GH720075

EST Overview


Name	GH720075
Unique Name	GH720075
Type	EST
Organism	Pisum sativum (pea)
Sequence length	560

Libraries


Library Name	Type
LIBEST_014004 TNE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720075 vs. TrEMBL
Match: Q9FVI4_LOTJA (Putative ammonium transporter AMT1;1 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.134e-61
Identity = 122/172 (70.93%), Postives = 129/172 (75.00%), Query Frame = 3

Query:   45 MSLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            M+LPECSA  LAQLIGPNAT+A A A FIC QFTAVG+RF DT +AVD+TYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIG+HFFGLKDVP+ AFDYSYFLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    1 MALPECSAANLAQLIGPNATDAAAVAGFICDQFTAVGQRFSDTAFAVDSTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGFAFAFGAPSNGFIGRHFFGLKDVPTVAFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 172

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BLAST of GH720075 vs. TrEMBL
Match: Q9FSH3_LOTJA (Ammonium transporter (AMT1.1) OS=Lotus japonicus GN=amt1.1 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.191e-60
Identity = 121/171 (70.76%), Postives = 128/171 (74.85%), Query Frame = 3

Query:   48 SLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            +LPECSA  LAQLIGPNAT+A A A FIC QFTAVG+RF DT +AVD+TYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIG+HFFGLKDVP+ AFDYSYFLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 ALPECSAANLAQLIGPNATDAAAVAGFICDQFTAVGQRFSDTAFAVDSTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGFAFAFGAPSNGFIGRHFFGLKDVPTVAFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 173

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BLAST of GH720075 vs. TrEMBL
Match: B9HSW3_POPTR (Ammonium transporter OS=Populus trichocarpa GN=PtrAMT1-1 PE=4 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.666e-54
Identity = 112/167 (67.07%), Postives = 116/167 (69.46%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  LAQL+G N TN+  AADFICSQF      F  TQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH FGLK+ PS +FDYSYFLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 CSAGDLAQLLGSNVTNSTGAADFICSQFNTAADNFSATQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGFAFAFGTPSNGFIGKHNFGLKNFPSSSFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 169

[Close]

BLAST of GH720075 vs. TrEMBL
Match: B9RWT3_RICCO (Ammonium transporter, putative OS=Ricinus communis GN=RCOM_1024140 PE=4 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 8.266e-54
Identity = 110/172 (63.95%), Postives = 122/172 (70.93%), Query Frame = 3

Query:   45 MSLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            M+   CSA +LAQL+GPN T+++ AA+FICS+FTA    F  T+YAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH FGL+ +PS +FDYS FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    1 MATMACSANELAQLLGPNITSSLDAANFICSRFTASDDNFAATRYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGFAFAFGGPSNGFIGKHHFGLESIPSSSFDYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 172

[Close]

BLAST of GH720075 vs. TrEMBL
Match: A5B2L9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_044207 PE=4 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 2.405e-53
Identity = 111/172 (64.53%), Postives = 119/172 (69.19%), Query Frame = 3

Query:   45 MSLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            M+   CSA QLAQL+GPN T   AAA FIC +F+AV   F DT YAVDNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKHFF LK +P++ FDYS FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    1 MASLNCSAEQLAQLLGPNGT---AAASFICDRFSAVSNNFTDTNYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAATGGLFYYLFGFAFAFGSPSNGFIGKHFFALKSIPTKTFDYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 169

[Close]

BLAST of GH720075 vs. TrEMBL
Match: B9HKW8_POPTR (Ammonium transporter OS=Populus trichocarpa GN=PtrAMT1-3 PE=4 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 3.141e-53
Identity = 111/172 (64.53%), Postives = 117/172 (68.02%), Query Frame = 3

Query:   45 MSLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            M+   CSA  LAQL+GPN TN+  AA FICSQF      F  TQ+AVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH FGLK  PS +FDYSYFLYQW               ERTQFVAY+IYSSFLTGFV
Sbjct:    1 MASMNCSAGDLAQLLGPNVTNSTGAAAFICSQFQIAAGNFNATQFAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGFAFAFGTPSNGFIGKHNFGLKAFPSSSFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYMIYSSFLTGFV 172

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BLAST of GH720075 vs. TrEMBL
Match: Q09J80_9ROSI (Ammonium transporter OS=Citrus sinensis x Poncirus trifoliata GN=amt1 PE=2 SV=2)

HSP 1 Score: 204.142 bits (518), Expect = 5.015e-51
Identity = 106/167 (63.47%), Postives = 113/167 (67.66%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  LA  +GPNATNA +AA  ICSQ  A+  +F DT YAVD+TYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNI LTNVLD                   PSNGFIG+H F LK  P+  FDYSYFLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    9 CSADLLAGFLGPNATNASSAASLICSQLEAINNKFTDTAYAVDSTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIKLTNVLDAAAGGLFYYLFGFAFAFGTPSNGFIGRHNFALKSFPTSNFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 175

[Close]

BLAST of GH720075 vs. TrEMBL
Match: Q70KK9_LOTJA (High affinity ammonium transporter OS=Lotus japonicus GN=AMT1.3 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 6.549e-51
Identity = 106/167 (63.47%), Postives = 115/167 (68.86%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  L  L+G  A NA AAA+FICS+F A+  +F DT YAVDNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKHFFGL + PS +FDY +FLYQW               ERTQFVAYLIYSSFLTG V
Sbjct:    7 CSAADLHPLLGTGA-NATAAAEFICSRFGAISNKFTDTAYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAATGGIFFYIFGFALAFGTPSNGFIGKHFFGLNEFPSPSFDYGFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGLV 172

[Close]

BLAST of GH720075 vs. TrEMBL
Match: B9GRB5_POPTR (Ammonium transporter OS=Populus trichocarpa GN=PtrAMT1-4 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 2.489e-50
Identity = 106/172 (61.63%), Postives = 117/172 (68.02%), Query Frame = 3

Query:   45 MSLPECSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            M+   CSA+ L  L+G  A NA AAA++IC++F AV   FVDT YAVDNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGK FFGL+  PS +FDY YFLYQW               ERTQFV+YLIYSSFLTG V
Sbjct:    1 MAALTCSASDLQSLLG-GAANATAAAEYICTRFVAVSDHFVDTAYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAATGGLFYYLFGFAFAFGSPSNGFIGKQFFGLESFPSPSFDYGYFLYQWAFAIAAAGITSGSIAERTQFVSYLIYSSFLTGLV 171

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BLAST of GH720075 vs. TrEMBL
Match: D7MV56_ARALY (High-affinity ammonium transporter (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_358961 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.250e-50
Identity = 103/167 (61.68%), Postives = 116/167 (69.46%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            C+AT L+ L+GPNAT A   A++IC Q + V  +F+DT +A+D TYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH+FGLKD+P+   DYS FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 CNATDLSVLLGPNATEA---ANYICGQLSVVDSKFIDTAFAIDTTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLKDIPTATADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 166

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BLAST of GH720075 vs. SwissProt
Match: AMT11_ARATH (Ammonium transporter 1 member 1 OS=Arabidopsis thaliana GN=AMT1-1 PE=1 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 7.568e-53
Identity = 107/167 (64.07%), Postives = 118/167 (70.66%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSAT LA L+GPNAT   AAA++IC Q   V  +F+DT +A+DNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH+FGLKD+P+ + DYS FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 CSATDLAVLLGPNAT---AAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLKDIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 166

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT12_SOLLC (Ammonium transporter 1 member 2 OS=Solanum lycopersicum GN=AMT1-2 PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 1.291e-52
Identity = 108/167 (64.67%), Postives = 119/167 (71.26%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA +L   +G +A NA AAA+FICS+F+AV +   +T YAVD TYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKHFFGLK+ PS+AFDYSYFLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    7 CSAAELFPHLGSSA-NATAAAEFICSRFSAVSEYLTNTTYAVDTTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGFSYYLFGFAFAFGAPSNGFIGKHFFGLKEFPSQAFDYSYFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 172

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT11_SOLLC (Ammonium transporter 1 member 1 OS=Solanum lycopersicum GN=AMT1-1 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 4.905e-52
Identity = 106/167 (63.47%), Postives = 116/167 (69.46%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CS   LA  +GPN TNAVAAA +IC+QF+ V  RFVDT YA+D+TYLLFSAYLVFSMQLGFAML AGSVR  NTMNIMLTNVLD                   PSNGFIG+HFFGLK++PS +FDY  FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 CSVDTLAPFLGPNTTNAVAAASYICNQFSGVSDRFVDTGYAIDSTYLLFSAYLVFSMQLGFAMLLAGSVR--NTMNIMLTNVLDAAAGGLFYYLFGFAFALGGPSNGFIGRHFFGLKEIPSNSFDYMNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 167

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT14_ARATH (Ammonium transporter 1 member 4 OS=Arabidopsis thaliana GN=AMT1-4 PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 4.591e-50
Identity = 102/169 (60.36%), Postives = 112/169 (66.27%), Query Frame = 3

Query:   60 CSATQLAQLI--GPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA+ L  L+  G NAT A AAA++IC +F  V  +F D  YA+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNV+D                   PSNGFIGKHFFG+ D P   FDY YFLYQW               ERTQFVAYLIYSSFLTG V
Sbjct:    7 CSASDLIPLLSGGANATAAAAAAEYICGRFDTVAGKFTDAAYAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVIDAAAGGLFYYLFGFAFAFGSPSNGFIGKHFFGMYDFPQPTFDYPYFLYQWTFAIAAAGITSGSIAERTQFVAYLIYSSFLTGLV 175

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT13_ARATH (Ammonium transporter 1 member 3 OS=Arabidopsis thaliana GN=AMT1-3 PE=1 SV=2)

HSP 1 Score: 191.045 bits (484), Expect = 3.290e-48
Identity = 100/167 (59.88%), Postives = 110/167 (65.87%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  LA L+GPNAT   AAAD+IC Q   V  +F D  +A+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                    S GFIG+H F L+D P+   DYS+FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    7 CSAADLATLLGPNAT---AAADYICGQLGTVNNKFTDAAFAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALRDFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 170

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT15_ARATH (Putative ammonium transporter 1 member 5 OS=Arabidopsis thaliana GN=AMT1-5 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 7.330e-48
Identity = 99/167 (59.28%), Postives = 113/167 (67.66%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  L+ L+GPNAT   AAAD+IC Q  +V  +F D  YA+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                    S+GFIG+H FGL++ P+   DYS+FLYQW               ERT+FVAYLIYSSFLTGFV
Sbjct:    7 CSAADLSALLGPNAT---AAADYICGQLGSVNNKFTDAAYAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGESSDGFIGRHNFGLQNFPTLTSDYSFFLYQWAFAIAAAGITSGSIAERTKFVAYLIYSSFLTGFV 170

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT12_ARATH (Ammonium transporter 1 member 2 OS=Arabidopsis thaliana GN=AMT1-2 PE=1 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.207e-44
Identity = 97/172 (56.40%), Postives = 112/172 (65.12%), Query Frame = 3

Query:   60 CSATQLAQLIGP--NATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKH--FFGLKDVPSE-AFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  L+ L+    N+T+++AAA F+CSQ + +  +  DT YAVDNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIG+H  FF L   P     D+S+FLYQW               ERTQFVAYLIYS+FLTGFV
Sbjct:    8 CSAVDLSALLSSSSNSTSSLAAATFLCSQISNISNKLSDTTYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAFGTPSNGFIGRHHSFFALSSYPERPGSDFSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSTFLTGFV 179

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT12_ORYSJ (Ammonium transporter 1 member 2 OS=Oryza sativa subsp. japonica GN=AMT1-2 PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.917e-44
Identity = 97/165 (58.79%), Postives = 106/165 (64.24%), Query Frame = 3

Query:   66 ATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            A  L  L+G  A NA    D++C+QF        DT  AVD+TYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKHFFGLK VP   FDYS+FL+QW               ERTQFVAYLIYS+FLTGFV
Sbjct:    5 ADTLGPLLGTAAANAT---DYLCNQFA-------DTTSAVDSTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIGKHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFV 159

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT11_ORYSJ (Ammonium transporter 1 member 1 OS=Oryza sativa subsp. japonica GN=AMT1-1 PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.868e-43
Identity = 96/165 (58.18%), Postives = 105/165 (63.64%), Query Frame = 3

Query:   66 ATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            A  LA L+GP A NA    D++C+       RF DT  AVD TYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGK FFGLK +P   FDY +FL+QW               ERTQFVAYLIYS+FLTGFV
Sbjct:    5 AADLAPLLGPVAANAT---DYLCN-------RFADTTSAVDATYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGTPSNGFIGKQFFGLKHMPQTGFDYDFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFV 159

[Close]

BLAST of GH720075 vs. SwissProt
Match: AMT13_ORYSJ (Ammonium transporter 1 member 3 OS=Oryza sativa subsp. japonica GN=AMT1-3 PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 7.347e-40
Identity = 89/162 (54.94%), Postives = 103/162 (63.58%), Query Frame = 3

Query:   75 LAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            L  L+G  A N+  AA++IC++FT       DT  AVD TYLLFSAYLVF+MQLGFAMLCAGSVRAKN+MNIMLTNVLD                   PS GFIGK FFGLK +P   +DY +FL+QW               ERT+F AYLIYS+FLTGFV
Sbjct:    8 LGPLLG-GAANSTDAANYICNRFT-------DTSSAVDATYLLFSAYLVFAMQLGFAMLCAGSVRAKNSMNIMLTNVLDAAAGALFYYLFGFAFAFGTPSKGFIGKQFFGLKHMPQTGYDYDFFLFQWAFAIAAAGITSGSIAERTRFSAYLIYSAFLTGFV 161

[Close]

BLAST of GH720075 vs. TAIR peptide
Match: AT4G13510.1 (| Symbols: AMT1;1, ATAMT1, ATAMT1;1 | ammonium transporter 1;1 | chr4:7858220-7859725 FORWARD LENGTH=501)

HSP 1 Score: 206.453 bits (524), Expect = 8.102e-54
Identity = 107/167 (64.07%), Postives = 118/167 (70.66%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSAT LA L+GPNAT   AAA++IC Q   V  +F+DT +A+DNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIGKH+FGLKD+P+ + DYS FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    3 CSATDLAVLLGPNAT---AAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLKDIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 166

[Close]

BLAST of GH720075 vs. TAIR peptide
Match: AT4G28700.1 (| Symbols: AMT1;4 | ammonium transporter 1;4 | chr4:14161681-14163195 FORWARD LENGTH=504)

HSP 1 Score: 197.208 bits (500), Expect = 4.915e-51
Identity = 102/169 (60.36%), Postives = 112/169 (66.27%), Query Frame = 3

Query:   60 CSATQLAQLI--GPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA+ L  L+  G NAT A AAA++IC +F  V  +F D  YA+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNV+D                   PSNGFIGKHFFG+ D P   FDY YFLYQW               ERTQFVAYLIYSSFLTG V
Sbjct:    7 CSASDLIPLLSGGANATAAAAAAEYICGRFDTVAGKFTDAAYAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVIDAAAGGLFYYLFGFAFAFGSPSNGFIGKHFFGMYDFPQPTFDYPYFLYQWTFAIAAAGITSGSIAERTQFVAYLIYSSFLTGLV 175

[Close]

BLAST of GH720075 vs. TAIR peptide
Match: AT3G24300.1 (| Symbols: AMT1;3, ATAMT1;3 | ammonium transporter 1;3 | chr3:8805858-8807354 REVERSE LENGTH=498)

HSP 1 Score: 191.045 bits (484), Expect = 3.523e-49
Identity = 100/167 (59.88%), Postives = 110/167 (65.87%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  LA L+GPNAT   AAAD+IC Q   V  +F D  +A+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                    S GFIG+H F L+D P+   DYS+FLYQW               ERTQFVAYLIYSSFLTGFV
Sbjct:    7 CSAADLATLLGPNAT---AAADYICGQLGTVNNKFTDAAFAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALRDFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFV 170

[Close]

BLAST of GH720075 vs. TAIR peptide
Match: AT3G24290.1 (| Symbols: AMT1;5 | ammonium transporter 1;5 | chr3:8801400-8802890 REVERSE LENGTH=496)

HSP 1 Score: 189.889 bits (481), Expect = 7.847e-49
Identity = 99/167 (59.28%), Postives = 113/167 (67.66%), Query Frame = 3

Query:   60 CSATQLAQLIGPNATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKHFFGLKDVPSEAFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  L+ L+GPNAT   AAAD+IC Q  +V  +F D  YA+DNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                    S+GFIG+H FGL++ P+   DYS+FLYQW               ERT+FVAYLIYSSFLTGFV
Sbjct:    7 CSAADLSALLGPNAT---AAADYICGQLGSVNNKFTDAAYAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGESSDGFIGRHNFGLQNFPTLTSDYSFFLYQWAFAIAAAGITSGSIAERTKFVAYLIYSSFLTGFV 170

[Close]

BLAST of GH720075 vs. TAIR peptide
Match: AT1G64780.1 (| Symbols: ATAMT1;2, AMT1;2 | ammonium transporter 1;2 | chr1:24061021-24062565 REVERSE LENGTH=514)

HSP 1 Score: 178.333 bits (451), Expect = 2.363e-45
Identity = 97/172 (56.40%), Postives = 112/172 (65.12%), Query Frame = 3

Query:   60 CSATQLAQLIGP--NATNAVAAADFICSQFTAVGKRFVDTQYAVDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDXXXXXXXXXXXXXXXXXXXPSNGFIGKH--FFGLKDVPSE-AFDYSYFLYQWXXXXXXXXXXXXXXXERTQFVAYLIYSSFLTGFV 560
            CSA  L+ L+    N+T+++AAA F+CSQ + +  +  DT YAVDNTYLLFSAYLVF+MQLGFAMLCAGSVRAKNTMNIMLTNVLD                   PSNGFIG+H  FF L   P     D+S+FLYQW               ERTQFVAYLIYS+FLTGFV
Sbjct:    8 CSAVDLSALLSSSSNSTSSLAAATFLCSQISNISNKLSDTTYAVDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAFGTPSNGFIGRHHSFFALSSYPERPGSDFSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSTFLTGFV 179

The following BLAST results are available for this feature:

BLAST of GH720075 vs. TrEMBL
BLAST of GH720075 vs. SwissProt
BLAST of GH720075 vs. TAIR peptide

BLAST of GH720075 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FVI4_LOTJA	3.134e-61	70.93	Putative ammonium transporter AMT1;1 OS=Lotus japo...	[more]
Q9FSH3_LOTJA	1.191e-60	70.76	Ammonium transporter (AMT1.1) OS=Lotus japonicus G...	[more]
B9HSW3_POPTR	1.666e-54	67.07	Ammonium transporter OS=Populus trichocarpa GN=Ptr...	[more]
B9RWT3_RICCO	8.266e-54	63.95	Ammonium transporter, putative OS=Ricinus communis...	[more]
A5B2L9_VITVI	2.405e-53	64.53	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9HKW8_POPTR	3.141e-53	64.53	Ammonium transporter OS=Populus trichocarpa GN=Ptr...	[more]
Q09J80_9ROSI	5.015e-51	63.47	Ammonium transporter OS=Citrus sinensis x Poncirus...	[more]
Q70KK9_LOTJA	6.549e-51	63.47	High affinity ammonium transporter OS=Lotus japoni...	[more]
B9GRB5_POPTR	2.489e-50	61.63	Ammonium transporter OS=Populus trichocarpa GN=Ptr...	[more]
D7MV56_ARALY	3.250e-50	61.68	High-affinity ammonium transporter (Fragment) OS=A...	[more]

BLAST of GH720075 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AMT11_ARATH	7.568e-53	64.07	Ammonium transporter 1 member 1 OS=Arabidopsis tha...	[more]
AMT12_SOLLC	1.291e-52	64.67	Ammonium transporter 1 member 2 OS=Solanum lycoper...	[more]
AMT11_SOLLC	4.905e-52	63.47	Ammonium transporter 1 member 1 OS=Solanum lycoper...	[more]
AMT14_ARATH	4.591e-50	60.36	Ammonium transporter 1 member 4 OS=Arabidopsis tha...	[more]
AMT13_ARATH	3.290e-48	59.88	Ammonium transporter 1 member 3 OS=Arabidopsis tha...	[more]
AMT15_ARATH	7.330e-48	59.28	Putative ammonium transporter 1 member 5 OS=Arabid...	[more]
AMT12_ARATH	2.207e-44	56.40	Ammonium transporter 1 member 2 OS=Arabidopsis tha...	[more]
AMT12_ORYSJ	4.917e-44	58.79	Ammonium transporter 1 member 2 OS=Oryza sativa su...	[more]
AMT11_ORYSJ	1.868e-43	58.18	Ammonium transporter 1 member 1 OS=Oryza sativa su...	[more]
AMT13_ORYSJ	7.347e-40	54.94	Ammonium transporter 1 member 3 OS=Oryza sativa su...	[more]

BLAST of GH720075 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G13510.1	8.102e-54	64.07	\| Symbols: AMT1;1, ATAMT1, ATAMT1;1 \| ammonium tra...	[more]
AT4G28700.1	4.915e-51	60.36	\| Symbols: AMT1;4 \| ammonium transporter 1;4 \| chr...	[more]
AT3G24300.1	3.523e-49	59.88	\| Symbols: AMT1;3, ATAMT1;3 \| ammonium transporter...	[more]
AT3G24290.1	7.847e-49	59.28	\| Symbols: AMT1;5 \| ammonium transporter 1;5 \| chr...	[more]
AT1G64780.1	2.363e-45	56.40	\| Symbols: ATAMT1;2, AMT1;2 \| ammonium transporter...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 38..44 score
None	No IPR available	GENE3D	1.10.3430.10	no description	coord: 74..186 score: 2.1
None	No IPR available	PANTHER	PTHR11730:SF8	AMMONIUM TRANSPORTER 1	coord: 60..186 score: 3.3
None	No IPR available	SEG	seg	seg	coord: 104..122 score: NAcoord: 153..167 score
IPR001905	Ammonium transporter	PANTHER	PTHR11730	AMMONIUM TRANSPORTER	coord: 60..186 score: 3.3
IPR001905	Ammonium transporter	PFAM	PF00909	Ammonium_transp	coord: 64..186 score: 2.2
IPR001905	Ammonium transporter	SUPERFAMILY	111352	Ammonium transporter	coord: 56..186 score: 3.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720075 ID=GH720075; Name=GH720075; organism=Pisum sativum; type=EST; length=560bp
GCTTTTCTTTCCAAGTAACTTCCGTTACTCACCTACCTACCAACATGTCG
CTGCCGGAATGTTCAGCTACACAACTAGCGCAGCTCATCGGTCCCAACGC
TACAAACGCCGTAGCCGCCGCTGATTTCATCTGCAGCCAGTTTACAGCGG
TGGGAAAAAGGTTCGTTGACACACAATACGCGGTGGATAACACATATCTT
CTATTCTCAGCTTATCTTGTTTTCTCTATGCAACTTGGTTTCGCTATGCT
CTGTGCCGGTTCAGTTCGAGCCAAAAACACCATGAACATCATGCTTACAA
ACGTTCTAGACGCCGCCGCCGGTGGGCTGTTTTACTATCTCTTCGGTTTC
GCCTTTGCCTTCGGTGGACCTTCAAACGGTTTCATCGGAAAACATTTCTT
CGGCCTCAAAGACGTTCCGTCAGAAGCTTTTGATTACAGTTATTTTCTTT
ATCAGTGGGCTTTCGCCATAGCAGCCGCCGGCATCACCAGCGGCTCTATA
GCAGAACGAACACAGTTTGTTGCTTATCTTATTTACTCCTCCTTTCTCAC
TGGCTTTGTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport
GO:0055085	transmembrane transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0008519	ammonium transmembrane transporter activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR001905	Ammonium_transpt

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