FG529355

EST Overview


Name	FG529355
Unique Name	FG529355
Type	EST
Organism	Pisum sativum (pea)
Sequence length	595

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG529355 vs. TrEMBL
Match: B9SP14_RICCO (GMP synthase, putative OS=Ricinus communis GN=RCOM_1247670 PE=4 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 6.222e-77
Identity = 142/152 (93.42%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVRQLGRI+ VPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYD IWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYF+HKFL+DV+ KICN V GVNRVV DITSKPPSTIEWE
Sbjct:  202 PLKLLFKDEVRQLGRILYVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDTIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLDDVARKICNSVRGVNRVVQDITSKPPSTIEWE 353

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BLAST of FG529355 vs. TrEMBL
Match: B9SBB6_RICCO (GMP synthase, putative OS=Ricinus communis GN=RCOM_0651320 PE=4 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.386e-76
Identity = 140/152 (92.11%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVRQLGR+M+VPEGFLKRHPFPGPGLA+RVLGDVTEGNALDILRQVDEIFIQ+IKDAGLYD IWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYF+HKFL DV+ KICN V GVNRVV DITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRQLGRMMNVPEGFLKRHPFPGPGLAIRVLGDVTEGNALDILRQVDEIFIQAIKDAGLYDTIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLADVARKICNRVRGVNRVVQDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TrEMBL
Match: B9GWS1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1074034 PE=4 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 4.033e-76
Identity = 140/152 (92.11%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVRQLGRI++VP+ FLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYD IWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYF+HKFL+DV+ KICN V GVNRVV DITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDSIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLDDVARKICNSVRGVNRVVQDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TrEMBL
Match: Q9CAD1_ARATH (At1g63660 OS=Arabidopsis thaliana GN=At1g63660 PE=2 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 6.438e-74
Identity = 135/152 (88.82%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LGRI++VP GFLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIFIQSI+DAGLYD IWQAFAVFLPVRSVGVQGD+RTHSHVVALRAVTSQDGMTADW+ F+HKFL+DVS KICN V GVNRVVLDITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVALRAVTSQDGMTADWFNFEHKFLDDVSRKICNSVQGVNRVVLDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TrEMBL
Match: Q84WB8_ARATH (Putative GMP synthase OS=Arabidopsis thaliana GN=At1g63660 PE=2 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 6.438e-74
Identity = 135/152 (88.82%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LGRI++VP GFLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIFIQSI+DAGLYD IWQAFAVFLPVRSVGVQGD+RTHSHVVALRAVTSQDGMTADW+ F+HKFL+DVS KICN V GVNRVVLDITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVALRAVTSQDGMTADWFNFEHKFLDDVSRKICNSVQGVNRVVLDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TrEMBL
Match: D7TVY8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_25.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019748001 PE=4 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 1.098e-73
Identity = 135/152 (88.82%), Postives = 144/152 (94.74%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LGRI++VP  FL+RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAG+YD IWQAFAVFLP+RSVGVQGDQRTHSHVVALRAVTSQDGMTADWY F+HKFL+DV+ KICN V GVNRVV DITSKPPSTIEWE
Sbjct:  342 PLKLLFKDEVRELGRILNVPRAFLQRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKPPSTIEWE 493

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BLAST of FG529355 vs. TrEMBL
Match: D7KTF3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893078 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.195e-73
Identity = 134/152 (88.16%), Postives = 145/152 (95.39%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LGRI++VP GFLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIFIQSI+DAGLYD IWQAFAVFLPVRSVGVQGD+RTHSHVVALRAVTSQDGMTADW+ F+HKFL+DVS KICN V GVNRVV DITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVALRAVTSQDGMTADWFNFEHKFLDDVSRKICNSVQGVNRVVQDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TrEMBL
Match: Q8H4X9_ORYSJ (Os08g0326600 protein OS=Oryza sativa subsp. japonica GN=OJ1136_A10.112 PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 2.799e-69
Identity = 124/152 (81.58%), Postives = 141/152 (92.76%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LG I++VPE FLKRHPFPGPGLAVRVLGDVTEGNAL++LRQVDEIF+Q+IKDAGLYD+IWQAFAVFLPV++VGVQGDQRTHS+ VALRA+TS+DGMTADWYYF+ +FL DV  KICN V GVNRVV DIT KPP+T+EWE
Sbjct:  393 PLKLLFKDEVRKLGSILNVPESFLKRHPFPGPGLAVRVLGDVTEGNALEVLRQVDEIFVQAIKDAGLYDIIWQAFAVFLPVQTVGVQGDQRTHSNAVALRAITSEDGMTADWYYFEREFLVDVVNKICNNVRGVNRVVQDITQKPPATVEWE 544

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BLAST of FG529355 vs. TrEMBL
Match: C5YB33_SORBI (Putative uncharacterized protein Sb06g033930 OS=Sorghum bicolor GN=Sb06g033930 PE=4 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 1.814e-68
Identity = 122/152 (80.26%), Postives = 139/152 (91.45%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LG I++VP+ FLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIF+Q+IKDAGLYD IWQAFAVFLPV++VGVQGDQRTHS+ V LRA+TS+DGMTADWYYFDH FL DV  KICN V G+NRV  DITSKPP+T+EWE
Sbjct:  394 PLKLLFKDEVRKLGSILNVPDSFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFVQAIKDAGLYDKIWQAFAVFLPVQTVGVQGDQRTHSNAVVLRAITSEDGMTADWYYFDHAFLVDVVNKICNNVRGINRVCQDITSKPPATVEWE 545

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BLAST of FG529355 vs. TrEMBL
Match: C0PH60_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 3.095e-68
Identity = 122/152 (80.26%), Postives = 139/152 (91.45%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LG I++VP+ FLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIF+QSIKDAGLYD IWQAFAVFLPV++VGVQGDQRTHS+ V LRA+TS+DGMTADWYYF+H FL DV  KICN V G+NRV  DITSKPP+T+EWE
Sbjct:  394 PLKLLFKDEVRKLGGILNVPDSFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFVQSIKDAGLYDKIWQAFAVFLPVQTVGVQGDQRTHSNAVVLRAITSEDGMTADWYYFEHAFLVDVVNKICNNVRGINRVCQDITSKPPATVEWE 545

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BLAST of FG529355 vs. SwissProt
Match: GUAA_THEM4 (GMP synthase [glutamine-hydrolyzing] OS=Thermosipho melanesiensis (strain BI429 / DSM 12029) GN=guaA PE=3 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 1.114e-52
Identity = 97/152 (63.82%), Postives = 122/152 (80.26%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR++G I+++P+  + RHPFPGPGLAVR++G VTE  A+ IL++ D IFI+ +K   LYD +WQAFAVFLP++SVGV GD RT+ +V+ALRAV S DGMTADW    H+FLN V+ +I NEV GVNRVV DITSKPP+TIEWE
Sbjct:  363 PLRYLFKDEVRKIGEILELPQSMINRHPFPGPGLAVRIIGKVTE-EAISILQKADYIFIEELKKNNLYDKVWQAFAVFLPIKSVGVMGDYRTYENVIALRAVNSFDGMTADWSKLPHEFLNKVAKRIINEVKGVNRVVYDITSKPPATIEWE 513

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BLAST of FG529355 vs. SwissProt
Match: GUAA_GEMAT (GMP synthase [glutamine-hydrolyzing] OS=Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505) GN=guaA PE=3 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 2.481e-52
Identity = 101/152 (66.45%), Postives = 124/152 (81.58%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR +GR + +PE  + RHPFPGPGLA+RVLG+VT  +ALD+LR+ D I+++ I+ AGLY  IWQAFAVFLPV+SVGV GD RT+ +VVALRAVTS DGMTADWY F ++ L  +S +I NEV GVNRVV DI+SKPP+TIEWE
Sbjct:  363 PLRELFKDEVRNVGRELGLPEEMVGRHPFPGPGLAIRVLGEVTP-HALDVLRRSDAIYLEEIRAAGLYPDIWQAFAVFLPVKSVGVMGDGRTYENVVALRAVTSTDGMTADWYPFPYEVLGRISNRIINEVDGVNRVVYDISSKPPATIEWE 513

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BLAST of FG529355 vs. SwissProt
Match: GUAA_BRAJA (GMP synthase [glutamine-hydrolyzing] OS=Bradyrhizobium japonicum GN=guaA PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 3.241e-52
Identity = 100/152 (65.79%), Postives = 121/152 (79.61%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR+LGR + +PE F+ RHPFPGPGLA+R  GD+T  + LDILR+ D ++I  I+  GLYD IWQAFAV LPV++VGV GD RT+ +VV LRAVTS DGMTAD+Y FD KFL + +T+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  401 PLRELFKDEVRKLGRELGLPEIFVGRHPFPGPGLAIRCPGDITS-DKLDILRKADAVYIDQIRKHGLYDDIWQAFAVLLPVKTVGVMGDGRTYDYVVGLRAVTSTDGMTADFYQFDMKFLGETATRIINEVKGVNRVVYDVTSKPPGTIEWE 551

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BLAST of FG529355 vs. SwissProt
Match: GUAA_OLICO (GMP synthase [glutamine-hydrolyzing] OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=guaA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 7.219e-52
Identity = 100/152 (65.79%), Postives = 120/152 (78.95%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +P+ F+ RHPFPGPGLA+R  G++T    LDILRQ D I+I+ I+ AGLYD IWQAFAV LPV++VGV GD RT+  VV LRAVTS DGMTAD+Y FD +FL + +T+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  381 PLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRCPGEITP-EKLDILRQADAIYIEEIRRAGLYDTIWQAFAVLLPVKTVGVMGDGRTYEFVVGLRAVTSTDGMTADFYPFDMRFLGETATRIINEVKGVNRVVYDVTSKPPGTIEWE 531

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BLAST of FG529355 vs. SwissProt
Match: GUAA_NITWN (GMP synthase [glutamine-hydrolyzing] OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=guaA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 7.219e-52
Identity = 100/152 (65.79%), Postives = 119/152 (78.29%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +PE F+ RHPFPGPGLA+R  G++T    LDILR+ D ++I  I+ AGLYD IWQAFAV LPV++VGV GD RT+ +VV LRAVTS DGMTAD+Y FD +FL   ST+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  385 PLRELFKDEVRALGRELGLPEIFVGRHPFPGPGLAIRCPGEITN-EKLDILREADAVYIDEIRKAGLYDNIWQAFAVLLPVKTVGVMGDSRTYDYVVGLRAVTSTDGMTADFYPFDAQFLGSTSTRIINEVKGVNRVVYDVTSKPPGTIEWE 535

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BLAST of FG529355 vs. SwissProt
Match: GUAA_BRASO (GMP synthase [glutamine-hydrolyzing] OS=Bradyrhizobium sp. (strain ORS278) GN=guaA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 7.219e-52
Identity = 100/152 (65.79%), Postives = 121/152 (79.61%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +P+ F+ RHPFPGPGLA+R  G++T  + LDILR+ D I+I  I+ AGLYD IWQAFAV LPV++VGV GD RT+ +VV LRAVTS DGMTAD+Y FD KFL + +T+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  385 PLRELFKDEVRVLGRELGLPDVFVGRHPFPGPGLAIRCPGEITT-DKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVVGLRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE 535

[Close]

BLAST of FG529355 vs. SwissProt
Match: GUAA_THEAB (GMP synthase [glutamine-hydrolyzing] OS=Thermosipho africanus (strain TCF52B) GN=guaA PE=3 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 9.429e-52
Identity = 96/152 (63.16%), Postives = 120/152 (78.95%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            P + LFKDEVR++G+I+ +P+  + RHPFPGPGLAVR++G+VT   A+ IL+  D IFI+ +K   LYD +WQAFAVFLP+RSVGV GD RT+ +V+ALRAV S DGMTADW    H+FLN V+ +I NEV GVNRVV DITSKPP+TIEWE
Sbjct:  363 PFRYLFKDEVRKIGKILGLPDEMINRHPFPGPGLAVRIIGEVTN-EAIKILQHADHIFIEELKKNDLYDKVWQAFAVFLPIRSVGVMGDYRTYDNVIALRAVNSFDGMTADWSKLPHEFLNKVAKRIVNEVDGVNRVVYDITSKPPATIEWE 513

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BLAST of FG529355 vs. SwissProt
Match: GUAA_RHORT (GMP synthase [glutamine-hydrolyzing] OS=Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) GN=guaA PE=3 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 9.429e-52
Identity = 98/152 (64.47%), Postives = 118/152 (77.63%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +P+  + RHPFPGPGLA+R+ G       LDILR+ D I+++ I++AGLYDVIWQAFAV LPVR+VGV GD R++   +ALRAVTS DGMTAD+Y FDH FL  V+ +I NEV GVNRVV DITSKPP TIEWE
Sbjct:  374 PLRELFKDEVRDLGRELGLPDEMVGRHPFPGPGLAIRIPGQPLTREKLDILRRADAIYLEEIRNAGLYDVIWQAFAVLLPVRTVGVMGDARSYDFALALRAVTSTDGMTADYYPFDHTFLGRVANRIINEVKGVNRVVYDITSKPPGTIEWE 525

[Close]

BLAST of FG529355 vs. SwissProt
Match: GUAA_RHOPA (GMP synthase [glutamine-hydrolyzing] OS=Rhodopseudomonas palustris GN=guaA PE=3 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 1.231e-51
Identity = 100/152 (65.79%), Postives = 119/152 (78.29%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +P+ F+ RHPFPGPGLA+R  G++TE   L+ILR  D ++I  I+ AGLYDVIWQAFAV LPVRSVGV GD RT+ +VV LRAVTS DGMTAD+Y F+  FL   +T+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  390 PLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRCPGEITE-EKLEILRNADAVYIDQIRKAGLYDVIWQAFAVLLPVRSVGVMGDGRTYDYVVGLRAVTSTDGMTADFYPFEMSFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE 540

[Close]

BLAST of FG529355 vs. SwissProt
Match: GUAA_RHOP2 (GMP synthase [glutamine-hydrolyzing] OS=Rhodopseudomonas palustris (strain HaA2) GN=guaA PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.679e-51
Identity = 99/152 (65.13%), Postives = 117/152 (76.97%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PL+ LFKDEVR LGR + +PE F+ RHPFPGPGLA+R  G++T    LDILR  D ++I  I+ AGLYD IWQAFAV LPV++VGV GD RT+ +VV LRAVTS DGMTAD+Y FD  FL   +T+I NEV GVNRVV D+TSKPP TIEWE
Sbjct:  390 PLRELFKDEVRALGRELGLPEIFVGRHPFPGPGLAIRCPGEIT-AEKLDILRNADAVYIDQIRKAGLYDAIWQAFAVLLPVKTVGVMGDGRTYEYVVGLRAVTSTDGMTADYYPFDMAFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWE 540

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BLAST of FG529355 vs. TAIR peptide
Match: AT1G63660.1 (| Symbols: | GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, putative | chr1:23604127-23607080 REVERSE LENGTH=534)

HSP 1 Score: 280.411 bits (716), Expect = 4.879e-76
Identity = 135/152 (88.82%), Postives = 146/152 (96.05%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDVIWQAFAVFLPVRSVGVQGDQRTHSHVVALRAVTSQDGMTADWYYFDHKFLNDVSTKICNEVVGVNRVVLDITSKPPSTIEWE 458
            PLKLLFKDEVR+LGRI++VP GFLKRHPFPGPGLAVRVLGDVT+GNAL++LRQVDEIFIQSI+DAGLYD IWQAFAVFLPVRSVGVQGD+RTHSHVVALRAVTSQDGMTADW+ F+HKFL+DVS KICN V GVNRVVLDITSKPPSTIEWE
Sbjct:  383 PLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNALEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVALRAVTSQDGMTADWFNFEHKFLDDVSRKICNSVQGVNRVVLDITSKPPSTIEWE 534

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BLAST of FG529355 vs. TAIR peptide
Match: AT1G63660.2 (| Symbols: | GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, putative | chr1:23604874-23607080 REVERSE LENGTH=434)

HSP 1 Score: 98.2117 bits (243), Expect = 3.434e-21
Identity = 45/52 (86.54%), Postives = 51/52 (98.08%), Query Frame = 3

Query:    3 PLKLLFKDEVRQLGRIMDVPEGFLKRHPFPGPGLAVRVLGDVTEGNALDILR 158
            PLKLLFKDEVR+LGRI++VP GFLKRHPFPGPGLAVRVLGDVT+GNAL++LR
Sbjct:  383 PLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNALEVLR 434

The following BLAST results are available for this feature:

BLAST of FG529355 vs. TrEMBL
BLAST of FG529355 vs. SwissProt
BLAST of FG529355 vs. TAIR peptide

BLAST of FG529355 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SP14_RICCO	6.222e-77	93.42	GMP synthase, putative OS=Ricinus communis GN=RCOM...	[more]
B9SBB6_RICCO	1.386e-76	92.11	GMP synthase, putative OS=Ricinus communis GN=RCOM...	[more]
B9GWS1_POPTR	4.033e-76	92.11	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9CAD1_ARATH	6.438e-74	88.82	At1g63660 OS=Arabidopsis thaliana GN=At1g63660 PE=...	[more]
Q84WB8_ARATH	6.438e-74	88.82	Putative GMP synthase OS=Arabidopsis thaliana GN=A...	[more]
D7TVY8_VITVI	1.098e-73	88.82	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KTF3_ARALY	3.195e-73	88.16	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q8H4X9_ORYSJ	2.799e-69	81.58	Os08g0326600 protein OS=Oryza sativa subsp. japoni...	[more]
C5YB33_SORBI	1.814e-68	80.26	Putative uncharacterized protein Sb06g033930 OS=So...	[more]
C0PH60_MAIZE	3.095e-68	80.26	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of FG529355 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GUAA_THEM4	1.114e-52	63.82	GMP synthase [glutamine-hydrolyzing] OS=Thermosiph...	[more]
GUAA_GEMAT	2.481e-52	66.45	GMP synthase [glutamine-hydrolyzing] OS=Gemmatimon...	[more]
GUAA_BRAJA	3.241e-52	65.79	GMP synthase [glutamine-hydrolyzing] OS=Bradyrhizo...	[more]
GUAA_OLICO	7.219e-52	65.79	GMP synthase [glutamine-hydrolyzing] OS=Oligotroph...	[more]
GUAA_NITWN	7.219e-52	65.79	GMP synthase [glutamine-hydrolyzing] OS=Nitrobacte...	[more]
GUAA_BRASO	7.219e-52	65.79	GMP synthase [glutamine-hydrolyzing] OS=Bradyrhizo...	[more]
GUAA_THEAB	9.429e-52	63.16	GMP synthase [glutamine-hydrolyzing] OS=Thermosiph...	[more]
GUAA_RHORT	9.429e-52	64.47	GMP synthase [glutamine-hydrolyzing] OS=Rhodospiri...	[more]
GUAA_RHOPA	1.231e-51	65.79	GMP synthase [glutamine-hydrolyzing] OS=Rhodopseud...	[more]
GUAA_RHOP2	4.679e-51	65.13	GMP synthase [glutamine-hydrolyzing] OS=Rhodopseud...	[more]

BLAST of FG529355 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G63660.1	4.879e-76	88.82	\| Symbols: \| GMP synthase (glutamine-hydrolyzing)...	[more]
AT1G63660.2	3.434e-21	86.54	\| Symbols: \| GMP synthase (glutamine-hydrolyzing)...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001674	GMP synthase, C-terminal	PFAM	PF00958	GMP_synt_C	coord: 60..151 score: 3.3
None	No IPR available	GENE3D	3.30.300.10	no description	coord: 27..152 score: 6.8
None	No IPR available	PANTHER	PTHR11922	GMP SYNTHASE-RELATED	coord: 1..152 score: 1.2
None	No IPR available	PANTHER	PTHR11922:SF2	GMP SYNTHASE	coord: 1..152 score: 1.2
None	No IPR available	SUPERFAMILY	54810	GMP synthetase C-terminal dimerisation domain	coord: 31..152 score: 4.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529355 ID=FG529355; Name=FG529355; organism=Pisum sativum; type=EST; length=595bp
AACCACTTAAACTACTTTTCAAGGATGAGGTTCGTCAATTAGGGAGGATC
ATGGATGTTCCCGAGGGATTTCTAAAACGTCATCCCTTCCCTGGGCCTGG
TCTTGCAGTTAGAGTATTGGGTGATGTGACTGAAGGAAATGCGTTAGATA
TTCTCCGACAGGTTGATGAGATTTTCATTCAGTCAATCAAAGATGCTGGC
CTCTATGATGTAATATGGCAAGCCTTTGCAGTTTTCTTACCAGTAAGATC
TGTTGGAGTTCAAGGTGATCAAAGAACACACTCCCATGTTGTCGCACTCA
GAGCTGTTACAAGTCAAGATGGAATGACAGCAGACTGGTACTACTTTGAC
CACAAGTTCCTTAATGATGTCTCAACCAAAATCTGTAATGAGGTCGTTGG
TGTGAATCGTGTTGTGCTAGATATTACGTCAAAGCCTCCATCAACAATTG
AGTGGGAATAATATCAGTTAGTACTTTTATTTTTAAATGATTGTGGGGTG
CTATTCAGTTGTATAAGCGATAAAGTACTTTCTATTTACTTGTTTGAGTT
ATTGTTTTCCCTCTTGCCTCTAGGGCTTGGAGGGAAGGGGACTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003922	GMP synthase (glutamine-hydrolyzing) activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006164	purine nucleotide biosynthetic process
GO:0006177	GMP biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001674	GMP_synth_C

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