FG535128

EST Overview


Name	FG535128
Unique Name	FG535128
Type	EST
Organism	Pisum sativum (pea)
Sequence length	586

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG535128 vs. TrEMBL
Match: Q0GXX6_MEDTR (Auxin conjugate hydrolase OS=Medicago truncatula GN=IAR32 PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 4.199e-62
Identity = 117/137 (85.40%), Postives = 126/137 (91.97%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            EQIITGQAAVQRC+ATVSFLEE KP  PPTVN+  LH YFQSVA SLLGADKVKG++PMMGSEDFAFYQEA+PGYIFLLGMEDVSVE LPSGHSP+FKVNEDVLPYGAALHASLA +YL KL QE+P+VEGKYHDEL
Sbjct:  311 EQIITGQAAVQRCHATVSFLEEEKPFFPPTVNDGGLHDYFQSVAGSLLGADKVKGMQPMMGSEDFAFYQEAIPGYIFLLGMEDVSVERLPSGHSPYFKVNEDVLPYGAALHASLASRYLLKLRQEVPIVEGKYHDEL 447

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BLAST of FG535128 vs. TrEMBL
Match: Q0GXX7_MEDTR (Auxin conjugate hydrolase OS=Medicago truncatula GN=IAR31 PE=2 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 3.114e-57
Identity = 107/137 (78.10%), Postives = 119/137 (86.86%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            EQ+ITGQAAV RCNATV+FLEE  P IPPTVNN  LH YF+SVA  LLG DK+K  +P +GSEDFAFYQEA+PGYIFLLGMEDVS+E LPSGHSP+FKVNED LPYGAALHASLA +YL KLHQE+PVV+GK HDEL
Sbjct:  316 EQVITGQAAVHRCNATVNFLEEETPFIPPTVNNGGLHDYFESVAGRLLGVDKIKDQQPTVGSEDFAFYQEAIPGYIFLLGMEDVSIERLPSGHSPYFKVNEDALPYGAALHASLASRYLVKLHQEVPVVKGKIHDEL 452

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BLAST of FG535128 vs. TrEMBL
Match: B9RQ74_RICCO (IAA-amino acid hydrolase ILR1, putative OS=Ricinus communis GN=RCOM_1486000 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 9.399e-46
Identity = 87/137 (63.50%), Postives = 109/137 (79.56%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++ITGQA+VQRC ATV FLE+ KP  PPTVN+  LH +F +VA  +LG+DKVK ++P+MGSEDFAFYQE +PGYIF +GM++ + + L S HSPHF++NEDVLPYGAALHASLA +YL  L  E P+   KYHDEL
Sbjct:  299 EEVITGQASVQRCKATVDFLEKDKPPFPPTVNDKKLHEFFATVAGDVLGSDKVKDMQPLMGSEDFAFYQEIMPGYIFFIGMQNETRKKLQSAHSPHFEINEDVLPYGAALHASLATRYLLNLQPEHPLPVEKYHDEL 435

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BLAST of FG535128 vs. TrEMBL
Match: D7U044_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016901001 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.949e-44
Identity = 84/137 (61.31%), Postives = 106/137 (77.37%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++IT QAAVQRCNATV F E+ KPL P T+NN +LH +FQ+V  ++LG   VK ++P+MGSEDF+FYQE +PGY F LGM+D ++  LPS HSPHFK+NED LPYGAALHASLA  YL ++  +    EGK+HDEL
Sbjct:  305 EEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVVGNMLGVHNVKDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEDALPYGAALHASLAATYLLEIQPQPSSREGKHHDEL 441

[Close]

BLAST of FG535128 vs. TrEMBL
Match: A5BUS2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033718 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 8.797e-44
Identity = 84/137 (61.31%), Postives = 106/137 (77.37%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++IT QAAVQRCNATV F E+ KPL P T+NN +LH +FQ+VA ++LG   VK ++P+MGSEDF+FYQE +PGY F LGM+D ++  LPS HSPHFK+NE  LPYGAALHASLA  YL ++  +    EGK+HDEL
Sbjct:  305 EEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVAGNMLGVHNVKDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEGALPYGAALHASLAATYLLEIQPQPSSREGKHHDEL 441

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BLAST of FG535128 vs. TrEMBL
Match: B9GVN2_POPTR (Iaa-amino acid hydrolase 11 OS=Populus trichocarpa GN=ILL11 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 9.726e-43
Identity = 80/137 (58.39%), Postives = 105/137 (76.64%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E+++TGQAAVQRC A ++FLE  KP  PPT+N+  LH YF+ VA  +LG DKVK ++P+MGSEDFAFYQE +PGY F +GM++ + + L S HSP+F++NEDVLPYGAALHASLA +YL +   E+ + E   HDEL
Sbjct:  302 EEVVTGQAAVQRCKAVINFLENEKPFFPPTINDKYLHDYFRIVASDMLGIDKVKDMQPLMGSEDFAFYQEMIPGYFFFIGMQNETHKQLQSPHSPYFEINEDVLPYGAALHASLAARYLLEFQPEVTLPEENDHDEL 438

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BLAST of FG535128 vs. TrEMBL
Match: D5FTH2_POPTO (IAA-amino acid hydrolase OS=Populus tomentosa GN=IAR3 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.270e-42
Identity = 79/137 (57.66%), Postives = 105/137 (76.64%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E+++TGQAAVQRC A ++FLE  KP  PPT+N+ +LH YF+ VA  +LG DKVK ++P+MGSEDFAFYQE +PGY F LGM++ + + L S HSP+F++NEDVLPYGAALH SLA +YL +   ++ + E   HDEL
Sbjct:  302 EEVVTGQAAVQRCKAVINFLENEKPFFPPTINDKNLHDYFRVVASDVLGTDKVKDMQPLMGSEDFAFYQEKIPGYFFFLGMQNETRKQLQSPHSPYFEINEDVLPYGAALHVSLAARYLLEFQPQVTLPEENDHDEL 438

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BLAST of FG535128 vs. TrEMBL
Match: Q6H8S2_9ROSI (Putative auxin-amidohydrolase OS=Populus tremula x Populus alba GN=Iar3 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 6.304e-42
Identity = 78/137 (56.93%), Postives = 105/137 (76.64%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E+++TGQAAVQRC A ++ LE  KP  PPT+N+ +LH YF+ VA  +LG DKVK ++P+MGSEDFAFYQE +PGY F +GM++ + + L S HSP+F++NEDVLPYGAALHASLA +YL +   ++ + E   HDEL
Sbjct:  302 EEVVTGQAAVQRCKAVINLLENEKPFFPPTINDKNLHDYFRVVASDVLGIDKVKDMQPLMGSEDFAFYQEKIPGYFFFVGMQNETRKQLQSPHSPYFEINEDVLPYGAALHASLAARYLLEFQPQVTLPEENDHDEL 438

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BLAST of FG535128 vs. TrEMBL
Match: A8VJB6_EUCUL (IAA-amino acid hydrolase 3 (Fragment) OS=Eucommia ulmoides GN=IAR3 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 4.518e-40
Identity = 79/137 (57.66%), Postives = 99/137 (72.26%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++I  Q+AVQRCNATV FL E KP  PPTVN+ DLHH+FQ VA  +LG   VK +EP+MGSEDF+FYQE +PGY F LG+     E   S HSP+F +NED LP+GA+LHASLA KYL +  +E P +  ++H EL
Sbjct:  141 EEVIIRQSAVQRCNATVDFLTEDKPFFPPTVNHQDLHHHFQKVAGEMLGHHNVKHMEPLMGSEDFSFYQERIPGYFFFLGVRPEGHEKPASVHSPYFTINEDSLPFGASLHASLAYKYLVEFRKETPTLPTQHHGEL 277

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BLAST of FG535128 vs. TrEMBL
Match: Q0GXX4_MEDTR (Auxin conjugate hydrolase OS=Medicago truncatula GN=IAR34 PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 6.524e-39
Identity = 86/141 (60.99%), Postives = 104/141 (73.76%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKG-LEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPS-GHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQE--LPVVEGKYHDEL 411
            E++I GQAAV RCNATV FL  VKP  PPTVNN DLH +F +VA ++LG DKV   + P MGSEDF+FYQE +PGY F+LG+++ S +   S  HSP+ ++NED LPYGAALHASLA  YL K HQ   +P VE KYHDEL
Sbjct:  308 EEVIIGQAAVHRCNATVDFLHGVKPFYPPTVNNADLHEHFVNVAVNMLGIDKVDSVMTPYMGSEDFSFYQEVIPGYFFMLGVKNASHKRFESLLHSPYLEINEDGLPYGAALHASLAASYLLK-HQRDTVPGVERKYHDEL 447

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BLAST of FG535128 vs. SwissProt
Match: ILL4_ARATH (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana GN=ILL4 PE=1 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 2.279e-34
Identity = 64/122 (52.46%), Postives = 94/122 (77.05%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKL 366
            EQ+IT QA+V  CNATV F+EE KP  PPTVN+  LH +F++V+  +LG +    ++P+MGSEDF+FYQ+A+PG+   +GM++ +   + S HSP+F+VNE++LPYGA+LHAS+A +YL +L
Sbjct:  305 EQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYLLEL 426

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BLAST of FG535128 vs. SwissProt
Match: ILL1_ORYSJ (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 1.383e-31
Identity = 67/138 (48.55%), Postives = 92/138 (66.67%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPG-YIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++I  QA+VQRCNA V FL++ +P  PPT+N+  LH +F  VA  ++G   V+  +P+MG+EDFAFY +A+P  Y + LGM + +       HSP+F +NED LPYGAAL ASLA +YL + HQ     + K HDEL
Sbjct:  306 EEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHHSPYFTINEDALPYGAALQASLAARYLLE-HQPPTTGKAKAHDEL 442

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BLAST of FG535128 vs. SwissProt
Match: ILL1_ORYSI (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 1.383e-31
Identity = 67/138 (48.55%), Postives = 92/138 (66.67%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPG-YIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            E++I  QA+VQRCNA V FL++ +P  PPT+N+  LH +F  VA  ++G   V+  +P+MG+EDFAFY +A+P  Y + LGM + +       HSP+F +NED LPYGAAL ASLA +YL + HQ     + K HDEL
Sbjct:  306 EEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHHSPYFTINEDALPYGAALQASLATRYLLE-HQPPTTGKAKAHDEL 442

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BLAST of FG535128 vs. SwissProt
Match: ILL1_ARATH (IAA-amino acid hydrolase ILR1-like 1 OS=Arabidopsis thaliana GN=ILL1 PE=1 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 1.690e-29
Identity = 66/137 (48.18%), Postives = 91/137 (66.42%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            ++IIT QAAV RCNA+V+        +PPTVNN DL+  F+ V + LLG +      P MGSEDF+++ E +PG+  LLGM+D   +   S HSPH+++NEDVLPYGAA+HA++AV+YL     +  V    +HDEL
Sbjct:  305 KEIITKQAAVHRCNASVNLAPNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQD-ETQGYASSHSPHYRINEDVLPYGAAIHATMAVQYLKDKASKGSV--SGFHDEL 438

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BLAST of FG535128 vs. SwissProt
Match: ILL5_ARATH (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana GN=ILL5 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 4.917e-29
Identity = 61/118 (51.69%), Postives = 85/118 (72.03%), Query Frame = 1

Query:    4 QIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYL 357
            Q+IT QA+V  CNATV FLE+  P  PPTVNN  LH ++++V+  +LG +      P+M SEDFAFYQ+A+PG+   +GM++ S   + + HSP F+VNE++LPYGA+L ASLA +YL
Sbjct:  306 QVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYL 423

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BLAST of FG535128 vs. SwissProt
Match: ILL2_ARATH (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana GN=ILL2 PE=1 SV=2)

HSP 1 Score: 127.487 bits (319), Expect = 4.917e-29
Identity = 64/137 (46.72%), Postives = 92/137 (67.15%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            +++IT QAAV RCNA+V+     +  +PPTVNN DL+  F+ V + LLG +      P+MGSEDF+++ E +PG+  LLGM+D       S HSP +++NEDVLPYGAA+HAS+AV+YL +   +  V    +H+EL
Sbjct:  306 KEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQD-ETNGYASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKASKGSV--SGFHEEL 439

[Close]

BLAST of FG535128 vs. SwissProt
Match: ILL2_ORYSJ (IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica GN=ILL2 PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.481e-25
Identity = 59/128 (46.09%), Postives = 86/128 (67.19%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADK--VKGLEPMMGSEDFAFYQEALP-GYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQE 375
            E++I  Q+AV RC A V F    +PL+PPT+N+  LH +FQ+VA   LGA    +  +EP MGSEDFA + EA+P  + + +G+ + +   +   HSPHF+V++  LPYGAALHASLA++YL +  +E
Sbjct:  320 EEVIVAQSAVYRCAAAVDFHAGGRPLLPPTINSAALHAHFQAVAAETLGASAAVLGAMEPCMGSEDFAVFSEAVPASHFYFVGVRNEAEGLVHLAHSPHFRVDDAALPYGAALHASLAMRYLDERRRE 447

[Close]

BLAST of FG535128 vs. SwissProt
Match: ILL5_ORYSJ (IAA-amino acid hydrolase ILR1-like 5 OS=Oryza sativa subsp. japonica GN=ILL5 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 7.348e-25
Identity = 54/125 (43.20%), Postives = 80/125 (64.00%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQE 375
            ++++ GQAAV RC   V    +  P+ P   N++ LHH+ ++V + LLG DKVK  E +M  EDFAFYQ+ +PG +F +G+ +  V  + + H+P F V+EDV+P GAALH +LA  YL +   E
Sbjct:  293 KEVVEGQAAVHRCKGVVQIKRDDYPMYPAVFNDEKLHHHVETVGRRLLGPDKVKPGEKIMAGEDFAFYQQLVPGVMFGIGIRNGEVGSVHTVHNPKFFVDEDVIPIGAALHTALAEMYLTERSTE 417

[Close]

BLAST of FG535128 vs. SwissProt
Match: ILL7_ORYSJ (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica GN=ILL7 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.637e-24
Identity = 56/122 (45.90%), Postives = 82/122 (67.21%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGM-EDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAK 363
            ++I+   A V RC ATV F+EE +   P TVN++ ++ + ++VA  +LG D VK   P MGSEDFAFY +  P   F++G+  + ++  +   HSPHF V+EDVLP GAALHA++A++YL K
Sbjct:  327 KEIVEAHATVHRCTATVDFMEEERIPYPATVNDEGMYRHARAVAVDVLGEDGVKVGTPFMGSEDFAFYAQRFPAAFFMIGVGNETTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYLNK 448

[Close]

BLAST of FG535128 vs. SwissProt
Match: ILL6_ORYSJ (IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sativa subsp. japonica GN=ILL6 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.647e-24
Identity = 52/120 (43.33%), Postives = 81/120 (67.50%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLA 360
            E++IT QA V  C A V F E  +   PPTVN+  ++ + ++VA  LLGA   + + PMMG+EDF+FY + +P   + +G+ + ++  + +GHSP+F ++EDVLP GAA HA++A +YLA
Sbjct:  370 EEVITAQARVHGCEAAVDFFEN-QSFYPPTVNDARMYAHVKAVAGELLGAGSYRDVPPMMGAEDFSFYSQVVPAGFYYIGVRNETLGSVHTGHSPYFMIDEDVLPTGAAFHAAIAERYLA 488

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT1G51760.1 (| Symbols: IAR3, JR3 | peptidase M20/M25/M40 family protein | chr1:19199562-19201424 FORWARD LENGTH=440)

HSP 1 Score: 145.206 bits (365), Expect = 2.402e-35
Identity = 64/122 (52.46%), Postives = 94/122 (77.05%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKL 366
            EQ+IT QA+V  CNATV F+EE KP  PPTVN+  LH +F++V+  +LG +    ++P+MGSEDF+FYQ+A+PG+   +GM++ +   + S HSP+F+VNE++LPYGA+LHAS+A +YL +L
Sbjct:  305 EQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYLLEL 426

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT5G56650.1 (| Symbols: ILL1 | IAA-leucine resistant (ILR)-like 1 | chr5:22930825-22932488 FORWARD LENGTH=438)

HSP 1 Score: 129.028 bits (323), Expect = 1.781e-30
Identity = 66/137 (48.18%), Postives = 91/137 (66.42%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            ++IIT QAAV RCNA+V+        +PPTVNN DL+  F+ V + LLG +      P MGSEDF+++ E +PG+  LLGM+D   +   S HSPH+++NEDVLPYGAA+HA++AV+YL     +  V    +HDEL
Sbjct:  305 KEIITKQAAVHRCNASVNLAPNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQD-ETQGYASSHSPHYRINEDVLPYGAAIHATMAVQYLKDKASKGSV--SGFHDEL 438

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT5G56660.1 (| Symbols: ILL2 | IAA-leucine resistant (ILR)-like 2 | chr5:22933278-22935011 FORWARD LENGTH=439)

HSP 1 Score: 127.487 bits (319), Expect = 5.182e-30
Identity = 64/137 (46.72%), Postives = 92/137 (67.15%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQELPVVEGKYHDEL 411
            +++IT QAAV RCNA+V+     +  +PPTVNN DL+  F+ V + LLG +      P+MGSEDF+++ E +PG+  LLGM+D       S HSP +++NEDVLPYGAA+HAS+AV+YL +   +  V    +H+EL
Sbjct:  306 KEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQD-ETNGYASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKASKGSV--SGFHEEL 439

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT1G51780.1 (| Symbols: ILL5 | IAA-leucine resistant (ILR)-like gene 5 | chr1:19204602-19206453 FORWARD LENGTH=435)

HSP 1 Score: 127.487 bits (319), Expect = 5.182e-30
Identity = 61/118 (51.69%), Postives = 85/118 (72.03%), Query Frame = 1

Query:    4 QIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYL 357
            Q+IT QA+V  CNATV FLE+  P  PPTVNN  LH ++++V+  +LG +      P+M SEDFAFYQ+A+PG+   +GM++ S   + + HSP F+VNE++LPYGA+L ASLA +YL
Sbjct:  306 QVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYL 423

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT5G54140.1 (| Symbols: ILL3 | IAA-leucine-resistant (ILR1)-like 3 | chr5:21965833-21967834 FORWARD LENGTH=428)

HSP 1 Score: 109.768 bits (273), Expect = 1.118e-24
Identity = 54/124 (43.55%), Postives = 80/124 (64.52%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLHQ 372
            ++++ GQA VQRC A +   E+  P+ P TVN+  LH + + V K LLG +KVK    +M  EDFAFYQ+ +PGY   +G+ +  +  + S HSP+F ++E+VLP G+A  A+LA  YL + HQ
Sbjct:  299 KEVVEGQAEVQRCKADIDMHEDDHPMYPATVNDHKLHEFTEKVLKLLLGPEKVKPANKVMAGEDFAFYQQKIPGYYIGIGIRNEEIGSVRSVHSPYFFLDENVLPIGSATFAALAEMYLQE-HQ 421

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT1G44350.1 (| Symbols: ILL6 | IAA-leucine resistant (ILR)-like gene 6 | chr1:16834749-16838201 REVERSE LENGTH=464)

HSP 1 Score: 103.605 bits (257), Expect = 8.014e-23
Identity = 48/123 (39.02%), Postives = 73/123 (59.35%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDDLHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYLAKLH 369
            ++++  Q  V  C ATV+F E+   + PPT NND  +++ + V   LLG         MMG+EDFAFY E +P   + +G+ +  +  +   HSPHF ++ED LP GAA+HA++A +YL   H
Sbjct:  341 QEVLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKH 463

[Close]

BLAST of FG535128 vs. TAIR peptide
Match: AT3G02875.1 (| Symbols: ILR1 | Peptidase M20/M25/M40 family protein | chr3:631993-633859 FORWARD LENGTH=442)

HSP 1 Score: 92.4337 bits (228), Expect = 1.847e-19
Identity = 49/120 (40.83%), Postives = 78/120 (65.00%), Query Frame = 1

Query:    1 EQIITGQAAVQRCNATVSFLEEVKPLIPPTVNNDD-LHHYFQSVAKSLLGADKVKGLEPMMGSEDFAFYQEALPGYIFLLGMEDVSVEHLPSGHSPHFKVNEDVLPYGAALHASLAVKYL 357
            ++I   QA+V RC A V+F EE KP + P +NND+ L+ + + VA++++G +        MG EDF+F+ +     IF+LG+++ ++      HSP+F V+E+ LP GAALHA++AV YL
Sbjct:  309 KEISEAQASVYRCKAEVNF-EEKKPSLHPVMNNDEGLYEHGKKVAEAMIGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAVSYL 427

The following BLAST results are available for this feature:

BLAST of FG535128 vs. TrEMBL
BLAST of FG535128 vs. SwissProt
BLAST of FG535128 vs. TAIR peptide

BLAST of FG535128 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0GXX6_MEDTR	4.199e-62	85.40	Auxin conjugate hydrolase OS=Medicago truncatula G...	[more]
Q0GXX7_MEDTR	3.114e-57	78.10	Auxin conjugate hydrolase OS=Medicago truncatula G...	[more]
B9RQ74_RICCO	9.399e-46	63.50	IAA-amino acid hydrolase ILR1, putative OS=Ricinus...	[more]
D7U044_VITVI	3.949e-44	61.31	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BUS2_VITVI	8.797e-44	61.31	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9GVN2_POPTR	9.726e-43	58.39	Iaa-amino acid hydrolase 11 OS=Populus trichocarpa...	[more]
D5FTH2_POPTO	1.270e-42	57.66	IAA-amino acid hydrolase OS=Populus tomentosa GN=I...	[more]
Q6H8S2_9ROSI	6.304e-42	56.93	Putative auxin-amidohydrolase OS=Populus tremula x...	[more]
A8VJB6_EUCUL	4.518e-40	57.66	IAA-amino acid hydrolase 3 (Fragment) OS=Eucommia ...	[more]
Q0GXX4_MEDTR	6.524e-39	60.99	Auxin conjugate hydrolase OS=Medicago truncatula G...	[more]

BLAST of FG535128 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ILL4_ARATH	2.279e-34	52.46	IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsi...	[more]
ILL1_ORYSJ	1.383e-31	48.55	IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sati...	[more]
ILL1_ORYSI	1.383e-31	48.55	IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sati...	[more]
ILL1_ARATH	1.690e-29	48.18	IAA-amino acid hydrolase ILR1-like 1 OS=Arabidopsi...	[more]
ILL5_ARATH	4.917e-29	51.69	IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsi...	[more]
ILL2_ARATH	4.917e-29	46.72	IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsi...	[more]
ILL2_ORYSJ	1.481e-25	46.09	IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sati...	[more]
ILL5_ORYSJ	7.348e-25	43.20	IAA-amino acid hydrolase ILR1-like 5 OS=Oryza sati...	[more]
ILL7_ORYSJ	1.637e-24	45.90	IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sati...	[more]
ILL6_ORYSJ	3.647e-24	43.33	IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sati...	[more]

BLAST of FG535128 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G51760.1	2.402e-35	52.46	\| Symbols: IAR3, JR3 \| peptidase M20/M25/M40 famil...	[more]
AT5G56650.1	1.781e-30	48.18	\| Symbols: ILL1 \| IAA-leucine resistant (ILR)-like...	[more]
AT5G56660.1	5.182e-30	46.72	\| Symbols: ILL2 \| IAA-leucine resistant (ILR)-like...	[more]
AT1G51780.1	5.182e-30	51.69	\| Symbols: ILL5 \| IAA-leucine resistant (ILR)-like...	[more]
AT5G54140.1	1.118e-24	43.55	\| Symbols: ILL3 \| IAA-leucine-resistant (ILR1)-lik...	[more]
AT1G44350.1	8.014e-23	39.02	\| Symbols: ILL6 \| IAA-leucine resistant (ILR)-like...	[more]
AT3G02875.1	1.847e-19	40.83	\| Symbols: ILR1 \| Peptidase M20/M25/M40 family pro...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 30..115 score: 3.1
None	No IPR available	GENE3D	3.40.630.10	no description	coord: 13..121 score: 8.5
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 30..119 score: 7.3

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG535128 ID=FG535128; Name=FG535128; organism=Pisum sativum; type=EST; length=586bp
GAGCAGATCATTACTGGACAAGCTGCTGTGCAGAGATGCAATGCAACAGT
CAGCTTCTTGGAAGAAGTGAAGCCTCTCATCCCTCCGACTGTAAACAATG
ATGACTTGCATCACTATTTTCAAAGTGTCGCCAAGAGTTTACTCGGCGCC
GATAAAGTTAAAGGCCTAGAACCAATGATGGGATCAGAAGACTTTGCGTT
CTATCAAGAGGCTTTGCCGGGTTACATCTTCCTCCTCGGAATGGAGGATG
TCTCAGTTGAACATCTTCCGTCCGGACACTCACCCCATTTCAAAGTCAAC
GAAGATGTGCTTCCTTATGGTGCTGCGCTTCATGCCTCATTAGCTGTGAA
GTATCTTGCCAAACTTCATCAAGAGTTACCTGTAGTAGAGGGGAAATATC
ATGATGAATTATAGGAAAGATTATTAGAAAAAATCATTTGGTTGGACACA
TTTGTAGATGAATTTTCCATTTAAATCATTAACTGCAGCGATCTAGTGTG
GCAAATCTTTAGCTAATAAGCCACTCAGCTGTTTTGAATTTCCACAACAA
TGTCTCCTCACGCTAATATCACAGATTGCAATTTAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR002933	Peptidase_M20

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