EX569178

EST Overview
NameEX569178
Unique NameEX569178
TypeEST
OrganismPisum sativum (pea)
Sequence length473
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
KEGG analysis for Pisum sativum unigene v12011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of EX569178 vs. TrEMBL
Match: C6T4U1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 2.833e-26
Identity = 53/59 (89.83%), Postives = 55/59 (93.22%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGFL+TTLIFVV+G IACLCTRI CNRGPSA LFHLTLVITATICCWMMWAIVYLAQ K
Sbjct:    1 MGFLVTTLIFVVIGIIACLCTRIRCNRGPSANLFHLTLVITATICCWMMWAIVYLAQMK 59

HSP 2 Score: 26.1794 bits (56), Expect = 2.833e-26
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPIL+EGE
Sbjct:   59 KPLIVPILNEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: B3FNQ0_HEVBR (ATP synthase subunit H protein OS=Hevea brasiliensis PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.060e-25
Identity = 51/59 (86.44%), Postives = 54/59 (91.53%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGFL+ TLIFVV+G IA LCTRICCNRGPSA LFHLTLVITAT+CCWMMWAIVYLAQ K
Sbjct:    1 MGFLVITLIFVVIGIIASLCTRICCNRGPSANLFHLTLVITATVCCWMMWAIVYLAQMK 59

HSP 2 Score: 26.1794 bits (56), Expect = 1.060e-25
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPIL+EGE
Sbjct:   59 KPLIVPILNEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: A9P8S8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_728008 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.797e-25
Identity = 50/59 (84.75%), Postives = 53/59 (89.83%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGFL+TTLIFVVVG IA LCTRICCNRGPS  L HLTLV+TAT+CCWMMWAIVYLAQ K
Sbjct:    1 MGFLVTTLIFVVVGIIASLCTRICCNRGPSTNLLHLTLVLTATVCCWMMWAIVYLAQMK 59

HSP 2 Score: 27.335 bits (59), Expect = 1.797e-25
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPILSEGE
Sbjct:   59 KPLIVPILSEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: A9PJE6_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.930e-24
Identity = 47/59 (79.66%), Postives = 51/59 (86.44%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGF +TTLIF V+G IA LCTRICCNRGPS  L HLTLV+TAT+CCWMMWAIVYLAQ K
Sbjct:    1 MGFFVTTLIFAVIGIIASLCTRICCNRGPSTNLLHLTLVLTATVCCWMMWAIVYLAQMK 59

HSP 2 Score: 27.335 bits (59), Expect = 1.930e-24
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPILSEGE
Sbjct:   59 KPLIVPILSEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: A9P9Z7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_815748 PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.930e-24
Identity = 47/59 (79.66%), Postives = 51/59 (86.44%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGF +TTLIF V+G IA LCTRICCNRGPS  L HLTLV+TAT+CCWMMWAIVYLAQ K
Sbjct:    1 MGFFVTTLIFAVIGIIASLCTRICCNRGPSTNLLHLTLVLTATVCCWMMWAIVYLAQMK 59

HSP 2 Score: 27.335 bits (59), Expect = 1.930e-24
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPILSEGE
Sbjct:   59 KPLIVPILSEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: Q53U40_SOLLC (Similar to ATP synthase subunit H protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.258e-24
Identity = 47/59 (79.66%), Postives = 51/59 (86.44%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGFL+TTLIFV +G IA LC RICCNRGPS  L HLTL+ITAT+CCWMMWAIVYLAQ K
Sbjct:    1 MGFLVTTLIFVAIGVIASLCARICCNRGPSTNLLHLTLIITATVCCWMMWAIVYLAQLK 59

HSP 2 Score: 26.9498 bits (58), Expect = 4.258e-24
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVP+LSEGE
Sbjct:   59 KPLIVPVLSEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: D7MKG2_ARALY (ATP synthase subunit H family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_918672 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 3.510e-23
Identity = 49/57 (85.96%), Postives = 51/57 (89.47%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            MGFLITTLIFVVVG IA LC RICCNRGPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MGFLITTLIFVVVGIIAALCARICCNRGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 22.3274 bits (46), Expect = 3.510e-23
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSETE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: Q9FLN5_ARATH (AT5G55290 protein OS=Arabidopsis thaliana GN=AT5G55290 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 4.569e-23
Identity = 49/57 (85.96%), Postives = 51/57 (89.47%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            MGFLITTLIFVVVG IA LC RICCNRGPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MGFLITTLIFVVVGIIASLCVRICCNRGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 22.3274 bits (46), Expect = 4.569e-23
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSETE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: D7T851_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_90.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00037739001 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.008e-22
Identity = 45/57 (78.95%), Postives = 49/57 (85.96%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            MGF +TTLIF+V G IA  C RICCN+GPSA L HLTLVITAT+CCWMMWAIVYLAQ
Sbjct:    1 MGFFVTTLIFIVTGIIASFCARICCNKGPSANLLHLTLVITATVCCWMMWAIVYLAQ 57

HSP 2 Score: 25.409 bits (54), Expect = 1.008e-22
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSEGE
Sbjct:   60 PLIVPILSEGE 70
[Close]
BLAST of EX569178 vs. TrEMBL
Match: C6T343_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.891e-22
Identity = 45/59 (76.27%), Postives = 50/59 (84.75%), Query Frame = -1 
Query:  174 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQKK 350
            MGF +TTL+F V+G +A L TRIC NRGPSA LFHLTLV+TAT CCWMMWAIVYLAQ K
Sbjct:    1 MGFSVTTLVFAVIGIVASLSTRICFNRGPSANLFHLTLVLTATTCCWMMWAIVYLAQMK 59

HSP 2 Score: 27.335 bits (59), Expect = 2.891e-22
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = -2 
Query:  140 KPLIVPILSEGE 175
            KPLIVPILSEGE
Sbjct:   59 KPLIVPILSEGE 70
[Close]
BLAST of EX569178 vs. TAIR peptide
Match: AT5G55290.2 (| Symbols: | ATPase, V0 complex, subunit E | chr5:22423037-22424086 REVERSE LENGTH=70)

HSP 1 Score: 109.383 bits (272), Expect = 3.578e-25
Identity = 49/57 (85.96%), Postives = 51/57 (89.47%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            MGFLITTLIFVVVG IA LC RICCNRGPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MGFLITTLIFVVVGIIASLCVRICCNRGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 22.3274 bits (46), Expect = 3.578e-25
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSETE 70
[Close]
BLAST of EX569178 vs. TAIR peptide
Match: AT5G55290.1 (| Symbols: | ATPase, V0 complex, subunit E | chr5:22423037-22424086 REVERSE LENGTH=70)

HSP 1 Score: 109.383 bits (272), Expect = 3.578e-25
Identity = 49/57 (85.96%), Postives = 51/57 (89.47%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            MGFLITTLIFVVVG IA LC RICCNRGPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MGFLITTLIFVVVGIIASLCVRICCNRGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 22.3274 bits (46), Expect = 3.578e-25
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSETE 70
[Close]
BLAST of EX569178 vs. TAIR peptide
Match: AT4G26710.2 (| Symbols: | ATPase, V0 complex, subunit E | chr4:13468705-13469668 FORWARD LENGTH=70)

HSP 1 Score: 98.2117 bits (243), Expect = 9.676e-22
Identity = 42/57 (73.68%), Postives = 49/57 (85.96%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            M F++T+LIF VVG IA +CTRIC N+GPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MAFVVTSLIFAVVGIIASICTRICFNKGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 21.9422 bits (45), Expect = 9.676e-22
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSEVE 70
[Close]
BLAST of EX569178 vs. TAIR peptide
Match: AT4G26710.1 (| Symbols: | ATPase, V0 complex, subunit E | chr4:13468705-13469668 FORWARD LENGTH=70)

HSP 1 Score: 98.2117 bits (243), Expect = 9.676e-22
Identity = 42/57 (73.68%), Postives = 49/57 (85.96%), Query Frame = -1 
Query:  180 MGFLITTLIFVVVGFIACLCTRICCNRGPSAKLFHLTLVITATICCWMMWAIVYLAQ 350
            M F++T+LIF VVG IA +CTRIC N+GPS  L HLTLVITAT+CCWMMWAIVY+AQ
Sbjct:    1 MAFVVTSLIFAVVGIIASICTRICFNKGPSTNLLHLTLVITATVCCWMMWAIVYIAQ 57

HSP 2 Score: 21.9422 bits (45), Expect = 9.676e-22
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = -2 
Query:  140 PLIVPILSEGE 172
            PLIVPILSE E
Sbjct:   60 PLIVPILSEVE 70
The following BLAST results are available for this feature:
BLAST of EX569178 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6T4U1_SOYBN2.833e-2689.83Putative uncharacterized protein OS=Glycine max PE... [more]
B3FNQ0_HEVBR1.060e-2586.44ATP synthase subunit H protein OS=Hevea brasiliens... [more]
A9P8S8_POPTR1.797e-2584.75Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
A9PJE6_9ROSI1.930e-2479.66Putative uncharacterized protein OS=Populus tricho... [more]
A9P9Z7_POPTR1.930e-2479.66Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q53U40_SOLLC4.258e-2479.66Similar to ATP synthase subunit H protein OS=Solan... [more]
D7MKG2_ARALY3.510e-2385.96ATP synthase subunit H family protein OS=Arabidops... [more]
Q9FLN5_ARATH4.569e-2385.96AT5G55290 protein OS=Arabidopsis thaliana GN=AT5G5... [more]
D7T851_VITVI1.008e-2278.95Whole genome shotgun sequence of line PN40024, sca... [more]
C6T343_SOYBN2.891e-2276.27Putative uncharacterized protein OS=Glycine max PE... [more]
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BLAST of EX569178 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4
Match NameE-valueIdentityDescription
AT5G55290.23.578e-2585.96| Symbols: | ATPase, V0 complex, subunit E | chr5... [more]
AT5G55290.13.578e-2585.96| Symbols: | ATPase, V0 complex, subunit E | chr5... [more]
AT4G26710.29.676e-2273.68| Symbols: | ATPase, V0 complex, subunit E | chr4... [more]
AT4G26710.19.676e-2273.68| Symbols: | ATPase, V0 complex, subunit E | chr4... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 21..44
score
IPR008389ATPase, V0 complex, subunit EPFAMPF05493ATP_synt_Hcoord: 42..98
score: 4.9

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX569178 ID=EX569178; Name=EX569178; organism=Pisum sativum; type=EST; length=473bp
CAACACAAAAAAATACATACATATCCAACCTTAGGAAATAATCAACGACT
CATGAGAGCTGATATCAAATTCTCTCCAGCATCCAATCTGAGATCCATAT
TATCTAACATACAAGAAACTACATTTGTTCAAAAACTTATTCGCCTTCAC
TTAGTATTGGTACTATGAGTGGTTTTTTTTTGTGCCAGATATACGATCGC
CCACATCATCCAGCAGCATATTGTTGCGGTAATAACCAGAGTTAAGTGAA
ATAGTTTAGCAGAAGGTCCTCTATTGCAGCAAATTCTGGTGCAGAGACAC
GCGATGAACCCAACCACCACGAAGATTAGTGTCGTTATCAAGAAACCCAT
TCCGATTCGTCGATTCCAGATTCCGATCAGAGAAAATGGATTGCTAAATT
GAAGAAGAAATCGAAAAACGATGCGGACTTGGAATATATGATTTTCAATC
CGAAAAAAATGGGTTACCGAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0015078proton transmembrane transporter activity
Vocabulary: Biological Process
TermDefinition
GO:0015991ATP hydrolysis coupled proton transport
Vocabulary: INTERPRO
TermDefinition
IPR008389ATPase_V0-cplx_e1/e2_su