EX569994

EST Overview


Name	EX569994
Unique Name	EX569994
Type	EST
Organism	Pisum sativum (pea)
Sequence length	618

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX569994 vs. TrEMBL
Match: B0FPD7_SOLLC (Putative DNA cytosine 5-methyltransferase OS=Solanum lycopersicum GN=DRM5 PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.931e-63
Identity = 119/149 (79.87%), Postives = 136/149 (91.28%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPR 448
            SLGNSFQVDTVAYHLSVLK+MFP+G+NVLSLFSGIGGAEVAL++LG+ L NVVSVEKS+VNR+IVRSWWEQTNQ+GNLID DDVQQL+ DRLEQL+ + G FDL++GGSPCNNLAGSNRV+R GLEG ES+LFYDY RILDLVK +  R
Sbjct:  452 SLGNSFQVDTVAYHLSVLKDMFPNGMNVLSLFSGIGGAEVALYRLGIQLNNVVSVEKSEVNRNIVRSWWEQTNQRGNLIDFDDVQQLNGDRLEQLIDSCGGFDLLIGGSPCNNLAGSNRVSRDGLEGKESSLFYDYVRILDLVKSIMSR 600

[Close]

BLAST of EX569994 vs. TrEMBL
Match: B1GYI2_NICSY (Domains rearranged methyltransferase OS=Nicotiana sylvestris GN=drm PE=2 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 4.302e-63
Identity = 119/149 (79.87%), Postives = 133/149 (89.26%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPR 448
            SLGNSFQVDTVAYHLSVLK +FPDGINVLSLFSGIGG EVAL++LG+ L  VVSVEKS+VNRDIVRSWWEQTNQKGNLI  +DVQQL+ DRLEQL+ +FG FDL++GGSPCNNLAGSNRV+R GLEG ES+LFYDY RILDLVK +  R
Sbjct:  457 SLGNSFQVDTVAYHLSVLKNLFPDGINVLSLFSGIGGGEVALYRLGIPLNTVVSVEKSEVNRDIVRSWWEQTNQKGNLIHFNDVQQLNGDRLEQLIESFGGFDLVIGGSPCNNLAGSNRVSRDGLEGKESSLFYDYVRILDLVKSIMSR 605

[Close]

BLAST of EX569994 vs. TrEMBL
Match: Q76KU6_TOBAC (DNA methyltransferase OS=Nicotiana tabacum GN=NtDRM1 PE=2 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 3.642e-62
Identity = 117/149 (78.52%), Postives = 133/149 (89.26%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPR 448
            SLGNSFQVDTVAYHLSVLK++FP GINVLSLFSGIGG EVAL++LG+ L  VVSVEKS+VNRDIVRSWWEQTNQ+GNLI  +DVQQL+ DRLEQL+ +FG FDL++GGSPCNNLAGSNRV+R GLEG ES+LFYDY RILDLVK +  R
Sbjct:  457 SLGNSFQVDTVAYHLSVLKDLFPGGINVLSLFSGIGGGEVALYRLGIPLNTVVSVEKSEVNRDIVRSWWEQTNQRGNLIHFNDVQQLNGDRLEQLIESFGGFDLVIGGSPCNNLAGSNRVSRDGLEGKESSLFYDYVRILDLVKSIMSR 605

[Close]

BLAST of EX569994 vs. TrEMBL
Match: C4P088_FRAAN (DRM-type cytosine DNA-methyltransferase OS=Fragaria ananassa GN=DRMa PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 3.408e-60
Identity = 111/146 (76.03%), Postives = 132/146 (90.41%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGM 439
            SLGNSFQ+DTVAYHLSVLK+ FP+GIN+LSLFSGIGGAE+ALH+LG+ +KNVVSVE S V+R +VR+WWEQTNQKGNL  + DVQ+L+ADRLE  ++TFG FDL+VGGSPCNNLAGSNR +R GLEG ES+LFYDYFRILD+VKG+
Sbjct:  446 SLGNSFQIDTVAYHLSVLKDRFPNGINLLSLFSGIGGAEIALHRLGIRMKNVVSVEISAVSRTVVRTWWEQTNQKGNLYHLADVQELNADRLEHYINTFGGFDLVVGGSPCNNLAGSNRHHRDGLEGKESSLFYDYFRILDIVKGL 591

[Close]

BLAST of EX569994 vs. TrEMBL
Match: C4P089_FRAAN (DRM-type cytosine DNA-methyltransferase OS=Fragaria ananassa GN=DRMb PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.451e-60
Identity = 111/146 (76.03%), Postives = 132/146 (90.41%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGM 439
            SLGNSFQ+DTVAYHLSVLK+ FP+GIN+LSLFSGIGGAE+ALH+LG+ +KNVVSVE S V+R +VR+WWEQTNQKGNL  + DVQ+L+ADRLE  ++TFG FDL+VGGSPCNNLAGSNR +R GLEG ES+LFYDYFRILD+VKG+
Sbjct:  446 SLGNSFQIDTVAYHLSVLKDRFPNGINLLSLFSGIGGAEIALHRLGIRMKNVVSVEISGVSRTVVRTWWEQTNQKGNLYHLADVQELNADRLEHYINTFGGFDLVVGGSPCNNLAGSNRHHRDGLEGKESSLFYDYFRILDIVKGL 591

[Close]

BLAST of EX569994 vs. TrEMBL
Match: B9GI45_POPTR (DNA methyltransferase OS=Populus trichocarpa GN=DMT905 PE=4 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 9.917e-60
Identity = 114/150 (76.00%), Postives = 131/150 (87.33%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPRF 451
            SLGNSFQVDTVAYHLS LK++FP GINVLSLFSGIGGAEVALH+LG+ LKNVVSVE S VNR I+  WWEQTNQ GNLI ++DVQ L ADRLEQLM+ +G+FDL+VGGSPCNNLAGSNR +R GLEG ES+LF+DY RILD+VK +T R+
Sbjct:  194 SLGNSFQVDTVAYHLSSLKDLFPGGINVLSLFSGIGGAEVALHRLGIRLKNVVSVEISNVNRSIMSCWWEQTNQTGNLIHIEDVQHLTADRLEQLMNMYGSFDLVVGGSPCNNLAGSNRHHRDGLEGKESSLFFDYCRILDVVKNLTSRY 343

[Close]

BLAST of EX569994 vs. TrEMBL
Match: B9S630_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1063380 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.692e-59
Identity = 115/149 (77.18%), Postives = 132/149 (88.59%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPR 448
            SLGNSFQVDTVAYHLSVLK+MFP+GI++LSLFSGIGGAEVALH+LG+ LK VVSVE S+VNR+I+R WWEQTNQ G LID+ DV  L+ADRLEQLMS+FG FDL+VGGSPCNNLAGSNR +R GLEG ES+LF+DY RILDLVK +  R
Sbjct:  537 SLGNSFQVDTVAYHLSVLKDMFPEGISLLSLFSGIGGAEVALHRLGIRLKRVVSVEISEVNRNIMRCWWEQTNQTGTLIDIADVHDLNADRLEQLMSSFGGFDLVVGGSPCNNLAGSNRHHRDGLEGKESSLFFDYCRILDLVKCIMTR 685

[Close]

BLAST of EX569994 vs. TrEMBL
Match: B4XTT0_ELAGV (DRM-type DNA-methyltransferase OS=Elaeis guineensis var. tenera PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 2.209e-59
Identity = 111/144 (77.08%), Postives = 130/144 (90.28%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            SLGNSFQVDTVAYHLSVLK+MFP+GI VLSLFSGIGGAEVALH+LG+ LK VVSVE S VNR+I++SWWEQTNQ G LID+ DVQ+L+ D+LEQ+++TFG FDL++GGSPCNNL+GSNR +R GLEG  S+LFYDYFRILDLVK
Sbjct:  441 SLGNSFQVDTVAYHLSVLKDMFPNGITVLSLFSGIGGAEVALHRLGIHLKTVVSVEISDVNRNILKSWWEQTNQTGELIDLFDVQELNGDKLEQMINTFGGFDLVIGGSPCNNLSGSNRYSRDGLEGKHSSLFYDYFRILDLVK 584

[Close]

BLAST of EX569994 vs. TrEMBL
Match: B9SHC1_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0528970 PE=4 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 6.226e-54
Identity = 102/144 (70.83%), Postives = 125/144 (86.81%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            +LGNSFQVDTVAYHLSVLK +FP GI VLSLFSGIGGAEVALH+LG+ L +VVSVE S+VNR+I RSWWEQTNQKG LI++ DV+++D D +++ +  FG FDL++GGSPCNNLAG+NR NR GLEG +S+LFYDY+RIL LV+
Sbjct:  315 ALGNSFQVDTVAYHLSVLKNLFPYGITVLSLFSGIGGAEVALHRLGIPLNSVVSVEISEVNRNIPRSWWEQTNQKGYLIEIGDVREVDGDSIKRWIKLFGGFDLVIGGSPCNNLAGANRRNRDGLEGEQSSLFYDYYRILKLVR 458

[Close]

BLAST of EX569994 vs. TrEMBL
Match: C5WMN0_SORBI (Putative uncharacterized protein Sb01g049860 OS=Sorghum bicolor GN=Sb01g049860 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 2.616e-52
Identity = 100/146 (68.49%), Postives = 125/146 (85.62%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGM 439
            SLGNSFQVDTVAYHLSVLK+M+P+G+NVLSLFSGIGGAEVALH+LG+ +K V+SVEKS+VNR I+RSWW+QT Q G LI+++DVQ L ++R+E  +  FG FDL++GGSPCNNLAGSNR +R GLEG  S+LF+ Y RIL+ VK +
Sbjct:  593 SLGNSFQVDTVAYHLSVLKDMYPEGMNVLSLFSGIGGAEVALHRLGIRMKTVISVEKSEVNRTILRSWWDQT-QTGTLIEINDVQTLTSERIEAYIRRFGGFDLVIGGSPCNNLAGSNRHHRNGLEGEHSSLFFQYVRILESVKSI 737

[Close]

BLAST of EX569994 vs. SwissProt
Match: DRM2_ARATH (DNA (cytosine-5)-methyltransferase DRM2 OS=Arabidopsis thaliana GN=DRM2 PE=1 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 7.775e-52
Identity = 96/144 (66.67%), Postives = 121/144 (84.03%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            SLGNSFQVDTVAYHLSVLK +FP GINVLSLF+GIGG EVALH+L + +K VVSVE S+VNR+I++ +WEQTNQ G LI+  D+Q L  D +E LM  +G FDL++GGSPCNNLAG NRV+R+GLEG +S+LF++Y RIL++V+
Sbjct:  477 SLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVR 620

[Close]

BLAST of EX569994 vs. SwissProt
Match: DRM1_ARATH (DNA (cytosine-5)-methyltransferase DRM1 OS=Arabidopsis thaliana GN=DRM1 PE=4 SV=2)

HSP 1 Score: 198.749 bits (504), Expect = 1.915e-50
Identity = 96/144 (66.67%), Postives = 119/144 (82.64%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            SLGNSFQVDTVAYHLSVLK +FP+GINVLSLF+GIGG EVALH+L + +  VVSVE S  NR+I+RS+WEQTNQKG L +  DVQ+LD + +E+LM  +G FDL++GGSPCNNLAG NR +R+GL G  S+LF+DY RIL+ V+
Sbjct:  473 SLGNSFQVDTVAYHLSVLKPLFPNGINVLSLFTGIGGGEVALHRLQIKMNVVVSVEISDANRNILRSFWEQTNQKGILREFKDVQKLDDNTIERLMDEYGGFDLVIGGSPCNNLAGGNRHHRVGLGGEHSSLFFDYCRILEAVR 616

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3B_HUMAN (DNA (cytosine-5)-methyltransferase 3B OS=Homo sapiens GN=DNMT3B PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.813e-8
Identity = 36/116 (31.03%), Postives = 63/116 (54.31%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILD 424
            I VLSLF GI    + L +LG+ +   V+ E  + +  +          +GN+  ++DV+ +    +E+    +G FDL++GGSPCN+L+  N   R GL      LF++++ +L+
Sbjct:  575 IRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAV-----GTVKHEGNIKYVNDVRNITKKNIEE----WGPFDLVIGGSPCNDLSNVNPA-RKGLYEGTGRLFFEFYHLLN 680

[Close]

BLAST of EX569994 vs. SwissProt
Match: MTBR_BPRH1 (Modification methylase Rho11sI OS=Bacillus phage rho11s PE=3 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 5.276e-8
Identity = 41/125 (32.80%), Postives = 65/125 (52.00%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPRF 451
            + V+SLFSGIG  E AL  +GV       V  S++++  ++S+    N    L +  DV ++D  +L +       FDL+VGGSPC + + +    R G E +   LF+ Y   +D +K   PR+
Sbjct:    4 LRVMSLFSGIGAFEAALRNIGV---EYELVGFSEIDKYAIKSYCAIHNADEQL-NFGDVSKIDKKKLPE-------FDLLVGGSPCQSFSVAG--YRKGFEDTRGTLFFQY---IDTLKEKQPRY 112

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3B_MOUSE (DNA (cytosine-5)-methyltransferase 3B OS=Mus musculus GN=Dnmt3b PE=1 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 6.890e-8
Identity = 34/119 (28.57%), Postives = 64/119 (53.78%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            I VLSLF GI    + L +LG+ ++  ++ E    +  +          +G +  ++DV+++    +E+    +G FDL++GGSPCN+L+  N   R GL      LF++++ +L+  +
Sbjct:  581 IRVLSLFDGIATGYLVLKELGIKVEKYIASEVCAESIAV-----GTVKHEGQIKYVNDVRKITKKNIEE----WGPFDLVIGGSPCNDLSNVNPA-RKGLYEGTGRLFFEFYHLLNYTR 689

[Close]

BLAST of EX569994 vs. SwissProt
Match: MTBS_BPSPR (Modification methylase SPRI OS=Bacillus phage SPR PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.175e-7
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGMTPRF 451
            + V+SLFSGIG  E AL  +GV       V  S++++  V+S+    N    L +  DV ++D  +L +       FDL+VGGSPC + + +   +R G E +   LF+ Y   ++ +K   P+F
Sbjct:    4 LRVMSLFSGIGAFEAALRNIGV---GYELVGFSEIDKYAVKSFCAIHNVDEQL-NFGDVSKIDKKKLPE-------FDLLVGGSPCQSFSVAG--HRKGFEDTRGTLFFQY---VETLKEKQPKF 112

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3A_RAT (DNA (cytosine-5)-methyltransferase 3A OS=Rattus norvegicus GN=Dnmt3a PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.005e-7
Identity = 36/117 (30.77%), Postives = 63/117 (53.85%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVE--KSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRIL 421
            I VLSLF GI    + L  LG+ +   ++ E  +  +   +VR        +G ++ + DV+ +    +++    +G FDL++GGSPCN+L+  N   R GL      LF++++R+L
Sbjct:  630 IRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVR-------HQGKIMYVGDVRSVTQKHIQE----WGPFDLVIGGSPCNDLSIVNPA-RKGLYEGTGRLFFEFYRLL 734

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3A_MOUSE (DNA (cytosine-5)-methyltransferase 3A OS=Mus musculus GN=Dnmt3a PE=1 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 2.005e-7
Identity = 36/117 (30.77%), Postives = 63/117 (53.85%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVE--KSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRIL 421
            I VLSLF GI    + L  LG+ +   ++ E  +  +   +VR        +G ++ + DV+ +    +++    +G FDL++GGSPCN+L+  N   R GL      LF++++R+L
Sbjct:  630 IRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVR-------HQGKIMYVGDVRSVTQKHIQE----WGPFDLVIGGSPCNDLSIVNPA-RKGLYEGTGRLFFEFYRLL 734

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3A_HUMAN (DNA (cytosine-5)-methyltransferase 3A OS=Homo sapiens GN=DNMT3A PE=1 SV=4)

HSP 1 Score: 55.8398 bits (133), Expect = 2.005e-7
Identity = 36/117 (30.77%), Postives = 63/117 (53.85%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVE--KSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRIL 421
            I VLSLF GI    + L  LG+ +   ++ E  +  +   +VR        +G ++ + DV+ +    +++    +G FDL++GGSPCN+L+  N   R GL      LF++++R+L
Sbjct:  634 IRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVR-------HQGKIMYVGDVRSVTQKHIQE----WGPFDLVIGGSPCNDLSIVNPA-RKGLYEGTGRLFFEFYRLL 738

[Close]

BLAST of EX569994 vs. SwissProt
Match: DNM3A_CHICK (DNA (cytosine-5)-methyltransferase 3A OS=Gallus gallus GN=DNMT3A PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.005e-7
Identity = 36/117 (30.77%), Postives = 63/117 (53.85%), Query Frame = 2

Query:   77 INVLSLFSGIGGAEVALHKLGVLLKNVVSVE--KSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRIL 421
            I VLSLF GI    + L  LG+ +   ++ E  +  +   +VR        +G ++ + DV+ +    +++    +G FDL++GGSPCN+L+  N   R GL      LF++++R+L
Sbjct:  599 IRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVR-------HQGKIMYVGDVRNVTQKHIQE----WGPFDLVIGGSPCNDLSIVNPA-RKGLYEGTGRLFFEFYRLL 703

[Close]

BLAST of EX569994 vs. TAIR peptide
Match: AT5G14620.1 (| Symbols: DRM2, DMT7 | domains rearranged methyltransferase 2 | chr5:4715429-4718578 REVERSE LENGTH=626)

HSP 1 Score: 203.371 bits (516), Expect = 8.075e-53
Identity = 96/144 (66.67%), Postives = 121/144 (84.03%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            SLGNSFQVDTVAYHLSVLK +FP GINVLSLF+GIGG EVALH+L + +K VVSVE S+VNR+I++ +WEQTNQ G LI+  D+Q L  D +E LM  +G FDL++GGSPCNNLAG NRV+R+GLEG +S+LF++Y RIL++V+
Sbjct:  477 SLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVR 620

[Close]

BLAST of EX569994 vs. TAIR peptide
Match: AT5G15380.1 (| Symbols: DRM1 | domains rearranged methylase 1 | chr5:4991347-4994826 FORWARD LENGTH=624)

HSP 1 Score: 198.749 bits (504), Expect = 1.989e-51
Identity = 96/144 (66.67%), Postives = 119/144 (82.64%), Query Frame = 2

Query:    2 SLGNSFQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVK 433
            SLGNSFQVDTVAYHLSVLK +FP+GINVLSLF+GIGG EVALH+L + +  VVSVE S  NR+I+RS+WEQTNQKG L +  DVQ+LD + +E+LM  +G FDL++GGSPCNNLAG NR +R+GL G  S+LF+DY RIL+ V+
Sbjct:  473 SLGNSFQVDTVAYHLSVLKPLFPNGINVLSLFTGIGGGEVALHRLQIKMNVVVSVEISDANRNILRSFWEQTNQKGILREFKDVQKLDDNTIERLMDEYGGFDLVIGGSPCNNLAGGNRHHRVGLGGEHSSLFFDYCRILEAVR 616

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BLAST of EX569994 vs. TAIR peptide
Match: AT3G17310.1 (| Symbols: DRM3, AtDRM3 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr3:5909294-5912838 REVERSE LENGTH=710)

HSP 1 Score: 131.724 bits (330), Expect = 2.986e-31
Identity = 64/141 (45.39%), Postives = 92/141 (65.25%), Query Frame = 2

Query:   17 FQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGM 439
            FQ DT+ YHLSVLK MFP G+ VLSLFSGIGGAE+AL +LG+ LK VVSVE   ++R+I++ WW+ + Q G L+ +++++ L A RLE LM  FG FD ++  +P   L  S  ++       +  LF ++ R+   V+ M
Sbjct:  569 FQTDTLGYHLSVLKSMFPQGLTVLSLFSGIGGAEIALDRLGIHLKGVVSVESCGLSRNILKRWWQTSGQTGELVQIEEIKSLTAKRLETLMQRFGGFDFVICQNPSTPLDLSKEISNSEACEFDYTLFNEFARVTKRVRDM 709

[Close]

BLAST of EX569994 vs. TAIR peptide
Match: AT3G17310.2 (| Symbols: DRM3, AtDRM3 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr3:5909294-5912838 REVERSE LENGTH=710)

HSP 1 Score: 131.724 bits (330), Expect = 2.986e-31
Identity = 64/141 (45.39%), Postives = 92/141 (65.25%), Query Frame = 2

Query:   17 FQVDTVAYHLSVLKEMFPDGINVLSLFSGIGGAEVALHKLGVLLKNVVSVEKSQVNRDIVRSWWEQTNQKGNLIDMDDVQQLDADRLEQLMSTFGAFDLIVGGSPCNNLAGSNRVNRIGLEGSESALFYDYFRILDLVKGM 439
            FQ DT+ YHLSVLK MFP G+ VLSLFSGIGGAE+AL +LG+ LK VVSVE   ++R+I++ WW+ + Q G L+ +++++ L A RLE LM  FG FD ++  +P   L  S  ++       +  LF ++ R+   V+ M
Sbjct:  569 FQTDTLGYHLSVLKSMFPQGLTVLSLFSGIGGAEIALDRLGIHLKGVVSVESCGLSRNILKRWWQTSGQTGELVQIEEIKSLTAKRLETLMQRFGGFDFVICQNPSTPLDLSKEISNSEACEFDYTLFNEFARVTKRVRDM 709

The following BLAST results are available for this feature:

BLAST of EX569994 vs. TrEMBL
BLAST of EX569994 vs. SwissProt
BLAST of EX569994 vs. TAIR peptide

BLAST of EX569994 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B0FPD7_SOLLC	1.931e-63	79.87	Putative DNA cytosine 5-methyltransferase OS=Solan...	[more]
B1GYI2_NICSY	4.302e-63	79.87	Domains rearranged methyltransferase OS=Nicotiana ...	[more]
Q76KU6_TOBAC	3.642e-62	78.52	DNA methyltransferase OS=Nicotiana tabacum GN=NtDR...	[more]
C4P088_FRAAN	3.408e-60	76.03	DRM-type cytosine DNA-methyltransferase OS=Fragari...	[more]
C4P089_FRAAN	4.451e-60	76.03	DRM-type cytosine DNA-methyltransferase OS=Fragari...	[more]
B9GI45_POPTR	9.917e-60	76.00	DNA methyltransferase OS=Populus trichocarpa GN=DM...	[more]
B9S630_RICCO	1.692e-59	77.18	Putative uncharacterized protein OS=Ricinus commun...	[more]
B4XTT0_ELAGV	2.209e-59	77.08	DRM-type DNA-methyltransferase OS=Elaeis guineensi...	[more]
B9SHC1_RICCO	6.226e-54	70.83	Putative uncharacterized protein OS=Ricinus commun...	[more]
C5WMN0_SORBI	2.616e-52	68.49	Putative uncharacterized protein Sb01g049860 OS=So...	[more]

BLAST of EX569994 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DRM2_ARATH	7.775e-52	66.67	DNA (cytosine-5)-methyltransferase DRM2 OS=Arabido...	[more]
DRM1_ARATH	1.915e-50	66.67	DNA (cytosine-5)-methyltransferase DRM1 OS=Arabido...	[more]
DNM3B_HUMAN	1.813e-8	31.03	DNA (cytosine-5)-methyltransferase 3B OS=Homo sapi...	[more]
MTBR_BPRH1	5.276e-8	32.80	Modification methylase Rho11sI OS=Bacillus phage r...	[more]
DNM3B_MOUSE	6.890e-8	28.57	DNA (cytosine-5)-methyltransferase 3B OS=Mus muscu...	[more]
MTBS_BPSPR	1.175e-7	32.80	Modification methylase SPRI OS=Bacillus phage SPR ...	[more]
DNM3A_RAT	2.005e-7	30.77	DNA (cytosine-5)-methyltransferase 3A OS=Rattus no...	[more]
DNM3A_MOUSE	2.005e-7	30.77	DNA (cytosine-5)-methyltransferase 3A OS=Mus muscu...	[more]
DNM3A_HUMAN	2.005e-7	30.77	DNA (cytosine-5)-methyltransferase 3A OS=Homo sapi...	[more]
DNM3A_CHICK	2.005e-7	30.77	DNA (cytosine-5)-methyltransferase 3A OS=Gallus ga...	[more]

BLAST of EX569994 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G14620.1	8.075e-53	66.67	\| Symbols: DRM2, DMT7 \| domains rearranged methylt...	[more]
AT5G15380.1	1.989e-51	66.67	\| Symbols: DRM1 \| domains rearranged methylase 1 \|...	[more]
AT3G17310.1	2.986e-31	45.39	\| Symbols: DRM3, AtDRM3 \| S-adenosyl-L-methionine-...	[more]
AT3G17310.2	2.986e-31	45.39	\| Symbols: DRM3, AtDRM3 \| S-adenosyl-L-methionine-...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001525	C-5 cytosine methyltransferase	PFAM	PF00145	DNA_methylase	coord: 26..141 score: 6.1
None	No IPR available	GENE3D	3.40.50.150	no description	coord: 26..144 score: 1.1
None	No IPR available	PANTHER	PTHR23068	DNA (CYTOSINE-5-)-METHYLTRANSFERASE 3-RELATED	coord: 4..146 score: 8.2
None	No IPR available	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 15..150 score: 2.8
None	No IPR available	SEG	seg	seg	coord: 15..27 score: NAcoord: 38..55 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX569994 ID=EX569994; Name=EX569994; organism=Pisum sativum; type=EST; length=618bp
GTCACTTGGAAATTCTTTCCAGGTTGACACAGTGGCATATCACCTATCTG
TTCTGAAGGAAATGTTTCCTGATGGGATCAATGTTCTCTCTCTCTTTTCT
GGAATTGGTGGTGCAGAGGTAGCTCTCCATAAACTTGGTGTCCTTCTAAA
GAATGTAGTGTCCGTTGAAAAATCTCAAGTGAATAGGGACATTGTTAGAA
GTTGGTGGGAGCAAACTAATCAAAAAGGTAATTTGATTGATATGGATGAT
GTGCAACAACTAGATGCTGATCGTTTGGAGCAGCTGATGAGCACATTTGG
TGCTTTTGATCTCATTGTTGGTGGGAGTCCATGTAATAACCTGGCTGGAA
GCAACAGAGTAAACCGGATTGGACTCGAAGGCTCAGAATCAGCCCTCTTT
TATGATTACTTTCGAATTTTAGACCTGGTGAAAGGTATGACACCAAGATT
TCAATGAAATGGTCTATAGAAGTTTCCTTTTCAAATTATGTATTAATTCC
AAACATGTAGTATCCTCTTATACAACTTCAACAGTATGCAGACACTTTTC
ATCCCATTTTTGTTGTCGAACAAGGAAAGACAACGGTACTAAATAATTTT
TTCCTTAATATTTCTGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006306	DNA methylation

Vocabulary: **INTERPRO**
Term	Definition
IPR001525	C5_MeTfrase

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