AM162130

EST Overview


Name	AM162130
Unique Name	AM162130
Type	EST
Organism	Pisum sativum (pea)
Sequence length	480

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162130 vs. TrEMBL
Match: B9HCJ7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_560878 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 9.135e-38
Identity = 77/105 (73.33%), Postives = 89/105 (84.76%), Query Frame = -1

Query:  100 NKRSSALCLCKFNESDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEINST 411
            N ++S L LCK N+SDS+AP P GDTKKQELLA+ AMLQ +K+RLTDYLDERS YLTQF E+ NAEF+KIGEDALKGLDEAGARI  N+ESQ+  FEESAE+N T
Sbjct:   26 NTKNSILFLCKSNDSDSEAPPPEGDTKKQELLAKIAMLQAQKVRLTDYLDERSDYLTQFAEEANAEFDKIGEDALKGLDEAGARIMENIESQMQAFEESAELNRT 130

HSP 2 Score: 30.0314 bits (66), Expect = 9.135e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = -2

Query:    3 MENSRNEGLFFKNLRKK 53
            MEN RNEG+FFK+L +K
Sbjct:  146 MENDRNEGMFFKSLGQK 162

[Close]

BLAST of AM162130 vs. TrEMBL
Match: A9PJB2_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 9.135e-38
Identity = 77/105 (73.33%), Postives = 89/105 (84.76%), Query Frame = -1

Query:  100 NKRSSALCLCKFNESDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEINST 411
            N ++S L LCK N+SDS+AP P GDTKKQELLA+ AMLQ +K+RLTDYLDERS YLTQF E+ NAEF+KIGEDALKGLDEAGARI  N+ESQ+  FEESAE+N T
Sbjct:   26 NTKNSILFLCKSNDSDSEAPPPEGDTKKQELLAKIAMLQAQKVRLTDYLDERSDYLTQFAEEANAEFDKIGEDALKGLDEAGARILENIESQMQAFEESAELNRT 130

HSP 2 Score: 30.0314 bits (66), Expect = 9.135e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = -2

Query:    3 MENSRNEGLFFKNLRKK 53
            MEN RNEG+FFK+L +K
Sbjct:  146 MENDRNEGMFFKSLGQK 162

[Close]

BLAST of AM162130 vs. TrEMBL
Match: B9T6M3_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0098840 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 5.026e-32
Identity = 71/111 (63.96%), Postives = 88/111 (79.28%), Query Frame = -1

Query:  106 NKRSSALCLCKFNE--------SDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEIN 411
            N++++  CLCK N         SDS+A  P GDT+KQELLA+ AMLQ  K+RLTDY+DERS+YL+QF E+ +AEF+KIGEDALKGLDEAGARI  N+ESQ+L FEESAE+N
Sbjct:   26 NQKNTIWCLCKPNNNNSDSNSNSDSEASPPEGDTRKQELLARIAMLQTEKVRLTDYIDERSSYLSQFTEEASAEFDKIGEDALKGLDEAGARIMENIESQMLAFEESAELN 136

HSP 2 Score: 25.7942 bits (55), Expect = 5.026e-32
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = -2

Query:    3 RNEGLFFKNLRKK 41
            RNEG+FFKNL +K
Sbjct:  158 RNEGMFFKNLGQK 170

[Close]

BLAST of AM162130 vs. TrEMBL
Match: D7THA8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034004001 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.576e-30
Identity = 64/105 (60.95%), Postives = 81/105 (77.14%), Query Frame = -1

Query:  106 KRSSALCLCKFNESDSKAPQ----PGDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEIN 408
            K+ S L LCK N+ DS         GD++KQELLA+ AMLQ +K+RLTDYLDERSAYLTQF E+ NAEF++IGE+ALK L+EAG RI  NMESQ+  F+ES+E++
Sbjct: 1222 KKRSTLSLCKSNDDDSSPSDGSLPEGDSRKQELLARIAMLQAQKVRLTDYLDERSAYLTQFAEEANAEFDQIGENALKELEEAGTRIMENMESQMQAFQESSEMS 1326

HSP 2 Score: 30.4166 bits (67), Expect = 2.576e-30
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = -2

Query:    3 MENSRNEGLFFKNLRKK 53
            +E  RNEGLFFKNLR++
Sbjct: 1344 IEKERNEGLFFKNLRQR 1360

[Close]

BLAST of AM162130 vs. TrEMBL
Match: Q9SS82_ARATH (At3g09050 OS=Arabidopsis thaliana GN=MZB10.8 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.531e-26
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = -1

Query:  106 RSSALCLCKF------NESDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEIN 405
            R   LCL K       ++SD   P+P GDT++QELLA+ AM+Q  K+RLTD+LDERS YLT+F E+ NAEF+K+GEDA+K LDEA  RI  N+ES++  FEESA +N
Sbjct:   29 RPQFLCLSKSGDGTSDSDSDPDPPKPEGDTRRQELLARIAMIQTSKVRLTDFLDERSEYLTKFAEEANAEFDKVGEDAMKDLDEASTRILENIESKMQAFEESAGLN 135

HSP 2 Score: 26.5646 bits (57), Expect = 3.531e-26
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:    3 RNEGLFFKNLRKK 41
            RNEGLFFK+LR K
Sbjct:  157 RNEGLFFKSLRDK 169

[Close]

BLAST of AM162130 vs. TrEMBL
Match: D7L7R0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478215 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 5.986e-26
Identity = 59/107 (55.14%), Postives = 78/107 (72.90%), Query Frame = -1

Query:  106 RSSALCLCKF------NESDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEIN 405
            R+  LCL K       ++SD   P+P GDT++QELLA+ AM+Q  K+RLTD+LDERS YLT+F E+ N EF+K+GEDA+K LDEA  RI  N+ES++  FEESA +N
Sbjct:   29 RTQFLCLSKSGDGTSDSDSDPDPPKPEGDTRRQELLARIAMIQTSKVRLTDFLDERSEYLTKFAEEANVEFDKVGEDAMKDLDEASTRILENIESKMQAFEESAGLN 135

HSP 2 Score: 26.5646 bits (57), Expect = 5.986e-26
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:    3 RNEGLFFKNLRKK 41
            RNEGLFFK+LR K
Sbjct:  157 RNEGLFFKSLRDK 169

[Close]

BLAST of AM162130 vs. TrEMBL
Match: Q5N9C0_ORYSJ (Os01g0853000 protein OS=Oryza sativa subsp. japonica GN=P0529E05.17 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.519e-19
Identity = 47/108 (43.52%), Postives = 75/108 (69.44%), Query Frame = -1

Query:  109 KCNSIYNKRSSA-LCLCKFNESDSKAPQPGDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEI 429
            +C  +  KRS    C  + + SD+ +   GD ++QE+LA+ AMLQ +K+R+T +LDERSAYLT+F +  + EF+ IG++A+K LDE G +I   ++S++  FEE+AE+
Sbjct:   21 RCALLKRKRSCIRACSSEDDGSDASSSLGGDKRQQEVLAKIAMLQAQKVRITSFLDERSAYLTKFAKDADTEFDLIGQNAMKELDEVGDQIMERLDSKMQAFEETAEV 128

HSP 2 Score: 24.6386 bits (52), Expect = 1.519e-19
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = -2

Query:    6 MENSRNEGLFFKNLRK 53
            +E  +NEG+FFK+L K
Sbjct:  147 IEKEKNEGMFFKSLGK 162

[Close]

BLAST of AM162130 vs. TrEMBL
Match: C5XPW0_SORBI (Putative uncharacterized protein Sb03g040140 OS=Sorghum bicolor GN=Sb03g040140 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.571e-19
Identity = 45/106 (42.45%), Postives = 76/106 (71.70%), Query Frame = -1

Query:  109 SIYNKRSSALCLCKFNESDSKAPQP--GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEI 420
            ++  ++ S +C     ++++ +P P  GD  +QE+LAQ AMLQ +K+R+TD+LDERSAYLT+F +  ++EF+ IG++A+K LD  G +I   ++S++  FEE+AE+
Sbjct:   22 ALLGRKRSFICASSSEDAEAGSPPPLGGDKHQQEVLAQIAMLQAQKVRITDFLDERSAYLTKFAKDADSEFDLIGQNAMKELDAVGDQILERLDSKMQAFEETAEV 127

HSP 2 Score: 21.9422 bits (45), Expect = 2.571e-19
Identity = 7/16 (43.75%), Postives = 13/16 (81.25%), Query Frame = -2

Query:    6 MENSRNEGLFFKNLRK 53
            +E  +N+G+FF++L K
Sbjct:  146 IEKEKNQGMFFQSLGK 161

[Close]

BLAST of AM162130 vs. TrEMBL
Match: C4J0W2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.299e-13
Identity = 33/66 (50.00%), Postives = 52/66 (78.79%), Query Frame = -1

Query:  109 MLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEI 306
            MLQ +K+R+TD+LDERSAYLT+F +  ++EF+ IG++A+K LD  G +I   ++S++  FEE+AE+
Sbjct:    1 MLQTQKVRITDFLDERSAYLTKFAKDADSEFDLIGQNAMKELDAVGDQILERLDSKMQAFEETAEV 66

HSP 2 Score: 24.6386 bits (52), Expect = 1.299e-13
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = -2

Query:    6 MENSRNEGLFFKNLRK 53
            +E  +NEG+FFK+L K
Sbjct:   85 IEKEKNEGMFFKSLGK 100

[Close]

BLAST of AM162130 vs. TrEMBL
Match: B8ACF8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04466 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.687e-13
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = -1

Query:  109 MLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEI 306
            MLQ +K+R+T +LDERSAYLT+F +  + EF+ IG++A+K LDE G +I   ++S++  FEE+AE+
Sbjct:    1 MLQAQKVRITSFLDERSAYLTKFAKDADTEFDLIGQNAMKELDEVGDQIMERLDSKMQAFEETAEV 66

HSP 2 Score: 24.6386 bits (52), Expect = 1.687e-13
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = -2

Query:    6 MENSRNEGLFFKNLRK 53
            +E  +NEG+FFK+L K
Sbjct:   85 IEKEKNEGMFFKSLGK 100

[Close]

BLAST of AM162130 vs. TAIR peptide
Match: AT3G09050.1 (| Symbols: | unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; Has 120 Blast hits to 120 proteins in 47 species: Archae - 4; Bacteria - 10; Metazoa - 33; Fungi - 2; Plants - 44; Viruses - 0; Other Eukaryotes - 27 (source: NCBI BLink). | chr3:2764860-2765907 FORWARD LENGTH=258)

HSP 1 Score: 115.546 bits (288), Expect = 2.842e-28
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = -1

Query:  106 RSSALCLCKF------NESDSKAPQP-GDTKKQELLAQTAMLQVRKIRLTDYLDERSAYLTQFGEKVNAEFEKIGEDALKGLDEAGARITANMESQLLEFEESAEIN 405
            R   LCL K       ++SD   P+P GDT++QELLA+ AM+Q  K+RLTD+LDERS YLT+F E+ NAEF+K+GEDA+K LDEA  RI  N+ES++  FEESA +N
Sbjct:   29 RPQFLCLSKSGDGTSDSDSDPDPPKPEGDTRRQELLARIAMIQTSKVRLTDFLDERSEYLTKFAEEANAEFDKVGEDAMKDLDEASTRILENIESKMQAFEESAGLN 135

HSP 2 Score: 26.5646 bits (57), Expect = 2.842e-28
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = -2

Query:    3 RNEGLFFKNLRKK 41
            RNEGLFFK+LR K
Sbjct:  157 RNEGLFFKSLRDK 169

The following BLAST results are available for this feature:

BLAST of AM162130 vs. TrEMBL
BLAST of AM162130 vs. TAIR peptide

BLAST of AM162130 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HCJ7_POPTR	9.135e-38	73.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PJB2_9ROSI	9.135e-38	73.33	Putative uncharacterized protein OS=Populus tricho...	[more]
B9T6M3_RICCO	5.026e-32	63.96	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7THA8_VITVI	2.576e-30	60.95	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9SS82_ARATH	3.531e-26	56.07	At3g09050 OS=Arabidopsis thaliana GN=MZB10.8 PE=2 ...	[more]
D7L7R0_ARALY	5.986e-26	55.14	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q5N9C0_ORYSJ	1.519e-19	43.52	Os01g0853000 protein OS=Oryza sativa subsp. japoni...	[more]
C5XPW0_SORBI	2.571e-19	42.45	Putative uncharacterized protein Sb03g040140 OS=So...	[more]
C4J0W2_MAIZE	1.299e-13	50.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B8ACF8_ORYSI	1.687e-13	50.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of AM162130 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G09050.1	2.842e-28	56.07	\| Symbols: \| unknown protein; FUNCTIONS IN: molec...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 28..42 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162130 ID=AM162130; Name=AM162130; organism=Pisum sativum; type=EST; length=480bp
ACCTTCTTTCTGAGGTTCTTAAAGAATAGCCCTTCATTTCTGCTGTTTTC
CATTTGAACTTCAAACTCCTCCAACTCGTTTTCACGCTCTTGAATCTCTT
GTACTGTTAATCTCTGCAGATTCCTCAAATTCTAGCAGCTGGCTCTCCAT
GTTTGCTGTTATTCTGGCACCAGCTTCATCTAATCCTTTGAGGGCATCTT
CTCCGATCTTTTCAAACTCGGCGTTAACCTTTTCACCAAATTGGGTTAAG
TATGCTGATCTCTCATCTAGATAATCTGTGAGACGGATTTTTCGAACTTG
AAGCATGGCCGTTTGGGCTAGTAGTTCTTGTTTTTTTGTATCTCCTGGTT
GAGGAGCTTTAGAATCAGACTCATTGAACTTGCAGAGGCACAAAGCGGAG
CTTCTTTTGTTGTAGATACTATTACACTTGATGTTCAGTAAGTCTGAAGG
AAGGATTTATGGATTTGAGAAGCAATCATC

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