EX570448

EST Overview
NameEX570448
Unique NameEX570448
TypeEST
OrganismPisum sativum (pea)
Sequence length707
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of EX570448 vs. TrEMBL
Match: D7MP40_ARALY (SEC14 cytosolic factor family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_494221 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.084e-16
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            K+QVLGP+Y   LLEAIDPSNLPTFLGGNC CSD+GGCL SD+GPW + +  + IQ
Sbjct:  224 KVQVLGPNYLGELLEAIDPSNLPTFLGGNCTCSDHGGCLFSDEGPWNDPDIKKKIQ 279
[Close]
BLAST of EX570448 vs. TrEMBL
Match: B9MUN4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_917530 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.084e-16
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQV 172
            KI VLG +Y S LLE ID SNLP+FLGGNC CSDYGGCL SD+GPW+N E +EM+QV
Sbjct:  242 KIHVLGCNYLSNLLEVIDQSNLPSFLGGNCTCSDYGGCLFSDKGPWQNPEVVEMLQV 298
[Close]
BLAST of EX570448 vs. TrEMBL
Match: B9GYA6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_852646 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.416e-16
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQV 172
            KI VLG +Y S LLE ID SNLP+FLGG+C CSDYGGCL SD+GPW+N E LEM+QV
Sbjct:  242 KIHVLGYNYLSNLLEVIDQSNLPSFLGGDCTCSDYGGCLFSDKGPWQNPEILEMLQV 298
[Close]
BLAST of EX570448 vs. TrEMBL
Match: B9SSF9_RICCO (Sec14 cytosolic factor, putative OS=Ricinus communis GN=RCOM_1061140 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.849e-16
Identity = 40/64 (62.50%), Postives = 48/64 (75.00%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQVCQYHEHI 193
            KIQVLG +Y S LLE ID SNLP+FLGG+C CSDYGGCL  D+GPW N E +EM+Q     E++
Sbjct:  242 KIQVLGSNYQSNLLEVIDASNLPSFLGGSCTCSDYGGCLFRDKGPWNNLEIVEMLQAVSATENV 305
[Close]
BLAST of EX570448 vs. TrEMBL
Match: Q9FGK6_ARATH (Similarity to phosphatidylinositol/phosphatidylcholine transfer protein OS=Arabidopsis thaliana GN=At5g47510 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 7.028e-16
Identity = 38/56 (67.86%), Postives = 45/56 (80.36%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            K+QVLGP+Y   LLEAI+PSNLPTFLGGNC CSD+GGCL SD+GPW +    E I+
Sbjct:  233 KVQVLGPNYLGELLEAIEPSNLPTFLGGNCTCSDHGGCLFSDEGPWNDPGIKEKIE 288
[Close]
BLAST of EX570448 vs. TrEMBL
Match: Q2A9T2_BRAOL (CRAL/TRIO domain containing protein OS=Brassica oleracea GN=25.t00016 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.199e-15
Identity = 39/56 (69.64%), Postives = 43/56 (76.79%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            K+QVLGP+Y   LLEAIDPSNLPTFLGGNC CSD GGCL SD+GPW +      IQ
Sbjct:  245 KVQVLGPNYLGELLEAIDPSNLPTFLGGNCTCSDRGGCLFSDEGPWNDPNIEAKIQ 300
[Close]
BLAST of EX570448 vs. TrEMBL
Match: D7TN50_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_154.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001318001 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.488e-15
Identity = 38/62 (61.29%), Postives = 47/62 (75.81%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQVCQYHE 187
            KI+VLG +Y S L+E IDPSNLP+FL GNC CS YGGCL SD+GPW + E +EM+Q    +E
Sbjct:  648 KIEVLGSNYQSNLVEFIDPSNLPSFLCGNCTCSGYGGCLFSDKGPWNDPEIIEMLQAAVENE 709
[Close]
BLAST of EX570448 vs. TrEMBL
Match: B9S6A7_RICCO (Sec14 cytosolic factor, putative OS=Ricinus communis GN=RCOM_0534520 PE=4 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 8.591e-14
Identity = 36/56 (64.29%), Postives = 42/56 (75.00%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            KI VLG  Y S LLE ID S LP FLGG C C+D GGC+ SD+GPWK+QE L+M+Q
Sbjct:  277 KIHVLGNKYQSKLLEIIDASELPEFLGGTCTCADQGGCMRSDKGPWKDQEILKMVQ 332
[Close]
BLAST of EX570448 vs. TrEMBL
Match: B9HRK3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_721963 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.500e-13
Identity = 35/55 (63.64%), Postives = 41/55 (74.55%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMI 166
            KI VLG  Y + LLE ID S LP FLGG C C+D GGCL+SD+GPWKN E L+M+
Sbjct:  292 KIHVLGNKYQTKLLEIIDTSELPEFLGGTCTCADQGGCLLSDKGPWKNPEILKMV 346
[Close]
BLAST of EX570448 vs. TrEMBL
Match: Q84YR9_ORYSJ (Putative Sec14 cytosolic factor (Phosphatidylinositol/phosphatidyl-choline transfer protein) OS=Oryza sativa subsp. japonica GN=OSJNBa0027N13.117 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.265e-13
Identity = 34/50 (68.00%), Postives = 40/50 (80.00%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQE 151
            KIQVLG +Y S +LEA+DPSNLP FLGG C CS  GGCL+ D+GPW +QE
Sbjct:  240 KIQVLGTNYLSTILEAVDPSNLPDFLGGTCTCSATGGCLLQDKGPWTDQE 289
[Close]
BLAST of EX570448 vs. SwissProt
Match: SEC14_SCHPO (Sec14 cytosolic factor OS=Schizosaccharomyces pombe GN=sec14 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.340e-8
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFL 157
            KI +LG +Y S LLE I   NLP  LGGNC C   GGC +SD GPW  ++++
Sbjct:  234 KIHILGSNYKSALLEQIPADNLPAKLGGNCQCP--GGCELSDAGPWHEEQWM 283
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT5G47510.1 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr5:19275048-19276999 FORWARD LENGTH=376)

HSP 1 Score: 88.1965 bits (217), Expect = 4.778e-18
Identity = 38/56 (67.86%), Postives = 45/56 (80.36%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            K+QVLGP+Y   LLEAI+PSNLPTFLGGNC CSD+GGCL SD+GPW +    E I+
Sbjct:  233 KVQVLGPNYLGELLEAIEPSNLPTFLGGNCTCSDHGGCLFSDEGPWNDPGIKEKIE 288
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT4G39170.1 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr4:18240887-18243621 FORWARD LENGTH=614)

HSP 1 Score: 77.7962 bits (190), Expect = 6.457e-15
Identity = 34/55 (61.82%), Postives = 41/55 (74.55%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMI 166
            KI VLG  Y S LLE ID S LP FLGG C C+D GGC++SD+GPWKN E ++M+
Sbjct:  291 KIHVLGCKYQSKLLEIIDSSELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMV 345
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT4G36490.1 (| Symbols: ATSFH12, SFH12 | SEC14-like 12 | chr4:17222099-17224808 FORWARD LENGTH=543)

HSP 1 Score: 75.8702 bits (185), Expect = 2.454e-14
Identity = 33/55 (60.00%), Postives = 40/55 (72.73%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMI 166
            KI VLG  Y S LLE ID S LP FLGG+C C+D GGC+ SD+GPWKN E ++ +
Sbjct:  261 KIHVLGNKYQSKLLEIIDESELPEFLGGSCTCADNGGCMRSDKGPWKNPEIMKRV 315
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT2G21520.2 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr2:9215956-9218953 FORWARD LENGTH=637)

HSP 1 Score: 75.8702 bits (185), Expect = 2.454e-14
Identity = 33/55 (60.00%), Postives = 41/55 (74.55%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMI 166
            KI VLG  Y S LLE ID + LP FLGG C C+D GGC++SD+GPWKN E ++M+
Sbjct:  297 KIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMV 351
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT2G21520.1 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr2:9215956-9218953 FORWARD LENGTH=633)

HSP 1 Score: 75.8702 bits (185), Expect = 2.454e-14
Identity = 33/55 (60.00%), Postives = 41/55 (74.55%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMI 166
            KI VLG  Y S LLE ID + LP FLGG C C+D GGC++SD+GPWKN E ++M+
Sbjct:  293 KIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMV 347
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT2G21540.3 (| Symbols: SFH3 | SEC14-like 3 | chr2:9220831-9223737 REVERSE LENGTH=542)

HSP 1 Score: 75.0998 bits (183), Expect = 4.185e-14
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            KI VLG  Y S LLE ID + LP FLGGNC C+D GGC+ SD+GPW + +  +M+Q
Sbjct:  278 KIHVLGNKYQSKLLEIIDSNELPEFLGGNCTCADKGGCMRSDKGPWNDPDIFKMVQ 333
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT2G21540.2 (| Symbols: ATSFH3, SFH3 | SEC14-like 3 | chr2:9220831-9223737 REVERSE LENGTH=548)

HSP 1 Score: 75.0998 bits (183), Expect = 4.185e-14
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            KI VLG  Y S LLE ID + LP FLGGNC C+D GGC+ SD+GPW + +  +M+Q
Sbjct:  278 KIHVLGNKYQSKLLEIIDSNELPEFLGGNCTCADKGGCMRSDKGPWNDPDIFKMVQ 333
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT2G21540.1 (| Symbols: ATSFH3, SFH3 | SEC14-like 3 | chr2:9220831-9223737 REVERSE LENGTH=548)

HSP 1 Score: 75.0998 bits (183), Expect = 4.185e-14
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            KI VLG  Y S LLE ID + LP FLGGNC C+D GGC+ SD+GPW + +  +M+Q
Sbjct:  278 KIHVLGNKYQSKLLEIIDSNELPEFLGGNCTCADKGGCMRSDKGPWNDPDIFKMVQ 333
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT4G39170.2 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr4:18240887-18243621 FORWARD LENGTH=612)

HSP 1 Score: 74.7146 bits (182), Expect = 5.466e-14
Identity = 33/52 (63.46%), Postives = 38/52 (73.08%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFL 157
            KI VLG  Y S LLE ID S LP FLGG C C+D GGC++SD+GPWKN E +
Sbjct:  291 KIHVLGCKYQSKLLEIIDSSELPEFLGGACTCADQGGCMLSDKGPWKNPEIM 342
[Close]
BLAST of EX570448 vs. TAIR peptide
Match: AT1G75370.2 (| Symbols: | Sec14p-like phosphatidylinositol transfer family protein | chr1:28276440-28279798 REVERSE LENGTH=668)

HSP 1 Score: 74.3294 bits (181), Expect = 7.139e-14
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:    2 KIQVLGPDYPSVLLEAIDPSNLPTFLGGNCICSDYGGCLMSDQGPWKNQEFLEMIQ 169
            KI V+G  Y + LLE ID S LP FLGG C C+D GGC+ SD+GPW + E L+M+Q
Sbjct:  296 KIHVIGNKYQNKLLEIIDASQLPDFLGGTCTCADRGGCMRSDKGPWNDPEILKMLQ 351
The following BLAST results are available for this feature:
BLAST of EX570448 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7MP40_ARALY1.084e-1669.64SEC14 cytosolic factor family protein OS=Arabidops... [more]
B9MUN4_POPTR1.084e-1671.93Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9GYA6_POPTR1.416e-1671.93Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SSF9_RICCO1.849e-1662.50Sec14 cytosolic factor, putative OS=Ricinus commun... [more]
Q9FGK6_ARATH7.028e-1667.86Similarity to phosphatidylinositol/phosphatidylcho... [more]
Q2A9T2_BRAOL1.199e-1569.64CRAL/TRIO domain containing protein OS=Brassica ol... [more]
D7TN50_VITVI3.488e-1561.29Whole genome shotgun sequence of line PN40024, sca... [more]
B9S6A7_RICCO8.591e-1464.29Sec14 cytosolic factor, putative OS=Ricinus commun... [more]
B9HRK3_POPTR2.500e-1363.64Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q84YR9_ORYSJ3.265e-1368.00Putative Sec14 cytosolic factor (Phosphatidylinosi... [more]
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BLAST of EX570448 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
SEC14_SCHPO2.340e-851.92Sec14 cytosolic factor OS=Schizosaccharomyces pomb... [more]
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BLAST of EX570448 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT5G47510.14.778e-1867.86| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
AT4G39170.16.457e-1561.82| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
AT4G36490.12.454e-1460.00| Symbols: ATSFH12, SFH12 | SEC14-like 12 | chr4:1... [more]
AT2G21520.22.454e-1460.00| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
AT2G21520.12.454e-1460.00| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
AT2G21540.34.185e-1457.14| Symbols: SFH3 | SEC14-like 3 | chr2:9220831-9223... [more]
AT2G21540.24.185e-1457.14| Symbols: ATSFH3, SFH3 | SEC14-like 3 | chr2:9220... [more]
AT2G21540.14.185e-1457.14| Symbols: ATSFH3, SFH3 | SEC14-like 3 | chr2:9220... [more]
AT4G39170.25.466e-1463.46| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
AT1G75370.27.139e-1457.14| Symbols: | Sec14p-like phosphatidylinositol tra... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251Cellular retinaldehyde-binding/triple function, C-terminalGENE3D3.40.525.10no descriptioncoord: 1..54
score: 7.1
IPR001251Cellular retinaldehyde-binding/triple function, C-terminalSUPERFAMILY52087CRAL/TRIO domaincoord: 1..61
score: 2.7
NoneNo IPR availablePANTHERPTHR23324SEC14 RELATED PROTEINcoord: 1..56
score: 2.9
NoneNo IPR availablePANTHERPTHR23324:SF5SEC14 CYTOSOLIC FACTOR-RELATEDcoord: 1..56
score: 2.9

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX570448 ID=EX570448; Name=EX570448; organism=Pisum sativum; type=EST; length=707bp
GAAAATTCAAGTTTTGGGTCCTGATTATCCTAGTGTGCTGCTAGAGGCTA
TTGATCCAAGTAACTTACCAACTTTTTTGGGTGGTAATTGCATATGTTCT
GATTACGGAGGTTGCTTGATGAGTGATCAAGGTCCATGGAAAAATCAAGA
GTTCTTAGAAATGATTCAGGTTTGTCAATATCATGAACACATTATTTGCT
GGTAGAAATAACTCACAAGGTCGCATAATTATTCGCGCAGACAGTTTCTT
TAACAGAAGAAACCGACGGTGCGTGCGAAAACAACAATGTGGTTTCAGAA
GGATTCTTGATGCACAAAAAGGTTGACATGAAAAACAAAGACGATGTTAG
AACAGATCTACCATGTAGGTTAGCATTACAAAAAATCGATTGCCTTGAAG
ATAGTCTGGGAGACATCAAGAATAAAATCAAGACGCTGGAAGATGCACTT
CAAGAAACTAAGATGGCATTGAAAGAAATTGGACAACATATAGAACAACA
GAAAATGTGAATTTCTCAAGTGTAAACAAAATTGCAATGAGACAGCAACT
TATATTCCTATGATCGGTTATGATCAATGAAAGTTAAAAGAAGACTGTTA
ATATTATATCATAGTGGTTAATGGTTGTGTAGCAACCTAAAATAAAATTT
GGTAAGTGTCTCTGACTAGGTGGTGGAAATAGTTTAAACCTATAAGGTTG
ATGATCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR001251CRAL-TRIO_dom