AM162082

EST Overview


Name	AM162082
Unique Name	AM162082
Type	EST
Organism	Pisum sativum (pea)
Sequence length	432

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of AM162082 vs. TrEMBL
Match: C6TKD5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 281.567 bits (719), Expect = 1.796e-74
Identity = 139/143 (97.20%), Postives = 142/143 (99.30%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNFIV+LLGKWKESEYGGG S+PVGGIAYY+TAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFIVDLLGKWKESEYGGGQSVPVGGIAYYVTAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

[Close]

BLAST of AM162082 vs. TrEMBL
Match: C6TBU3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 279.256 bits (713), Expect = 8.911e-74
Identity = 138/143 (96.50%), Postives = 141/143 (98.60%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF V+LLGKWKESEYGGG S+PVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFFVDLLGKWKESEYGGGQSVPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

[Close]

BLAST of AM162082 vs. TrEMBL
Match: Q9SE40_PHAVU (Putative integral membrane protein OS=Phaseolus vulgaris GN=sec61 PE=2 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 2.593e-73
Identity = 138/143 (96.50%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF V+LLGKWKESEYGGG S PVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFFVDLLGKWKESEYGGGQSAPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

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BLAST of AM162082 vs. TrEMBL
Match: A9PG45_POPTR (Putative uncharacterized protein OS=Populus trichocarpa GN=POPTRDRAFT_728284 PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 7.544e-73
Identity = 136/143 (95.10%), Postives = 139/143 (97.20%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY GG  +PVGG+AYYITAPSSLADMAANPFHALFY VFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFLVNLLGKWKESEYSGGQFVPVGGLAYYITAPSSLADMAANPFHALFYFVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

[Close]

BLAST of AM162082 vs. TrEMBL
Match: B9S8E1_RICCO (Preprotein translocase secy subunit, putative OS=Ricinus communis GN=RCOM_1251830 PE=3 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 1.287e-72
Identity = 135/143 (94.41%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY GG  IPVGG+AYY+TAP+SLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHR+SNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFLVNLLGKWKESEYSGGQFIPVGGLAYYVTAPASLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRDSNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

[Close]

BLAST of AM162082 vs. TrEMBL
Match: B9N8W6_POPTR (Sec61 transport protein OS=Populus trichocarpa GN=POPTRDRAFT_828572 PE=3 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 1.681e-72
Identity = 135/143 (94.41%), Postives = 139/143 (97.20%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY GG  +PVGG+AYYITAPSSLADMAANPFHALFY VFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFLVNLLGKWKESEYSGGQFVPVGGLAYYITAPSSLADMAANPFHALFYFVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 458

[Close]

BLAST of AM162082 vs. TrEMBL
Match: D7T5Q9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_551.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007405001 PE=4 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 2.867e-72
Identity = 134/143 (93.71%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY GG  IPVGG+AYYITAPSSLADMAANPFHALFYL+FML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRE+NLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:   25 SGNFLVNLLGKWKESEYSGGQYIPVGGLAYYITAPSSLADMAANPFHALFYLIFMLAACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHREANLQKELNRYIPTAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQ 167

[Close]

BLAST of AM162082 vs. TrEMBL
Match: B8A2Y6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 5.406e-71
Identity = 133/143 (93.01%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY G HSIPVGG+AYY+TAPSSLAD+ ANPFHALFY+VFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFLVNLLGKWKESEYSG-HSIPVGGLAYYVTAPSSLADVLANPFHALFYVVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 457

[Close]

BLAST of AM162082 vs. TrEMBL
Match: Q6ES90_ORYSJ (Putative Sec61 OS=Oryza sativa subsp. japonica GN=P0512H04.19 PE=3 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 7.061e-71
Identity = 132/143 (92.31%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY G HS+PVGG+AYY+TAPSSLAD+ ANPFHALFY+VFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  178 SGNFLVNLLGKWKESEYSG-HSVPVGGLAYYVTAPSSLADVLANPFHALFYVVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 319

[Close]

BLAST of AM162082 vs. TrEMBL
Match: Q6ES11_ORYSJ (Os09g0347700 protein OS=Oryza sativa subsp. japonica GN=P0698G06.1 PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 7.061e-71
Identity = 132/143 (92.31%), Postives = 140/143 (97.90%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLGKWKESEY G HS+PVGG+AYY+TAPSSLAD+ ANPFHALFY+VFMLSACALFSKTWIEVSGSSA+DVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFLVNLLGKWKESEYSG-HSVPVGGLAYYVTAPSSLADVLANPFHALFYVVFMLSACALFSKTWIEVSGSSAKDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 457

[Close]

BLAST of AM162082 vs. SwissProt
Match: SC61A_DICDI (Protein transport protein Sec61 subunit alpha OS=Dictyostelium discoideum GN=sec61a PE=3 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 4.126e-55
Identity = 102/141 (72.34%), Postives = 123/141 (87.23%), Query Frame = -3

Query:    2 NFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 424
            N +VNL G W+ SEY     IPV G+ YYI++P++++ + A+PFHALFY+ FML++CALFSK WIEVSGSSARDVAKQLK+QQM M GHR++++ KELNRYIPTAAAFGG+CIGALTV+ADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 NILVNLFGAWRTSEYSQ-QMIPVSGLTYYISSPNNMSAVLADPFHALFYITFMLTSCALFSKVWIEVSGSSARDVAKQLKDQQMTMKGHRDTSVIKELNRYIPTAAAFGGLCIGALTVVADFMGAIGSGTGILLAVTIIYQ 455

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BLAST of AM162082 vs. SwissProt
Match: S61A2_DANRE (Protein transport protein Sec61 subunit alpha-like 2 OS=Danio rerio GN=sec61al2 PE=2 SV=3)

HSP 1 Score: 210.305 bits (534), Expect = 2.674e-54
Identity = 99/144 (68.75%), Postives = 120/144 (83.33%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG W ++  GG   + PVGG+ YY++ P S   +  +P HA+ Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTAAAFGG+CIG L+V+ADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGTWSDTSTGGPARAYPVGGLCYYLSPPESFGTVLEDPIHAIIYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A1_DANRE (Protein transport protein Sec61 subunit alpha-like 1 OS=Danio rerio GN=sec61al1 PE=2 SV=3)

HSP 1 Score: 209.92 bits (533), Expect = 3.493e-54
Identity = 100/144 (69.44%), Postives = 120/144 (83.33%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG W ++  GG   + PVGG+ YY++ P S   +  +P HA+ Y+VFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTAAAFGG+CIG L+V+ADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLDDPVHAVIYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A2_PONAB (Protein transport protein Sec61 subunit alpha isoform 2 OS=Pongo abelii GN=SEC61A2 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 5.958e-54
Identity = 100/144 (69.44%), Postives = 121/144 (84.03%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG+W +   GG   S PVGG+ YY++ P S+  +  +P H + Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAILEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A2_MOUSE (Protein transport protein Sec61 subunit alpha isoform 2 OS=Mus musculus GN=Sec61a2 PE=2 SV=3)

HSP 1 Score: 208.764 bits (530), Expect = 7.781e-54
Identity = 100/144 (69.44%), Postives = 121/144 (84.03%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG+W +   GG   S PVGG+ YY++ P S+  +  +P H + Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A2_HUMAN (Protein transport protein Sec61 subunit alpha isoform 2 OS=Homo sapiens GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 208.764 bits (530), Expect = 7.781e-54
Identity = 100/144 (69.44%), Postives = 121/144 (84.03%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG+W +   GG   S PVGG+ YY++ P S+  +  +P H + Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A2_BOVIN (Protein transport protein Sec61 subunit alpha isoform 2 OS=Bos taurus GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 208.764 bits (530), Expect = 7.781e-54
Identity = 100/144 (69.44%), Postives = 121/144 (84.03%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG+W +   GG   S PVGG+ YY++ P S+  +  +P H + Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: SC61A_BORSA (Protein transport protein Sec61 subunit alpha OS=Boreogadus saida GN=sec61a PE=2 SV=3)

HSP 1 Score: 208.379 bits (529), Expect = 1.016e-53
Identity = 100/144 (69.44%), Postives = 119/144 (82.64%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNFIVNLLG W ++  GG   + PVGG+ Y+++ P S   +  +P HA  Y+VFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTAAAFGG+CIG L+V+ADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFIVNLLGTWSDTSTGGPARAYPVGGLCYFLSPPESFGSVLDDPVHAAIYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: SC61A_GADOC (Protein transport protein Sec61 subunit alpha OS=Gadus ogac GN=sec61a PE=2 SV=3)

HSP 1 Score: 207.994 bits (528), Expect = 1.327e-53
Identity = 100/144 (69.44%), Postives = 118/144 (81.94%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF+VNLLG W ++  GG   + PVGG+ YY + P S   +  +P HA  Y+VFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTAAAFGG+CIG L+V+ADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNFLVNLLGTWSDTSTGGPARAYPVGGLCYYFSPPESFGSVLDDPVHASIYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. SwissProt
Match: S61A1_RAT (Protein transport protein Sec61 subunit alpha isoform 1 OS=Rattus norvegicus GN=Sec61a1 PE=2 SV=2)

HSP 1 Score: 207.994 bits (528), Expect = 1.327e-53
Identity = 100/144 (69.44%), Postives = 120/144 (83.33%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGG-GHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGN +V+LLG W ++  GG   + PVGG+ YY++ P S   +  +P HA+ Y+VFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQ
Sbjct:  313 SGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQ 456

[Close]

BLAST of AM162082 vs. TAIR peptide
Match: AT1G29310.1 (| Symbols: | SecY protein transport family protein | chr1:10252662-10254526 FORWARD LENGTH=475)

HSP 1 Score: 258.84 bits (660), Expect = 8.025e-70
Identity = 129/143 (90.21%), Postives = 136/143 (95.10%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF VNLLG+WKESEY G  SIPV G+AY ITAP+S ADMAA+PFHALFY+VFML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFFVNLLGQWKESEYSG-QSIPVSGLAYLITAPASFADMAAHPFHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 457

[Close]

BLAST of AM162082 vs. TAIR peptide
Match: AT2G34250.2 (| Symbols: | SecY protein transport family protein | chr2:14462635-14464572 FORWARD LENGTH=475)

HSP 1 Score: 257.684 bits (657), Expect = 1.788e-69
Identity = 128/143 (89.51%), Postives = 136/143 (95.10%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF VNLLG+WKESEY G  SIPV G+AY ITAP+S +DMAA+PFHALFY+VFML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFFVNLLGQWKESEYSG-QSIPVSGLAYLITAPASFSDMAAHPFHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 457

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BLAST of AM162082 vs. TAIR peptide
Match: AT2G34250.1 (| Symbols: | SecY protein transport family protein | chr2:14462635-14464572 FORWARD LENGTH=475)

HSP 1 Score: 257.684 bits (657), Expect = 1.788e-69
Identity = 128/143 (89.51%), Postives = 136/143 (95.10%), Query Frame = -3

Query:    2 SGNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 430
            SGNF VNLLG+WKESEY G  SIPV G+AY ITAP+S +DMAA+PFHALFY+VFML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQ
Sbjct:  316 SGNFFVNLLGQWKESEYSG-QSIPVSGLAYLITAPASFSDMAAHPFHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQ 457

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BLAST of AM162082 vs. TAIR peptide
Match: AT1G78720.1 (| Symbols: | SecY protein transport family protein | chr1:29605629-29607510 FORWARD LENGTH=475)

HSP 1 Score: 255.758 bits (652), Expect = 6.794e-69
Identity = 126/142 (88.73%), Postives = 136/142 (95.77%), Query Frame = -3

Query:    2 GNFIVNLLGKWKESEYGGGHSIPVGGIAYYITAPSSLADMAANPFHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQ 427
            GNF+VNL+G WKESEY G  SIPVGGIAYYITAPSSLA+MA +PFHALFYLVFML+ACALFSKTWIEVSGSSA+DVA+QL+EQQMVMPGHR+SNLQKELNRYIPTAAAFGG+CIGALTVLAD MGAIGSGTGILLAVTIIYQ
Sbjct:  317 GNFLVNLIGTWKESEYSG-QSIPVGGIAYYITAPSSLAEMATHPFHALFYLVFMLAACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRDSNLQKELNRYIPTAAAFGGLCIGALTVLADLMGAIGSGTGILLAVTIIYQ 457

The following BLAST results are available for this feature:

BLAST of AM162082 vs. TrEMBL
BLAST of AM162082 vs. SwissProt
BLAST of AM162082 vs. TAIR peptide

BLAST of AM162082 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TKD5_SOYBN	1.796e-74	97.20	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TBU3_SOYBN	8.911e-74	96.50	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q9SE40_PHAVU	2.593e-73	96.50	Putative integral membrane protein OS=Phaseolus vu...	[more]
A9PG45_POPTR	7.544e-73	95.10	Putative uncharacterized protein OS=Populus tricho...	[more]
B9S8E1_RICCO	1.287e-72	94.41	Preprotein translocase secy subunit, putative OS=R...	[more]
B9N8W6_POPTR	1.681e-72	94.41	Sec61 transport protein OS=Populus trichocarpa GN=...	[more]
D7T5Q9_VITVI	2.867e-72	93.71	Whole genome shotgun sequence of line PN40024, sca...	[more]
B8A2Y6_MAIZE	5.406e-71	93.01	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q6ES90_ORYSJ	7.061e-71	92.31	Putative Sec61 OS=Oryza sativa subsp. japonica GN=...	[more]
Q6ES11_ORYSJ	7.061e-71	92.31	Os09g0347700 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of AM162082 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SC61A_DICDI	4.126e-55	72.34	Protein transport protein Sec61 subunit alpha OS=D...	[more]
S61A2_DANRE	2.674e-54	68.75	Protein transport protein Sec61 subunit alpha-like...	[more]
S61A1_DANRE	3.493e-54	69.44	Protein transport protein Sec61 subunit alpha-like...	[more]
S61A2_PONAB	5.958e-54	69.44	Protein transport protein Sec61 subunit alpha isof...	[more]
S61A2_MOUSE	7.781e-54	69.44	Protein transport protein Sec61 subunit alpha isof...	[more]
S61A2_HUMAN	7.781e-54	69.44	Protein transport protein Sec61 subunit alpha isof...	[more]
S61A2_BOVIN	7.781e-54	69.44	Protein transport protein Sec61 subunit alpha isof...	[more]
SC61A_BORSA	1.016e-53	69.44	Protein transport protein Sec61 subunit alpha OS=B...	[more]
SC61A_GADOC	1.327e-53	69.44	Protein transport protein Sec61 subunit alpha OS=G...	[more]
S61A1_RAT	1.327e-53	69.44	Protein transport protein Sec61 subunit alpha isof...	[more]

BLAST of AM162082 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G29310.1	8.025e-70	90.21	\| Symbols: \| SecY protein transport family protei...	[more]
AT2G34250.2	1.788e-69	89.51	\| Symbols: \| SecY protein transport family protei...	[more]
AT2G34250.1	1.788e-69	89.51	\| Symbols: \| SecY protein transport family protei...	[more]
AT1G78720.1	6.794e-69	88.73	\| Symbols: \| SecY protein transport family protei...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008197	Whey acidic protein, 4-disulphide core	PFAM	PF00095	WAP	coord: 43..63 score: 0.0
IPR002208	SecY protein	PANTHER	PTHR10906	SECY/SEC61-ALPHA FAMILY MEMBER	coord: 1..143 score: 2.5
IPR002208	SecY protein	PFAM	PF00344	SecY	coord: 18..143 score: 4.6
IPR023201	SecY subunit domain	GENE3D	1.10.3370.10	no description	coord: 5..143 score: 1.2
IPR023201	SecY subunit domain	SUPERFAMILY	103491	Preprotein translocase SecY subunit	coord: 2..143 score: 4.8
None	No IPR available	PANTHER	PTHR10906:SF1	PREPROTEIN TRANSLOCASE SECY SUBUNIT (SEC61)	coord: 1..143 score: 2.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162082 ID=AM162082; Name=AM162082; organism=Pisum sativum; type=EST; length=432bp
ACTGATAGATGATTGTTACTGCAAGCAATATCCCTGTCCCTGAACCTATT
GCCCCCATAAAATCTGCCAACACTGTTAGGGCACCAATACATATGCCTCC
AAATGCTGCAGCAGTCGGGATGTATCGGTTTAGCTCTTTCTGCAAGTTTG
ACTCACGGTGTCCAGGCATTACCATTTGTTGTTCCTTCAATTGCTTGGCA
ACATCCCTAGCAGACGAACCAGAAACCTCAATCCAAGTTTTAGAGAACAA
TGCACATGCTGACAGCATAAACACTAGATAGAACAAAGCATGGAAAGGAT
TGGCTGCCATATCAGCTAAACTGGACGGTGCAGTGATGTAGTATGCAATA
CCACCAACAGGAATTGAATGACCACCTCCATATTCAGATTCCTTCCATTT
TCCCAACAGATTTACAATGAAGTTTCCACTGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005576	extracellular region
GO:0016020	membrane

Vocabulary: **Molecular Function**
Term	Definition
GO:0030414	peptidase inhibitor activity
GO:0015450	protein-transporting ATPase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR008197	WAP_dom
IPR002208	SecY/SEC61-alpha
IPR023201	SecY_dom_sf

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