GH720502

EST Overview


Name	GH720502
Unique Name	GH720502
Type	EST
Organism	Pisum sativum (pea)
Sequence length	520

Libraries


Library Name	Type
LIBEST_024271 552NI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9GLC6_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP716D2v1|CYP716D3v1 PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 4.998e-49
Identity = 93/154 (60.39%), Postives = 117/154 (75.97%), Query Frame = 1

Query:   25 KAADVXXXXXXXXXXXXXVDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            KAAD                L++K ASPTQDLLSHLLVTSD SG+F+SE EI+DN+LLLLFA HDT+ S I+ +MKYL  LPEVY+ VL+EQ++I++ KE GELL+WED+QKM+YSWNV SE++RL PP+ GA+R A+ D TYA YTIP  WK+
Sbjct:  161 KAADAIKEELRLISRRRRAALDKKMASPTQDLLSHLLVTSDASGKFLSETEIVDNILLLLFASHDTTTSVITCVMKYLAELPEVYQTVLREQIDIAKSKEPGELLKWEDIQKMRYSWNVVSEIMRLMPPVRGAFREAIVDFTYAGYTIPKGWKV 314

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B8Y882_STERE (Cytochrome P450 monooxygenase (Fragment) OS=Stevia rebaudiana PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 6.110e-47
Identity = 89/139 (64.03%), Postives = 115/139 (82.73%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWN 495
            ++L+E K S +QDLLSHLL + D +G F++E EI+DN+LLLLFAGHDTS  +I+L+MK LG   +VY++VLKEQLEIS+GKE GELL+WED+QKMKYSW+V  EV+RL+PP+ GAYR A+ D+ YA YTIP  WKLHW+
Sbjct:  243 LELKEGKPSSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVYDKVLKEQLEISKGKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAGYTIPKGWKLHWS 381

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9GLC4_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP716D1 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 8.823e-46
Identity = 85/141 (60.28%), Postives = 113/141 (80.14%), Query Frame = 1

Query:   85 LEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            L++K  S  +DLLSHLLVT+D SG+ +SE EI+DNML+LLF  H+T+ SA++ ++KYL  +PEVYE VL+EQL+I++ KE GELL+WED+QKMKYSW V SEVLR+ PPI+G +R A+ D TYA YTIP  WKL+W+  T+
Sbjct:  250 LDKKMESHRKDLLSHLLVTADESGKLLSESEIVDNMLMLLFVSHETTTSAMTCVIKYLAEMPEVYEMVLREQLDIAKSKEAGELLKWEDIQKMKYSWRVVSEVLRMIPPISGTFRQAIVDFTYAGYTIPKGWKLYWSPNTT 390

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9GLC5_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP716D2v1|CYP716D3v1 PE=3 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 5.719e-45
Identity = 89/157 (56.69%), Postives = 114/157 (72.61%), Query Frame = 1

Query:   25 KAADVXXXXXXXXXXXXXVDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWN 495
            KAAD                L++K  S  +DLLSHLLVT+D SG+ +SE EI+DNML+LLFA HDT+ SA++ +MKYL  LPEVYE VL+EQL+I++ KE GELL+WED+Q+M+YS NV SEVLR+ PPI G +R A+ D TYA Y IP  WKL+W+
Sbjct:  161 KAADAIKEELRLIARRRREALDKKMESHRKDLLSHLLVTTDASGKLLSESEIVDNMLMLLFASHDTTTSAMTCVMKYLAELPEVYEMVLREQLDIAKSKEAGELLKWEDIQRMRYSCNVVSEVLRMIPPIRGTFRKALVDFTYAGYKIPKGWKLYWS 317

[Close]

BLAST of GH720502 vs. TrEMBL
Match: Q0NZP1_STERE (Ent-kaurenoic acid 13-hydroxylase OS=Stevia rebaudiana GN=KA13H PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.173e-44
Identity = 86/139 (61.87%), Postives = 112/139 (80.58%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWN 495
            ++L+E KAS +QDLLSHLL + D +G F++E EI+DN+LLLLFAGHDTS  +I+L+MK LG   +VY++VLKEQLEIS+ KE  E L+WED+QKMKYSW+V  EV+RL+PP+ G YR A+ D+ YA YTIP  WKLHW+
Sbjct:  243 LELKEGKASSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVYDKVLKEQLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAGYTIPKGWKLHWS 381

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9RHG4_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1525810 PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 6.323e-44
Identity = 79/142 (55.63%), Postives = 110/142 (77.46%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            DL E KA+P QD+LSH+LV +D  G+ + EV I D ++ LL  GHDT+ + I+ ++K+L  LP++Y+QVLKEQLEI++ KE GELL WED+QKMKYSWNVA EV+RL+PP+ G++R A+ D  YA ++IP  WKL+W+T T+
Sbjct:  244 DLAENKATPMQDILSHMLVATDEEGQRLGEVGIADKIISLLIGGHDTASATITFVVKFLAELPDIYDQVLKEQLEIAKSKEPGELLTWEDIQKMKYSWNVACEVMRLAPPLQGSFREALHDFDYAGFSIPKGWKLYWSTHTT 385

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9SPT3_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0205440 PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.409e-43
Identity = 80/143 (55.94%), Postives = 111/143 (77.62%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            +DL E KASPTQD+LSH+L T+D    +M+E++I D +L LL  GHDT+ +AI+ ++KYL  +P+VY +VL+EQ+EI++ K  GELL WED+QKM+YSWNVA EV+RL+PP+ GA+R AM D TYA +TIP  WKL+W   ++
Sbjct:  241 IDLAENKASPTQDILSHMLTTAD---EYMNEMDIADKILGLLIGGHDTASAAITFVVKYLAEMPQVYNKVLEEQMEIAKAKAAGELLNWEDIQKMRYSWNVACEVMRLAPPLQGAFREAMTDFTYAGFTIPKGWKLYWGANST 380

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9HBL7_POPTR (Cytochrome P450 OS=Populus trichocarpa GN=CYP716A8 PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.409e-43
Identity = 82/162 (50.62%), Postives = 116/162 (71.60%), Query Frame = 1

Query:   22 VKAADVXXXXXXXXXXXXXVDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            VKA++V             +DL E KASPTQD+LSH+L T+D +G+ M +++I D +L LL  GHDT+ +AI+ I+KYL  LP VY ++L+EQ EI++ K  GELL WED+Q+M+YSWNVA EV+R++PP+ GA+R AM +  YA +TIP  WKL+W+  T+
Sbjct:  231 VKASNVVHKELLKIIKQRKIDLAENKASPTQDILSHMLTTADDNGQCMKKIDIADKILGLLVGGHDTASAAITFIVKYLAELPHVYNKLLEEQREIAKTKTPGELLNWEDIQRMRYSWNVACEVMRVAPPLQGAFREAMTEFNYAGFTIPKGWKLYWSANTT 392

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9S060_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1299050 PE=4 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.840e-43
Identity = 86/120 (71.67%), Postives = 105/120 (87.50%), Query Frame = 1

Query:   85 LEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKD 444
            L++K  SPTQDLLS LLVT+DTSGRF+SE EI+DN+L LLFAGHDTSRSA +L+MK LG LPEVY +VLKEQ EI++ KE G++LQWE +QKMKYSWNVASE++RLSPP+NGA+R A+ D
Sbjct:  112 LDKKLVSPTQDLLSLLLVTADTSGRFLSETEIVDNILALLFAGHDTSRSARTLLMKCLGELPEVYAKVLKEQTEIAKCKEPGQMLQWEHIQKMKYSWNVASEIMRLSPPVNGAFREALVD 231

[Close]

BLAST of GH720502 vs. TrEMBL
Match: B9SAB8_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1237360 PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 9.130e-43
Identity = 78/143 (54.55%), Postives = 110/143 (76.92%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            +DL E KAS TQD+LSH+L+TSD  G+FM+E++I D +L LL  GHDT+ +A + I+KYL  LP++Y+ V KEQ+EI++ K EGELL WED+QKMKYSWNVA EV+R++PP+ GA+R A+ D  +  + IP  WKL+W+  ++
Sbjct:  247 IDLAEGKASGTQDILSHMLLTSDEDGKFMNEMDIADKILGLLIGGHDTASAACTFIIKYLAELPQIYDAVYKEQMEIAKSKGEGELLNWEDIQKMKYSWNVACEVMRVAPPLQGAFREAINDFIFNGFYIPKGWKLYWSANST 389

[Close]

BLAST of GH720502 vs. SwissProt
Match: C16B1_PICSI (Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 8.113e-40
Identity = 75/143 (52.45%), Postives = 102/143 (71.33%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            + LE   ASP QDLLS LL   D  G  +++ EI DN+LLLL+AGHDTS S +++++K+L   P  YE+VL+EQL I+  KEEG+LL+WED+Q+MKYSW VA E LRL P + G++R A+K+  Y  +TIP  WKLHW   ++
Sbjct:  261 IALEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKLHWTVNST 403

[Close]

BLAST of GH720502 vs. SwissProt
Match: C16B2_PICSI (Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 4.452e-38
Identity = 72/143 (50.35%), Postives = 101/143 (70.63%), Query Frame = 1

Query:   79 VDLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNTRTS 507
            + LE   ASP QDLLS LL   D  G  +++ EI DN+LLLL+AGHDTS S +++++K+L   P  YE+VL+EQL+I+  KE G+LL+WED+Q+MKYSW VA E LRL P   G++R A+K+  Y  +TIP  WK++W   ++
Sbjct:  261 IGLEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGFTIPKGWKMYWTVNST 403

[Close]

BLAST of GH720502 vs. SwissProt
Match: T5H_TAXCU (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 7.355e-33
Identity = 69/139 (49.64%), Postives = 92/139 (66.19%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNT 498
            DL+   A+ TQDLLS LL   D  G  ++  EILDN   LL A +DT+ S ++LI K L + PE Y++V++EQLEI   KEEGE + W+D++ MKY+W VA E LR+ PP+ G +R A+ D+ Y  YTIP  WKL W T
Sbjct:  264 DLQSGSATATQDLLSVLLTFRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYTIPKGWKLLWTT 402

[Close]

BLAST of GH720502 vs. SwissProt
Match: T13H_TAXCU (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 9.941e-30
Identity = 63/139 (45.32%), Postives = 89/139 (64.03%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNT 498
            +L    AS  QDLLS LL  +D  G  +++ EILDN  +LL   +D++ S +++++K L + PE YE+V +EQ  I   K EGE + W+D+++MKYSW V  E LR+ PPI G +R A+ D+ Y  YTIP  WKL W T
Sbjct:  250 ELRAGTASENQDLLSVLLTFTDERGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLASHPESYEKVAQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITDIHYNGYTIPKGWKLLWTT 388

[Close]

BLAST of GH720502 vs. SwissProt
Match: T10H_TAXCU (Taxane 10-beta-hydroxylase OS=Taxus cuspidata GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 6.668e-26
Identity = 58/135 (42.96%), Postives = 84/135 (62.22%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            DL    AS  QDLLS LL   D  G  +++  ILDN   +  A +DT+ + ++LI K L + PE +E+V +EQLEI   K+EGE + W+D++ MKY+W    E LR+ PP+ G +R A+ D+ Y  YTIP  W++
Sbjct:  262 DLRSGIASDDQDLLSVLLTFRDEKGNSLTDQGILDNFSAMFHASYDTTVAPMALIFKLLYSNPEYHEKVFQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGIFRKAITDIHYDGYTIPKGWRV 396

[Close]

BLAST of GH720502 vs. SwissProt
Match: ABAH3_ARATH (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.031e-18
Identity = 49/136 (36.03%), Postives = 84/136 (61.76%), Query Frame = 1

Query:   85 LEEKKASPTQ--DLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            L +++ +P+   DLL   +   D +G  +++ +I DN++ ++FA  DT+ S ++ I+KYL + P V E V +EQ+ I + K+EGE L WED +KM  ++ V  E LR +  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  236 LSKRRQNPSSHTDLLGSFM--EDKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. SwissProt
Match: ABAH1_ARATH (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.117e-18
Identity = 48/121 (39.67%), Postives = 71/121 (58.68%), Query Frame = 1

Query:  142 SDTSGRFMSEVE------ILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            +D  G FM + E      I DN++ ++FA  DT+ S +S I+KYL   P V E V +EQ+ I + KEEGE L W D +KM  +  V  E LR++  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  247 NDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. SwissProt
Match: C90B1_ARATH (Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CYP90B1 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.658e-17
Identity = 53/133 (39.85%), Postives = 80/133 (60.15%), Query Frame = 1

Query:   94 KKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEE-GEL-LQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            +K     DLL  +L  S+     +S  +ILD +L LLFAGH+TS  AI+L + +L   P+  E++ +E LEI++ K+E GE  L W+D +KM ++  V +E LRL   +   +R A+KD+ Y  Y IP  WK+
Sbjct:  278 RKQRTDDDLLGWVLKHSN-----LSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKV 405

[Close]

BLAST of GH720502 vs. SwissProt
Match: ABAH1_ORYSJ (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. japonica GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 4.790e-16
Identity = 43/120 (35.83%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  133 LVTSDTSGR-FMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKE-EGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            L+ S   GR  +++ +I DN++ ++FA  DT+ S ++ ++K+LG+ P V + V +EQL+I++ KE  GE L W D ++MK +  V  E +R++  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  252 LLGSFVDGREALTDAQIADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEKEASGEPLSWADTRRMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKV 371

[Close]

BLAST of GH720502 vs. SwissProt
Match: ABAH1_ORYSI (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 4.790e-16
Identity = 43/120 (35.83%), Postives = 77/120 (64.17%), Query Frame = 1

Query:  133 LVTSDTSGR-FMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKE-EGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            L+ S   GR  +++ +I DN++ ++FA  DT+ S ++ ++K+LG+ P V + V +EQL+I++ KE  GE L W D ++MK +  V  E +R++  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  252 LLGSFVDGREALTDAQIADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEKEASGEPLSWADTRRMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKV 371

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT5G36110.1 (| Symbols: CYP716A1 | cytochrome P450, family 716, subfamily A, polypeptide 1 | chr5:14195377-14197613 FORWARD LENGTH=477)

HSP 1 Score: 121.324 bits (303), Expect = 2.990e-28
Identity = 55/138 (39.86%), Postives = 90/138 (65.22%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWN 495
            +L+  KA    D+LSH+L+    +     + ++ D ++ LL  GHDT+    + ++ YL   P VY++VL+EQ EI + K+E E L+WED++KM+YSWNVA EV+R+ PP++G +R A+   ++  + IP  WKL+W+
Sbjct:  249 ELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWS 382

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT2G42850.1 (| Symbols: CYP718 | cytochrome P450, family 718 | chr2:17831662-17833309 FORWARD LENGTH=485)

HSP 1 Score: 118.627 bits (296), Expect = 1.938e-27
Identity = 61/139 (43.88%), Postives = 85/139 (61.15%), Query Frame = 1

Query:   82 DLEEKKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKLHWNT 498
            ++E++ A      L   LV     G  ++E E++DNM+LL+FA HDT+  A+S+  K L   P   + +L+E  +I   K EGE L  EDV+KMKYSW V  E +RLSPPI G++R A+ D+ Y  YTIP  WK+ W T
Sbjct:  255 EMEKEGAEKPNTTLFSRLVEELIKG-VITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTT 392

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT5G45340.2 (| Symbols: CYP707A3 | cytochrome P450, family 707, subfamily A, polypeptide 3 | chr5:18369121-18370909 REVERSE LENGTH=446)

HSP 1 Score: 92.8189 bits (229), Expect = 1.139e-19
Identity = 49/136 (36.03%), Postives = 84/136 (61.76%), Query Frame = 1

Query:   85 LEEKKASPTQ--DLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            L +++ +P+   DLL   +   D +G  +++ +I DN++ ++FA  DT+ S ++ I+KYL + P V E V +EQ+ I + K+EGE L WED +KM  ++ V  E LR +  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  236 LSKRRQNPSSHTDLLGSFM--EDKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT5G45340.1 (| Symbols: CYP707A3 | cytochrome P450, family 707, subfamily A, polypeptide 3 | chr5:18368977-18370909 REVERSE LENGTH=463)

HSP 1 Score: 92.8189 bits (229), Expect = 1.139e-19
Identity = 49/136 (36.03%), Postives = 84/136 (61.76%), Query Frame = 1

Query:   85 LEEKKASPTQ--DLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            L +++ +P+   DLL   +   D +G  +++ +I DN++ ++FA  DT+ S ++ I+KYL + P V E V +EQ+ I + K+EGE L WED +KM  ++ V  E LR +  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  236 LSKRRQNPSSHTDLLGSFM--EDKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT4G19230.2 (| Symbols: CYP707A1 | cytochrome P450, family 707, subfamily A, polypeptide 1 | chr4:10521524-10523566 FORWARD LENGTH=484)

HSP 1 Score: 90.5077 bits (223), Expect = 5.652e-19
Identity = 48/121 (39.67%), Postives = 71/121 (58.68%), Query Frame = 1

Query:  142 SDTSGRFMSEVE------ILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            +D  G FM + E      I DN++ ++FA  DT+ S +S I+KYL   P V E V +EQ+ I + KEEGE L W D +KM  +  V  E LR++  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  247 NDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT4G19230.1 (| Symbols: CYP707A1 | cytochrome P450, family 707, subfamily A, polypeptide 1 | chr4:10521524-10523517 FORWARD LENGTH=467)

HSP 1 Score: 90.5077 bits (223), Expect = 5.652e-19
Identity = 48/121 (39.67%), Postives = 71/121 (58.68%), Query Frame = 1

Query:  142 SDTSGRFMSEVE------ILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            +D  G FM + E      I DN++ ++FA  DT+ S +S I+KYL   P V E V +EQ+ I + KEEGE L W D +KM  +  V  E LR++  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  247 NDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKV 367

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT3G50660.1 (| Symbols: DWF4, CYP90B1, CLM, SNP2, SAV1, PSC1 | Cytochrome P450 superfamily protein | chr3:18814262-18817168 REVERSE LENGTH=513)

HSP 1 Score: 87.0409 bits (214), Expect = 6.249e-18
Identity = 53/133 (39.85%), Postives = 80/133 (60.15%), Query Frame = 1

Query:   94 KKASPTQDLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEE-GEL-LQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            +K     DLL  +L  S+     +S  +ILD +L LLFAGH+TS  AI+L + +L   P+  E++ +E LEI++ K+E GE  L W+D +KM ++  V +E LRL   +   +R A+KD+ Y  Y IP  WK+
Sbjct:  278 RKQRTDDDLLGWVLKHSN-----LSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKV 405

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT2G29090.2 (| Symbols: CYP707A2 | cytochrome P450, family 707, subfamily A, polypeptide 2 | chr2:12495038-12499080 REVERSE LENGTH=482)

HSP 1 Score: 82.8037 bits (203), Expect = 1.179e-16
Identity = 43/124 (34.68%), Postives = 72/124 (58.06%), Query Frame = 1

Query:  118 LLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQG-KEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            LL  LL   D     +S+ +I DN++ ++FA  DT+ S ++ ++KYL + P + ++V +EQ  I Q  K+E   + WED +KM  +  V  E LR +  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  264 LLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKV 387

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT2G29090.1 (| Symbols: CYP707A2 | cytochrome P450, family 707, subfamily A, polypeptide 2 | chr2:12495038-12499080 REVERSE LENGTH=482)

HSP 1 Score: 82.8037 bits (203), Expect = 1.179e-16
Identity = 43/124 (34.68%), Postives = 72/124 (58.06%), Query Frame = 1

Query:  118 LLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQG-KEEGELLQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDWKL 486
            LL  LL   D     +S+ +I DN++ ++FA  DT+ S ++ ++KYL + P + ++V +EQ  I Q  K+E   + WED +KM  +  V  E LR +  ++  +R A++D+ Y  Y IP  WK+
Sbjct:  264 LLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKV 387

[Close]

BLAST of GH720502 vs. TAIR peptide
Match: AT1G12740.2 (| Symbols: CYP87A2 | cytochrome P450, family 87, subfamily A, polypeptide 2 | chr1:4342462-4344569 FORWARD LENGTH=478)

HSP 1 Score: 81.2629 bits (199), Expect = 3.429e-16
Identity = 41/136 (30.15%), Postives = 79/136 (58.09%), Query Frame = 1

Query:   85 LEEKKASPTQ---DLLSHLLVTSDTSGRFMSEVEILDNMLLLLFAGHDTSRSAISLIMKYLGNLPEVYEQVLKEQLEISQGKEEGEL-LQWEDVQKMKYSWNVASEVLRLSPPINGAYRNAMKDLTYADYTIPIDW 480
            L+E++ +P +   D   +++      G  ++E   LD M +LLFA  +T+  A++L +K+L + PEV +++ +E   I + +E+ +  L WE+ + M Y++   +E  RL+  +   +R A++D+ + DYTIP  W
Sbjct:  242 LQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIKFKDYTIPAGW 377

The following BLAST results are available for this feature:

BLAST of GH720502 vs. TrEMBL
BLAST of GH720502 vs. SwissProt
BLAST of GH720502 vs. TAIR peptide

BLAST of GH720502 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GLC6_POPTR	4.998e-49	60.39	Cytochrome P450 OS=Populus trichocarpa GN=CYP716D2...	[more]
B8Y882_STERE	6.110e-47	64.03	Cytochrome P450 monooxygenase (Fragment) OS=Stevia...	[more]
B9GLC4_POPTR	8.823e-46	60.28	Cytochrome P450 OS=Populus trichocarpa GN=CYP716D1...	[more]
B9GLC5_POPTR	5.719e-45	56.69	Cytochrome P450 OS=Populus trichocarpa GN=CYP716D2...	[more]
Q0NZP1_STERE	2.173e-44	61.87	Ent-kaurenoic acid 13-hydroxylase OS=Stevia rebaud...	[more]
B9RHG4_RICCO	6.323e-44	55.63	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
B9SPT3_RICCO	1.409e-43	55.94	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
B9HBL7_POPTR	1.409e-43	50.62	Cytochrome P450 OS=Populus trichocarpa GN=CYP716A8...	[more]
B9S060_RICCO	1.840e-43	71.67	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
B9SAB8_RICCO	9.130e-43	54.55	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]

BLAST of GH720502 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
C16B1_PICSI	8.113e-40	52.45	Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP71...	[more]
C16B2_PICSI	4.452e-38	50.35	Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP71...	[more]
T5H_TAXCU	7.355e-33	49.64	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata P...	[more]
T13H_TAXCU	9.941e-30	45.32	Taxane 13-alpha-hydroxylase OS=Taxus cuspidata GN=...	[more]
T10H_TAXCU	6.668e-26	42.96	Taxane 10-beta-hydroxylase OS=Taxus cuspidata GN=C...	[more]
ABAH3_ARATH	1.031e-18	36.03	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thal...	[more]
ABAH1_ARATH	5.117e-18	39.67	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thal...	[more]
C90B1_ARATH	5.658e-17	39.85	Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CY...	[more]
ABAH1_ORYSJ	4.790e-16	35.83	Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa sub...	[more]
ABAH1_ORYSI	4.790e-16	35.83	Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa sub...	[more]

BLAST of GH720502 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G36110.1	2.990e-28	39.86	\| Symbols: CYP716A1 \| cytochrome P450, family 716,...	[more]
AT2G42850.1	1.938e-27	43.88	\| Symbols: CYP718 \| cytochrome P450, family 718 \| ...	[more]
AT5G45340.2	1.139e-19	36.03	\| Symbols: CYP707A3 \| cytochrome P450, family 707,...	[more]
AT5G45340.1	1.139e-19	36.03	\| Symbols: CYP707A3 \| cytochrome P450, family 707,...	[more]
AT4G19230.2	5.652e-19	39.67	\| Symbols: CYP707A1 \| cytochrome P450, family 707,...	[more]
AT4G19230.1	5.652e-19	39.67	\| Symbols: CYP707A1 \| cytochrome P450, family 707,...	[more]
AT3G50660.1	6.249e-18	39.85	\| Symbols: DWF4, CYP90B1, CLM, SNP2, SAV1, PSC1 \| ...	[more]
AT2G29090.2	1.179e-16	34.68	\| Symbols: CYP707A2 \| cytochrome P450, family 707,...	[more]
AT2G29090.1	1.179e-16	34.68	\| Symbols: CYP707A2 \| cytochrome P450, family 707,...	[more]
AT1G12740.2	3.429e-16	30.15	\| Symbols: CYP87A2 \| cytochrome P450, family 87, s...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 9..166 score: 1.1
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 8..167 score: 1.8
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 9..159 score: 2.6
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 8..166 score: 1.9
None	No IPR available	PANTHER	PTHR19383:SF25	CYTOCHROME P450	coord: 8..167 score: 1.8
None	No IPR available	SEG	seg	seg	coord: 14..26 score
None	No IPR available	SEG	seg	seg	coord: 65..81 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720502 ID=GH720502; Name=GH720502; organism=Pisum sativum; type=EST; length=520bp
TTCGAGCGGCCGCCCGGGCAGGTGAAAGCTGCAGATGTAATAAAGAAAGA
AATTAAATTGATTATGAAGAAAAGGAAAGTGGATTTGGAAGAAAAGAAAG
CATCACCTACACAAGACCTTTTATCACATTTACTTGTTACATCTGACACA
AGTGGAAGGTTTATGAGTGAAGTGGAGATTCTTGATAACATGTTACTACT
TCTTTTCGCCGGCCATGACACTTCCAGATCAGCAATATCATTGATCATGA
AGTATCTTGGAAATTTGCCTGAAGTTTATGAACAAGTGTTAAAAGAACAA
CTTGAAATTAGCCAAGGGAAAGAGGAAGGAGAATTATTACAATGGGAGGA
TGTTCAAAAAATGAAATACTCATGGAATGTTGCATCTGAAGTTTTGAGGT
TGTCACCGCCAATCAACGGCGCCTACAGAAATGCTATGAAGGATTTGACC
TATGCTGATTATACCATCCCTATTGATTGGAAGTTGCATTGGAACACTCG
TACCTCGGCCGCGACCACGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

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