EX570312

EST Overview
NameEX570312
Unique NameEX570312
TypeEST
OrganismPisum sativum (pea)
Sequence length298
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of EX570312 vs. TrEMBL
Match: O65335_PEA (SAR DNA-binding protein-2 OS=Pisum sativum GN=SARBP-2 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.522e-17
Identity = 44/45 (97.78%), Postives = 44/45 (97.78%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVTG 135
            DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDED PETPVTG
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDTPETPVTG 462
[Close]
BLAST of EX570312 vs. TrEMBL
Match: B7FLW5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.509e-14
Identity = 38/44 (86.36%), Postives = 40/44 (90.91%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVT 132
            DKD+KKG+GGLITPAK YNTAADSVIEP SNSAMDED PE PVT
Sbjct:  418 DKDRKKGAGGLITPAKAYNTAADSVIEPMSNSAMDEDTPEPPVT 461
[Close]
BLAST of EX570312 vs. TrEMBL
Match: O65334_PEA (SAR DNA-binding protein-1 OS=Pisum sativum GN=SARBP-1 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.474e-12
Identity = 36/40 (90.00%), Postives = 37/40 (92.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKDKKKG+GGLITPAKTYNTAADSVI  KSNSAMDED PE
Sbjct:  418 DKDKKKGAGGLITPAKTYNTAADSVIHQKSNSAMDEDTPE 457
[Close]
BLAST of EX570312 vs. TrEMBL
Match: Q70I27_LOTJA (SAR DNA-binding protein-like protein OS=Lotus japonicus PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.399e-9
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKD+KKG+GGLITPAKTYN +AD+VI  KS+SAMDED  E
Sbjct:  404 DKDRKKGAGGLITPAKTYNPSADAVISQKSDSAMDEDTHE 443
[Close]
BLAST of EX570312 vs. TrEMBL
Match: B9I857_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1096439 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.255e-7
Identity = 28/50 (56.00%), Postives = 35/50 (70.00%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAM-----DEDAPETPVTG 135
            DKD+KKG+GGLITPAKTYN +AD+++    NS       +E A E PVTG
Sbjct:  418 DKDRKKGAGGLITPAKTYNPSADAILGQTPNSTARKVEEEEPAKEAPVTG 467
[Close]
BLAST of EX570312 vs. SwissProt
Match: NOP5A_ARATH (Probable nucleolar protein 5-1 OS=Arabidopsis thaliana GN=NOP5-1 PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 2.300e-7
Identity = 24/27 (88.89%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIE 81
            DKDKKKGSGGLITPAKTYNTAADS+++
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSLLQ 444
[Close]
BLAST of EX570312 vs. TAIR peptide
Match: AT5G27120.1 (| Symbols: | NOP56-like pre RNA processing ribonucleoprotein | chr5:9541287-9543684 FORWARD LENGTH=533)

HSP 1 Score: 54.299 bits (129), Expect = 1.811e-8
Identity = 24/27 (88.89%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIE 81
            DKDKKKGSGGLITPAKTYNTAADS+++
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSLLQ 444
[Close]
BLAST of EX570312 vs. TAIR peptide
Match: AT3G05060.1 (| Symbols: | NOP56-like pre RNA processing ribonucleoprotein | chr3:1413174-1415564 REVERSE LENGTH=533)

HSP 1 Score: 49.6766 bits (117), Expect = 4.461e-7
Identity = 26/54 (48.15%), Postives = 33/54 (61.11%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVI--------------EPKSNSAMDEDAPE 120
            +KDKK GSGGLITPAKTYNTAADS++              + K    ++E+ PE
Sbjct:  419 NKDKKMGSGGLITPAKTYNTAADSLLGETSAKSEEPSKKKDKKKKKKVEEEKPE 472
[Close]
BLAST of EX570312 vs. TAIR peptide
Match: AT5G27120.1 (| Symbols: | NOP56-like pre RNA processing ribonucleoprotein | chr5:9541287-9543684 FORWARD LENGTH=533)

HSP 1 Score: 54.299 bits (129), Expect = 1.811e-8
Identity = 24/27 (88.89%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIE 81
            DKDKKKGSGGLITPAKTYNTAADS+++
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSLLQ 444
[Close]
BLAST of EX570312 vs. TAIR peptide
Match: AT3G05060.1 (| Symbols: | NOP56-like pre RNA processing ribonucleoprotein | chr3:1413174-1415564 REVERSE LENGTH=533)

HSP 1 Score: 49.6766 bits (117), Expect = 4.461e-7
Identity = 26/54 (48.15%), Postives = 33/54 (61.11%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVI--------------EPKSNSAMDEDAPE 120
            +KDKK GSGGLITPAKTYNTAADS++              + K    ++E+ PE
Sbjct:  419 NKDKKMGSGGLITPAKTYNTAADSLLGETSAKSEEPSKKKDKKKKKKVEEEKPE 472
[Close]
BLAST of EX570312 vs. TrEMBL
Match: O65335_PEA (SAR DNA-binding protein-2 OS=Pisum sativum GN=SARBP-2 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.903e-17
Identity = 44/45 (97.78%), Postives = 44/45 (97.78%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVTG 135
            DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDED PETPVTG
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDTPETPVTG 462
[Close]
BLAST of EX570312 vs. TrEMBL
Match: B7FLW5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.388e-14
Identity = 38/44 (86.36%), Postives = 40/44 (90.91%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVT 132
            DKD+KKG+GGLITPAK YNTAADSVIEP SNSAMDED PE PVT
Sbjct:  418 DKDRKKGAGGLITPAKAYNTAADSVIEPMSNSAMDEDTPEPPVT 461
[Close]
BLAST of EX570312 vs. TrEMBL
Match: O65334_PEA (SAR DNA-binding protein-1 OS=Pisum sativum GN=SARBP-1 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.844e-12
Identity = 36/40 (90.00%), Postives = 37/40 (92.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKDKKKG+GGLITPAKTYNTAADSVI  KSNSAMDED PE
Sbjct:  418 DKDKKKGAGGLITPAKTYNTAADSVIHQKSNSAMDEDTPE 457
[Close]
BLAST of EX570312 vs. TrEMBL
Match: Q70I27_LOTJA (SAR DNA-binding protein-like protein OS=Lotus japonicus PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.250e-9
Identity = 30/40 (75.00%), Postives = 35/40 (87.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKD+KKG+GGLITPAKTYN +AD+VI  KS+SAMDED  E
Sbjct:  404 DKDRKKGAGGLITPAKTYNPSADAVISQKSDSAMDEDTHE 443
[Close]
BLAST of EX570312 vs. Soybean peptides
Match: Glyma12g04400.1|PACid:16286369 ()

HSP 1 Score: 65.0846 bits (157), Expect = 1.607e-11
Identity = 32/43 (74.42%), Postives = 35/43 (81.40%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPV 129
            DKD+KKG+GGLITPAKTYN +ADSVI    NSAMDEDA E  V
Sbjct:  418 DKDRKKGAGGLITPAKTYNPSADSVIGQMVNSAMDEDAQEPSV 460
[Close]
BLAST of EX570312 vs. Soybean peptides
Match: Glyma11g12200.1|PACid:16283605 ()

HSP 1 Score: 61.6178 bits (148), Expect = 1.777e-10
Identity = 30/43 (69.77%), Postives = 35/43 (81.40%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPV 129
            DKD+KKG+GGLITPAKTYN +ADSVI    +SA+DEDA E  V
Sbjct:  418 DKDRKKGAGGLITPAKTYNPSADSVIGQMVDSAIDEDAQEPSV 460
[Close]
BLAST of EX570312 vs. SwissProt
Match: NOP5A_ARATH (Probable nucleolar protein 5-1 OS=Arabidopsis thaliana GN=NOP5-1 PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 2.340e-7
Identity = 24/27 (88.89%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIE 81
            DKDKKKGSGGLITPAKTYNTAADS+++
Sbjct:  418 DKDKKKGSGGLITPAKTYNTAADSLLQ 444
[Close]
BLAST of EX570312 vs. Medicago proteins
Match: IMGA|Medtr4g070910.2 (SAR DNA-binding protein-1 (AHRD V1 *-*- O65334_PEA) chr04_pseudomolecule_IMGAG_V3.5 22290751-22295559 F EGN_Mt100125 20100825)

HSP 1 Score: 83.1889 bits (204), Expect = 3.502e-17
Identity = 39/44 (88.64%), Postives = 41/44 (93.18%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVT 132
            DKD+KKG+GGLITPAKTYNTAADSVIEP SNSAMDED PE PVT
Sbjct:   33 DKDRKKGAGGLITPAKTYNTAADSVIEPMSNSAMDEDTPEPPVT 76
[Close]
BLAST of EX570312 vs. Medicago proteins
Match: IMGA|Medtr4g070910.1 (SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA); contains Interpro domain(s) IPR002687 Pre-mRNA processing ribonucleoprotein, binding region chr04_pseudomolecule_IMGAG_V3.5 22290751-22295559 F EGN_Mt100125 20100825)

HSP 1 Score: 83.1889 bits (204), Expect = 3.502e-17
Identity = 39/44 (88.64%), Postives = 41/44 (93.18%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPETPVT 132
            DKD+KKG+GGLITPAKTYNTAADSVIEP SNSAMDED PE PVT
Sbjct:  418 DKDRKKGAGGLITPAKTYNTAADSVIEPMSNSAMDEDTPEPPVT 461
[Close]
BLAST of EX570312 vs. Medicago proteins
Match: IMGA|Medtr5g010260.2 (SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA); contains Interpro domain(s) IPR002687 Pre-mRNA processing ribonucleoprotein, binding region chr05_pseudomolecule_IMGAG_V3.5 2467131-2463464 F EGN_Mt100125 20100825)

HSP 1 Score: 70.0922 bits (170), Expect = 3.068e-13
Identity = 32/40 (80.00%), Postives = 37/40 (92.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKD+KKG+GGLITPAKTYNTAADS+I+ KS+SAMDED  E
Sbjct:  308 DKDRKKGAGGLITPAKTYNTAADSIIDKKSDSAMDEDTEE 347
[Close]
BLAST of EX570312 vs. Medicago proteins
Match: IMGA|Medtr5g010260.1 (SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA); contains Interpro domain(s) IPR002687 Pre-mRNA processing ribonucleoprotein, binding region chr05_pseudomolecule_IMGAG_V3.5 2467131-2463464 F EGN_Mt100125 20100825)

HSP 1 Score: 70.0922 bits (170), Expect = 3.068e-13
Identity = 32/40 (80.00%), Postives = 37/40 (92.50%), Query Frame = 1 
Query:    1 DKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDAPE 120
            DKD+KKG+GGLITPAKTYNTAADS+I+ KS+SAMDED  E
Sbjct:  418 DKDRKKGAGGLITPAKTYNTAADSIIDKKSDSAMDEDTEE 457
The following BLAST results are available for this feature:
BLAST of EX570312 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
O65335_PEA1.522e-1797.78SAR DNA-binding protein-2 OS=Pisum sativum GN=SARB... [more]
B7FLW5_MEDTR3.509e-1486.36Putative uncharacterized protein OS=Medicago trunc... [more]
O65334_PEA1.474e-1290.00SAR DNA-binding protein-1 OS=Pisum sativum GN=SARB... [more]
Q70I27_LOTJA3.399e-975.00SAR DNA-binding protein-like protein OS=Lotus japo... [more]
B9I857_POPTR9.255e-756.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of EX570312 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
NOP5A_ARATH2.300e-788.89Probable nucleolar protein 5-1 OS=Arabidopsis thal... [more]
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BLAST of EX570312 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G27120.11.811e-888.89| Symbols: | NOP56-like pre RNA processing ribonu... [more]
AT3G05060.14.461e-748.15| Symbols: | NOP56-like pre RNA processing ribonu... [more]
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BLAST of EX570312 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G27120.11.811e-888.89| Symbols: | NOP56-like pre RNA processing ribonu... [more]
AT3G05060.14.461e-748.15| Symbols: | NOP56-like pre RNA processing ribonu... [more]
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BLAST of EX570312 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 4
Match NameE-valueIdentityDescription
O65335_PEA1.903e-1797.78SAR DNA-binding protein-2 OS=Pisum sativum GN=SARB... [more]
B7FLW5_MEDTR4.388e-1486.36Putative uncharacterized protein OS=Medicago trunc... [more]
O65334_PEA1.844e-1290.00SAR DNA-binding protein-1 OS=Pisum sativum GN=SARB... [more]
Q70I27_LOTJA4.250e-975.00SAR DNA-binding protein-like protein OS=Lotus japo... [more]
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BLAST of EX570312 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2
Match NameE-valueIdentityDescription
Glyma12g04400.1|PACid:162863691.607e-1174.42[more]
Glyma11g12200.1|PACid:162836051.777e-1069.77[more]
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BLAST of EX570312 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
NOP5A_ARATH2.340e-788.89Probable nucleolar protein 5-1 OS=Arabidopsis thal... [more]
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BLAST of EX570312 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 4
Match NameE-valueIdentityDescription
IMGA|Medtr4g070910.23.502e-1788.64SAR DNA-binding protein-1 (AHRD V1 *-*- O65334_PEA... [more]
IMGA|Medtr4g070910.13.502e-1788.64SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA... [more]
IMGA|Medtr5g010260.23.068e-1380.00SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA... [more]
IMGA|Medtr5g010260.13.068e-1380.00SAR DNA-binding protein-1 (AHRD V1 **** O65334_PEA... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 46..54
score
NoneNo IPR availableSEGsegsegcoord: 28..42
score
NoneNo IPR availableSEGsegsegcoord: 2..10
score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 46..54
score
NoneNo IPR availableSEGsegsegcoord: 28..42
score
NoneNo IPR availableSEGsegsegcoord: 2..10
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX570312 ID=EX570312; Name=EX570312; organism=Pisum sativum; type=EST; length=298bp
GACAAGGATAAAAAGAAAGGATCTGGAGGACTGATAACGCCTGCCAAGAC
ATATAATACTGCAGCTGATTCAGTTATTGAGCCCAAATCTAATTCTGCAA
TGGACGAAGATGCACCAGAGACTCCTGTTACGGGCAAAAAGAAAGAAAAA
AAGAAGAAAAAAGACGTTTAGAGCGGAATGTATGTATAGTCACCAGCTAA
TCAAAATGGTGACTGGTAACCATTTTTGTATACTTTGGGAGCTCAGTAGT
TGTTTTGGTGTCATATTTGCTTTTCATGTTAATGCAATTTGTAGGTCT
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