EX570178

EST Overview
NameEX570178
Unique NameEX570178
TypeEST
OrganismPisum sativum (pea)
Sequence length437
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of EX570178 vs. TrEMBL
Match: D7T3B0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_73.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034613001 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.205e-24
Identity = 57/82 (69.51%), Postives = 65/82 (79.27%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRDLVTRSTSTAEFVRACIITIMATLLTSLYL 246
            G+TDVLFGDYGFSGKL+RTWFK+VDQLPMNVGD HYDPLFPFGFGL +++V   V RSTS     R  + TIMA +  SLYL
Sbjct:  536 GLTDVLFGDYGFSGKLSRTWFKNVDQLPMNVGDSHYDPLFPFGFGLATKTV---VARSTSAGVGGRPHLFTIMAMVFISLYL 614
[Close]
BLAST of EX570178 vs. TrEMBL
Match: B9RL34_RICCO (Periplasmic beta-glucosidase, putative OS=Ricinus communis GN=RCOM_0944030 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.980e-21
Identity = 46/73 (63.01%), Postives = 59/73 (80.82%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRDLVTRSTSTAEFVRACIITIM 219
            GVTDVLFGDYGF+GKL RTWFK+V+QLPMN+GD HYDPLFPF FG+ ++S   +V RSTST    R  +++++
Sbjct:  573 GVTDVLFGDYGFTGKLPRTWFKNVEQLPMNIGDSHYDPLFPFDFGIRTKSAPGVVARSTSTGVIERPHMLSVV 645
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q6PQF3_SECCE (Cell wall beta-glucosidase OS=Secale cereale PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.083e-21
Identity = 46/50 (92.00%), Postives = 48/50 (96.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSES 150
            GVTD LFGDYGFSGKLARTWFKSVDQLPMNVGD HYDPLFPFGFGLT+E+
Sbjct:  573 GVTDALFGDYGFSGKLARTWFKSVDQLPMNVGDKHYDPLFPFGFGLTTEA 622
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q4F885_GOSHI (Endo-alpha-1,4-glucanase OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.639e-21
Identity = 45/52 (86.54%), Postives = 48/52 (92.31%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVR 156
            GV DVLFGDYGFSGKL RTWFK+VDQLPMNVGDPHYDPLFPFGFGLT+E  +
Sbjct:  574 GVADVLFGDYGFSGKLPRTWFKTVDQLPMNVGDPHYDPLFPFGFGLTTEPTK 625
[Close]
BLAST of EX570178 vs. TrEMBL
Match: B9SD66_RICCO (Hydrolase, hydrolyzing O-glycosyl compounds, putative OS=Ricinus communis GN=RCOM_1162380 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.639e-21
Identity = 44/53 (83.02%), Postives = 51/53 (96.23%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRD 159
            GV D+LFGDYGF+GKLARTWFK+VDQLPMNVGDPHYDPLFPFGFGLT++ V++
Sbjct:  580 GVADLLFGDYGFTGKLARTWFKTVDQLPMNVGDPHYDPLFPFGFGLTTKPVKN 632
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q7XAS3_GOSHI (Beta-D-glucosidase OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.132e-20
Identity = 44/49 (89.80%), Postives = 49/49 (100.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSE 147
            GV+DVLFGDYGF+GKLARTWFK+VDQLPMNVGDPHYDPLFPFGFGLT++
Sbjct:  575 GVSDVLFGDYGFTGKLARTWFKTVDQLPMNVGDPHYDPLFPFGFGLTTK 623
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q8RWR5_WHEAT (Beta-D-glucan exohydrolase OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.932e-20
Identity = 45/50 (90.00%), Postives = 47/50 (94.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSES 150
            GV D LFGDYGFSGKLARTWFKSVDQLPMNVGD HYDPLFPFGFGLT+E+
Sbjct:  573 GVADALFGDYGFSGKLARTWFKSVDQLPMNVGDKHYDPLFPFGFGLTTEA 622
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q42835_HORVU (Beta-D-glucan exohydrolase, isoenzyme ExoII OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.932e-20
Identity = 45/50 (90.00%), Postives = 47/50 (94.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSES 150
            GV DVLFGDYGFSGKLARTWFKS DQLPMNVGD HYDPLFPFGFGLT+E+
Sbjct:  573 GVADVLFGDYGFSGKLARTWFKSADQLPMNVGDKHYDPLFPFGFGLTTEA 622
[Close]
BLAST of EX570178 vs. TrEMBL
Match: A5BHD9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011685 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.932e-20
Identity = 44/51 (86.27%), Postives = 50/51 (98.04%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESV 153
            G+TDVLFGDYGFSGKL+RTWFK+VDQLPMNVGD HYDPLFPFGFGLT+++V
Sbjct:  127 GLTDVLFGDYGFSGKLSRTWFKNVDQLPMNVGDSHYDPLFPFGFGLTTKTV 177
[Close]
BLAST of EX570178 vs. TrEMBL
Match: Q2HVM0_MEDTR (Glycoside hydrolase, family 3, N-terminal; Glycoside hydrolase, family 3, C-terminal OS=Medicago truncatula GN=MtrDRAFT_AC148816g20v2 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.295e-20
Identity = 43/54 (79.63%), Postives = 49/54 (90.74%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRDL 162
            GV DVLFGDYGF+GKL RTWFKSVDQLPMNVGDPHYDP+FPFGFGLT++  + +
Sbjct:  576 GVADVLFGDYGFTGKLPRTWFKSVDQLPMNVGDPHYDPVFPFGFGLTTKPTKPI 629
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT5G20940.1 (| Symbols: | Glycosyl hydrolase family protein | chr5:7104076-7106653 REVERSE LENGTH=626)

HSP 1 Score: 98.9821 bits (245), Expect = 1.079e-21
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSE 147
            GV DVLFGDYGF+GKLARTWFK+VDQLPMNVGDPHYDPL+PFGFGL ++
Sbjct:  576 GVADVLFGDYGFTGKLARTWFKTVDQLPMNVGDPHYDPLYPFGFGLITK 624
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT5G04885.1 (| Symbols: | Glycosyl hydrolase family protein | chr5:1423369-1426628 FORWARD LENGTH=665)

HSP 1 Score: 95.5153 bits (236), Expect = 1.193e-20
Identity = 43/83 (51.81%), Postives = 60/83 (72.29%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRDLVTRSTS-TAEFVRACIITIMATLLTSLYL 246
            G+TD LFGD+GFSGKL  TWF++ +QLPM+ GD HYDPLF +G GL +ESV  +V RSTS +A   + C+ T++ +    L++
Sbjct:  576 GITDALFGDHGFSGKLPVTWFRNTEQLPMSYGDTHYDPLFAYGSGLETESVASIVARSTSASATNTKPCLYTVLVSATLCLFI 658
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT5G20950.2 (| Symbols: | Glycosyl hydrolase family protein | chr5:7107609-7110775 REVERSE LENGTH=624)

HSP 1 Score: 94.7449 bits (234), Expect = 2.036e-20
Identity = 41/49 (83.67%), Postives = 45/49 (91.84%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSE 147
            GV D LFGDYGF+GKLARTWFKSV QLPMNVGD HYDPL+PFGFGLT++
Sbjct:  572 GVADALFGDYGFTGKLARTWFKSVKQLPMNVGDRHYDPLYPFGFGLTTK 620
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT5G20950.1 (| Symbols: | Glycosyl hydrolase family protein | chr5:7107609-7110775 REVERSE LENGTH=624)

HSP 1 Score: 94.7449 bits (234), Expect = 2.036e-20
Identity = 41/49 (83.67%), Postives = 45/49 (91.84%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSE 147
            GV D LFGDYGF+GKLARTWFKSV QLPMNVGD HYDPL+PFGFGLT++
Sbjct:  572 GVADALFGDYGFTGKLARTWFKSVKQLPMNVGDRHYDPLYPFGFGLTTK 620
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G62710.1 (| Symbols: | Glycosyl hydrolase family protein | chr3:23197739-23200559 REVERSE LENGTH=650)

HSP 1 Score: 75.8702 bits (185), Expect = 9.786e-15
Identity = 32/46 (69.57%), Postives = 38/46 (82.61%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGL 138
            GV DVLFGD+ F+G L RTW K V QLPMNVGD +YDPL+PFG+G+
Sbjct:  603 GVADVLFGDHPFTGTLPRTWMKHVTQLPMNVGDKNYDPLYPFGYGI 648
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G47010.2 (| Symbols: | Glycosyl hydrolase family protein | chr3:17316758-17319518 REVERSE LENGTH=608)

HSP 1 Score: 73.1738 bits (178), Expect = 6.343e-14
Identity = 32/48 (66.67%), Postives = 37/48 (77.08%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTS 144
            G++DV+FGDY F GKL  +WFK VDQLP+N     YDPLFP GFGLTS
Sbjct:  558 GMSDVIFGDYDFKGKLPVSWFKRVDQLPLNAEANSYDPLFPLGFGLTS 605
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G47010.1 (| Symbols: | Glycosyl hydrolase family protein | chr3:17316758-17319518 REVERSE LENGTH=609)

HSP 1 Score: 73.1738 bits (178), Expect = 6.343e-14
Identity = 32/48 (66.67%), Postives = 37/48 (77.08%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTS 144
            G++DV+FGDY F GKL  +WFK VDQLP+N     YDPLFP GFGLTS
Sbjct:  559 GMSDVIFGDYDFKGKLPVSWFKRVDQLPLNAEANSYDPLFPLGFGLTS 606
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G47050.1 (| Symbols: | Glycosyl hydrolase family protein | chr3:17328092-17330857 REVERSE LENGTH=612)

HSP 1 Score: 72.0182 bits (175), Expect = 1.413e-13
Identity = 31/53 (58.49%), Postives = 38/53 (71.70%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSESVRD 159
            G+ DV+FGDY F GKL  TWFK VDQLP+++    Y PLFP GFGL  +SV +
Sbjct:  556 GIADVIFGDYDFRGKLPATWFKRVDQLPLDIESNGYLPLFPLGFGLNGDSVEN 608
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G47040.2 (| Symbols: | Glycosyl hydrolase family protein | chr3:17324231-17327105 REVERSE LENGTH=644)

HSP 1 Score: 70.0922 bits (170), Expect = 5.370e-13
Identity = 31/50 (62.00%), Postives = 35/50 (70.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSES 150
            G+TDV+FGDY F GKL  +WFK VDQLP+      YDPLFP GFGL   S
Sbjct:  591 GMTDVIFGDYDFEGKLPVSWFKRVDQLPLTADANSYDPLFPLGFGLNYNS 640
[Close]
BLAST of EX570178 vs. TAIR peptide
Match: AT3G47040.1 (| Symbols: | Glycosyl hydrolase family protein | chr3:17324231-17327105 REVERSE LENGTH=636)

HSP 1 Score: 70.0922 bits (170), Expect = 5.370e-13
Identity = 31/50 (62.00%), Postives = 35/50 (70.00%), Query Frame = 1 
Query:    1 GVTDVLFGDYGFSGKLARTWFKSVDQLPMNVGDPHYDPLFPFGFGLTSES 150
            G+TDV+FGDY F GKL  +WFK VDQLP+      YDPLFP GFGL   S
Sbjct:  583 GMTDVIFGDYDFEGKLPVSWFKRVDQLPLTADANSYDPLFPLGFGLNYNS 632
The following BLAST results are available for this feature:
BLAST of EX570178 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7T3B0_VITVI2.205e-2469.51Whole genome shotgun sequence of line PN40024, sca... [more]
B9RL34_RICCO2.980e-2163.01Periplasmic beta-glucosidase, putative OS=Ricinus ... [more]
Q6PQF3_SECCE5.083e-2192.00Cell wall beta-glucosidase OS=Secale cereale PE=2 ... [more]
Q4F885_GOSHI6.639e-2186.54Endo-alpha-1,4-glucanase OS=Gossypium hirsutum PE=... [more]
B9SD66_RICCO6.639e-2183.02Hydrolase, hydrolyzing O-glycosyl compounds, putat... [more]
Q7XAS3_GOSHI1.132e-2089.80Beta-D-glucosidase OS=Gossypium hirsutum PE=2 SV=1[more]
Q8RWR5_WHEAT1.932e-2090.00Beta-D-glucan exohydrolase OS=Triticum aestivum PE... [more]
Q42835_HORVU1.932e-2090.00Beta-D-glucan exohydrolase, isoenzyme ExoII OS=Hor... [more]
A5BHD9_VITVI1.932e-2086.27Putative uncharacterized protein OS=Vitis vinifera... [more]
Q2HVM0_MEDTR3.295e-2079.63Glycoside hydrolase, family 3, N-terminal; Glycosi... [more]
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BLAST of EX570178 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT5G20940.11.079e-2185.71| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT5G04885.11.193e-2051.81| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT5G20950.22.036e-2083.67| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT5G20950.12.036e-2083.67| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G62710.19.786e-1569.57| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G47010.26.343e-1466.67| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G47010.16.343e-1466.67| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G47050.11.413e-1358.49| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G47040.25.370e-1362.00| Symbols: | Glycosyl hydrolase family protein | ... [more]
AT3G47040.15.370e-1362.00| Symbols: | Glycosyl hydrolase family protein | ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002772Glycoside hydrolase, family 3, C-terminalPFAMPF01915Glyco_hydro_3_Ccoord: 2..47
score: 4.1
IPR002772Glycoside hydrolase, family 3, C-terminalSUPERFAMILY52279Beta-D-glucan exohydrolase, C-terminal domaincoord: 1..47
score: 3.1
NoneNo IPR availableGENE3D3.40.50.1700no descriptioncoord: 1..47
score: 9.1

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX570178 ID=EX570178; Name=EX570178; organism=Pisum sativum; type=EST; length=437bp
GGTGTGACAGATGTTCTATTTGGTGACTACGGTTTCAGTGGCAAACTTGC
AAGGACATGGTTCAAATCTGTAGATCAACTCCCAATGAATGTAGGAGATC
CTCACTATGATCCACTTTTTCCTTTTGGTTTCGGCCTAACATCCGAATCT
GTCAGAGACCTAGTAACAAGGTCAACTTCAACCGCTGAATTCGTAAGAGC
TTGTATAATTACCATTATGGCGACGCTACTGACGAGCTTATATTTAATTG
GTTGAGCAATGTATGTTTATGGGAACTTATTGGAGCTGGAGGCTAGAGAA
CTAACTTGGGATACTTCCTAGATTAGTCCTATTTTAGTCGAATTCATTGT
ATTATGACATTATTATTCATGAATTAGGACATTTTTTTCAGTACATATAC
ATTATTTATTAGACAAAACCTGATTCATGATCTTGAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR002772Glyco_hydro_3_C