CD859378

EST Overview


Name	CD859378
Unique Name	CD859378
Type	EST
Organism	Pisum sativum (pea)
Sequence length	261

Libraries


Library Name	Type
LIBEST_014000 F15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859378 vs. TrEMBL
Match: B7FN83_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.300e-32
Identity = 65/75 (86.67%), Postives = 71/75 (94.67%), Query Frame = 2

Query:   35 MGLKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            M LKDTFY+SHGSPTLSID+SI ARKFLQSWKK+VF E+P SIL+ISGHWDTTVPTVNVIQTTNDTI+DFYGFPK
Sbjct:    1 MALKDTFYISHGSPTLSIDDSIEARKFLQSWKKDVFEERPKSILVISGHWDTTVPTVNVIQTTNDTIHDFYGFPK 75

[Close]

BLAST of CD859378 vs. TrEMBL
Match: C6TJ92_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.075e-30
Identity = 60/75 (80.00%), Postives = 69/75 (92.00%), Query Frame = 2

Query:   35 MGLKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            M LKDTFY+SHGSPTLSIDESI ARKFLQSWKK+VF ++P+SIL+ISGHW+T VPTVNV+ + NDTIYDFYGFPK
Sbjct:    1 MALKDTFYISHGSPTLSIDESIQARKFLQSWKKDVFPQRPSSILVISGHWETAVPTVNVVDSINDTIYDFYGFPK 75

[Close]

BLAST of CD859378 vs. TrEMBL
Match: D7TB33_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_130.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00000251001 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.103e-23
Identity = 51/71 (71.83%), Postives = 61/71 (85.92%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DTFY+SHGSPTLSIDES+ AR FL+SW+++V G+ P SIL+ISGHW+T  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTFYISHGSPTLSIDESLPARHFLKSWREKVMGQPPTSILVISGHWETHEPTVNVV-PRNDTIYDFYGFPK 71

[Close]

BLAST of CD859378 vs. TrEMBL
Match: A5BJY7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013290 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.103e-23
Identity = 51/71 (71.83%), Postives = 61/71 (85.92%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DTFY+SHGSPTLSIDES+ AR FL+SW+++V G+ P SIL+ISGHW+T  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTFYISHGSPTLSIDESLPARHFLKSWREKVMGQPPTSILVISGHWETHEPTVNVV-PRNDTIYDFYGFPK 71

[Close]

BLAST of CD859378 vs. TrEMBL
Match: A5BGZ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021798 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.103e-23
Identity = 51/71 (71.83%), Postives = 61/71 (85.92%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DTFY+SHGSPTLSIDES+ AR FL+SWK++V G++P SIL+IS HW+T  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTFYISHGSPTLSIDESLPARHFLKSWKEKVMGQRPTSILVISAHWETHEPTVNVV-PQNDTIYDFYGFPK 71

[Close]

BLAST of CD859378 vs. TrEMBL
Match: A9PEK6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831223 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.209e-23
Identity = 49/75 (65.33%), Postives = 62/75 (82.67%), Query Frame = 2

Query:   35 MGLKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            M L +TFY+SHG+PTL+IDE++ ARKF QSW++  + EKP+SIL+IS HW+T  PTVNV+   NDTIYDFYGFPK
Sbjct:    1 MALMETFYLSHGAPTLAIDETVPARKFFQSWQQSAYKEKPSSILVISAHWETAQPTVNVVD-RNDTIYDFYGFPK 74

[Close]

BLAST of CD859378 vs. TrEMBL
Match: B9H432_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_818033 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.904e-22
Identity = 48/71 (67.61%), Postives = 59/71 (83.10%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DT Y+SHGSP ++IDESI AR+FL+SW++ V  E+P +IL+ISGHWDT  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTVYISHGSPMMAIDESIPARQFLKSWQQTVLKERPKAILVISGHWDTKEPTVNVV-NINDTIYDFYGFPK 71

[Close]

BLAST of CD859378 vs. TrEMBL
Match: B9NCP2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_590946 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.300e-21
Identity = 48/71 (67.61%), Postives = 60/71 (84.51%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DT Y+SHGSP ++IDESI ARKFL++W +++F E+P +IL+ISGHWDT  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTVYISHGSPMMAIDESIPARKFLKTW-QQIFKERPKAILVISGHWDTKEPTVNVV-NRNDTIYDFYGFPK 70

[Close]

BLAST of CD859378 vs. TrEMBL
Match: B9IP00_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_573569 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.300e-21
Identity = 48/71 (67.61%), Postives = 60/71 (84.51%), Query Frame = 2

Query:   47 DTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            DT Y+SHGSP ++IDESI ARKFL++W +++F E+P +IL+ISGHWDT  PTVNV+   NDTIYDFYGFPK
Sbjct:    2 DTVYISHGSPMMAIDESIPARKFLKTW-QQIFKERPKAILVISGHWDTKEPTVNVV-NRNDTIYDFYGFPK 70

[Close]

BLAST of CD859378 vs. TrEMBL
Match: Q6L3J4_SOLDE (4,5-DOPA dioxygenase extradiol-like protein, putative OS=Solanum demissum GN=SDM1_28t00019 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 8.739e-21
Identity = 47/74 (63.51%), Postives = 62/74 (83.78%), Query Frame = 2

Query:   41 LKDTFYVSHGSPTLSIDESIVARKFLQSWKKE-VFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFPK 259
            +K+TF++SHGSPTLSIDES+ AR FL+S+K+  +  +KPNSIL+IS HW+T+ PTVN I+   DTI+DFYGFPK
Sbjct:   40 VKETFFISHGSPTLSIDESLPARNFLKSFKERFMINQKPNSILVISAHWETSEPTVNSIRGRQDTIHDFYGFPK 113

[Close]

BLAST of CD859378 vs. SwissProt
Match: DIOXL_ARATH (4,5-DOPA dioxygenase extradiol-like protein OS=Arabidopsis thaliana GN=At4g15093 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.684e-19
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = 2

Query:   41 LKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFP 256
            +  TF++SHGSPTLSID+S+ AR+F +SW ++V  +KP SIL+IS HWDT  P+VN +   N+TI+DF GFP
Sbjct:    4 VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTV-LRNNTIHDFSGFP 74

[Close]

BLAST of CD859378 vs. SwissProt
Match: DODA_PORGR (4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora GN=DODA PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.314e-15
Identity = 37/75 (49.33%), Postives = 50/75 (66.67%), Query Frame = 2

Query:   32 KMGLKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFP 256
            ++  K++F++SHG+P +  DES +AR FL  WKK VF  KP SIL++S HW+T VP V+  Q  N  IYDF   P
Sbjct:    6 EVSFKESFFLSHGNPAMLADESFIARNFLLGWKKNVFPVKPKSILVVSAHWETDVPCVSAGQYPN-VIYDFTEVP 79

[Close]

BLAST of CD859378 vs. TAIR peptide
Match: AT4G15093.1 (| Symbols: | catalytic LigB subunit of aromatic ring-opening dioxygenase family | chr4:8618454-8619472 FORWARD LENGTH=269)

HSP 1 Score: 92.4337 bits (228), Expect = 6.006e-20
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = 2

Query:   41 LKDTFYVSHGSPTLSIDESIVARKFLQSWKKEVFGEKPNSILIISGHWDTTVPTVNVIQTTNDTIYDFYGFP 256
            +  TF++SHGSPTLSID+S+ AR+F +SW ++V  +KP SIL+IS HWDT  P+VN +   N+TI+DF GFP
Sbjct:    4 VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTV-LRNNTIHDFSGFP 74

The following BLAST results are available for this feature:

BLAST of CD859378 vs. TrEMBL
BLAST of CD859378 vs. SwissProt
BLAST of CD859378 vs. TAIR peptide

BLAST of CD859378 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FN83_MEDTR	1.300e-32	86.67	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TJ92_SOYBN	2.075e-30	80.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TB33_VITVI	1.103e-23	71.83	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BJY7_VITVI	1.103e-23	71.83	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A5BGZ7_VITVI	1.103e-23	71.83	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A9PEK6_POPTR	3.209e-23	65.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H432_POPTR	7.904e-22	67.61	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9NCP2_POPTR	2.300e-21	67.61	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IP00_POPTR	2.300e-21	67.61	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6L3J4_SOLDE	8.739e-21	63.51	4,5-DOPA dioxygenase extradiol-like protein, putat...	[more]

BLAST of CD859378 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
DIOXL_ARATH	7.684e-19	56.94	4,5-DOPA dioxygenase extradiol-like protein OS=Ara...	[more]
DODA_PORGR	2.314e-15	49.33	4,5-DOPA dioxygenase extradiol OS=Portulaca grandi...	[more]

BLAST of CD859378 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G15093.1	6.006e-20	56.94	\| Symbols: \| catalytic LigB subunit of aromatic r...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	PFAM	PF02900	LigB	coord: 9..78 score: 7.3
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	SUPERFAMILY	53213	LigB subunit of an aromatic-ring-opening dioxygenase LigAB	coord: 4..78 score: 8.2
None	No IPR available	GENE3D	3.40.830.10	no description	coord: 3..78 score: 2.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859378 ID=CD859378; Name=CD859378; organism=Pisum sativum; type=EST; length=261bp
GCGATAGAGAGAAGCAATTAATTAATCAACAAAAATGGGTTTGAAGGATA
CGTTTTACGTCTCACATGGATCACCAACGTTGTCGATAGATGAATCGATT
GTGGCGAGGAAGTTTCTACAATCATGGAAGAAGGAAGTGTTTGGGGAGAA
ACCTAATTCGATTTTGATTATTTCTGGTCATTGGGATACGACTGTTCCGA
CCGTTAACGTTATTCAGACAACCAATGATACAATCTATGACTTCTATGGT
TTCCCCAAACC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006725	cellular aromatic compound metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008198	ferrous iron binding
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR004183	Xdiol_dOase_suB

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