
GCGATTTTTTAACCTATCCTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006814	sodium ion transport
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR001898	SLC13A/DASS

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