EX570699

EST Overview


Name	EX570699
Unique Name	EX570699
Type	EST
Organism	Pisum sativum (pea)
Sequence length	613

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX570699 vs. TrEMBL
Match: A8VYM3_FRAAN (Chloroplast fructose-1,6-bisphosphatase OS=Fragaria ananassa PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.078e-67
Identity = 90/95 (94.74%), Postives = 94/95 (98.95%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            +KIP RGQIYSVNDARYFDWPEGLRKYIDTVRQGKG+YPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSF+VEQAGG+GSDGK
Sbjct:  234 LKIPPRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGRYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFLVEQAGGKGSDGK 328

HSP 2 Score: 88.9669 bits (219), Expect = 1.078e-67
Identity = 41/45 (91.11%), Postives = 45/45 (100.00%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K+RILS+QP+KLHQRLPLFLGSLEDM+ELESYGDVQQKVNPGYEV
Sbjct:  328 KNRILSIQPIKLHQRLPLFLGSLEDMDELESYGDVQQKVNPGYEV 372

[Close]

BLAST of EX570699 vs. TrEMBL
Match: B9IJ66_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_577299 PE=3 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 1.079e-67
Identity = 89/96 (92.71%), Postives = 94/96 (97.92%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKA 290
            IKIP RGQIYSVNDARYFDWPEGLR+YIDTVRQGKG+YPKKYSARYICSLVAD HRTLLYGGVAMNPR+HLRLVYEANPLSF+VEQAGGRGSDGK+
Sbjct:  219 IKIPPRGQIYSVNDARYFDWPEGLRQYIDTVRQGKGRYPKKYSARYICSLVADFHRTLLYGGVAMNPRNHLRLVYEANPLSFLVEQAGGRGSDGKS 314

HSP 2 Score: 91.6633 bits (226), Expect = 1.079e-67
Identity = 44/45 (97.78%), Postives = 45/45 (100.00%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            KSRILS+QPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV
Sbjct:  313 KSRILSMQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 357

[Close]

BLAST of EX570699 vs. TrEMBL
Match: B9T3E7_RICCO (Fructose-1,6-bisphosphatase, putative OS=Ricinus communis GN=RCOM_0449370 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 9.826e-66
Identity = 88/95 (92.63%), Postives = 92/95 (96.84%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            IKIP RGQIYSVNDARYFDWPEGLR+YIDTVRQGKG+YPKKYSARYICSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK
Sbjct:  265 IKIPSRGQIYSVNDARYFDWPEGLRQYIDTVRQGKGRYPKKYSARYICSLVADFHRTLMYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGK 359

HSP 2 Score: 85.5001 bits (210), Expect = 9.826e-66
Identity = 41/45 (91.11%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K RILS+QPVKLHQRLPLFLGS ED+EELESYGDVQQKVNPGYEV
Sbjct:  359 KHRILSMQPVKLHQRLPLFLGSPEDIEELESYGDVQQKVNPGYEV 403

[Close]

BLAST of EX570699 vs. TrEMBL
Match: Q655Y9_ORYSJ (Os06g0664200 protein OS=Oryza sativa subsp. japonica GN=P0473H04.3-1 PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.831e-65
Identity = 85/96 (88.54%), Postives = 93/96 (96.88%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKA 290
            I+IP RGQIYSVNDARYFDWPEGLRKYIDT+RQGKG++PKKYSARY+CSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK+
Sbjct:  263 IQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVCSLVADFHRTLIYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGKS 358

HSP 2 Score: 86.2705 bits (212), Expect = 4.831e-65
Identity = 41/45 (91.11%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            KSRILS+QPVKLHQRLPLFLG +EDM ELESYGDVQQKVNPGYEV
Sbjct:  357 KSRILSIQPVKLHQRLPLFLGGMEDMLELESYGDVQQKVNPGYEV 401

[Close]

BLAST of EX570699 vs. TrEMBL
Match: B9FQD6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_22273 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.836e-65
Identity = 85/96 (88.54%), Postives = 93/96 (96.88%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKA 290
            I+IP RGQIYSVNDARYFDWPEGLRKYIDT+RQGKG++PKKYSARY+CSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK+
Sbjct:  221 IQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVCSLVADFHRTLIYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGKS 316

HSP 2 Score: 86.2705 bits (212), Expect = 4.836e-65
Identity = 41/45 (91.11%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            KSRILS+QPVKLHQRLPLFLG +EDM ELESYGDVQQKVNPGYEV
Sbjct:  315 KSRILSIQPVKLHQRLPLFLGGMEDMLELESYGDVQQKVNPGYEV 359

[Close]

BLAST of EX570699 vs. TrEMBL
Match: B8B117_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_24019 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.838e-65
Identity = 85/96 (88.54%), Postives = 93/96 (96.88%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKA 290
            I+IP RGQIYSVNDARYFDWPEGLRKYIDT+RQGKG++PKKYSARY+CSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK+
Sbjct:  205 IQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVCSLVADFHRTLIYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGKS 300

HSP 2 Score: 86.2705 bits (212), Expect = 4.838e-65
Identity = 41/45 (91.11%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            KSRILS+QPVKLHQRLPLFLG +EDM ELESYGDVQQKVNPGYEV
Sbjct:  299 KSRILSIQPVKLHQRLPLFLGGMEDMLELESYGDVQQKVNPGYEV 343

[Close]

BLAST of EX570699 vs. TrEMBL
Match: D7U658_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_114.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012513001 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 1.072e-64
Identity = 88/95 (92.63%), Postives = 92/95 (96.84%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            IKIP RGQIYSVNDARYFDWP+GLR+YIDTVRQGKGKYPKKYSARYICSLVAD HRTL+YGGVAMNPR HLRLVYEANPLSF+VEQAGGRGSDGK
Sbjct:  224 IKIPPRGQIYSVNDARYFDWPQGLRQYIDTVRQGKGKYPKKYSARYICSLVADFHRTLMYGGVAMNPRSHLRLVYEANPLSFLVEQAGGRGSDGK 318

HSP 2 Score: 83.1889 bits (204), Expect = 1.072e-64
Identity = 40/45 (88.89%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K RILS+QPVKLHQRLPLFLGS ED+EELESYG+VQQKVNPGYEV
Sbjct:  318 KIRILSIQPVKLHQRLPLFLGSPEDIEELESYGNVQQKVNPGYEV 362

[Close]

BLAST of EX570699 vs. TrEMBL
Match: B6SRX9_MAIZE (Fructose-1,6-bisphosphatase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.376e-64
Identity = 85/95 (89.47%), Postives = 92/95 (96.84%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            I+IP RGQIYSVNDARYFDWPEGLRKYIDT+RQGKG++PKKYSARY+CSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK
Sbjct:  260 IQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVCSLVADFHRTLIYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGK 354

HSP 2 Score: 84.3445 bits (207), Expect = 2.376e-64
Identity = 39/45 (86.67%), Postives = 44/45 (97.78%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K+RIL++QPVKLHQRLPLFLGS++DM ELESYGDVQQKVNPGYEV
Sbjct:  354 KNRILTIQPVKLHQRLPLFLGSMDDMLELESYGDVQQKVNPGYEV 398

[Close]

BLAST of EX570699 vs. TrEMBL
Match: C5Z7C5_SORBI (Putative uncharacterized protein Sb10g026540 OS=Sorghum bicolor GN=Sb10g026540 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.376e-64
Identity = 85/95 (89.47%), Postives = 92/95 (96.84%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            I+IP RGQIYSVNDARYFDWPEGLRKYIDT+RQGKG++PKKYSARY+CSLVAD HRTL+YGGVAMNPRDHLRLVYEANPLSF+ EQAGGRGSDGK
Sbjct:  258 IQIPPRGQIYSVNDARYFDWPEGLRKYIDTIRQGKGQHPKKYSARYVCSLVADFHRTLIYGGVAMNPRDHLRLVYEANPLSFLAEQAGGRGSDGK 352

HSP 2 Score: 84.3445 bits (207), Expect = 2.376e-64
Identity = 39/45 (86.67%), Postives = 44/45 (97.78%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K+RIL++QPVKLHQRLPLFLGS++DM ELESYGDVQQKVNPGYEV
Sbjct:  352 KNRILNIQPVKLHQRLPLFLGSIDDMLELESYGDVQQKVNPGYEV 396

[Close]

BLAST of EX570699 vs. TrEMBL
Match: Q9FMF1_ARATH (AT5g64380/MSJ1_22 OS=Arabidopsis thaliana GN=At5g64380 PE=2 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 1.168e-63
Identity = 88/97 (90.72%), Postives = 92/97 (94.85%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKAG 293
            IKIP RGQIYSVNDARYFDWPEGLRKYIDTVRQGKG+ PKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYE NPL+F+VEQAGG+ SDGK G
Sbjct:  266 IKIPTRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGQNPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEGNPLAFLVEQAGGKSSDGKRG 362

HSP 2 Score: 80.8777 bits (198), Expect = 1.168e-63
Identity = 39/45 (86.67%), Postives = 41/45 (91.11%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K  ILS+QPVKLHQRLPLFLGSLED+ ELESYGDVQQ VNPGYEV
Sbjct:  360 KRGILSIQPVKLHQRLPLFLGSLEDVAELESYGDVQQTVNPGYEV 404

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_ARATH (Fructose-1,6-bisphosphatase, chloroplastic OS=Arabidopsis thaliana GN=FBP PE=1 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 5.732e-30
Identity = 57/100 (57.00%), Postives = 68/100 (68.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            I+IP  G+IYS N+  Y  W + L+KYID ++   G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  285 IEIPKAGRIYSFNEGNYQMWDDKLKKYIDDLKD-PGPTGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 383

HSP 2 Score: 45.8246 bits (107), Expect = 5.732e-30
Identity = 17/31 (54.84%), Postives = 27/31 (87.10%), Query Frame = 2

Query:  287 SRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            SR+L +QP ++HQR+PL++GS E++E+LE Y
Sbjct:  385 SRVLDIQPTEIHQRVPLYIGSTEEVEKLEKY 415

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_BRANA (Fructose-1,6-bisphosphatase, chloroplastic OS=Brassica napus GN=FBP PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.733e-30
Identity = 58/100 (58.00%), Postives = 68/100 (68.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            I+IP  G+IYS N+  Y  W E L+KYID ++   G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  279 IEIPKAGKIYSFNEGNYQMWDENLKKYIDDLKD-PGPSGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 377

HSP 2 Score: 43.8986 bits (102), Expect = 5.733e-30
Identity = 16/30 (53.33%), Postives = 26/30 (86.67%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            R+L +QP ++HQR+PL++GS E++E+LE Y
Sbjct:  380 RVLDIQPTEIHQRVPLYIGSKEEVEKLEKY 409

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_SPIOL (Fructose-1,6-bisphosphatase, chloroplastic OS=Spinacia oleracea PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 9.717e-30
Identity = 55/100 (55.00%), Postives = 70/100 (70.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            I+IP  G+IYS N+  Y  W + L+KY+D +++  G+  K YS+RYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  283 IQIPKAGKIYSFNEGNYKMWDDKLKKYMDDLKE-PGESQKPYSSRYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 381

HSP 2 Score: 45.4394 bits (106), Expect = 9.717e-30
Identity = 17/30 (56.67%), Postives = 27/30 (90.00%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            RIL +QP ++HQR+PL++GS+E++E+LE Y
Sbjct:  384 RILDIQPTEIHQRVPLYIGSVEEVEKLEKY 413

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_PEA (Fructose-1,6-bisphosphatase, chloroplastic OS=Pisum sativum GN=FBP PE=1 SV=2)

HSP 1 Score: 107.842 bits (268), Expect = 9.720e-30
Identity = 57/100 (57.00%), Postives = 69/100 (69.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            ++IP  G+IYS N+  Y  W E L+KYID +++  G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  275 LQIPKSGEIYSFNEGNYKLWDENLKKYIDDLKE-PGPSGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDKKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 373

HSP 2 Score: 43.1282 bits (100), Expect = 9.720e-30
Identity = 15/30 (50.00%), Postives = 26/30 (86.67%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            R+L +QP ++HQR+PL++GS E++E++E Y
Sbjct:  376 RVLDIQPTEIHQRVPLYIGSTEEVEKVEKY 405

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_ORYSJ (Fructose-1,6-bisphosphatase, chloroplastic OS=Oryza sativa subsp. japonica GN=Os03g0267300 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.122e-27
Identity = 53/100 (53.00%), Postives = 69/100 (69.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDH------LRLVYEANPLSFIVEQAGGRGSDG 284
            ++IP  G+IY+ N+  Y  W + L+ Y+D++++  G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  274 VQIPKAGKIYAFNEGNYALWDDKLKSYMDSLKE-PGPSGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDQKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 372

HSP 2 Score: 40.817 bits (94), Expect = 1.122e-27
Identity = 14/30 (46.67%), Postives = 26/30 (86.67%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            RIL + P ++HQR+PL++GS+E++E++E +
Sbjct:  375 RILDIMPTEIHQRVPLYIGSVEEVEKVEKF 404

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16PA_CHLT3 (Fructose-1,6-bisphosphatase class 1 OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) GN=fbp PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 4.204e-27
Identity = 53/101 (52.48%), Postives = 70/101 (69.31%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGV------AMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            IK P RG+IYS+N+  Y  + +G++KYI  ++Q      + YSARYI S VAD HR LLYGG+      A +P+  LRL+YEANPL+FI EQAGGR S+G+
Sbjct:  197 IKTPKRGKIYSMNEGYYRYFDDGIKKYIKYLQQEDKASKRPYSARYIGSCVADFHRNLLYGGIFIYPRTAKSPKGKLRLMYEANPLAFICEQAGGRASNGR 297

HSP 2 Score: 39.2762 bits (90), Expect = 4.204e-27
Identity = 16/32 (50.00%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            + RIL ++P +LHQR PLF+GS ED++  E +
Sbjct:  297 RERILDIKPTELHQRTPLFIGSEEDVKIAEEF 328

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_WHEAT (Fructose-1,6-bisphosphatase, chloroplastic OS=Triticum aestivum GN=FBP PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.203e-26
Identity = 52/100 (52.00%), Postives = 68/100 (68.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDH------LRLVYEANPLSFIVEQAGGRGSDG 284
            ++IP  G+IYS N+  Y  W + L+KY+D++++  G   K YSARYI SLV D HRT+LYGG+   P D       LRL+YE  P+SFI EQAGG+GSDG
Sbjct:  275 VQIPKSGKIYSFNEGNYALWDDKLKKYMDSLKE-PGTSGKPYSARYIGSLVGDFHRTMLYGGIYGYPSDQKSKNGKLRLLYECAPMSFIAEQAGGKGSDG 373

HSP 2 Score: 38.891 bits (89), Expect = 1.203e-26
Identity = 13/30 (43.33%), Postives = 25/30 (83.33%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            R+L + P  +HQR+PL++GS+E++E++E +
Sbjct:  376 RVLDIMPTAVHQRVPLYVGSVEEVEKVEKF 405

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16P1_SOYBN (Fructose-1,6-bisphosphatase, chloroplastic OS=Glycine max GN=FBP PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 3.702e-25
Identity = 54/100 (54.00%), Postives = 69/100 (69.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            ++IP  G+IY+ N+  Y  W E L+KYID ++   G+  K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P++FIVEQAGG+G+DG
Sbjct:  273 LQIPRAGKIYAFNEGNYQLWDEKLKKYIDDLKD-PGQSGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDKKSKNGKLRLLYECAPINFIVEQAGGKGTDG 371

HSP 2 Score: 32.3426 bits (72), Expect = 3.702e-25
Identity = 13/30 (43.33%), Postives = 24/30 (80.00%), Query Frame = 2

Query:  290 RILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            ++L LQ  ++HQR+PL++G  E++E++E Y
Sbjct:  373 QVLRLQGTEIHQRVPLYIG--EEVEKVEKY 400

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16PA_PHOPR (Fructose-1,6-bisphosphatase class 1 OS=Photobacterium profundum GN=fbp PE=3 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 4.834e-25
Identity = 48/101 (47.52%), Postives = 68/101 (67.33%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGV------AMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            ++IP  GQIYS+N+  Y  +P+G++KYI   ++      + Y++RYI SLV+D HR LL GG+      AM P   LRL+YE NP++F++EQAGG  SDGK
Sbjct:  199 MQIPEDGQIYSINEGNYIRFPQGVKKYIKFCQEDVPADNRPYTSRYIGSLVSDFHRNLLKGGIYMYPSTAMYPNGKLRLLYECNPMAFLMEQAGGVASDGK 299

HSP 2 Score: 38.5058 bits (88), Expect = 4.834e-25
Identity = 15/32 (46.88%), Postives = 25/32 (78.12%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            K+RIL + P +LHQR+P F+GS   ++++ES+
Sbjct:  299 KNRILDITPTELHQRVPFFVGSTNMVKQVESF 330

[Close]

BLAST of EX570699 vs. SwissProt
Match: F16PA_SERP5 (Fructose-1,6-bisphosphatase class 1 OS=Serratia proteamaculans (strain 568) GN=fbp PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 6.292e-25
Identity = 45/101 (44.55%), Postives = 66/101 (65.35%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGV------AMNPRDHLRLVYEANPLSFIVEQAGGRGSDGK 287
            ++ P  G +YS+N+  Y  +P G++KYI   ++      + Y++RYI SLVAD HR LL GG+      A +P+  LRL+YE NP++F+ EQAGG+ SDGK
Sbjct:  196 VRFPATGNMYSINEGNYIKFPRGVKKYIKYCQEQDEATQRPYTSRYIGSLVADFHRNLLKGGIYIYPSTASHPQGKLRLLYECNPMAFLAEQAGGKASDGK 296

HSP 2 Score: 38.1206 bits (87), Expect = 6.292e-25
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            K+RIL + PVKLHQR P F+G+   +E+ E +
Sbjct:  296 KNRILDITPVKLHQRAPFFVGTKSMVEDAERF 327

[Close]

BLAST of EX570699 vs. TAIR peptide
Match: AT5G64380.1 (| Symbols: | Inositol monophosphatase family protein | chr5:25741342-25743024 FORWARD LENGTH=404)

HSP 1 Score: 187.578 bits (475), Expect = 8.573e-66
Identity = 88/97 (90.72%), Postives = 92/97 (94.85%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEANPLSFIVEQAGGRGSDGKAG 293
            IKIP RGQIYSVNDARYFDWPEGLRKYIDTVRQGKG+ PKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYE NPL+F+VEQAGG+ SDGK G
Sbjct:  266 IKIPTRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGQNPKKYSARYICSLVADLHRTLLYGGVAMNPRDHLRLVYEGNPLAFLVEQAGGKSSDGKRG 362

HSP 2 Score: 80.8777 bits (198), Expect = 8.573e-66
Identity = 39/45 (86.67%), Postives = 41/45 (91.11%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVNPGYEV 418
            K  ILS+QPVKLHQRLPLFLGSLED+ ELESYGDVQQ VNPGYEV
Sbjct:  360 KRGILSIQPVKLHQRLPLFLGSLEDVAELESYGDVQQTVNPGYEV 404

[Close]

BLAST of EX570699 vs. TAIR peptide
Match: AT3G54050.2 (| Symbols: HCEF1 | high cyclic electron flow 1 | chr3:20016951-20018527 FORWARD LENGTH=417)

HSP 1 Score: 105.916 bits (263), Expect = 6.031e-31
Identity = 57/100 (57.00%), Postives = 68/100 (68.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            I+IP  G+IYS N+  Y  W + L+KYID ++   G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  285 IEIPKAGRIYSFNEGNYQMWDDKLKKYIDDLKD-PGPTGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 383

HSP 2 Score: 45.8246 bits (107), Expect = 6.031e-31
Identity = 17/31 (54.84%), Postives = 27/31 (87.10%), Query Frame = 2

Query:  287 SRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            SR+L +QP ++HQR+PL++GS E++E+LE Y
Sbjct:  385 SRVLDIQPTEIHQRVPLYIGSTEEVEKLEKY 415

[Close]

BLAST of EX570699 vs. TAIR peptide
Match: AT3G54050.1 (| Symbols: HCEF1 | high cyclic electron flow 1 | chr3:20016951-20018527 FORWARD LENGTH=417)

HSP 1 Score: 105.916 bits (263), Expect = 6.031e-31
Identity = 57/100 (57.00%), Postives = 68/100 (68.00%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDG 284
            I+IP  G+IYS N+  Y  W + L+KYID ++   G   K YSARYI SLV D HRTLLYGG+   PRD       LRL+YE  P+SFIVEQAGG+GSDG
Sbjct:  285 IEIPKAGRIYSFNEGNYQMWDDKLKKYIDDLKD-PGPTGKPYSARYIGSLVGDFHRTLLYGGIYGYPRDAKSKNGKLRLLYECAPMSFIVEQAGGKGSDG 383

HSP 2 Score: 45.8246 bits (107), Expect = 6.031e-31
Identity = 17/31 (54.84%), Postives = 27/31 (87.10%), Query Frame = 2

Query:  287 SRILSLQPVKLHQRLPLFLGSLEDMEELESY 379
            SR+L +QP ++HQR+PL++GS E++E+LE Y
Sbjct:  385 SRVLDIQPTEIHQRVPLYIGSTEEVEKLEKY 415

[Close]

BLAST of EX570699 vs. TAIR peptide
Match: AT1G43670.1 (| Symbols: | Inositol monophosphatase family protein | chr1:16468184-16470347 FORWARD LENGTH=341)

HSP 1 Score: 87.8113 bits (216), Expect = 2.640e-24
Identity = 47/101 (46.53%), Postives = 64/101 (63.37%), Query Frame = 3

Query:    3 IKIPLRGQIYSVNDARYFDWPEGLRKYIDTVRQGKGKYPKKYSARYICSLVADLHRTLLYGGVAMNPRD------HLRLVYEANPLSFIVEQAGGRGSDGK 287
            IKIP +G IYSVN+    +W     KY++  +  K   P K S RY+ S+VAD+HRTLLYGG+ + P D       LR++YE  P+SF++EQAGG+   GK
Sbjct:  203 IKIPNKGNIYSVNEGNAQNWDGPTTKYVEKCKFPKDGSPAK-SLRYVGSMVADVHRTLLYGGIFLYPADKKSPNGKLRVLYEVFPMSFLMEQAGGQAFTGK 302

HSP 2 Score: 41.5874 bits (96), Expect = 2.640e-24
Identity = 18/40 (45.00%), Postives = 28/40 (70.00%), Query Frame = 2

Query:  284 KSRILSLQPVKLHQRLPLFLGSLEDMEELESYGDVQQKVN 403
            K R L L P K+H+R P+FLGS +D+EE+++    ++K N
Sbjct:  302 KKRALDLVPEKIHERSPIFLGSYDDVEEIKALYAEEEKKN 341

The following BLAST results are available for this feature:

BLAST of EX570699 vs. TrEMBL
BLAST of EX570699 vs. SwissProt
BLAST of EX570699 vs. TAIR peptide

BLAST of EX570699 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A8VYM3_FRAAN	1.078e-67	94.74	Chloroplast fructose-1,6-bisphosphatase OS=Fragari...	[more]
B9IJ66_POPTR	1.079e-67	92.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T3E7_RICCO	9.826e-66	92.63	Fructose-1,6-bisphosphatase, putative OS=Ricinus c...	[more]
Q655Y9_ORYSJ	4.831e-65	88.54	Os06g0664200 protein OS=Oryza sativa subsp. japoni...	[more]
B9FQD6_ORYSJ	4.836e-65	88.54	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8B117_ORYSI	4.838e-65	88.54	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7U658_VITVI	1.072e-64	92.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
B6SRX9_MAIZE	2.376e-64	89.47	Fructose-1,6-bisphosphatase OS=Zea mays PE=2 SV=1	[more]
C5Z7C5_SORBI	2.376e-64	89.47	Putative uncharacterized protein Sb10g026540 OS=So...	[more]
Q9FMF1_ARATH	1.168e-63	90.72	AT5g64380/MSJ1_22 OS=Arabidopsis thaliana GN=At5g6...	[more]

BLAST of EX570699 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
F16P1_ARATH	5.732e-30	57.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Arab...	[more]
F16P1_BRANA	5.733e-30	58.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Bras...	[more]
F16P1_SPIOL	9.717e-30	55.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Spin...	[more]
F16P1_PEA	9.720e-30	57.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Pisu...	[more]
F16P1_ORYSJ	1.122e-27	53.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Oryz...	[more]
F16PA_CHLT3	4.204e-27	52.48	Fructose-1,6-bisphosphatase class 1 OS=Chloroherpe...	[more]
F16P1_WHEAT	1.203e-26	52.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Trit...	[more]
F16P1_SOYBN	3.702e-25	54.00	Fructose-1,6-bisphosphatase, chloroplastic OS=Glyc...	[more]
F16PA_PHOPR	4.834e-25	47.52	Fructose-1,6-bisphosphatase class 1 OS=Photobacter...	[more]
F16PA_SERP5	6.292e-25	44.55	Fructose-1,6-bisphosphatase class 1 OS=Serratia pr...	[more]

BLAST of EX570699 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G64380.1	8.573e-66	90.72	\| Symbols: \| Inositol monophosphatase family prot...	[more]
AT3G54050.2	6.031e-31	57.00	\| Symbols: HCEF1 \| high cyclic electron flow 1 \| c...	[more]
AT3G54050.1	6.031e-31	57.00	\| Symbols: HCEF1 \| high cyclic electron flow 1 \| c...	[more]
AT1G43670.1	2.640e-24	46.53	\| Symbols: \| Inositol monophosphatase family prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000146	Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase	PANTHER	PTHR11556	FRUCTOSE-1,6-BISPHOSPHATASE-RELATED	coord: 1..95 score: 5.5
IPR000146	Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase	PFAM	PF00316	FBPase	coord: 1..96 score: 2.7
None	No IPR available	GENE3D	3.40.190.80	no description	coord: 1..96 score: 6.5
None	No IPR available	PANTHER	PTHR11556:SF1	FRUCTOSE-1,6-BISPHOSPHATASE	coord: 1..95 score: 5.5
None	No IPR available	SUPERFAMILY	56655	Carbohydrate phosphatase	coord: 1..113 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570699 ID=EX570699; Name=EX570699; organism=Pisum sativum; type=EST; length=613bp
ACATTAAAATTCCTCTCCGCGGGCAAATATATTCGGTGAATGATGCGAGA
TATTTTGATTGGCCTGAAGGTTTAAGGAAATATATAGATACTGTGAGACA
AGGAAAAGGTAAATATCCTAAGAAGTACTCAGCACGGTATATATGTTCTC
TGGTGGCGGATCTCCACCGAACTTTGTTGTATGGAGGTGTGGCTATGAAT
CCGAGGGACCATCTTCGTCTTGTATATGAAGCAAACCCTCTTAGTTTCAT
TGTGGAGCAAGCTGGTGGAAGAGGGTCTGATGGAAAAGCAGGATTCTTTC
ACTTCAACCGGTTAAACTTCACCAAAGACTTCCTCTCTTTTTAGGGAGTT
TGGAAGACATGGAAGAGTTGGAAAGTTATGGAGATGTACAACAAAAAGTA
AATCCTGGTTATGAGGTTTGAGGCTAGAATATTGAAATTTTAAATATTCA
TCCTTCATGTTTCCTAACTAATAACAAATCTGTGTATTGTTAGAATGAGA
CCTTGATTTGTATGGTATATAAGTTACAAGAGTTATGAATTTACATATTT
ACATATATGCTTATAACATGAGAATAATAAGAAAATGACATGGCAAGACA
AACTTTTGTATTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0042578	phosphoric ester hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000146	FBPase_class-1

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