AM162147

EST Overview


Name	AM162147
Unique Name	AM162147
Type	EST
Organism	Pisum sativum (pea)
Sequence length	269

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162147 vs. TrEMBL
Match: B9HJD7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_766163 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 7.534e-33
Identity = 75/91 (82.42%), Postives = 84/91 (92.31%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            KES TE+SLDGS+ISD+E GE  +T EKLKSAL++ERKAL  LYAELEEER+ASA+AANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:   95 KESATEESLDGSIISDVEAGEGVLTTEKLKSALRAERKALSALYAELEEERSASAVAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 185

[Close]

BLAST of AM162147 vs. TrEMBL
Match: B9SP67_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0629030 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.863e-32
Identity = 75/91 (82.42%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +ES  E+SLDGSVISDIE G+  +T EKLKSAL+SERKAL  LYAELEEER+ASA+AANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:  620 RESNAEESLDGSVISDIEAGDGVLTVEKLKSALRSERKALNALYAELEEERSASAVAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 710

[Close]

BLAST of AM162147 vs. TrEMBL
Match: D7T8P6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011703001 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 6.378e-32
Identity = 75/91 (82.42%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGEV--TFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +ESGTE+SLDGSVIS+ E G+V  T EKLKSAL+SERKA   LYAELEEER+ASA+AANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:  543 RESGTEESLDGSVISEFEGGDVGLTVEKLKSALRSERKASSVLYAELEEERSASAVAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 633

[Close]

BLAST of AM162147 vs. TrEMBL
Match: A9P7W1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816843 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 9.210e-31
Identity = 71/91 (78.02%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +ESGTE+SLDGSV+S+++ G+  +T E+LK+ALKSERKA   LY ELEEER+ASAIAANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:   52 RESGTEESLDGSVVSEMDSGDPVLTIEQLKTALKSERKAFGALYTELEEERSASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 142

[Close]

BLAST of AM162147 vs. TrEMBL
Match: B9SYW0_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0012640 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.571e-30
Identity = 70/91 (76.92%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +E GTE+SLDGSV+S+ + G+  ++ EKLK+ALK+ERKAL  LY+ELEEER+ASAIAANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:   50 REPGTEESLDGSVVSETDGGDPLLSVEKLKTALKAERKALNALYSELEEERSASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 140

[Close]

BLAST of AM162147 vs. TrEMBL
Match: B9H3A2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_714147 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.571e-30
Identity = 69/91 (75.82%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +ESGTE+SLDGSV+S+++ G+  +T E++K+ALK+ERKA   LY ELEEER+ASAIAANQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:    6 RESGTEESLDGSVVSEMDFGDSVLTVERMKTALKAERKAFGALYTELEEERSASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 96

[Close]

BLAST of AM162147 vs. TrEMBL
Match: D7TSN6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_6.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030780001 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.245e-27
Identity = 68/91 (74.73%), Postives = 79/91 (86.81%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGEVTF--EKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            KES  E+S DGSVIS++E  +  F  E+L +AL++ERKAL+ LYAELEEER+ASAIAANQTMAMI RLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:  272 KESVMENSFDGSVISEMEGSDTVFTIERLTAALEAERKALRALYAELEEERSASAIAANQTMAMITRLQEEKAAMQMEALQYQRMMEEQSE 362

[Close]

BLAST of AM162147 vs. TrEMBL
Match: A5B2M8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000426 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.245e-27
Identity = 68/91 (74.73%), Postives = 79/91 (86.81%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGEVTF--EKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            KES  E+S DGSVIS++E  +  F  E+L +AL++ERKAL+ LYAELEEER+ASAIAANQTMAMI RLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:  573 KESVMENSFDGSVISEMEGSDTVFTIERLTAALEAERKALRALYAELEEERSASAIAANQTMAMITRLQEEKAAMQMEALQYQRMMEEQSE 663

[Close]

BLAST of AM162147 vs. TrEMBL
Match: C5WQS3_SORBI (Putative uncharacterized protein Sb01g040510 OS=Sorghum bicolor GN=Sb01g040510 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.178e-27
Identity = 67/83 (80.72%), Postives = 75/83 (90.36%), Query Frame = 2

Query:   23 SLDGSVISDIECGEV-TFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            SLDGSV S++E GE  T ++LKSAL++ERKAL  LYAELEEERNA+AIA NQTMAMINRLQEEKAAMQMEALQYQRMM+EQSE
Sbjct:  483 SLDGSVASEMEGGEPSTVDQLKSALQAERKALGALYAELEEERNAAAIATNQTMAMINRLQEEKAAMQMEALQYQRMMEEQSE 565

[Close]

BLAST of AM162147 vs. TrEMBL
Match: A2Q381_MEDTR (Putative uncharacterized protein OS=Medicago truncatula GN=MtrDRAFT_AC155282g18v2 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.230e-26
Identity = 61/90 (67.78%), Postives = 78/90 (86.67%), Query Frame = 2

Query:    5 ESGTEDSLDGSVISDIECGE--VTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            ESG E+S+DGSV S++E  +  +T ++LK++LK+E++AL  +Y ELEEER+ASAIA NQTMAMI RLQEEKAAMQMEALQYQRMM+EQ+E
Sbjct:  593 ESGAEESVDGSVASEVEYSDPVLTIDRLKTSLKAEQRALSAIYQELEEERSASAIATNQTMAMITRLQEEKAAMQMEALQYQRMMEEQAE 682

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT5G16720.1 (| Symbols: | Protein of unknown function, DUF593 | chr5:5494309-5496562 FORWARD LENGTH=675)

HSP 1 Score: 104.76 bits (260), Expect = 1.159e-23
Identity = 55/90 (61.11%), Postives = 76/90 (84.44%), Query Frame = 2

Query:    8 SGTEDSLDGSV-ISDIECGEV--TFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +  ED+ DG+V +S+++ G+   T E+L+  +++E++AL+ LYAELEEER+ASAI+ANQTMAMI RLQEEKA +QMEALQYQRMM+EQ+E
Sbjct:  333 AAAEDAGDGNVLVSEMDGGDPLRTIERLRETVRAEQEALRDLYAELEEERSASAISANQTMAMITRLQEEKAKVQMEALQYQRMMEEQAE 422

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G70750.1 (| Symbols: | Protein of unknown function, DUF593 | chr1:26680728-26683147 REVERSE LENGTH=749)

HSP 1 Score: 104.375 bits (259), Expect = 1.514e-23
Identity = 55/78 (70.51%), Postives = 64/78 (82.05%), Query Frame = 2

Query:   41 ISDIEC--GEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            +  IEC  G +T +KLK  L+ ERKAL  LY ELE ERNASA+AA++TMAMINRL EEKAAMQMEALQYQRMM+EQ+E
Sbjct:  401 VDGIECPEGVLTVDKLKFELQEERKALHALYEELEVERNASAVAASETMAMINRLHEEKAAMQMEALQYQRMMEEQAE 478

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT2G30690.1 (| Symbols: | Protein of unknown function, DUF593 | chr2:13076229-13078595 FORWARD LENGTH=760)

HSP 1 Score: 94.3597 bits (233), Expect = 1.567e-20
Identity = 53/78 (67.95%), Postives = 59/78 (75.64%), Query Frame = 2

Query:   35 SVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            S+ SDIE GE   E LK  L+  RK+L+ L  E EEERNASAIA NQ MAMI RLQEEKAA+ MEALQY RMMDEQ+E
Sbjct:  602 SISSDIE-GESLVEVLKQQLEHGRKSLRDLNKEFEEERNASAIATNQAMAMITRLQEEKAALHMEALQYLRMMDEQAE 678

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G74830.1 (| Symbols: | Protein of unknown function, DUF593 | chr1:28113469-28115193 REVERSE LENGTH=542)

HSP 1 Score: 90.5077 bits (223), Expect = 2.263e-19
Identity = 45/70 (64.29%), Postives = 57/70 (81.43%), Query Frame = 2

Query:   59 GEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            GE    +LK  ++ ++K+L  LY EL+EER+ASA+AAN+ MAMI RLQ EKAA+QMEALQYQRMMDEQ+E
Sbjct:  298 GESILNQLKKEVRLDKKSLIDLYMELDEERSASAVAANEAMAMITRLQAEKAAVQMEALQYQRMMDEQAE 367

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G08800.2 (| Symbols: | Protein of unknown function, DUF593 | chr1:2813578-2817078 REVERSE LENGTH=1113)

HSP 1 Score: 89.3521 bits (220), Expect = 5.041e-19
Identity = 49/82 (59.76%), Postives = 62/82 (75.61%), Query Frame = 2

Query:   23 SLDGSVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            SL+G  +++IE GE   ++LK  +  +RK L  LY ELEEER+ASA+A NQ MAMI RLQEEKA+ QMEALQ  RMM+EQ+E
Sbjct:  875 SLEGVSVTEIE-GESEGDRLKRQVDYDRKLLTGLYKELEEERSASAVATNQAMAMITRLQEEKASFQMEALQNLRMMEEQAE 955

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G18990.1 (| Symbols: | Protein of unknown function, DUF593 | chr1:6558778-6560432 REVERSE LENGTH=524)

HSP 1 Score: 89.3521 bits (220), Expect = 5.041e-19
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = 2

Query:   44 SDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            S++  G+   + L   ++ +RK+L  LY EL+EER+ASA+AAN  MAMI RLQ EKAA+QMEALQYQRMMDEQ+E
Sbjct:  292 SEVLDGDSILQHLNRQVRLDRKSLMDLYMELDEERSASAVAANNAMAMITRLQAEKAAVQMEALQYQRMMDEQAE 366

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G08800.1 (| Symbols: | Protein of unknown function, DUF593 | chr1:2813578-2817078 REVERSE LENGTH=1113)

HSP 1 Score: 89.3521 bits (220), Expect = 5.041e-19
Identity = 49/82 (59.76%), Postives = 62/82 (75.61%), Query Frame = 2

Query:   23 SLDGSVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQSE 268
            SL+G  +++IE GE   ++LK  +  +RK L  LY ELEEER+ASA+A NQ MAMI RLQEEKA+ QMEALQ  RMM+EQ+E
Sbjct:  875 SLEGVSVTEIE-GESEGDRLKRQVDYDRKLLTGLYKELEEERSASAVATNQAMAMITRLQEEKASFQMEALQNLRMMEEQAE 955

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT1G04890.1 (| Symbols: | Protein of unknown function, DUF593 | chr1:1381116-1382546 REVERSE LENGTH=411)

HSP 1 Score: 71.2478 bits (173), Expect = 1.421e-13
Identity = 38/88 (43.18%), Postives = 62/88 (70.45%), Query Frame = 2

Query:    2 KESGTEDSLDGSVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQS 265
            ++  +  S + S + ++E   V    L+  LK ER A  T+  EL++ER+A+A AA++ MAMI+RLQ+EKAA++MEA Q+QR+++E+S
Sbjct:   90 QQVNSNSSAEQSFVRNVEKRSV--RDLEELLKEERAARATVCVELDKERSAAASAADEAMAMIHRLQDEKAAIEMEARQFQRLVEERS 175

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT3G54740.2 (| Symbols: | Protein of unknown function, DUF593 | chr3:20262949-20264466 FORWARD LENGTH=438)

HSP 1 Score: 70.0922 bits (170), Expect = 3.165e-13
Identity = 39/85 (45.88%), Postives = 54/85 (63.53%), Query Frame = 2

Query:    8 SGTEDSLDGSVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQ 262
            S    S +  ++ + EC       L  AL S+R+ +K L+ ELEEERNA+A AAN+TM+MI RLQ EKA +QMEA Q++    E+
Sbjct:   55 SSDSSSSNAKILVENECAA-----LLEALSSQRETVKDLHLELEEERNAAASAANETMSMILRLQREKAEIQMEARQFKMFAQEK 134

[Close]

BLAST of AM162147 vs. TAIR peptide
Match: AT3G54740.1 (| Symbols: | Protein of unknown function, DUF593 | chr3:20263093-20264466 FORWARD LENGTH=390)

HSP 1 Score: 70.0922 bits (170), Expect = 3.165e-13
Identity = 39/85 (45.88%), Postives = 54/85 (63.53%), Query Frame = 2

Query:    8 SGTEDSLDGSVISDIECGEVTFEKLKSALKSERKALKTLYAELEEERNASAIAANQTMAMINRLQEEKAAMQMEALQYQRMMDEQ 262
            S    S +  ++ + EC       L  AL S+R+ +K L+ ELEEERNA+A AAN+TM+MI RLQ EKA +QMEA Q++    E+
Sbjct:    7 SSDSSSSNAKILVENECAA-----LLEALSSQRETVKDLHLELEEERNAAASAANETMSMILRLQREKAEIQMEARQFKMFAQEK 86

The following BLAST results are available for this feature:

BLAST of AM162147 vs. TrEMBL
BLAST of AM162147 vs. TAIR peptide

BLAST of AM162147 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HJD7_POPTR	7.534e-33	82.42	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SP67_RICCO	2.863e-32	82.42	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7T8P6_VITVI	6.378e-32	82.42	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9P7W1_POPTR	9.210e-31	78.02	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SYW0_RICCO	4.571e-30	76.92	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9H3A2_POPTR	4.571e-30	75.82	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TSN6_VITVI	1.245e-27	74.73	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B2M8_VITVI	1.245e-27	74.73	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C5WQS3_SORBI	6.178e-27	80.72	Putative uncharacterized protein Sb01g040510 OS=So...	[more]
A2Q381_MEDTR	5.230e-26	67.78	Putative uncharacterized protein OS=Medicago trunc...	[more]

BLAST of AM162147 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G16720.1	1.159e-23	61.11	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G70750.1	1.514e-23	70.51	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT2G30690.1	1.567e-20	67.95	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G74830.1	2.263e-19	64.29	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G08800.2	5.041e-19	59.76	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G18990.1	5.041e-19	60.00	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G08800.1	5.041e-19	59.76	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT1G04890.1	1.421e-13	43.18	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT3G54740.2	3.165e-13	45.88	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]
AT3G54740.1	3.165e-13	45.88	\| Symbols: \| Protein of unknown function, DUF593 ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007656	Protein of unknown function DUF593	PFAM	PF04576	DUF593	coord: 25..89 score: 6.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162147 ID=AM162147; Name=AM162147; organism=Pisum sativum; type=EST; length=269bp
GAAAGAGTCAGGAACAGAAGATTCATTGGACGGAAGTGTAATAAGCGACA
TAGAATGCGGCGAGGTGACATTTGAGAAGTTAAAATCGGCGTTGAAATCG
GAAAGGAAGGCTTTGAAAACATTATACGCAGAACTAGAGGAAGAAAGAAA
TGCGTCTGCCATAGCCGCCAATCAAACAATGGCAATGATTAATAGACTTC
AAGAAGAGAAAGCAGCAATGCAGATGGAAGCTTTGCAGTATCAACGAATG
ATGGACGAACAATCCGAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR007656	GTD-bd

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