FG531753

EST Overview


Name	FG531753
Unique Name	FG531753
Type	EST
Organism	Pisum sativum (pea)
Sequence length	444

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG531753 vs. TrEMBL
Match: O82460_LOTJA (Rac GTPase activating protein 3 (Fragment) OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 1.352e-61
Identity = 117/147 (79.59%), Postives = 126/147 (85.71%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SM CSYDSKGNSVPTILLLMQERLYSQGGL AEGIFRINPENG+EEHLR+QLN G+VP++IDVHCLAGLIKAWFRELPSGVLDGLSPE+VLECNT                ALLNWA+DLMADVV E ++NKMDARNIAMVFAPNMT
Sbjct:   91 SMHCSYDSKGNSVPTILLLMQERLYSQGGLMAEGIFRINPENGQEEHLRDQLNRGVVPDNIDVHCLAGLIKAWFRELPSGVLDGLSPEQVLECNTEEEFVQLVKQLKPTELALLNWALDLMADVVEEEEHNKMDARNIAMVFAPNMT 237

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BLAST of FG531753 vs. TrEMBL
Match: B9S3Y4_RICCO (Gtpase activating protein, putative OS=Ricinus communis GN=RCOM_0556890 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 8.761e-61
Identity = 116/147 (78.91%), Postives = 126/147 (85.71%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYDSKGNSVPTILLLMQERLYSQGGLK EGIFRINPENG+EEH+R+QLN GIVP++I+VHCLAGLIKAWFRELPSGVLDGLSPE+VL+CNT                ALLNWA+DLMADVV E D NKM+ARNIAMVFAPNMT
Sbjct:   94 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKTEGIFRINPENGQEEHVRDQLNRGIVPDNINVHCLAGLIKAWFRELPSGVLDGLSPEQVLQCNTEEESVELVKQLNPTDSALLNWAVDLMADVVQEEDSNKMNARNIAMVFAPNMT 240

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BLAST of FG531753 vs. TrEMBL
Match: B9I9Y6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_246022 PE=4 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.265e-59
Identity = 114/147 (77.55%), Postives = 125/147 (85.03%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYDSKGNSVPTILLLMQ+RLYSQGGLKAEGIFRINPEN +EEH+R+QLN GIVP+DIDVHCLAGLIKAWFRELPSGVLDGLSPE+VL+CNT                ALL+WA+ LMADVV E D NKM+ARNIAMVF+PNMT
Sbjct:  120 SMQCSYDSKGNSVPTILLLMQDRLYSQGGLKAEGIFRINPENSQEEHVRDQLNRGIVPDDIDVHCLAGLIKAWFRELPSGVLDGLSPEQVLQCNTEEESVELVKQLKPTEAALLSWAVGLMADVVEEEDSNKMNARNIAMVFSPNMT 266

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BLAST of FG531753 vs. TrEMBL
Match: Q9ZNR7_ARATH (Putative rac GTPase activating protein OS=Arabidopsis thaliana GN=AT4g03100 PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.818e-59
Identity = 115/147 (78.23%), Postives = 122/147 (82.99%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYD KGNSVPTILLLMQERLYSQ GLKAEGIFRINPEN +EEH+R+QLN GIVP +IDVHCLAGLIKAWFRELPSGVLDGLSPEEVL CNT                ALLNWA+DLMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:  123 SMQCSYDEKGNSVPTILLLMQERLYSQQGLKAEGIFRINPENSQEEHVRDQLNRGIVPENIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLNCNTEDESVELIKQLKPTESALLNWAVDLMADVVEEEESNKMNARNIAMVFAPNMT 269

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BLAST of FG531753 vs. TrEMBL
Match: D7M3A3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_490253 PE=4 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.071e-58
Identity = 114/147 (77.55%), Postives = 121/147 (82.31%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYD KGNSVPTILLLMQERLYSQ GLKAEGIFRINPEN +EEH+R+QLN GIVP +IDVHCLAGLIKAWFRELP GVLDGLSPEEVL CNT                ALLNWA+DLMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:  125 SMQCSYDEKGNSVPTILLLMQERLYSQEGLKAEGIFRINPENSQEEHVRDQLNRGIVPENIDVHCLAGLIKAWFRELPCGVLDGLSPEEVLNCNTEDESVELIKQLKPTESALLNWAVDLMADVVEEEESNKMNARNIAMVFAPNMT 271

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BLAST of FG531753 vs. TrEMBL
Match: D7TP55_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_104.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011015001 PE=4 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.386e-58
Identity = 113/147 (76.87%), Postives = 124/147 (84.35%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYDSKGNSVPTILLLMQERLYSQ GLKAEGIFRINPEN +EE +R+QLN GIVP DIDVHCLAGLIKAWFRELPSG+LDGLSPE+VL+C+T                ALL+WAIDLMADVV E ++NKM+ARNIAMVFAPNMT
Sbjct:   52 SMQCSYDSKGNSVPTILLLMQERLYSQEGLKAEGIFRINPENSQEEIVRDQLNRGIVPTDIDVHCLAGLIKAWFRELPSGILDGLSPEQVLQCSTEEESVELIKQLRPTEAALLSWAIDLMADVVEEEEFNKMNARNIAMVFAPNMT 198

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BLAST of FG531753 vs. TrEMBL
Match: C5YPA0_SORBI (Putative uncharacterized protein Sb08g016850 OS=Sorghum bicolor GN=Sb08g016850 PE=4 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 1.309e-56
Identity = 107/147 (72.79%), Postives = 121/147 (82.31%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQC+YD KGNSVPTILLLMQERLY+Q GLKAEGIFRINPEN +EEH+R+QLN G+VP DIDVHCLA LIKAWFRELP GVLDGLSPE+VL+CN+                ALLNWA++LM+DVV E + NKM+ARNIAMVFAPNMT
Sbjct:  141 SMQCTYDGKGNSVPTILLLMQERLYAQEGLKAEGIFRINPENDQEEHVRDQLNKGVVPEDIDVHCLASLIKAWFRELPEGVLDGLSPEQVLQCNSEGEFLDLVSMLRPTQAALLNWAVELMSDVVEEEELNKMNARNIAMVFAPNMT 287

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BLAST of FG531753 vs. TrEMBL
Match: Q6UQ73_ORYSJ (Os12g0533400 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os12g0533400 PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.233e-56
Identity = 107/147 (72.79%), Postives = 120/147 (81.63%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQC+YD KGNSVPTILL MQERLY+QGGLKAEGIFRINPEN +EEH+R+QLN G+VP DIDVHCLA LIKAWFRELP GVLD LSPE+VL+CN+                ALLNWA++LMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:   52 SMQCTYDGKGNSVPTILLHMQERLYAQGGLKAEGIFRINPENDQEEHVRDQLNKGVVPEDIDVHCLASLIKAWFRELPEGVLDSLSPEQVLQCNSEGEFLELVTLLRPTQAALLNWAVELMADVVEEEELNKMNARNIAMVFAPNMT 198

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BLAST of FG531753 vs. TrEMBL
Match: Q2QPD6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=LOC_Os12g34840 PE=4 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.233e-56
Identity = 107/147 (72.79%), Postives = 120/147 (81.63%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQC+YD KGNSVPTILL MQERLY+QGGLKAEGIFRINPEN +EEH+R+QLN G+VP DIDVHCLA LIKAWFRELP GVLD LSPE+VL+CN+                ALLNWA++LMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:  138 SMQCTYDGKGNSVPTILLHMQERLYAQGGLKAEGIFRINPENDQEEHVRDQLNKGVVPEDIDVHCLASLIKAWFRELPEGVLDSLSPEQVLQCNSEGEFLELVTLLRPTQAALLNWAVELMADVVEEEELNKMNARNIAMVFAPNMT 284

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BLAST of FG531753 vs. TrEMBL
Match: B8BM94_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_38575 PE=4 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.108e-55
Identity = 106/147 (72.11%), Postives = 119/147 (80.95%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQC+YD KGNSVP ILL MQERLY+QGGLKAEGIFRINPEN +EEH+R+QLN G+VP DIDVHCLA LIKAWFRELP GVLD LSPE+VL+CN+                ALLNWA++LMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:   19 SMQCTYDGKGNSVPIILLHMQERLYAQGGLKAEGIFRINPENDQEEHVRDQLNKGVVPEDIDVHCLASLIKAWFRELPEGVLDSLSPEQVLQCNSEEEFLELVTLLRPTQAALLNWAVELMADVVEEEELNKMNARNIAMVFAPNMT 165

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BLAST of FG531753 vs. SwissProt
Match: GACA_DICDI (Rho GTPase-activating protein gacA OS=Dictyostelium discoideum GN=gacA PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 8.733e-19
Identity = 49/140 (35.00%), Postives = 77/140 (55.00%), Query Frame = 3

Query:   21 DSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            + +G  VP IL LM+  L   GGL+ EGIFR+  E  E + L+E +N     +  D++ +A LIK W+RELP+ +L+ +  E++                     +LL+W + L+  V   +D NKM A+N+A+V APN+
Sbjct:  155 EDEGFRVPYILALMKRSLIDNGGLQQEGIFRLAGEQTEIKRLKEAMNRNDFTSSTDINTVASLIKIWYRELPTPILNSIPTEKIFYSTDIDECVQSAKNLPEPQKSLLDWLMHLLLMVSSFSDVNKMTAQNLAIVVAPNL 294

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BLAST of FG531753 vs. SwissProt
Match: GACO_DICDI (Rho GTPase-activating protein gacO OS=Dictyostelium discoideum GN=gacO PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 5.132e-11
Identity = 44/136 (32.35%), Postives = 64/136 (47.06%), Query Frame = 3

Query:   42 PTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDI-DVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVE--ADYNKMDARNIAMVFAPNM 440
            P IL L+ E + S  G   EGIFRI     E   L++Q+N      D  D H LAGL+K W REL   ++      + ++  +                 +LN+ I  + +V     A Y+KMD  N+AMVF+P +
Sbjct:  520 PLILKLLTESIMSLNGPYTEGIFRITGSGTEVNRLKKQINEHDFSLDTQDPHVLAGLLKLWLRELVHPIIPSELYNDAIKSRSKEEVSRIISLIPDQNKEVLNFLIPFLKNVSQPHYAQYSKMDIDNVAMVFSPGL 655

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BLAST of FG531753 vs. SwissProt
Match: RGA2_SCHPO (Probable Rho-type GTPase-activating protein 2 OS=Schizosaccharomyces pombe GN=rga2 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.070e-8
Identity = 40/149 (26.85%), Postives = 67/149 (44.97%), Query Frame = 3

Query:   33 NSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGI------VPNDIDVHCLAGLIKAWFRELPSGVLD-------GLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            + +P ++    E L S    K EGI+R++      +HL+EQ N G+         + DVH +AGL+K + R LP+ +LD        L P    +                   ALL+  +  +  ++     NKM+ RN+ +VF+P +
Sbjct: 1080 SGLPIVVYRCIEYLESCRAEKEEGIYRLSGSASTIKHLKEQFNEGVDYDLLSSDEEFDVHVIAGLLKLYLRNLPTNLLDTSMHKLFELLPNVPNDSAALGELCDVISKLPPENFALLDSLLHHLRRIIAFEKVNKMNIRNVCIVFSPTL 1228

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BLAST of FG531753 vs. SwissProt
Match: RHG24_RAT (Rho GTPase-activating protein 24 OS=Rattus norvegicus GN=Arhgap24 PE=2 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 3.113e-8
Identity = 42/149 (28.19%), Postives = 66/149 (44.30%), Query Frame = 3

Query:   30 GNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPN---DIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXA---------LLNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            GN +  +L+        Q GLK EG+FR+  +    + L++  + G  P+   + DVH +A L+K + RELP  V+     E+ L C T                          LL +    + +V   +  NKM A+N+A VF PN+
Sbjct:  147 GNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLSCATLLSKEEEAGVKELTKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNI 295

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BLAST of FG531753 vs. SwissProt
Match: RHG24_MOUSE (Rho GTPase-activating protein 24 OS=Mus musculus GN=Arhgap24 PE=2 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 3.113e-8
Identity = 42/149 (28.19%), Postives = 66/149 (44.30%), Query Frame = 3

Query:   30 GNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPN---DIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXA---------LLNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            GN +  +L+        Q GLK EG+FR+  +    + L++  + G  P+   + DVH +A L+K + RELP  V+     E+ L C T                          LL +    + +V   +  NKM A+N+A VF PN+
Sbjct:  146 GNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLSCATLLSKEEEAGVKELMKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNI 294

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BLAST of FG531753 vs. SwissProt
Match: MGP4_DICDI (Mental retardation GTPase activating protein homolog 4 OS=Dictyostelium discoideum GN=mgp4 PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.066e-8
Identity = 38/142 (26.76%), Postives = 69/142 (48.59%), Query Frame = 3

Query:   39 VPTILLLMQ---ERLYSQGGLKAEGIFRINPENGEEEHLREQLNSG----IVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADY--NKMDARNIAMVFAPN 437
            +P IL+L+    ++L +  G+K EGIFRI     E   L++ +N        P+   +H +A L+K W RE+P  ++     ++ LEC +                 ++ +    + ++V   +   +KM+  N+AMVFAP+
Sbjct:  340 IPYILVLLVNLIKKLDNGMGMKTEGIFRIPGHTSEVNALKKLINEQGEYQFPPDLYSIHPIASLLKLWLREMPQPLIPNYVYDKSLECQSIEEFIVFFKFLPASNQKIITYLAGFLNELVQPDNVVTSKMNLDNVAMVFAPS 481

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BLAST of FG531753 vs. SwissProt
Match: RHG23_MOUSE (Rho GTPase-activating protein 23 OS=Mus musculus GN=Arhgap23 PE=1 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 1.545e-7
Identity = 41/159 (25.79%), Postives = 72/159 (45.28%), Query Frame = 3

Query:    9 QCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDI--------DVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXAL-------LNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            +C   ++   VP I+     R+    GL++ GI+R+   N     L+EQLN G  P+DI        D++ ++ L+KA+FR+LP  +       + +E N                  L       L + +  +  +   ++ NKM+ RN+A+VF P +
Sbjct:  905 ECQPATENQRVPLIVAACC-RIVEARGLESTGIYRVPGNNAVVSSLQEQLNRG--PSDINLQDERWQDLNVISSLLKAFFRKLPEPLFTDDKYNDFIEANRIEDSRERMKTLRKLIRDLPGHYYETLKFLVGHLKTIADHSEKNKMEPRNLALVFGPTL 1060

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BLAST of FG531753 vs. SwissProt
Match: RG68F_DROME (Rho GTPase-activating protein 68F OS=Drosophila melanogaster GN=RhoGAP68F PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.545e-7
Identity = 40/144 (27.78%), Postives = 68/144 (47.22%), Query Frame = 3

Query:   33 NSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSG--IVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVL-------ECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPN 437
            NS+P I+    + L   G +  EGIFR +  + E   L+E++N G  +    ++VH +AGL+K++ R+L   +L     E+V        E  +                 L  + ++ +  V+   D NKM + N+A+VF PN
Sbjct:  289 NSIPPIVRKCVDSLSITGVIDTEGIFRRSGNHSEIMALKERVNRGEDVDLKSVNVHVIAGLLKSFLRDLAEPLLTFELYEDVTGFLDWPKEERSRNVTQLIREKLPEENYELFKYIVEFLVRVMDCEDLNKMTSSNLAIVFGPN 432

[Close]

BLAST of FG531753 vs. SwissProt
Match: GACY_DICDI (Rho GTPase-activating protein gacY OS=Dictyostelium discoideum GN=gacY PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.018e-7
Identity = 38/132 (28.79%), Postives = 64/132 (48.48%), Query Frame = 3

Query:   78 SQGGLKAEGIFRINPENGEEEHLREQLNSG--IVPNDI-DVHCLAGLIKAWFRELPSGVLD--------GLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            S+ GL+ EG+FR++  N + + LR+  + G  +   D+ DVH +AGL+K + RELPS +           +S  E  +                   AL       +  V+  +  NKM++ N+++VFAPN+
Sbjct:  555 SRRGLQVEGLFRLSGANSQIKSLRQGFDQGEDVDLEDVEDVHTVAGLLKLYLRELPSPLFPFDLYSSFIEISKGEQTKPQKVESLKLLLSLLPPANKALSKHLFKFLGKVIENSSVNKMNSVNLSIVFAPNL 686

[Close]

BLAST of FG531753 vs. SwissProt
Match: RHG23_HUMAN (Rho GTPase-activating protein 23 OS=Homo sapiens GN=ARHGAP23 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 4.496e-7
Identity = 40/159 (25.16%), Postives = 71/159 (44.65%), Query Frame = 3

Query:    9 QCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDI--------DVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXAL-------LNWAIDLMADVVVEADYNKMDARNIAMVFAPNM 440
            +C   ++   VP I+     R+    GL++ GI+R+   N     L+EQLN G  P DI        D++ ++ L+K++FR+LP  +       + +E N                  L       L + +  +  +   ++ NKM+ RN+A+VF P +
Sbjct:  909 ECQPATENQRVPLIVAACC-RIVEARGLESTGIYRVPGNNAVVSSLQEQLNRG--PGDINLQDERWQDLNVISSLLKSFFRKLPEPLFTDDKYNDFIEANRIEDARERMRTLRKLIRDLPGHYYETLKFLVGHLKTIADHSEKNKMEPRNLALVFGPTL 1064

[Close]

BLAST of FG531753 vs. TAIR peptide
Match: AT4G03100.1 (| Symbols: | Rho GTPase activating protein with PAK-box/P21-Rho-binding domain | chr4:1374361-1375913 FORWARD LENGTH=430)

HSP 1 Score: 231.106 bits (588), Expect = 1.887e-61
Identity = 115/147 (78.23%), Postives = 122/147 (82.99%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYD KGNSVPTILLLMQERLYSQ GLKAEGIFRINPEN +EEH+R+QLN GIVP +IDVHCLAGLIKAWFRELPSGVLDGLSPEEVL CNT                ALLNWA+DLMADVV E + NKM+ARNIAMVFAPNMT
Sbjct:  129 SMQCSYDEKGNSVPTILLLMQERLYSQQGLKAEGIFRINPENSQEEHVRDQLNRGIVPENIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLNCNTEDESVELIKQLKPTESALLNWAVDLMADVVEEEESNKMNARNIAMVFAPNMT 275

[Close]

BLAST of FG531753 vs. TAIR peptide
Match: AT2G46710.1 (| Symbols: | Rho GTPase activating protein with PAK-box/P21-Rho-binding domain | chr2:19192105-19194656 FORWARD LENGTH=455)

HSP 1 Score: 211.846 bits (538), Expect = 1.185e-55
Identity = 101/147 (68.71%), Postives = 118/147 (80.27%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQCSYD +GNSVPTILL MQ+RLY++GGLKAEGIFRINP+NG+EEH+R QLN G+VP  IDVHCLAGLIKAWFRELP+GVLD L+PE+V+ CNT                A+L+WAI LMADVV    +NKM+ARN+AMVFAPNMT
Sbjct:  154 SMQCSYDDRGNSVPTILLRMQKRLYTEGGLKAEGIFRINPDNGKEEHVRRQLNCGVVPRGIDVHCLAGLIKAWFRELPTGVLDVLTPEQVMRCNTEEDCSRLVILLPPVESAILDWAIGLMADVVEHEQFNKMNARNVAMVFAPNMT 300

[Close]

BLAST of FG531753 vs. TAIR peptide
Match: AT3G11490.1 (| Symbols: | rac GTPase activating protein | chr3:3617523-3619567 REVERSE LENGTH=435)

HSP 1 Score: 195.667 bits (496), Expect = 8.788e-51
Identity = 96/147 (65.31%), Postives = 115/147 (78.23%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQ SYD++GN VPTILL+MQ  LYS+GGL+ EGIFRIN ENG+EE++RE+LN GI+P++IDVHCLA LIKAWFRELPSGVLD LSPE+V+E  +                +LL+WAI+LMADVV     NKM+ARNIAMVFAPNMT
Sbjct:  142 SMQLSYDTRGNIVPTILLMMQSHLYSRGGLRVEGIFRINGENGQEEYIREELNKGIIPDNIDVHCLASLIKAWFRELPSGVLDSLSPEQVMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNMT 288

[Close]

BLAST of FG531753 vs. TAIR peptide
Match: AT5G22400.1 (| Symbols: | Rho GTPase activating protein with PAK-box/P21-Rho-binding domain | chr5:7423050-7425335 REVERSE LENGTH=466)

HSP 1 Score: 194.126 bits (492), Expect = 2.557e-50
Identity = 98/147 (66.67%), Postives = 111/147 (75.51%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQ SYDS+GN VPTILLLMQ  LYSQGGL+AEGIFR+  EN EEE +REQLN G +P  IDVHCLAGLIKAWFRELP+ VLD LSPE+V++C T                ALL+WAI+LMADVV     NKM++RNIAMVFAPNMT
Sbjct:  166 SMQLSYDSRGNCVPTILLLMQNCLYSQGGLQAEGIFRLTAENSEEEAVREQLNRGFIPERIDVHCLAGLIKAWFRELPTSVLDSLSPEQVMQCQTEEENVELVRLLPPTEAALLDWAINLMADVVQYEHLNKMNSRNIAMVFAPNMT 312

[Close]

BLAST of FG531753 vs. TAIR peptide
Match: AT1G08340.1 (| Symbols: | Rho GTPase activating protein with PAK-box/P21-Rho-binding domain | chr1:2631308-2632669 FORWARD LENGTH=331)

HSP 1 Score: 186.422 bits (472), Expect = 5.331e-48
Identity = 91/147 (61.90%), Postives = 110/147 (74.83%), Query Frame = 3

Query:    3 SMQCSYDSKGNSVPTILLLMQERLYSQGGLKAEGIFRINPENGEEEHLREQLNSGIVPNDIDVHCLAGLIKAWFRELPSGVLDGLSPEEVLECNTXXXXXXXXXXXXXXXXALLNWAIDLMADVVVEADYNKMDARNIAMVFAPNMT 443
            SMQ SYDS+GN VP ILLL+Q RLY QGGL+AEG+FRI  EN EEE +REQLN GI+P+ IDVHCLAGLIKAWFRELP GVLD L  E+V++C +                +LLNWAI+LMADV+     NKM++RN+A+VFAPNM+
Sbjct:   52 SMQLSYDSRGNCVPVILLLLQSRLYDQGGLQAEGVFRITGENSEEEFVREQLNKGIIPDGIDVHCLAGLIKAWFRELPRGVLDPLPSEQVMQCESDEDFVKVVRLLPQTEASLLNWAINLMADVIQFEHVNKMNSRNLALVFAPNMS 198

The following BLAST results are available for this feature:

BLAST of FG531753 vs. TrEMBL
BLAST of FG531753 vs. SwissProt
BLAST of FG531753 vs. TAIR peptide

BLAST of FG531753 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O82460_LOTJA	1.352e-61	79.59	Rac GTPase activating protein 3 (Fragment) OS=Lotu...	[more]
B9S3Y4_RICCO	8.761e-61	78.91	Gtpase activating protein, putative OS=Ricinus com...	[more]
B9I9Y6_POPTR	1.265e-59	77.55	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q9ZNR7_ARATH	2.818e-59	78.23	Putative rac GTPase activating protein OS=Arabidop...	[more]
D7M3A3_ARALY	1.071e-58	77.55	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TP55_VITVI	2.386e-58	76.87	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5YPA0_SORBI	1.309e-56	72.79	Putative uncharacterized protein Sb08g016850 OS=So...	[more]
Q6UQ73_ORYSJ	2.233e-56	72.79	Os12g0533400 protein (Fragment) OS=Oryza sativa su...	[more]
Q2QPD6_ORYSJ	2.233e-56	72.79	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8BM94_ORYSI	1.108e-55	72.11	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG531753 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GACA_DICDI	8.733e-19	35.00	Rho GTPase-activating protein gacA OS=Dictyosteliu...	[more]
GACO_DICDI	5.132e-11	32.35	Rho GTPase-activating protein gacO OS=Dictyosteliu...	[more]
RGA2_SCHPO	1.070e-8	26.85	Probable Rho-type GTPase-activating protein 2 OS=S...	[more]
RHG24_RAT	3.113e-8	28.19	Rho GTPase-activating protein 24 OS=Rattus norvegi...	[more]
RHG24_MOUSE	3.113e-8	28.19	Rho GTPase-activating protein 24 OS=Mus musculus G...	[more]
MGP4_DICDI	4.066e-8	26.76	Mental retardation GTPase activating protein homol...	[more]
RHG23_MOUSE	1.545e-7	25.79	Rho GTPase-activating protein 23 OS=Mus musculus G...	[more]
RG68F_DROME	1.545e-7	27.78	Rho GTPase-activating protein 68F OS=Drosophila me...	[more]
GACY_DICDI	2.018e-7	28.79	Rho GTPase-activating protein gacY OS=Dictyosteliu...	[more]
RHG23_HUMAN	4.496e-7	25.16	Rho GTPase-activating protein 23 OS=Homo sapiens G...	[more]

BLAST of FG531753 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G03100.1	1.887e-61	78.23	\| Symbols: \| Rho GTPase activating protein with P...	[more]
AT2G46710.1	1.185e-55	68.71	\| Symbols: \| Rho GTPase activating protein with P...	[more]
AT3G11490.1	8.788e-51	65.31	\| Symbols: \| rac GTPase activating protein \| chr3...	[more]
AT5G22400.1	2.557e-50	66.67	\| Symbols: \| Rho GTPase activating protein with P...	[more]
AT1G08340.1	5.331e-48	61.90	\| Symbols: \| Rho GTPase activating protein with P...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000198	Rho GTPase-activating protein domain	GENE3D	1.10.555.10	no description	coord: 6..146 score: 8
IPR000198	Rho GTPase-activating protein domain	PFAM	PF00620	RhoGAP	coord: 14..146 score: 4.9
IPR000198	Rho GTPase-activating protein domain	SMART	SM00324	no description	coord: 11..147 score: 6.6
IPR000198	Rho GTPase-activating protein domain	PROFILE	PS50238	RHOGAP	coord: 1..147 score: 24
IPR008936	Rho GTPase activation protein	SUPERFAMILY	48350	GTPase activation domain, GAP	coord: 7..146 score: 1.8
None	No IPR available	PANTHER	PTHR23177	MKIAA1688 PROTEIN	coord: 1..147 score: 7.9e
None	No IPR available	PANTHER	PTHR23177:SF2	GTPASE ACTIVATING PROTEIN	coord: 1..147 score: 7.9e
None	No IPR available	SEG	seg	seg	coord: 96..111 score
None	No IPR available	SEG	seg	seg	coord: 20..42 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531753 ID=FG531753; Name=FG531753; organism=Pisum sativum; type=EST; length=444bp
AATCAATGCAATGTTCTTATGATTCAAAAGGAAACAGTGTCCCCACTATT
CTGCTACTGATGCAGGAAAGGTTATACTCACAGGGAGGACTGAAGGCTGA
AGGAATATTCCGTATAAATCCGGAAAACGGTGAAGAGGAACATTTGAGAG
AGCAATTGAATAGCGGCATTGTGCCGAATGACATTGATGTTCATTGTTTG
GCTGGTTTGATAAAAGCATGGTTTAGGGAACTTCCTTCTGGAGTTTTAGA
TGGACTTTCGCCGGAGGAAGTTCTTGAATGCAACACTGAAGAAGAATCTG
TTGAGCTTGTGAAGGAGCTGAAACCAGTTGAATCAGCTCTGCTTAATTGG
GCTATTGATCTCATGGCTGATGTTGTAGTTGAAGCCGACTATAATAAAAT
GGATGCTAGAAATATTGCAATGGTTTTCGCTCCAAATATGACTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure

Vocabulary: **Biological Process**
Term	Definition
GO:0007165	signal transduction

Vocabulary: **INTERPRO**
Term	Definition
IPR000198	RhoGAP_dom
IPR008936	Rho_GTPase_activation_prot

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