GH719505

EST Overview


Name	GH719505
Unique Name	GH719505
Type	EST
Organism	Pisum sativum (pea)
Sequence length	257

Libraries


Library Name	Type
LIBEST_024268 TI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719505 vs. TrEMBL
Match: B9T6V3_RICCO (Multidrug resistance pump, putative OS=Ricinus communis GN=RCOM_0063820 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.500e-12
Identity = 33/44 (75.00%), Postives = 37/44 (84.09%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKK 132
            IWAGMIFGGTA QTLIL  IT+RCDW+KEA KA +HV KWS+ K
Sbjct:  439 IWAGMIFGGTAIQTLILALITIRCDWDKEAEKASMHVKKWSEVK 482

[Close]

BLAST of GH719505 vs. TrEMBL
Match: D7UE71_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_477.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007071001 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.558e-12
Identity = 33/42 (78.57%), Postives = 36/42 (85.71%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IWAGMIFGGTA QTLIL  IT+RCDWEKEA KA LHV KW++
Sbjct:  390 IWAGMIFGGTALQTLILAIITIRCDWEKEAEKASLHVKKWAE 431

[Close]

BLAST of GH719505 vs. TrEMBL
Match: A5ACN2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009768 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.558e-12
Identity = 33/42 (78.57%), Postives = 36/42 (85.71%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IWAGMIFGGTA QTLIL  IT+RCDWEKEA KA LHV KW++
Sbjct:  445 IWAGMIFGGTALQTLILAIITIRCDWEKEAEKASLHVKKWAE 486

[Close]

BLAST of GH719505 vs. TrEMBL
Match: B9HFS0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_655493 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.443e-12
Identity = 33/42 (78.57%), Postives = 35/42 (83.33%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IWAGMIFGGTA QTLIL  IT+RCDWEKEA KA  HV KWS+
Sbjct:  445 IWAGMIFGGTAVQTLILAIITIRCDWEKEAEKASQHVLKWSE 486

[Close]

BLAST of GH719505 vs. TrEMBL
Match: D7MN36_ARALY (Mate efflux family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_494591 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.721e-12
Identity = 32/41 (78.05%), Postives = 36/41 (87.80%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWS 123
            IWAGMIFGGT  QTLIL +IT+RCDWEKEA KAK+ V+KWS
Sbjct:  444 IWAGMIFGGTMVQTLILIFITIRCDWEKEAQKAKVRVNKWS 484

[Close]

BLAST of GH719505 vs. TrEMBL
Match: D7MTX6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496771 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.658e-11
Identity = 31/42 (73.81%), Postives = 36/42 (85.71%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IWAGMIFGGTA QT+IL +IT+RCDWEKEA KA   V+KWS+
Sbjct:  442 IWAGMIFGGTAVQTMILAFITMRCDWEKEAQKANARVNKWSN 483

[Close]

BLAST of GH719505 vs. TrEMBL
Match: Q4ABZ9_BRACM (117M18_21 OS=Brassica campestris GN=117M18_21 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.166e-11
Identity = 31/41 (75.61%), Postives = 34/41 (82.93%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWS 123
            IWAGMIFGGT  QTLIL +I +RCDWEKEA KA +HV KWS
Sbjct:  385 IWAGMIFGGTGIQTLILIFIVMRCDWEKEAQKASVHVKKWS 425

[Close]

BLAST of GH719505 vs. TrEMBL
Match: Q9FNC1_ARATH (Emb|CAB89401.1 OS=Arabidopsis thaliana GN=At5g44050 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.694e-11
Identity = 31/41 (75.61%), Postives = 35/41 (85.37%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWS 123
            IWAGMIFGGT  QTLIL +IT+RCDWEKEA  AK+ V+KWS
Sbjct:  445 IWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

[Close]

BLAST of GH719505 vs. TrEMBL
Match: Q4ABZ7_BRACM (117M18_23 OS=Brassica campestris GN=117M18_23 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.824e-11
Identity = 31/41 (75.61%), Postives = 35/41 (85.37%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWS 123
            IWAGMIFGGTA QTLIL +I +RCDWEKEA KA + V+KWS
Sbjct:  470 IWAGMIFGGTAMQTLILIFIVMRCDWEKEAQKASVRVNKWS 510

[Close]

BLAST of GH719505 vs. TrEMBL
Match: Q9FKQ1_ARATH (AT5g65380/MNA5_11 OS=Arabidopsis thaliana GN=At5g65380 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.229e-11
Identity = 29/42 (69.05%), Postives = 35/42 (83.33%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IW GMIFGGTA QT+IL +IT+RCDWEKEA KA   ++KWS+
Sbjct:  442 IWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWSN 483

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT5G44050.1 (| Symbols: | MATE efflux family protein | chr5:17722484-17726209 FORWARD LENGTH=491)

HSP 1 Score: 71.2478 bits (173), Expect = 1.437e-13
Identity = 31/41 (75.61%), Postives = 35/41 (85.37%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWS 123
            IWAGMIFGGT  QTLIL +IT+RCDWEKEA  AK+ V+KWS
Sbjct:  445 IWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT5G65380.1 (| Symbols: | MATE efflux family protein | chr5:26123241-26126352 REVERSE LENGTH=486)

HSP 1 Score: 70.0922 bits (170), Expect = 3.201e-13
Identity = 29/42 (69.05%), Postives = 35/42 (83.33%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IW GMIFGGTA QT+IL +IT+RCDWEKEA KA   ++KWS+
Sbjct:  442 IWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWSN 483

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT5G10420.1 (| Symbols: | MATE efflux family protein | chr5:3273578-3276490 REVERSE LENGTH=489)

HSP 1 Score: 63.1586 bits (152), Expect = 3.913e-11
Identity = 27/40 (67.50%), Postives = 31/40 (77.50%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKW 120
            IWAGMIFGGTA QTLIL  IT RCDW+ EA K+ + + KW
Sbjct:  443 IWAGMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT3G21690.1 (| Symbols: | MATE efflux family protein | chr3:7638750-7641861 FORWARD LENGTH=506)

HSP 1 Score: 59.6918 bits (143), Expect = 4.326e-10
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKKQQL 141
            IW GMI GGT  QT IL W+T R DW KE  +A   + KWS+KKQ++
Sbjct:  458 IWTGMI-GGTVIQTFILAWVTFRTDWTKEVEEASKRLDKWSNKKQEV 503

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT4G21910.3 (| Symbols: | MATE efflux family protein | chr4:11625821-11630964 REVERSE LENGTH=507)

HSP 1 Score: 56.6102 bits (135), Expect = 3.662e-9
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKKQQL 141
            IW GMI GGT  QTLIL ++T R DW+KE  KA+  +  W DKK+ L
Sbjct:  460 IWTGMI-GGTLMQTLILLYVTYRTDWDKEVEKARKRLDLWDDKKEPL 505

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT4G21910.1 (| Symbols: | MATE efflux family protein | chr4:11625742-11630964 REVERSE LENGTH=507)

HSP 1 Score: 56.6102 bits (135), Expect = 3.662e-9
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKKQQL 141
            IW GMI GGT  QTLIL ++T R DW+KE  KA+  +  W DKK+ L
Sbjct:  460 IWTGMI-GGTLMQTLILLYVTYRTDWDKEVEKARKRLDLWDDKKEPL 505

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT4G21910.2 (| Symbols: | MATE efflux family protein | chr4:11625742-11629253 REVERSE LENGTH=509)

HSP 1 Score: 56.6102 bits (135), Expect = 3.662e-9
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKKQQL 141
            IW GMI GGT  QTLIL ++T R DW+KE  KA+  +  W DKK+ L
Sbjct:  462 IWTGMI-GGTLMQTLILLYVTYRTDWDKEVEKARKRLDLWDDKKEPL 507

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT4G21903.1 (| Symbols: | MATE efflux family protein | chr4:11621169-11623738 REVERSE LENGTH=504)

HSP 1 Score: 52.373 bits (124), Expect = 6.907e-8
Identity = 23/44 (52.27%), Postives = 30/44 (68.18%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKK 132
            IW GMI GGT  QTLIL ++T + DW+KE  KA+  +  W DK+
Sbjct:  458 IWTGMI-GGTLMQTLILLYVTYQADWDKEVEKARKRLDMWDDKE 500

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT1G61890.1 (| Symbols: | MATE efflux family protein | chr1:22868103-22871163 REVERSE LENGTH=501)

HSP 1 Score: 51.6026 bits (122), Expect = 1.178e-7
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSDKKQQL 141
            IW GMI GGT  QT+IL  +TLR DW+KE  KA   + +W + ++ L
Sbjct:  453 IWTGMI-GGTLMQTIILVIVTLRTDWDKEVEKASSRLDQWEESREPL 498

[Close]

BLAST of GH719505 vs. TAIR peptide
Match: AT1G11670.1 (| Symbols: | MATE efflux family protein | chr1:3928520-3931482 REVERSE LENGTH=503)

HSP 1 Score: 48.9062 bits (115), Expect = 7.636e-7
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 1

Query:    1 IWAGMIFGGTATQTLILCWITLRCDWEKEAAKAKLHVSKWSD 126
            IW GMI GGT  QT+IL  +T R DW+KE  KA   + +W D
Sbjct:  456 IWTGMI-GGTLMQTIILVIVTFRTDWDKEVEKASRRLDQWED 496

The following BLAST results are available for this feature:

BLAST of GH719505 vs. TrEMBL
BLAST of GH719505 vs. TAIR peptide

BLAST of GH719505 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T6V3_RICCO	1.500e-12	75.00	Multidrug resistance pump, putative OS=Ricinus com...	[more]
D7UE71_VITVI	2.558e-12	78.57	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5ACN2_VITVI	2.558e-12	78.57	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9HFS0_POPTR	7.443e-12	78.57	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MN36_ARALY	9.721e-12	78.05	Mate efflux family protein OS=Arabidopsis lyrata s...	[more]
D7MTX6_ARALY	1.658e-11	73.81	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q4ABZ9_BRACM	2.166e-11	75.61	117M18_21 OS=Brassica campestris GN=117M18_21 PE=4...	[more]
Q9FNC1_ARATH	3.694e-11	75.61	Emb\|CAB89401.1 OS=Arabidopsis thaliana GN=At5g4405...	[more]
Q4ABZ7_BRACM	4.824e-11	75.61	117M18_23 OS=Brassica campestris GN=117M18_23 PE=4...	[more]
Q9FKQ1_ARATH	8.229e-11	69.05	AT5g65380/MNA5_11 OS=Arabidopsis thaliana GN=At5g6...	[more]

BLAST of GH719505 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G44050.1	1.437e-13	75.61	\| Symbols: \| MATE efflux family protein \| chr5:17...	[more]
AT5G65380.1	3.201e-13	69.05	\| Symbols: \| MATE efflux family protein \| chr5:26...	[more]
AT5G10420.1	3.913e-11	67.50	\| Symbols: \| MATE efflux family protein \| chr5:32...	[more]
AT3G21690.1	4.326e-10	55.32	\| Symbols: \| MATE efflux family protein \| chr3:76...	[more]
AT4G21910.3	3.662e-9	55.32	\| Symbols: \| MATE efflux family protein \| chr4:11...	[more]
AT4G21910.1	3.662e-9	55.32	\| Symbols: \| MATE efflux family protein \| chr4:11...	[more]
AT4G21910.2	3.662e-9	55.32	\| Symbols: \| MATE efflux family protein \| chr4:11...	[more]
AT4G21903.1	6.907e-8	52.27	\| Symbols: \| MATE efflux family protein \| chr4:11...	[more]
AT1G61890.1	1.178e-7	48.94	\| Symbols: \| MATE efflux family protein \| chr1:22...	[more]
AT1G11670.1	7.636e-7	52.38	\| Symbols: \| MATE efflux family protein \| chr1:39...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 18..29 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719505 ID=GH719505; Name=GH719505; organism=Pisum sativum; type=EST; length=257bp
ATTTGGGCAGGAATGATTTTTGGAGGAACAGCAACTCAGACATTAATATT
GTGTTGGATTACACTTAGGTGTGATTGGGAGAAAGAGGCAGCGAAAGCAA
AATTGCATGTTTCAAAGTGGTCAGATAAAAAACAACAACTCGGGTAGTGT
TTGTTTTGTTTAAGATATTTAGAAGAAGTTGAAAAATGTAATTGTTCATT
ACTTCTTTGAAGGGGTTGTCTCTTTGTAACTAAATTGGATAGGAATTAGT
ATTATTT

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