CD859284

EST Overview


Name	CD859284
Unique Name	CD859284
Type	EST
Organism	Pisum sativum (pea)
Sequence length	656

Libraries


Library Name	Type
LIBEST_013999 CNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of CD859284 vs. TrEMBL
Match: D7SMI3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_83.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036477001 PE=4 SV=1)

HSP 1 Score: 354.755 bits (909), Expect = 3.412e-96
Identity = 166/185 (89.73%), Postives = 178/185 (96.22%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNF GL+FPFIREKL  +INP+IRAVEPTACPSLTKG+YAYD+GDTAG+TPLMKMHTLGHDFVPDPIHAGGLRYHGMAPL+SHVYELG MEAISIPQ ECFQGAIQFAR+EGLIPAPEPTHAIAATIREALHCRETGE+KVILTAMCGHGHFDLPAYEKYLQGNMVDLSF E+++KAS ANIP
Sbjct:  307 GGSNFGGLTFPFIREKLKGRINPLIRAVEPTACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLLSHVYELGFMEAISIPQIECFQGAIQFARTEGLIPAPEPTHAIAATIREALHCRETGESKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFEEEKIKASLANIP 491

[Close]

BLAST of CD859284 vs. TrEMBL
Match: B9N1Y3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_266088 PE=4 SV=1)

HSP 1 Score: 341.658 bits (875), Expect = 2.989e-92
Identity = 159/185 (85.95%), Postives = 174/185 (94.05%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNFAGLSFPFIREKLN KINPVI+AVEP ACPSLTKG+YAYD+GDTAG+TPLMKMHTLGHDF+PDPIHAGGLRYHGMAPLISHVYELG MEA++IPQ ECF+GAIQFARSEGLIPAPEPTHAIAATIREALHC+ETGEAKVIL AMCGHGHFDL +YEKYLQG MVDLSF E++++AS   +P
Sbjct:  275 GGSNFAGLSFPFIREKLNGKINPVIKAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYELGFMEAMAIPQIECFRGAIQFARSEGLIPAPEPTHAIAATIREALHCKETGEAKVILMAMCGHGHFDLKSYEKYLQGKMVDLSFDEEKIRASLDKVP 459

[Close]

BLAST of CD859284 vs. TrEMBL
Match: Q9FFW8_ARATH (Tryptophan synthase beta chain OS=Arabidopsis thaliana GN=At5g38530 PE=1 SV=1)

HSP 1 Score: 336.265 bits (861), Expect = 1.256e-90
Identity = 157/185 (84.86%), Postives = 173/185 (93.51%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNFAGLSFPFIREKL  KINPVIRAVEP+ACPSLTKG+YAYDFGDTAGLTPLMKMHTLGHDF+PDPIHAGGLRYHGMAPLISHVYE G MEAISIPQ ECFQGAIQFAR+EG+IPAPEPTHAIAATIREAL C+ETGEAKVIL AMCGHGHFDL +Y+KYL+G +VDLSFSE++++ S + +P
Sbjct:  319 GGSNFAGLSFPFIREKLKGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKETGEAKVILMAMCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESLSKVP 503

[Close]

BLAST of CD859284 vs. TrEMBL
Match: D7MJX6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_494122 PE=4 SV=1)

HSP 1 Score: 335.109 bits (858), Expect = 2.798e-90
Identity = 156/185 (84.32%), Postives = 172/185 (92.97%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNFAGLSFPFIREKL   INPVIRAVEP+ACPSLTKG+YAYDFGDTAGLTPLMKMHTLGHDF+PDPIHAGGLRYHGMAPLISHVYE G MEAISIPQ ECFQGAIQFAR+EG+IPAPEPTHAIAATIREAL CRETGEAKVIL AMCGHGHFDL +Y+KYL+G ++DLSFSE++++ S + +P
Sbjct:  320 GGSNFAGLSFPFIREKLKGNINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCRETGEAKVILMAMCGHGHFDLASYDKYLKGELIDLSFSEEKIRESLSKVP 504

[Close]

BLAST of CD859284 vs. TrEMBL
Match: A9NUV8_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 1.030e-84
Identity = 145/187 (77.54%), Postives = 167/187 (89.30%), Query Frame = -3

Query:   94 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIPSF 654
            GGSNFAGL+FPFIREKL   +NP IRAVEP ACPSLTKG+Y YD+ DTAG+TPL+KMH+LGHDF+PDPIHAGGLRYHGMAPLISHVY LGLMEAI+IPQ ECFQGA++FAR+EGLIPAPEPTHAIAA IREAL C+ETGEAKVIL A+CGHGHFDL +YEKYL+G MVDL + ++++  + A IPSF
Sbjct:   94 GGSNFAGLAFPFIREKLAGNMNPTIRAVEPAACPSLTKGVYVYDYADTAGMTPLLKMHSLGHDFIPDPIHAGGLRYHGMAPLISHVYNLGLMEAIAIPQIECFQGAVKFARTEGLIPAPEPTHAIAAAIREALQCKETGEAKVILIALCGHGHFDLFSYEKYLKGQMVDLEYPDEKINEAMAKIPSF 280

[Close]

BLAST of CD859284 vs. TrEMBL
Match: A9S4R5_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_124222 PE=4 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.756e-84
Identity = 143/185 (77.30%), Postives = 168/185 (90.81%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNFAGLSFPFIREKL  K+NP+IRAVEP ACPSLT+G+YAYDFGDT+GLTPLMKMHTLGHDF+PDPIHAGGLRYHGMAPL+SHVYELGLMEAI+IPQ ECF+ A++FAR+EG+IPAPEPTHAIAATIREAL C+E+GEAKVI+TA+CGHGHFDL +Y+ +L G MVDL +  D+++ S   +P
Sbjct:  253 GGSNFAGLSFPFIREKLAGKMNPIIRAVEPAACPSLTRGVYAYDFGDTSGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLVSHVYELGLMEAIAIPQVECFEAALKFARTEGIIPAPEPTHAIAATIREALACKESGEAKVIVTALCGHGHFDLASYDTFLAGGMVDLQYPTDKIRESIPKLP 437

[Close]

BLAST of CD859284 vs. TrEMBL
Match: C5Z690_SORBI (Putative uncharacterized protein Sb10g024680 OS=Sorghum bicolor GN=Sb10g024680 PE=4 SV=1)

HSP 1 Score: 310.842 bits (795), Expect = 5.650e-83
Identity = 145/185 (78.38%), Postives = 163/185 (88.11%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNF GL+FPF+REKL   ++P  RAVEP ACP+LTKG+YAYDFGDTAGLTPLMKMHTLGH FVPDPIHAGGLRYHGMAPLISHVYELG M+AI+I QTECFQ A+QFAR+EG+IPAPEPTHAIAA IREAL C+ TGE KVIL AMCGHGHFDL AYEKYL+G+MVDLS   ++L+AS A +P
Sbjct:  306 GGSNFGGLAFPFLREKLRGNMSPAFRAVEPAACPTLTKGVYAYDFGDTAGLTPLMKMHTLGHGFVPDPIHAGGLRYHGMAPLISHVYELGFMDAIAIQQTECFQAALQFARTEGIIPAPEPTHAIAAAIREALECKRTGEEKVILMAMCGHGHFDLAAYEKYLRGDMVDLSHPAEKLEASLAAVP 490

[Close]

BLAST of CD859284 vs. TrEMBL
Match: Q67VM2_ORYSJ (Putative Tryptophan synthase beta chain OS=Oryza sativa subsp. japonica GN=P0505A04.10-1 PE=4 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 6.907e-81
Identity = 140/185 (75.68%), Postives = 163/185 (88.11%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNF GL FPF+REKL  +++P  +AVEP ACP+LTKG+YAYDFGDTAGLTPLMKMHTLGH FVPDPIHAGGLRYHGMAPLISHVYELG MEAI+I QTECF  A++FAR+EG+IPAPEPTHAIAA IREA+ C+ TGE KVIL AMCGHGHFDL +YEKYL+G+MVDLS S+++L+ + A +P
Sbjct:  299 GGSNFGGLVFPFMREKLAGRMSPAFKAVEPAACPTLTKGVYAYDFGDTAGLTPLMKMHTLGHGFVPDPIHAGGLRYHGMAPLISHVYELGFMEAIAIQQTECFDAALKFARTEGIIPAPEPTHAIAAAIREAMECKRTGEKKVILMAMCGHGHFDLASYEKYLRGDMVDLSHSDEKLQEALAAVP 483

[Close]

BLAST of CD859284 vs. TrEMBL
Match: Q67VM1_ORYSJ (Putative Tryptophan synthase beta chain OS=Oryza sativa subsp. japonica GN=P0505A04.10-2 PE=2 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 6.907e-81
Identity = 140/185 (75.68%), Postives = 163/185 (88.11%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNF GL FPF+REKL  +++P  +AVEP ACP+LTKG+YAYDFGDTAGLTPLMKMHTLGH FVPDPIHAGGLRYHGMAPLISHVYELG MEAI+I QTECF  A++FAR+EG+IPAPEPTHAIAA IREA+ C+ TGE KVIL AMCGHGHFDL +YEKYL+G+MVDLS S+++L+ + A +P
Sbjct:  301 GGSNFGGLVFPFMREKLAGRMSPAFKAVEPAACPTLTKGVYAYDFGDTAGLTPLMKMHTLGHGFVPDPIHAGGLRYHGMAPLISHVYELGFMEAIAIQQTECFDAALKFARTEGIIPAPEPTHAIAAAIREAMECKRTGEKKVILMAMCGHGHFDLASYEKYLRGDMVDLSHSDEKLQEALAAVP 485

[Close]

BLAST of CD859284 vs. TrEMBL
Match: A3BDS5_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_22056 PE=4 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 6.907e-81
Identity = 140/185 (75.68%), Postives = 163/185 (88.11%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNF GL FPF+REKL  +++P  +AVEP ACP+LTKG+YAYDFGDTAGLTPLMKMHTLGH FVPDPIHAGGLRYHGMAPLISHVYELG MEAI+I QTECF  A++FAR+EG+IPAPEPTHAIAA IREA+ C+ TGE KVIL AMCGHGHFDL +YEKYL+G+MVDLS S+++L+ + A +P
Sbjct:  301 GGSNFGGLVFPFMREKLAGRMSPAFKAVEPAACPTLTKGVYAYDFGDTAGLTPLMKMHTLGHGFVPDPIHAGGLRYHGMAPLISHVYELGFMEAIAIQQTECFDAALKFARTEGIIPAPEPTHAIAAAIREAMECKRTGEKKVILMAMCGHGHFDLASYEKYLRGDMVDLSHSDEKLQEALAAVP 485

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_AQUAE (Tryptophan synthase beta chain 2 OS=Aquifex aeolicus GN=trpB2 PE=3 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 2.537e-59
Identity = 108/172 (62.79%), Postives = 133/172 (77.33%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLN---KKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGLSFPF+ + L    +K +  + AVEP ACP+LTKG Y YDFGD+ GLTPL+KM+TLGHDFVP PIHAGGLRYHG APL+  +Y LG ++A++  QTE F+ A+ FAR+EG++PAPE  HAI A I EAL C+ETGE KVIL  + GHG+FDL AY+KYL G + D
Sbjct:  263 GGSNFAGLSFPFLADVLRGDKRKEDLKVLAVEPEACPTLTKGEYKYDFGDSVGLTPLIKMYTLGHDFVPSPIHAGGLRYHGDAPLVCKLYNLGYIDAVAYKQTEVFEAAVTFARTEGIVPAPESAHAIKAAIDEALKCKETGEEKVILFNLSGHGYFDLSAYDKYLHGELTD 434

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_METMA (Tryptophan synthase beta chain 2 OS=Methanosarcina mazei GN=trpB2 PE=3 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 1.761e-52
Identity = 96/167 (57.49%), Postives = 122/167 (73.05%), Query Frame = -3

Query:  154 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNM 654
            GGSN  G+   FI+++L  K N  + AVEP+ACPSLTKG Y YDFGDTA +TPL+KM+TLGH  +P  IHAGGLRYHG +P+IS +   GLMEA+S  Q E F  A+QFAR+EG++PAPE +HAI   I EAL  ++ GE KVIL  + GHGHFD+ +Y+KY  G +
Sbjct:  275 GGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGDTAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDMASYDKYFSGEL 441

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_METAC (Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans GN=trpB2 PE=3 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 2.300e-52
Identity = 96/168 (57.14%), Postives = 123/168 (73.21%), Query Frame = -3

Query:  151 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMV 654
            GGSN  G+   FI+++L  K +  + AVEP+ACPSLTKG Y YDFGDTA +TPL+KM+TLGH  VP  IHAGGLRYHG +P+IS +   GLMEA+S  Q E F  A+QFAR+EG++PAPE +HAI   I EAL  ++TGE K IL  + GHGHFD+ +Y+KY  G ++
Sbjct:  275 GGSNLGGIGLEFIKDRLEGKHSARVVAVEPSACPSLTKGEYRYDFGDTAEMTPLLKMYTLGHKHVPPAIHAGGLRYHGDSPIISKLCSEGLMEAVSYDQQEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALAAKQTGEEKTILFNLSGHGHFDMSSYDKYFSGELM 442

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_METTH (Tryptophan synthase beta chain 2 OS=Methanobacterium thermoautotrophicum GN=trpB2 PE=3 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 2.812e-50
Identity = 89/167 (53.29%), Postives = 121/167 (72.46%), Query Frame = -3

Query:  154 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNM 654
            GGSNF G  FPF+++KL+ K++    A EP +CP+LT G Y YDFGDTAG+TPL+KM+TLGHDFVP  +HAGGLRYHGM+P ++ +   G++ A ++PQ   F+  ++FA++EG++PAPE  HAI+  I EA  CRETGE K I+ +  GHG  DL  Y  YL+G +
Sbjct:  263 GGSNFGGAIFPFVKDKLDGKLDCEFIAAEPKSCPTLTAGEYRYDFGDTAGMTPLLKMYTLGHDFVPPSVHAGGLRYHGMSPQVALLVREGVINARAVPQHTIFESGVKFAKAEGVVPAPETCHAISVAIDEARKCRETGEEKTIVISFSGHGLLDLKGYGDYLEGKI 429

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_ARCFU (Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus GN=trpB2 PE=3 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.381e-49
Identity = 93/169 (55.03%), Postives = 119/169 (70.41%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGL++PF+ +  N  +   I AVEP ACP+LT G Y YDFGD AGLTPL+KM+TLGHDF+P PIHAGGLRYHG AP +  + + G+++A ++ Q   F+  + FAR+EG+IPAPE  HA+ A I EA+  RE  E KVI+    GHG  DL AY+ YL G + D
Sbjct:  268 GGSNFAGLTYPFVNDAKNGDLE--IIAVEPAACPTLTAGEYKYDFGDVAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGLLFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLDLQAYDDYLAGRLAD 434

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB_PYRHO (Tryptophan synthase beta chain OS=Pyrococcus horikoshii GN=trpB PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 3.437e-48
Identity = 89/169 (52.66%), Postives = 121/169 (71.60%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGLS+PFI++ L+ K      AVEP A P++TKG+Y YD+GD+AGLTP +KM+TLGH +   PIHAGGLRYHG+AP +S +   G+++ ++  QTE F+ A+ FA++EG++PAPE  HA+ A I +AL  +  GE  VIL  + GHG  DL  YE YL+G + D
Sbjct:  273 GGSNFAGLSYPFIKDVLDGKAEYEFIAVEPRAVPTMTKGVYTYDYGDSAGLTPKIKMYTLGHTYYVPPIHAGGLRYHGLAPTLSVLMNHGIVKPMAYHQTEVFEAAVLFAKAEGIVPAPESAHAVRAVIDKALEAKRKGEETVILFNLSGHGLLDLKGYEDYLEGKLED 441

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB_THEGJ (Tryptophan synthase beta chain OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=trpB PE=3 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.489e-48
Identity = 91/169 (53.85%), Postives = 117/169 (69.23%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGL++PF+R+ L+ K +    AVEP A PS+T+G+Y YDFGD+ G TP MKMHTLGH +   PIHAGGLRYHG+AP +S +   G+++ ++  QTE FQ A  FA++EG+IPAPE  HAI   I  AL  +E G  +VIL  + GHG  DL  YE YL G + D
Sbjct:  262 GGSNFAGLAYPFVRDVLSGKADYEFIAVEPKAAPSMTRGVYKYDFGDSGGYTPKMKMHTLGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVKPVAYHQTEVFQAAELFAKTEGIIPAPESAHAIKGVIDRALKAKEEGREEVILFNLSGHGLLDLKGYEDYLDGKLED 430

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB2_PYRAB (Tryptophan synthase beta chain 2 OS=Pyrococcus abyssi GN=trpB2 PE=3 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.489e-48
Identity = 88/169 (52.07%), Postives = 120/169 (71.01%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGL++PF+++ L+ K      AVEP A P++T+G+Y YD+GD+AGLTP +KMHTLGH +   PIHAGGLRYHG+AP +S +   G+++ I+  QTE F+ A+ FA++EG++PAPE  HA+ A I +AL  R  G+  VIL  + GHG  DL  YE YL G + D
Sbjct:  273 GGSNFAGLAYPFVKDVLDGKSEYEFIAVEPKAAPTMTRGVYTYDYGDSAGLTPKLKMHTLGHRYYVPPIHAGGLRYHGLAPTLSVLINHGIVKPIAYHQTEVFEAAVLFAKAEGIVPAPESAHAVKAVIDKALEARREGKEMVILFNLSGHGLLDLKGYEDYLDGKLED 441

[Close]

BLAST of CD859284 vs. SwissProt
Match: TRPB_THEON (Tryptophan synthase beta chain OS=Thermococcus onnurineus (strain NA1) GN=trpB PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 5.863e-48
Identity = 87/169 (51.48%), Postives = 120/169 (71.01%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAGL++PF++++L+ K +    AVEP A PS+T+G+Y YD+GD+ GLTP MKMHTLGH +   PIHAGGLRYHG+AP +S +   G++  ++  QTE F+ A  FA++EG++PAPE  HA+ A I  AL  +E G+ +VIL  + GHG  DL  YE +L G + D
Sbjct:  262 GGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRGVYTYDYGDSGGLTPKMKMHTLGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQTEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEGKEEVILFNLSGHGLLDLKGYEDFLDGKLED 430

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BLAST of CD859284 vs. SwissProt
Match: TRPB2_AERPE (Tryptophan synthase beta chain 2 OS=Aeropyrum pernix GN=trpB2 PE=3 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 2.228e-47
Identity = 89/169 (52.66%), Postives = 117/169 (69.23%), Query Frame = -3

Query:  148 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVD 654
            GGSNFAG ++PFIR +L    +    AVEP A PS+T+G+Y YD+GDTAGLTPL+KMHTLGH +   PIHAGGLRYHG+AP +S + + G++EA +  Q E F+ A  FA++EG++PAPE  HA+ A I EA+  R+ G   VI   + GHG  DL  Y +YL G + D
Sbjct:  270 GGSNFAGFTYPFIRHRLKGSSSTRFIAVEPKASPSMTRGVYTYDYGDTAGLTPLLKMHTLGHTYQVPPIHAGGLRYHGVAPTLSVLLKHGIVEARAYHQREVFRAAHMFAKAEGIVPAPESAHAVKAAIDEAIKARDEGRRVVIAFNLSGHGLLDLQGYREYLDGTLED 438

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BLAST of CD859284 vs. TAIR peptide
Match: AT5G38530.1 (| Symbols: TSBtype2 | tryptophan synthase beta type 2 | chr5:15424097-15426294 FORWARD LENGTH=506)

HSP 1 Score: 336.265 bits (861), Expect = 8.933e-93
Identity = 157/185 (84.86%), Postives = 173/185 (93.51%), Query Frame = -3

Query:  100 GGSNFAGLSFPFIREKLNKKINPVIRAVEPTACPSLTKGIYAYDFGDTAGLTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYELGLMEAISIPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREALHCRETGEAKVILTAMCGHGHFDLPAYEKYLQGNMVDLSFSEDRLKASHANIP 654
            GGSNFAGLSFPFIREKL  KINPVIRAVEP+ACPSLTKG+YAYDFGDTAGLTPLMKMHTLGHDF+PDPIHAGGLRYHGMAPLISHVYE G MEAISIPQ ECFQGAIQFAR+EG+IPAPEPTHAIAATIREAL C+ETGEAKVIL AMCGHGHFDL +Y+KYL+G +VDLSFSE++++ S + +P
Sbjct:  319 GGSNFAGLSFPFIREKLKGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKETGEAKVILMAMCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESLSKVP 503

The following BLAST results are available for this feature:

BLAST of CD859284 vs. TrEMBL
BLAST of CD859284 vs. SwissProt
BLAST of CD859284 vs. TAIR peptide

BLAST of CD859284 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SMI3_VITVI	3.412e-96	89.73	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9N1Y3_POPTR	2.989e-92	85.95	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q9FFW8_ARATH	1.256e-90	84.86	Tryptophan synthase beta chain OS=Arabidopsis thal...	[more]
D7MJX6_ARALY	2.798e-90	84.32	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A9NUV8_PICSI	1.030e-84	77.54	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9S4R5_PHYPA	1.756e-84	77.30	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
C5Z690_SORBI	5.650e-83	78.38	Putative uncharacterized protein Sb10g024680 OS=So...	[more]
Q67VM2_ORYSJ	6.907e-81	75.68	Putative Tryptophan synthase beta chain OS=Oryza s...	[more]
Q67VM1_ORYSJ	6.907e-81	75.68	Putative Tryptophan synthase beta chain OS=Oryza s...	[more]
A3BDS5_ORYSJ	6.907e-81	75.68	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of CD859284 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TRPB2_AQUAE	2.537e-59	62.79	Tryptophan synthase beta chain 2 OS=Aquifex aeolic...	[more]
TRPB2_METMA	1.761e-52	57.49	Tryptophan synthase beta chain 2 OS=Methanosarcina...	[more]
TRPB2_METAC	2.300e-52	57.14	Tryptophan synthase beta chain 2 OS=Methanosarcina...	[more]
TRPB2_METTH	2.812e-50	53.29	Tryptophan synthase beta chain 2 OS=Methanobacteri...	[more]
TRPB2_ARCFU	2.381e-49	55.03	Tryptophan synthase beta chain 2 OS=Archaeoglobus ...	[more]
TRPB_PYRHO	3.437e-48	52.66	Tryptophan synthase beta chain OS=Pyrococcus horik...	[more]
TRPB_THEGJ	4.489e-48	53.85	Tryptophan synthase beta chain OS=Thermococcus gam...	[more]
TRPB2_PYRAB	4.489e-48	52.07	Tryptophan synthase beta chain 2 OS=Pyrococcus aby...	[more]
TRPB_THEON	5.863e-48	51.48	Tryptophan synthase beta chain OS=Thermococcus onn...	[more]
TRPB2_AERPE	2.228e-47	52.66	Tryptophan synthase beta chain 2 OS=Aeropyrum pern...	[more]

BLAST of CD859284 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G38530.1	8.933e-93	84.86	\| Symbols: TSBtype2 \| tryptophan synthase beta typ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	PFAM	PF00291	PALP	coord: 1..149 score: 7.2
IPR001926	Pyridoxal phosphate-dependent enzyme, beta subunit	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 1..163 score: 6.4
None	No IPR available	GENE3D	3.40.50.1100	no description	coord: 1..167 score: 3.9
None	No IPR available	PANTHER	PTHR10314	SER/THR DEHYDRATASE, TRP SYNTHASE	coord: 1..178 score: 4.9
None	No IPR available	PANTHER	PTHR10314:SF3	TRYPTOPHAN SYNTHASE BETA CHAIN	coord: 1..178 score: 4.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859284 ID=CD859284; Name=CD859284; organism=Pisum sativum; type=EST; length=656bp
TTTAAACCAAAGTTCAACAACAGCCACCTACATAGTTTTACACAATATGC
TTTAAAAAGAGTGCTAAAGGACTTTTTTTCTCTTTTGTTACTAGAATGAT
GGAATATTGGCATGTGAAGCTTTCAGCCTGTCCTCTGAGAAGGAGAGATC
AACCATATTACCCTGCAAATACTTTTCATAAGCTGGCAAATCAAAATGGC
CATGTCCACACATTGCTGTCAAAATAACTTTGGCTTCTCCAGTCTCTCTA
CAATGAAGAGCTTCCCTAATGGTTGCAGCAATAGCATGAGTTGGTTCAGG
TGCTGGTATCAACCCTTCAGACCTTGCAAACTGTATAGCACCTTGAAAGC
ATTCAGTTTGCGGAATTGAAATCGCTTCCATTAAACCTAACTCATAAACA
TGAGAGATCAATGGTGCCATACCATGATAGCGCAACCCCCCAGCATGAAT
TGGATCAGGAACAAAGTCATGACCAAGTGTATGCATTTTCATCAATGGAG
TCAACCCTGCTGTATCACCAAAATCATAAGCATATATCCCTTTTGTTAAT
GAAGGACATGCTGTAGGTTCAACTGCTCTAATCACAGGATTAATTTTCTT
ATTAAGCTTCTCTCTAATGAAAGGAAAACTAAGTCCTGCAAAATTGGAAC
CACCAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001926	TrpB-like_PALP

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