FG531458

EST Overview


Name	FG531458
Unique Name	FG531458
Type	EST
Organism	Pisum sativum (pea)
Sequence length	400

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG531458 vs. TrEMBL
Match: B7FI73_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 7.156e-71
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALD+AKGL FLHSDEV+VIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV
Sbjct:  139 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDSAKGLTFLHSDEVEVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 271

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BLAST of FG531458 vs. TrEMBL
Match: C6ZRX8_SOYBN (Protein kinase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 4.493e-65
Identity = 119/133 (89.47%), Postives = 127/133 (95.49%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGY LEDD+RILVYEFV KGSLDNHLFRR SYFQPLSW IRMK+ALDAAKGLAFLHSDEVDVIYRDFKTSNIL+DSNYNAKLSDFG+AK+GPEG KSHVSTR+MGT GYAAPEY+ATGHLTKKSD+
Sbjct:   95 HPNLVKLIGYSLEDDHRILVYEFVAKGSLDNHLFRRGSYFQPLSWNIRMKVALDAAKGLAFLHSDEVDVIYRDFKTSNILLDSNYNAKLSDFGLAKNGPEGDKSHVSTRVMGTYGYAAPEYIATGHLTKKSDI 227

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BLAST of FG531458 vs. TrEMBL
Match: C6TBQ9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 1.707e-64
Identity = 117/133 (87.97%), Postives = 126/133 (94.74%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDD+R+LVYEF+TKGSLDNHLFRRASYFQPLSW IRMK+ALDAAKGLA+LHSDE  VIYRDFK SNIL+DSNYNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEY+ATGHLTKKSDV
Sbjct:  134 HPNLVKLIGYCLEDDHRLLVYEFLTKGSLDNHLFRRASYFQPLSWNIRMKVALDAAKGLAYLHSDEAKVIYRDFKASNILLDSNYNAKLSDFGLAKDGPAGDKSHVSTRVMGTYGYAAPEYMATGHLTKKSDV 266

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BLAST of FG531458 vs. TrEMBL
Match: D7TLU8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016560001 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 8.768e-61
Identity = 111/133 (83.46%), Postives = 123/133 (92.48%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDD+R+LVYEF+ KGSL++HLFRR SYFQPLSW +RMK+AL AAKGLAFLHS+ V VIYRDFKTSNIL+DSNYNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT +SDV
Sbjct:  138 HPNLVKLIGYCLEDDHRLLVYEFMPKGSLEHHLFRRGSYFQPLSWSLRMKVALGAAKGLAFLHSNSVQVIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTRSDV 270

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BLAST of FG531458 vs. TrEMBL
Match: B9T4H9_RICCO (Protein kinase APK1B, chloroplast, putative OS=Ricinus communis GN=RCOM_0000910 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 8.768e-61
Identity = 111/133 (83.46%), Postives = 123/133 (92.48%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDD+R+LVYEF+ KGSL+NHLFRRASY QPLSW +R++IALDAAKGLAFLHSD+  VIYRDFK SNIL+DSNY AKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEY+ATGHLTKKSDV
Sbjct:  132 HPNLVKLIGYCLEDDHRLLVYEFMPKGSLENHLFRRASYVQPLSWNLRIQIALDAAKGLAFLHSDKAKVIYRDFKASNILLDSNYRAKLSDFGLAKDGPTGSKSHVSTRVMGTYGYAAPEYMATGHLTKKSDV 264

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BLAST of FG531458 vs. TrEMBL
Match: B9HTI3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_805513 PE=4 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.496e-60
Identity = 110/133 (82.71%), Postives = 122/133 (91.73%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDD+R+LVYEF+ KGSL+NHLFRRASYFQPLSW +RMK+ALDAAKGL +LHSD+  VIYRDFK SNIL+DSNY AKLSDFG+AKDGP G KSHVSTRIMGT GYAAPEY+ATGHLT +SDV
Sbjct:  137 HPNLVKLIGYCLEDDHRLLVYEFMPKGSLENHLFRRASYFQPLSWNLRMKVALDAAKGLEYLHSDKAKVIYRDFKASNILLDSNYRAKLSDFGLAKDGPTGSKSHVSTRIMGTYGYAAPEYMATGHLTARSDV 269

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BLAST of FG531458 vs. TrEMBL
Match: Q2PEU0_TRIPR (Putative uncharacterized protein OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.694e-60
Identity = 108/133 (81.20%), Postives = 123/133 (92.48%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HP+LV+LIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+ALDAAKGLAFLHS E  VIYRDFKTSNIL+DS+YNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT KSDV
Sbjct:  137 HPHLVRLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWSLRLKVALDAAKGLAFLHSSETKVIYRDFKTSNILLDSDYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDV 269

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BLAST of FG531458 vs. TrEMBL
Match: B5L8D2_SOLLC (Protein kinase 1b OS=Solanum lycopersicum GN=TPK1b PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.694e-60
Identity = 109/133 (81.95%), Postives = 122/133 (91.73%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HP+LVKLIGYCLED++R+LVYEFV +GSL+NHLFRR SYFQPLSWK+R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DSNY AKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEY+ATGHLT KSDV
Sbjct:  131 HPHLVKLIGYCLEDEHRLLVYEFVPRGSLENHLFRRGSYFQPLSWKLRLKVALGAAKGLAFLHSAETKVIYRDFKTSNILLDSNYTAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYMATGHLTSKSDV 263

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BLAST of FG531458 vs. TrEMBL
Match: B9HMI4_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_720623 PE=4 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.266e-59
Identity = 108/133 (81.20%), Postives = 122/133 (91.73%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLEDD+R+LVYEF+ KGSL+NHLFRRASYFQPLSW +RMK+AL AA+GLA+LHSD+  VIYRDFK SNIL+DS+Y AKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEY+ATGHLT KSDV
Sbjct:  120 HPNLVKLIGYCLEDDHRLLVYEFMPKGSLENHLFRRASYFQPLSWNLRMKVALGAAEGLAYLHSDKAKVIYRDFKASNILLDSSYGAKLSDFGLAKDGPTGSKSHVSTRVMGTHGYAAPEYMATGHLTAKSDV 252

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BLAST of FG531458 vs. TrEMBL
Match: B9SZM1_RICCO (Protein kinase APK1A, chloroplast, putative OS=Ricinus communis GN=RCOM_1257650 PE=4 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.654e-59
Identity = 109/133 (81.95%), Postives = 122/133 (91.73%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DSNYNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT +SDV
Sbjct:   67 HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWNLRLKVALGAAKGLAFLHSAENKVIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTARSDV 199

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BLAST of FG531458 vs. SwissProt
Match: APK1B_ARATH (Protein kinase APK1B, chloroplastic OS=Arabidopsis thaliana GN=APK1B PE=2 SV=2)

HSP 1 Score: 231.106 bits (588), Expect = 1.342e-60
Identity = 109/133 (81.95%), Postives = 121/133 (90.98%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+AL AAKGLAFLH+ E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTRIMGT GYAAPEYLATGHLT KSDV
Sbjct:  132 HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDV 264

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BLAST of FG531458 vs. SwissProt
Match: APK1A_ARATH (Protein kinase APK1A, chloroplastic OS=Arabidopsis thaliana GN=APK1A PE=2 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 4.316e-59
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            H +LVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR  YFQPLSWK+R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT KSDV
Sbjct:  131 HRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDV 263

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BLAST of FG531458 vs. SwissProt
Match: NAK_ARATH (Probable serine/threonine-protein kinase NAK OS=Arabidopsis thaliana GN=NAK PE=2 SV=2)

HSP 1 Score: 217.238 bits (552), Expect = 2.005e-56
Identity = 99/133 (74.44%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLE+++R+LVYEF+T+GSL+NHLFRR +++QPLSW  R+++AL AA+GLAFLH+ +  VIYRDFK SNIL+DSNYNAKLSDFG+A+DGP G  SHVSTR+MGT GYAAPEYLATGHL+ KSDV
Sbjct:  131 HPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDV 263

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BLAST of FG531458 vs. SwissProt
Match: BIK1_ARATH (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana GN=BIK1 PE=1 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 8.423e-55
Identity = 97/133 (72.93%), Postives = 118/133 (88.72%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ KGSL+NHLFRR +YF+PL W +R+ +ALDAAKGLAFLHSD V VIYRD K SNIL+D++YNAKLSDFG+A+DGP G  S+VSTR+MGT GYAAPEY+++GHL  +SDV
Sbjct:  130 HPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDV 262

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BLAST of FG531458 vs. SwissProt
Match: Y3545_ARATH (Probable receptor-like protein kinase At3g55450 OS=Arabidopsis thaliana GN=At3g55450 PE=1 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 3.913e-52
Identity = 97/134 (72.39%), Postives = 117/134 (87.31%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASY-FQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED+ R+LVYEF+ KGSL+NHLF   +  F+PLSW +R+K+ALDAAKGLAFLHSD V VIYRD K SNIL+DS++NAKLSDFG+A+DGP G +S+VSTR+MGT GYAAPEY++TGHL  +SDV
Sbjct:  124 HPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLFANGNKDFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRVMGTFGYAAPEYVSTGHLNARSDV 257

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BLAST of FG531458 vs. SwissProt
Match: APK2B_ARATH (Protein kinase 2B, chloroplastic OS=Arabidopsis thaliana GN=APK2B PE=1 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 1.817e-49
Identity = 91/133 (68.42%), Postives = 112/133 (84.21%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKL+GYC+E + R+LVYEF+ KGSL+NHLFRR +  QPL+W IRMK+A+ AAKGL FLH  +  VIYRDFK +NIL+D+ +N+KLSDFG+AK GP G K+HVST++MGT GYAAPEY+ATG LT KSDV
Sbjct:  146 HPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGA--QPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDV 276

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BLAST of FG531458 vs. SwissProt
Match: APK2A_ARATH (Protein kinase 2A, chloroplastic OS=Arabidopsis thaliana GN=APK2A PE=1 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 7.636e-48
Identity = 89/133 (66.92%), Postives = 110/133 (82.71%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLV L+GYC E + R+LVYEF+ KGSL+NHLFRR +  QPL+W IRMK+A+ AAKGL FLH  +  VIYRDFK +NIL+D+++NAKLSDFG+AK GP G  +HVST+++GT GYAAPEY+ATG LT KSDV
Sbjct:  149 HPNLVLLVGYCAEGENRLLVYEFMPKGSLENHLFRRGA--QPLTWAIRMKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDV 279

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BLAST of FG531458 vs. SwissProt
Match: CX32_ARATH (Probable serine/threonine-protein kinase Cx32, chloroplastic OS=Arabidopsis thaliana GN=At4g35600 PE=1 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 7.147e-46
Identity = 91/133 (68.42%), Postives = 107/133 (80.45%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            H NLVKL+GYC ED   +LVYEF+ KGSL++HLFRR     P  W +R+KI + AA+GLAFLHS + +VIYRDFK SNIL+DSNY+AKLSDFG+AK GP   KSHV+TRIMGT GYAAPEY+ATGHL  KSDV
Sbjct:  149 HRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRIKIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTRIMGTYGYAAPEYMATGHLYVKSDV 278

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BLAST of FG531458 vs. SwissProt
Match: PBS1_ARATH (Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana GN=PBS1 PE=1 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 9.041e-41
Identity = 84/134 (62.69%), Postives = 97/134 (72.39%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSD-EVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLV LIGYC + D R+LVYEF+  GSL++HL       + L W +RMKIA  AAKGL FLH      VIYRDFK+SNIL+D  ++ KLSDFG+AK GP G KSHVSTR+MGT GY APEY  TG LT KSDV
Sbjct:  140 HPNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVKSDV 273

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BLAST of FG531458 vs. SwissProt
Match: RLCK7_ARATH (Probable serine/threonine-protein kinase RLCKVII OS=Arabidopsis thaliana GN=At1g07870 PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.705e-39
Identity = 80/134 (59.70%), Postives = 97/134 (72.39%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVD-VIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIG+C E D R+LVYE++ +GSL++HL    S  +PL W  RMKIA  AA+GL +LH      VIYRD K SNIL+  +Y  KLSDFG+AK GP G K+HVSTR+MGT GY AP+Y  TG LT KSD+
Sbjct:  157 HPNLVKLIGFCAEGDQRLLVYEYMPQGSLEDHLHVLPSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKCSNILLGEDYQPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDI 290

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BLAST of FG531458 vs. TAIR peptide
Match: AT2G28930.3 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424551-12426565 FORWARD LENGTH=415)

HSP 1 Score: 231.106 bits (588), Expect = 1.492e-61
Identity = 109/133 (81.95%), Postives = 121/133 (90.98%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+AL AAKGLAFLH+ E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTRIMGT GYAAPEYLATGHLT KSDV
Sbjct:  135 HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDV 267

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT2G28930.2 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424551-12426565 FORWARD LENGTH=412)

HSP 1 Score: 231.106 bits (588), Expect = 1.492e-61
Identity = 109/133 (81.95%), Postives = 121/133 (90.98%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+AL AAKGLAFLH+ E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTRIMGT GYAAPEYLATGHLT KSDV
Sbjct:  132 HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDV 264

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT2G28930.1 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424957-12426565 FORWARD LENGTH=423)

HSP 1 Score: 231.106 bits (588), Expect = 1.492e-61
Identity = 109/133 (81.95%), Postives = 121/133 (90.98%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR SYFQPLSW +R+K+AL AAKGLAFLH+ E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTRIMGT GYAAPEYLATGHLT KSDV
Sbjct:  143 HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDV 275

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT1G07570.3 (| Symbols: APK1A | Protein kinase superfamily protein | chr1:2331369-2333589 REVERSE LENGTH=424)

HSP 1 Score: 226.098 bits (575), Expect = 4.801e-60
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            H +LVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR  YFQPLSWK+R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT KSDV
Sbjct:  145 HRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDV 277

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT1G07570.1 (| Symbols: APK1A, APK1 | Protein kinase superfamily protein | chr1:2331369-2333210 REVERSE LENGTH=410)

HSP 1 Score: 226.098 bits (575), Expect = 4.801e-60
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            H +LVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR  YFQPLSWK+R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT KSDV
Sbjct:  131 HRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDV 263

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT1G07570.2 (| Symbols: APK1A, APK1 | Protein kinase superfamily protein | chr1:2331369-2333210 REVERSE LENGTH=410)

HSP 1 Score: 226.098 bits (575), Expect = 4.801e-60
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            H +LVKLIGYCLED++R+LVYEF+ +GSL+NHLFRR  YFQPLSWK+R+K+AL AAKGLAFLHS E  VIYRDFKTSNIL+DS YNAKLSDFG+AKDGP G KSHVSTR+MGT GYAAPEYLATGHLT KSDV
Sbjct:  131 HRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDV 263

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT5G02290.2 (| Symbols: NAK | Protein kinase superfamily protein | chr5:470387-472397 REVERSE LENGTH=389)

HSP 1 Score: 217.238 bits (552), Expect = 2.230e-57
Identity = 99/133 (74.44%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLE+++R+LVYEF+T+GSL+NHLFRR +++QPLSW  R+++AL AA+GLAFLH+ +  VIYRDFK SNIL+DSNYNAKLSDFG+A+DGP G  SHVSTR+MGT GYAAPEYLATGHL+ KSDV
Sbjct:  131 HPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDV 263

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT5G02290.1 (| Symbols: NAK | Protein kinase superfamily protein | chr5:470387-472397 REVERSE LENGTH=389)

HSP 1 Score: 217.238 bits (552), Expect = 2.230e-57
Identity = 99/133 (74.44%), Postives = 120/133 (90.23%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLE+++R+LVYEF+T+GSL+NHLFRR +++QPLSW  R+++AL AA+GLAFLH+ +  VIYRDFK SNIL+DSNYNAKLSDFG+A+DGP G  SHVSTR+MGT GYAAPEYLATGHL+ KSDV
Sbjct:  131 HPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDV 263

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT2G39660.1 (| Symbols: BIK1 | botrytis-induced kinase1 | chr2:16531943-16533601 FORWARD LENGTH=395)

HSP 1 Score: 211.846 bits (538), Expect = 9.369e-56
Identity = 97/133 (72.93%), Postives = 118/133 (88.72%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVDVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKLIGYCLED++R+LVYEF+ KGSL+NHLFRR +YF+PL W +R+ +ALDAAKGLAFLHSD V VIYRD K SNIL+D++YNAKLSDFG+A+DGP G  S+VSTR+MGT GYAAPEY+++GHL  +SDV
Sbjct:  130 HPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDV 262

[Close]

BLAST of FG531458 vs. TAIR peptide
Match: AT5G15080.1 (| Symbols: | Protein kinase superfamily protein | chr5:4886414-4888555 FORWARD LENGTH=493)

HSP 1 Score: 203.371 bits (516), Expect = 3.332e-53
Identity = 98/134 (73.13%), Postives = 116/134 (86.57%), Query Frame = 2

Query:    2 HPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDAAKGLAFLHSDEVD-VIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDV 400
            HPNLVKL+GYC+EDD R+LVYEF+ +GSL+NHLFRR+    PL W IRMKIAL AAKGL+FLH + +  VIYRDFKTSNIL+D++YNAKLSDFG+AKD P+ GK+HVSTR+MGT GYAAPEY+ TGHLT KSDV
Sbjct:  205 HPNLVKLVGYCIEDDQRLLVYEFMPRGSLENHLFRRSL---PLPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDADYNAKLSDFGLAKDAPDEGKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDV 335

The following BLAST results are available for this feature:

BLAST of FG531458 vs. TrEMBL
BLAST of FG531458 vs. SwissProt
BLAST of FG531458 vs. TAIR peptide

BLAST of FG531458 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FI73_MEDTR	7.156e-71	97.74	Putative uncharacterized protein (Fragment) OS=Med...	[more]
C6ZRX8_SOYBN	4.493e-65	89.47	Protein kinase OS=Glycine max PE=2 SV=1	[more]
C6TBQ9_SOYBN	1.707e-64	87.97	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TLU8_VITVI	8.768e-61	83.46	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9T4H9_RICCO	8.768e-61	83.46	Protein kinase APK1B, chloroplast, putative OS=Ric...	[more]
B9HTI3_POPTR	1.496e-60	82.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q2PEU0_TRIPR	9.694e-60	81.20	Putative uncharacterized protein OS=Trifolium prat...	[more]
B5L8D2_SOLLC	9.694e-60	81.95	Protein kinase 1b OS=Solanum lycopersicum GN=TPK1b...	[more]
B9HMI4_POPTR	1.266e-59	81.20	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9SZM1_RICCO	1.654e-59	81.95	Protein kinase APK1A, chloroplast, putative OS=Ric...	[more]

BLAST of FG531458 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
APK1B_ARATH	1.342e-60	81.95	Protein kinase APK1B, chloroplastic OS=Arabidopsis...	[more]
APK1A_ARATH	4.316e-59	80.45	Protein kinase APK1A, chloroplastic OS=Arabidopsis...	[more]
NAK_ARATH	2.005e-56	74.44	Probable serine/threonine-protein kinase NAK OS=Ar...	[more]
BIK1_ARATH	8.423e-55	72.93	Serine/threonine-protein kinase BIK1 OS=Arabidopsi...	[more]
Y3545_ARATH	3.913e-52	72.39	Probable receptor-like protein kinase At3g55450 OS...	[more]
APK2B_ARATH	1.817e-49	68.42	Protein kinase 2B, chloroplastic OS=Arabidopsis th...	[more]
APK2A_ARATH	7.636e-48	66.92	Protein kinase 2A, chloroplastic OS=Arabidopsis th...	[more]
CX32_ARATH	7.147e-46	68.42	Probable serine/threonine-protein kinase Cx32, chl...	[more]
PBS1_ARATH	9.041e-41	62.69	Serine/threonine-protein kinase PBS1 OS=Arabidopsi...	[more]
RLCK7_ARATH	1.705e-39	59.70	Probable serine/threonine-protein kinase RLCKVII O...	[more]

BLAST of FG531458 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G28930.3	1.492e-61	81.95	\| Symbols: APK1B, PK1B \| protein kinase 1B \| chr2:...	[more]
AT2G28930.2	1.492e-61	81.95	\| Symbols: APK1B, PK1B \| protein kinase 1B \| chr2:...	[more]
AT2G28930.1	1.492e-61	81.95	\| Symbols: APK1B, PK1B \| protein kinase 1B \| chr2:...	[more]
AT1G07570.3	4.801e-60	80.45	\| Symbols: APK1A \| Protein kinase superfamily prot...	[more]
AT1G07570.1	4.801e-60	80.45	\| Symbols: APK1A, APK1 \| Protein kinase superfamil...	[more]
AT1G07570.2	4.801e-60	80.45	\| Symbols: APK1A, APK1 \| Protein kinase superfamil...	[more]
AT5G02290.2	2.230e-57	74.44	\| Symbols: NAK \| Protein kinase superfamily protei...	[more]
AT5G02290.1	2.230e-57	74.44	\| Symbols: NAK \| Protein kinase superfamily protei...	[more]
AT2G39660.1	9.369e-56	72.93	\| Symbols: BIK1 \| botrytis-induced kinase1 \| chr2:...	[more]
AT5G15080.1	3.332e-53	73.13	\| Symbols: \| Protein kinase superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..133 score: 26
IPR002290	Serine/threonine-protein kinase domain	SMART	SM00220	no description	coord: 1..133 score: 0.
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 69..81 score
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..133 score: 1.9
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 1..133 score: 5.3
IPR020635	Tyrosine-protein kinase, catalytic domain	SMART	SM00219	no description	coord: 1..133 score: 0.0
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 31..133 score: 1.1
None	No IPR available	GENE3D	3.30.200.20	no description	coord: 1..30 score: 7.6
None	No IPR available	PANTHER	PTHR23258	SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE	coord: 1..133 score: 6.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531458 ID=FG531458; Name=FG531458; organism=Pisum sativum; type=EST; length=400bp
TCATCCTAATCTTGTGAAACTAATTGGTTACTGCTTAGAGGATGATTACC
GGATTTTGGTATATGAATTTGTTACCAAGGGAAGTTTGGATAACCATTTG
TTTAGAAGGGCTTCTTACTTTCAGCCACTCTCTTGGAAAATCCGAATGAA
GATAGCTCTTGATGCTGCTAAAGGTCTTGCGTTTCTTCATAGCGATGAAG
TCGATGTGATATACCGAGACTTCAAAACTTCTAATATCTTGATTGATTCT
AACTATAATGCAAAACTCTCCGATTTTGGGATGGCAAAAGATGGACCAGA
AGGCGGTAAGAGCCACGTATCTACAAGGATAATGGGCACCCCAGGTTATG
CGGCTCCCGAGTATCTAGCCACAGGTCACCTAACCAAAAAGAGTGACGTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding
GO:0004674	protein serine/threonine kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR002290	Ser/Thr_dual-sp_kinase
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain
IPR020635	Tyr_kinase_cat_dom

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