GH720022

EST Overview


Name	GH720022
Unique Name	GH720022
Type	EST
Organism	Pisum sativum (pea)
Sequence length	593

Libraries


Library Name	Type
LIBEST_024269 TNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720022 vs. TrEMBL
Match: C6SZN6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 8.527e-26
Identity = 56/73 (76.71%), Postives = 70/73 (95.89%), Query Frame = -3

Query:  343 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 561
            +LDTA+ +KQVTVVAK+YWGDAREKI E+VGDLKLD+LVMGSRGLGA++RVL+GSV+ YVT+NA+CP+TIVK+
Sbjct:   86 LLDTASREKQVTVVAKLYWGDAREKIVEAVGDLKLDSLVMGSRGLGAIQRVLLGSVTNYVTTNASCPITIVKD 158

[Close]

BLAST of GH720022 vs. TrEMBL
Match: C6SVX3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.454e-25
Identity = 56/73 (76.71%), Postives = 69/73 (94.52%), Query Frame = -3

Query:  343 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 561
            +LDTA+ QKQV VVAK+YWGDAREKI E+VGDLKLD+LVMGSRGLGA++RVL+GSV+ YVT+NA+CP+TIVK+
Sbjct:   86 LLDTASRQKQVNVVAKLYWGDAREKIVEAVGDLKLDSLVMGSRGLGAIQRVLLGSVTNYVTANASCPITIVKD 158

[Close]

BLAST of GH720022 vs. TrEMBL
Match: A9NPB4_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.100e-24
Identity = 54/73 (73.97%), Postives = 65/73 (89.04%), Query Frame = -3

Query:  343 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 561
            +LDTAA QK++ V+AKVYWGDAREK+C++V DLKLD+LVMGSRGLG +KRV +GSVS YV SNA CPVT+VKE
Sbjct:   87 MLDTAARQKELEVIAKVYWGDAREKLCDAVEDLKLDSLVMGSRGLGQLKRVFLGSVSNYVISNANCPVTVVKE 159

[Close]

BLAST of GH720022 vs. TrEMBL
Match: C5YZ64_SORBI (Putative uncharacterized protein Sb09g022230 OS=Sorghum bicolor GN=Sb09g022230 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 2.743e-24
Identity = 51/72 (70.83%), Postives = 67/72 (93.06%), Query Frame = -3

Query:  346 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 561
            ++DTAA QKQ+ VVAK+YWGDAREK+C++VGDLK+D+LVMGSRGLG ++R+L+GSV+ YV SNA+CPVT+VK
Sbjct:   93 MIDTAARQKQLKVVAKLYWGDAREKLCDAVGDLKIDSLVMGSRGLGPIQRILLGSVTNYVLSNASCPVTVVK 164

[Close]

BLAST of GH720022 vs. TrEMBL
Match: A9NQ10_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 4.679e-24
Identity = 53/73 (72.60%), Postives = 65/73 (89.04%), Query Frame = -3

Query:  343 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 561
            +LDTA  QK++ V+AKVYWGDAREK+C++V DLKLD+LVMGSRGLG +KRV +GSVS YV SNA+CPVT+VKE
Sbjct:   87 MLDTATRQKELEVIAKVYWGDAREKLCDAVEDLKLDSLVMGSRGLGQLKRVFLGSVSNYVISNASCPVTVVKE 159

[Close]

BLAST of GH720022 vs. TrEMBL
Match: B9S3A2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0731860 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 6.111e-24
Identity = 52/78 (66.67%), Postives = 65/78 (83.33%), Query Frame = -3

Query:  343 PAPRQVLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 576
            P    +LDT + QKQVT+VAK+YWGDAR++ CE+VG LKLD LVMGSRGLG +KRVL+GSV+ YV + ATCPVT+VK+
Sbjct:   81 PEVLDMLDTVSRQKQVTIVAKLYWGDARDRFCEAVGHLKLDCLVMGSRGLGTIKRVLLGSVTNYVMATATCPVTVVKD 158

[Close]

BLAST of GH720022 vs. TrEMBL
Match: B0YQX0_GOSAR (Universal stress protein 1 OS=Gossypium arboreum GN=USP1 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.981e-24
Identity = 55/78 (70.51%), Postives = 65/78 (83.33%), Query Frame = -3

Query:  343 PAPRQVLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 576
            P    ++D A+ QKQ T+VAK+YWGDAR+KICESV DLKLD LVMGSRGLG ++RVL+GSVS YV  NATCPVTIVK+
Sbjct:   81 PEILDLVDIASGQKQGTLVAKIYWGDARDKICESVEDLKLDCLVMGSRGLGTIQRVLIGSVSNYVMVNATCPVTIVKD 158

[Close]

BLAST of GH720022 vs. TrEMBL
Match: B6TYX3_MAIZE (USP family protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.756e-23
Identity = 49/71 (69.01%), Postives = 65/71 (91.55%), Query Frame = -3

Query:  346 LDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 558
            +DTAA QKQ+ VVAK+YWGDAREK+C++V DL++D+LVMGSRGLG ++R+L+GSV+ YV SNA+CPVT+VK
Sbjct:   92 IDTAARQKQLKVVAKLYWGDAREKLCDAVEDLRIDSLVMGSRGLGPIQRILLGSVTNYVLSNASCPVTVVK 162

[Close]

BLAST of GH720022 vs. TrEMBL
Match: B4G087_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.756e-23
Identity = 49/71 (69.01%), Postives = 65/71 (91.55%), Query Frame = -3

Query:  346 LDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 558
            +DTAA QKQ+ VVAK+YWGDAREK+C++V DL++D+LVMGSRGLG ++R+L+GSV+ YV SNA+CPVT+VK
Sbjct:   92 IDTAARQKQLKVVAKLYWGDAREKLCDAVEDLRIDSLVMGSRGLGPIQRILLGSVTNYVLSNASCPVTVVK 162

[Close]

BLAST of GH720022 vs. TrEMBL
Match: A5C9Q0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034082 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 8.824e-23
Identity = 49/72 (68.06%), Postives = 64/72 (88.89%), Query Frame = -3

Query:  343 LDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 558
            LDTA+ QK+V +V K+YWGDAR+K+CE+V DLKLD+LVMGSRGL  ++R+L+GSV+ YV +NATCPVTIVK+
Sbjct:   87 LDTASRQKEVKIVTKLYWGDARDKLCEAVEDLKLDSLVMGSRGLSTIRRILLGSVTNYVMTNATCPVTIVKD 158

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G03270.2 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:762136-763075 REVERSE LENGTH=159)

HSP 1 Score: 107.071 bits (266), Expect = 7.393e-24
Identity = 49/77 (63.64%), Postives = 65/77 (84.42%), Query Frame = -3

Query:  346 PAPRQVLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 576
            P    VLDT +  K+V VVAKVYWGD REK+C++V +LKLD++V+GSRGLG++KR+L+GSVS +V +NATCPVT+VK
Sbjct:   81 PEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKLDSIVLGSRGLGSLKRILLGSVSNHVVTNATCPVTVVK 157

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G53990.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:19989658-19991019 REVERSE LENGTH=160)

HSP 1 Score: 100.523 bits (249), Expect = 6.920e-22
Identity = 45/73 (61.64%), Postives = 63/73 (86.30%), Query Frame = -3

Query:  343 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVKE 561
            +LDT + QK+V VV K+YWGDAREK+ ++V DLKLD++VMGSRGL A++R++MGSVS++V  +A CPVT+VK+
Sbjct:   86 MLDTGSRQKEVHVVTKLYWGDAREKLVDAVKDLKLDSIVMGSRGLSALQRIIMGSVSSFVIQHAPCPVTVVKD 158

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G17020.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:5802728-5804063 REVERSE LENGTH=163)

HSP 1 Score: 94.3597 bits (233), Expect = 4.959e-20
Identity = 41/72 (56.94%), Postives = 57/72 (79.17%), Query Frame = -3

Query:  346 VLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 561
            +++TAA +K +TVV K+YWGD REKIC +   + L +LVMG+RGLG +KR++MGSVS +V +N  CPVT+VK
Sbjct:   89 IVNTAARKKTITVVMKIYWGDPREKICAAAEQIPLSSLVMGNRGLGGLKRMIMGSVSNHVVNNVACPVTVVK 160

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G03270.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:761383-763075 REVERSE LENGTH=201)

HSP 1 Score: 73.9442 bits (180), Expect = 6.936e-14
Identity = 34/55 (61.82%), Postives = 45/55 (81.82%), Query Frame = -3

Query:  412 PAPRQVLDTAADQKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKR 576
            P    VLDT +  K+V VVAKVYWGD REK+C++V +LKLD++V+GSRGLG++KR
Sbjct:   81 PEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKLDSIVLGSRGLGSLKR 135

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT1G09740.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr1:3154603-3155834 FORWARD LENGTH=171)

HSP 1 Score: 70.8626 bits (172), Expect = 5.872e-13
Identity = 34/65 (52.31%), Postives = 45/65 (69.23%), Query Frame = -3

Query:  346 QKQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 540
            +K V V  +V  GD + KICE+V +L  D LVMGSR  G +KR+ +GSVS Y T++A CPV I+K
Sbjct:  100 EKSVNVKTQVVIGDPKYKICEAVENLHADLLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIK 164

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G11930.4 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:3776371-3777393 FORWARD LENGTH=201)

HSP 1 Score: 68.1662 bits (165), Expect = 3.806e-12
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = -3

Query:  346 KQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 537
            KQ+     V  G+A+E ICE+V  + +D LV+GSRGLG +KR  +GSVS Y   +A CP+ IVK
Sbjct:  131 KQIRTETLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 194

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G11930.2 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:3776371-3777393 FORWARD LENGTH=200)

HSP 1 Score: 68.1662 bits (165), Expect = 3.806e-12
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = -3

Query:  346 KQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 537
            KQ+     V  G+A+E ICE+V  + +D LV+GSRGLG +KR  +GSVS Y   +A CP+ IVK
Sbjct:  130 KQIRTETLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 193

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G11930.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:3776371-3777393 FORWARD LENGTH=199)

HSP 1 Score: 68.1662 bits (165), Expect = 3.806e-12
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = -3

Query:  346 KQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 537
            KQ+     V  G+A+E ICE+V  + +D LV+GSRGLG +KR  +GSVS Y   +A CP+ IVK
Sbjct:  129 KQIRTETLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 192

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G58450.2 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:21622032-21623057 FORWARD LENGTH=197)

HSP 1 Score: 63.1586 bits (152), Expect = 1.224e-10
Identity = 29/64 (45.31%), Postives = 40/64 (62.50%), Query Frame = -3

Query:  346 KQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 537
            K V     +  GD +E IC++V    +D LV+GSRGLG +KR  +GSVS Y   +A CP+ IV+
Sbjct:  118 KMVKTETMILEGDPKEMICQAVEQTHVDLLVVGSRGLGMIKRAFLGSVSDYCAQHAKCPILIVR 181

[Close]

BLAST of GH720022 vs. TAIR peptide
Match: AT3G58450.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:21622032-21623057 FORWARD LENGTH=204)

HSP 1 Score: 63.1586 bits (152), Expect = 1.224e-10
Identity = 29/64 (45.31%), Postives = 40/64 (62.50%), Query Frame = -3

Query:  346 KQVTVVAKVYWGDAREKICESVGDLKLDALVMGSRGLGAVKRVLMGSVSTYVTSNATCPVTIVK 537
            K V     +  GD +E IC++V    +D LV+GSRGLG +KR  +GSVS Y   +A CP+ IV+
Sbjct:  125 KMVKTETMILEGDPKEMICQAVEQTHVDLLVVGSRGLGMIKRAFLGSVSDYCAQHAKCPILIVR 188

The following BLAST results are available for this feature:

BLAST of GH720022 vs. TrEMBL
BLAST of GH720022 vs. TAIR peptide

BLAST of GH720022 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SZN6_SOYBN	8.527e-26	76.71	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SVX3_SOYBN	1.454e-25	76.71	Putative uncharacterized protein OS=Glycine max PE...	[more]
A9NPB4_PICSI	2.100e-24	73.97	Putative uncharacterized protein OS=Picea sitchens...	[more]
C5YZ64_SORBI	2.743e-24	70.83	Putative uncharacterized protein Sb09g022230 OS=So...	[more]
A9NQ10_PICSI	4.679e-24	72.60	Putative uncharacterized protein OS=Picea sitchens...	[more]
B9S3A2_RICCO	6.111e-24	66.67	Putative uncharacterized protein OS=Ricinus commun...	[more]
B0YQX0_GOSAR	7.981e-24	70.51	Universal stress protein 1 OS=Gossypium arboreum G...	[more]
B6TYX3_MAIZE	6.756e-23	69.01	USP family protein OS=Zea mays PE=2 SV=1	[more]
B4G087_MAIZE	6.756e-23	69.01	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
A5C9Q0_VITVI	8.824e-23	68.06	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of GH720022 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G03270.2	7.393e-24	63.64	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G53990.1	6.920e-22	61.64	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G17020.1	4.959e-20	56.94	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G03270.1	6.936e-14	61.82	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT1G09740.1	5.872e-13	52.31	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G11930.4	3.806e-12	50.00	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G11930.2	3.806e-12	50.00	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G11930.1	3.806e-12	50.00	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G58450.2	1.224e-10	45.31	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G58450.1	1.224e-10	45.31	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 64..71 score
None	No IPR available	SEG	seg	seg	coord: 23..35 score: NAcoord: 41..48 score
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 9..82 score: 9.5
IPR006015	Universal stress protein A	PRINTS	PR01438	UNVRSLSTRESS	coord: 42..54 score: 3.8e-10coord: 60..82 score: 3.8
IPR006016	UspA	PFAM	PF00582	Usp	coord: 14..82 score: 2.2
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	no description	coord: 14..82 score: 2.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720022 ID=GH720022; Name=GH720022; organism=Pisum sativum; type=EST; length=593bp
CCATAAGAAATAGTTAAAGAAAAACAACCTCATGAAGATGAACACAAACA
AAACACAGAATTTGCATAAAGCAACACTTTGATGATTATTTTACAGACAA
ATACACAGTGAGAATCAAACATGAAACATGTCAATCCACTCCATCATGTT
AGGTAGCATATTTGAAACAGATCCACCACAAAGCTTAGTTATAAACACAC
TTTTGGCTTTAGCATAAGCACTTATATAAACTAAAAATAGTGTCTTAAAA
AACCAGAGTCTAATAGATCATAAATACGTAAATGTAAGAACAAAGAAAAC
AGCCATTTGCAAAGAGATCAACCAAACACAGCAGCATACTCATTCTTTCA
CAATTGTTACTGGACAAGTTGCATTTGAAGTGACATAAGTAGACACACTC
CCCATCAATACCCTCTTAACGGCACCCAAACCTCTACTACCCATGACCAA
AGCATCAAGCTTCAAATCTCCAACAGACTCACAAATTTTCTCTCTTGCAT
CTCCCCAATAAACCTTCGCCACCACAGTCACCTGTTTCTGATCAGCCGCA
GTATCGAGTACCTGCCGGGGGGCGGGGGGGGCGAGGGGGGCCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006950	response to stress

Vocabulary: **INTERPRO**
Term	Definition
IPR006015	Universal_stress_UspA
IPR006016	UspA
IPR014729	Rossmann-like_a/b/a_fold

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