GH719516

EST Overview


Name	GH719516
Unique Name	GH719516
Type	EST
Organism	Pisum sativum (pea)
Sequence length	188

Libraries


Library Name	Type
LIBEST_024268 TI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q2ENF9_ASTMO (Isoflavone 2'-hydroxylase (Fragment) OS=Astragalus mongholicus PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.224e-23
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 1

Query:    4 AGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            AGTDSSAVTLEWALSCVL  PEVL+K R EL+TH+GQDRLL+ESDLPK+PYLKNI+YETLR
Sbjct:    7 AGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLR 67

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.177e-21
Identity = 49/62 (79.03%), Postives = 57/62 (91.94%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW++S +L +PEVL+K R EL+THVGQDRL+DESDLPKL YLKN+IYETLR
Sbjct:  301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLR 362

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.177e-21
Identity = 48/62 (77.42%), Postives = 56/62 (90.32%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW +S +L YPEVL+K R E++THVGQDRL+DESDLPKL YL+N+IYETLR
Sbjct:  301 LAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLR 362

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.724e-20
Identity = 48/62 (77.42%), Postives = 55/62 (88.71%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW++  VL YPEVLEK + EL+THVGQDRL+DESD+PKL YLKN+I ETLR
Sbjct:  302 LAGTDSSAVTLEWSMCNVLNYPEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLR 363

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 7.476e-20
Identity = 46/62 (74.19%), Postives = 56/62 (90.32%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW++S +L +PE+L+K + EL+ HVGQDRL+DESDLPKL YLKN+IYETLR
Sbjct:  301 LAGTDSSAVTLEWSMSNLLNHPEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLR 362

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.275e-19
Identity = 47/62 (75.81%), Postives = 56/62 (90.32%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW++S +L +PEVL+K + EL+THVGQDRL+DESDLPKL YLKN+I ETLR
Sbjct:  301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLR 362

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.036e-17
Identity = 45/60 (75.00%), Postives = 52/60 (86.67%), Query Frame = 1

Query:    7 GTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            GTDSS  TLEW+LS +L +PEVL+KAR EL+T VGQDRLL+ESDLPKLPYL+ II ETLR
Sbjct:  307 GTDSSTGTLEWSLSNLLNHPEVLKKARDELDTQVGQDRLLNESDLPKLPYLRKIILETLR 366

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.923e-17
Identity = 38/62 (61.29%), Postives = 55/62 (88.71%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+S+VT+EWA+S +L +PE+++KA+ EL+TH+G DR +DE D+ KLPYL++I+YETLR
Sbjct:  301 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLR 362

[Close]

BLAST of GH719516 vs. TrEMBL
Match: D7KU98_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_475761 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.923e-17
Identity = 42/62 (67.74%), Postives = 54/62 (87.10%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEWALS +L +PEVL+KAR E++  +G +RLL+ESD+P LPYL+NI+ ETLR
Sbjct:  183 LAGTDTSAVTLEWALSSLLNHPEVLKKARDEIDNKIGLNRLLEESDVPNLPYLQNIVSETLR 244

[Close]

BLAST of GH719516 vs. TrEMBL
Match: Q9C714_ARATH (Cytochrome P450, putative OS=Arabidopsis thaliana GN=F28G11.4 PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.736e-17
Identity = 42/62 (67.74%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEWALS +L  PEVL+K R E++  +G DRLL+ESD+P LPYL+NI+ ETLR
Sbjct:  281 LAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLR 342

[Close]

BLAST of GH719516 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 6.302e-21
Identity = 47/62 (75.81%), Postives = 56/62 (90.32%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTDSSAVTLEW++S +L +PEVL+K + EL+THVGQDRL+DESDLPKL YLKN+I ETLR
Sbjct:  301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLR 362

[Close]

BLAST of GH719516 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.210e-17
Identity = 39/62 (62.90%), Postives = 53/62 (85.48%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGT++SAVTLEWALS +L +P+V+ KAR E++  VG DRL++E+DL +LPYLKNI+ ETLR
Sbjct:  305 IAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 366

[Close]

BLAST of GH719516 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 83.9593 bits (206), Expect = 2.740e-16
Identity = 38/62 (61.29%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGT++SAVTLEWA++ +L  PEVLEKAR E++  +G+DRL+DESD+  LPYL+N++ ET R
Sbjct:  299 LAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFR 360

[Close]

BLAST of GH719516 vs. SwissProt
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.964e-14
Identity = 35/62 (56.45%), Postives = 51/62 (82.26%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGTD+SAVT+EWA++ ++  P VLEKARQE++  VG+ R+++ESD+  LPYL+ I+ ETLR
Sbjct:  306 IAGTDTSAVTMEWAMAELINNPGVLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLR 367

[Close]

BLAST of GH719516 vs. SwissProt
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.374e-14
Identity = 34/62 (54.84%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGTD+SAV++EWA++ ++  P+VLEKARQE++  VG+ R+++ESD+  LPYL+ I+ ETLR
Sbjct:  305 VAGTDTSAVSIEWAMAELINNPDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLR 366

[Close]

BLAST of GH719516 vs. SwissProt
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.835e-13
Identity = 34/62 (54.84%), Postives = 49/62 (79.03%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGTD+SA T+EWA++ ++  P VLEKARQE++  VG  R+++ESD+  LPYL+ I+ ETLR
Sbjct:  298 VAGTDTSAATMEWAMAELINNPCVLEKARQEIDAVVGNSRIIEESDIVNLPYLQAIVRETLR 359

[Close]

BLAST of GH719516 vs. SwissProt
Match: C93C1_SOYBN (2-hydroxyisoflavanone synthase OS=Glycine max GN=IFS2 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.407e-12
Identity = 34/61 (55.74%), Postives = 47/61 (77.05%), Query Frame = 1

Query:    4 AGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            AGTDS+AV  EWAL+ ++  P+VLEKAR+E+ + VG+DRL+DE D   LPY++ I+ ET R
Sbjct:  304 AGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEVDTQNLPYIRAIVKETFR 364

[Close]

BLAST of GH719516 vs. SwissProt
Match: C93B1_GLYEC (Licodione synthase OS=Glycyrrhiza echinata GN=CYP93B1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 2.400e-12
Identity = 32/61 (52.46%), Postives = 47/61 (77.05%), Query Frame = 1

Query:    4 AGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            AGTD++A++ EWAL  ++  P VL+K R+E++  VG+DRL++ESD P LPYL+ I+ ET R
Sbjct:  304 AGTDTTAISTEWALVELVKKPSVLQKVREEIDNVVGKDRLVEESDCPNLPYLQAILKETFR 364

[Close]

BLAST of GH719516 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.191e-11
Identity = 32/62 (51.61%), Postives = 47/62 (75.81%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            L G+D++A TL WA+  +L +P VLEK ++EL T++G++R ++ESD+ KL YL  II ETLR
Sbjct:  339 LGGSDTTAGTLTWAMCLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLR 400

[Close]

BLAST of GH719516 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.556e-11
Identity = 33/62 (53.23%), Postives = 45/62 (72.58%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            L GTDS+AVTL WALS +L  P  L KA++E++  +G+D  + ESD+ KL YL+ I+ ETLR
Sbjct:  322 LGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLR 383

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327)

HSP 1 Score: 90.1225 bits (222), Expect = 2.948e-19
Identity = 42/62 (67.74%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEWALS +L  PEVL+K R E++  +G DRLL+ESD+P LPYL+NI+ ETLR
Sbjct:  127 LAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLR 188

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 90.1225 bits (222), Expect = 2.948e-19
Identity = 42/62 (67.74%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEWALS +L  PEVL+K R E++  +G DRLL+ESD+P LPYL+NI+ ETLR
Sbjct:  186 LAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLR 247

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 88.9669 bits (219), Expect = 6.568e-19
Identity = 42/62 (67.74%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEWALS VL +P+VL KAR E++  +G DRL+DESD+  LPYL+NI+ ETLR
Sbjct:  298 LAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLR 359

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 85.8853 bits (211), Expect = 5.560e-18
Identity = 39/62 (62.90%), Postives = 53/62 (85.48%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGT++SAVTLEWALS +L +P+V+ KAR E++  VG DRL++E+DL +LPYLKNI+ ETLR
Sbjct:  305 IAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 366

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 85.8853 bits (211), Expect = 5.560e-18
Identity = 40/60 (66.67%), Postives = 50/60 (83.33%), Query Frame = 1

Query:    7 GTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            GTD++AVTLEWALS +L  PEVL KAR E++  +G DRLL+ESD+P LPYL+NI+ ETLR
Sbjct:  301 GTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLR 360

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37400.1 (| Symbols: CYP81F3 | cytochrome P450, family 81, subfamily F, polypeptide 3 | chr4:17584096-17586197 FORWARD LENGTH=501)

HSP 1 Score: 84.3445 bits (207), Expect = 1.618e-17
Identity = 37/62 (59.68%), Postives = 51/62 (82.26%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGTD+SAVTLEWA+S +L +PE LEKA+ E++  +GQ+RL+DE D+  LPYL+NI+ ET R
Sbjct:  294 IAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPYLQNIVSETFR 355

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37430.1 (| Symbols: CYP91A2, CYP81F1 | cytochrome P450, family 91, subfamily A, polypeptide 2 | chr4:17597242-17598829 FORWARD LENGTH=500)

HSP 1 Score: 83.9593 bits (206), Expect = 2.113e-17
Identity = 38/62 (61.29%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGT++SAVTLEWA++ +L  PEVLEKAR E++  +G+DRL+DESD+  LPYL+N++ ET R
Sbjct:  299 LAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFR 360

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 83.1889 bits (204), Expect = 3.604e-17
Identity = 38/62 (61.29%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+S+VTLEWA+S +L +PE+LEKAR E++  +G DRL++ESD+  L YL+NI+ ETLR
Sbjct:  299 LAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLR 360

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 82.8037 bits (203), Expect = 4.707e-17
Identity = 38/62 (61.29%), Postives = 51/62 (82.26%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            LAGTD+SAVTLEW LS +L +P++L KAR E++  VG +RL++ESDL  LPYL+NI+ E+LR
Sbjct:  299 LAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLR 360

[Close]

BLAST of GH719516 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 81.2629 bits (199), Expect = 1.370e-16
Identity = 37/62 (59.68%), Postives = 52/62 (83.87%), Query Frame = 1

Query:    1 LAGTDSSAVTLEWALSCVLTYPEVLEKARQELETHVGQDRLLDESDLPKLPYLKNIIYETLR 186
            +AGTD+S+VTLEWA+S +L +PE+L+KAR E++  VG DRL+DESD+  L YL++I+ ETLR
Sbjct:  296 IAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLR 357

The following BLAST results are available for this feature:

BLAST of GH719516 vs. TrEMBL
BLAST of GH719516 vs. SwissProt
BLAST of GH719516 vs. TAIR peptide

BLAST of GH719516 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2ENF9_ASTMO	7.224e-23	83.61	Isoflavone 2'-hydroxylase (Fragment) OS=Astragalus...	[more]
Q9XFX0_CICAR	5.177e-21	79.03	Cytochrome P450 monooxygenase OS=Cicer arietinum G...	[more]
Q6WNR0_MEDTR	5.177e-21	77.42	Isoflavone 2'-hydroxylase OS=Medicago truncatula P...	[more]
Q9MBE4_LOTJA	5.724e-20	77.42	Cytochrome P450 OS=Lotus japonicus PE=2 SV=1	[more]
Q9ZRW6_CICAR	7.476e-20	74.19	Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2...	[more]
Q9ZWF2_GLYEC	1.275e-19	75.81	Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-...	[more]
Q2LAK9_SOYBN	2.036e-17	75.00	Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ...	[more]
Q6WNQ8_MEDTR	5.923e-17	61.29	CYP81E8 OS=Medicago truncatula PE=2 SV=1	[more]
D7KU98_ARALY	5.923e-17	67.74	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9C714_ARATH	7.736e-17	67.74	Cytochrome P450, putative OS=Arabidopsis thaliana ...	[more]

BLAST of GH719516 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
C81E1_GLYEC	6.302e-21	75.81	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ...	[more]
C81D1_ARATH	7.210e-17	62.90	Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY...	[more]
C81F1_ARATH	2.740e-16	61.29	Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY...	[more]
C93A3_SOYBN	1.964e-14	56.45	Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=...	[more]
C93A1_SOYBN	4.374e-14	54.84	Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=...	[more]
C93A2_SOYBN	2.835e-13	54.84	Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=...	[more]
C93C1_SOYBN	1.407e-12	55.74	2-hydroxyisoflavanone synthase OS=Glycine max GN=I...	[more]
C93B1_GLYEC	2.400e-12	52.46	Licodione synthase OS=Glycyrrhiza echinata GN=CYP9...	[more]
C82A1_PEA	1.191e-11	51.61	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G...	[more]
C82A3_SOYBN	1.556e-11	53.23	Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=...	[more]

BLAST of GH719516 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G66540.2	2.948e-19	67.74	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT1G66540.1	2.948e-19	67.74	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT4G37370.1	6.568e-19	67.74	\| Symbols: CYP81D8 \| cytochrome P450, family 81, s...	[more]
AT5G36220.1	5.560e-18	62.90	\| Symbols: CYP81D1, CYP91A1 \| cytochrome p450 81d1...	[more]
AT4G37360.1	5.560e-18	66.67	\| Symbols: CYP81D2 \| cytochrome P450, family 81, s...	[more]
AT4G37400.1	1.618e-17	59.68	\| Symbols: CYP81F3 \| cytochrome P450, family 81, s...	[more]
AT4G37430.1	2.113e-17	61.29	\| Symbols: CYP91A2, CYP81F1 \| cytochrome P450, fam...	[more]
AT4G37320.1	3.604e-17	61.29	\| Symbols: CYP81D5 \| cytochrome P450, family 81, s...	[more]
AT4G37340.1	4.707e-17	61.29	\| Symbols: CYP81D3 \| cytochrome P450, family 81, s...	[more]
AT4G37330.1	1.370e-16	59.68	\| Symbols: CYP81D4 \| cytochrome P450, family 81, s...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 1..62 score: 3.3
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 1..62 score: 8.3
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..62 score: 6.8
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 1..62 score: 5.1
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 11..37 score: 4.2e-07coord: 54..62 score: 4.2
None	No IPR available	PANTHER	PTHR19383:SF42	CYTOCHROME P450 SUBFAMILY 81D	coord: 1..62 score: 8.3

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719516 ID=GH719516; Name=GH719516; organism=Pisum sativum; type=EST; length=188bp
CTTGCTGGAACAGACTCATCAGCTGTAACCTTAGAGTGGGCATTATCTTG
TGTATTGACCTATCCAGAAGTGCTAGAGAAGGCAAGACAAGAACTGGAAA
CTCACGTAGGTCAAGACCGTTTATTGGATGAATCCGACCTTCCAAAACTC
CCTTACCTTAAAAACATAATCTACGAGACACTTAGGTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I

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