EX569439

EST Overview


Name	EX569439
Unique Name	EX569439
Type	EST
Organism	Pisum sativum (pea)
Sequence length	796

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX569439 vs. TrEMBL
Match: C6TKB8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 3.466e-76
Identity = 152/192 (79.17%), Postives = 165/192 (85.94%), Query Frame = 1

Query:   28 MDSSRSPSVGGNG------TPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLEH 585
            MDSS+S ++GGNG      T I Q ND AASA GA+DSMQ L Q++ SI+KTLGLIHQL  TVS F+AA QMPLLQRINGLVAELDNMVKLAE CNIQVPMEVVNLIDDGKNPDEFT+DVIN+CIAKNQITKGKTDA K+LRKHL EELEQNFPDEVETFRESR A+AAELKR  QAQS LPNGD+RVK EH
Sbjct:    1 MDSSQSAALGGNGGSGGNGTLISQINDMAASATGADDSMQKLNQVSNSIQKTLGLIHQLYLTVSTFNAAFQMPLLQRINGLVAELDNMVKLAEKCNIQVPMEVVNLIDDGKNPDEFTKDVINSCIAKNQITKGKTDALKSLRKHLLEELEQNFPDEVETFRESRTAAAAELKR--QAQSALPNGDVRVKSEH 190

[Close]

BLAST of EX569439 vs. TrEMBL
Match: B9SPF4_RICCO (Nut2, putative OS=Ricinus communis GN=RCOM_0085590 PE=4 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 1.459e-66
Identity = 134/187 (71.66%), Postives = 155/187 (82.89%), Query Frame = 1

Query:   28 MDSSRSPSVG--GNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            MDSS++ +VG  GNG    Q NDTAA+AA A+D  QNL  +  SI+KTLGL+HQL  TVS+F+ A Q+PLLQR+NGLV ELD M KL+E CNIQVP+EV+NLIDDGKNPDEFTRDVIN+CIAKNQ+TKGKTDAFK LRKHL EELEQ FPDEVE++RE RA SAAE KRL QAQS LPNGD++VK E
Sbjct:    1 MDSSQNATVGSGGNGMLPSQVNDTAAAAA-ADDPKQNLNNVINSIQKTLGLLHQLYLTVSSFNTASQLPLLQRLNGLVLELDKMAKLSEKCNIQVPLEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKGLRKHLLEELEQAFPDEVESYREIRAISAAESKRLAQAQSSLPNGDVKVKSE 186

[Close]

BLAST of EX569439 vs. TrEMBL
Match: D7TVR2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_25.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019654001 PE=4 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 1.906e-66
Identity = 134/197 (68.02%), Postives = 159/197 (80.71%), Query Frame = 1

Query:   16 RETQMDSSRSPSVG-----GNGTPIFQTNDTAASA---AGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            R  +M+SS++ + G     GNG  I Q N  AA+A   AG+ D  QNL Q+  SI+KTLGL+HQL  TVS+F+ A Q+PLLQR+N LV+ELDNM KL+E CNIQVPMEV+NLID+GKNPDEFTRDV+N+CIAKNQITKGKTD+FK LR+HL EELEQ FPDEVE++RE RAASAAE KRL QAQSVLPNGDL+VK E
Sbjct:   87 RTKEMESSQNAASGTGVSGGNGITIPQINAAAAAATAGAGSEDPKQNLNQVINSIQKTLGLLHQLYLTVSSFNVASQLPLLQRLNALVSELDNMTKLSEKCNIQVPMEVLNLIDEGKNPDEFTRDVLNSCIAKNQITKGKTDSFKGLRRHLLEELEQAFPDEVESYREIRAASAAESKRLAQAQSVLPNGDLKVKTE 283

[Close]

BLAST of EX569439 vs. TrEMBL
Match: B9GK75_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_815000 PE=4 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 1.906e-66
Identity = 129/177 (72.88%), Postives = 149/177 (84.18%), Query Frame = 1

Query:   52 VGGNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            +GGNG      NDTAA+ A  +D  QNL Q+  SI+KTLGL+HQL  TVS+F+ A Q+PLLQR+NGLV ELDNMVKL+E CNIQVPMEV+NLIDDGKNPDEF+RDVIN+CIAKNQ+TKGKTDAFK+LRKHL EELEQ FPDEVE++RE RA SAAE KRL QAQS LPNGD++VK E
Sbjct:   14 IGGNGMLAAPVNDTAAATA-VDDPKQNLNQVINSIQKTLGLLHQLYLTVSSFNTASQLPLLQRLNGLVTELDNMVKLSEKCNIQVPMEVLNLIDDGKNPDEFSRDVINSCIAKNQVTKGKTDAFKSLRKHLLEELEQTFPDEVESYREIRAMSAAEAKRLAQAQSSLPNGDVKVKAE 189

[Close]

BLAST of EX569439 vs. TrEMBL
Match: Q93WN1_ARATH (At1g26660/T24P13_4 OS=Arabidopsis thaliana GN=At1g26665 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 3.043e-64
Identity = 123/188 (65.43%), Postives = 154/188 (81.91%), Query Frame = 1

Query:   28 MDSSRSPSVG---GNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            MD +++ S G    NGT  +QTND  ++   A+DS +NL Q+  SIEKTLG++HQL  TV++F+ A Q+ LLQR+N LV ELDNM KL+E CNIQ+PMEV+NLIDDGKNPDEFT+DV+N+CIA+NQ+TKGKTDAFK+LRKH+ EELEQNFPDEV+ +RE RA+SAAE KRL Q+QSVLPNGD +VK E
Sbjct:    1 MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAEAKRLAQSQSVLPNGDAKVKNE 188

[Close]

BLAST of EX569439 vs. TrEMBL
Match: B9GY35_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_554563 PE=4 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 3.043e-64
Identity = 128/188 (68.09%), Postives = 151/188 (80.32%), Query Frame = 1

Query:   28 MDSSRSPSVGGNGTPIFQT--NDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLEH 585
            MDSS++  +G  G  +  T  NDTAA+    +D  QNL Q+  SI+KTLG +HQL  TVS+F+ A Q+PLLQR+NGLV ELDNMVKL+E CNIQVPMEV+NLIDDGKNPDEFTR VIN+CI KNQ+TKGKTD FK+LRKHL EELEQ FPDEVE++RE RA SAAE KRL Q+QS LPNGD++VK EH
Sbjct:    1 MDSSQNTVLGSGGNGMLATPLNDTAAAV---DDPKQNLNQVIDSIQKTLGQLHQLYLTVSSFNTASQLPLLQRLNGLVTELDNMVKLSERCNIQVPMEVLNLIDDGKNPDEFTRGVINSCITKNQVTKGKTDGFKSLRKHLLEELEQTFPDEVESYREIRAMSAAEAKRLAQSQSSLPNGDVKVKPEH 185

[Close]

BLAST of EX569439 vs. TrEMBL
Match: Q3ED58_ARATH (Putative uncharacterized protein At1g26665.2 OS=Arabidopsis thaliana GN=At1g26665 PE=4 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 1.972e-63
Identity = 122/188 (64.89%), Postives = 153/188 (81.38%), Query Frame = 1

Query:   28 MDSSRSPSVG---GNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            MD +++ S G    NGT  +QTND  ++   A+DS +NL Q+  SIEKTLG++HQL  TV++F+ A Q+ LLQR+N LV ELDNM KL+E CNIQ+PMEV+NLIDDGKNPDEFT+DV+N+CIA+NQ+TKGKTDAFK+LRKH+ EELEQNFPDEV+ +RE RA+SAA  KRL Q+QSVLPNGD +VK E
Sbjct:    1 MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAVTKRLAQSQSVLPNGDAKVKNE 188

[Close]

BLAST of EX569439 vs. TrEMBL
Match: C5X656_SORBI (Putative uncharacterized protein Sb02g030650 OS=Sorghum bicolor GN=Sb02g030650 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 6.345e-62
Identity = 122/171 (71.35%), Postives = 140/171 (81.87%), Query Frame = 1

Query:   85 NDTAASAAGA----NDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLEH 585
            N   AS AG     + S QNL Q+  SI+KTLGL+HQL  TVS+F++A Q+PLLQR+N LVAELD M KLAE CNIQVPMEVVNLIDDGKNPDEFTRDV+N+CIAKNQITKGKTDAFK+LRKHL EELEQ FP++VE +RE RA SAAE KRL Q+Q  LPNGD++VK EH
Sbjct:   18 NGVQASGAGGERPEDASKQNLAQVTSSIQKTLGLLHQLNLTVSSFNSASQLPLLQRLNALVAELDTMQKLAEGCNIQVPMEVVNLIDDGKNPDEFTRDVLNSCIAKNQITKGKTDAFKSLRKHLLEELEQAFPEDVEAYREIRATSAAEAKRLAQSQGTLPNGDVKVKAEH 188

[Close]

BLAST of EX569439 vs. TrEMBL
Match: B6T6M5_MAIZE (Mediator complex subunit 10 CG5057-PA OS=Zea mays PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 7.016e-61
Identity = 120/167 (71.86%), Postives = 137/167 (82.04%), Query Frame = 1

Query:   97 ASAAGA----NDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLEH 585
            AS AG     + S QNL Q+  SI+KTLGL+HQL  TVS+F++A Q+PLLQR+N LVAELD M KLAE CNIQVPMEVVNLIDDGKNPDEFTRDV+N+CI KNQITKGKTDAFK LRKHL EELEQ FP++VE +RE RA SAAE KRL Q+Q  LPNGD++VK EH
Sbjct:   24 ASGAGGERPEDASKQNLAQVTSSIQKTLGLLHQLNLTVSSFNSASQLPLLQRLNALVAELDTMQKLAEGCNIQVPMEVVNLIDDGKNPDEFTRDVLNSCIVKNQITKGKTDAFKGLRKHLLEELEQAFPEDVEAYREIRATSAAEAKRLAQSQGTLPNGDVKVKAEH 190

[Close]

BLAST of EX569439 vs. TrEMBL
Match: B6TNI2_MAIZE (Mediator complex subunit 10 CG5057-PA OS=Zea mays PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 3.850e-59
Identity = 119/171 (69.59%), Postives = 137/171 (80.12%), Query Frame = 1

Query:   85 NDTAASAAGA----NDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLEH 585
            N   AS AG     + S QNL Q+  SI+KTLGL+HQL  TVS+F++A Q+PLLQR+N LVA LD M KLAE CNIQVPMEVVNLIDDGKNPDEFTRDV+N+CIAKNQITKGKTDAFK+LRKHL EELEQ FP++VE +RE RA SAAE KRL Q+   L NGD++VK EH
Sbjct:   17 NGVQASGAGGERPEDASKQNLAQVTSSIQKTLGLLHQLNLTVSSFNSASQLPLLQRLNALVAXLDTMQKLAEGCNIQVPMEVVNLIDDGKNPDEFTRDVLNSCIAKNQITKGKTDAFKSLRKHLLEELEQAFPEDVEAYREIRATSAAEAKRLAQSHGNLLNGDVKVKAEH 187

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_AEDAE (Mediator of RNA polymerase II transcription subunit 10 OS=Aedes aegypti GN=MED10 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 8.384e-16
Identity = 40/123 (32.52%), Postives = 70/123 (56.91%), Query Frame = 1

Query:  133 LKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            L+ +   +E  +  + Q+   VS+F    Q  L Q+I  LV  L  + KL    ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L +EL++ FP E+  ++  R
Sbjct:    5 LENLENHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQVDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELDETFPTEIAKYKAIR 127

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_ANOGA (Mediator of RNA polymerase II transcription subunit 10 OS=Anopheles gambiae GN=MED10 PE=3 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 1.868e-15
Identity = 40/123 (32.52%), Postives = 69/123 (56.10%), Query Frame = 1

Query:  133 LKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            L+ +   +E  +  + Q+   VS+F    Q  L Q+I  LV  L  + KL    ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L +EL + FP E+  ++  R
Sbjct:    5 LENLENHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQIDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELSETFPVEISKYKAIR 127

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_MOUSE (Mediator of RNA polymerase II transcription subunit 10 OS=Mus musculus GN=Med10 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 7.847e-14
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N +V  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  +R  R
Sbjct:    3 EKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLSQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_PONAB (Mediator of RNA polymerase II transcription subunit 10 OS=Pongo abelii GN=MED10 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.025e-13
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N +V  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  +R  R
Sbjct:    3 EKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_HUMAN (Mediator of RNA polymerase II transcription subunit 10 OS=Homo sapiens GN=MED10 PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.025e-13
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N +V  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  +R  R
Sbjct:    3 EKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_BOVIN (Mediator of RNA polymerase II transcription subunit 10 OS=Bos taurus GN=MED10 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.025e-13
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N +V  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  +R  R
Sbjct:    3 EKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_XENTR (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus tropicalis GN=med10 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.339e-13
Identity = 41/126 (32.54%), Postives = 71/126 (56.35%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N LV  L ++ K  +  + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  ++  R
Sbjct:    3 EKFDNLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVTGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_XENLA (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus laevis GN=med10 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.982e-13
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    +   +EK +  I QL   VS+F  + Q  L Q++N LV  L ++ K  +  + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L +EL + FP+++  ++  R
Sbjct:    3 EKFDNLEDHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVRGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAVR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_MACFA (Mediator of RNA polymerase II transcription subunit 10 OS=Macaca fascicularis GN=MED10 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.643e-13
Identity = 40/126 (31.75%), Postives = 69/126 (54.76%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESR 501
            +    + + +EK +  I QL   VS+F  + Q  L Q++N +V  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L + L + FP+++  +R  R
Sbjct:    3 EKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQGLSKVFPEDMAKYRSIR 128

[Close]

BLAST of EX569439 vs. SwissProt
Match: MED10_DANRE (Mediator of RNA polymerase II transcription subunit 10 OS=Danio rerio GN=med10 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.524e-12
Identity = 38/123 (30.89%), Postives = 67/123 (54.47%), Query Frame = 1

Query:  127 QNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCN-IQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFR 492
            +    + + +EK +  I QL   VS+F  + Q  L Q++N ++  L ++ K  +  + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D     +  L  EL + FP+E+  ++
Sbjct:    3 EKFDNLEEHLEKFVENIRQLGIIVSDFQPSSQTGLNQKLNFMITGLQDIEKCRQQLHDINVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLTKFKSLLISELGKVFPEEMAKYK 125

[Close]

BLAST of EX569439 vs. TAIR peptide
Match: AT1G26665.1 (| Symbols: | Mediator complex, subunit Med10 | chr1:9214510-9215426 FORWARD LENGTH=189)

HSP 1 Score: 249.21 bits (635), Expect = 1.935e-66
Identity = 123/188 (65.43%), Postives = 154/188 (81.91%), Query Frame = 1

Query:   28 MDSSRSPSVG---GNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            MD +++ S G    NGT  +QTND  ++   A+DS +NL Q+  SIEKTLG++HQL  TV++F+ A Q+ LLQR+N LV ELDNM KL+E CNIQ+PMEV+NLIDDGKNPDEFT+DV+N+CIA+NQ+TKGKTDAFK+LRKH+ EELEQNFPDEV+ +RE RA+SAAE KRL Q+QSVLPNGD +VK E
Sbjct:    1 MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAEAKRLAQSQSVLPNGDAKVKNE 188

[Close]

BLAST of EX569439 vs. TAIR peptide
Match: AT1G26665.2 (| Symbols: | Mediator complex, subunit Med10 | chr1:9214510-9215426 FORWARD LENGTH=189)

HSP 1 Score: 246.514 bits (628), Expect = 1.255e-65
Identity = 122/188 (64.89%), Postives = 153/188 (81.38%), Query Frame = 1

Query:   28 MDSSRSPSVG---GNGTPIFQTNDTAASAAGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVINNCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            MD +++ S G    NGT  +QTND  ++   A+DS +NL Q+  SIEKTLG++HQL  TV++F+ A Q+ LLQR+N LV ELDNM KL+E CNIQ+PMEV+NLIDDGKNPDEFT+DV+N+CIA+NQ+TKGKTDAFK+LRKH+ EELEQNFPDEV+ +RE RA+SAA  KRL Q+QSVLPNGD +VK E
Sbjct:    1 MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAVTKRLAQSQSVLPNGDAKVKNE 188

[Close]

BLAST of EX569439 vs. TAIR peptide
Match: AT5G41910.1 (| Symbols: MED10A | Mediator complex, subunit Med10 | chr5:16778160-16779032 REVERSE LENGTH=186)

HSP 1 Score: 212.231 bits (539), Expect = 2.622e-55
Identity = 108/160 (67.50%), Postives = 129/160 (80.62%), Query Frame = 1

Query:  106 AGANDSMQNLKQINKSIEKTLGLIHQLTPTVSNFSAALQMPLLQRINGLVAELDNMVKLAENCNIQVPMEVVNLIDDGKNPDEFTRDVIN-NCIAKNQITKGKTDAFKNLRKHLFEELEQNFPDEVETFRESRAASAAELKRLVQAQSVLPNGDLRVKLE 582
            A  +DS + L QI  S +K L L+HQL  TVS+F+ A Q+ LLQR+N LV ELDNM KL++ CNIQVP+EV+NLIDDGKNPDEFT+DV+N NCIAKNQ+TKGK+DAFK LRKHL EELEQ FPDEV+ +R+ RA+ AAE KRL Q QSVLPNGD +VK E
Sbjct:   26 ATVDDSNEKLNQIINSNQKILELLHQLKLTVSSFTPASQLHLLQRLNSLVMELDNMAKLSDKCNIQVPIEVLNLIDDGKNPDEFTKDVLNKNCIAKNQVTKGKSDAFKGLRKHLLEELEQAFPDEVDRYRDIRASYAAEAKRLAQTQSVLPNGDAKVKSE 185

The following BLAST results are available for this feature:

BLAST of EX569439 vs. TrEMBL
BLAST of EX569439 vs. SwissProt
BLAST of EX569439 vs. TAIR peptide

BLAST of EX569439 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TKB8_SOYBN	3.466e-76	79.17	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SPF4_RICCO	1.459e-66	71.66	Nut2, putative OS=Ricinus communis GN=RCOM_0085590...	[more]
D7TVR2_VITVI	1.906e-66	68.02	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GK75_POPTR	1.906e-66	72.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q93WN1_ARATH	3.043e-64	65.43	At1g26660/T24P13_4 OS=Arabidopsis thaliana GN=At1g...	[more]
B9GY35_POPTR	3.043e-64	68.09	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q3ED58_ARATH	1.972e-63	64.89	Putative uncharacterized protein At1g26665.2 OS=Ar...	[more]
C5X656_SORBI	6.345e-62	71.35	Putative uncharacterized protein Sb02g030650 OS=So...	[more]
B6T6M5_MAIZE	7.016e-61	71.86	Mediator complex subunit 10 CG5057-PA OS=Zea mays ...	[more]
B6TNI2_MAIZE	3.850e-59	69.59	Mediator complex subunit 10 CG5057-PA OS=Zea mays ...	[more]

BLAST of EX569439 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
MED10_AEDAE	8.384e-16	32.52	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_ANOGA	1.868e-15	32.52	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_MOUSE	7.847e-14	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_PONAB	1.025e-13	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_HUMAN	1.025e-13	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_BOVIN	1.025e-13	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_XENTR	1.339e-13	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_XENLA	2.982e-13	32.54	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_MACFA	6.643e-13	31.75	Mediator of RNA polymerase II transcription subuni...	[more]
MED10_DANRE	2.524e-12	30.89	Mediator of RNA polymerase II transcription subuni...	[more]

BLAST of EX569439 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G26665.1	1.935e-66	65.43	\| Symbols: \| Mediator complex, subunit Med10 \| ch...	[more]
AT1G26665.2	1.255e-65	64.89	\| Symbols: \| Mediator complex, subunit Med10 \| ch...	[more]
AT5G41910.1	2.622e-55	67.50	\| Symbols: MED10A \| Mediator complex, subunit Med1...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019145	Mediator complex, subunit Med10	PFAM	PF09748	Med10	coord: 49..164 score: 2.4
None	No IPR available	PANTHER	PTHR13345	NUT2 AND UXT	coord: 94..185 score: 2.8
None	No IPR available	PANTHER	PTHR13345:SF2	NUT2	coord: 94..185 score: 2.8
None	No IPR available	SEG	seg	seg	coord: 13..27 score
None	No IPR available	SEG	seg	seg	coord: 68..79 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX569439 ID=EX569439; Name=EX569439; organism=Pisum sativum; type=EST; length=796bp
CGAGAAACTCCATTCAGAGAAACACAAATGGATTCATCACGAAGTCCATC
TGTTGGGGGAAATGGGACACCAATTTTTCAAACTAACGATACTGCAGCTT
CTGCAGCAGGAGCTAATGATTCTATGCAAAATCTGAAGCAGATCAACAAG
TCCATTGAGAAAACCTTGGGCCTTATCCATCAGCTTACCCCTACTGTCTC
TAACTTCAGTGCTGCTTTGCAAATGCCACTCCTCCAACGCATCAATGGAC
TTGTTGCAGAGCTTGACAACATGGTCAAATTGGCAGAAAACTGCAACATT
CAGGTTCCTATGGAGGTTGTAAATTTAATTGATGATGGGAAGAATCCAGA
TGAGTTTACCAGAGATGTTATAAACAACTGTATTGCAAAGAATCAGATCA
CCAAAGGAAAAACGGATGCTTTTAAGAATTTGCGCAAGCATCTATTTGAG
GAATTGGAGCAAAACTTCCCTGATGAGGTTGAGACATTTAGAGAGAGTCG
TGCTGCGTCTGCTGCTGAGTTGAAGCGTTTGGTGCAGGCACAAAGTGTAT
TGCCGAATGGAGATTTGAGAGTTAAACTAGAGCATTGAGTGATATATTTC
AGATGCATTTATATTACTCCTTTTCCCCTCTTTTTTCTTGACTTTATTTT
TAGGTGAAAACTATGGTACCTTCTAGTTTTATTTGGATTTTAATTCTCTG
CAGTGGATGGGTCCCAGTAAGATGCATTTTATGTAAATCAACTTCGTAAT
AATTGCAGGATCCAAAATATCACAAGAATGCGCTGAGTTCCTAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006357	regulation of transcription by RNA polymerase II

Vocabulary: **Molecular Function**
Term	Definition
GO:0016455	RNA polymerase II transcription mediator activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

Vocabulary: **INTERPRO**
Term	Definition
IPR019145	Mediator_Med10

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