GH719897

EST Overview


Name	GH719897
Unique Name	GH719897
Type	EST
Organism	Pisum sativum (pea)
Sequence length	578

Libraries


Library Name	Type
LIBEST_024269 TNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH719897 vs. TrEMBL
Match: C6T7D9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 355.14 bits (910), Expect = 1.944e-96
Identity = 174/191 (91.10%), Postives = 185/191 (96.86%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            N+LKGKVKLGHVDCDA+KSLMSRF VQGFPTILVFGADKD+P+PYEGARTA AIESFALEQLETNVAPPEVTEL+S D+LEEKCGSAAICFVAFLPDILDSKAEGRN YLQQLL+VAE+FKRSPYSY WVAAG QPDLE+NVGVGGYGYPALVALN+KKAVYAPLKSAFELDQIIEFVKEAGRGG+GNLPL
Sbjct:  207 NSLKGKVKLGHVDCDAEKSLMSRFKVQGFPTILVFGADKDSPIPYEGARTALAIESFALEQLETNVAPPEVTELHSPDVLEEKCGSAAICFVAFLPDILDSKAEGRNIYLQQLLSVAEKFKRSPYSYVWVAAGNQPDLEKNVGVGGYGYPALVALNLKKAVYAPLKSAFELDQIIEFVKEAGRGGKGNLPL 397

[Close]

BLAST of GH719897 vs. TrEMBL
Match: A9CPA7_SOYBN (Protein disulfide isomerase family OS=Glycine max GN=GmPDIM PE=3 SV=1)

HSP 1 Score: 355.14 bits (910), Expect = 1.944e-96
Identity = 174/191 (91.10%), Postives = 185/191 (96.86%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            N+LKGKVKLGHVDCDA+KSLMSRF VQGFPTILVFGADKD+P+PYEGARTA AIESFALEQLETNVAPPEVTEL+S D+LEEKCGSAAICFVAFLPDILDSKAEGRN YLQQLL+VAE+FKRSPYSY WVAAG QPDLE+NVGVGGYGYPALVALN+KKAVYAPLKSAFELDQIIEFVKEAGRGG+GNLPL
Sbjct:  207 NSLKGKVKLGHVDCDAEKSLMSRFKVQGFPTILVFGADKDSPIPYEGARTALAIESFALEQLETNVAPPEVTELHSPDVLEEKCGSAAICFVAFLPDILDSKAEGRNIYLQQLLSVAEKFKRSPYSYVWVAAGNQPDLEKNVGVGGYGYPALVALNLKKAVYAPLKSAFELDQIIEFVKEAGRGGKGNLPL 397

[Close]

BLAST of GH719897 vs. TrEMBL
Match: B9IAL5_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_246818 PE=3 SV=1)

HSP 1 Score: 346.665 bits (888), Expect = 6.914e-94
Identity = 167/191 (87.43%), Postives = 180/191 (94.24%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            NNL+GKVKLGHVDCD++KSLMSRFNVQGFPTILVFGADKDTP+PYEGARTASAIESFALEQLE+NVAPPEVTEL   D++EEKCGSAAICFVAFLPDILDSKAEGRNKYL+QLL+VAE+FKRSPYSY W AAGKQPDLE  VGVGGYGYPALVALN KK  YAPLKSAFEL+ I+EFVKEAGRGG+GNLPL
Sbjct:  180 NNLQGKVKLGHVDCDSEKSLMSRFNVQGFPTILVFGADKDTPIPYEGARTASAIESFALEQLESNVAPPEVTELTGPDVMEEKCGSAAICFVAFLPDILDSKAEGRNKYLEQLLSVAEKFKRSPYSYVWAAAGKQPDLENRVGVGGYGYPALVALNAKKGAYAPLKSAFELEHIVEFVKEAGRGGKGNLPL 370

[Close]

BLAST of GH719897 vs. TrEMBL
Match: D7TEF8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_59.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030525001 PE=4 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 2.905e-92
Identity = 166/191 (86.91%), Postives = 178/191 (93.19%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            NNLKGKVKLGHVDCD+DKSLMSRF+VQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTEL   D++EEKCGSAAICFVAFLPDILDSKAEGRNKYL+ +L++AE+FKRSPYSY W AAGKQ DLE+ VGVGGYGYPALVALN+KK  YAPLKSAFELDQII FVKEAG GG+G LPL
Sbjct:  206 NNLKGKVKLGHVDCDSDKSLMSRFSVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELTGPDVMEEKCGSAAICFVAFLPDILDSKAEGRNKYLEMMLSIAEKFKRSPYSYVWAAAGKQADLEKQVGVGGYGYPALVALNVKKGAYAPLKSAFELDQIIGFVKEAGHGGKGILPL 396

[Close]

BLAST of GH719897 vs. TrEMBL
Match: C0PPU0_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.182e-85
Identity = 150/191 (78.53%), Postives = 176/191 (92.15%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            NNLKGKV +G V+CD++KSLMSRFNVQGFPTILVFG+DK++PVPYEGARTASAIESFALEQLETNV+PPEV EL S D+LE KC S+AICFV+FLPDILD++AEGRN YL+QL++VAE+FKRSPYSY W AAGKQP LE+ VGVGGYGYPA +ALNIKK VYAPL+SAFEL+ +++FVK+AGRGG+GNLPL
Sbjct:  213 NNLKGKVNMGQVNCDSEKSLMSRFNVQGFPTILVFGSDKESPVPYEGARTASAIESFALEQLETNVSPPEVVELTSQDVLETKCASSAICFVSFLPDILDTRAEGRNSYLEQLMSVAEKFKRSPYSYVWAAAGKQPALERAVGVGGYGYPAFIALNIKKGVYAPLRSAFELEHLVDFVKDAGRGGKGNLPL 403

HSP 2 Score: 58.5362 bits (140), Expect = 3.761e-7
Identity = 31/64 (48.44%), Postives = 40/64 (62.50%), Query Frame = -2

Query:  380 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNV 571
            LKG V +  +D DA KSL   + +QGFPTI VF   K  P+ Y+GAR    I  FA++QL+T V
Sbjct:   86 LKGFVTVAALDADAHKSLAQEYGIQGFPTIKVFTPGK-PPIAYQGARDPKPIAEFAIQQLKTIV 148

[Close]

BLAST of GH719897 vs. TrEMBL
Match: Q67UF5_ORYSJ (Os09g0451500 protein OS=Oryza sativa subsp. japonica GN=OJ1163_C07.26-2 PE=3 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 2.912e-84
Identity = 149/190 (78.42%), Postives = 172/190 (90.53%), Query Frame = -2

Query:    5 NLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 574
            NLKG+VKLGHVDCDA+KSLMS++ V+GFPTILVFGADK++P PY+GAR ASAIESFALEQLE N APPEV+EL   D +EEKC SAAICFV+FLPDILDSKAEGRNKYL+ LL+VAE+FK+SPYS+ W AAGKQ DLE+ VGVGGYGYPA+VALN+KK  YAPL+SAF+LD+I EFVKEAGRGG+GNLPL
Sbjct:  211 NLKGQVKLGHVDCDAEKSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANAAPPEVSELTGPDAMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKKSPYSFVWTAAGKQADLEKQVGVGGYGYPAMVALNVKKGAYAPLRSAFQLDEITEFVKEAGRGGKGNLPL 400

[Close]

BLAST of GH719897 vs. TrEMBL
Match: Q5EUD5_MAIZE (Protein disulfide isomerase OS=Zea mays GN=PDIL2-3 PE=2 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 2.725e-82
Identity = 146/190 (76.84%), Postives = 170/190 (89.47%), Query Frame = -2

Query:    5 NLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 574
            NLKG+VKLGHVDCDA+KSLMS++ V+GFPTILVFGADK++P PY+GAR ASAIESFALEQLE N  P EV+EL   D++EEKC SAAICFV+FLPDILDSKAEGRNKYL+ LL+VAE+FK+SPYS+ W AAGKQ +LE  VGVGGYGYPA+VALN+KK  YAPL+SAF+ D+IIEFVKEAGRGG+GNLPL
Sbjct:  209 NLKGQVKLGHVDCDAEKSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANSGPAEVSELTGPDVMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKKSPYSFVWTAAGKQANLENQVGVGGYGYPAMVALNVKKGAYAPLRSAFQRDEIIEFVKEAGRGGKGNLPL 398

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BLAST of GH719897 vs. TrEMBL
Match: B8BCJ0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_31602 PE=3 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 1.036e-81
Identity = 150/204 (73.53%), Postives = 172/204 (84.31%), Query Frame = -2

Query:    5 NLKGKVKLGHVDCDADK--------------SLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 574
            NLKG+VKLGHVDCDA+K              SLMS++ V+GFPTILVFGADK++P PY+GAR ASAIESFALEQLE N APPEV+EL   D +EEKC SAAICFV+FLPDILDSKAEGRNKYL+ LL+VAE+FKRSPYS+ W AAGKQ DLE+ VGVGGYGYPA+VALN+KK  YAPL+SAF+LD+I EFVKEAGRGG+GNLPL
Sbjct:  211 NLKGQVKLGHVDCDAEKNTNSSVWSVDKSYRSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANAAPPEVSELTGPDAMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKRSPYSFVWTAAGKQADLEKQVGVGGYGYPAMVALNVKKGAYAPLRSAFQLDEITEFVKEAGRGGKGNLPL 414

[Close]

BLAST of GH719897 vs. TrEMBL
Match: Q9MAU6_ARATH (Disulfide isomerase-related protein, putative OS=Arabidopsis thaliana GN=At1g04980 PE=2 SV=1)

HSP 1 Score: 305.834 bits (782), Expect = 1.353e-81
Identity = 143/191 (74.87%), Postives = 169/191 (88.48%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            NNLKGKVKLGHV+CDA++S+ SRF VQGFPTILVFG+DK +PVPYEGAR+ASAIESFALEQLE+N  P EVTEL   D++E+KCGSAAICFV+FLPDILDSKAEGRNKYL+ LL+VA++FK+ PY + WVAAGKQPDLE+ VGVGGYGYPA+VALN KK  YAPLKS FE+  + +FVKEA +GG+GNLP+
Sbjct:  208 NNLKGKVKLGHVNCDAEQSIKSRFKVQGFPTILVFGSDKSSPVPYEGARSASAIESFALEQLESNAGPAEVTELTGPDVMEDKCGSAAICFVSFLPDILDSKAEGRNKYLEMLLSVADKFKKDPYGFVWVAAGKQPDLEKRVGVGGYGYPAMVALNAKKGAYAPLKSGFEVKHLKDFVKEAAKGGKGNLPI 398

[Close]

BLAST of GH719897 vs. TrEMBL
Match: D7KE98_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_470488 PE=4 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 2.551e-80
Identity = 141/191 (73.82%), Postives = 167/191 (87.43%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            N L+GKVKLGHV+CDA++S+ SRF VQGFPTILVFGADK +PVPYEGAR+ASAIESFALEQLE+N  P EVTEL   D++EEKCG AAICFV+FLPDILDSKAEGRNKYL+ LL+VA++FK+ PY + WVAAGKQPDLE+ VGVGGYGYPA+VALN KK  YAPLKS FE+  + +F+KEA +GG+GNLP+
Sbjct:  207 NKLQGKVKLGHVNCDAEQSIKSRFKVQGFPTILVFGADKSSPVPYEGARSASAIESFALEQLESNAGPVEVTELTGPDVMEEKCGPAAICFVSFLPDILDSKAEGRNKYLEMLLSVADKFKKDPYGFVWVAAGKQPDLEKRVGVGGYGYPAMVALNAKKGAYAPLKSGFEVKHLKDFIKEAAKGGKGNLPI 397

HSP 2 Score: 58.151 bits (139), Expect = 4.912e-7
Identity = 29/64 (45.31%), Postives = 41/64 (64.06%), Query Frame = -2

Query:  386 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLET 577
            N LKG   +  +D DA KS+   + V+GFPTI VF   K  P+ Y+GAR A +I  FA++Q++T
Sbjct:   77 NTLKGIATVAAIDADAHKSVSQDYGVRGFPTIKVFVPGK-PPIDYQGARDAKSISQFAIKQIKT 139

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_RAT (Protein disulfide-isomerase A6 OS=Rattus norvegicus GN=Pdia6 PE=1 SV=2)

HSP 1 Score: 160.999 bits (406), Expect = 3.870e-39
Identity = 81/189 (42.86%), Postives = 118/189 (62.43%), Query Frame = -2

Query:    2 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPLG 568
            KGKVKL  VD   ++ L SR+ ++GFPTI +F    ++PV Y+G RT S I S AL+    N  PPE+ E+ + DI ++ C    +C VA LP ILD+ A GRN YL+ LL +A+++K+  + + W  AG Q +LE  +G+GG+GYPA+ A+N +K  +A LK +F    I EF++E   G     P+G
Sbjct:  212 KGKVKLAAVDATVNQVLASRYGIKGFPTIKIFQKG-ESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGATGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVG 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.214e-8
Identity = 30/60 (50.00%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  392 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQL 571
            LK  VK+G V+ D  +SL  ++ VQGFPTI +FGA+K+ P  Y+G RT  AI   AL  L
Sbjct:   72 LKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSAL 131

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_PONAB (Protein disulfide-isomerase A6 OS=Pongo abelii GN=PDIA6 PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 3.870e-39
Identity = 81/189 (42.86%), Postives = 117/189 (61.90%), Query Frame = -2

Query:    2 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPLG 568
            KGKVKL  VD   ++ L SR+ ++GFPTI +F    ++PV Y+G RT S I S AL+    N  PPE+ E+ S DI +  C    +C V+ LP ILD+ A GRN YL+ LL +A+++K+  + + W  AG Q +LE  +G+GG+GYPA+ A+N +K  +A LK +F    I EF++E   G     P+G
Sbjct:  212 KGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKG-ESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIISEDIAKRTCEEHQLCVVSVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVG 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.214e-8
Identity = 30/60 (50.00%), Postives = 38/60 (63.33%), Query Frame = -2

Query:  392 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQL 571
            LK  VK+G VD D   SL  ++ VQGFPTI +FG++K+ P  Y+G RT  AI   AL  L
Sbjct:   72 LKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSAL 131

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_MOUSE (Protein disulfide-isomerase A6 OS=Mus musculus GN=Pdia6 PE=1 SV=3)

HSP 1 Score: 160.999 bits (406), Expect = 3.870e-39
Identity = 81/189 (42.86%), Postives = 118/189 (62.43%), Query Frame = -2

Query:    2 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPLG 568
            KGKVKL  VD   ++ L SR+ ++GFPTI +F    ++PV Y+G RT S I S AL+    N  PPE+ E+ + DI ++ C    +C VA LP ILD+ A GRN YL+ LL +A+++K+  + + W  AG Q +LE  +G+GG+GYPA+ A+N +K  +A LK +F    I EF++E   G     P+G
Sbjct:  212 KGKVKLAAVDATVNQVLASRYGIKGFPTIKIFQKG-ESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQYELENALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVG 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.214e-8
Identity = 30/60 (50.00%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  392 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQL 571
            LK  VK+G V+ D  +SL  ++ VQGFPTI +FGA+K+ P  Y+G RT  AI   AL  L
Sbjct:   72 LKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSAL 131

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_HUMAN (Protein disulfide-isomerase A6 OS=Homo sapiens GN=PDIA6 PE=1 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 3.870e-39
Identity = 81/189 (42.86%), Postives = 117/189 (61.90%), Query Frame = -2

Query:    2 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPLG 568
            KGKVKL  VD   ++ L SR+ ++GFPTI +F    ++PV Y+G RT S I S AL+    N  PPE+ E+ + DI +  C    +C VA LP ILD+ A GRN YL+ LL +A+++K+  + + W  AG Q +LE  +G+GG+GYPA+ A+N +K  +A LK +F    I EF++E   G     P+G
Sbjct:  212 KGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKG-ESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVG 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.214e-8
Identity = 30/60 (50.00%), Postives = 38/60 (63.33%), Query Frame = -2

Query:  392 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQL 571
            LK  VK+G VD D   SL  ++ VQGFPTI +FG++K+ P  Y+G RT  AI   AL  L
Sbjct:   72 LKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSAL 131

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_MESAU (Protein disulfide-isomerase A6 OS=Mesocricetus auratus GN=PDIA6 PE=1 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 3.066e-36
Identity = 77/189 (40.74%), Postives = 115/189 (60.85%), Query Frame = -2

Query:    2 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPLG 568
            KGKVKL  VD   ++ L +R+ ++GFPTI +F    + PV Y+G RT S I S AL+    N  PPE+ E+ + D+ ++ C    +C VA LP ILD+ A  RN YL+ LL +A+++K+  + + W  AG Q +LE  +G+GG+GYPA+  +N +K  +A LK +F    I EF++E   G     P+G
Sbjct:  212 KGKVKLAAVDATVNQVLANRYGIRGFPTIKIFQKG-EAPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDVAKKMCEEHQLCVVAVLPHILDTGA-ARNSYLEILLKLADKYKKKMWGWLWTEAGAQSELENALGIGGFGYPAMARINARKMKFALLKGSFSEQGINEFLRELSFGRASTAPVG 398

HSP 2 Score: 61.6178 bits (148), Expect = 3.195e-9
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  392 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQL 571
            LK  VK+G VD D  +SL  ++ VQGFPTI +FGA+K+ P  Y+G RT  AI   AL  L
Sbjct:   72 LKDVVKVGAVDADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSAL 131

[Close]

BLAST of GH719897 vs. SwissProt
Match: PDIA6_CAEEL (Probable protein disulfide-isomerase A6 OS=Caenorhabditis elegans GN=tag-320 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 6.394e-34
Identity = 74/186 (39.78%), Postives = 112/186 (60.22%), Query Frame = -2

Query:   26 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVF--GADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRG 577
            + LKGKV+LG +D      + ++F ++GFPTI  F  G+D      Y+G R +S I ++A  + + N+  PEV E  +  ++E+ C    +C  AFLP ILD ++E RN YL  L   +E+FK++ + + WV    QP LE++  VGG+GYPA+ ALN +K  YA LK +F  D I EF+++   G
Sbjct:  209 SELKGKVRLGALDATVHTVVANKFAIRGFPTIKYFAPGSDVSDAQDYDGGRQSSDIVAWASARAQENMPAPEVFEGINQQVVEDACKEKQLCIFAFLPHILDCQSECRNNYLAMLKEQSEKFKKNLWGWIWVEGAAQPALEESFEVGGFGYPAMTALNFRKNKYAVLKGSFGKDGIHEFLRDLSYG 394

HSP 2 Score: 60.077 bits (144), Expect = 9.297e-9
Identity = 30/65 (46.15%), Postives = 39/65 (60.00%), Query Frame = -2

Query:  377 LKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVA 571
            LKG  K+G VD    +S+   +NVQGFPT+ +FGADK  P  Y G RTA AI    L + +  V+
Sbjct:   71 LKGVAKVGAVDMTQHQSVGGPYNVQGFPTLKIFGADKKKPTDYNGQRTAQAIADSVLAEAKKAVS 135

[Close]

BLAST of GH719897 vs. SwissProt
Match: Y5025_DICDI (Putative protein disulfide-isomerase DDB_G0275025 OS=Dictyostelium discoideum GN=DDB_G0275025 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.890e-10
Identity = 31/70 (44.29%), Postives = 45/70 (64.29%), Query Frame = -2

Query:  383 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGAD-----KDTPVPYEGARTASAIESFALEQLETN 577
            NNLKG VK+G ++CD +K L  ++ +QGFPT+  F  +     K  P  Y+GAR+AS I  F+L +L +N
Sbjct:   72 NNLKGLVKIGAINCDEEKELCGQYQIQGFPTLKFFSTNPKTGKKGQPEDYQGARSASEIAKFSLAKLPSN 141

[Close]

BLAST of GH719897 vs. SwissProt
Match: ERP38_NEUCR (Protein disulfide-isomerase erp38 OS=Neurospora crassa GN=erp38 PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 3.906e-7
Identity = 24/58 (41.38%), Postives = 36/58 (62.07%), Query Frame = -2

Query:  395 KGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQ 568
            K KV++  VD DA+++L  RF VQGFPT+  F    + PV Y+G R   ++ +F  E+
Sbjct:   71 KDKVQIAKVDADAERALGKRFGVQGFPTLKFFDGKSEQPVDYKGGRDLDSLSNFIAEK 128

[Close]

BLAST of GH719897 vs. TAIR peptide
Match: AT1G04980.1 (| Symbols: ATPDIL2-2, ATPDI10, PDI10, PDIL2-2 | PDI-like 2-2 | chr1:1413869-1416120 REVERSE LENGTH=447)

HSP 1 Score: 305.834 bits (782), Expect = 1.031e-83
Identity = 143/191 (74.87%), Postives = 169/191 (88.48%), Query Frame = -2

Query:    5 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 577
            NNLKGKVKLGHV+CDA++S+ SRF VQGFPTILVFG+DK +PVPYEGAR+ASAIESFALEQLE+N  P EVTEL   D++E+KCGSAAICFV+FLPDILDSKAEGRNKYL+ LL+VA++FK+ PY + WVAAGKQPDLE+ VGVGGYGYPA+VALN KK  YAPLKS FE+  + +FVKEA +GG+GNLP+
Sbjct:  212 NNLKGKVKLGHVNCDAEQSIKSRFKVQGFPTILVFGSDKSSPVPYEGARSASAIESFALEQLESNAGPAEVTELTGPDVMEDKCGSAAICFVSFLPDILDSKAEGRNKYLEMLLSVADKFKKDPYGFVWVAAGKQPDLEKRVGVGGYGYPAMVALNAKKGAYAPLKSGFEVKHLKDFVKEAAKGGKGNLPI 402

HSP 2 Score: 54.299 bits (129), Expect = 5.408e-8
Identity = 27/63 (42.86%), Postives = 40/63 (63.49%), Query Frame = -2

Query:  389 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLE 577
            + LKG   +  +D DA KS+   + V+GFPTI VF   K  P+ Y+GAR A +I  FA++Q++
Sbjct:   77 STLKGIATVAAIDADAHKSVSQDYGVRGFPTIKVFVPGK-PPIDYQGARDAKSISQFAIKQIK 138

[Close]

BLAST of GH719897 vs. TAIR peptide
Match: AT2G32920.1 (| Symbols: ATPDIL2-3, PDI9, ATPDI9, PDIL2-3 | PDI-like 2-3 | chr2:13962502-13965406 REVERSE LENGTH=440)

HSP 1 Score: 288.886 bits (738), Expect = 1.305e-78
Identity = 135/190 (71.05%), Postives = 165/190 (86.84%), Query Frame = -2

Query:    5 NLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLETNVAPPEVTELYSADILEEKCGSAAICFVAFLPDILDSKAEGRNKYLQQLLAVAEQFKRSPYSYAWVAAGKQPDLEQNVGVGGYGYPALVALNIKKAVYAPLKSAFELDQIIEFVKEAGRGGQGNLPL 574
            NL+GKVKLGHV+CD ++S+MSRF VQGFPTILVFG DK +P PYEGAR+ASAIESFA E +E++  P EVTEL   D++E+KCGSAAICF++FLPDILDSKAEGRNKYL+ LL+VAE+FK+ PYS+ WVAA  Q DLE+ V VGGYGYPA+VA+N+KK VYAPLKSAFEL  ++EFVK+AG GG+GN+P+
Sbjct:  208 NLQGKVKLGHVNCDVEQSIMSRFKVQGFPTILVFGPDKSSPYPYEGARSASAIESFASELVESSAGPVEVTELTGPDVMEKKCGSAAICFISFLPDILDSKAEGRNKYLEMLLSVAEKFKKQPYSFMWVAAVTQMDLEKRVNVGGYGYPAMVAMNVKKGVYAPLKSAFELQHLLEFVKDAGTGGKGNVPM 397

HSP 2 Score: 51.9878 bits (123), Expect = 2.684e-7
Identity = 26/63 (41.27%), Postives = 39/63 (61.90%), Query Frame = -2

Query:  389 NNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPVPYEGARTASAIESFALEQLE 577
            N LKG   +  +D DA +S    + ++GFPTI VF   K  P+ Y+GAR A +I +FA +Q++
Sbjct:   75 NILKGVATVAAIDADAHQSAAQDYGIKGFPTIKVFVPGK-APIDYQGARDAKSIANFAYKQIK 136

The following BLAST results are available for this feature:

BLAST of GH719897 vs. TrEMBL
BLAST of GH719897 vs. SwissProt
BLAST of GH719897 vs. TAIR peptide

BLAST of GH719897 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T7D9_SOYBN	1.944e-96	91.10	Putative uncharacterized protein OS=Glycine max PE...	[more]
A9CPA7_SOYBN	1.944e-96	91.10	Protein disulfide isomerase family OS=Glycine max ...	[more]
B9IAL5_POPTR	6.914e-94	87.43	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7TEF8_VITVI	2.905e-92	86.91	Whole genome shotgun sequence of line PN40024, sca...	[more]
C0PPU0_PICSI	1.182e-85	78.53	Putative uncharacterized protein OS=Picea sitchens...	[more]
Q67UF5_ORYSJ	2.912e-84	78.42	Os09g0451500 protein OS=Oryza sativa subsp. japoni...	[more]
Q5EUD5_MAIZE	2.725e-82	76.84	Protein disulfide isomerase OS=Zea mays GN=PDIL2-3...	[more]
B8BCJ0_ORYSI	1.036e-81	73.53	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q9MAU6_ARATH	1.353e-81	74.87	Disulfide isomerase-related protein, putative OS=A...	[more]
D7KE98_ARALY	2.551e-80	73.82	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of GH719897 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
PDIA6_RAT	3.870e-39	42.86	Protein disulfide-isomerase A6 OS=Rattus norvegicu...	[more]
PDIA6_PONAB	3.870e-39	42.86	Protein disulfide-isomerase A6 OS=Pongo abelii GN=...	[more]
PDIA6_MOUSE	3.870e-39	42.86	Protein disulfide-isomerase A6 OS=Mus musculus GN=...	[more]
PDIA6_HUMAN	3.870e-39	42.86	Protein disulfide-isomerase A6 OS=Homo sapiens GN=...	[more]
PDIA6_MESAU	3.066e-36	40.74	Protein disulfide-isomerase A6 OS=Mesocricetus aur...	[more]
PDIA6_CAEEL	6.394e-34	39.78	Probable protein disulfide-isomerase A6 OS=Caenorh...	[more]
Y5025_DICDI	2.890e-10	44.29	Putative protein disulfide-isomerase DDB_G0275025 ...	[more]
ERP38_NEUCR	3.906e-7	41.38	Protein disulfide-isomerase erp38 OS=Neurospora cr...	[more]

BLAST of GH719897 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G04980.1	1.031e-83	74.87	\| Symbols: ATPDIL2-2, ATPDI10, PDI10, PDIL2-2 \| PD...	[more]
AT2G32920.1	1.305e-78	71.05	\| Symbols: ATPDIL2-3, PDI9, ATPDI9, PDIL2-3 \| PDI-...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012335	Thioredoxin fold	GENE3D	3.40.30.10	no description	coord: 2..65 score: 9.2
IPR012336	Thioredoxin-like fold	SUPERFAMILY	52833	Thioredoxin-like	coord: 3..62 score: 4.3e-13coord: 64..184 score: 1
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 2..58 score: 8.8
None	No IPR available	PANTHER	PTHR18929	PROTEIN DISULFIDE ISOMERASE	coord: 1..70 score: 6.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719897 ID=GH719897; Name=GH719897; organism=Pisum sativum; type=EST; length=578bp
CTCCCAAGGGCAAGTTCCCTTGGCCGCCGCGTCCAGCTTCTTTCACAAAT
TCTATAATTTGGTCAAGTTCAAAAGCACTCTTGAGAGGAGCATAAACAGC
TTTCTTAATGTTCAAGGCTACTAAAGCTGGATAACCATACCCACCAACAC
CAACATTCTGCTCAAGATCTGGCTGCTTACCGGCGGCTACCCATGCGTAG
CTGTAAGGGCTCCTTTTGAACTGCTCTGCAACGGCTAATAACTGCTGAAG
GTATTTGTTCCTCCCCTCAGCCTTGGAATCCAAAATGTCGGGAAGGAAGG
CAACAAAACAGATAGCTGCAGAACCACATTTCTCTTCCAAAATATCTGCA
CTATATAGCTCTGTCACTTCTGGAGGGGCAACATTTGTTTCGAGCTGTTC
TAATGCGAATGATTCGATAGCTGAGGCAGTTCTTGCACCCTCGTAAGGAA
CTGGAGTATCTTTATCGGCTCCAAATACCAATATTGTTGGGAATCCTTGA
ACATTGAACCTGCTCATTAGAGACTTATCAGCATCACAGTCAACATGACC
CAGTTTGACCTTCCCTTTCAAATTATTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0045454	cell redox homeostasis

Vocabulary: **INTERPRO**
Term	Definition
IPR012335	Thioredoxin fold
IPR012336	Thioredoxin-like_fold
IPR013766	Thioredoxin_domain

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