FG531986

EST Overview


Name	FG531986
Unique Name	FG531986
Type	EST
Organism	Pisum sativum (pea)
Sequence length	489

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG531986 vs. TrEMBL
Match: B9GLN3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_639731 PE=3 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 5.600e-68
Identity = 122/159 (76.73%), Postives = 141/159 (88.68%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDIT+HGPHQAILYPIISLRD GLGAR++VEK+ELTMI++A  YGVKAC G++CETGVWVG+RKIGAIGVRIS+GITSHGLA N+DPDL YF+HIVPCGIADKEVTSLR+ETG  LP+EE+I E+LISCFARIFG++NLIWKE    L    + E
Sbjct:   77 TQRGGDITYHGPHQAILYPIISLRDIGLGARKYVEKLELTMIDMASQYGVKACAGKKCETGVWVGDRKIGAIGVRISSGITSHGLAFNVDPDLDYFKHIVPCGIADKEVTSLRRETGTVLPSEEVIHEQLISCFARIFGFNNLIWKENEPTLLDNGKPE 235

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BLAST of FG531986 vs. TrEMBL
Match: D7KE60_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_311551 PE=4 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 2.692e-62
Identity = 113/152 (74.34%), Postives = 130/152 (85.53%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASIL 457
            TQRGGDITFHGPHQAILYPIISLR  G GAR +VE +E +MIE A IYGVKA  G +CETGVWVG+RKIGAIGVRIS+GITSHGLA NIDPDLKYF HIVPCGIADKEVTSLR+ET   LP+EE+I E+L+SC A+ F YD+++WKE  S++
Sbjct:  175 TQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLAFNIDPDLKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHEQLVSCLAKAFSYDDVVWKEDPSVI 326

[Close]

BLAST of FG531986 vs. TrEMBL
Match: B9SJ07_RICCO (Lipoate-protein ligase B, putative OS=Ricinus communis GN=RCOM_0597510 PE=3 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 1.934e-52
Identity = 99/129 (76.74%), Postives = 115/129 (89.15%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEE 388
            TQRGGDIT+HGP QA+LYPIISLRD GLGAR +VEK+E TMIELA +YGVKAC GQ+ ETGVWVG+RKIGAIGVRIS+GITSHGLA NIDP+L YF++IVPCGI DKEVTSLR+ETG  LP++E++  E
Sbjct:   77 TQRGGDITYHGPRQAVLYPIISLRDIGLGARNYVEKLESTMIELALLYGVKACAGQKGETGVWVGDRKIGAIGVRISSGITSHGLAFNIDPNLNYFKNIVPCGIVDKEVTSLRRETGVVLPSDEVLWNE 205

[Close]

BLAST of FG531986 vs. TrEMBL
Match: Q10M64_ORYSJ (Lipoate-protein ligase, putative, expressed OS=Oryza sativa subsp. japonica GN=Os03g0321800 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.525e-52
Identity = 96/147 (65.31%), Postives = 120/147 (81.63%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKE 442
            T+RGGD+TFHGP QA+LYPI+SLR  GLGARR+VE +E  MIE+A +YGV+ACPG   ETGVWVG+RKIGAIGVRIS+G T HGLA NIDPDL +F HIVPCGIADKEVTSLR+E   +LP +E+I ++L+   AR F + ++ +K+
Sbjct:   79 TERGGDVTFHGPRQAVLYPILSLRAIGLGARRYVEGLESAMIEVAALYGVQACPGAAGETGVWVGDRKIGAIGVRISSGFTCHGLAFNIDPDLGFFEHIVPCGIADKEVTSLRREAAVELPPDEVIHDQLVQSLARTFCFSDVEFKD 225

[Close]

BLAST of FG531986 vs. TrEMBL
Match: A2XG45_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_11351 PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.220e-51
Identity = 95/147 (64.63%), Postives = 119/147 (80.95%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKE 442
            T+RGGD+TFHGP QA+LYPI+SLR  GLGARR+VE +E  MIE+A +YGV+A PG   ETGVWVG+RKIGAIGVRIS+G T HGLA NIDPDL +F HIVPCGIADKEVTSLR+E   +LP +E+I ++L+   AR F + ++ +K+
Sbjct:   79 TERGGDVTFHGPRQAVLYPILSLRAIGLGARRYVEGLESAMIEVAALYGVQARPGAAGETGVWVGDRKIGAIGVRISSGFTCHGLAFNIDPDLGFFEHIVPCGIADKEVTSLRREAAVELPPDEVIHDQLVQSLARTFCFSDVDFKD 225

[Close]

BLAST of FG531986 vs. TrEMBL
Match: D7SLB9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025351001 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.295e-47
Identity = 100/159 (62.89%), Postives = 109/159 (68.55%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDITFHGPHQAILYPIISLRD GLGAR +                                 RKIGAIGVRIS+GITSHGLA NIDPDL YF+HIVPCGIADKEVTSLR+ET   LPTEE+I E+LISCFAR+F + +LIWKE A IL    E E
Sbjct:  149 TQRGGDITFHGPHQAILYPIISLRDIGLGARNY---------------------------------RKIGAIGVRISSGITSHGLAFNIDPDLNYFKHIVPCGIADKEVTSLRRETDLVLPTEEVIHEQLISCFARLFCFSDLIWKEDAPILPDNGEAE 274

[Close]

BLAST of FG531986 vs. TrEMBL
Match: C5X1S8_SORBI (Putative uncharacterized protein Sb01g036860 OS=Sorghum bicolor GN=Sb01g036860 PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.704e-46
Identity = 90/149 (60.40%), Postives = 115/149 (77.18%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKET--GFDLPTEEIIQEELISCFARIFGYDNLIWKE 442
            T+RGGD+TFHGP QA+LYP++SLR   LGARR+VE +E  MI++A ++GV A PG   ETGVWVG+RKIGAIGVRIS+G T HGLA NIDPDL YF HIVPCGIA K VTSLR+E   G +LP + +I ++L+ C A   G+ ++ +K+
Sbjct:   81 TERGGDVTFHGPRQAVLYPVLSLRSLRLGARRYVEGLESAMIQVAALHGVFARPGDPGETGVWVGDRKIGAIGVRISSGFTWHGLAFNIDPDLGYFEHIVPCGIAGKGVTSLRREVGDGVELPDDGVIHDQLVRCLATTLGFTDVEFKD 229

[Close]

BLAST of FG531986 vs. TrEMBL
Match: B9GYV9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555282 PE=4 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 6.893e-42
Identity = 83/119 (69.75%), Postives = 99/119 (83.19%), Query Frame = 2

Query:  122 MIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            MIE+A ++GVKA   ++CETGVWVG+RKIGAIGV IS+ ITSHGLA NIDPDL YF+HIVPCGIADK+VTSLR+ETG  LP++E+I E LISCFARIFG+ NLIWKE  S+L    + E
Sbjct:    1 MIEMASLHGVKASARKKCETGVWVGDRKIGAIGVCISSRITSHGLAFNIDPDLDYFKHIVPCGIADKQVTSLRRETGTVLPSDEVINEPLISCFARIFGFKNLIWKENKSMLLDNGKPE 119

[Close]

BLAST of FG531986 vs. TrEMBL
Match: A5ATV4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030852 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 4.940e-40
Identity = 91/159 (57.23%), Postives = 100/159 (62.89%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDITFHGPHQAILYPIISLRD GLGAR +VEK+ELTMIELA +YGVKA  G                                          HIVPCGIADKEVTSLR+ET   LPTEE+I E+LISCFAR+FG+ +LIWKE A IL    E E
Sbjct:   77 TQRGGDITFHGPHQAILYPIISLRDIGLGARNYVEKLELTMIELASLYGVKARAG-----------------------------------------GHIVPCGIADKEVTSLRRETDLVLPTEEVIHEQLISCFARLFGFSDLIWKEDAPILPDNGEAE 194

[Close]

BLAST of FG531986 vs. TrEMBL
Match: A9U205_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_26758 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 1.345e-37
Identity = 83/140 (59.29%), Postives = 103/140 (73.57%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWV-GERKIGAIGVRISNG-ITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIF 415
            T+RGGDIT+HGP QA+LYPI+ LR+  +G R FVE++E  M+++   +GV A      ETGVWV G RKIGAIGV++S+G ITSHGLA NI+PDL YF HIVPCGI DKEVTSLRKE    +P  E + E+LI   A+ F
Sbjct:   38 TERGGDITYHGPGQAVLYPILGLRELRVGVRAFVERLEDVMVDVCKFHGVNARGRMDKETGVWVEGLRKIGAIGVKVSSGSITSHGLAFNINPDLVYFDHIVPCGIRDKEVTSLRKEISPVVPDAEEVTEQLIQSLAKQF 177

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_ARATH (Octanoyltransferase OS=Arabidopsis thaliana GN=LIP2 PE=1 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 2.594e-63
Identity = 113/159 (71.07%), Postives = 132/159 (83.02%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDITFHGPHQAILYPIISLR  G GAR +VE +E +MIE A IYGVKA  G +CETGVWVG+RKIGAIGVRIS+GITSHGLA NIDPD+KYF HIVPCGIADKEVTSLR+ET   LP+EE+I E+L+SC A+ F YD+++WKE  S++    + E
Sbjct:   77 TQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHEQLVSCLAKAFSYDDVVWKEDPSLILDTQDKE 235

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_SOLUE (Octanoyltransferase OS=Solibacter usitatus (strain Ellin6076) GN=lipB PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.487e-26
Identity = 69/151 (45.70%), Postives = 92/151 (60.93%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASI 454
            T RGGD+T+HGP Q + YPI+ LRD       +V  +E  +I+  G YG++A    +  TGVWVGERKI AIGV +S  +TSHG A N+  DL YF++IVPCG+  K VTS+    G     +E + E     FARIF ++ L    GAS+
Sbjct:   74 TNRGGDVTYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGDYGIEAGRIPKL-TGVWVGERKIAAIGVHLSRWVTSHGFALNVSTDLSYFQYIVPCGLT-KPVTSM-AALGVRASLDE-VGERFTRHFARIFDFEML---PGASL 217

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPT2_DANRE (Putative lipoyltransferase 2, mitochondrial OS=Danio rerio GN=lipt2 PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 2.536e-26
Identity = 64/138 (46.38%), Postives = 87/138 (63.04%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIF 415
            T RGG ITFHGP Q + YPI++L       R +V ++E T+I++ G +G+KA      +TGVWVG+ KI AIG+     ITSHGLA N + D+ +F +IVPCGI  K VTSL +E   D+P +E I  +L+  F   F
Sbjct:   79 TNRGGLITFHGPGQLVCYPILNLGCFKKSVRWYVCELERTVIKMCGKFGIKASTSP--DTGVWVGDNKICAIGIHCGRYITSHGLALNCNTDMSWFDNIVPCGIVGKGVTSLSQELERDVPPDEAI-PKLLEAFTEQF 213

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_ALKMQ (Octanoyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=lipB PE=3 SV=2)

HSP 1 Score: 117.087 bits (292), Expect = 4.325e-26
Identity = 53/141 (37.59%), Postives = 86/141 (60.99%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYD 424
            T RGGD+T+HGP Q + YPI+++R + +G ++FVE +E  +I L                GVWVG  KI AIG+ +  G++ HG A N+  +L +F+ +VPCGIAD+ VTS+ +  G+ +    +  + +++ F+ +F YD
Sbjct:   73 TDRGGDVTYHGPGQIVGYPIVNIRKSKIGVKKFVENLEEVLIRLLDEKYHITATRSSVNPGVWVGANKIAAIGLAVKRGVSMHGFALNVSTNLAHFKFLVPCGIADRGVTSIEEILGYPVDF-HVANQHVLAYFSEVFNYD 212

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_BACTN (Octanoyltransferase OS=Bacteroides thetaiotaomicron GN=lipB PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 5.649e-26
Identity = 60/130 (46.15%), Postives = 86/130 (66.15%), Query Frame = 2

Query:    8 RGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVG-----ERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQ 382
            RGGDIT+HGP Q + YPI++L +  LG + +V  +E  +I +   YG++A   ++  TGVW+       RKI AIGVR S+ +T HGLA N++ DL+YF +I PCG  DK VTSLR+E   ++P +E+ Q
Sbjct:   80 RGGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIRVCASYGIEAGRLEKA-TGVWLEGSTPRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLRQELKHEVPMDEVKQ 208

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_BACFN (Octanoyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=lipB PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 1.644e-25
Identity = 60/128 (46.88%), Postives = 83/128 (64.84%), Query Frame = 2

Query:    8 RGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVG-----ERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEI 376
            RGGDIT+HGP Q + YPI++L + GLG + +V  +E  +I +   YGV A   ++  TGVW+       RKI AIGVR S+ +T HGLA N++ DL+YF +I PCG  DK VTSL++E G  +   E+
Sbjct:   80 RGGDITYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAGRLEKA-TGVWLEGDTSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLQQELGHSIDMAEV 206

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_THET8 (Octanoyltransferase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=lipB PE=1 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.147e-25
Identity = 61/138 (44.20%), Postives = 85/138 (61.59%), Query Frame = 2

Query:    5 QRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFG 418
            +RGGD+T+HGP Q + YPI  +   G   RRF+ +IE  ++ +A  YG+ A P      GVWVGE K+ AIGV +  G++ HG A N++ DL  F  IVPCG+  K VTSL K  G  +P EE  +  +++ FA +FG
Sbjct:   74 ERGGDVTYHGPGQLVGYPIFPV---GREVRRFLRQIEEAIVRVAAGYGISAYPTPGY-AGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTDLNDFTVIVPCGLKGKGVTSLEKLLGRKVPMEE-AKARVVAAFAEVFG 206

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_THET2 (Octanoyltransferase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=lipB PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.147e-25
Identity = 61/138 (44.20%), Postives = 85/138 (61.59%), Query Frame = 2

Query:    5 QRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFG 418
            +RGGD+T+HGP Q + YPI  +   G   RRF+ +IE  ++ +A  YG+ A P      GVWVGE K+ AIGV +  G++ HG A N++ DL  F  IVPCG+  K VTSL K  G  +P EE  +  +++ FA +FG
Sbjct:   74 ERGGDVTYHGPGQLVGYPIFPV---GREVRRFLRQIEEAIVRVAAGYGISAYPTPGY-AGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTDLNDFTVIVPCGLKGKGVTSLEKLLGRKVPMEE-AKARVVAAFAEVFG 206

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_BACFR (Octanoyltransferase OS=Bacteroides fragilis GN=lipB PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.804e-25
Identity = 60/128 (46.88%), Postives = 83/128 (64.84%), Query Frame = 2

Query:    8 RGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVG-----ERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEI 376
            RGGDIT+HGP Q + YPI++L + GLG + +V  +E  +I +   YGV A   ++  TGVW+       RKI AIGVR S+ +T HGLA N++ DL+YF +I PCG  DK VTSL++E G  +   E+
Sbjct:   80 RGGDITYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAGRLEKA-TGVWLEGDTSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLQQELGRSIDMAEV 206

[Close]

BLAST of FG531986 vs. SwissProt
Match: LIPB_ACIBL (Octanoyltransferase OS=Acidobacteria bacterium (strain Ellin345) GN=lipB PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 4.782e-25
Identity = 69/160 (43.12%), Postives = 90/160 (56.25%), Query Frame = 2

Query:    8 RGGDITFHGPHQAILYPIISLRDTG--LGARRFVEKIELTMIELAGIYGVKACPGQRCE--TGVWV---GERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGY---DNLIWKEGASIL 457
            RGGD+TFHGP Q + YPI  LR     +G   +V +IE  +I   G  G+   P  R E  TGVW     + KI AIGV IS  +TSHG A N+  DL YF+ IVPCGI+DK VTS++ E G  L  EE+     +    R FG+   + ++W E  + L
Sbjct:   74 RGGDVTFHGPGQLVGYPIFDLRSLNPKIGVIEYVRRIEEVLIRSCGDLGI---PTTRVEGLTGVWTVNEPQAKIAAIGVHISRAVTSHGFALNVTTDLDYFKLIVPCGISDKPVTSMQHELGKSLTLEEV-----MPVITRNFGFIFKEQVLWLESLNDL 225

[Close]

BLAST of FG531986 vs. TAIR peptide
Match: AT1G04640.2 (| Symbols: LIP2 | lipoyltransferase 2 | chr1:1292541-1293248 FORWARD LENGTH=235)

HSP 1 Score: 240.736 bits (613), Expect = 2.968e-64
Identity = 113/159 (71.07%), Postives = 132/159 (83.02%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDITFHGPHQAILYPIISLR  G GAR +VE +E +MIE A IYGVKA  G +CETGVWVG+RKIGAIGVRIS+GITSHGLA NIDPD+KYF HIVPCGIADKEVTSLR+ET   LP+EE+I E+L+SC A+ F YD+++WKE  S++    + E
Sbjct:   77 TQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHEQLVSCLAKAFSYDDVVWKEDPSLILDTQDKE 235

[Close]

BLAST of FG531986 vs. TAIR peptide
Match: AT1G04640.1 (| Symbols: LIP2 | lipoyltransferase 2 | chr1:1292541-1293248 FORWARD LENGTH=235)

HSP 1 Score: 240.736 bits (613), Expect = 2.968e-64
Identity = 113/159 (71.07%), Postives = 132/159 (83.02%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIELAGIYGVKACPGQRCETGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRKETGFDLPTEEIIQEELISCFARIFGYDNLIWKEGASILSGXNETE 478
            TQRGGDITFHGPHQAILYPIISLR  G GAR +VE +E +MIE A IYGVKA  G +CETGVWVG+RKIGAIGVRIS+GITSHGLA NIDPD+KYF HIVPCGIADKEVTSLR+ET   LP+EE+I E+L+SC A+ F YD+++WKE  S++    + E
Sbjct:   77 TQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHEQLVSCLAKAFSYDDVVWKEDPSLILDTQDKE 235

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BLAST of FG531986 vs. TAIR peptide
Match: AT4G31050.1 (| Symbols: | Biotin/lipoate A/B protein ligase family | chr4:15114345-15115443 FORWARD LENGTH=278)

HSP 1 Score: 105.916 bits (263), Expect = 1.141e-23
Identity = 60/148 (40.54%), Postives = 91/148 (61.49%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIE-LAGIYGVKACPGQRCE--TGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLR-----KETGF--DLPTEEIIQEELISCFARIF 415
            T+RGG++T+HGP Q ++YPII+LR+  +    ++  +E  +I  L+  + +KA    R +  TGVWVG +K+ AIG+R+S  IT HGLA N+  DL  F  IVPCGI D++V +++      E G   DL   +I+ E L+  F+  F
Sbjct:  119 TERGGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRVLSSTFSIKA---SRLDGLTGVWVGNQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGIRDRKVGNIKGLLEDGEHGMVDDLRLIDIVHESLLKEFSEAF 263

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BLAST of FG531986 vs. TAIR peptide
Match: AT1G47578.1 (| Symbols: | Biotin/lipoate A/B protein ligase family | chr1:17484365-17485470 FORWARD LENGTH=280)

HSP 1 Score: 102.449 bits (254), Expect = 1.262e-22
Identity = 59/149 (39.60%), Postives = 90/149 (60.40%), Query Frame = 2

Query:    2 TQRGGDITFHGPHQAILYPIISLRDTGLGARRFVEKIELTMIE-LAGIYGVKACPGQRCE--TGVWVGERKIGAIGVRISNGITSHGLASNIDPDLKYFRHIVPCGIADKEVTSLRK--ETG------FDLPTEEIIQEELISCFARIF 415
            T+RGG++T+HGP Q ++YPII+LR+  +    ++ K+E  +I  L+  + + A    R +  TGVWVG +K+ AIG+R+S  +T HGLA N+  DL  F  IVPCGI ++ V S++   E G       DL   +I  E L+  F+ +F
Sbjct:  121 TERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRKLEEVVIRVLSSAFAINA---SRLDGFTGVWVGNKKMAAIGIRVSKWMTYHGLALNVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHYNKLEDLQLLDIAHESLLKEFSEVF 266

The following BLAST results are available for this feature:

BLAST of FG531986 vs. TrEMBL
BLAST of FG531986 vs. SwissProt
BLAST of FG531986 vs. TAIR peptide

BLAST of FG531986 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GLN3_POPTR	5.600e-68	76.73	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7KE60_ARALY	2.692e-62	74.34	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9SJ07_RICCO	1.934e-52	76.74	Lipoate-protein ligase B, putative OS=Ricinus comm...	[more]
Q10M64_ORYSJ	2.525e-52	65.31	Lipoate-protein ligase, putative, expressed OS=Ory...	[more]
A2XG45_ORYSI	6.220e-51	64.63	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7SLB9_VITVI	9.295e-47	62.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5X1S8_SORBI	2.704e-46	60.40	Putative uncharacterized protein Sb01g036860 OS=So...	[more]
B9GYV9_POPTR	6.893e-42	69.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5ATV4_VITVI	4.940e-40	57.23	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A9U205_PHYPA	1.345e-37	59.29	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]

BLAST of FG531986 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LIPB_ARATH	2.594e-63	71.07	Octanoyltransferase OS=Arabidopsis thaliana GN=LIP...	[more]
LIPB_SOLUE	1.487e-26	45.70	Octanoyltransferase OS=Solibacter usitatus (strain...	[more]
LIPT2_DANRE	2.536e-26	46.38	Putative lipoyltransferase 2, mitochondrial OS=Dan...	[more]
LIPB_ALKMQ	4.325e-26	37.59	Octanoyltransferase OS=Alkaliphilus metalliredigen...	[more]
LIPB_BACTN	5.649e-26	46.15	Octanoyltransferase OS=Bacteroides thetaiotaomicro...	[more]
LIPB_BACFN	1.644e-25	46.88	Octanoyltransferase OS=Bacteroides fragilis (strai...	[more]
LIPB_THET8	2.147e-25	44.20	Octanoyltransferase OS=Thermus thermophilus (strai...	[more]
LIPB_THET2	2.147e-25	44.20	Octanoyltransferase OS=Thermus thermophilus (strai...	[more]
LIPB_BACFR	2.804e-25	46.88	Octanoyltransferase OS=Bacteroides fragilis GN=lip...	[more]
LIPB_ACIBL	4.782e-25	43.13	Octanoyltransferase OS=Acidobacteria bacterium (st...	[more]

BLAST of FG531986 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G04640.2	2.968e-64	71.07	\| Symbols: LIP2 \| lipoyltransferase 2 \| chr1:12925...	[more]
AT1G04640.1	2.968e-64	71.07	\| Symbols: LIP2 \| lipoyltransferase 2 \| chr1:12925...	[more]
AT4G31050.1	1.141e-23	40.54	\| Symbols: \| Biotin/lipoate A/B protein ligase fa...	[more]
AT1G47578.1	1.262e-22	39.60	\| Symbols: \| Biotin/lipoate A/B protein ligase fa...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004143	Biotin/lipoate A/B protein ligase	PFAM	PF03099	BPL_LplA_LipB	coord: 1..92 score: 9.5
IPR020605	Octanoyltransferase, conserved site	PROSITE	PS01313	LIPB	coord: 3..18 score
None	No IPR available	GENE3D	3.90.1550.10	no description	coord: 3..139 score: 0.0
None	No IPR available	PANTHER	PTHR10993	LIPOATE-PROTEIN LIGASE B	coord: 1..152 score: 6.2
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 1..143 score: 2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531986 ID=FG531986; Name=FG531986; organism=Pisum sativum; type=EST; length=489bp
CACACAACGAGGAGGAGACATTACATTTCACGGTCCACACCAGGCCATTT
TATATCCTATCATTTCGCTCCGTGACACGGGACTTGGTGCTAGAAGATTT
GTAGAAAAGATTGAGTTAACTATGATTGAATTGGCAGGTATATACGGCGT
CAAAGCTTGTCCTGGGCAGAGGTGTGAGACAGGAGTGTGGGTTGGAGAAA
GGAAGATAGGGGCAATTGGTGTTCGGATATCAAATGGAATCACTTCTCAT
GGTTTGGCATCTAACATTGACCCTGATTTAAAGTACTTCAGGCACATTGT
TCCGTGTGGAATTGCAGATAAAGAGGTGACATCTTTGAGAAAGGAGACAG
GTTTTGACCTTCCCACAGAAGAAATAATACAAGAGGAGTTGATTTCATGT
TTTGCAAGAATCTTTGGTTATGATAACCTGATCTGGAAAGAGGGTGCTTC
TATATTGTCTGGTAANAATGAAACTGAATGAGACAGCTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0016415	octanoyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006464	cellular protein modification process
GO:0009107	lipoate biosynthetic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR004143	BPL_LPL_catalytic
IPR020605	Octanoyltransferase_CS

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