CD860471

EST Overview


Name	CD860471
Unique Name	CD860471
Type	EST
Organism	Pisum sativum (pea)
Sequence length	398

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860471 vs. TrEMBL
Match: B9GIU6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_172390 PE=3 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 5.327e-71
Identity = 129/132 (97.73%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWEVFR+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  220 PIYQALEKVNGIAENLSWEVFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 351

[Close]

BLAST of CD860471 vs. TrEMBL
Match: B9TU32_SOLLC (Thiamine biosynthesis protein ThiC variant L1 OS=Solanum lycopersicum GN=thiC PE=3 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 6.957e-71
Identity = 128/132 (96.97%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  286 PIYQALEKVNGIAENLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 417

[Close]

BLAST of CD860471 vs. TrEMBL
Match: B9HP68_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_879767 PE=3 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 6.957e-71
Identity = 129/132 (97.73%), Postives = 130/132 (98.48%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL YHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  233 PIYQALEKVNGIAENLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 364

[Close]

BLAST of CD860471 vs. TrEMBL
Match: A9PJ85_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 6.957e-71
Identity = 129/132 (97.73%), Postives = 130/132 (98.48%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL YHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  277 PIYQALEKVNGIAENLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 408

[Close]

BLAST of CD860471 vs. TrEMBL
Match: Q9AXS1_POASE (Putative thiamine biosythesis protein ThiC OS=Poa secunda PE=2 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.550e-70
Identity = 127/132 (96.21%), Postives = 130/132 (98.48%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            P+YQALEKVNGIAEDLSWEVFR+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL YHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDAND+AQFAELLT GELTRRAWEK
Sbjct:  285 PVYQALEKVNGIAEDLSWEVFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDSAQFAELLTQGELTRRAWEK 416

[Close]

BLAST of CD860471 vs. TrEMBL
Match: B9SHY3_RICCO (Catalytic, putative OS=Ricinus communis GN=RCOM_0611460 PE=3 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.550e-70
Identity = 128/132 (96.97%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAY+HWDDILDICNQYDVALSIGDGLRPGSIYDAND+AQFAELLT GELTRRAWEK
Sbjct:  283 PIYQALEKVNGIAENLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYDHWDDILDICNQYDVALSIGDGLRPGSIYDANDSAQFAELLTQGELTRRAWEK 414

[Close]

BLAST of CD860471 vs. TrEMBL
Match: D7LLB4_ARALY (Thiamine biosynthesis family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_481876 PE=4 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 2.024e-70
Identity = 128/132 (96.97%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV+GIAE+LSWEVFRETLIEQA+QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  285 PIYQALEKVDGIAENLSWEVFRETLIEQADQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 416

[Close]

BLAST of CD860471 vs. TrEMBL
Match: O82392_ARATH (Putative thiamin biosynthesis protein OS=Arabidopsis thaliana GN=At2g29630 PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 4.510e-70
Identity = 127/132 (96.21%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV+GIAE+L+WEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  284 PIYQALEKVDGIAENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 415

[Close]

BLAST of CD860471 vs. TrEMBL
Match: C5WPN8_SORBI (Putative uncharacterized protein Sb01g012050 OS=Sorghum bicolor GN=Sb01g012050 PE=3 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.923e-69
Identity = 125/132 (94.70%), Postives = 129/132 (97.73%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWE+FR+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL YHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDAND+AQFAELLT GELTRRAW K
Sbjct:  282 PIYQALEKVNGIAENLSWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDSAQFAELLTQGELTRRAWAK 413

[Close]

BLAST of CD860471 vs. TrEMBL
Match: C0PEH3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 2.923e-69
Identity = 125/132 (94.70%), Postives = 129/132 (97.73%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKVNGIAE+LSWE+FR+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL YHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDAND+AQFAELLT GELTRRAW K
Sbjct:  285 PIYQALEKVNGIAENLSWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDSAQFAELLTQGELTRRAWAK 416

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_SHELP (Phosphomethylpyrimidine synthase OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=thiC PE=3 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.646e-58
Identity = 103/131 (78.63%), Postives = 120/131 (91.60%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WE+FR+TLIEQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++HKENF YEH+D+I  +C  YDV+LS+GDG+RPGSI DAND AQFAEL T GEL ++AW+
Sbjct:  291 PIYQALEKVNGIAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLYEHFDEICQLCAAYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKKAWQ 421

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_ECOL6 (Phosphomethylpyrimidine synthase OS=Escherichia coli O6 GN=thiC PE=3 SV=2)

HSP 1 Score: 221.861 bits (564), Expect = 8.171e-58
Identity = 104/131 (79.39%), Postives = 120/131 (91.60%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WEVFR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++H+ENF Y+H+ +I +IC  YDV+LS+GDGLRPGSI DAND AQFAEL T GELT+ AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWE 425

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_ECOL5 (Phosphomethylpyrimidine synthase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 8.171e-58
Identity = 104/131 (79.39%), Postives = 120/131 (91.60%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WEVFR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++H+ENF Y+H+ +I +IC  YDV+LS+GDGLRPGSI DAND AQFAEL T GELT+ AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWE 425

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_ECO81 (Phosphomethylpyrimidine synthase OS=Escherichia coli O81 (strain ED1a) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 8.171e-58
Identity = 104/131 (79.39%), Postives = 120/131 (91.60%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WEVFR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++H+ENF Y+H+ +I +IC  YDV+LS+GDGLRPGSI DAND AQFAEL T GELT+ AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWE 425

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_SALAR (Phosphomethylpyrimidine synthase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 1.067e-57
Identity = 104/131 (79.39%), Postives = 119/131 (90.84%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WE FR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++HKENF +EH+ +I +IC  YDV+LS+GDGLRPGSI DAND AQFAEL T GELT+ AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLFEHFREICEICAAYDVSLSLGDGLRPGSIQDANDDAQFAELHTLGELTKIAWE 425

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_COLP3 (Phosphomethylpyrimidine synthase OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 1.067e-57
Identity = 103/131 (78.63%), Postives = 119/131 (90.84%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNG+AEDL+WE+FR+TLIEQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCLA+HKENF Y H++DI +I  QYDV+ S+GDGLRPGS+ DAND AQFAEL T GELT+ AW+
Sbjct:  304 PIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQFAELHTLGELTKIAWK 434

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_CITK8 (Phosphomethylpyrimidine synthase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 1.067e-57
Identity = 104/131 (79.39%), Postives = 119/131 (90.84%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WE FR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++H+ENF YEH+ +I +IC  YDV+LS+GDGLRPGSI DAND AQFAEL T GELT+ AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYEHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWE 425

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_NOVAD (Phosphomethylpyrimidine synthase OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=thiC PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 1.394e-57
Identity = 106/132 (80.30%), Postives = 118/132 (89.39%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV G+AEDL+WEVFR+TLIEQAEQGVDYFTIHAGV L YIPL AKRMTGIVSRGGSI AKWCLA+HKE+F YE++D+I +I   YDVA S+GDGLRPGSIYDAND AQFAEL T GELT+RAWE+
Sbjct:  275 PIYQALEKVGGVAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPYIPLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEITEIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELTKRAWEQ 406

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_SHEHH (Phosphomethylpyrimidine synthase OS=Shewanella halifaxensis (strain HAW-EB4) GN=thiC PE=3 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 1.820e-57
Identity = 103/131 (78.63%), Postives = 118/131 (90.08%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WE FR+TLIEQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++HKENF YEH+ DI +IC  YDV+LS+GDG+RPGSI DAND AQF+EL T GEL + AW+
Sbjct:  291 PIYQALEKVNGIAEDLNWETFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLYEHFRDICEICAAYDVSLSLGDGMRPGSIADANDEAQFSELTTLGELVKIAWQ 421

[Close]

BLAST of CD860471 vs. SwissProt
Match: THIC_SALTI (Phosphomethylpyrimidine synthase OS=Salmonella typhi GN=thiC PE=3 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 1.820e-57
Identity = 103/131 (78.63%), Postives = 119/131 (90.84%), Query Frame = -1

Query:    6 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWE 398
            PIYQALEKVNGIAEDL+WE FR+TL+EQAEQGVDYFTIHAGVLLRY+P+TAKR+TGIVSRGGSI AKWCL++HKENF +EH+ +I +IC+ YDV+LS+GDGLRPGSI DAND AQF+EL T GELT  AWE
Sbjct:  295 PIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHKENFLFEHFREICEICSAYDVSLSLGDGLRPGSIQDANDEAQFSELHTLGELTNIAWE 425

[Close]

BLAST of CD860471 vs. TAIR peptide
Match: AT2G29630.3 (| Symbols: THIC | thiaminC | chr2:12667395-12669569 FORWARD LENGTH=644)

HSP 1 Score: 266.929 bits (681), Expect = 2.398e-72
Identity = 127/132 (96.21%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV+GIAE+L+WEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  284 PIYQALEKVDGIAENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 415

[Close]

BLAST of CD860471 vs. TAIR peptide
Match: AT2G29630.1 (| Symbols: PY, THIC | thiaminC | chr2:12667395-12669569 FORWARD LENGTH=644)

HSP 1 Score: 266.929 bits (681), Expect = 2.398e-72
Identity = 127/132 (96.21%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV+GIAE+L+WEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  284 PIYQALEKVDGIAENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 415

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BLAST of CD860471 vs. TAIR peptide
Match: AT2G29630.2 (| Symbols: THIC | thiaminC | chr2:12667395-12669569 FORWARD LENGTH=644)

HSP 1 Score: 266.929 bits (681), Expect = 2.398e-72
Identity = 127/132 (96.21%), Postives = 131/132 (99.24%), Query Frame = -1

Query:    3 PIYQALEKVNGIAEDLSWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTPGELTRRAWEK 398
            PIYQALEKV+GIAE+L+WEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLT GELTRRAWEK
Sbjct:  284 PIYQALEKVDGIAENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 415

The following BLAST results are available for this feature:

BLAST of CD860471 vs. TrEMBL
BLAST of CD860471 vs. SwissProt
BLAST of CD860471 vs. TAIR peptide

BLAST of CD860471 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GIU6_POPTR	5.327e-71	97.73	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9TU32_SOLLC	6.957e-71	96.97	Thiamine biosynthesis protein ThiC variant L1 OS=S...	[more]
B9HP68_POPTR	6.957e-71	97.73	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PJ85_9ROSI	6.957e-71	97.73	Putative uncharacterized protein OS=Populus tricho...	[more]
Q9AXS1_POASE	1.550e-70	96.21	Putative thiamine biosythesis protein ThiC OS=Poa ...	[more]
B9SHY3_RICCO	1.550e-70	96.97	Catalytic, putative OS=Ricinus communis GN=RCOM_06...	[more]
D7LLB4_ARALY	2.024e-70	96.97	Thiamine biosynthesis family protein OS=Arabidopsi...	[more]
O82392_ARATH	4.510e-70	96.21	Putative thiamin biosynthesis protein OS=Arabidops...	[more]
C5WPN8_SORBI	2.923e-69	94.70	Putative uncharacterized protein Sb01g012050 OS=So...	[more]
C0PEH3_MAIZE	2.923e-69	94.70	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of CD860471 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
THIC_SHELP	1.646e-58	78.63	Phosphomethylpyrimidine synthase OS=Shewanella loi...	[more]
THIC_ECOL6	8.171e-58	79.39	Phosphomethylpyrimidine synthase OS=Escherichia co...	[more]
THIC_ECOL5	8.171e-58	79.39	Phosphomethylpyrimidine synthase OS=Escherichia co...	[more]
THIC_ECO81	8.171e-58	79.39	Phosphomethylpyrimidine synthase OS=Escherichia co...	[more]
THIC_SALAR	1.067e-57	79.39	Phosphomethylpyrimidine synthase OS=Salmonella ari...	[more]
THIC_COLP3	1.067e-57	78.63	Phosphomethylpyrimidine synthase OS=Colwellia psyc...	[more]
THIC_CITK8	1.067e-57	79.39	Phosphomethylpyrimidine synthase OS=Citrobacter ko...	[more]
THIC_NOVAD	1.394e-57	80.30	Phosphomethylpyrimidine synthase OS=Novosphingobiu...	[more]
THIC_SHEHH	1.820e-57	78.63	Phosphomethylpyrimidine synthase OS=Shewanella hal...	[more]
THIC_SALTI	1.820e-57	78.63	Phosphomethylpyrimidine synthase OS=Salmonella typ...	[more]

BLAST of CD860471 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G29630.3	2.398e-72	96.21	\| Symbols: THIC \| thiaminC \| chr2:12667395-1266956...	[more]
AT2G29630.1	2.398e-72	96.21	\| Symbols: PY, THIC \| thiaminC \| chr2:12667395-126...	[more]
AT2G29630.2	2.398e-72	96.21	\| Symbols: THIC \| thiaminC \| chr2:12667395-1266956...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002817	Thiamine biosynthesis protein ThiC	PFAM	PF01964	ThiC	coord: 1..132 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860471 ID=CD860471; Name=CD860471; organism=Pisum sativum; type=EST; length=398bp
TCCTTTTCCCATGCTCTACGAGTGAGTTCACCTGGTGTCAAAAGCTCGGC
AAATTGAGCTGTGTCATTTGCATCATAAATGGATCCAGGTCTTAATCCAT
CACCAATGGACAAAGCCACATCATACTGATTGCATATGTCAAGTATGTCA
TCCCAGTGCTCATAAGCGAAATTCTCTTTGTGATAAGCTAAGCACCACTT
TGCATGAATCGATCCTCCTCTGGAAACTATTCCCGTCATGCGCTTTGCAG
TTAAAGGAATATACCTAAGAAGCACTCCTGCATGAATGGTGAAGTAATCA
ACACCTTGTTCAGCTTGTTCAATCAAGGTTTCTCTAAACACCTCCCAGCT
AAGATCTTCGGCAATTCCGTTAACTTTCTCAAGTGCTTGATAAATAGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0009228	thiamine biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002817	ThiC/BzaA/B

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