EX570067

EST Overview


Name	EX570067
Unique Name	EX570067
Type	EST
Organism	Pisum sativum (pea)
Sequence length	461

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570067 vs. TrEMBL
Match: B7FJ86_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 7.030e-31
Identity = 74/112 (66.07%), Postives = 86/112 (76.79%), Query Frame = 3

Query:    3 RVLHLDPSSTSFNDTRGVLGLNSLLPQLHAGAASGKVFISAFSSKTKEDKIESVPLPLGLPRIAVAAARPAFFGTRLKGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHTIA 338
            R L  DP+S    + RGV GLNSLLPQ+HAG++SGKV +SAFS K   D+ ESV   LGLPRIAVAA+RP FFG  +KG+ EKLEIRNWKK+SDVW +RNKRK AISW  IA
Sbjct:  103 RFLKHDPASNG--EIRGVFGLNSLLPQIHAGSSSGKVLLSAFSGKG--DESESVSSALGLPRIAVAASRPEFFG--VKGDGEKLEIRNWKKSSDVWMNRNKRKTAISWQNIA 208

[Close]

BLAST of EX570067 vs. TrEMBL
Match: C6T408_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.122e-28
Identity = 65/112 (58.04%), Postives = 82/112 (73.21%), Query Frame = 3

Query:    3 RVLHLDPSSTSFNDTRGVLGLNSLLPQLHAGAASGKVFISAFSSKTKEDKIESVPLPLGLPRIAVAAARPAFFGTRLKGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHTIA 338
            ++LHLDP++T  N  RGV  LNS+LP  HAG +S ++F+SA +SK K  + ESV   LG+PR AVAAAR  FF  R  GE E LE+R+W++NSD W HRNKRKAAISW +IA
Sbjct:   94 KLLHLDPAATDANSIRGVFALNSMLPHAHAGTSSRELFLSALASKAKGSESESVS-SLGIPRFAVAAARAPFFAQRAIGE-ETLEVRHWRRNSDAWAHRNKRKAAISWQSIA 203

[Close]

BLAST of EX570067 vs. TrEMBL
Match: Q40600_OENBE (70s mitochondrial ribosomal protein L2 OS=Oenothera bertiana GN=rp12 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.188e-11
Identity = 41/94 (43.62%), Postives = 55/94 (58.51%), Query Frame = 3

Query:   93 GAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRLKGEDEKL---------EIRNWKKNSDVWEHRNKRKAAISW 326
            GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R++ +++KL         E+R W+ +S +W HR KRKAA+SW
Sbjct:  151 GAGSKKTCAKDVFFSAFSSPKAKGETASLSFGSSFGFPRIAVAGAKPAFFAPRMREDEDKLRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSW 244

[Close]

BLAST of EX570067 vs. TrEMBL
Match: B9NCW6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_828828 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.238e-10
Identity = 41/92 (44.57%), Postives = 51/92 (55.43%), Query Frame = 3

Query:   93 GAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWH 329
            GA S K     VF SAFSS   + +  S+     L  PRIAVA A+PAFF  R+      K      E+R W+ +S +W HR KRKAA+SWH
Sbjct:  159 GAGSKKTCAKDVFFSAFSSPKAKGETASLSFGSSLSFPRIAVAGAKPAFFAPRMREKVRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSWH 250

[Close]

BLAST of EX570067 vs. TrEMBL
Match: Q5MF19_EICCR (Ribosomal protein L2 (Fragment) OS=Eichhornia crassipes GN=rpl2 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.841e-10
Identity = 41/102 (40.20%), Postives = 56/102 (54.90%), Query Frame = 3

Query:   54 VLGLNSLLPQLHAGAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRLKGEDEKL----EIRNWKKNSDVWEHRNKRKAAISW 326
            V+GL + +P     A S +     VF SA S    + +  S+     LG PRIAVA A+PAFF  R++   +K     EIR W+ +  +W HR KRKAA+SW
Sbjct:  115 VVGLETFMPPWSTCAGSKQTCAKDVFFSALSPPLAKGETASLSFGSSLGFPRIAVAGAKPAFFAPRMRENGKKTFSLCEIRKWRTHCVLWAHRIKRKAALSW 216

[Close]

BLAST of EX570067 vs. TrEMBL
Match: D5I3E9_CUCPE (Ribosomal protein L2 OS=Cucurbita pepo GN=rpl2 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.841e-10
Identity = 41/98 (41.84%), Postives = 53/98 (54.08%), Query Frame = 3

Query:   78 PQLHAGAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            P    GA S K     VF SAFSS+  + +  S+     LG PRIAVA A+PAFF  R+      K      E+R W+ +S +W HR KRKAA+ W +
Sbjct:  149 PFTSKGAGSKKTCAKDVFFSAFSSQKTKGETASLSFGSSLGFPRIAVAGAKPAFFAPRMREKVRGKNTFSLCEVRKWRTHSILWAHRMKRKAALDWQS 246

[Close]

BLAST of EX570067 vs. TrEMBL
Match: B6VJV4_VITVI (Ribosomal protein L2 OS=Vitis vinifera GN=rpl2 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.935e-10
Identity = 43/101 (42.57%), Postives = 55/101 (54.46%), Query Frame = 3

Query:   78 PQLHAGAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRLKGEDEKL---------EIRNWKKNSDVWEHRNKRKAAISWHT 332
            P    GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R++   EKL         E+R W+ +S +W HR KRKAA+SW +
Sbjct:  152 PFTSKGAGSKKTCAKDVFFSAFSSPKAKGETSSLSFGSSFGFPRIAVAGAKPAFFAPRMR---EKLRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSWQS 249

[Close]

BLAST of EX570067 vs. TrEMBL
Match: A5BK62_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_029794 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.935e-10
Identity = 43/101 (42.57%), Postives = 55/101 (54.46%), Query Frame = 3

Query:   78 PQLHAGAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRLKGEDEKL---------EIRNWKKNSDVWEHRNKRKAAISWHT 332
            P    GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R++   EKL         E+R W+ +S +W HR KRKAA+SW +
Sbjct:  152 PFTSKGAGSKKTCAKDVFFSAFSSPKAKGETSSLSFGSSFGFPRIAVAGAKPAFFAPRMR---EKLRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSWQS 249

[Close]

BLAST of EX570067 vs. TrEMBL
Match: D5I399_CITLA (Ribosomal protein L2 OS=Citrullus lanatus GN=rpl2 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.167e-9
Identity = 40/98 (40.82%), Postives = 53/98 (54.08%), Query Frame = 3

Query:   78 PQLHAGAASGK-----VFISAFSSKTKEDKIESVPL--PLGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            P    GA S K     VF SAFSS+  + +  S+      G PR+AVA A+PAFF  R+      K      E+R W+ +S +W HR KRKAA+SW +
Sbjct:  149 PFTSKGAGSKKTCAKDVFFSAFSSQKTKGETASLSFGSSFGFPRVAVAGAKPAFFAPRMRDKLRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSWRS 246

[Close]

BLAST of EX570067 vs. TrEMBL
Match: Q8WCM3_9ROSA (Ribosomal protein L2 (Fragment) OS=Ulmus thomasii GN=rpl2 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.600e-9
Identity = 46/109 (42.20%), Postives = 58/109 (53.21%), Query Frame = 3

Query:   54 VLGLNSLLPQ---LHAGAASGK-----VFISAFSS-KTKEDKIE-SVPLPLGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            V+GL + +P       GA S K     VF SAFSS KTK +    S     G PRIAVA A+PAF   R+      K      E+R W+ +S +W HR KRKAA+SW +
Sbjct:  113 VVGLPTRMPPSPFTSKGAGSKKTCAKDVFFSAFSSPKTKGETASLSFDSSFGFPRIAVAGAKPAFLAPRMREKVRGKNTFSLCEVRKWRTHSILWAHRIKRKAALSWQS 221

[Close]

BLAST of EX570067 vs. SwissProt
Match: RM02_ORYSJ (60S ribosomal protein L2, mitochondrial OS=Oryza sativa subsp. japonica GN=RPL2 PE=2 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 1.562e-8
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 3

Query:  174 LGLPRIAVAAARPAFFGTRLKGED------EKLEIRNWKKNSDVWEHRNKRKAAISWHTI 335
            LG PRIAVA A+PAFF  R++ +          EIR W+ +  +W HR KRKAA+SW ++
Sbjct:  134 LGFPRIAVAGAKPAFFAERMREKKIGKKTFSLCEIRKWRTHCVLWAHRIKRKAALSWQSL 193

[Close]

BLAST of EX570067 vs. SwissProt
Match: RM02_ORYSI (60S ribosomal protein L2, mitochondrial OS=Oryza sativa subsp. indica GN=RPL2 PE=2 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 1.562e-8
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 3

Query:  174 LGLPRIAVAAARPAFFGTRLKGED------EKLEIRNWKKNSDVWEHRNKRKAAISWHTI 335
            LG PRIAVA A+PAFF  R++ +          EIR W+ +  +W HR KRKAA+SW ++
Sbjct:  134 LGFPRIAVAGAKPAFFAERMREKKIGKKTFSLCEIRKWRTHCVLWAHRIKRKAALSWQSL 193

[Close]

BLAST of EX570067 vs. SwissProt
Match: RM02_ORYSA (60S ribosomal protein L2, mitochondrial OS=Oryza sativa GN=RPL2 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.562e-8
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 3

Query:  174 LGLPRIAVAAARPAFFGTRLKGED------EKLEIRNWKKNSDVWEHRNKRKAAISWHTI 335
            LG PRIAVA A+PAFF  R++ +          EIR W+ +  +W HR KRKAA+SW ++
Sbjct:  134 LGFPRIAVAGAKPAFFAERMREKKIGKKTFSLCEIRKWRTHCVLWAHRIKRKAALSWQSL 193

[Close]

BLAST of EX570067 vs. SwissProt
Match: RM02_ARATH (60S ribosomal protein L2, mitochondrial OS=Arabidopsis thaliana GN=RPL2 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.322e-7
Identity = 41/114 (35.96%), Postives = 57/114 (50.00%), Query Frame = 3

Query:   54 VLGLNSLLPQLHA--------GAASGK-----VFISAFSSKTKEDKIESVPLP--LGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            V+GL + +P L +        GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R+      K      E++  + +S +W HR K KA +SW +
Sbjct:  134 VVGLPTGMPPLSSSKSAFASKGAGSTKTLVKDVFFSAFSSPKAKRETASLAFASSFGFPRIAVAGAKPAFFAPRMRQKVRGKSTFSLCEVQKGRTHSILWAHRIKGKAGLSWQS 247

[Close]

BLAST of EX570067 vs. TAIR peptide
Match: ATMG00560.1 (| Symbols: RPL2 | Nucleic acid-binding, OB-fold-like protein | chrM:154744-157345 FORWARD LENGTH=349)

HSP 1 Score: 55.4546 bits (132), Expect = 1.541e-8
Identity = 41/114 (35.96%), Postives = 57/114 (50.00%), Query Frame = 3

Query:   54 VLGLNSLLPQLHA--------GAASGK-----VFISAFSSKTKEDKIESVPLP--LGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            V+GL + +P L +        GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R+      K      E++  + +S +W HR K KA +SW +
Sbjct:  134 VVGLPTGMPPLSSSKSAFASKGAGSTKTLVKDVFFSAFSSPKAKRETASLAFASSFGFPRIAVAGAKPAFFAPRMRQKVRGKSTFSLCEVQKGRTHSILWAHRIKGKAGLSWQS 247

[Close]

BLAST of EX570067 vs. TAIR peptide
Match: AT2G07715.1 (| Symbols: | Nucleic acid-binding, OB-fold-like protein | chr2:3411180-3412694 FORWARD LENGTH=389)

HSP 1 Score: 55.0694 bits (131), Expect = 2.013e-8
Identity = 41/114 (35.96%), Postives = 57/114 (50.00%), Query Frame = 3

Query:   54 VLGLNSLLPQLHA--------GAASGK-----VFISAFSSKTKEDKIESVPLP--LGLPRIAVAAARPAFFGTRL------KGEDEKLEIRNWKKNSDVWEHRNKRKAAISWHT 332
            V+GL + +P L +        GA S K     VF SAFSS   + +  S+      G PRIAVA A+PAFF  R+      K      E++  + +S +W HR K KA +SW +
Sbjct:  134 VVGLPTGMPPLSSSKSAFASKGAGSTKTLVKDVFFSAFSSLKAKRETASLAFASSFGFPRIAVAGAKPAFFAPRMRQKVRGKSTFSLCEVQKGRTHSILWAHRIKGKAGLSWQS 247

The following BLAST results are available for this feature:

BLAST of EX570067 vs. TrEMBL
BLAST of EX570067 vs. SwissProt
BLAST of EX570067 vs. TAIR peptide

BLAST of EX570067 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FJ86_MEDTR	7.030e-31	66.07	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6T408_SOYBN	1.122e-28	58.04	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q40600_OENBE	6.188e-11	43.62	70s mitochondrial ribosomal protein L2 OS=Oenother...	[more]
B9NCW6_POPTR	5.238e-10	44.57	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q5MF19_EICCR	6.841e-10	40.20	Ribosomal protein L2 (Fragment) OS=Eichhornia cras...	[more]
D5I3E9_CUCPE	6.841e-10	41.84	Ribosomal protein L2 OS=Cucurbita pepo GN=rpl2 PE=...	[more]
B6VJV4_VITVI	8.935e-10	42.57	Ribosomal protein L2 OS=Vitis vinifera GN=rpl2 PE=...	[more]
A5BK62_VITVI	8.935e-10	42.57	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D5I399_CITLA	1.167e-9	40.82	Ribosomal protein L2 OS=Citrullus lanatus GN=rpl2 ...	[more]
Q8WCM3_9ROSA	2.600e-9	42.20	Ribosomal protein L2 (Fragment) OS=Ulmus thomasii ...	[more]

BLAST of EX570067 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
RM02_ORYSJ	1.562e-8	46.67	60S ribosomal protein L2, mitochondrial OS=Oryza s...	[more]
RM02_ORYSI	1.562e-8	46.67	60S ribosomal protein L2, mitochondrial OS=Oryza s...	[more]
RM02_ORYSA	1.562e-8	46.67	60S ribosomal protein L2, mitochondrial OS=Oryza s...	[more]
RM02_ARATH	1.322e-7	35.96	60S ribosomal protein L2, mitochondrial OS=Arabido...	[more]

BLAST of EX570067 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
ATMG00560.1	1.541e-8	35.96	\| Symbols: RPL2 \| Nucleic acid-binding, OB-fold-li...	[more]
AT2G07715.1	2.013e-8	35.96	\| Symbols: \| Nucleic acid-binding, OB-fold-like p...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 39..47 score
None	No IPR available	PANTHER	PTHR13691:SF2	MITOCHONDRIAL 60S RIBOSOMAL PROTEIN L2	coord: 37..110 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 57..75 score
IPR002171	Ribosomal protein L2	PANTHER	PTHR13691	RIBOSOMAL PROTEIN L2	coord: 37..110 score: 4.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570067 ID=EX570067; Name=EX570067; organism=Pisum sativum; type=EST; length=461bp
CTAGAGTCCTCCATCTCGATCCATCGTCAACCTCCTTTAATGACACTCGC
GGCGTGCTTGGTTTGAACTCATTGCTTCCGCAACTCCACGCCGGAGCTGC
TTCCGGGAAGGTTTTCATTTCTGCGTTTTCGTCAAAGACTAAAGAAGATA
AAATTGAATCCGTTCCGTTGCCGTTAGGGTTACCGAGGATTGCTGTGGCG
GCGGCGAGACCTGCATTCTTTGGTACGCGGTTGAAAGGTGAGGATGAGAA
GTTGGAGATTCGTAATTGGAAGAAGAATAGTGATGTTTGGGAGCATAGAA
ACAAACGGAAAGCGGCGATTTCTTGGCATACCATTGCTTGATTGTAAAAT
AGAGTTTATCATTTTCATTTTCGAAAGACTAATTGTTGATTTAATTTTAG
TTGGAATTGAGTTTTTGCATGTTAAAGCATGATTCATAAATTGATGATAT
TTTAGGTTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR002171	Ribosomal_uL2

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