CD859868

EST Overview


Name	CD859868
Unique Name	CD859868
Type	EST
Organism	Pisum sativum (pea)
Sequence length	771

Libraries


Library Name	Type
LIBEST_014001 G15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859868 vs. TrEMBL
Match: O24294_PEA (Legumin (Minor small) OS=Pisum sativum GN=legS PE=2 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.658e-72
Identity = 151/229 (65.94%), Postives = 152/229 (66.38%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPRSYETRRKWKKHTAEKKRESHGQGXXXXXXXXXXXXXXXIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPEL                                                           PRSYETRRKWKKHTAEK+RESHGQG               IQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAE  LLYRNGIYAPHWNI ANSL
Sbjct:  232 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPELQEEEEQSHSQRKEEEEEEQEQRHRKHSKKEDEDEDEEEEEEREQRHRKHSEKEEEDEDEPRSYETRRKWKKHTAEKERESHGQGEEEEELEKEEEEEEGIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEYVLLYRNGIYAPHWNINANSL 460

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q43673_VICFA (Legumin; legumin-related high molecular weight polypeptide OS=Vicia faba var. minor GN=LelB3 PE=3 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 7.718e-62
Identity = 136/229 (59.39%), Postives = 143/229 (62.45%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPRSYETRRKWKKHTAEKKRESHGQGXXXXXXXXXXXXXXXIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            VLSGF+ EFL HSLNTKEDTAKRLRSP+D+RGQIVKVE+GL IIS EL                                                           PRSYE+R KWKKHT EKKRESHG+G               IQRQHSKG KNGLEETICS+KIRENIARPSR DLYNS AGRISTVNSLTLPILRNLRLSAE  LLYRNGIYAPHWNI ANSL
Sbjct:  227 VLSGFSAEFLGHSLNTKEDTAKRLRSPRDQRGQIVKVENGLDIISAELQEEEQQSHSQGEEEEEEEREQQRHRERYSEKEDEDEDEKEEQEQRHGKHSKKEEEDEDEPRSYESR-KWKKHTEEKKRESHGRGEEKQEQEKKEEEEEEIQRQHSKGGKNGLEETICSSKIRENIARPSRADLYNSRAGRISTVNSLTLPILRNLRLSAEYVLLYRNGIYAPHWNINANSL 454

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q41703_VICSA (Legumin B OS=Vicia sativa PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 8.061e-27
Identity = 62/81 (76.54%), Postives = 67/81 (82.72%), Query Frame = 3

Query:  516 QRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++  S+  +NGLEETICSAKIRENIA P+R DLYN  AGRIST NSLTLPILR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  295 EKHRSEQGRNGLEETICSAKIRENIANPARADLYNPRAGRISTANSLTLPILRYLRLSAEYVRLYRNGIYAPHWNIDANSL 375

HSP 2 Score: 70.0922 bits (170), Expect = 2.356e-10
Identity = 34/47 (72.34%), Postives = 40/47 (85.11%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLA + NT+ED AKRLRSP+D+R QIVKVE GL II+PE
Sbjct:  237 VLSGFSSEFLAQTFNTEEDIAKRLRSPRDQRNQIVKVEGGLRIINPE 283

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q9SB12_SOYBN (Glycinin OS=Glycine max PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q7GC77_SOYBN (Glycinin A3B4 subunit OS=Glycine max PE=1 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q39922_GLYSO (Gy5 protein OS=Glycine soja GN=Gy5 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. TrEMBL
Match: Q39858_SOYBN (Soybean glycinin A3-B4 subunit (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:   54 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYLVLYRNGIYSPHWNLNANSV 138

[Close]

BLAST of CD859868 vs. TrEMBL
Match: P93708_SOYBN (Glycinin OS=Glycine max GN=Gly A3B4 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. TrEMBL
Match: P93707_SOYBN (Glycinin OS=Glycine max GN=Gly A3B4 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. TrEMBL
Match: C0KG62_SOYBN (Mutant glycinin A3B4 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.684e-22
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+PHWN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPHWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 7.580e-9
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGB_PEA (Legumin B (Fragment) OS=Pisum sativum GN=LEGB PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.719e-74
Identity = 152/229 (66.38%), Postives = 152/229 (66.38%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPELXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPRSYETRRKWKKHTAEKKRESHGQGXXXXXXXXXXXXXXXIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPEL                                                           PRSYETRRKWKKHTAEKKRESHGQG               IQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAE  LLYRNGIYAPHWNI ANSL
Sbjct:    4 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPELQEEEEQSHSQRKEEEEEEQEQRHRKHSKKEDEDEDEEEEEEREQRHRKHSEKEEEDEDEPRSYETRRKWKKHTAEKKRESHGQGEEEEELEKEEEEEEEIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEYVLLYRNGIYAPHWNINANSL 232

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGJ_PEA (Legumin J OS=Pisum sativum GN=LEGJ PE=1 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.636e-29
Identity = 69/114 (60.53%), Postives = 76/114 (66.67%), Query Frame = 3

Query:  417 KWKKHTAEKKRESHGQGXXXXXXXXXXXXXXXIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            K K    E+K +SH                   ++Q S+ RKNGLEETICSAKIRENIA  +R DLYN  AGRIST NSLTLP+LR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  280 KPKGKEEEEKEQSHSHSHREEKEEEEEEEEDEEEKQRSEERKNGLEETICSAKIRENIADAARADLYNPRAGRISTANSLTLPVLRYLRLSAEYVRLYRNGIYAPHWNINANSL 393

HSP 2 Score: 70.4774 bits (171), Expect = 1.178e-11
Identity = 34/47 (72.34%), Postives = 40/47 (85.11%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLA + NT+EDTAKRLRSP+DER QIV+VE GL II P+
Sbjct:  236 VLSGFSSEFLAQTFNTEEDTAKRLRSPRDERSQIVRVEGGLRIIKPK 282

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGB7_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB7 PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.531e-27
Identity = 61/81 (75.31%), Postives = 67/81 (82.72%), Query Frame = 3

Query:  516 QRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q S+  +NGLEETICS KIRENIA+P+R DLYN  AG IST NSLTLPILR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  145 EKQRSEQGRNGLEETICSLKIRENIAQPARADLYNPRAGSISTANSLTLPILRYLRLSAEYVRLYRNGIYAPHWNINANSL 225

HSP 2 Score: 70.8626 bits (172), Expect = 9.017e-12
Identity = 34/47 (72.34%), Postives = 41/47 (87.23%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLA + NT+EDTAKRLRSP+D+R QIV+VE GL II+PE
Sbjct:   87 VLSGFSSEFLAQTFNTEEDTAKRLRSPRDKRNQIVRVEGGLRIINPE 133

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGB6_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB6 PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.531e-27
Identity = 61/81 (75.31%), Postives = 67/81 (82.72%), Query Frame = 3

Query:  516 QRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q S+  +NGLEETICS KIRENIA+P+R DLYN  AG IST NSLTLPILR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  139 EKQRSEQGRNGLEETICSLKIRENIAQPARADLYNPRAGSISTANSLTLPILRYLRLSAEYVRLYRNGIYAPHWNINANSL 219

HSP 2 Score: 70.8626 bits (172), Expect = 9.017e-12
Identity = 34/47 (72.34%), Postives = 41/47 (87.23%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLA + NT+EDTAKRLRSP+D+R QIV+VE GL II+PE
Sbjct:   81 VLSGFSSEFLAQTFNTEEDTAKRLRSPRDKRNQIVRVEGGLRIINPE 127

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGB4_VICFA (Legumin type B OS=Vicia faba GN=LEB4 PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.531e-27
Identity = 61/81 (75.31%), Postives = 67/81 (82.72%), Query Frame = 3

Query:  516 QRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q S+  +NGLEETICS KIRENIA+P+R DLYN  AG IST NSLTLPILR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  294 EKQRSEQGRNGLEETICSLKIRENIAQPARADLYNPRAGSISTANSLTLPILRYLRLSAEYVRLYRNGIYAPHWNINANSL 374

HSP 2 Score: 73.9442 bits (180), Expect = 1.065e-12
Identity = 35/47 (74.47%), Postives = 42/47 (89.36%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLAH+ NT+EDTAKRLRSP+D+R QIV+VE GL II+PE
Sbjct:  236 VLSGFSSEFLAHTFNTEEDTAKRLRSPRDKRNQIVRVEGGLRIINPE 282

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGB2_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB2 PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.531e-27
Identity = 61/81 (75.31%), Postives = 67/81 (82.72%), Query Frame = 3

Query:  516 QRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q S+  +NGLEETICS KIRENIA+P+R DLYN  AG IST NSLTLPILR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  145 EKQRSEQGRNGLEETICSLKIRENIAQPARADLYNPRAGSISTANSLTLPILRYLRLSAEYVRLYRNGIYAPHWNINANSL 225

HSP 2 Score: 70.8626 bits (172), Expect = 9.017e-12
Identity = 34/47 (72.34%), Postives = 41/47 (87.23%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+ EFLA + NT+EDTAKRLRSP+D+R QIV+VE GL II+PE
Sbjct:   87 VLSGFSSEFLAQTFNTEEDTAKRLRSPRDKRNQIVRVEGGLRIINPE 133

[Close]

BLAST of CD859868 vs. SwissProt
Match: LEGK_PEA (Legumin K (Fragment) OS=Pisum sativum GN=LEGK PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.611e-27
Identity = 66/111 (59.46%), Postives = 73/111 (65.77%), Query Frame = 3

Query:  426 KHTAEKKRESHGQGXXXXXXXXXXXXXXXIQRQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            K   E+K +SH                   ++Q S+ RKNGLEETICSAKIRENIA  +  DLYN  AGRI T NSLTLP+LR LRLSAE   LYRNGIYAPHWNI ANSL
Sbjct:  134 KEEEEEKEQSHSHSHREEEEEEEEDE----EKQRSEERKNGLEETICSAKIRENIADAAGADLYNPRAGRIRTANSLTLPVLRYLRLSAEYVRLYRNGIYAPHWNINANSL 240

HSP 2 Score: 68.1662 bits (165), Expect = 5.845e-11
Identity = 33/47 (70.21%), Postives = 40/47 (85.11%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSG + EFLA + NT+EDTAKRLRSP+DER QIV+VE GL II+P+
Sbjct:   86 VLSGVSSEFLAQTFNTEEDTAKRLRSPRDERSQIVRVEGGLRIINPK 132

[Close]

BLAST of CD859868 vs. SwissProt
Match: GLYG4_SOYBN (Glycinin G4 OS=Glycine max GN=GY4 PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 5.096e-23
Identity = 49/80 (61.25%), Postives = 62/80 (77.50%), Query Frame = 3

Query:  519 RQHSKGRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            R+     +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR  +LSA+  +LY+NGIY+PHWN+ ANS+
Sbjct:  369 RERGCETRNGVEENICTLKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFQLSAQYVVLYKNGIYSPHWNLNANSV 448

HSP 2 Score: 60.8474 bits (146), Expect = 9.331e-9
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT ED A++L SP DER QIV VE GL +ISP+
Sbjct:  229 VLSGFSKHFLAQSFNTNEDIAEKLESPDDERKQIVTVEGGLSVISPK 275

[Close]

BLAST of CD859868 vs. SwissProt
Match: GLYG5_SOYBN (Glycinin OS=Glycine max PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.937e-22
Identity = 51/85 (60.00%), Postives = 64/85 (75.29%), Query Frame = 3

Query:  516 QRQHSKGR----KNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            ++Q  +GR    +NG+EE IC+ K+ ENIARPSR D YN  AGRIST+NSLTLP LR   LSA+  +LYRNGIY+P WN+ ANS+
Sbjct:  331 EQQEPRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLSAQYVVLYRNGIYSPDWNLNANSV 415

HSP 2 Score: 65.0846 bits (157), Expect = 4.948e-10
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 3

Query:   72 VLSGFNVEFLAHSLNTKEDTAKRLRSPQDERGQIVKVEDGLHIISPE 212
            VLSGF+  FLA S NT EDTA++LRSP DER QIV VE GL +ISP+
Sbjct:  228 VLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPK 274

[Close]

BLAST of CD859868 vs. SwissProt
Match: 13SB_FAGES (13S globulin basic chain OS=Fagopyrum esculentum PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.100e-17
Identity = 41/70 (58.57%), Postives = 51/70 (72.86%), Query Frame = 3

Query:  546 GLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANS 755
            G++E +C+ K+RENI  P   D YN  AGRI+T NS  LP LR+L++SAE   LY NGIYAPHWNI A+S
Sbjct:    1 GIDENVCTMKLRENIKSPQEADFYNPKAGRITTANSQKLPALRSLQMSAERGFLYSNGIYAPHWNINAHS 70

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT1G03890.1 (| Symbols: | RmlC-like cupins superfamily protein | chr1:989250-990908 FORWARD LENGTH=451)

HSP 1 Score: 77.411 bits (189), Expect = 9.625e-15
Identity = 38/75 (50.67%), Postives = 51/75 (68.00%), Query Frame = 3

Query:  534 GRKNGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            G  NG+EET C+AKI ENI  P R D +++ AGRIST+NSL LP+LR +RL+A    LY  G+  P W   A+++
Sbjct:  267 GIANGIEETYCTAKIHENIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAHTV 341

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT5G44120.2 (| Symbols: CRA1, ATCRA1, CRU1 | RmlC-like cupins superfamily protein | chr5:17756460-17757811 REVERSE LENGTH=368)

HSP 1 Score: 74.3294 bits (181), Expect = 8.148e-14
Identity = 37/72 (51.39%), Postives = 48/72 (66.67%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            NGLEETICSA+  +N+  PSR D+Y    G IST+NS  LPILR +RLSA    + +N +  P WN  AN++
Sbjct:  178 NGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAI 249

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT5G44120.1 (| Symbols: CRA1, ATCRA1, CRU1 | RmlC-like cupins superfamily protein | chr5:17756460-17757432 REVERSE LENGTH=285)

HSP 1 Score: 74.3294 bits (181), Expect = 8.148e-14
Identity = 37/72 (51.39%), Postives = 48/72 (66.67%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            NGLEETICSA+  +N+  PSR D+Y    G IST+NS  LPILR +RLSA    + +N +  P WN  AN++
Sbjct:   95 NGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAI 166

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT5G44120.3 (| Symbols: CRA1, ATCRA1, CRU1 | RmlC-like cupins superfamily protein | chr5:17756460-17758246 REVERSE LENGTH=472)

HSP 1 Score: 74.3294 bits (181), Expect = 8.148e-14
Identity = 37/72 (51.39%), Postives = 48/72 (66.67%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL 758
            NGLEETICSA+  +N+  PSR D+Y    G IST+NS  LPILR +RLSA    + +N +  P WN  AN++
Sbjct:  282 NGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAI 353

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT4G28520.1 (| Symbols: CRU3, CRC | cruciferin 3 | chr4:14087596-14089617 FORWARD LENGTH=524)

HSP 1 Score: 73.9442 bits (180), Expect = 1.064e-13
Identity = 38/76 (50.00%), Postives = 50/76 (65.79%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANSL--CT 764
            NGLEETICS +  ENI  P+R D+Y    GR+++VNS TLPIL  +RLSA   +L  N +  P +N+ AN +  CT
Sbjct:  333 NGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSATRGVLQGNAMVLPKYNMNANEILYCT 408

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT1G03880.1 (| Symbols: CRU2, CRB | cruciferin 2 | chr1:985786-987916 FORWARD LENGTH=455)

HSP 1 Score: 72.0182 bits (175), Expect = 4.044e-13
Identity = 35/71 (49.30%), Postives = 46/71 (64.79%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSAEICLLYRNGIYAPHWNIKANS 755
            NGLEET+C+ +  EN+  PS  D+Y    G IST+NS  LPILR LRLSA    + +N +  P WN+ AN+
Sbjct:  269 NGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNVNANA 339

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT4G28520.4 (| Symbols: CRU3, CRC | cruciferin 3 | chr4:14087596-14089229 FORWARD LENGTH=396)

HSP 1 Score: 63.1586 bits (152), Expect = 1.878e-10
Identity = 30/50 (60.00%), Postives = 37/50 (74.00%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSA 692
            NGLEETICS +  ENI  P+R D+Y    GR+++VNS TLPIL  +RLSA
Sbjct:  333 NGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSA 382

[Close]

BLAST of CD859868 vs. TAIR peptide
Match: AT4G28520.2 (| Symbols: CRU3, CRC | cruciferin 3 | chr4:14087596-14089229 FORWARD LENGTH=394)

HSP 1 Score: 63.1586 bits (152), Expect = 1.878e-10
Identity = 30/50 (60.00%), Postives = 37/50 (74.00%), Query Frame = 3

Query:  543 NGLEETICSAKIRENIARPSRGDLYNSGAGRISTVNSLTLPILRNLRLSA 692
            NGLEETICS +  ENI  P+R D+Y    GR+++VNS TLPIL  +RLSA
Sbjct:  333 NGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSA 382

The following BLAST results are available for this feature:

BLAST of CD859868 vs. TrEMBL
BLAST of CD859868 vs. SwissProt
BLAST of CD859868 vs. TAIR peptide

BLAST of CD859868 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O24294_PEA	1.658e-72	65.94	Legumin (Minor small) OS=Pisum sativum GN=legS PE=...	[more]
Q43673_VICFA	7.718e-62	59.39	Legumin; legumin-related high molecular weight pol...	[more]
Q41703_VICSA	8.061e-27	76.54	Legumin B OS=Vicia sativa PE=2 SV=1	[more]
Q9SB12_SOYBN	2.684e-22	61.18	Glycinin OS=Glycine max PE=3 SV=1	[more]
Q7GC77_SOYBN	2.684e-22	61.18	Glycinin A3B4 subunit OS=Glycine max PE=1 SV=1	[more]
Q39922_GLYSO	2.684e-22	61.18	Gy5 protein OS=Glycine soja GN=Gy5 PE=2 SV=1	[more]
Q39858_SOYBN	2.684e-22	61.18	Soybean glycinin A3-B4 subunit (Fragment) OS=Glyci...	[more]
P93708_SOYBN	2.684e-22	61.18	Glycinin OS=Glycine max GN=Gly A3B4 PE=2 SV=1	[more]
P93707_SOYBN	2.684e-22	61.18	Glycinin OS=Glycine max GN=Gly A3B4 PE=2 SV=1	[more]
C0KG62_SOYBN	2.684e-22	61.18	Mutant glycinin A3B4 OS=Glycine max PE=2 SV=1	[more]

BLAST of CD859868 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LEGB_PEA	3.719e-74	66.38	Legumin B (Fragment) OS=Pisum sativum GN=LEGB PE=2...	[more]
LEGJ_PEA	1.636e-29	60.53	Legumin J OS=Pisum sativum GN=LEGJ PE=1 SV=1	[more]
LEGB7_VICFA	1.531e-27	75.31	Legumin type B (Fragment) OS=Vicia faba GN=LEB7 PE...	[more]
LEGB6_VICFA	1.531e-27	75.31	Legumin type B (Fragment) OS=Vicia faba GN=LEB6 PE...	[more]
LEGB4_VICFA	1.531e-27	75.31	Legumin type B OS=Vicia faba GN=LEB4 PE=3 SV=1	[more]
LEGB2_VICFA	1.531e-27	75.31	Legumin type B (Fragment) OS=Vicia faba GN=LEB2 PE...	[more]
LEGK_PEA	2.611e-27	59.46	Legumin K (Fragment) OS=Pisum sativum GN=LEGK PE=3...	[more]
GLYG4_SOYBN	5.096e-23	61.25	Glycinin G4 OS=Glycine max GN=GY4 PE=1 SV=1	[more]
GLYG5_SOYBN	1.937e-22	60.00	Glycinin OS=Glycine max PE=1 SV=1	[more]
13SB_FAGES	1.100e-17	58.57	13S globulin basic chain OS=Fagopyrum esculentum P...	[more]

BLAST of CD859868 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 8


Match Name	E-value	Identity	Description
AT1G03890.1	9.625e-15	50.67	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT5G44120.2	8.148e-14	51.39	\| Symbols: CRA1, ATCRA1, CRU1 \| RmlC-like cupins s...	[more]
AT5G44120.1	8.148e-14	51.39	\| Symbols: CRA1, ATCRA1, CRU1 \| RmlC-like cupins s...	[more]
AT5G44120.3	8.148e-14	51.39	\| Symbols: CRA1, ATCRA1, CRU1 \| RmlC-like cupins s...	[more]
AT4G28520.1	1.064e-13	50.00	\| Symbols: CRU3, CRC \| cruciferin 3 \| chr4:1408759...	[more]
AT1G03880.1	4.044e-13	49.30	\| Symbols: CRU2, CRB \| cruciferin 2 \| chr1:985786-...	[more]
AT4G28520.4	1.878e-10	60.00	\| Symbols: CRU3, CRC \| cruciferin 3 \| chr4:1408759...	[more]
AT4G28520.2	1.878e-10	60.00	\| Symbols: CRU3, CRC \| cruciferin 3 \| chr4:1408759...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 12..32 score: NAcoord: 39..51 score: NAcoord: 61..76 score
None	No IPR available	SEG	seg	seg	coord: 1..9 score
None	No IPR available	SEG	seg	seg	coord: 8..40 score
None	No IPR available	SEG	seg	seg	coord: 4..23 score: NAcoord: 72..130 score: NAcoord: 156..170 score
None	No IPR available	SEG	seg	seg	coord: 6..44 score: NAcoord: 70..87 score: NAcoord: 93..105 score
None	No IPR available	SEG	seg	seg	coord: 4..51 score
None	No IPR available	SEG	seg	seg	coord: 9..19 score: NAcoord: 25..52 score
None	No IPR available	SEG	seg	seg	coord: 16..81 score
IPR006044	11-S seed storage protein, plant	PRINTS	PR00439	11SGLOBULIN	coord: 193..210 score: 2.1e-18coord: 216..236 score: 2.1e-18coord: 238..256 score: 2.1
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 195..252 score: 2.4
IPR011051	Cupin, RmlC-type	SUPERFAMILY	51182	RmlC-like cupins	coord: 10..252 score: 5.2
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	no description	coord: 5..74 score: 3.4e-15coord: 198..252 score: 3.5
IPR022379	11-S seed storage protein, conserved site	PROSITE	PS00305	11S_SEED_STORAGE	coord: 181..203 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859868 ID=CD859868; Name=CD859868; organism=Pisum sativum; type=EST; length=771bp
GTTTCTTGGTTGGACGTAGAGGTGGACAACAACAAGAGGAGGAATCTGAG
GAACAAAACGAAGGTAACAGCGTGCTGAGTGGCTTTAACGTAGAGTTTTT
AGCACATTCACTCAACACCAAAGAGGATACAGCCAAGAGACTTCGATCTC
CACAGGACGAAAGGGGTCAAATCGTAAAAGTAGAGGACGGTCTTCACATC
ATCAGTCCTGAGTTGCAAGAAGAAGAAGAACAAAGTCACAGTCAACGGAA
GGAGGAAGAGGAAGAAGAACAAGAACAAAGACACCGCAAACATAGCAAAA
AAGAAGATGAGGATGAGGATGAAGAAGAAGAAGAAGAACGAGAACAAAGA
CACCGCAAACATAGCGAAAAAGAAGAGGAGGATGAGGATGAACCTCGCAG
CTATGAGACTCGTCGAAAGTGGAAAAAACACACCGCAGAGAAGAAACGAG
AATCACACGGACAAGGGGAAGAAGAAGAAGAACTAGAAAAGGAAGAGGAG
GAAGAGGAAGAAATACAACGTCAACACAGCAAAGGAAGAAAGAATGGTTT
GGAAGAAACTATTTGCAGTGCAAAGATTCGTGAGAACATCGCTCGCCCTT
CACGTGGTGATCTCTACAACTCAGGTGCCGGTCGCATCAGCACTGTCAAC
AGTTTAACCCTCCCCATCCTCCGCAATTTACGCCTCAGTGCTGAAATATG
TCTTCTCTACAGGAATGGTATATATGCTCCACACTGGAACATCAAGGCAA
ACAGCCTCTGTACTCGGACGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0045735	nutrient reservoir activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006044	11S_seedstore_pln
IPR006045	Cupin_1
IPR011051	RmlC_Cupin_sf
IPR014710	RmlC-like_jellyroll
IPR022379	11S_seedstore_CS

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