AM161751

EST Overview


Name	AM161751
Unique Name	AM161751
Type	EST
Organism	Pisum sativum (pea)
Sequence length	445

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of AM161751 vs. TrEMBL
Match: C6SY07_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 2.153e-51
Identity = 104/141 (73.76%), Postives = 117/141 (82.98%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMD-ASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF + YHLCQLAHPLLK SGYGSIVF SSIAGLKA+P  SVYA++KGA+NQFTKN+ALEWAKDNIRANAVAPG V T LL SIM  A+E    VE I SQTP+GR+G+  +IS LVAFLCLPAASYITGQ+I  DGGY +
Sbjct:  126 TNFGSSYHLCQLAHPLLKASGYGSIVFISSIAGLKALPYSSVYASSKGAMNQFTKNIALEWAKDNIRANAVAPGTVKTVLLDSIMKAAAEADKAVEYIVSQTPVGRLGDPEDISPLVAFLCLPAASYITGQIITADGGYII 266

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BLAST of AM161751 vs. TrEMBL
Match: A4GXI2_PEA (Oxidoreductase-like protein (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 4.796e-51
Identity = 102/141 (72.34%), Postives = 119/141 (84.40%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIM-DASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNFV+GYHLCQL+HPLLKESGYGSIVF SSIAGLKA+ I S YA +KGA+NQFTKN+ALEWAKDNIRAN VAPGPV T LL + M D +     VE I SQ+P+GRMGE+ +IS LVAFLCLPA+S+ITGQ+IA DGG+T+
Sbjct:   37 TNFVSGYHLCQLSHPLLKESGYGSIVFISSIAGLKALDISSAYAASKGAMNQFTKNVALEWAKDNIRANVVAPGPVKTLLLENAMKDLAGVDKAVENIVSQSPVGRMGESEDISGLVAFLCLPASSHITGQIIAADGGFTM 177

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BLAST of AM161751 vs. TrEMBL
Match: B7FIZ7_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.352e-45
Identity = 91/138 (65.94%), Postives = 111/138 (80.43%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            NF + YHLCQLAHPLLKESGYGSIV  SSI GL+ +P+ S+YA +KGA+NQ TKN+ALE+ KDNIRAN VAPG V T LL SI++  +   V+E   SQTP+ R+ + R+IS+LVAFLCLPAASYITGQ+IA DGG+T
Sbjct:  126 NFESSYHLCQLAHPLLKESGYGSIVSISSILGLRPLPLCSIYAASKGAINQCTKNIALEYGKDNIRANVVAPGAVMTTLLESILEHPDAPKVMEVALSQTPINRVAQPRDISALVAFLCLPAASYITGQIIAADGGFT 263

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BLAST of AM161751 vs. TrEMBL
Match: B7FJU1_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 8.200e-43
Identity = 88/106 (83.02%), Postives = 95/106 (89.62%), Query Frame = 1

Query:  100 LKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            LKAIP+LSVYA TKGAVNQFTKNLALEWAKDNIRANAVAPGPV T LL SI + +EG   V+G+ SQTPMGRMGE +EISSLVAFLCLPAASYITGQVIAIDGG+T
Sbjct:  110 LKAIPVLSVYAATKGAVNQFTKNLALEWAKDNIRANAVAPGPVKTSLLQSITNDNEGDEAVDGVVSQTPMGRMGEPKEISSLVAFLCLPAASYITGQVIAIDGGFT 215

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BLAST of AM161751 vs. TrEMBL
Match: D7THJ2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034114001 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 2.386e-42
Identity = 84/140 (60.00%), Postives = 106/140 (75.71%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF + YHL QL +PLLK SG GSIVF SS+AG+ A+P++S+YA +KGA+NQ T+NLA EWA+DNIR N +AP  +NT L+    D       ++ I S+TP+ RMGE  E+SSLVAFLC PAASYITGQVI +DGGY+V
Sbjct:  120 TNFESAYHLSQLGYPLLKASGRGSIVFISSVAGVMALPVISIYAASKGAMNQVTRNLACEWAEDNIRVNTIAPWVINTSLIHKAKDDPSSEEKIKRIISRTPICRMGEPDEVSSLVAFLCFPAASYITGQVICVDGGYSV 259

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BLAST of AM161751 vs. TrEMBL
Match: D7TLP2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016487001 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.184e-41
Identity = 85/140 (60.71%), Postives = 105/140 (75.00%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  + YHLCQLAHPLLK SG GSIVF SS+AG+ ++   S+YA TK A+NQ TKN A EWAKDNIR+N+VAP  + T L+  ++   +     EGI S+TP+GR GE +E++SLVAFLCLP ASYITGQVI +DGG TV
Sbjct:  125 TNLESAYHLCQLAHPLLKASGAGSIVFVSSVAGVVSLGTGSIYAATKAAINQLTKNFACEWAKDNIRSNSVAPWYIKTSLVEHLLQKKD---FFEGIVSRTPLGRPGEPKEVASLVAFLCLPVASYITGQVICVDGGMTV 261

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BLAST of AM161751 vs. TrEMBL
Match: A9PEY3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_706088 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.184e-41
Identity = 85/140 (60.71%), Postives = 110/140 (78.57%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF + YHLCQLAHPLLK SG G+IVF SS++G+ ++ + S+Y  TKGA+NQ TKNLA EWAKDNIRAN+VAP  + T L+   +   E    ++ + ++TPMGR+GE +E+SSLVAFLC+PAASYITGQVI +DGG+TV
Sbjct:  126 TNFESAYHLCQLAHPLLKNSGAGNIVFMSSVSGVVSVSV-SLYGATKGAINQLTKNLACEWAKDNIRANSVAPWLIRTPLVERDL---ENELFLKAVEARTPMGRLGEPKEVSSLVAFLCMPAASYITGQVICVDGGFTV 261

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BLAST of AM161751 vs. TrEMBL
Match: Q8VXN5_CALSE (Tropinone reductase I OS=Calystegia sepium GN=trI PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.546e-41
Identity = 83/140 (59.29%), Postives = 103/140 (73.57%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF A +HLCQLAHP LK S  G+IVF SS+AG+  +   ++YA +KGA+NQ TKNLA EWAKDNIR N+VAP  + T+L+      SEG   +E +  +TP+ R GE  E+SSLVAFLCLPAASYITGQ I +DGGYT+
Sbjct:  120 TNFEASFHLCQLAHPFLKASSMGTIVFNSSVAGIIPVFKTTLYAASKGAINQVTKNLACEWAKDNIRVNSVAPWVIRTKLVNDFDSESEGSEEIERMIRRTPISRPGEVGEVSSLVAFLCLPAASYITGQTICVDGGYTI 259

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BLAST of AM161751 vs. TrEMBL
Match: B9SDZ5_RICCO (Tropinone reductase, putative OS=Ricinus communis GN=RCOM_1480890 PE=3 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 2.020e-41
Identity = 85/139 (61.15%), Postives = 106/139 (76.26%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            TNF + YH+CQLAHPLLK SG GSIVF SS+AGL+ I   S+Y  TKGA++Q TKNLA EWAKDNIR N+VAP  + T L+  ++   E    VE + ++TP+ R+GE  E+SSLVAFLCLPAASYITGQ+I++DGG T
Sbjct:  125 TNFESAYHMCQLAHPLLKASGVGSIVFISSVAGLQHIGSGSIYGATKGAIHQLTKNLACEWAKDNIRTNSVAPWYIRTSLVERLLKIKE---FVEKVVAKTPLKRIGEPTEVSSLVAFLCLPAASYITGQIISVDGGMT 260

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BLAST of AM161751 vs. TrEMBL
Match: B2BXP6_9ROSI (TRL5 OS=Cleome spinosa GN=TRL5 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.638e-41
Identity = 85/140 (60.71%), Postives = 109/140 (77.86%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF + +HL QLAHPLLK SG GSIVF SSI G+ +IP+ S+Y+ TKGA+NQ  +NLA EWA D+IRANAVAP  V T L+ S+++ +    +VE + S+TP+GR GE +EI++LV FLCLPAASYITGQ I +DGG+TV
Sbjct:  117 TNFESAFHLSQLAHPLLKASGSGSIVFISSITGVVSIPVGSIYSATKGALNQLARNLACEWASDSIRANAVAPNIVLTPLVQSVLNEN----IVEKLESRTPLGRAGEPKEIAALVTFLCLPAASYITGQTICVDGGFTV 252

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BLAST of AM161751 vs. SwissProt
Match: TRNH_DATST (Tropinone reductase homolog OS=Datura stramonium PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 5.759e-42
Identity = 82/140 (58.57%), Postives = 102/140 (72.86%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF A Y+LCQLAHPLLK SG  SIVF SS AG+ A+P+ S+YA +KGA+NQ TK+LA EWAKD+IR NAVAP  +NT ++ +          +E +  + PM R GE  E+SSLV +LCLP ASYITGQ+I +DGGYTV
Sbjct:  125 TNFEASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAKDSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPTASYITGQIICVDGGYTV 264

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BLAST of AM161751 vs. SwissProt
Match: TRN1_DATST (Tropinone reductase 1 OS=Datura stramonium GN=TR1 PE=1 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 2.958e-38
Identity = 77/140 (55.00%), Postives = 103/140 (73.57%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELL-GSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            TNF A YHL Q+A+PLLK S  G+++F SSIAG  A+P +S+Y+ +KGA+NQ TK+LA EWAKDNIR N+VAPG + T L+  +I         ++    +TPMGR G+ +E+S+L+AFLC PAASYITGQ+I  DGG+T
Sbjct:  129 TNFEAAYHLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASKGAINQMTKSLACEWAKDNIRVNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIAFLCFPAASYITGQIIWADGGFT 268

[Close]

BLAST of AM161751 vs. SwissProt
Match: TRNH1_ARATH (Tropinone reductase homolog At1g07440 OS=Arabidopsis thaliana GN=At1g07440 PE=1 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 3.863e-38
Identity = 79/140 (56.43%), Postives = 99/140 (70.71%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  + YHL QLAHPLLK SG G+I+F SSIAG+ +  + S+Y+ TKGA+NQ  +NLA EWA D IRANAVAP  + T L  ++ D        + + S+ P+GR GE  E+SSLVAFLC+PAASYITGQ I +DGG TV
Sbjct:  122 TNLESAYHLSQLAHPLLKASGCGNIIFMSSIAGVVSASVGSIYSATKGALNQLARNLACEWASDGIRANAVAPAVIATPLAEAVYDDE----FKKVVISRKPLGRFGEPEEVSSLVAFLCMPAASYITGQTICVDGGLTV 257

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BLAST of AM161751 vs. SwissProt
Match: TRN2_HYONI (Tropinone reductase 2 OS=Hyoscyamus niger GN=TR2 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 7.538e-34
Identity = 68/137 (49.64%), Postives = 94/137 (68.61%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGY 414
            NF A YHL  LAHP LK S  G++VF SSI+G  A+P  +VY  TKGA++Q T+ LA EWAKDNIR N V PG + T ++   +   E    ++ +  +  + RMGE +E++++VAFLC PAASY+TGQ+I +DGG+
Sbjct:  118 NFEAAYHLSVLAHPFLKASERGNVVFISSISGASALPYEAVYGATKGAMDQLTRCLAFEWAKDNIRVNGVGPGVIATSMVEMTIQDPEQKENLDKLIDRCALRRMGEPKELAAVVAFLCFPAASYVTGQIIYVDGGF 254

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BLAST of AM161751 vs. SwissProt
Match: TRN2_DATST (Tropinone reductase 2 OS=Datura stramonium GN=TR2 PE=1 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 2.193e-33
Identity = 68/136 (50.00%), Postives = 92/136 (67.65%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGG 411
            NF A YHL  LAHP LK S  G++VF SS++G  A+P  +VY  TKGA++Q T+ LA EWAKDNIR N V PG + T L+   +   E    +  +  +  + RMGE +E++++VAFLC PAASY+TGQ+I +DGG
Sbjct:  118 NFEAAYHLSVLAHPFLKASERGNVVFISSVSGALAVPYEAVYGATKGAMDQLTRCLAFEWAKDNIRVNGVGPGVIATSLVEMTIQDPEQKENLNKLIDRCALRRMGEPKELAAMVAFLCFPAASYVTGQIIYVDGG 253

[Close]

BLAST of AM161751 vs. SwissProt
Match: HDHA_ECOLI (7-alpha-hydroxysteroid dehydrogenase OS=Escherichia coli (strain K12) GN=hdhA PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 5.799e-18
Identity = 43/136 (31.62%), Postives = 82/136 (60.29%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGG 411
            N  + +HL QL  P ++++G G I+  +S+A       ++ YA++K A +   +N+A +  + NIR N +APG + T+ L S++       + + +   TP+ R+G+ ++I++   FLC PAAS+++GQ++ + GG
Sbjct:  118 NVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPE----IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG 249

[Close]

BLAST of AM161751 vs. SwissProt
Match: HDHA_ECO57 (7-alpha-hydroxysteroid dehydrogenase OS=Escherichia coli O157:H7 GN=hdhA PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 5.799e-18
Identity = 43/136 (31.62%), Postives = 82/136 (60.29%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGG 411
            N  + +HL QL  P ++++G G I+  +S+A       ++ YA++K A +   +N+A +  + NIR N +APG + T+ L S++       + + +   TP+ R+G+ ++I++   FLC PAAS+++GQ++ + GG
Sbjct:  118 NVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPE----IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG 249

[Close]

BLAST of AM161751 vs. SwissProt
Match: BACC2_BACSU (Bacilysin biosynthesis oxidoreductase BacC OS=Bacillus subtilis GN=bacC PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.204e-17
Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTEL-LGSIMDASEG--GGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            N    + + + A   + +SG G+I+ T S+ G+ A P +  Y  +KG V Q T+++A+++AK NIR N V PG ++T L   S ++ +EG    + +  A   P+ R+G+  EI++++ FL    +SY+TG  I  DGGYT
Sbjct:  111 NLTGMFLMSKHALKYMLKSGKGNIINTCSVGGVVAWPDIPAYNASKGGVLQLTRSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVNPLLRLGKPEEIANVMLFLASDLSSYMTGSAITADGGYT 251

[Close]

BLAST of AM161751 vs. SwissProt
Match: FABG_THEMA (3-oxoacyl-[acyl-carrier-protein] reductase OS=Thermotoga maritima GN=fabG PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.759e-17
Identity = 50/139 (35.97%), Postives = 75/139 (53.96%), Query Frame = 1

Query:    4 NFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            N    +++ Q+  P + +   GSIV  SS+ G+   P  + YA +K  V   TK  A E A  NIR NAVAPG + T +   + + +      E   S+ P+GR G+  E++ ++ FL    +SY+TGQVI IDGG  +
Sbjct:  113 NLKGVFNVTQMVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAVAPGFIETPMTEKLPEKAR-----ETALSRIPLGRFGKPEEVAQVILFLASDESSYVTGQVIGIDGGLVI 246

[Close]

BLAST of AM161751 vs. SwissProt
Match: FABG_CUPLA (3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic OS=Cuphea lanceolata GN=CLKR27 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 4.909e-17
Identity = 51/139 (36.69%), Postives = 78/139 (56.12%), Query Frame = 1

Query:   10 VAGYHLC-QLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCL-PAASYITGQVIAIDGGYTV 420
            + G  LC Q A  ++ +   G I+  +S+ GL      + Y+  K  V  FTK +A E+A  NI  NAVAPG +++++   +     G  + + I    P+GR G+  E++ LV FL + PA+SY+TGQV  IDGG T+
Sbjct:  187 LTGVFLCTQAAAKIMMKKKKGRIINIASVVGLVGNAGQANYSAAKAGVIGFTKTVAREYASRNINVNAVAPGFISSDMTSKL-----GDDINKKILETIPLGRYGQPEEVAGLVEFLAINPASSYVTGQVFTIDGGMTM 320

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT5G06060.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr5:1824066-1825833 REVERSE LENGTH=264)

HSP 1 Score: 169.859 bits (429), Expect = 5.256e-43
Identity = 83/140 (59.29%), Postives = 106/140 (75.71%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  + +HL Q+AHPLLK SG GSIVF SS+AGL  +   S+Y  TKGA+NQ T+NLA EWA DNIR N VAP  + T L+ ++++  E    VE + S+TP+GR+GE  E+SSLVAFLCLPA+SYITGQVI++DGG+TV
Sbjct:  119 TNLESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCVAPWYIKTSLVETLLEKKE---FVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTV 255

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29320.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12592180-12593377 FORWARD LENGTH=269)

HSP 1 Score: 165.622 bits (418), Expect = 9.913e-42
Identity = 87/141 (61.70%), Postives = 101/141 (71.63%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKA-IPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  A YH CQL+HPLLK SGYGSIVF SS+AG+ + I   S+Y  TKGA+NQ  +NLA EWAKD IRANAVAP  V T    S +   E     EG+ S+TP+GR+GE  E+SSLV FLCLPAASYITGQ I +DGG TV
Sbjct:  123 TNLEAAYHFCQLSHPLLKASGYGSIVFLSSVAGVVSLIDCGSIYGLTKGALNQLARNLACEWAKDGIRANAVAPNVVKTAQSQSFL---EDVSKKEGLLSRTPLGRVGEPNEVSSLVVFLCLPAASYITGQTICVDGGLTV 260

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29340.1 (| Symbols: | NAD-dependent epimerase/dehydratase family protein | chr2:12597131-12599009 FORWARD LENGTH=307)

HSP 1 Score: 161.384 bits (407), Expect = 1.869e-40
Identity = 83/140 (59.29%), Postives = 100/140 (71.43%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            +N  A YH  QL+HPLLK SGYGSI+F SSIAG+ +    S+Y  TKGA+ Q  KNLA EWAKD IRANAVAP  +NT L  S +   E     + + S+TP+GR+GE  E++SLVAFLCLPAASYITGQ I +DGG TV
Sbjct:  117 SNVEAAYHFSQLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGIRANAVAPNVINTPLSQSYL---EDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITGQTICVDGGLTV 253

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29340.2 (| Symbols: | NAD-dependent epimerase/dehydratase family protein | chr2:12597131-12598306 FORWARD LENGTH=262)

HSP 1 Score: 161.384 bits (407), Expect = 1.869e-40
Identity = 83/140 (59.29%), Postives = 100/140 (71.43%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            +N  A YH  QL+HPLLK SGYGSI+F SSIAG+ +    S+Y  TKGA+ Q  KNLA EWAKD IRANAVAP  +NT L  S +   E     + + S+TP+GR+GE  E++SLVAFLCLPAASYITGQ I +DGG TV
Sbjct:  117 SNVEAAYHFSQLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGIRANAVAPNVINTPLSQSYL---EDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITGQTICVDGGLTV 253

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29260.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12582523-12583954 FORWARD LENGTH=322)

HSP 1 Score: 160.614 bits (405), Expect = 3.189e-40
Identity = 76/140 (54.29%), Postives = 107/140 (76.43%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TNF + +HLCQLA+PLL+ES  GS+VF SS++G  ++  +SV ++TKGA+NQ T++LA EWAKDNIR NAVAP  + T ++  ++   E    +E + S TP+GR+GE RE+SS VAFLCLPA+SYITGQ++ +DGG ++
Sbjct:  178 TNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKE---YLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSI 314

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29300.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12588214-12589372 FORWARD LENGTH=263)

HSP 1 Score: 160.229 bits (404), Expect = 4.165e-40
Identity = 86/142 (60.56%), Postives = 102/142 (71.83%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPIL-SVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNT-ELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  A YH CQL+HPLLK SGYGSIVF SS++G+ +I    S+Y  TKGA+NQ  +NLA EWAKD IRANAVAP  V T +    + D S+     EG+ S+TP+GR GE  E++SLV FLCLPAASYITGQ I IDGG TV
Sbjct:  117 TNLEAAYHFCQLSHPLLKTSGYGSIVFLSSVSGVVSITDCGSLYGLTKGALNQLARNLACEWAKDGIRANAVAPNVVKTAQSQFFLQDVSKK----EGLFSRTPLGRSGEPNEVASLVVFLCLPAASYITGQTICIDGGLTV 254

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29330.1 (| Symbols: TRI | tropinone reductase | chr2:12594604-12596196 FORWARD LENGTH=260)

HSP 1 Score: 159.458 bits (402), Expect = 7.104e-40
Identity = 82/140 (58.57%), Postives = 98/140 (70.00%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN  + YHL QL+HPLLK SG G I F SS AG+ +    S+Y  TKGA+NQ  +NLA EWAKD IRANAVAP  + T L    +   E  G  E ++S+TP+GR GE RE++SLVAFLCLPAASYITGQ I +DGG TV
Sbjct:  117 TNLESAYHLSQLSHPLLKASGNGIITFISSAAGIVSFDAASIYGLTKGALNQLARNLACEWAKDGIRANAVAPNFITTALAKPFL---EDAGFNEILSSRTPLGRAGEPREVASLVAFLCLPAASYITGQTICVDGGLTV 253

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29300.2 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12588214-12589643 FORWARD LENGTH=286)

HSP 1 Score: 158.688 bits (400), Expect = 1.212e-39
Identity = 85/141 (60.28%), Postives = 101/141 (71.63%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPIL-SVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNT-ELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYT 417
            TN  A YH CQL+HPLLK SGYGSIVF SS++G+ +I    S+Y  TKGA+NQ  +NLA EWAKD IRANAVAP  V T +    + D S+     EG+ S+TP+GR GE  E++SLV FLCLPAASYITGQ I IDGG T
Sbjct:  117 TNLEAAYHFCQLSHPLLKTSGYGSIVFLSSVSGVVSITDCGSLYGLTKGALNQLARNLACEWAKDGIRANAVAPNVVKTAQSQFFLQDVSKK----EGLFSRTPLGRSGEPNEVASLVVFLCLPAASYITGQTICIDGGLT 253

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29310.2 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12590079-12591260 FORWARD LENGTH=260)

HSP 1 Score: 158.303 bits (399), Expect = 1.583e-39
Identity = 82/140 (58.57%), Postives = 99/140 (70.71%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN    +H  QL+H LLK SG+GSIVF SS  G+ ++   S+Y+ TKGA+NQ T+NLA EWAKD IRANAVAP  V T L  S +   E  G  E + S+TP+GR GE  E++SLV FLCLPAASYITGQ I IDGG+TV
Sbjct:  115 TNLEPAFHFSQLSHLLLKASGFGSIVFMSSATGVVSVQCGSIYSLTKGALNQLTRNLACEWAKDGIRANAVAPNVVKTPLSQSYL---EDVGFKEALFSRTPLGRAGEPNEVASLVVFLCLPAASYITGQTICIDGGFTV 251

[Close]

BLAST of AM161751 vs. TAIR peptide
Match: AT2G29310.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:12590068-12591260 FORWARD LENGTH=262)

HSP 1 Score: 158.303 bits (399), Expect = 1.583e-39
Identity = 82/140 (58.57%), Postives = 99/140 (70.71%), Query Frame = 1

Query:    1 TNFVAGYHLCQLAHPLLKESGYGSIVFTSSIAGLKAIPILSVYATTKGAVNQFTKNLALEWAKDNIRANAVAPGPVNTELLGSIMDASEGGGVVEGIASQTPMGRMGETREISSLVAFLCLPAASYITGQVIAIDGGYTV 420
            TN    +H  QL+H LLK SG+GSIVF SS  G+ ++   S+Y+ TKGA+NQ T+NLA EWAKD IRANAVAP  V T L  S +   E  G  E + S+TP+GR GE  E++SLV FLCLPAASYITGQ I IDGG+TV
Sbjct:  117 TNLEPAFHFSQLSHLLLKASGFGSIVFMSSATGVVSVQCGSIYSLTKGALNQLTRNLACEWAKDGIRANAVAPNVVKTPLSQSYL---EDVGFKEALFSRTPLGRAGEPNEVASLVVFLCLPAASYITGQTICIDGGFTV 253

The following BLAST results are available for this feature:

BLAST of AM161751 vs. TrEMBL
BLAST of AM161751 vs. SwissProt
BLAST of AM161751 vs. TAIR peptide

BLAST of AM161751 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SY07_SOYBN	2.153e-51	73.76	Putative uncharacterized protein OS=Glycine max PE...	[more]
A4GXI2_PEA	4.796e-51	72.34	Oxidoreductase-like protein (Fragment) OS=Pisum sa...	[more]
B7FIZ7_MEDTR	1.352e-45	65.94	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FJU1_MEDTR	8.200e-43	83.02	Putative uncharacterized protein OS=Medicago trunc...	[more]
D7THJ2_VITVI	2.386e-42	60.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TLP2_VITVI	1.184e-41	60.71	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9PEY3_POPTR	1.184e-41	60.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8VXN5_CALSE	1.546e-41	59.29	Tropinone reductase I OS=Calystegia sepium GN=trI ...	[more]
B9SDZ5_RICCO	2.020e-41	61.15	Tropinone reductase, putative OS=Ricinus communis ...	[more]
B2BXP6_9ROSI	2.638e-41	60.71	TRL5 OS=Cleome spinosa GN=TRL5 PE=3 SV=1	[more]

BLAST of AM161751 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TRNH_DATST	5.759e-42	58.57	Tropinone reductase homolog OS=Datura stramonium P...	[more]
TRN1_DATST	2.958e-38	55.00	Tropinone reductase 1 OS=Datura stramonium GN=TR1 ...	[more]
TRNH1_ARATH	3.863e-38	56.43	Tropinone reductase homolog At1g07440 OS=Arabidops...	[more]
TRN2_HYONI	7.538e-34	49.64	Tropinone reductase 2 OS=Hyoscyamus niger GN=TR2 P...	[more]
TRN2_DATST	2.193e-33	50.00	Tropinone reductase 2 OS=Datura stramonium GN=TR2 ...	[more]
HDHA_ECOLI	5.799e-18	31.62	7-alpha-hydroxysteroid dehydrogenase OS=Escherichi...	[more]
HDHA_ECO57	5.799e-18	31.62	7-alpha-hydroxysteroid dehydrogenase OS=Escherichi...	[more]
BACC2_BACSU	2.204e-17	35.46	Bacilysin biosynthesis oxidoreductase BacC OS=Baci...	[more]
FABG_THEMA	3.759e-17	35.97	3-oxoacyl-[acyl-carrier-protein] reductase OS=Ther...	[more]
FABG_CUPLA	4.909e-17	36.69	3-oxoacyl-[acyl-carrier-protein] reductase, chloro...	[more]

BLAST of AM161751 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G06060.1	5.256e-43	59.29	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29320.1	9.913e-42	61.70	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29340.1	1.869e-40	59.29	\| Symbols: \| NAD-dependent epimerase/dehydratase ...	[more]
AT2G29340.2	1.869e-40	59.29	\| Symbols: \| NAD-dependent epimerase/dehydratase ...	[more]
AT2G29260.1	3.189e-40	54.29	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29300.1	4.165e-40	60.56	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29330.1	7.104e-40	58.57	\| Symbols: TRI \| tropinone reductase \| chr2:125946...	[more]
AT2G29300.2	1.212e-39	60.28	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29310.2	1.583e-39	58.57	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT2G29310.1	1.583e-39	58.57	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002198	Short-chain dehydrogenase/reductase SDR	PANTHER	PTHR19410	SHORT-CHAIN DEHYDROGENASES/REDUCTASE FAMILY MEMBER	coord: 1..136 score: 1.4
IPR002198	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 2..61 score: 7.8
IPR002347	Glucose/ribitol dehydrogenase	PRINTS	PR00081	GDHRDH	coord: 17..33 score: 2.9e-25coord: 43..62 score: 2.9e-25coord: 64..81 score: 2.9e-25coord: 102..122 score: 2.9
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 1..140 score: 8.5
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 30..58 score
None	No IPR available	PANTHER	PTHR19410:SF66	TROPINONE REDUCTASE	coord: 1..136 score: 1.4
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 1..140 score: 3.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161751 ID=AM161751; Name=AM161751; organism=Pisum sativum; type=EST; length=445bp
ACCAATTTTGTGGCCGGTTACCATTTATGTCAACTTGCACACCCACTTCT
AAAAGAGTCTGGATATGGAAGCATAGTGTTTACTTCCTCCATTGCTGGTT
TAAAAGCTATTCCTATTTTATCTGTTTATGCAACAACTAAAGGTGCGGTG
AATCAATTCACCAAAAACTTGGCATTAGAATGGGCAAAGGATAATATTCG
TGCAAATGCTGTTGCACCTGGACCTGTCAATACTGAACTTTTGGGATCAA
TAATGGATGCTTCTGAAGGGGGTGGAGTTGTAGAGGGTATCGCGTCTCAA
ACTCCTATGGGTCGTATGGGAGAGACTAGAGAAATATCATCATTAGTTGC
TTTTCTTTGTCTTCCAGCTGCATCATACATTACTGGACAAGTTATAGCTA
TTGATGGGGGTTACACTGTTTAGATCAACGCTATTGATATATTTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002198	DH_sc/Rdtase_SDR
IPR002347	SDR_fam
IPR016040	NAD(P)-bd_dom
IPR020904	Sc_DH/Rdtase_CS

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