FG532994

EST Overview


Name	FG532994
Unique Name	FG532994
Type	EST
Organism	Pisum sativum (pea)
Sequence length	536

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG532994 vs. TrEMBL
Match: D7SGZ8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008554001 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 2.130e-37
Identity = 75/94 (79.79%), Postives = 85/94 (90.43%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDSNAG 288
            G++S ADL  VLKLKKELC AQS NESHVPD LLERLV+DT+EFPPVCA++GGILGQEVIKAISGKGDP+KNFF+FDA DGKG+IEDIS+ N G
Sbjct:  229 GEISIADLPGVLKLKKELCEAQSFNESHVPDALLERLVSDTSEFPPVCAILGGILGQEVIKAISGKGDPLKNFFFFDAIDGKGIIEDISNPNPG 322

[Close]

BLAST of FG532994 vs. TrEMBL
Match: A5BME4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022139 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.604e-35
Identity = 75/101 (74.26%), Postives = 85/101 (84.16%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQ-------SLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDSNAG 288
            G++S ADL  VLKLKKELC AQ       S NESHVPD LLERLV+DT+EFPPVCA++GGILGQEVIKAISGKGDP+KNFF+FDA DGKG+IEDIS+ N G
Sbjct:  187 GEISIADLPGVLKLKKELCEAQYYLVQSQSFNESHVPDALLERLVSDTSEFPPVCAILGGILGQEVIKAISGKGDPLKNFFFFDAIDGKGIIEDISNPNPG 287

[Close]

BLAST of FG532994 vs. TrEMBL
Match: C6TF08_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.911e-34
Identity = 70/89 (78.65%), Postives = 80/89 (89.89%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+VS AD S +LKLKKE+CT QSLNES VPD+LL+RLV +  EFPPVCA+IGGILGQEVIKAISGKGDP+KNFF+FDA DGKG+IEDIS
Sbjct:  231 GEVSIADFSGLLKLKKEICTTQSLNESQVPDSLLKRLVTNATEFPPVCAIIGGILGQEVIKAISGKGDPLKNFFFFDAFDGKGIIEDIS 319

[Close]

BLAST of FG532994 vs. TrEMBL
Match: B9IFE2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_575384 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 6.415e-34
Identity = 70/92 (76.09%), Postives = 79/92 (85.87%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDSN 282
            G++   DL  VLKLKKELC AQSLNESH+P+ LLERLV    EFPPVCA+IGGILGQEVIKAISGKGDP+KNFF+FD+ DGKG+IEDISD N
Sbjct:  229 GEICIEDLPAVLKLKKELCEAQSLNESHIPNALLERLVMGAREFPPVCAIIGGILGQEVIKAISGKGDPLKNFFFFDSVDGKGIIEDISDPN 320

[Close]

BLAST of FG532994 vs. TrEMBL
Match: B9I3U4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_823422 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 4.158e-33
Identity = 70/92 (76.09%), Postives = 78/92 (84.78%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDSN 282
            G++   DL  VLKLKKELC AQS+NESHVPDTLLERLV    EFPPVCA+IGG LGQEVIKAIS KGDP+KNFF FDA+DGKG+IEDIS+ N
Sbjct:  229 GEICIEDLPAVLKLKKELCEAQSVNESHVPDTLLERLVMGAKEFPPVCAIIGGTLGQEVIKAISSKGDPVKNFFIFDATDGKGMIEDISNPN 320

[Close]

BLAST of FG532994 vs. TrEMBL
Match: Q0WNE6_ARATH (Ubiquitin activating enzyme OS=Arabidopsis thaliana GN=At5g50680 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.980e-31
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  127 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 215

[Close]

BLAST of FG532994 vs. TrEMBL
Match: D7MQ99_ARALY (Sumo activating enzyme 1b OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495124 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.980e-31
Identity = 64/90 (71.11%), Postives = 76/90 (84.44%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISD 276
            G+ S +DL  VLKLKKELC   S++E H+PD+LLERLV+   EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S+
Sbjct:  229 GECSLSDLPRVLKLKKELCEGNSVSEKHIPDSLLERLVSGNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLSN 318

[Close]

BLAST of FG532994 vs. TrEMBL
Match: B9RAD1_RICCO (DNA damage tolerance protein rad31, putative OS=Ricinus communis GN=RCOM_1505590 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.132e-30
Identity = 65/88 (73.86%), Postives = 74/88 (84.09%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDI 270
            G++   DL  VL LKKELC AQSL+ESH+P+ LLERLV    EFPPVCA+IGGILGQEVIK ISGKGDP+KNFF+FDA DGKG+IEDI
Sbjct:  229 GEIYIKDLPAVLSLKKELCEAQSLSESHIPNALLERLVMGGREFPPVCAIIGGILGQEVIKVISGKGDPLKNFFFFDAMDGKGIIEDI 316

[Close]

BLAST of FG532994 vs. TrEMBL
Match: D7MGL4_ARALY (Sumo-activating enzyme 1A OS=Arabidopsis lyrata subsp. lyrata GN=SAE1A PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.294e-30
Identity = 60/91 (65.93%), Postives = 77/91 (84.62%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDS 279
            G+ S  DL+ VL++KK+LC A S++ESH+PD+LL RL+  T EFPP CA++GGIL QEVIKA+SGKGDP+KNFFY+D  DGKGV+EDIS+S
Sbjct:  229 GECSLLDLARVLEIKKQLCEANSVSESHIPDSLLGRLITGTTEFPPACAIVGGILAQEVIKAVSGKGDPLKNFFYYDGEDGKGVMEDISNS 319

[Close]

BLAST of FG532994 vs. TrEMBL
Match: O65078_PICMA (Probable ubiquitin activating enzyme 2 (Fragment) OS=Picea mariana GN=Sb53 PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.530e-27
Identity = 62/93 (66.67%), Postives = 75/93 (80.65%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLV-ADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDSN 282
            G VS ADL  VL L+K+LC  Q L+ES +PD LLER++ A T + PPVCA+IGGILGQEVIKA+S KGDPIKNFF+FD  DGKG+IEDIS+ +
Sbjct:  147 GHVSSADLESVLILQKKLCEEQGLSESRIPDILLERILDAGTRQLPPVCAIIGGILGQEVIKAMSCKGDPIKNFFFFDVMDGKGIIEDISNQD 239

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1A_ARATH (SUMO-activating enzyme subunit 1A OS=Arabidopsis thaliana GN=SAE1A PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 5.959e-33
Identity = 63/91 (69.23%), Postives = 78/91 (85.71%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDS 279
            G+ S  DL+ VL++KK+LC A S++ESH+PD LLERL+  T EFPPVCA++GGIL QEVIKA+SGKGDP+KNFFY+D  DGKGV+EDISDS
Sbjct:  229 GECSLLDLARVLEIKKQLCEANSVSESHIPDILLERLITGTTEFPPVCAIVGGILAQEVIKAVSGKGDPLKNFFYYDGEDGKGVMEDISDS 319

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1B_ARATH (SUMO-activating enzyme subunit 1B OS=Arabidopsis thaliana GN=SAE1B PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.264e-32
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  229 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 317

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_DANRE (SUMO-activating enzyme subunit 1 OS=Danio rerio GN=sae1 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.182e-10
Identity = 29/79 (36.71%), Postives = 50/79 (63.29%), Query Frame = 1

Query:   37 VLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            +L+++ ++     L+   +P+T +    +   E  PVCAV+GG+LGQE++KA+S +  P +NFF+FD   G GV++  S
Sbjct:  271 LLQIRDDVLETMGLSSDLLPNTFVSYCFS---EMSPVCAVVGGVLGQEIVKALSQRDAPHRNFFFFDGLKGSGVVDYFS 346

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_PONAB (SUMO-activating enzyme subunit 1 OS=Pongo abelii GN=SAE1 PE=2 SV=2)

HSP 1 Score: 63.929 bits (154), Expect = 5.427e-10
Identity = 29/87 (33.33%), Postives = 52/87 (59.77%), Query Frame = 1

Query:    4 AGDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            + D  E D   +L+++ ++  +  ++   +P+  +    ++ A   PVCAV+GGIL QE++KA+S +  P  NFF+FD   G G++E
Sbjct:  258 SSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA---PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVE 341

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_HUMAN (SUMO-activating enzyme subunit 1 OS=Homo sapiens GN=SAE1 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.427e-10
Identity = 29/87 (33.33%), Postives = 52/87 (59.77%), Query Frame = 1

Query:    4 AGDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            + D  E D   +L+++ ++  +  ++   +P+  +    ++ A   PVCAV+GGIL QE++KA+S +  P  NFF+FD   G G++E
Sbjct:  258 SSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA---PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVE 341

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_BOVIN (SUMO-activating enzyme subunit 1 OS=Bos taurus GN=SAE1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.427e-10
Identity = 30/87 (34.48%), Postives = 51/87 (58.62%), Query Frame = 1

Query:    4 AGDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            + D    D   +L+++ ++  A  +N   +P+  +    ++ A   PVCAV+GGIL QE++KA+S +  P  NFF+FD   G G++E
Sbjct:  258 SSDTFGEDSELLLQIRNDVLDALGVNPDLLPEDFVRYCFSEMA---PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVE 341

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_RAT (SUMO-activating enzyme subunit 1 OS=Rattus norvegicus GN=Sae1 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.062e-9
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1

Query:   10 DVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            D    D   +L+++ ++  +  ++   +PD  +    ++ A   PVCAV+GGIL QE++KA+S +  P  NFF+FD   G G++E
Sbjct:  263 DSYSEDAELLLQIRNDVFDSLGVSPDLLPDDFVRYCFSEMA---PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVE 344

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_MOUSE (SUMO-activating enzyme subunit 1 OS=Mus musculus GN=Sae1 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.062e-9
Identity = 28/80 (35.00%), Postives = 49/80 (61.25%), Query Frame = 1

Query:   25 DLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            D   +L+++ ++  +  ++   +PD  +    ++ A   PVCAV+GGIL QE++KA+S +  P  NFF+FD   G G++E
Sbjct:  269 DAELLLQIRNDVFDSLGISPDLLPDDFVRYCFSEMA---PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVE 345

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_XENTR (SUMO-activating enzyme subunit 1 OS=Xenopus tropicalis GN=sae1 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 2.978e-8
Identity = 22/45 (48.89%), Postives = 33/45 (73.33%), Query Frame = 1

Query:  130 AEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            +E  PVCAV+GG+LGQE++KA+S +  P  NFF+FD     G+++
Sbjct:  298 SEMAPVCAVVGGVLGQEIVKALSQRDAPHNNFFFFDGRSSNGIVD 342

[Close]

BLAST of FG532994 vs. SwissProt
Match: SAE1_XENLA (SUMO-activating enzyme subunit 1 OS=Xenopus laevis GN=sae1 PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.080e-8
Identity = 22/45 (48.89%), Postives = 33/45 (73.33%), Query Frame = 1

Query:  130 AEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIE 264
            +E  PVCAV+GG+LGQE++KA+S +  P  NFF+FD     G+++
Sbjct:  295 SEMAPVCAVVGGVLGQEIVKALSLRDAPHNNFFFFDGKTSNGIVD 339

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT4G24940.1 (| Symbols: SAE1A, AT-SAE1-1, ATSAE1A | SUMO-activating enzyme 1A | chr4:12823651-12825971 FORWARD LENGTH=322)

HSP 1 Score: 140.198 bits (352), Expect = 6.511e-34
Identity = 63/91 (69.23%), Postives = 78/91 (85.71%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDISDS 279
            G+ S  DL+ VL++KK+LC A S++ESH+PD LLERL+  T EFPPVCA++GGIL QEVIKA+SGKGDP+KNFFY+D  DGKGV+EDISDS
Sbjct:  229 GECSLLDLARVLEIKKQLCEANSVSESHIPDILLERLITGTTEFPPVCAIVGGILAQEVIKAVSGKGDPLKNFFYYDGEDGKGVMEDISDS 319

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT5G50680.2 (| Symbols: ATSAE1B, SAE1B | SUMO activating enzyme 1B | chr5:20618842-20620743 FORWARD LENGTH=318)

HSP 1 Score: 138.272 bits (347), Expect = 2.474e-33
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  227 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 315

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT5G50580.2 (| Symbols: SAE1B, AT-SAE1-2 | SUMO-activating enzyme 1B | chr5:20585500-20587401 FORWARD LENGTH=320)

HSP 1 Score: 138.272 bits (347), Expect = 2.474e-33
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  229 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 317

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT5G50680.1 (| Symbols: ATSAE1B, SAE1B | SUMO activating enzyme 1B | chr5:20618842-20620743 FORWARD LENGTH=320)

HSP 1 Score: 138.272 bits (347), Expect = 2.474e-33
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  229 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 317

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT5G50580.1 (| Symbols: SAE1B, AT-SAE1-2 | SUMO-activating enzyme 1B | chr5:20585500-20587401 FORWARD LENGTH=318)

HSP 1 Score: 138.272 bits (347), Expect = 2.474e-33
Identity = 64/89 (71.91%), Postives = 76/89 (85.39%), Query Frame = 1

Query:    7 GDVSEADLSDVLKLKKELCTAQSLNESHVPDTLLERLVADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDASDGKGVIEDIS 273
            G+ S +DL  VLKLKKELC   S++E+H+PD LLERLV++  EFPP CA+IGGILGQEVIK ISGKG+P+KNFFYFDA DGKGVIED+S
Sbjct:  227 GECSLSDLPRVLKLKKELCEGNSVSENHIPDILLERLVSNNTEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFDAEDGKGVIEDLS 315

[Close]

BLAST of FG532994 vs. TAIR peptide
Match: AT2G30110.1 (| Symbols: ATUBA1, MOS5, UBA1 | ubiquitin-activating enzyme 1 | chr2:12852632-12857369 REVERSE LENGTH=1080)

HSP 1 Score: 50.0618 bits (118), Expect = 8.861e-7
Identity = 30/77 (38.96%), Postives = 41/77 (53.25%), Query Frame = 1

Query:   16 SEADLSDVLKLKKELCTAQS-LNESHVPDTLLERL-VADTAEFPPVCAVIGGILGQEVIKAISGKGDPIKNFFYFDA 240
            SE D   ++ +   + T Q  L   +V   LL        A   P+ A+ GGI+GQEV+KA SGK  P+  FFYFD+
Sbjct:  378 SEEDAQKLISIATAINTGQGDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDS 454

The following BLAST results are available for this feature:

BLAST of FG532994 vs. TrEMBL
BLAST of FG532994 vs. SwissProt
BLAST of FG532994 vs. TAIR peptide

BLAST of FG532994 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SGZ8_VITVI	2.130e-37	79.79	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BME4_VITVI	2.604e-35	74.26	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C6TF08_SOYBN	4.911e-34	78.65	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9IFE2_POPTR	6.415e-34	76.09	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I3U4_POPTR	4.158e-33	76.09	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q0WNE6_ARATH	2.980e-31	71.91	Ubiquitin activating enzyme OS=Arabidopsis thalian...	[more]
D7MQ99_ARALY	2.980e-31	71.11	Sumo activating enzyme 1b OS=Arabidopsis lyrata su...	[more]
B9RAD1_RICCO	1.132e-30	73.86	DNA damage tolerance protein rad31, putative OS=Ri...	[more]
D7MGL4_ARALY	3.294e-30	65.93	Sumo-activating enzyme 1A OS=Arabidopsis lyrata su...	[more]
O65078_PICMA	1.530e-27	66.67	Probable ubiquitin activating enzyme 2 (Fragment) ...	[more]

BLAST of FG532994 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SAE1A_ARATH	5.959e-33	69.23	SUMO-activating enzyme subunit 1A OS=Arabidopsis t...	[more]
SAE1B_ARATH	2.264e-32	71.91	SUMO-activating enzyme subunit 1B OS=Arabidopsis t...	[more]
SAE1_DANRE	3.182e-10	36.71	SUMO-activating enzyme subunit 1 OS=Danio rerio GN...	[more]
SAE1_PONAB	5.427e-10	33.33	SUMO-activating enzyme subunit 1 OS=Pongo abelii G...	[more]
SAE1_HUMAN	5.427e-10	33.33	SUMO-activating enzyme subunit 1 OS=Homo sapiens G...	[more]
SAE1_BOVIN	5.427e-10	34.48	SUMO-activating enzyme subunit 1 OS=Bos taurus GN=...	[more]
SAE1_RAT	2.062e-9	34.12	SUMO-activating enzyme subunit 1 OS=Rattus norvegi...	[more]
SAE1_MOUSE	2.062e-9	35.00	SUMO-activating enzyme subunit 1 OS=Mus musculus G...	[more]
SAE1_XENTR	2.978e-8	48.89	SUMO-activating enzyme subunit 1 OS=Xenopus tropic...	[more]
SAE1_XENLA	5.080e-8	48.89	SUMO-activating enzyme subunit 1 OS=Xenopus laevis...	[more]

BLAST of FG532994 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT4G24940.1	6.511e-34	69.23	\| Symbols: SAE1A, AT-SAE1-1, ATSAE1A \| SUMO-activa...	[more]
AT5G50680.2	2.474e-33	71.91	\| Symbols: ATSAE1B, SAE1B \| SUMO activating enzyme...	[more]
AT5G50580.2	2.474e-33	71.91	\| Symbols: SAE1B, AT-SAE1-2 \| SUMO-activating enzy...	[more]
AT5G50680.1	2.474e-33	71.91	\| Symbols: ATSAE1B, SAE1B \| SUMO activating enzyme...	[more]
AT5G50580.1	2.474e-33	71.91	\| Symbols: SAE1B, AT-SAE1-2 \| SUMO-activating enzy...	[more]
AT2G30110.1	8.861e-7	38.96	\| Symbols: ATUBA1, MOS5, UBA1 \| ubiquitin-activati...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009036	Molybdenum cofactor biosynthesis, MoeB	SUPERFAMILY	69572	Activating enzymes of the ubiquitin-like proteins	coord: 24..90 score: 3.1
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 21..85 score: 8.6
None	No IPR available	PANTHER	PTHR10953	UBIQUITIN-ACTIVATING ENZYME E1	coord: 3..56 score: 3.5
None	No IPR available	PANTHER	PTHR10953:SF10	UBIQUITIN ACTIVATING ENZYME - RELATED	coord: 3..56 score: 3.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG532994 ID=FG532994; Name=FG532994; organism=Pisum sativum; type=EST; length=536bp
GGTGCAGGAGATGTTTCGGAGGCAGATCTTTCCGATGTTCTCAAGCTTAA
AAAGGAACTTTGTACTGCACAATCTCTCAATGAATCTCATGTTCCCGACA
CTCTTCTAGAAAGATTGGTAGCGGATACAGCCGAGTTTCCTCCAGTCTGT
GCTGTTATTGGGGGAATCCTTGGACAGGAGGTTATCAAAGCTATTTCTGG
AAAAGGAGACCCCATAAAAAACTTCTTCTATTTTGACGCTTCTGATGGAA
AGGGTGTTATAGAAGACATATCTGATTCAAACGCTGGAAAGTAGCTTCTC
TGGGTCAGTGTTTCTAATGAGTCGCCCGCAATCGTGGTTGTGAATATTGA
ACCTTTTGCTGCATTAACTGCTCATTTATAGTTTAGTAGTTCCTTATTTG
TTTTAGTCATTGGCAATTTAGGATCAGAAATGTTTTGAAATGTAGACACT
CGAATGTATTTATGCTGACCTTCAAAGCCATCTTCGAAGTTGCAAAGCTT
CATGATGGTTAGGAAAAAAGCTCAACGTGTTTATAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR009036	Molybdenum cofactor biosynthesis, MoeB
IPR016040	NAD(P)-bd_dom

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