FG529505

EST Overview


Name	FG529505
Unique Name	FG529505
Type	EST
Organism	Pisum sativum (pea)
Sequence length	428

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q9SC36_PEA (Putative cathepsin B-like protease (Fragment) OS=Pisum sativum GN=cat1-related PE=2 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 5.803e-73
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF
Sbjct:   56 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 182

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q2HV10_MEDTR (Peptidase C1A, papain; Somatotropin hormone; Peptidase C1, propeptide OS=Medicago truncatula GN=MtrDRAFT_AC149038g2v2 PE=3 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 1.635e-65
Identity = 107/126 (84.92%), Postives = 114/126 (90.48%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            AGW+A INPRFSN TVGQFKRLLGVKQTPR+ELSS PVVTHPKSL LPK+FDARTAW QCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHF ++V LSVND+LACCG LCG+GC GG P SAW Y
Sbjct:   54 AGWEATINPRFSNFTVGQFKRLLGVKQTPRSELSSAPVVTHPKSLKLPKDFDARTAWSQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFDMNVSLSVNDILACCGLLCGAGCAGGTPFSAWIY 179

HSP 2 Score: 39.6614 bits (91), Expect = 1.635e-65
Identity = 17/24 (70.83%), Postives = 17/24 (70.83%), Query Frame = 1

Query:  355 GIPFLHGNTFAHHGVVTEECDPYF 426
            G PF      AHHGVVTEECDPYF
Sbjct:  171 GTPFSAWIYLAHHGVVTEECDPYF 194

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q2HV09_MEDTR (Peptidase C1A, papain; Somatotropin hormone; Peptidase C1, propeptide OS=Medicago truncatula GN=MtrDRAFT_AC149038g3v2 PE=3 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.482e-60
Identity = 106/126 (84.13%), Postives = 114/126 (90.48%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            AGW+AAINPRFSN TVGQFKRLLGVKQ P+ EL S PVVTHPKSL LPKEFDARTAW QCSTIG+ILDQGHCGSCWAFGAVESL DRFCIHF +++ LSVNDLLACCGFLCG+GCDGG PI AW+Y
Sbjct:   55 AGWEAAINPRFSNFTVGQFKRLLGVKQAPKKELLSTPVVTHPKSLKLPKEFDARTAWSQCSTIGKILDQGHCGSCWAFGAVESLQDRFCIHFDMNISLSVNDLLACCGFLCGAGCDGGTPIYAWRY 180

[Close]

BLAST of FG529505 vs. TrEMBL
Match: B7FJ05_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.482e-60
Identity = 106/126 (84.13%), Postives = 114/126 (90.48%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            AGW+AAINPRFSN TVGQFKRLLGVKQ P+ EL S PVVTHPKSL LPKEFDARTAW QCSTIG+ILDQGHCGSCWAFGAVESL DRFCIHF +++ LSVNDLLACCGFLCG+GCDGG PI AW+Y
Sbjct:   57 AGWEAAINPRFSNFTVGQFKRLLGVKQAPKKELLSTPVVTHPKSLKLPKEFDARTAWSQCSTIGKILDQGHCGSCWAFGAVESLQDRFCIHFDMNISLSVNDLLACCGFLCGAGCDGGTPIYAWRY 182

[Close]

BLAST of FG529505 vs. TrEMBL
Match: B9GRU7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_552399 PE=3 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 3.303e-60
Identity = 103/127 (81.10%), Postives = 115/127 (90.55%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKA +N  FSN TV QFK LLGVK TP+ EL  IPV++HPKSL LP+EFDARTAWPQCSTIG+ILDQGHCGSCWAFGAVESLSDRFCIH+G+++ LSVNDLLACCGFLCGSGC+GGYPISAW+YF
Sbjct:   55 AGWKATMNHHFSNYTVAQFKYLLGVKPTPKEELRGIPVISHPKSLRLPEEFDARTAWPQCSTIGKILDQGHCGSCWAFGAVESLSDRFCIHYGMNISLSVNDLLACCGFLCGSGCNGGYPISAWRYF 181

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q1HER6_NICBE (Cathepsin B OS=Nicotiana benthamiana GN=CathB PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 101/127 (79.53%), Postives = 112/127 (88.19%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAA+NPRFSN TV QFKRLLGVK T + +L  IP++THPK L LP+EFDAR AWP CSTIGRILDQGHCGSCWAFGAVESLSDRFCIH+G+++ LS NDLLACCGFLCG GCDGGYP+ AWKYF
Sbjct:   54 AGWKAALNPRFSNFTVSQFKRLLGVKPTRKGDLKGIPILTHPKLLELPQEFDARVAWPNCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHYGLNISLSANDLLACCGFLCGDGCDGGYPLQAWKYF 180

[Close]

BLAST of FG529505 vs. TrEMBL
Match: C6TMR4_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.796e-59
Identity = 103/126 (81.75%), Postives = 112/126 (88.89%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            AGW+AAINPRFSN TV QFKRLLGVK  P+ EL S P ++HPK+L LPK FDARTAW QCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHF V++ LSVNDLLACCGFLCGSGCDGGYP+ AW+Y
Sbjct:   55 AGWEAAINPRFSNYTVEQFKRLLGVKPMPKKELRSTPAISHPKTLKLPKNFDARTAWSQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFDVNISLSVNDLLACCGFLCGSGCDGGYPLYAWRY 180

[Close]

BLAST of FG529505 vs. TrEMBL
Match: B7FK90_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.796e-59
Identity = 104/126 (82.54%), Postives = 112/126 (88.89%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            AGW+AAINPRFSN TVGQFKRLLGVKQ P+ EL S PVVTHPKSL LPKEFDAR AW QCSTIG+ILDQGHCGSCWAFGAVESL DRFC HF +++ LSVNDLLACCGFLCG+GCDGG PI AW+Y
Sbjct:   57 AGWEAAINPRFSNFTVGQFKRLLGVKQAPKKELLSTPVVTHPKSLKLPKEFDARAAWSQCSTIGKILDQGHCGSCWAFGAVESLQDRFCSHFDMNISLSVNDLLACCGFLCGAGCDGGTPIYAWRY 182

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q40413_NICRU (Cathepsin B-like cysteine proteinase OS=Nicotiana rustica GN=catch B PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.367e-58
Identity = 99/127 (77.95%), Postives = 110/127 (86.61%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAA+NPRFSN TV QFKRLLGVK T + +L  IP++THPK L LP+EFDAR AW  CSTIGRILDQGHCGSCWAFGAVESLSDRFCIH+G+++ LS NDL ACCGFLCG GCDGGYP+ AWKYF
Sbjct:   54 AGWKAALNPRFSNFTVSQFKRLLGVKPTRKGDLKGIPILTHPKLLELPQEFDARVAWSNCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHYGLNISLSANDLYACCGFLCGDGCDGGYPLQAWKYF 180

[Close]

BLAST of FG529505 vs. TrEMBL
Match: Q9SC37_PEA (Putative cathepsin B-like protease (Fragment) OS=Pisum sativum GN=cat1-related PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 5.273e-58
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 3

Query:   75 VKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            +KQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF
Sbjct:   40 LKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 142

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_MACFA (Cathepsin B OS=Macaca fascicularis GN=CTSB PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 7.629e-30
Identity = 66/131 (50.38%), Postives = 78/131 (59.54%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W+A  N  F N  V   KRL    LG  + P+        V   + L LP+ FDAR  WPQC TI  I DQG CGSCWAFGAVE++SDR CIH    V V +S  DLL CCG +CG GC+GGYP  AW ++
Sbjct:   41 WQAGHN--FYNVDVSYLKRLCGTFLGGPKPPQR-------VMFTEDLKLPESFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGIMCGDGCNGGYPAGAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_PONAB (Cathepsin B OS=Pongo abelii GN=CTSB PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 2.899e-29
Identity = 65/131 (49.62%), Postives = 78/131 (59.54%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W+A  N  F N  V   K+L    LG  + P+        V   + L LP+ FDAR  WPQC TI  I DQG CGSCWAFGAVE++SDR CIH    V V +S  DLL CCG +CG GC+GGYP  AW ++
Sbjct:   41 WQAGHN--FYNVDVSYLKKLCGTFLGGPKPPQR-------VMFTEDLKLPESFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_HUMAN (Cathepsin B OS=Homo sapiens GN=CTSB PE=1 SV=3)

HSP 1 Score: 126.331 bits (316), Expect = 4.945e-29
Identity = 65/131 (49.62%), Postives = 77/131 (58.78%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W+A  N  F N  +   KRL    LG  + P+        V   + L LP  FDAR  WPQC TI  I DQG CGSCWAFGAVE++SDR CIH    V V +S  DLL CCG +CG GC+GGYP  AW ++
Sbjct:   41 WQAGHN--FYNVDMSYLKRLCGTFLGGPKPPQR-------VMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_RAT (Cathepsin B OS=Rattus norvegicus GN=Ctsb PE=1 SV=2)

HSP 1 Score: 123.25 bits (308), Expect = 4.186e-28
Identity = 62/128 (48.44%), Postives = 77/128 (60.16%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPV-VTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W+A  N  F N  +   K+L G      N    +P  V   + +NLP+ FDAR  W  C TI +I DQG CGSCWAFGAVE++SDR CIH    V+V +S  DLL CCG  CG GC+GGYP  AW ++
Sbjct:   41 WQAGRN--FYNVDISYLKKLCGTVLGGPN----LPERVGFSEDINLPESFDAREQWSNCPTIAQIRDQGSCGSCWAFGAVEAMSDRICIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_CHICK (Cathepsin B OS=Gallus gallus GN=CTSB PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 4.186e-28
Identity = 59/123 (47.97%), Postives = 74/123 (60.16%), Query Frame = 3

Query:   33 FSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            F N+ +   K+L    LG  + P         V   + ++LP  FD R  WP C TI  I DQG CGSCWAFGAVE++SDR C+H    V V +S  DLL+CCGF CG GC+GGYP  AW+Y+
Sbjct:   47 FHNTDMSYVKKLCGTFLGGPKAPER-------VDFAEDMDLPDTFDTRKQWPNCPTISEIRDQGSCGSCWAFGAVEAISDRICVHTNAKVSVEVSAEDLLSCCGFECGMGCNGGYPSGAWRYW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_MOUSE (Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 1.218e-27
Identity = 63/129 (48.84%), Postives = 80/129 (62.02%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRLLG-VKQTPRNELSSIPV-VTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W+A  N  F N  +   K+L G V   P+     +P  V   + ++LP+ FDAR  W  C TIG+I DQG CGSCWAFGAVE++SDR CIH    V+V +S  DLL CCG  CG GC+GGYP  AW ++
Sbjct:   41 WQAGRN--FYNVDISYLKKLCGTVLGGPK-----LPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWSFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_BOVIN (Cathepsin B OS=Bos taurus GN=CTSB PE=1 SV=5)

HSP 1 Score: 120.553 bits (301), Expect = 2.713e-27
Identity = 62/131 (47.33%), Postives = 78/131 (59.54%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            WKA  N  F N  +   K+L    LG  + P+ +  +  VV       LP+ FDAR  WP C TI  I DQG CGSCWAFGAVE++SDR CIH    V+V +S  D+L CCG  CG GC+GG+P  AW ++
Sbjct:   41 WKAGHN--FYNVDLSYVKKLCGAILGGPKLPQRDAFAADVV-------LPESFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHSNGRVNVEVSAEDMLTCCGGECGDGCNGGFPSGAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CATB_PIG (Cathepsin B OS=Sus scrofa GN=CTSB PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.540e-25
Identity = 59/131 (45.04%), Postives = 75/131 (57.25%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL----LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFG--VDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            W A  N  F N  +   K+L    LG  + P+    +  ++       LPK FDAR  WP C TI  I DQG CGSCWAFGAVE++SDR CI     V+V +S  D+L CCG  CG GC+GG+P  AW ++
Sbjct:   41 WTAGHN--FYNVDLSYVKKLCGTFLGGPKLPQRAAFAADMI-------LPKSFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDRICIRSNGRVNVEVSAEDMLTCCGDECGDGCNGGFPSGAWNFW 162

[Close]

BLAST of FG529505 vs. SwissProt
Match: CPR4_CAEEL (Cathepsin B-like cysteine proteinase 4 OS=Caenorhabditis elegans GN=cpr-4 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 7.389e-25
Identity = 62/135 (45.93%), Postives = 78/135 (57.78%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLN-LPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCI--HFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY-----FCT 389
            WKA I     + T+ Q K+ L   +        + VV H  + + +P  FDART WP C +I  I DQ  CGSCWAF A E+ SDRFCI  +  V+  LS  D+L+CC   CG GC+GGYPI+AWKY     FCT
Sbjct:   39 WKAEIP---KDITIEQVKKRLMRTEFVAPHTPDVEVVKHDINEDTIPATFDARTQWPNCMSINNIRDQSDCGSCWAFAAAEAASDRFCIASNGAVNTLLSAEDVLSCCS-NCGYGCEGGYPINAWKYLVKSGFCT 169

[Close]

BLAST of FG529505 vs. SwissProt
Match: CYSP_SCHJA (Cathepsin B-like cysteine proteinase OS=Schistosoma japonicum GN=CATB PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 1.820e-23
Identity = 56/131 (42.75%), Postives = 78/131 (59.54%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGV-KQTPRNELSSIPVVTHPK-SLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHF--GVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKA  + RF +  +   + L+G  K+    + +  P V H   ++ +P +FD+R  WP C +I +I DQ  CGSCWAFGAVE+++DR CI    G    LS  DL++CC   CG GC GG+P  AW Y+
Sbjct:   44 AGWKADKSDRFHS--LDDARILMGARKEDAEMKRNRRPTVDHHDLNVEIPSQFDSRKKWPHCKSISQIRDQSRCGSCWAFGAVEAMTDRICIQSGGGQSAELSALDLISCCKD-CGDGCQGGFPGVAWDYW 171

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT4G01610.1 (| Symbols: | Cysteine proteinases superfamily protein | chr4:694857-696937 FORWARD LENGTH=359)

HSP 1 Score: 222.631 bits (566), Expect = 6.253e-59
Identity = 97/127 (76.38%), Postives = 110/127 (86.61%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAAIN RFSN+TV +FKRLLGVK TP+     +P+V+H  SL LPK FDARTAWPQC++IG ILDQGHCGSCWAFGAVESLSDRFCI FG+++ LSVNDLLACCGF CG GCDGGYPI+AW+YF
Sbjct:   57 AGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFLGVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILDQGHCGSCWAFGAVESLSDRFCIQFGMNISLSVNDLLACCGFRCGDGCDGGYPIAAWQYF 183

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT1G02305.1 (| Symbols: | Cysteine proteinases superfamily protein | chr1:455816-457974 FORWARD LENGTH=362)

HSP 1 Score: 218.394 bits (555), Expect = 1.179e-57
Identity = 95/127 (74.80%), Postives = 111/127 (87.40%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKA+ N RF+N+TV +FKRLLGVK TP+ E   +P+V+H  SL LPKEFDARTAW QC++IGRILDQGHCGSCWAFGAVESLSDRFCI + ++V LSVNDLLACCGFLCG GC+GGYPI+AW+YF
Sbjct:   60 AGWKASFNDRFANATVAEFKRLLGVKPTPKTEFLGVPIVSHDISLKLPKEFDARTAWSQCTSIGRILDQGHCGSCWAFGAVESLSDRFCIKYNMNVSLSVNDLLACCGFLCGQGCNGGYPIAAWRYF 186

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT4G01610.2 (| Symbols: | Cysteine proteinases superfamily protein | chr4:694857-696937 FORWARD LENGTH=359)

HSP 1 Score: 217.238 bits (552), Expect = 2.627e-57
Identity = 95/127 (74.80%), Postives = 108/127 (85.04%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAAIN RFSN+TV +FKRLLGVK TP+     +P+V+H  SL LPK FDARTAWPQC++IG IL  GHCGSCWAFGAVESLSDRFCI FG+++ LSVNDLLACCGF CG GCDGGYPI+AW+YF
Sbjct:   57 AGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFLGVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILGLGHCGSCWAFGAVESLSDRFCIQFGMNISLSVNDLLACCGFRCGDGCDGGYPIAAWQYF 183

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT1G02300.1 (| Symbols: | Cysteine proteinases superfamily protein | chr1:453288-455376 FORWARD LENGTH=379)

HSP 1 Score: 181.415 bits (459), Expect = 3.304e-50
Identity = 85/147 (57.82%), Postives = 101/147 (68.71%), Query Frame = 3

Query:    3 AGWKAAINPRFSNSTVGQFKRLLGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQ--------------------GHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKYF 383
            AGWKAA N RF+N+TV +FKRLLGV QTP+     +P+V H  SL LPKEFDARTAW  C++I RIL                      GHCGSCWAFGAVESLSDRFCI + ++V LS ND++ACCG LCG GC+GG+P+ AW YF
Sbjct:   57 AGWKAAFNDRFANATVAEFKRLLGVIQTPKTAYLGVPIVRHDLSLKLPKEFDARTAWSHCTSIRRILVGYILNNVLLWSTITLWFWFLLGHCGSCWAFGAVESLSDRFCIKYNLNVSLSANDVIACCGLLCGFGCNGGFPMGAWLYF 203

HSP 2 Score: 33.8834 bits (76), Expect = 3.304e-50
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 1

Query:  382 FAHHGVVTEECDPYF 426
            F +HGVVT+ECDPYF
Sbjct:  203 FKYHGVVTQECDPYF 217

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT5G60360.3 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282157 FORWARD LENGTH=361)

HSP 1 Score: 60.4622 bits (145), Expect = 4.110e-10
Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL-LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            +K  +N +F++ T  +F+R  LG  Q     L     VT      LP+  D    W +   +  + DQG CGSCW F    +L   +   FG  + LS   L+ C G     GC+GG P  A++Y
Sbjct:  100 YKLGVN-QFADLTWQEFQRTKLGAAQNCSATLKGSHKVTEAA---LPETKD----WREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQLVDCAGAFNNYGCNGGLPSQAFEY 216

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT5G60360.2 (| Symbols: AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=357)

HSP 1 Score: 60.4622 bits (145), Expect = 4.110e-10
Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL-LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            +K  +N +F++ T  +F+R  LG  Q     L     VT      LP+  D    W +   +  + DQG CGSCW F    +L   +   FG  + LS   L+ C G     GC+GG P  A++Y
Sbjct:  100 YKLGVN-QFADLTWQEFQRTKLGAAQNCSATLKGSHKVTEAA---LPETKD----WREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQLVDCAGAFNNYGCNGGLPSQAFEY 216

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT5G60360.1 (| Symbols: SAG2, AALP, ALP | aleurain-like protease | chr5:24280044-24282152 FORWARD LENGTH=358)

HSP 1 Score: 60.4622 bits (145), Expect = 4.110e-10
Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL-LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            +K  +N +F++ T  +F+R  LG  Q     L     VT      LP+  D    W +   +  + DQG CGSCW F    +L   +   FG  + LS   L+ C G     GC+GG P  A++Y
Sbjct:  100 YKLGVN-QFADLTWQEFQRTKLGAAQNCSATLKGSHKVTEAA---LPETKD----WREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQLVDCAGAFNNYGCNGGLPSQAFEY 216

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT3G45310.2 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=357)

HSP 1 Score: 60.077 bits (144), Expect = 5.368e-10
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL-LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            +K ++N +F++ T  +F+R  LG  Q     L     +T      +P   D    W +   +  + +QGHCGSCW F    +L   +   FG  + LS   L+ C G     GC GG P  A++Y
Sbjct:  100 YKLSLN-QFADLTWQEFQRYKLGAAQNCSATLKGSHKITEA---TVPDTKD----WREDGIVSPVKEQGHCGSCWTFSTTGALEAAYHQAFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEY 216

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT3G45310.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:16628704-16630473 REVERSE LENGTH=358)

HSP 1 Score: 60.077 bits (144), Expect = 5.368e-10
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 3

Query:    9 WKAAINPRFSNSTVGQFKRL-LGVKQTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLACCGFLCGSGCDGGYPISAWKY 380
            +K ++N +F++ T  +F+R  LG  Q     L     +T      +P   D    W +   +  + +QGHCGSCW F    +L   +   FG  + LS   L+ C G     GC GG P  A++Y
Sbjct:  100 YKLSLN-QFADLTWQEFQRYKLGAAQNCSATLKGSHKITEA---TVPDTKD----WREDGIVSPVKEQGHCGSCWTFSTTGALEAAYHQAFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEY 216

[Close]

BLAST of FG529505 vs. TAIR peptide
Match: AT2G21430.1 (| Symbols: | Papain family cysteine protease | chr2:9171964-9173301 REVERSE LENGTH=361)

HSP 1 Score: 56.9954 bits (136), Expect = 4.544e-9
Identity = 43/128 (33.59%), Postives = 65/128 (50.78%), Query Frame = 3

Query:   30 RFSNSTVGQFKRL-LGVK---QTPRNELSSIPVVTHPKSLNLPKEFDARTAWPQCSTIGRILDQGHCGSCWAFGAVESLSDRFCIHFGVDVPLSVNDLLAC---CG----FLCGSGCDGGYPISAWKY 380
            +FS+ T  +F+R  LGVK   + P+ + +  P++    + NLP+EFD    W     +  + +QG CGSCW+F    +L     +  G  V LS   L+ C   C       C SGC+GG   SA++Y
Sbjct:   95 QFSDLTRSEFRRKHLGVKGGFKLPK-DANQAPILP---TQNLPEEFD----WRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEY 214

The following BLAST results are available for this feature:

BLAST of FG529505 vs. TrEMBL
BLAST of FG529505 vs. SwissProt
BLAST of FG529505 vs. TAIR peptide

BLAST of FG529505 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SC36_PEA	5.803e-73	100.00	Putative cathepsin B-like protease (Fragment) OS=P...	[more]
Q2HV10_MEDTR	1.635e-65	84.92	Peptidase C1A, papain; Somatotropin hormone; Pepti...	[more]
Q2HV09_MEDTR	1.482e-60	84.13	Peptidase C1A, papain; Somatotropin hormone; Pepti...	[more]
B7FJ05_MEDTR	1.482e-60	84.13	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9GRU7_POPTR	3.303e-60	81.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q1HER6_NICBE	7.357e-60	79.53	Cathepsin B OS=Nicotiana benthamiana GN=CathB PE=2...	[more]
C6TMR4_SOYBN	2.796e-59	81.75	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
B7FK90_MEDTR	2.796e-59	82.54	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q40413_NICRU	2.367e-58	77.95	Cathepsin B-like cysteine proteinase OS=Nicotiana ...	[more]
Q9SC37_PEA	5.273e-58	99.03	Putative cathepsin B-like protease (Fragment) OS=P...	[more]

BLAST of FG529505 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CATB_MACFA	7.629e-30	50.38	Cathepsin B OS=Macaca fascicularis GN=CTSB PE=2 SV...	[more]
CATB_PONAB	2.899e-29	49.62	Cathepsin B OS=Pongo abelii GN=CTSB PE=2 SV=1	[more]
CATB_HUMAN	4.945e-29	49.62	Cathepsin B OS=Homo sapiens GN=CTSB PE=1 SV=3	[more]
CATB_RAT	4.186e-28	48.44	Cathepsin B OS=Rattus norvegicus GN=Ctsb PE=1 SV=2	[more]
CATB_CHICK	4.186e-28	47.97	Cathepsin B OS=Gallus gallus GN=CTSB PE=2 SV=1	[more]
CATB_MOUSE	1.218e-27	48.84	Cathepsin B OS=Mus musculus GN=Ctsb PE=1 SV=2	[more]
CATB_BOVIN	2.713e-27	47.33	Cathepsin B OS=Bos taurus GN=CTSB PE=1 SV=5	[more]
CATB_PIG	2.540e-25	45.04	Cathepsin B OS=Sus scrofa GN=CTSB PE=1 SV=1	[more]
CPR4_CAEEL	7.389e-25	45.93	Cathepsin B-like cysteine proteinase 4 OS=Caenorha...	[more]
CYSP_SCHJA	1.820e-23	42.75	Cathepsin B-like cysteine proteinase OS=Schistosom...	[more]

BLAST of FG529505 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G01610.1	6.253e-59	76.38	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT1G02305.1	1.179e-57	74.80	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT4G01610.2	2.627e-57	74.80	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT1G02300.1	3.304e-50	57.82	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT5G60360.3	4.110e-10	32.00	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT5G60360.2	4.110e-10	32.00	\| Symbols: AALP, ALP \| aleurain-like protease \| ch...	[more]
AT5G60360.1	4.110e-10	32.00	\| Symbols: SAG2, AALP, ALP \| aleurain-like proteas...	[more]
AT3G45310.2	5.368e-10	30.40	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT3G45310.1	5.368e-10	30.40	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT2G21430.1	4.544e-9	33.59	\| Symbols: \| Papain family cysteine protease \| ch...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Peptidase, cysteine peptidase active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 69..80 score
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 47..128 score: 1.8
IPR012599	Peptidase C1A, propeptide	PFAM	PF08127	Propeptide_C1	coord: 1..29 score: 4.6
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 1..127 score: 1.8
IPR015643	Peptidase C1A, cathepsin B	PANTHER	PTHR12411:SF16	CATHEPSIN B	coord: 1..127 score: 1.8
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 3..128 score: 1.4
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 2..127 score: 5.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529505 ID=FG529505; Name=FG529505; organism=Pisum sativum; type=EST; length=428bp
GGGCAGGATGGAAGGCTGCTATTAATCCTCGCTTCTCCAATTCTACGGTT
GGACAATTTAAGCGCCTTCTTGGAGTCAAACAAACGCCTAGGAATGAACT
CAGTAGTATACCTGTCGTAACTCATCCAAAATCATTAAACTTGCCAAAGG
AATTTGATGCAAGGACAGCTTGGCCTCAATGTAGCACTATTGGAAGAATT
CTAGATCAGGGTCACTGTGGTTCTTGTTGGGCATTTGGCGCTGTTGAATC
ATTATCAGACCGTTTTTGCATTCATTTTGGCGTGGATGTGCCCCTTTCTG
TTAATGACCTTCTTGCATGTTGTGGATTTCTTTGTGGGTCTGGCTGTGAT
GGTGGGTATCCCATTTCTGCATGGAAATACTTTTGCACACCATGGTGTTG
TCACTGAAGAGTGTGACCCATACTTTGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004197	cysteine-type endopeptidase activity
GO:0008234	cysteine-type peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis
GO:0050790	regulation of catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR012599	Propeptide_C1A
IPR013128	Peptidase_C1A
IPR015643	Peptidase_C1A_cathepsin-B

Search form