FG535263

EST Overview
NameFG535263
Unique NameFG535263
TypeEST
OrganismPisum sativum (pea)
Sequence length567
Libraries
Library NameType
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of FG535263 vs. TrEMBL
Match: Q9ZPE1_ARATH (Phosphatidylinositol 4-kinase OS=Arabidopsis thaliana GN=PI4K PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1059 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1121
[Close]
BLAST of FG535263 vs. TrEMBL
Match: Q9LLP5_ORYSA (Putative phosphatidylinositol 4-kinase OS=Oryza sativa GN=DUPR11.7 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1146 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSMDAWRTRQYDYYQRVLNGIL 1208
[Close]
BLAST of FG535263 vs. TrEMBL
Match: Q9FMJ0_ARATH (At5g64070/MHJ24_5 OS=Arabidopsis thaliana GN=At5g64070 PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1059 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1121
[Close]
BLAST of FG535263 vs. TrEMBL
Match: Q8LPD1_ORYSA (Phophatdylinositol 4-kinase (Fragment) OS=Oryza sativa GN=pi4k1 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct:  216 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSMDAWRTRQYDYYQRVLNGIL 278
[Close]
BLAST of FG535263 vs. TrEMBL
Match: D7U4E9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_44.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00026496001 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1065 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1127
[Close]
BLAST of FG535263 vs. TrEMBL
Match: D7MQK7_ARALY (Phosphatidylinositol 4-kinase (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496602 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1061 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1123
[Close]
BLAST of FG535263 vs. TrEMBL
Match: D7M228_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487746 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1056 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1118
[Close]
BLAST of FG535263 vs. TrEMBL
Match: C5Y1Y5_SORBI (Putative uncharacterized protein Sb05g009420 OS=Sorghum bicolor GN=Sb05g009420 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1140 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSMDAWRTRQYDYYQRVLNGIL 1202
[Close]
BLAST of FG535263 vs. TrEMBL
Match: B9V0T3_ORYRU (Phosphatidylinositol kinase OS=Oryza rufipogon GN=OR_CBa141L10-6 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1153 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSMDAWRTRQYDYYQRVLNGIL 1215
[Close]
BLAST of FG535263 vs. TrEMBL
Match: B9V0S2_ORYPU (Phosphatidylinositol kinase OS=Oryza punctata GN=OP_Ba004F03-6 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.358e-21
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1185 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSMDAWRTRQYDYYQRVLNGIL 1247
[Close]
BLAST of FG535263 vs. TAIR peptide
Match: AT5G64070.1 (| Symbols: PI-4KBETA1, PI4KBETA1 | phosphatidylinositol 4-OH kinase beta1 | chr5:25637492-25643902 REVERSE LENGTH=1121)

HSP 1 Score: 104.76 bits (260), Expect = 3.381e-23
Identity = 50/63 (79.37%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGIL 189
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGIL
Sbjct: 1059 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL 1121
[Close]
BLAST of FG535263 vs. TAIR peptide
Match: AT5G09350.1 (| Symbols: PI-4KBETA2, PI4KBETA2 | phosphatidylinositol 4-OH kinase beta2 | chr5:2899365-2905610 REVERSE LENGTH=1116)

HSP 1 Score: 103.219 bits (256), Expect = 9.838e-23
Identity = 49/62 (79.03%), Postives = 49/62 (79.03%), Query Frame = 1 
Query:    1 LQDSGFPCFKGGIRTIQNLRKRFHLSLTEEQCXXXXXXXXXXXXDAWRTRQYDYYQRVLNGI 186
            LQDSGFPCFKGG RTIQNLRKRFHLSLTEEQC            DAWRTRQYDYYQRVLNGI
Sbjct: 1054 LQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGI 1115
The following BLAST results are available for this feature:
BLAST of FG535263 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9ZPE1_ARATH4.358e-2179.37Phosphatidylinositol 4-kinase OS=Arabidopsis thali... [more]
Q9LLP5_ORYSA4.358e-2179.37Putative phosphatidylinositol 4-kinase OS=Oryza sa... [more]
Q9FMJ0_ARATH4.358e-2179.37At5g64070/MHJ24_5 OS=Arabidopsis thaliana GN=At5g6... [more]
Q8LPD1_ORYSA4.358e-2179.37Phophatdylinositol 4-kinase (Fragment) OS=Oryza sa... [more]
D7U4E9_VITVI4.358e-2179.37Whole genome shotgun sequence of line PN40024, sca... [more]
D7MQK7_ARALY4.358e-2179.37Phosphatidylinositol 4-kinase (Fragment) OS=Arabid... [more]
D7M228_ARALY4.358e-2179.37Putative uncharacterized protein OS=Arabidopsis ly... [more]
C5Y1Y5_SORBI4.358e-2179.37Putative uncharacterized protein Sb05g009420 OS=So... [more]
B9V0T3_ORYRU4.358e-2179.37Phosphatidylinositol kinase OS=Oryza rufipogon GN=... [more]
B9V0S2_ORYPU4.358e-2179.37Phosphatidylinositol kinase OS=Oryza punctata GN=O... [more]
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BLAST of FG535263 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G64070.13.381e-2379.37| Symbols: PI-4KBETA1, PI4KBETA1 | phosphatidylino... [more]
AT5G09350.19.838e-2379.03| Symbols: PI-4KBETA2, PI4KBETA2 | phosphatidylino... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000403Phosphatidylinositol 3-/4-kinase, catalyticGENE3D1.10.1070.11no descriptioncoord: 2..52
score: 4.1
IPR011009Protein kinase-like domainSUPERFAMILY56112Protein kinase-like (PK-like)coord: 1..63
score: 1.9
IPR015433Phosphatidylinositol KinasePANTHERPTHR10048PHOSPHATIDYLINOSITOL KINASEcoord: 1..63
score: 2.2
NoneNo IPR availablePANTHERPTHR10048:SF15PHOSPHATIDYLINOSITOL 4-KINASEcoord: 1..63
score: 2.2
NoneNo IPR availableSEGsegsegcoord: 33..44
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG535263 ID=FG535263; Name=FG535263; organism=Pisum sativum; type=EST; length=567bp
TTGCAGGATTCAGGGTTCCCATGCTTTAAAGGTGGTATACGGACGATACA
GAACTTACGAAAGCGATTCCATCTCAGTTTAACAGAAGAGCAATGTGTCT
CCTTGGTGCTTTCTCTAATTAGCAGTAGCCTCGATGCTTGGAGAACACGG
CAGTATGATTATTATCAGAGGGTTTTGAATGGAATATTGTGAGATAATTT
GTCATTGGTTATAATTTGGTTGTTCATTTGACCAAAGCTTGCCCGGTGGA
TGCTCATCAATTAGCATTACATTTAGAGGTCACTAGTGGGAGTGGTTGGT
TCACTATGGAATGGCTTTAAGTTTTGAGGGAGGAAAGGATTAATAGGTGG
TGGTATGCTGCCTCATTTTCTCCCGGTTCAAAGAAGACACTAACTTGCCA
AAAACTTCAACATAGACCATGTTAAATTTAATGAAGCCTGGTCTTTTTTA
TTTCCTCCTTCGCTCCATGTTAATGGAACTCTGCTGCTTGAAGAAGTTGC
ATTAGCCTCCTAAATGCCGATAATGCAGTGTACAGTGATTTTTAGTGTGA
CAGATCACATTTAAATT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004428obsolete inositol or phosphatidylinositol kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0046854phosphatidylinositol phosphate biosynthetic process
GO:0048015phosphatidylinositol-mediated signaling
Vocabulary: INTERPRO
TermDefinition
IPR000403PI3/4_kinase_cat_dom
IPR011009Kinase-like_dom_sf
IPR015433PI_Kinase