AM162132

EST Overview


Name	AM162132
Unique Name	AM162132
Type	EST
Organism	Pisum sativum (pea)
Sequence length	414

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of AM162132 vs. TrEMBL
Match: O24096_MEDTR (Cycloartenol synthase (Fragment) OS=Medicago truncatula GN=MtN18 PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 7.029e-71
Identity = 127/137 (92.70%), Postives = 130/137 (94.89%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFSMHDQGWQVSDCTAEGLKAALLLSKMPS LVGEKLE+ER  DAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREY ECTGSAMQAL LFTKLHP HR KEIHHCL+KAI+YIENTQNPDGSW
Sbjct:  179 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSDLVGEKLESERLYDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYAECTGSAMQALYLFTKLHPTHRAKEIHHCLSKAINYIENTQNPDGSW 315

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q764T8_GLYGL (Lupeol synthase OS=Glycyrrhiza glabra GN=GgLUS1 PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 8.322e-64
Identity = 116/137 (84.67%), Postives = 121/137 (88.32%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFSMHD GWQVSDCTAEGLKAALLLS+MPS LVG K+ETERF DAVNVILSLQSSNGGFPAWEPQ AY WLEKFNPTEFFEDT+IEREYVECTGSAMQ L LF K  P HR+KEI  C+AKAI YIEN QNPDGSW
Sbjct:  469 WTFSMHDHGWQVSDCTAEGLKAALLLSEMPSELVGGKMETERFYDAVNVILSLQSSNGGFPAWEPQKAYRWLEKFNPTEFFEDTMIEREYVECTGSAMQGLALFRKQFPQHRSKEIDRCIAKAIRYIENMQNPDGSW 605

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q2WGL7_LOTJA (Lupeol synthase OS=Lotus japonicus GN=OSC3 PE=2 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 7.045e-63
Identity = 114/137 (83.21%), Postives = 121/137 (88.32%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFSMHD GWQVSDCTAEGLK ALLLS+M   LVG K+ETE+F DAVNVILSLQSSNGGFPAWEPQ AY WLEKFNPTEFFE+TLIEREYVECTGSAMQAL LF KL+P HR KEI  C++KAI YIENTQNPDGSW
Sbjct:  469 WTFSMHDHGWQVSDCTAEGLKVALLLSEMSDDLVGAKMETEQFYDAVNVILSLQSSNGGFPAWEPQRAYQWLEKFNPTEFFEETLIEREYVECTGSAMQALALFRKLYPKHRRKEIDRCISKAIRYIENTQNPDGSW 605

[Close]

BLAST of AM162132 vs. TrEMBL
Match: D7T0K9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_65.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032217001 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.121e-59
Identity = 107/137 (78.10%), Postives = 116/137 (84.67%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCT EGLK ALL S+M   LVGEK+E ERF DAVNVILSLQSSNGGFPAWEPQ A+ WLEKFNPTEFFED LIEREYVECT SA+Q L+LF KLHP HR KEI  C+++AIHYIE+TQNPDGSW
Sbjct:  517 WTFSTQDHGWQVSDCTGEGLKVALLFSQMSPDLVGEKMEKERFYDAVNVILSLQSSNGGFPAWEPQRAFRWLEKFNPTEFFEDVLIEREYVECTSSAIQGLILFIKLHPEHRRKEIESCISRAIHYIEDTQNPDGSW 653

[Close]

BLAST of AM162132 vs. TrEMBL
Match: A5ATS7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020304 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.121e-59
Identity = 107/137 (78.10%), Postives = 116/137 (84.67%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCT EGLK ALL S+M   LVGEK+E ERF DAVNVILSLQSSNGGFPAWEPQ A+ WLEKFNPTEFFED LIEREYVECT SA+Q L+LF KLHP HR KEI  C+++AIHYIE+TQNPDGSW
Sbjct:  470 WTFSTQDHGWQVSDCTGEGLKVALLFSQMSPDLVGEKMEKERFYDAVNVILSLQSSNGGFPAWEPQRAFRWLEKFNPTEFFEDVLIEREYVECTSSAIQGLILFIKLHPEHRRKEIESCISRAIHYIEDTQNPDGSW 606

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q9SLW1_TAROF (Lupeol synthase OS=Taraxacum officinale GN=TRW PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 1.520e-57
Identity = 103/137 (75.18%), Postives = 115/137 (83.94%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS+ D GWQVSDCTAEGLK +LL S+M   LVGEK+ETE   DAVNVILSLQS NGGFPAWEPQ AY+WLEKFNPTEFFED LIEREYVECT SA+Q L LF KLHP HRTKEI HC+++A+ Y+E+TQ  DGSW
Sbjct:  472 WTFSIQDHGWQVSDCTAEGLKVSLLYSQMNPKLVGEKVETEHLYDAVNVILSLQSENGGFPAWEPQRAYAWLEKFNPTEFFEDVLIEREYVECTSSAIQGLTLFKKLHPGHRTKEIEHCISRAVKYVEDTQESDGSW 608

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q9SLW3_OLEEU (Lupeol synthase OS=Olea europaea GN=OEW PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.089e-55
Identity = 103/137 (75.18%), Postives = 114/137 (83.21%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFSM D GWQVSDCTAEGLKAALL S+MP  LVG ++ET    DAVNVIL+LQS++GGFPAWEPQ AY WLEK NPTEFFED LIER+YVECT SA+QAL LF +LHP HR KEI  C++KAI YIE TQNPDGSW
Sbjct:  470 WTFSMQDHGWQVSDCTAEGLKAALLFSQMPIELVGAEIETGHLYDAVNVILTLQSASGGFPAWEPQKAYRWLEKLNPTEFFEDVLIERDYVECTSSAVQALKLFKQLHPGHRRKEIASCISKAIQYIEATQNPDGSW 606

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q8W3Z2_9ROSI (Lupeol synthase OS=Betula platyphylla GN=OSCBPW PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 5.978e-54
Identity = 100/137 (72.99%), Postives = 108/137 (78.83%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFSM D GWQVSDCTAEGLK A+L S+MP  LVGEK+E ER  DAVNVILSLQSSNGGFPAWEPQ AY WLEKFNPTEFFEDTLIEREYVECT  A+  L LF K +P HR  EI   + + I YIE+ Q PDGSW
Sbjct:  469 WTFSMQDHGWQVSDCTAEGLKVAILFSQMPPDLVGEKIEKERLYDAVNVILSLQSSNGGFPAWEPQRAYGWLEKFNPTEFFEDTLIEREYVECTSPAVHGLALFRKFYPRHRGTEIDSSIYRGIQYIEDVQEPDGSW 605

[Close]

BLAST of AM162132 vs. TrEMBL
Match: Q2PPC7_9CARY (Beta-amyrin synthase (Fragment) OS=Gypsophila paniculata GN=bAS PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 8.933e-50
Identity = 92/137 (67.15%), Postives = 108/137 (78.83%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTAEGLK  L+LS MP  +VGEK++ ER  D+VN++LSLQS NGG PAWEP  A +WLE  NPTEFF D +IE EYVECTGSA+QALVLF KLHP HR KEI + ++KA  Y+E+TQ P+GSW
Sbjct:  307 WTFSDQDHGWQVSDCTAEGLKCCLVLSTMPPEIVGEKMDPERLYDSVNILLSLQSENGGLPAWEPAGAQAWLELLNPTEFFADIVIEHEYVECTGSAIQALVLFKKLHPGHRKKEIENFISKASKYLEDTQYPNGSW 443

[Close]

BLAST of AM162132 vs. TrEMBL
Match: B9SMW8_RICCO (Cycloartenol synthase, putative OS=Ricinus communis GN=RCOM_0472110 PE=4 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.290e-48
Identity = 91/137 (66.42%), Postives = 103/137 (75.18%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGWQVSDCTAE LK  LL S MP  +VGEK+E E+  D+VNVILSLQS NGGF AWEP  A SW+E  NP EF ED ++E EYVECT SA+QALVLF KL+P HR KEI +C+  A  +IEN Q PDGSW
Sbjct:  471 WTFSDKDQGWQVSDCTAESLKCCLLFSMMPPEIVGEKMEPEKVYDSVNVILSLQSQNGGFTAWEPARAGSWMEWLNPVEFMEDLVVEHEYVECTSSAIQALVLFKKLYPLHRNKEIENCIINAAQFIENIQEPDGSW 607

[Close]

BLAST of AM162132 vs. SwissProt
Match: LUP1_ARATH (Lupeol synthase 1 OS=Arabidopsis thaliana GN=LUP1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 9.495e-46
Identity = 82/137 (59.85%), Postives = 103/137 (75.18%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTAE LK  LLLS M + +VG+K++ E+  D+VN++LSLQS NGG  AWEP  AY WLE  NPTEF  +T++ERE+VECT S +QAL LF KL+P HR KEI+  + KA+ +I++ Q PDGSW
Sbjct:  470 WTFSDRDHGWQVSDCTAEALKCCLLLSMMSADIVGQKIDDEQLYDSVNLLLSLQSGNGGVNAWEPSRAYKWLELLNPTEFMANTMVEREFVECTSSVIQALDLFRKLYPDHRKKEINRSIEKAVQFIQDNQTPDGSW 606

[Close]

BLAST of AM162132 vs. SwissProt
Match: LUP5_ARATH (Lupeol synthase 5 OS=Arabidopsis thaliana GN=LUP5 PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 7.523e-43
Identity = 77/137 (56.20%), Postives = 98/137 (71.53%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WT S  D GWQVSDCTAE  K  +LLS MP+ + GEK+  E+  D+VN++LSLQS NGGF AWEP  AY W+E  NPT+ F + + EREY ECT + +QALV+F +L+P HRTKEI   + KA+ +IE+ Q  DGSW
Sbjct:  473 WTLSDRDHGWQVSDCTAEAAKCCMLLSTMPTDITGEKINLEQLYDSVNLMLSLQSENGGFTAWEPVRAYKWMELMNPTDLFANAMTEREYTECTSAVLQALVIFNQLYPDHRTKEITKSIEKAVQFIESKQLRDGSW 609

[Close]

BLAST of AM162132 vs. SwissProt
Match: LUP2_ARATH (Lupeol synthase 2 OS=Arabidopsis thaliana GN=LUP2 PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 7.523e-43
Identity = 78/137 (56.93%), Postives = 98/137 (71.53%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WT S  D GWQVSDCTAE LK  +LLS MP+ +VG+K++ E+  D+VN++LSLQ   GG  AWEP  A  WLE  NPT+FF   + EREYVECT + +QALVLF +L+P HRTKEI   + K + +IE+ Q PDGSW
Sbjct:  473 WTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQLYDSVNLLLSLQGEKGGLTAWEPVRAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSIEKGVQFIESKQTPDGSW 609

[Close]

BLAST of AM162132 vs. SwissProt
Match: LUP4_ARATH (Beta-amyrin synthase OS=Arabidopsis thaliana GN=BAS PE=2 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 3.734e-42
Identity = 81/137 (59.12%), Postives = 94/137 (68.61%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTA GLK  LL S +   +VG K + ER +D+VN++LSLQS NGG  AWEP  A  WLE  NPTE F D +IE EY ECT SA+QAL LF +L+P HRT EI   + KA  Y+EN Q  DGSW
Sbjct:  473 WTFSDRDHGWQVSDCTAHGLKCCLLFSMLAPDIVGPKQDPERLHDSVNILLSLQSKNGGMTAWEPAGAPKWLELLNPTEMFSDIVIEHEYSECTSSAIQALSLFKQLYPDHRTTEITAFIKKAAEYLENMQTRDGSW 609

[Close]

BLAST of AM162132 vs. SwissProt
Match: LUP3_ARATH (Camelliol C synthase OS=Arabidopsis thaliana GN=CAMS1 PE=3 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 1.419e-41
Identity = 77/137 (56.20%), Postives = 96/137 (70.07%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQ SDCTAE  K  LLLS +P  +VG K++ E+  +AV ++LSLQS NGG  AWEP     WLE  NPTE F D ++E EY ECT SA+QAL+LF +L+P HRT+EI+  + KA+ YIE+ Q  DGSW
Sbjct:  473 WTFSDRDHGWQASDCTAESFKCCLLLSMIPPDIVGPKMDPEQLYEAVTILLSLQSKNGGVTAWEPARGQEWLELLNPTEVFADIVVEHEYNECTSSAIQALILFKQLYPNHRTEEINTSIKKAVQYIESIQMLDGSW 609

[Close]

BLAST of AM162132 vs. SwissProt
Match: CAS1_ARATH (Cycloartenol synthase OS=Arabidopsis thaliana GN=CAS1 PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 3.161e-41
Identity = 76/137 (55.47%), Postives = 100/137 (72.99%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            W FS  D GW +SDCTAEGLKAALLLSK+P A+VGE ++ +R  +AVNVI+SLQ+++GG   +E   +Y WLE  NP E F D +I+  YVECT +A+QAL+ F KL+P HR KE+  C+ KA+ +IE+ Q  DGSW
Sbjct:  470 WPFSTADHGWPISDCTAEGLKAALLLSKVPKAIVGEPIDAKRLYEAVNVIISLQNADGGLATYELTRSYPWLELINPAETFGDIVIDYPYVECTSAAIQALISFRKLYPGHRKKEVDECIEKAVKFIESIQAADGSW 606

[Close]

BLAST of AM162132 vs. SwissProt
Match: PEN6_ARATH (Pentacyclic triterpene synthase 6 OS=Arabidopsis thaliana GN=PEN6 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.569e-40
Identity = 75/137 (54.74%), Postives = 92/137 (67.15%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE L+  L+   MPS L+GEK++ ER  DAVN++L  QS NGG   WE     +WLE  +P EF EDT++E EYVECTGSA+ AL  F K  P HR +E+   +  A+ YIE+ Q PDGSW
Sbjct:  479 WTFSDQDQGWPVSDCTAESLECCLVFGSMPSELIGEKMDVERLYDAVNLLLYFQSKNGGITVWEAARGRTWLEWLSPVEFMEDTIVEHEYVECTGSAIVALARFLKEFPEHRREEVEKFIKNAVKYIESFQMPDGSW 615

[Close]

BLAST of AM162132 vs. SwissProt
Match: PEN1_ARATH (Arabidiol synthase OS=Arabidopsis thaliana GN=PEN1 PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.049e-40
Identity = 76/137 (55.47%), Postives = 94/137 (68.61%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE LK  LL  +MPS  VG+K++ E+  DAV+ +L LQS NGG  AWEP +  +WLE F+P EF +DT+IE EYVECTGSA+ AL  F+K  P  R KE+   +   + YIE+ Q  DGSW
Sbjct:  478 WTFSDKDQGWPVSDCTAESLKCCLLFERMPSEFVGQKMDVEKLFDAVDFLLYLQSDNGGITAWEPADGKTWLEWFSPVEFVQDTVIEHEYVECTGSAIVALTQFSKQFPEFRKKEVERFITNGVKYIEDLQMKDGSW 614

[Close]

BLAST of AM162132 vs. SwissProt
Match: PEN4_ARATH (Thalianol synthase OS=Arabidopsis thaliana GN=THAS1 PE=2 SV=2)

HSP 1 Score: 157.532 bits (397), Expect = 1.918e-38
Identity = 72/137 (52.55%), Postives = 91/137 (66.42%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE L+  L    MPS L+G+K++ E+  DAV+ +L LQS NGG  AW+P    +WLE  +P EF EDT++E EYVECTGSA+ AL  F K  P ++  E+   + KA  YIE+ Q  DGSW
Sbjct:  478 WTFSDQDQGWPVSDCTAESLECCLFFESMPSELIGKKMDVEKLYDAVDYLLYLQSDNGGIAAWQPVEGKAWLEWLSPVEFLEDTIVEYEYVECTGSAIAALTQFNKQFPGYKNVEVKRFITKAAKYIEDMQTVDGSW 614

[Close]

BLAST of AM162132 vs. SwissProt
Match: PEN7_ARATH (Putative pentacyclic triterpene synthase 7 OS=Arabidopsis thaliana GN=PEN7 PE=5 SV=2)

HSP 1 Score: 155.992 bits (393), Expect = 5.580e-38
Identity = 73/137 (53.28%), Postives = 90/137 (65.69%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            W FS  DQGW  SDCT+E L+  L+   MPS  + EK++ ER  DAVN++L LQS NGG   WE  +   WLE  +P EF E+T++E EYVECTGSA+  L  F K  P HRTKEI   +AKA+ YIE+ Q  DGSW
Sbjct:  339 WGFSDKDQGWPASDCTSESLECCLIFESMPSNFIDEKMDVERLYDAVNMLLYLQSENGGKAVWERASGKKWLEWLSPIEFMEETILEHEYVECTGSAVVVLTRFMKQFPRHRTKEIETFIAKAVKYIESLQMADGSW 475

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78970.1 (| Symbols: LUP1, ATLUP1 | lupeol synthase 1 | chr1:29703414-29707715 FORWARD LENGTH=757)

HSP 1 Score: 181.8 bits (460), Expect = 1.106e-46
Identity = 82/137 (59.85%), Postives = 103/137 (75.18%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTAE LK  LLLS M + +VG+K++ E+  D+VN++LSLQS NGG  AWEP  AY WLE  NPTEF  +T++ERE+VECT S +QAL LF KL+P HR KEI+  + KA+ +I++ Q PDGSW
Sbjct:  470 WTFSDRDHGWQVSDCTAEALKCCLLLSMMSADIVGQKIDDEQLYDSVNLLLSLQSGNGGVNAWEPSRAYKWLELLNPTEFMANTMVEREFVECTSSVIQALDLFRKLYPDHRKKEINRSIEKAVQFIQDNQTPDGSW 606

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78970.2 (| Symbols: LUP1, ATLUP1 | lupeol synthase 1 | chr1:29703414-29707715 FORWARD LENGTH=757)

HSP 1 Score: 181.8 bits (460), Expect = 1.106e-46
Identity = 82/137 (59.85%), Postives = 103/137 (75.18%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTAE LK  LLLS M + +VG+K++ E+  D+VN++LSLQS NGG  AWEP  AY WLE  NPTEF  +T++ERE+VECT S +QAL LF KL+P HR KEI+  + KA+ +I++ Q PDGSW
Sbjct:  470 WTFSDRDHGWQVSDCTAEALKCCLLLSMMSADIVGQKIDDEQLYDSVNLLLSLQSGNGGVNAWEPSRAYKWLELLNPTEFMANTMVEREFVECTSSVIQALDLFRKLYPDHRKKEINRSIEKAVQFIQDNQTPDGSW 606

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G66960.1 (| Symbols: | Terpenoid cyclases family protein | chr1:24985155-24989664 REVERSE LENGTH=763)

HSP 1 Score: 172.17 bits (435), Expect = 8.760e-44
Identity = 77/137 (56.20%), Postives = 98/137 (71.53%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WT S  D GWQVSDCTAE  K  +LLS MP+ + GEK+  E+  D+VN++LSLQS NGGF AWEP  AY W+E  NPT+ F + + EREY ECT + +QALV+F +L+P HRTKEI   + KA+ +IE+ Q  DGSW
Sbjct:  473 WTLSDRDHGWQVSDCTAEAAKCCMLLSTMPTDITGEKINLEQLYDSVNLMLSLQSENGGFTAWEPVRAYKWMELMNPTDLFANAMTEREYTECTSAVLQALVIFNQLYPDHRTKEITKSIEKAVQFIESKQLRDGSW 609

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78960.1 (| Symbols: ATLUP2, LUP2 | lupeol synthase 2 | chr1:29696722-29701024 FORWARD LENGTH=763)

HSP 1 Score: 172.17 bits (435), Expect = 8.760e-44
Identity = 78/137 (56.93%), Postives = 98/137 (71.53%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WT S  D GWQVSDCTAE LK  +LLS MP+ +VG+K++ E+  D+VN++LSLQ   GG  AWEP  A  WLE  NPT+FF   + EREYVECT + +QALVLF +L+P HRTKEI   + K + +IE+ Q PDGSW
Sbjct:  473 WTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQLYDSVNLLLSLQGEKGGLTAWEPVRAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSIEKGVQFIESKQTPDGSW 609

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78950.1 (| Symbols: | Terpenoid cyclases family protein | chr1:29684558-29688673 REVERSE LENGTH=759)

HSP 1 Score: 169.859 bits (429), Expect = 4.348e-43
Identity = 81/137 (59.12%), Postives = 94/137 (68.61%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQVSDCTA GLK  LL S +   +VG K + ER +D+VN++LSLQS NGG  AWEP  A  WLE  NPTE F D +IE EY ECT SA+QAL LF +L+P HRT EI   + KA  Y+EN Q  DGSW
Sbjct:  473 WTFSDRDHGWQVSDCTAHGLKCCLLFSMLAPDIVGPKQDPERLHDSVNILLSLQSKNGGMTAWEPAGAPKWLELLNPTEMFSDIVIEHEYSECTSSAIQALSLFKQLYPDHRTTEITAFIKKAAEYLENMQTRDGSW 609

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78955.1 (| Symbols: CAMS1 | camelliol C synthase 1 | chr1:29689153-29694255 REVERSE LENGTH=769)

HSP 1 Score: 167.933 bits (424), Expect = 1.652e-42
Identity = 77/137 (56.20%), Postives = 96/137 (70.07%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  D GWQ SDCTAE  K  LLLS +P  +VG K++ E+  +AV ++LSLQS NGG  AWEP     WLE  NPTE F D ++E EY ECT SA+QAL+LF +L+P HRT+EI+  + KA+ YIE+ Q  DGSW
Sbjct:  473 WTFSDRDHGWQASDCTAESFKCCLLLSMIPPDIVGPKMDPEQLYEAVTILLSLQSKNGGVTAWEPARGQEWLELLNPTEVFADIVVEHEYNECTSSAIQALILFKQLYPNHRTEEINTSIKKAVQYIESIQMLDGSW 609

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT2G07050.1 (| Symbols: CAS1 | cycloartenol synthase 1 | chr2:2924629-2930295 FORWARD LENGTH=759)

HSP 1 Score: 166.777 bits (421), Expect = 3.680e-42
Identity = 76/137 (55.47%), Postives = 100/137 (72.99%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            W FS  D GW +SDCTAEGLKAALLLSK+P A+VGE ++ +R  +AVNVI+SLQ+++GG   +E   +Y WLE  NP E F D +I+  YVECT +A+QAL+ F KL+P HR KE+  C+ KA+ +IE+ Q  DGSW
Sbjct:  470 WPFSTADHGWPISDCTAEGLKAALLLSKVPKAIVGEPIDAKRLYEAVNVIISLQNADGGLATYELTRSYPWLELINPAETFGDIVIDYPYVECTSAAIQALISFRKLYPGHRKKEVDECIEKAVKFIESIQAADGSW 606

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT1G78500.1 (| Symbols: | Terpenoid cyclases family protein | chr1:29531646-29535177 FORWARD LENGTH=767)

HSP 1 Score: 164.466 bits (415), Expect = 1.827e-41
Identity = 75/137 (54.74%), Postives = 92/137 (67.15%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE L+  L+   MPS L+GEK++ ER  DAVN++L  QS NGG   WE     +WLE  +P EF EDT++E EYVECTGSA+ AL  F K  P HR +E+   +  A+ YIE+ Q PDGSW
Sbjct:  479 WTFSDQDQGWPVSDCTAESLECCLVFGSMPSELIGEKMDVERLYDAVNLLLYFQSKNGGITVWEAARGRTWLEWLSPVEFMEDTIVEHEYVECTGSAIVALARFLKEFPEHRREEVEKFIKNAVKYIESFQMPDGSW 615

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT4G15340.1 (| Symbols: ATPEN1, 04C11, PEN1 | pentacyclic triterpene synthase 1 | chr4:8754670-8760589 REVERSE LENGTH=766)

HSP 1 Score: 164.081 bits (414), Expect = 2.386e-41
Identity = 76/137 (55.47%), Postives = 94/137 (68.61%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE LK  LL  +MPS  VG+K++ E+  DAV+ +L LQS NGG  AWEP +  +WLE F+P EF +DT+IE EYVECTGSA+ AL  F+K  P  R KE+   +   + YIE+ Q  DGSW
Sbjct:  478 WTFSDKDQGWPVSDCTAESLKCCLLFERMPSEFVGQKMDVEKLFDAVDFLLYLQSDNGGITAWEPADGKTWLEWFSPVEFVQDTVIEHEYVECTGSAIVALTQFSKQFPEFRKKEVERFITNGVKYIEDLQMKDGSW 614

[Close]

BLAST of AM162132 vs. TAIR peptide
Match: AT5G48010.2 (| Symbols: THAS, THAS1 | thalianol synthase 1 | chr5:19457001-19461538 FORWARD LENGTH=766)

HSP 1 Score: 157.532 bits (397), Expect = 2.233e-39
Identity = 72/137 (52.55%), Postives = 91/137 (66.42%), Query Frame = -3

Query:    2 WTFSMHDQGWQVSDCTAEGLKAALLLSKMPSALVGEKLETERFNDAVNVILSLQSSNGGFPAWEPQNAYSWLEKFNPTEFFEDTLIEREYVECTGSAMQALVLFTKLHPYHRTKEIHHCLAKAIHYIENTQNPDGSW 412
            WTFS  DQGW VSDCTAE L+  L    MPS L+G+K++ E+  DAV+ +L LQS NGG  AW+P    +WLE  +P EF EDT++E EYVECTGSA+ AL  F K  P ++  E+   + KA  YIE+ Q  DGSW
Sbjct:  478 WTFSDQDQGWPVSDCTAESLECCLFFESMPSELIGKKMDVEKLYDAVDYLLYLQSDNGGIAAWQPVEGKAWLEWLSPVEFLEDTIVEYEYVECTGSAIAALTQFNKQFPGYKNVEVKRFITKAAKYIEDMQTVDGSW 614

The following BLAST results are available for this feature:

BLAST of AM162132 vs. TrEMBL
BLAST of AM162132 vs. SwissProt
BLAST of AM162132 vs. TAIR peptide

BLAST of AM162132 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O24096_MEDTR	7.029e-71	92.70	Cycloartenol synthase (Fragment) OS=Medicago trunc...	[more]
Q764T8_GLYGL	8.322e-64	84.67	Lupeol synthase OS=Glycyrrhiza glabra GN=GgLUS1 PE...	[more]
Q2WGL7_LOTJA	7.045e-63	83.21	Lupeol synthase OS=Lotus japonicus GN=OSC3 PE=2 SV...	[more]
D7T0K9_VITVI	2.121e-59	78.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5ATS7_VITVI	2.121e-59	78.10	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q9SLW1_TAROF	1.520e-57	75.18	Lupeol synthase OS=Taraxacum officinale GN=TRW PE=...	[more]
Q9SLW3_OLEEU	1.089e-55	75.18	Lupeol synthase OS=Olea europaea GN=OEW PE=2 SV=1	[more]
Q8W3Z2_9ROSI	5.978e-54	72.99	Lupeol synthase OS=Betula platyphylla GN=OSCBPW PE...	[more]
Q2PPC7_9CARY	8.933e-50	67.15	Beta-amyrin synthase (Fragment) OS=Gypsophila pani...	[more]
B9SMW8_RICCO	1.290e-48	66.42	Cycloartenol synthase, putative OS=Ricinus communi...	[more]

BLAST of AM162132 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LUP1_ARATH	9.495e-46	59.85	Lupeol synthase 1 OS=Arabidopsis thaliana GN=LUP1 ...	[more]
LUP5_ARATH	7.523e-43	56.20	Lupeol synthase 5 OS=Arabidopsis thaliana GN=LUP5 ...	[more]
LUP2_ARATH	7.523e-43	56.93	Lupeol synthase 2 OS=Arabidopsis thaliana GN=LUP2 ...	[more]
LUP4_ARATH	3.734e-42	59.12	Beta-amyrin synthase OS=Arabidopsis thaliana GN=BA...	[more]
LUP3_ARATH	1.419e-41	56.20	Camelliol C synthase OS=Arabidopsis thaliana GN=CA...	[more]
CAS1_ARATH	3.161e-41	55.47	Cycloartenol synthase OS=Arabidopsis thaliana GN=C...	[more]
PEN6_ARATH	1.569e-40	54.74	Pentacyclic triterpene synthase 6 OS=Arabidopsis t...	[more]
PEN1_ARATH	2.049e-40	55.47	Arabidiol synthase OS=Arabidopsis thaliana GN=PEN1...	[more]
PEN4_ARATH	1.918e-38	52.55	Thalianol synthase OS=Arabidopsis thaliana GN=THAS...	[more]
PEN7_ARATH	5.580e-38	53.28	Putative pentacyclic triterpene synthase 7 OS=Arab...	[more]

BLAST of AM162132 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G78970.1	1.106e-46	59.85	\| Symbols: LUP1, ATLUP1 \| lupeol synthase 1 \| chr1...	[more]
AT1G78970.2	1.106e-46	59.85	\| Symbols: LUP1, ATLUP1 \| lupeol synthase 1 \| chr1...	[more]
AT1G66960.1	8.760e-44	56.20	\| Symbols: \| Terpenoid cyclases family protein \| ...	[more]
AT1G78960.1	8.760e-44	56.93	\| Symbols: ATLUP2, LUP2 \| lupeol synthase 2 \| chr1...	[more]
AT1G78950.1	4.348e-43	59.12	\| Symbols: \| Terpenoid cyclases family protein \| ...	[more]
AT1G78955.1	1.652e-42	56.20	\| Symbols: CAMS1 \| camelliol C synthase 1 \| chr1:2...	[more]
AT2G07050.1	3.680e-42	55.47	\| Symbols: CAS1 \| cycloartenol synthase 1 \| chr2:2...	[more]
AT1G78500.1	1.827e-41	54.74	\| Symbols: \| Terpenoid cyclases family protein \| ...	[more]
AT4G15340.1	2.386e-41	55.47	\| Symbols: ATPEN1, 04C11, PEN1 \| pentacyclic trite...	[more]
AT5G48010.2	2.233e-39	52.55	\| Symbols: THAS, THAS1 \| thalianol synthase 1 \| ch...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008930	Terpenoid cylases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 1..137 score: 4.2
None	No IPR available	GENE3D	1.50.10.20	no description	coord: 1..137 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162132 ID=AM162132; Name=AM162132; organism=Pisum sativum; type=EST; length=414bp
ACCAGGAGCCATCAGGATTTTGTGTGTTTTCAATGTAATGAATTGCTTTG
GCAAGGCAGTGATGTATTTCCTTTGTTCTATGATACGGGTGTAGCTTTGT
GAAGAGTACCAGGGCTTGCATTGCAGAACCAGTGCACTCAACATACTCCC
TCTCAATCAGTGTGTCTTCAAAGAATTCAGTTGGATTGAATTTCTCTAGC
CAACTGTAAGCATTTTGAGGCTCCCAAGCAGGGAACCCACCATTACTGCT
CTGTAGAGAGAGGATGACATTAACAGCATCATTGAATCGCTCTGTTTCTA
ATTTTTCTCCAACAAGAGCACTTGGCATTTTTGACAGTAACAGTGCAGCC
TTGAGTCCTTCTGCGGTGCAGTCCGAGACCTGCCAGCCTTGGTCATGCAT
TGAGAATGTCCACG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR008930	Terpenoid_cyclase/PrenylTrfase

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