FG538273

EST Overview


Name	FG538273
Unique Name	FG538273
Type	EST
Organism	Pisum sativum (pea)
Sequence length	330

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q58FZ2_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana GN=At2g36540 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.195e-11
Identity = 35/61 (57.38%), Postives = 43/61 (70.49%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E N+D
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACEKNVD 117

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q8H1M2_ARATH (Putative uncharacterized protein F1O11.17 OS=Arabidopsis thaliana GN=F1O11.17 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.060e-10
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G LLHRVH ++ +  PK+ + D      L++KRP     MKFCLERFEVGIWSSA E N+D
Sbjct:   57 GLLLHRVHKKELRKKPKNXSPDASCGPNLVYKRPXAEEFMKFCLERFEVGIWSSACEKNVD 117

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q9SJQ4_ARATH (Putative uncharacterized protein At2g36540 OS=Arabidopsis thaliana GN=At2g36540 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.221e-10
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACE 113

[Close]

BLAST of FG538273 vs. TrEMBL
Match: D7LJ49_ARALY (NLI interacting factor family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482632 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.204e-9
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   56 GLLLHRVHKKEMRKKPKNRSPDASCGPNLVYKRPFSEEFMKFCLERFEVGIWSSACE 112

[Close]

BLAST of FG538273 vs. TrEMBL
Match: A9P944_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.594e-7
Identity = 28/61 (45.90%), Postives = 38/61 (62.30%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G L  RV  ++   IP +RT D     F ++KRPF    ++FCLERF+VGIWSSA   N++
Sbjct:   45 GVLCDRVFHKNRANIPDNRTPDAASGSFFVYKRPFCEEFVRFCLERFDVGIWSSARRTNLE 105

[Close]

BLAST of FG538273 vs. TrEMBL
Match: B9HVI4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_725378 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.542e-7
Identity = 30/58 (51.72%), Postives = 36/58 (62.07%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKG-IPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G L HRV +R   G +  +R  D  Y  F ++KRPF    +KFC ERFEVGIWSSA E
Sbjct:   57 GLLCHRVCIRRGSGNVQTNRRPDASYGSFKVYKRPFCDDFVKFCFERFEVGIWSSARE 114

[Close]

BLAST of FG538273 vs. TrEMBL
Match: A9PGJ7_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.542e-7
Identity = 30/58 (51.72%), Postives = 36/58 (62.07%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKG-IPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G L HRV +R   G +  +R  D  Y  F ++KRPF    +KFC ERFEVGIWSSA E
Sbjct:   57 GLLCHRVCIRRGSGNVQTNRRPDASYGSFKVYKRPFCDDFVKFCFERFEVGIWSSARE 114

[Close]

BLAST of FG538273 vs. TAIR peptide
Match: AT2G36540.1 (| Symbols: | Haloacid dehalogenase-like hydrolase (HAD) superfamily protein | chr2:15325237-15326449 FORWARD LENGTH=249)

HSP 1 Score: 66.6254 bits (161), Expect = 3.547e-12
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACE 113

[Close]

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACE 113

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q58FZ2_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana GN=At2g36540 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.745e-11
Identity = 35/61 (57.38%), Postives = 43/61 (70.49%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E N+D
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACEKNVD 117

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q8H1M2_ARATH (Putative uncharacterized protein F1O11.17 OS=Arabidopsis thaliana GN=F1O11.17 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.829e-10
Identity = 33/61 (54.10%), Postives = 41/61 (67.21%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G LLHRVH ++ +  PK+ + D      L++KRP     MKFCLERFEVGIWSSA E N+D
Sbjct:   57 GLLLHRVHKKELRKKPKNXSPDASCGPNLVYKRPXAEEFMKFCLERFEVGIWSSACEKNVD 117

[Close]

BLAST of FG538273 vs. TrEMBL
Match: Q9SJQ4_ARATH (Putative uncharacterized protein At2g36540 OS=Arabidopsis thaliana GN=At2g36540 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.153e-9
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   57 GLLLHRVHKKELRKKPKNRSPDASCGPNLVYKRPFAEEFMKFCLERFEVGIWSSACE 113

[Close]

BLAST of FG538273 vs. TrEMBL
Match: D7LJ49_ARALY (NLI interacting factor family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482632 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.506e-9
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query:   26 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME 196
            G LLHRVH ++ +  PK+R+ D      L++KRPF    MKFCLERFEVGIWSSA E
Sbjct:   56 GLLLHRVHKKEMRKKPKNRSPDASCGPNLVYKRPFSEEFMKFCLERFEVGIWSSACE 112

[Close]

BLAST of FG538273 vs. TrEMBL
Match: A9P944_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 6.996e-7
Identity = 28/61 (45.90%), Postives = 38/61 (62.30%), Query Frame = -3

Query:   14 GFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 196
            G L  RV  ++   IP +RT D     F ++KRPF    ++FCLERF+VGIWSSA   N++
Sbjct:   45 GVLCDRVFHKNRANIPDNRTPDAASGSFFVYKRPFCEEFVRFCLERFDVGIWSSARRTNLE 105

[Close]

BLAST of FG538273 vs. Lotus protein
Match: chr1.CM0378.310.r2.m (- phase: 1 /pseudo/partial)

HSP 1 Score: 84.7297 bits (208), Expect = 8.453e-18
Identity = 42/62 (67.74%), Postives = 47/62 (75.81%), Query Frame = -3

Query:    2 LHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNIDCQ*D 187
            L RV+VRD   IPKSRT D+KY   L++KRPF    MKFCLERFEVGIWSSAMEHN+D   D
Sbjct:   58 LLRVNVRDENKIPKSRTPDWKYANSLIYKRPFSGEFMKFCLERFEVGIWSSAMEHNVDAALD 119

[Close]

BLAST of FG538273 vs. Lotus protein
Match: LjSGA_117597.1 (+ phase: 0 /pseudo/partial)

HSP 1 Score: 50.0618 bits (118), Expect = 2.307e-7
Identity = 28/68 (41.18%), Postives = 36/68 (52.94%), Query Frame = -3

Query:   17 GFLLHRVHVRDTKGI--------PKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNI 196
            G L  ++ V D  G+        PK   AD       +FKRPF L  +KFC E+FEVGIWSS  + N+
Sbjct:   11 GSLKKKLLVLDINGLLADIVSPRPKDHKADATIAGRAIFKRPFYLEFLKFCFEKFEVGIWSSRTKKNV 78

[Close]

BLAST of FG538273 vs. Soybean peptides
Match: Glyma19g37470.1|PACid:16314093 ()

HSP 1 Score: 84.7297 bits (208), Expect = 1.971e-17
Identity = 51/105 (48.57%), Postives = 58/105 (55.24%), Query Frame = -3

Query:   14 NSEDDYDDEKDTQEPELDXXXXXXXXXXXXXXXXXXXNGFLLHRVHVRDTKGIPKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAME------HNID 310
            +SED+YD E D    +L+                   NG LL RVH      IPKSR AD  YR +L+FKRPF    MKFCLERFEVGIWSSAM+      HNID
Sbjct:    1 DSEDEYDYETDDSGTDLNLHLEKLNLGPRKKLLIMNLNGLLLLRVHRCSKSEIPKSRAADGHYRGYLVFKRPFSQEFMKFCLERFEVGIWSSAMDFRYENRHNID 105

[Close]

BLAST of FG538273 vs. Soybean peptides
Match: Glyma13g06310.1|PACid:16289833 ()

HSP 1 Score: 49.2914 bits (116), Expect = 9.179e-7
Identity = 22/46 (47.83%), Postives = 29/46 (63.04%), Query Frame = -3

Query:   14 PKSRTADYKYRYFLLFKRPFILLGMKFCLERFEVGIWSSAMEHNID 151
            PK   AD       +FKRP  L  ++FC ++FEVGIWSS M+ N+D
Sbjct:   86 PKDHKADATIARRAIFKRPSYLKFLEFCFDKFEVGIWSSRMKKNVD 131

The following BLAST results are available for this feature:

BLAST of FG538273 vs. TrEMBL
BLAST of FG538273 vs. TAIR peptide
BLAST of FG538273 vs. TAIR peptide
BLAST of FG538273 vs. TrEMBL
BLAST of FG538273 vs. Lotus protein
BLAST of FG538273 vs. Soybean peptides

BLAST of FG538273 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 7


Match Name	E-value	Identity	Description
Q58FZ2_ARATH	2.195e-11	57.38	Putative uncharacterized protein OS=Arabidopsis th...	[more]
Q8H1M2_ARATH	7.060e-10	54.10	Putative uncharacterized protein F1O11.17 OS=Arabi...	[more]
Q9SJQ4_ARATH	9.221e-10	57.89	Putative uncharacterized protein At2g36540 OS=Arab...	[more]
D7LJ49_ARALY	1.204e-9	57.89	NLI interacting factor family protein OS=Arabidops...	[more]
A9P944_POPTR	5.594e-7	45.90	Putative uncharacterized protein OS=Populus tricho...	[more]
B9HVI4_POPTR	9.542e-7	51.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PGJ7_POPTR	9.542e-7	51.72	Putative uncharacterized protein OS=Populus tricho...	[more]

BLAST of FG538273 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G36540.1	3.547e-12	57.89	\| Symbols: \| Haloacid dehalogenase-like hydrolase...	[more]

BLAST of FG538273 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G36540.1	3.547e-12	57.89	\| Symbols: \| Haloacid dehalogenase-like hydrolase...	[more]

BLAST of FG538273 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 5


Match Name	E-value	Identity	Description
Q58FZ2_ARATH	2.745e-11	57.38	Putative uncharacterized protein OS=Arabidopsis th...	[more]
Q8H1M2_ARATH	8.829e-10	54.10	Putative uncharacterized protein F1O11.17 OS=Arabi...	[more]
Q9SJQ4_ARATH	1.153e-9	57.89	Putative uncharacterized protein At2g36540 OS=Arab...	[more]
D7LJ49_ARALY	1.506e-9	57.89	NLI interacting factor family protein OS=Arabidops...	[more]
A9P944_POPTR	6.996e-7	45.90	Putative uncharacterized protein OS=Populus tricho...	[more]

BLAST of FG538273 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 2


Match Name	E-value	Identity	Description
chr1.CM0378.310.r2.m	8.453e-18	67.74	- phase: 1 /pseudo/partial	[more]
LjSGA_117597.1	2.307e-7	41.18	+ phase: 0 /pseudo/partial	[more]

BLAST of FG538273 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2


Match Name	E-value	Identity	Description
Glyma19g37470.1\|PACid:16314093	1.971e-17	48.57		[more]
Glyma13g06310.1\|PACid:16289833	9.179e-7	47.83		[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 43..59 score: NAcoord: 66..73 score
None	No IPR available	SEG	seg	seg	coord: 25..44 score
None	No IPR available	SUPERFAMILY	56784	HAD-like	coord: 33..105 score: 3.9
IPR004274	NLI interacting factor	PFAM	PF03031	NIF	coord: 37..105 score: 4.8
IPR004274	NLI interacting factor	PROFILE	PS50969	FCP1	coord: 32..107 score: 11

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 43..59 score: NAcoord: 66..73 score
None	No IPR available	SEG	seg	seg	coord: 25..44 score
None	No IPR available	SUPERFAMILY	56784	HAD-like	coord: 33..105 score: 3.9
IPR004274	NLI interacting factor	PFAM	PF03031	NIF	coord: 37..105 score: 4.8
IPR004274	NLI interacting factor	PROFILE	PS50969	FCP1	coord: 32..107 score: 11

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG538273 ID=FG538273; Name=FG538273; organism=Pisum sativum; type=EST; length=330bp
CATCTCACTGACAATCAATATTATGTTCCATTGCAGAAGACCAGATTCCA
ACTTCAAATCTTTCTAAGCAGAATTTCATGCCTAAAAGAATAAACGGCCT
CTTAAAAAGGAGAAAGTAACGGTATTTGTAATCAGCAGTTCGAGATTTTG
GAATTCCTTTCGTATCACGAACATGAACTCTGTGGAGAAGAAAGCCATTA
AGGTTCATAATAAGCAACTTCTTCTTAGGATTAAGATTCAGCTTCTCAAG
AGAAAGATCCAATTCTGGTTCTTGTGTGTCTTTTTCATCATCATAATCAT
CTTCAGAGTTGCTGCCATAACGGACACTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR004274	FCP1_dom

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