AM161731

EST Overview
NameAM161731
Unique NameAM161731
TypeEST
OrganismPisum sativum (pea)
Sequence length412
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of AM161731 vs. TrEMBL
Match: C6TJ01_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 9.322e-57
Identity = 81/91 (89.01%), Postives = 84/91 (92.31%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FST GEDRIVVG+LDLNQCLSLPDEIVG KPEV GAD TRAYLSNVCVA+ELHRNGLAY LLEKSKLVA +WGITDLYVHVA DNEPAKKL
Sbjct:  115 FSTNGEDRIVVGTLDLNQCLSLPDEIVGAKPEVIGADITRAYLSNVCVAKELHRNGLAYALLEKSKLVAYDWGITDLYVHVAVDNEPAKKL 205

HSP 2 Score: 81.6481 bits (200), Expect = 9.322e-57
Identity = 38/45 (84.44%), Postives = 43/45 (95.56%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER+SGKR+GFRRVSCINASLP+SHI+ + DDLCSS
Sbjct:   68 LAEREFEALKERVSGKRMGFRRVSCINASLPLSHIATLSDDLCSS 112
[Close]
BLAST of AM161731 vs. TrEMBL
Match: B7FFF1_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.324e-55
Identity = 78/84 (92.86%), Postives = 80/84 (95.24%), Query Frame = -1 
Query:   23 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADD 274
            +S  GEDR+VVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSK VARNWGITDLYVHVA D
Sbjct:  140 YSASGEDRVVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKSVARNWGITDLYVHVAVD 223

HSP 2 Score: 81.2629 bits (199), Expect = 1.324e-55
Identity = 38/48 (79.17%), Postives = 43/48 (89.58%), Query Frame = -3 
Query:  267 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSSFFY 410
            LAEREFEALKER+SGK   FRRVSCINASLP+SH+S++YDDLCSS  Y
Sbjct:   93 LAEREFEALKERVSGKNKAFRRVSCINASLPMSHLSSLYDDLCSSCKY 140
[Close]
BLAST of AM161731 vs. TrEMBL
Match: D7U1K2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_37.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00023749001 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.085e-48
Identity = 67/91 (73.63%), Postives = 78/91 (85.71%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            F+  GEDR+V+G+LDLNQC+SLPDEI GMKP+  GAD  RAYLSNVCVA+ELHRNGL Y L+ KSK+VA+ WGITDLYVH A DNEPAK+L
Sbjct:  151 FTHNGEDRVVIGTLDLNQCVSLPDEITGMKPQGIGADFLRAYLSNVCVAKELHRNGLGYALVAKSKMVAQEWGITDLYVHFAVDNEPAKQL 241

HSP 2 Score: 73.1738 bits (178), Expect = 3.085e-48
Identity = 34/54 (62.96%), Postives = 44/54 (81.48%), Query Frame = -3 
Query:  249 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSSFFYLWRRQD 410
            LAEREFEALKER++GKR GFRRVSCINA++P+S IS+  DDLC++  +    +D
Sbjct:  104 LAEREFEALKERVAGKREGFRRVSCINATIPLSEISSFSDDLCAACKFTHNGED 157
[Close]
BLAST of AM161731 vs. TrEMBL
Match: B9T4P8_RICCO (N-acetyltransferase, putative OS=Ricinus communis GN=RCOM_0282290 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 8.908e-48
Identity = 69/91 (75.82%), Postives = 76/91 (83.52%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            ++  GEDR+VVG+LDLNQCL LPDEI G KPE  GAD  RAYLSNVCVA+ELHR GL YEL+ KSKLVA+ WGITDLYVHVA  NEPAKKL
Sbjct:  138 YTNNGEDRVVVGTLDLNQCLRLPDEITGKKPEGIGADFLRAYLSNVCVAKELHRQGLGYELIAKSKLVAQEWGITDLYVHVAVVNEPAKKL 228

HSP 2 Score: 72.4034 bits (176), Expect = 8.908e-48
Identity = 37/54 (68.52%), Postives = 43/54 (79.63%), Query Frame = -3 
Query:  249 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSSFFYLWRRQD 410
            LAEREFEA+KERISGKR GFRRVSCINA+LP S +S+ YDDLC+   Y    +D
Sbjct:   92 LAEREFEAVKERISGKRTGFRRVSCINATLPSSQLSD-YDDLCTECKYTNNGED 144
[Close]
BLAST of AM161731 vs. TrEMBL
Match: D7MDN8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_328890 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 2.094e-43
Identity = 64/91 (70.33%), Postives = 72/91 (79.12%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS   EDR+VVGSLDLNQC  LPDEI G KPE  G D  RAYLSNVCVA+ELHRNG+ Y+L++KSK VA  WGITD+YVHV  DNE AK+L
Sbjct:  144 FSDGIEDRVVVGSLDLNQCRWLPDEIAGTKPEGIGVDFARAYLSNVCVAKELHRNGVGYKLIDKSKRVAGEWGITDMYVHVTVDNEAAKRL 234

HSP 2 Score: 68.9366 bits (167), Expect = 2.094e-43
Identity = 33/45 (73.33%), Postives = 39/45 (86.67%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER SGKR GF RV+CINA+LP+S +S+  +DLCSS
Sbjct:   97 LAEREFEALKERTSGKREGFTRVACINATLPLSQLSSSSEDLCSS 141
[Close]
BLAST of AM161731 vs. TrEMBL
Match: Q94AC8_ARATH (AT4g28030/T13J8_140 OS=Arabidopsis thaliana GN=At4g28030 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 2.095e-43
Identity = 64/91 (70.33%), Postives = 71/91 (78.02%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS   EDR+VVGSLDLNQC  LPDEI G KPE  G D  RAYLSNVCVA+ELHRNG+ Y+L++KSK VA  WGITD+YVHV  DNE AK L
Sbjct:  144 FSDGIEDRVVVGSLDLNQCRWLPDEIAGTKPEGIGVDFARAYLSNVCVAKELHRNGVGYKLIDKSKRVAGEWGITDMYVHVTVDNEAAKSL 234

HSP 2 Score: 69.707 bits (169), Expect = 2.095e-43
Identity = 32/45 (71.11%), Postives = 40/45 (88.89%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER SGKR GF RV+CINA+LP+S +S+ ++DLCS+
Sbjct:   97 LAEREFEALKERTSGKREGFTRVACINATLPLSQLSSSFEDLCSA 141
[Close]
BLAST of AM161731 vs. TrEMBL
Match: Q8LFP5_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 7.872e-43
Identity = 64/91 (70.33%), Postives = 71/91 (78.02%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS   EDR+VVGSLDLNQC  LPDEI G KPE  G D  RAYLSNVCVA+ELHRNG+ Y+L++KSK VA  WGITD+YVHV  DNE AK L
Sbjct:  144 FSDGIEDRVVVGSLDLNQCRWLPDEIAGTKPEGIGVDFARAYLSNVCVAKELHRNGVGYKLIDKSKRVAGEWGITDMYVHVTVDNEAAKSL 234

HSP 2 Score: 67.781 bits (164), Expect = 7.872e-43
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER SGKR GF RV+CINA+LP+S +S+  +DLCS+
Sbjct:   97 LAEREFEALKERTSGKREGFTRVACINATLPLSQLSSSSEDLCSA 141
[Close]
BLAST of AM161731 vs. TrEMBL
Match: B9HBN7_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_416378 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 7.882e-43
Identity = 64/91 (70.33%), Postives = 73/91 (80.22%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS  GEDR+VVG+LD+NQ +SLPDEI GMKPE       R YLSNVCVA ELHRNGL Y+L+ KSK VA+ WGITDLYVHVA +NEPAK+L
Sbjct:  126 FSENGEDRVVVGTLDVNQSMSLPDEITGMKPEGIEGQFARGYLSNVCVANELHRNGLGYDLVAKSKAVAQKWGITDLYVHVAVNNEPAKQL 216

HSP 2 Score: 65.4698 bits (158), Expect = 7.882e-43
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = -3 
Query:  279 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNI-YDDLCS 410
            LAEREFEALKERI+GKR GF RVSC+NASLP+S + ++  DDLC+
Sbjct:   78 LAEREFEALKERIAGKRTGFNRVSCLNASLPLSQLLSLPDDDLCA 122
[Close]
BLAST of AM161731 vs. TrEMBL
Match: Q84S80_ORYSJ (GCN5-related N-acetyltransferase-like OS=Oryza sativa subsp. japonica GN=B1015H11.141 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 7.448e-37
Identity = 61/87 (70.11%), Postives = 73/87 (83.91%), Query Frame = -1 
Query:    2 GEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 262
            GE+R+VVGSLDLNQCL LPDE+ G +P V+ +  TRAYLSNVCVA+EL RNGL Y L++KSK +AR WGITDLYVHVA +NE A+KL
Sbjct:  168 GEERVVVGSLDLNQCLWLPDELTGKRPGVNESSHTRAYLSNVCVAKELQRNGLGYALVDKSKKLAREWGITDLYVHVAINNEAAQKL 254

HSP 2 Score: 50.0618 bits (118), Expect = 7.448e-37
Identity = 22/45 (48.89%), Postives = 35/45 (77.78%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LA+RE++AL++RISGK + F+RVSCIN ++P+       ++LCS+
Sbjct:  117 LADREYDALQDRISGKMINFQRVSCINGTVPLLPSLVSAEELCST 161
[Close]
BLAST of AM161731 vs. TrEMBL
Match: A2YT46_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_28494 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 7.448e-37
Identity = 61/87 (70.11%), Postives = 73/87 (83.91%), Query Frame = -1 
Query:    2 GEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 262
            GE+R+VVGSLDLNQCL LPDE+ G +P V+ +  TRAYLSNVCVA+EL RNGL Y L++KSK +AR WGITDLYVHVA +NE A+KL
Sbjct:  168 GEERVVVGSLDLNQCLWLPDELTGKRPGVNESSHTRAYLSNVCVAKELQRNGLGYALVDKSKKLAREWGITDLYVHVAINNEAAQKL 254

HSP 2 Score: 50.0618 bits (118), Expect = 7.448e-37
Identity = 22/45 (48.89%), Postives = 35/45 (77.78%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LA+RE++AL++RISGK + F+RVSCIN ++P+       ++LCS+
Sbjct:  117 LADREYDALQDRISGKMINFQRVSCINGTVPLLPSLVSAEELCST 161
[Close]
BLAST of AM161731 vs. TAIR peptide
Match: AT4G28030.1 (| Symbols: | Acyl-CoA N-acyltransferases (NAT) superfamily protein | chr4:13937748-13939131 REVERSE LENGTH=274)

HSP 1 Score: 130.183 bits (326), Expect = 1.198e-45
Identity = 64/91 (70.33%), Postives = 71/91 (78.02%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS   EDR+VVGSLDLNQC  LPDEI G KPE  G D  RAYLSNVCVA+ELHRNG+ Y+L++KSK VA  WGITD+YVHV  DNE AK L
Sbjct:  144 FSDGIEDRVVVGSLDLNQCRWLPDEIAGTKPEGIGVDFARAYLSNVCVAKELHRNGVGYKLIDKSKRVAGEWGITDMYVHVTVDNEAAKSL 234

HSP 2 Score: 69.707 bits (169), Expect = 1.198e-45
Identity = 32/45 (71.11%), Postives = 40/45 (88.89%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER SGKR GF RV+CINA+LP+S +S+ ++DLCS+
Sbjct:   97 LAEREFEALKERTSGKREGFTRVACINATLPLSQLSSSFEDLCSA 141
[Close]
BLAST of AM161731 vs. TAIR peptide
Match: AT4G28030.2 (| Symbols: | Acyl-CoA N-acyltransferases (NAT) superfamily protein | chr4:13937748-13939131 REVERSE LENGTH=263)

HSP 1 Score: 105.145 bits (261), Expect = 3.538e-38
Identity = 56/91 (61.54%), Postives = 62/91 (68.13%), Query Frame = -1 
Query:    2 FSTYGEDRIVVGSLDLNQCLSLPDEIVGMKPEVSGADTTRAYLSNVCVARELHRNGLAYELLEKSKLVARNWGITDLYVHVADDNEPAKKL 274
            FS   EDR+VVGSLDLNQC  LPDEI G KPE  G D  RAYLSNVCVA+ELHRNG+ Y+L++KSK VA  W           DNE AK L
Sbjct:  144 FSDGIEDRVVVGSLDLNQCRWLPDEIAGTKPEGIGVDFARAYLSNVCVAKELHRNGVGYKLIDKSKRVAGEW-----------DNEAAKSL 223

HSP 2 Score: 69.707 bits (169), Expect = 3.538e-38
Identity = 32/45 (71.11%), Postives = 40/45 (88.89%), Query Frame = -3 
Query:  276 LAEREFEALKERISGKRVGFRRVSCINASLPVSHISNIYDDLCSS 410
            LAEREFEALKER SGKR GF RV+CINA+LP+S +S+ ++DLCS+
Sbjct:   97 LAEREFEALKERTSGKREGFTRVACINATLPLSQLSSSFEDLCSA 141
The following BLAST results are available for this feature:
BLAST of AM161731 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6TJ01_SOYBN9.322e-5789.01Putative uncharacterized protein OS=Glycine max PE... [more]
B7FFF1_MEDTR1.324e-5592.86Putative uncharacterized protein (Fragment) OS=Med... [more]
D7U1K2_VITVI3.085e-4873.63Whole genome shotgun sequence of line PN40024, sca... [more]
B9T4P8_RICCO8.908e-4875.82N-acetyltransferase, putative OS=Ricinus communis ... [more]
D7MDN8_ARALY2.094e-4370.33Putative uncharacterized protein OS=Arabidopsis ly... [more]
Q94AC8_ARATH2.095e-4370.33AT4g28030/T13J8_140 OS=Arabidopsis thaliana GN=At4... [more]
Q8LFP5_ARATH7.872e-4370.33Putative uncharacterized protein OS=Arabidopsis th... [more]
B9HBN7_POPTR7.882e-4370.33Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q84S80_ORYSJ7.448e-3770.11GCN5-related N-acetyltransferase-like OS=Oryza sat... [more]
A2YT46_ORYSI7.448e-3770.11Putative uncharacterized protein OS=Oryza sativa s... [more]
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BLAST of AM161731 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G28030.11.198e-4570.33| Symbols: | Acyl-CoA N-acyltransferases (NAT) su... [more]
AT4G28030.23.538e-3861.54| Symbols: | Acyl-CoA N-acyltransferases (NAT) su... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000182GCN5-related N-acetyltransferase (GNAT) domainPFAMPF00583Acetyltransf_1coord: 27..110
score: 2.8
IPR016181Acyl-CoA N-acyltransferaseGENE3D3.40.630.30no descriptioncoord: 29..110
score: 2.3
IPR016181Acyl-CoA N-acyltransferaseSUPERFAMILY55729Acyl-CoA N-acyltransferases (Nat)coord: 25..110
score: 2.4

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM161731 ID=AM161731; Name=AM161731; organism=Pisum sativum; type=EST; length=412bp
ACAATTTCTTTGCTGGTTCATTGTCGTCGGCAACATGGACGTATAAATCT
GTTATGCCCCAATTACGAGCAACTAACTTCGACTTTTCAAGAAGTTCATA
GGCCAATCCATTCCTATGCAACTCTCTAGCAACACACACATTACTCAGGT
AAGCCCTTGTCGTATCAGCTCCGCTAACCTCTGGCTTCATTCCTACAATT
TCATCCGGAAGACTCAAGCACTGATTAAGATCGAGAGAACCAACTACTAT
CCTGTCTTCTCCATAAGTAGAAAAATGAAGAACATAAATCATCATAGATG
TTAGATATATGAGACACAGGAAGAGAAGCATTTATGCAAGAGACTCTTCT
AAAACCCACCCTCTTCCCTGAAATGCGCTCTTTCAATGCTTCAAACTCAC
GTTCAGCCAAGT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0008080N-acetyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR000182GNAT_dom
IPR016181Acyl_CoA_acyltransferase