FG534194

EST Overview


Name	FG534194
Unique Name	FG534194
Type	EST
Organism	Pisum sativum (pea)
Sequence length	589

Libraries


Library Name	Type
LIBEST_023216 MeriT Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG534194 vs. TrEMBL
Match: C6T2K9_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.105e-62
Identity = 117/133 (87.97%), Postives = 126/133 (94.74%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQ 401
            PQT+MN+SGESVGAIVSYYKIPLKQV+ IFDD+DLPFAKLRLLPKGGHGGHNG+KSVINH KGNSGFPRLRIGIGRPPGKMDPV+FVLR FTK EREELNFTLQ GLEA+RI+LLEGFDK A FVN+AKKIEQ
Sbjct:   89 PQTYMNSSGESVGAIVSYYKIPLKQVLEIFDDLDLPFAKLRLLPKGGHGGHNGVKSVINHFKGNSGFPRLRIGIGRPPGKMDPVAFVLRTFTKHEREELNFTLQDGLEAVRILLLEGFDKSATFVNSAKKIEQ 221

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BLAST of FG534194 vs. TrEMBL
Match: B9I2R3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_823309 PE=3 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.826e-57
Identity = 107/135 (79.26%), Postives = 120/135 (88.89%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQIG 407
            PQTFMNASGESVGAIVSYYKIPLKQV++I+DD+DLPFAKLRLLPKGGHGGHNGMKSVINH KGN  FPRLRIGIGRPPGKMD  +FVLR FTKQE EEL+F  Q G+EA+RI+LLEG +K A FVN+ K +EQ+G
Sbjct:  137 PQTFMNASGESVGAIVSYYKIPLKQVLLIYDDLDLPFAKLRLLPKGGHGGHNGMKSVINHFKGNRDFPRLRIGIGRPPGKMDTANFVLRPFTKQEHEELDFMFQQGIEAVRILLLEGLNKSATFVNSTKSMEQLG 271

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BLAST of FG534194 vs. TrEMBL
Match: D7SIW8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008257001 PE=4 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 2.019e-56
Identity = 105/133 (78.95%), Postives = 121/133 (90.98%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQ 401
            PQTFMN SGESVGAIVSYYKIPLKQV+VIFDD+DLPF+KLRLLPKGGHGGHNGM+SVI+H KG+  FPRLRIGIGRPPGKMD  +FVL  F+K+EREEL+FT Q+G+EA+RI+LLEGF+K A FVNTAK +EQ
Sbjct:  172 PQTFMNVSGESVGAIVSYYKIPLKQVLVIFDDLDLPFSKLRLLPKGGHGGHNGMRSVIDHFKGSRDFPRLRIGIGRPPGKMDTANFVLSPFSKREREELDFTFQNGVEAVRILLLEGFNKSASFVNTAKSLEQ 304

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BLAST of FG534194 vs. TrEMBL
Match: B9RE24_RICCO (Peptidyl-tRNA hydrolase, putative OS=Ricinus communis GN=RCOM_1617410 PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 2.636e-56
Identity = 105/133 (78.95%), Postives = 121/133 (90.98%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQ 401
            PQTFMNASGESVGAIVSYYKIPLKQV++I+DD+DLPFAKLRLLPKGGHGGHNGM+SVI+H KG+  FPRLRIGIGRPPGKMD  +FVLR F++QE EEL+FT Q GLEA+RI+LLEGF+K A FVN+AK +EQ
Sbjct:  138 PQTFMNASGESVGAIVSYYKIPLKQVLLIYDDLDLPFAKLRLLPKGGHGGHNGMRSVIDHFKGSRDFPRLRIGIGRPPGKMDSANFVLRPFSRQEHEELDFTFQQGLEAIRILLLEGFNKSATFVNSAKSMEQ 270

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BLAST of FG534194 vs. TrEMBL
Match: Q5FAM1_BRAOL (Putative Putative Pepidyl-tRNA hydrolase family protein OS=Brassica oleracea GN=B21F5.5 PE=3 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 2.468e-54
Identity = 101/134 (75.37%), Postives = 120/134 (89.55%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMNASGESVG IVS+YKIPLKQV+VI+DD+DLPF KLRLLPKGGHGGHNGM+S+I+ LKG+  FPRLRIGIGRPPGKMD  ++VLRQF KQE+EEL++T Q GLEA+RI+LLEGF+K A FVNT K ++Q+
Sbjct:  157 PQTFMNASGESVGQIVSFYKIPLKQVLVIYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANYVLRQFNKQEQEELDYTFQTGLEAIRIMLLEGFNKSATFVNTQKSMQQL 290

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BLAST of FG534194 vs. TrEMBL
Match: D5AB21_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 7.180e-54
Identity = 101/130 (77.69%), Postives = 116/130 (89.23%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQTFMNASGESVG +V+YYKIPL QV++IFDDMDLPFAKLRLLPKGGHGGHNGMKSVI+H KGN  FPRLRIGIGRPPGKMDP+S+VLR FTK E+EEL+ TLQ GL+A+RI++ +GFDK A  VN A+K
Sbjct:  121 PQTFMNASGESVGPLVAYYKIPLNQVLLIFDDMDLPFAKLRLLPKGGHGGHNGMKSVIDHFKGNREFPRLRIGIGRPPGKMDPISYVLRPFTKGEQEELDLTLQDGLDAIRILINQGFDKSATIVNRAQK 250

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BLAST of FG534194 vs. TrEMBL
Match: D7KGE1_ARALY (Peptidyl-tRNA hydrolase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472062 PE=4 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 3.563e-53
Identity = 98/134 (73.13%), Postives = 118/134 (88.06%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMN SGESVG IVS+YKIPLKQV+V++DD+DLPF KLRLLPKGGHGGHNGM+S+I+ LKG+  FPRLRIGIGRPPGKMD  +FVLRQF +QE+EEL++T Q G+EA+RI+LLEG +K A FVNT K +EQ+
Sbjct:  147 PQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDYTFQTGIEAIRILLLEGINKSATFVNTRKSMEQL 280

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BLAST of FG534194 vs. TrEMBL
Match: B8A808_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_03361 PE=3 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 1.354e-52
Identity = 97/134 (72.39%), Postives = 116/134 (86.57%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMNASGESVG +VSY+KIPL QV+V++DD+DLPFAKLRLLPKGGHGGHNGM+S+INHLK N  FPRLRIGIGRPPGKMDP +FVLR F ++E+EEL+F    GLEA+RI+ LEGF+K A +VNTA+  E +
Sbjct:  115 PQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGMRSIINHLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFNKSATYVNTAQSSEML 248

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BLAST of FG534194 vs. TrEMBL
Match: C5XHG6_SORBI (Putative uncharacterized protein Sb03g031890 OS=Sorghum bicolor GN=Sb03g031890 PE=3 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 3.017e-52
Identity = 96/134 (71.64%), Postives = 117/134 (87.31%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMNASGESVG +VSY+KIPL QV+V++DD+DLPFAKLRLLPKGGHGGHNGM+S++NHLK +  FPRLRIGIGRPPGKMDP +FVLR F K+E+EEL+F L  GLEA+RI++L GF+K A +VNTA+  E +
Sbjct:  112 PQTFMNASGESVGQLVSYFKIPLNQVVVMYDDLDLPFAKLRLLPKGGHGGHNGMRSIVNHLKQSRDFPRLRIGIGRPPGKMDPANFVLRPFNKKEQEELDFALHRGLEAVRIMVLGGFNKSATYVNTAQSSEML 245

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BLAST of FG534194 vs. TrEMBL
Match: B7EPP3_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_03102 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 3.940e-52
Identity = 96/134 (71.64%), Postives = 116/134 (86.57%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMNASGESVG +VSY+KIPL QV+V++DD+DLPFAKLRLLPKGGHGGHNG++S+INHLK N  FPRLRIGIGRPPGKMDP +FVLR F ++E+EEL+F    GLEA+RI+ LEGF+K A +VNTA+  E +
Sbjct:  115 PQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGVRSIINHLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFNKSATYVNTAQSSEML 248

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BLAST of FG534194 vs. SwissProt
Match: PTHC_ARATH (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 213.001 bits (541), Expect = 8.899e-55
Identity = 100/134 (74.63%), Postives = 118/134 (88.06%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMN SGESVG IVS+YKIPLKQV+V++DD+DLPF KLRLLPKGGHGGHNGM+S+I+ LKG+  FPRLRIGIGRPPGKMD  +FVLRQF +QE+EEL+ T Q GLEA+RI+LLEGF+K A FVNT K +EQ+
Sbjct:  154 PQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

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BLAST of FG534194 vs. SwissProt
Match: PTHM_ORYSJ (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 2.863e-53
Identity = 96/134 (71.64%), Postives = 116/134 (86.57%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMNASGESVG +VSY+KIPL QV+V++DD+DLPFAKLRLLPKGGHGGHNG++S+INHLK N  FPRLRIGIGRPPGKMDP +FVLR F ++E+EEL+F    GLEA+RI+ LEGF+K A +VNTA+  E +
Sbjct:  115 PQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGVRSIINHLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFNKSATYVNTAQSSEML 248

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BLAST of FG534194 vs. SwissProt
Match: PTHM_ARATH (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.061e-35
Identity = 66/134 (49.25%), Postives = 97/134 (72.39%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQT+MN SGES G + +YYK+PL +V+V+ DDM LP   LRL  KGGHG HNG+KSV+NH +GN  F RLRIGIG+PPG+MDP +F+L++F+   RE ++  L  G++A++++L + F +  +  N  +K + +
Sbjct:   78 PQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHL 211

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BLAST of FG534194 vs. SwissProt
Match: CRS2_MAIZE (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays GN=CRS2 PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 5.077e-34
Identity = 62/130 (47.69%), Postives = 96/130 (73.85%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQ++MN SGE++G + +YY++PL+ +++I+DD  LP   LRL  KGGHG HNG+++VI HL G   FPRL IGIG PPGKMDP +F+L++F+ +ER +++  L+ G++A+R ++L+GF    +  N  +K
Sbjct:  121 PQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSGSTERFNLVQK 250

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BLAST of FG534194 vs. SwissProt
Match: CRS2B_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana GN=CRS2B PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 6.631e-34
Identity = 64/130 (49.23%), Postives = 96/130 (73.85%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQT+MN SGESVG++ S+Y++PL+ +++I+D+M LP   LRL PKGG G HNG+KSV+ HL G   FPRL IGIG+PPG MD  +F+L++F+  E++++   L+ G EA++ ++L GF++G    N  +K
Sbjct:  105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQK 234

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BLAST of FG534194 vs. SwissProt
Match: CRS2A_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana GN=CRS2A PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.806e-31
Identity = 62/130 (47.69%), Postives = 90/130 (69.23%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQ +MN SGESVG + +YY+IPL+ +++I+DDM L    LRL PKGGH  HNG+K+V  HL G  G+PRL IGIG PPG MD  +F+L++F+  ER++++  L+ G+E ++ ++ EGF       N  +K
Sbjct:  111 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQK 240

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BLAST of FG534194 vs. SwissProt
Match: CRS2_ORYSJ (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.294e-29
Identity = 56/130 (43.08%), Postives = 94/130 (72.31%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQ+++N SGE++G + +YY++PL+ ++V++D+M LP   LRL  KGGHG HNG+++V+  L  +   PRL IGIG PPGKMD  +F+L++F+ +ER +++  L+ G++A+R ++L+GF    +  N  +K
Sbjct:  124 PQSYINYSGEAIGPLAAYYQVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQK 253

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BLAST of FG534194 vs. SwissProt
Match: PTH_CHLAD (Peptidyl-tRNA hydrolase OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) GN=pth PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.066e-27
Identity = 59/118 (50.00%), Postives = 85/118 (72.03%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKI-PLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEG 353
            PQT+MN SG++V A+  +YKI P ++++VI+DD+DLPFAKLRL  +G  G HNGM+S++  L G + FPRLRIGIG+PPG+MD   +VL +FT +E   L   L    +A+ +++ EG
Sbjct:   61 PQTYMNLSGQAVAALRQWYKIDPARELLVIYDDLDLPFAKLRLRERGSAGTHNGMRSIVGQL-GTTEFPRLRIGIGQPPGQMDAADYVLSRFTPEEEAVLPEVLARVADAVEVVVSEG 177

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BLAST of FG534194 vs. SwissProt
Match: PTH_CHLSY (Peptidyl-tRNA hydrolase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) GN=pth PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.524e-27
Identity = 60/118 (50.85%), Postives = 85/118 (72.03%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKI-PLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEG 353
            PQT+MN SG++V A+ ++YKI P ++++VI+DD+DLPFAKLR+  +G  G HNGM+S++  L G + FPRLR+GIG+PPGKMD   +VL +FT  E   L   L    +A+ +IL EG
Sbjct:   61 PQTYMNLSGQAVVALCNWYKIDPARELLVIYDDLDLPFAKLRIRERGSAGTHNGMRSIVAQL-GTTEFPRLRVGIGQPPGKMDAADYVLGRFTPDEEAALPDLLGRIADAVEVILREG 177

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BLAST of FG534194 vs. SwissProt
Match: PTH_CHLAA (Peptidyl-tRNA hydrolase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) GN=pth PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.524e-27
Identity = 60/118 (50.85%), Postives = 85/118 (72.03%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKI-PLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEG 353
            PQT+MN SG++V A+ ++YKI P ++++VI+DD+DLPFAKLR+  +G  G HNGM+S++  L G + FPRLR+GIG+PPGKMD   +VL +FT  E   L   L    +A+ +IL EG
Sbjct:   61 PQTYMNLSGQAVVALCNWYKIDPARELLVIYDDLDLPFAKLRIRERGSAGTHNGMRSIVAQL-GTTEFPRLRVGIGQPPGKMDAADYVLGRFTPDEEAALPDLLGRIADAVEVILREG 177

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BLAST of FG534194 vs. TAIR peptide
Match: AT1G18440.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr1:6345994-6347686 FORWARD LENGTH=288)

HSP 1 Score: 213.001 bits (541), Expect = 9.379e-56
Identity = 100/134 (74.63%), Postives = 118/134 (88.06%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQTFMN SGESVG IVS+YKIPLKQV+V++DD+DLPF KLRLLPKGGHGGHNGM+S+I+ LKG+  FPRLRIGIGRPPGKMD  +FVLRQF +QE+EEL+ T Q GLEA+RI+LLEGF+K A FVNT K +EQ+
Sbjct:  154 PQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

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BLAST of FG534194 vs. TAIR peptide
Match: AT5G19830.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:6703383-6705125 FORWARD LENGTH=219)

HSP 1 Score: 148.673 bits (374), Expect = 2.172e-36
Identity = 66/134 (49.25%), Postives = 97/134 (72.39%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKKIEQI 404
            PQT+MN SGES G + +YYK+PL +V+V+ DDM LP   LRL  KGGHG HNG+KSV+NH +GN  F RLRIGIG+PPG+MDP +F+L++F+   RE ++  L  G++A++++L + F +  +  N  +K + +
Sbjct:   78 PQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHL 211

[Close]

BLAST of FG534194 vs. TAIR peptide
Match: AT5G16140.2 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:5270308-5271517 REVERSE LENGTH=240)

HSP 1 Score: 143.665 bits (361), Expect = 6.988e-35
Identity = 64/130 (49.23%), Postives = 96/130 (73.85%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQT+MN SGESVG++ S+Y++PL+ +++I+D+M LP   LRL PKGG G HNG+KSV+ HL G   FPRL IGIG+PPG MD  +F+L++F+  E++++   L+ G EA++ ++L GF++G    N  +K
Sbjct:  105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQK 234

[Close]

BLAST of FG534194 vs. TAIR peptide
Match: AT5G16140.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:5270308-5271517 REVERSE LENGTH=240)

HSP 1 Score: 143.665 bits (361), Expect = 6.988e-35
Identity = 64/130 (49.23%), Postives = 96/130 (73.85%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQT+MN SGESVG++ S+Y++PL+ +++I+D+M LP   LRL PKGG G HNG+KSV+ HL G   FPRL IGIG+PPG MD  +F+L++F+  E++++   L+ G EA++ ++L GF++G    N  +K
Sbjct:  105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQK 234

[Close]

BLAST of FG534194 vs. TAIR peptide
Match: AT5G38290.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:15303603-15305381 FORWARD LENGTH=246)

HSP 1 Score: 135.576 bits (340), Expect = 1.903e-32
Identity = 62/130 (47.69%), Postives = 90/130 (69.23%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQ +MN SGESVG + +YY+IPL+ +++I+DDM L    LRL PKGGH  HNG+K+V  HL G  G+PRL IGIG PPG MD  +F+L++F+  ER++++  L+ G+E ++ ++ EGF       N  +K
Sbjct:  111 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQK 240

[Close]

BLAST of FG534194 vs. TAIR peptide
Match: AT5G38290.2 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:15303491-15305381 FORWARD LENGTH=250)

HSP 1 Score: 135.576 bits (340), Expect = 1.903e-32
Identity = 62/130 (47.69%), Postives = 90/130 (69.23%), Query Frame = 3

Query:    3 PQTFMNASGESVGAIVSYYKIPLKQVIVIFDDMDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVSFVLRQFTKQEREELNFTLQHGLEAMRIILLEGFDKGAKFVNTAKK 392
            PQ +MN SGESVG + +YY+IPL+ +++I+DDM L    LRL PKGGH  HNG+K+V  HL G  G+PRL IGIG PPG MD  +F+L++F+  ER++++  L+ G+E ++ ++ EGF       N  +K
Sbjct:  115 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQK 244

The following BLAST results are available for this feature:

BLAST of FG534194 vs. TrEMBL
BLAST of FG534194 vs. SwissProt
BLAST of FG534194 vs. TAIR peptide

BLAST of FG534194 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T2K9_SOYBN	1.105e-62	87.97	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
B9I2R3_POPTR	1.826e-57	79.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SIW8_VITVI	2.019e-56	78.95	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RE24_RICCO	2.636e-56	78.95	Peptidyl-tRNA hydrolase, putative OS=Ricinus commu...	[more]
Q5FAM1_BRAOL	2.468e-54	75.37	Putative Putative Pepidyl-tRNA hydrolase family pr...	[more]
D5AB21_PICSI	7.180e-54	77.69	Putative uncharacterized protein OS=Picea sitchens...	[more]
D7KGE1_ARALY	3.563e-53	73.13	Peptidyl-tRNA hydrolase family protein OS=Arabidop...	[more]
B8A808_ORYSI	1.354e-52	72.39	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5XHG6_SORBI	3.017e-52	71.64	Putative uncharacterized protein Sb03g031890 OS=So...	[more]
B7EPP3_ORYSJ	3.940e-52	71.64	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG534194 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PTHC_ARATH	8.899e-55	74.63	Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidop...	[more]
PTHM_ORYSJ	2.863e-53	71.64	Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sa...	[more]
PTHM_ARATH	2.061e-35	49.25	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidop...	[more]
CRS2_MAIZE	5.077e-34	47.69	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2B_ARATH	6.631e-34	49.23	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2A_ARATH	1.806e-31	47.69	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2_ORYSJ	1.294e-29	43.08	Chloroplastic group IIB intron splicing facilitato...	[more]
PTH_CHLAD	2.066e-27	50.00	Peptidyl-tRNA hydrolase OS=Chloroflexus aggregans ...	[more]
PTH_CHLSY	3.524e-27	50.85	Peptidyl-tRNA hydrolase OS=Chloroflexus aurantiacu...	[more]
PTH_CHLAA	3.524e-27	50.85	Peptidyl-tRNA hydrolase OS=Chloroflexus aurantiacu...	[more]

BLAST of FG534194 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G18440.1	9.379e-56	74.63	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G19830.1	2.172e-36	49.25	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G16140.2	6.988e-35	49.23	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G16140.1	6.988e-35	49.23	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G38290.1	1.903e-32	47.69	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G38290.2	1.903e-32	47.69	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	GENE3D	3.40.50.1470	no description	coord: 1..130 score: 9.3
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 1..135 score: 2.1
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 1..123 score: 1.3
IPR001328	Peptidyl-tRNA hydrolase	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 1..129 score: 6.3
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 47..57 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG534194 ID=FG534194; Name=FG534194; organism=Pisum sativum; type=EST; length=589bp
AACCACAGACTTTTATGAATGCAAGTGGTGAATCTGTTGGGGCCATAGTT
TCTTATTACAAGATCCCATTGAAGCAAGTAATTGTGATCTTTGACGACAT
GGATTTGCCTTTTGCTAAACTGCGGCTTCTACCAAAGGGTGGACATGGAG
GTCACAATGGAATGAAAAGTGTTATTAATCACTTAAAAGGGAACTCTGGT
TTTCCTCGCTTAAGAATTGGTATTGGACGACCTCCAGGGAAAATGGATCC
TGTGTCATTCGTTCTTCGACAATTCACTAAACAGGAAAGGGAAGAACTGA
ATTTTACCTTACAACATGGATTAGAAGCCATGCGGATAATTTTGTTGGAG
GGATTTGATAAAGGCGCAAAATTTGTTAATACTGCCAAAAAGATTGAGCA
AATAGGCTGAGAGACCGCTCACTTTGGACATCCTTTTGACAACTACAAGC
AAACAGGTCTCTCTTAAAAGGACACACAAGTTATAACTTAAGCACTAAAC
TCATTCAAGCCTCCATTCATTCAAAAGAACTATGTTTGAGCAAGTAAATA
GCATTCATCCATTCTTTCAGAAAGCAGTTCAGATCCCAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004045	aminoacyl-tRNA hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR001328	Pept_tRNA_hydro
IPR018171	Pept_tRNA_hydro_CS

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